← return to the list of all guides

Contents:

PCR primers for off-targets of AAAGGACATGGTTATGTTTC TGG

In the table below, Illumina Nextera Handle sequences have been added and highlighted in bold. Primers for the on-target have been added for convenience. The table below is sorted by the CFD off-target score. Sites with very low CFD scores < 0.02 are unlikely to be cleaved, see our study Haeussler et al. 2016, Figure 2.

In the protocol by Matthew Canver, Harvard, two PCRs are run: one PCR to amplify the potential off-target, then a second PCR to extend the handles with Illumina barcodes. Please click here to download the protocol. Alternatively, you can have a look at Fu et al, 2014.

If a primer was not found, the reason is usually that the region around the off-target is too repetitive. To avoid unspecific primers, all repeats are masked for the primer design (not for off-target search). If you think that we should change the parameters here or should use different primer3 settings, please let us know.

Maximum amplicon length:     Primer Tm:

NamePrimer Sequence Tm CFD Score
ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52313855_F Primer3: not found at this Tm N.d. 1.00
ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52313855_R Primer3: not found at this Tm N.d. 1.00
mm3_intergenic_Gm3307|Otud7a_chr7_63614814_F TCGTCGGCAGCGTCACTCAAAGTTGGCCCCTAATGA 59.6 0.68
mm3_intergenic_Gm3307|Otud7a_chr7_63614814_R GTCTCGTGGGCTCGGTGTTGTAAGCTTCTTGGCTCTG 58.8 0.68
mm4_intergenic_Cd274|Pdcd1lg2_chr19_29400397_F TCGTCGGCAGCGTCTGCAGACCCTTGTAAGTGGA 58.5 0.65
mm4_intergenic_Cd274|Pdcd1lg2_chr19_29400397_R GTCTCGTGGGCTCGGGAAAGCTTTGGATCAGGCCAC 59.7 0.65
mm4_intergenic_Tmc1|Zfand5_chr19_21074030_F TCGTCGGCAGCGTCTCAGAGAAATGGAGGAGAAACC 57.4 0.59
mm4_intergenic_Tmc1|Zfand5_chr19_21074030_R GTCTCGTGGGCTCGGTCCCAGAAAACAAATTCTTCCCA 58.4 0.59
mm4_intron_Dnah8_chr17_30793736_F TCGTCGGCAGCGTCTGTGAGCCATTAGAGATGTGAA 57.1 0.53
mm4_intron_Dnah8_chr17_30793736_R GTCTCGTGGGCTCGGTCCAACACGTCTGACAACTGT 59.7 0.53
mm4_intergenic_Gm26235|Gm25392_chr18_18578527_F TCGTCGGCAGCGTCAGCATTAAATGTGGCTTCATGCA 59.8 0.46
mm4_intergenic_Gm26235|Gm25392_chr18_18578527_R GTCTCGTGGGCTCGGAGGGAAGAATTAAGAGGTAAGCAA 57.6 0.46
mm4_intergenic_Gm13191|Frmd4a_chr2_3970891_F Primer3: not found at this Tm N.d. 0.45
mm4_intergenic_Gm13191|Frmd4a_chr2_3970891_R Primer3: not found at this Tm N.d. 0.45
mm4_intergenic_1700003H04Rik|Gm23673_chr3_124614934_F Primer3: not found at this Tm N.d. 0.43
mm4_intergenic_1700003H04Rik|Gm23673_chr3_124614934_R Primer3: not found at this Tm N.d. 0.43
mm4_intergenic_Gm24601|Gm26501_chr6_95000924_F Primer3: not found at this Tm N.d. 0.40
mm4_intergenic_Gm24601|Gm26501_chr6_95000924_R Primer3: not found at this Tm N.d. 0.40
mm4_intergenic_Ifng|Dyrk2/4932442E05Rik_chr10_118712062_F Primer3: not found at this Tm N.d. 0.38
mm4_intergenic_Ifng|Dyrk2/4932442E05Rik_chr10_118712062_R Primer3: not found at this Tm N.d. 0.38
mm4_intergenic_Gm25715|N6amt1_chr16_86945902_F TCGTCGGCAGCGTCTGGGAAGTTCCACGTTCATGA 59.5 0.37
mm4_intergenic_Gm25715|N6amt1_chr16_86945902_R GTCTCGTGGGCTCGGGGGGACTGGGTCTGATAAGA 57.8 0.37
mm4_intron_Tmem229b_chr12_78989660_F TCGTCGGCAGCGTCTCTCTTCCCAGGATCAGGTGA 59.6 0.36
mm4_intron_Tmem229b_chr12_78989660_R GTCTCGTGGGCTCGGAGGTACATTGTGACCAGGCA 58.9 0.36
mm4_exon_Vta1_chr10_14684120_F Primer3: not found at this Tm N.d. 0.33
mm4_exon_Vta1_chr10_14684120_R Primer3: not found at this Tm N.d. 0.33
mm3_intergenic_Gm23342|Myt1l_chr12_29326735_F TCGTCGGCAGCGTCCTGATCAAGAGCAGCACGTT 58.5 0.33
mm3_intergenic_Gm23342|Myt1l_chr12_29326735_R GTCTCGTGGGCTCGGGCAGGATATACACTGGCCCC 59.9 0.33
mm4_intergenic_RP24-399L6.2|9230113P08Rik_chr9_35864710_F TCGTCGGCAGCGTCAGTGTCAGGAGCCAACAAGT 59.1 0.32
mm4_intergenic_RP24-399L6.2|9230113P08Rik_chr9_35864710_R GTCTCGTGGGCTCGGTCCAAGATCCCACCTCTTCCT 59.9 0.32
mm4_intergenic_Gm11279|Gm11280_chr13_21904803_F TCGTCGGCAGCGTCTGAAGGATCTCAGGGAGGACT 59.3 0.32
mm4_intergenic_Gm11279|Gm11280_chr13_21904803_R GTCTCGTGGGCTCGGAGCAGTCCTACTTGTCTGCAC 59.9 0.32
mm4_intron_Wdr48_chr9_119906695_F TCGTCGGCAGCGTCGAGTGCTAACTCCCCACCTC 59.4 0.31
mm4_intron_Wdr48_chr9_119906695_R GTCTCGTGGGCTCGGACAACCCAAACCCCAAACAA 58.3 0.31
mm3_intergenic_Gm15696|Senp5_chr16_31975263_F Primer3: not found at this Tm N.d. 0.31
mm3_intergenic_Gm15696|Senp5_chr16_31975263_R Primer3: not found at this Tm N.d. 0.31
mm4_intron_Nrg2_chr18_36030766_F TCGTCGGCAGCGTCATCAGCTGGGGCCCAAATG 60.3 0.31
mm4_intron_Nrg2_chr18_36030766_R GTCTCGTGGGCTCGGTGGCACATCCAGAAACACGT 60.1 0.31
mm3_intron_Mtmr1_chrX_71410481_F Primer3: not found at this Tm N.d. 0.30
mm3_intron_Mtmr1_chrX_71410481_R Primer3: not found at this Tm N.d. 0.30
mm4_intergenic_Ccdc85a|2810471M01Rik_chr11_28633770_F TCGTCGGCAGCGTCCACCTCTGTTGACCTCTGACC 59.9 0.30
mm4_intergenic_Ccdc85a|2810471M01Rik_chr11_28633770_R GTCTCGTGGGCTCGGCCCACTGCACTACCAAGGAG 60.0 0.30
mm4_intergenic_Gm24905|Traf1_chr2_34921611_F Primer3: not found at this Tm N.d. 0.30
mm4_intergenic_Gm24905|Traf1_chr2_34921611_R Primer3: not found at this Tm N.d. 0.30
mm4_intergenic_Kcnq1|KCNQ1OT1_1_chr7_143243207_F Primer3: not found at this Tm N.d. 0.28
mm4_intergenic_Kcnq1|KCNQ1OT1_1_chr7_143243207_R Primer3: not found at this Tm N.d. 0.28
mm4_intergenic_Tcof1|Arsi_chr18_60895046_F Primer3: not found at this Tm N.d. 0.28
mm4_intergenic_Tcof1|Arsi_chr18_60895046_R Primer3: not found at this Tm N.d. 0.28
mm3_intron_Tecta_chr9_42390265_F Primer3: not found at this Tm N.d. 0.28
mm3_intron_Tecta_chr9_42390265_R Primer3: not found at this Tm N.d. 0.28
mm4_intergenic_mmu-mir-6370|Gm22828_chr6_19605289_F TCGTCGGCAGCGTCAACGTGGAATAGGAACATGGT 57.2 0.27
mm4_intergenic_mmu-mir-6370|Gm22828_chr6_19605289_R GTCTCGTGGGCTCGGTCACCATGCATCCACCTCAC 60.0 0.27
mm3_intergenic_Gm26535|Gm24710_chr10_37883313_F TCGTCGGCAGCGTCATAGTCTCTTTTGAGCAGCTGA 57.1 0.27
mm3_intergenic_Gm26535|Gm24710_chr10_37883313_R GTCTCGTGGGCTCGGAGATGTTTCTTCTGGGAATGCA 57.9 0.27
mm4_intron_H2-Q6_chr17_35428828_F TCGTCGGCAGCGTCTATGGCCTGATTGCAGAGGG 59.5 0.26
mm4_intron_H2-Q6_chr17_35428828_R GTCTCGTGGGCTCGGCACAGGGGATTGTGGGAGTC 60.0 0.26
mm4_intron_H2-Q4_chr17_35383823_F TCGTCGGCAGCGTCTATGGCCTGATTGCAGAGGG 59.5 0.26
mm4_intron_H2-Q4_chr17_35383823_R GTCTCGTGGGCTCGGCACAGGGGATTGTGGGAGTC 60.0 0.26
mm4_intron_Mvb12b_chr2_33832707_F Primer3: not found at this Tm N.d. 0.24
mm4_intron_Mvb12b_chr2_33832707_R Primer3: not found at this Tm N.d. 0.24
mm2_intergenic_Alcam|Gm25723_chr16_52596593_F Primer3: not found at this Tm N.d. 0.23
mm2_intergenic_Alcam|Gm25723_chr16_52596593_R Primer3: not found at this Tm N.d. 0.23
mm4_intron_Catsperg2_chr7_29718377_F Primer3: not found at this Tm N.d. 0.23
mm4_intron_Catsperg2_chr7_29718377_R Primer3: not found at this Tm N.d. 0.23
mm4_intergenic_Ednrb|RP24-312G4.2_chr14_104427783_F TCGTCGGCAGCGTCGCGATAAGGCTTCAGCAAAGT 59.2 0.23
mm4_intergenic_Ednrb|RP24-312G4.2_chr14_104427783_R GTCTCGTGGGCTCGGAGTCCTACAGTTATGGTCTGCA 58.5 0.23
mm3_intron_Reep5_chr18_34351996_F Primer3: not found at this Tm N.d. 0.23
mm3_intron_Reep5_chr18_34351996_R Primer3: not found at this Tm N.d. 0.23
mm3_intergenic_Dleu2|Gm26969_chr14_62047863_F Primer3: not found at this Tm N.d. 0.23
mm3_intergenic_Dleu2|Gm26969_chr14_62047863_R Primer3: not found at this Tm N.d. 0.23
mm4_intron_Ncoa7_chr10_30761273_F Primer3: not found at this Tm N.d. 0.23
mm4_intron_Ncoa7_chr10_30761273_R Primer3: not found at this Tm N.d. 0.23
mm4_intergenic_Gm25723|Gm22459_chr16_53257551_F Primer3: not found at this Tm N.d. 0.22
mm4_intergenic_Gm25723|Gm22459_chr16_53257551_R Primer3: not found at this Tm N.d. 0.22
mm3_intergenic_Gm23225|Gm24127_chr14_92148520_F TCGTCGGCAGCGTCGGTATAACCATTTCCTTCTCAATGC 58.2 0.21
mm3_intergenic_Gm23225|Gm24127_chr14_92148520_R GTCTCGTGGGCTCGGAGAAATCAATTCACAGAGCATGAA 57.1 0.21
mm2_intergenic_Gm25562|AI593442_chr9_52405929_F Primer3: not found at this Tm N.d. 0.21
mm2_intergenic_Gm25562|AI593442_chr9_52405929_R Primer3: not found at this Tm N.d. 0.21
mm4_intergenic_4930480K15Rik|Nrxn1_chr17_90870150_F TCGTCGGCAGCGTCCCCCATATAGCCTGCAGTTTCT 59.8 0.20
mm4_intergenic_4930480K15Rik|Nrxn1_chr17_90870150_R GTCTCGTGGGCTCGGAGGCTTGAATCTGCTCTGGT 59.0 0.20
mm4_intron_Edil3_chr13_88860287_F Primer3: not found at this Tm N.d. 0.20
mm4_intron_Edil3_chr13_88860287_R Primer3: not found at this Tm N.d. 0.20
mm4_intergenic_Grik1|Cldn17_chr16_88464276_F Primer3: not found at this Tm N.d. 0.20
mm4_intergenic_Grik1|Cldn17_chr16_88464276_R Primer3: not found at this Tm N.d. 0.20
mm4_intergenic_Rpl31-ps23|Ppp1r3b_chr8_35184128_F Primer3: not found at this Tm N.d. 0.19
mm4_intergenic_Rpl31-ps23|Ppp1r3b_chr8_35184128_R Primer3: not found at this Tm N.d. 0.19
mm4_intergenic_Gm6421|Hcn1_chr13_117399116_F Primer3: not found at this Tm N.d. 0.19
mm4_intergenic_Gm6421|Hcn1_chr13_117399116_R Primer3: not found at this Tm N.d. 0.19
mm4_intron_Zswim2_chr2_83922584_F Primer3: not found at this Tm N.d. 0.19
mm4_intron_Zswim2_chr2_83922584_R Primer3: not found at this Tm N.d. 0.19
mm4_intergenic_Nrg3|Gm10713_chr14_39687638_F TCGTCGGCAGCGTCAGGCTAGCATTGATGGACAGA 58.8 0.19
mm4_intergenic_Nrg3|Gm10713_chr14_39687638_R GTCTCGTGGGCTCGGGCTCACTCTGTGTAGCTCTGT 59.4 0.19
mm3_intron_Man1a_chr10_53957815_F Primer3: not found at this Tm N.d. 0.19
mm3_intron_Man1a_chr10_53957815_R Primer3: not found at this Tm N.d. 0.19
mm3_intergenic_A930039A15Rik|Gm25336_chr1_153920158_F Primer3: not found at this Tm N.d. 0.18
mm3_intergenic_A930039A15Rik|Gm25336_chr1_153920158_R Primer3: not found at this Tm N.d. 0.18
mm2_intron_Vstm2a_chr11_16333000_F Primer3: not found at this Tm N.d. 0.18
mm2_intron_Vstm2a_chr11_16333000_R Primer3: not found at this Tm N.d. 0.18
mm4_intron_Tbc1d8_chr1_39396731_F TCGTCGGCAGCGTCCTTATGCTGCCGGCTTACCT 60.1 0.17
mm4_intron_Tbc1d8_chr1_39396731_R GTCTCGTGGGCTCGGCATGTGGACAGGAACACCGA 59.9 0.17
mm4_intergenic_1700063H04Rik|Rimklb_chr6_122434199_F Primer3: not found at this Tm N.d. 0.17
mm4_intergenic_1700063H04Rik|Rimklb_chr6_122434199_R Primer3: not found at this Tm N.d. 0.17
mm4_intron_Ptprk_chr10_28354232_F TCGTCGGCAGCGTCTCAGCTTCTTGGGTGATGGC 60.3 0.17
mm4_intron_Ptprk_chr10_28354232_R GTCTCGTGGGCTCGGTGAACAACAGTCCTTAATGGGC 58.8 0.17
mm4_intron_Atp8a1_chr5_67742950_F TCGTCGGCAGCGTCTCTTCCCATGGCACTTTCCA 59.2 0.17
mm4_intron_Atp8a1_chr5_67742950_R GTCTCGTGGGCTCGGAGAAAGTGTCCTTCCCAGCC 59.5 0.17
mm4_intron_Pcdh9_chr14_93432676_F Primer3: not found at this Tm N.d. 0.16
mm4_intron_Pcdh9_chr14_93432676_R Primer3: not found at this Tm N.d. 0.16
mm3_intron_Rnf216_chr5_143074093_F Primer3: not found at this Tm N.d. 0.16
mm3_intron_Rnf216_chr5_143074093_R Primer3: not found at this Tm N.d. 0.16
mm4_intergenic_Gm6031|Ube2v2_chr16_15455014_F Primer3: not found at this Tm N.d. 0.15
mm4_intergenic_Gm6031|Ube2v2_chr16_15455014_R Primer3: not found at this Tm N.d. 0.15
mm3_intron_Jak1_chr4_101189210_F TCGTCGGCAGCGTCGGCGAAGAGGTTGTGACAGA 59.9 0.15
mm3_intron_Jak1_chr4_101189210_R GTCTCGTGGGCTCGGTGGGAACAGATGGCCAAGTG 60.2 0.15
mm4_intergenic_Gm22116|Slc35f4_chr14_49521992_F Primer3: not found at this Tm N.d. 0.15
mm4_intergenic_Gm22116|Slc35f4_chr14_49521992_R Primer3: not found at this Tm N.d. 0.15
mm4_intergenic_Gm24298|4921513I03Rik_chr10_120657602_F Primer3: not found at this Tm N.d. 0.15
mm4_intergenic_Gm24298|4921513I03Rik_chr10_120657602_R Primer3: not found at this Tm N.d. 0.15
mm4_intron_Enox1_chr14_77386221_F Primer3: not found at this Tm N.d. 0.15
mm4_intron_Enox1_chr14_77386221_R Primer3: not found at this Tm N.d. 0.15
mm4_intergenic_5S_rRNA|Cops8_chr1_90573178_F TCGTCGGCAGCGTCGCCATCGTGACAGGTGACAT 60.3 0.14
mm4_intergenic_5S_rRNA|Cops8_chr1_90573178_R GTCTCGTGGGCTCGGAGCACACACACCCTTGGATT 59.8 0.14
mm4_exon_Ubr2_chr17_46968382_F Primer3: not found at this Tm N.d. 0.14
mm4_exon_Ubr2_chr17_46968382_R Primer3: not found at this Tm N.d. 0.14
mm4_intergenic_Gm20698/Vmn2r7/Gm26939|Vmn2r7/Gm26939_chr3_64780153_F TCGTCGGCAGCGTCTGAAGGTTTCATAAACTTTAGGAGG 57.0 0.14
mm4_intergenic_Gm20698/Vmn2r7/Gm26939|Vmn2r7/Gm26939_chr3_64780153_R GTCTCGTGGGCTCGGTCATAGAGGACAGAATGTAGCAGA 58.5 0.14
mm4_exon_Bche_chr3_73701266_F Primer3: not found at this Tm N.d. 0.14
mm4_exon_Bche_chr3_73701266_R Primer3: not found at this Tm N.d. 0.14
mm4_intergenic_2810055G20Rik|Gm11146_chr16_77564451_F TCGTCGGCAGCGTCAGTCGCCACTTACATTACTAACA 57.6 0.14
mm4_intergenic_2810055G20Rik|Gm11146_chr16_77564451_R GTCTCGTGGGCTCGGAGACCTTCAATGTGAACGAGAT 57.4 0.14
mm4_intron_Spred2_chr11_19958037_F Primer3: not found at this Tm N.d. 0.14
mm4_intron_Spred2_chr11_19958037_R Primer3: not found at this Tm N.d. 0.14
mm4_intergenic_Gimap3|Tmem176b_chr6_48782100_F TCGTCGGCAGCGTCGGAACACGCATCCCTGTTGA 60.6 0.13
mm4_intergenic_Gimap3|Tmem176b_chr6_48782100_R GTCTCGTGGGCTCGGTGAAAGTGTATGTGATGCATTGGT 59.2 0.13
mm4_intergenic_Gm26250|Gm11819_chr4_13274246_F Primer3: not found at this Tm N.d. 0.13
mm4_intergenic_Gm26250|Gm11819_chr4_13274246_R Primer3: not found at this Tm N.d. 0.13
mm4_intergenic_Ttc8|Rpl30-ps8_chr12_99021720_F Primer3: not found at this Tm N.d. 0.13
mm4_intergenic_Ttc8|Rpl30-ps8_chr12_99021720_R Primer3: not found at this Tm N.d. 0.13
mm4_intergenic_Gm22735|5930412G12Rik_chr5_128457561_F Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm22735|5930412G12Rik_chr5_128457561_R Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Kcnk1|Slc35f3_chr8_126122363_F Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Kcnk1|Slc35f3_chr8_126122363_R Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm24246|Pik3c3_chr18_29785027_F TCGTCGGCAGCGTCGGTAGGTCTTGTCAGGGTGC 60.0 0.12
mm4_intergenic_Gm24246|Pik3c3_chr18_29785027_R GTCTCGTGGGCTCGGTTGTTCAGGCCTCCTTGACC 59.8 0.12
mm4_intron_Xkr4_chr1_3352572_F Primer3: not found at this Tm N.d. 0.12
mm4_intron_Xkr4_chr1_3352572_R Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Slk|Gm16068_chr19_47583728_F Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Slk|Gm16068_chr19_47583728_R Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_mmu-mir-6344|Irs1/Gm9747_chr1_82204713_F TCGTCGGCAGCGTCAGCAGTGGTTGGTAAGTGCC 60.5 0.11
mm4_intergenic_mmu-mir-6344|Irs1/Gm9747_chr1_82204713_R GTCTCGTGGGCTCGGTGTCTCACTAGTTGCCGACA 58.6 0.11
mm4_intergenic_Wdr75|Gm5526_chr1_45841739_F Primer3: not found at this Tm N.d. 0.11
mm4_intergenic_Wdr75|Gm5526_chr1_45841739_R Primer3: not found at this Tm N.d. 0.11
mm4_intergenic_Prkca|Gm11655_chr11_108066049_F Primer3: not found at this Tm N.d. 0.11
mm4_intergenic_Prkca|Gm11655_chr11_108066049_R Primer3: not found at this Tm N.d. 0.11
mm4_intergenic_Gm27034|Rpl36a-ps1_chr14_98954014_F Primer3: not found at this Tm N.d. 0.11
mm4_intergenic_Gm27034|Rpl36a-ps1_chr14_98954014_R Primer3: not found at this Tm N.d. 0.11
mm4_intergenic_Gm8730|Cdh5_chr8_103714788_F Primer3: not found at this Tm N.d. 0.11
mm4_intergenic_Gm8730|Cdh5_chr8_103714788_R Primer3: not found at this Tm N.d. 0.11
mm4_intergenic_Gm5913|Gm7191_chr8_97443335_F Primer3: not found at this Tm N.d. 0.11
mm4_intergenic_Gm5913|Gm7191_chr8_97443335_R Primer3: not found at this Tm N.d. 0.11
mm4_intergenic_Dab1|Gm12719_chr4_103824348_F Primer3: not found at this Tm N.d. 0.11
mm4_intergenic_Dab1|Gm12719_chr4_103824348_R Primer3: not found at this Tm N.d. 0.11
mm4_intron_Tectb_chr19_55185828_F TCGTCGGCAGCGTCTGGGTGTTTCCAAAGCAACA 58.4 0.11
mm4_intron_Tectb_chr19_55185828_R GTCTCGTGGGCTCGGGCTTAGACTCTGGGCTTCCC 59.8 0.11
mm4_intron_Slc6a3_chr13_73541000_F Primer3: not found at this Tm N.d. 0.11
mm4_intron_Slc6a3_chr13_73541000_R Primer3: not found at this Tm N.d. 0.11
mm4_intergenic_Lrrtm1|Ctnna2_chr6_77271533_F TCGTCGGCAGCGTCAGCACTACCTTGGAATTCCTACT 58.8 0.10
mm4_intergenic_Lrrtm1|Ctnna2_chr6_77271533_R GTCTCGTGGGCTCGGTCACGCTCATCTTCTCCCCT 60.3 0.10
mm4_intron_Ppp1r7_chr1_93353112_F TCGTCGGCAGCGTCCCCAGTAAACAGGGCTAGCC 60.1 0.10
mm4_intron_Ppp1r7_chr1_93353112_R GTCTCGTGGGCTCGGTCCCATTCTGACCCATGGTT 58.6 0.10
mm4_intergenic_Atp9b|Sall3_chr18_80941962_F TCGTCGGCAGCGTCGGAGGGGCAGCAATTACTCA 59.7 0.10
mm4_intergenic_Atp9b|Sall3_chr18_80941962_R GTCTCGTGGGCTCGGTGGAATGAATCAGGGCAGGG 59.7 0.10
mm4_intron_Thsd7b_chr1_129565713_F Primer3: not found at this Tm N.d. 0.10
mm4_intron_Thsd7b_chr1_129565713_R Primer3: not found at this Tm N.d. 0.10
mm4_intron_Angpt4_chr2_151921024_F TCGTCGGCAGCGTCTGTGCTGGCAAGTTTCTTCT 57.9 0.10
mm4_intron_Angpt4_chr2_151921024_R GTCTCGTGGGCTCGGCCTGTGGTGGGTGTGTGTAA 59.8 0.10
mm4_intron_Tcf12_chr9_71855392_F Primer3: not found at this Tm N.d. 0.10
mm4_intron_Tcf12_chr9_71855392_R Primer3: not found at this Tm N.d. 0.10
mm4_intergenic_Gm25235|Atf6_chr1_170833044_F TCGTCGGCAGCGTCTGGCCAAAACATCCACACCT 60.1 0.10
mm4_intergenic_Gm25235|Atf6_chr1_170833044_R GTCTCGTGGGCTCGGGTGGGGCTCTCAGGATTCAG 59.8 0.10
mm4_intergenic_Gm14980|Pcdh19_chrX_133451663_F Primer3: not found at this Tm N.d. 0.09
mm4_intergenic_Gm14980|Pcdh19_chrX_133451663_R Primer3: not found at this Tm N.d. 0.09
mm4_intergenic_Adc|Ak2_chr4_128974314_F Primer3: not found at this Tm N.d. 0.09
mm4_intergenic_Adc|Ak2_chr4_128974314_R Primer3: not found at this Tm N.d. 0.09
mm4_intron_Ptpn11_chr5_121186170_F Primer3: not found at this Tm N.d. 0.09
mm4_intron_Ptpn11_chr5_121186170_R Primer3: not found at this Tm N.d. 0.09
mm4_intergenic_Qpct|Cdc42ep3_chr17_79139730_F TCGTCGGCAGCGTCTCTGGCTCTCTGGTAGGCAA 60.2 0.09
mm4_intergenic_Qpct|Cdc42ep3_chr17_79139730_R GTCTCGTGGGCTCGGCCCCACTACATCATCTACCCC 59.0 0.09
mm4_intergenic_Gm25974|Epha4_chr1_77260382_F Primer3: not found at this Tm N.d. 0.09
mm4_intergenic_Gm25974|Epha4_chr1_77260382_R Primer3: not found at this Tm N.d. 0.09
mm4_intergenic_PCA3_1|Prune2_chr19_17070736_F TCGTCGGCAGCGTCTGGAAACAGAGAGGGAAATTACA 57.4 0.09
mm4_intergenic_PCA3_1|Prune2_chr19_17070736_R GTCTCGTGGGCTCGGTTCACTGATAGAATGGCTACCTT 57.0 0.09
mm4_intergenic_Lrp1b|Gm24350_chr2_42753590_F Primer3: not found at this Tm N.d. 0.09
mm4_intergenic_Lrp1b|Gm24350_chr2_42753590_R Primer3: not found at this Tm N.d. 0.09
mm4_intergenic_Gm26235|Gm25392_chr18_19395436_F Primer3: not found at this Tm N.d. 0.09
mm4_intergenic_Gm26235|Gm25392_chr18_19395436_R Primer3: not found at this Tm N.d. 0.09
mm4_intergenic_Gm16013|9230020A06Rik_chr11_71490149_F Primer3: not found at this Tm N.d. 0.09
mm4_intergenic_Gm16013|9230020A06Rik_chr11_71490149_R Primer3: not found at this Tm N.d. 0.09
mm4_intergenic_Gm9603|Gm3373_chr14_5694933_F Primer3: not found at this Tm N.d. 0.09
mm4_intergenic_Gm9603|Gm3373_chr14_5694933_R Primer3: not found at this Tm N.d. 0.09
mm4_intergenic_Gm8356|Gm3629_chr14_6544769_F TCGTCGGCAGCGTCTGTCTTGACTTGTCTGTGACCA 59.5 0.09
mm4_intergenic_Gm8356|Gm3629_chr14_6544769_R GTCTCGTGGGCTCGGCGGAGATCCAGATCAGTGCC 59.9 0.09
mm4_intergenic_Gm8265|Gm9603_chr14_5644424_F Primer3: not found at this Tm N.d. 0.09
mm4_intergenic_Gm8265|Gm9603_chr14_5644424_R Primer3: not found at this Tm N.d. 0.09
mm4_intergenic_Gm6356|Gm10406_chr14_6981004_F TCGTCGGCAGCGTCGTCTGAACTGTGTCAGGCCT 59.6 0.09
mm4_intergenic_Gm6356|Gm10406_chr14_6981004_R GTCTCGTGGGCTCGGTGCCTAGGGTACAGAAAGCC 59.0 0.09
mm4_intergenic_Gm3542|Gm17126_chr14_5342386_F TCGTCGGCAGCGTCTGTCTTGACTTGTCTGTGACCA 59.5 0.09
mm4_intergenic_Gm3542|Gm17126_chr14_5342386_R GTCTCGTGGGCTCGGCGGAGATCCAGATCAGTGCC 59.9 0.09
mm4_intergenic_Gm3500|Gm3194_chr14_5407009_F Primer3: not found at this Tm N.d. 0.09
mm4_intergenic_Gm3500|Gm3194_chr14_5407009_R Primer3: not found at this Tm N.d. 0.09
mm4_intergenic_Gm3373|Gm26557_chr14_5759555_F TCGTCGGCAGCGTCTGTCTTGACTTGTCTGTGACCA 59.5 0.09
mm4_intergenic_Gm3373|Gm26557_chr14_5759555_R GTCTCGTGGGCTCGGCGGAGATCCAGATCAGTGCC 59.9 0.09
mm4_intergenic_Gm3339|Gm3542_chr14_5291910_F Primer3: not found at this Tm N.d. 0.09
mm4_intergenic_Gm3339|Gm3542_chr14_5291910_R Primer3: not found at this Tm N.d. 0.09
mm4_intergenic_Gm17223|Gm10413_chr14_3387992_F TCGTCGGCAGCGTCCGGAGATCCAGATCAGTGCC 59.9 0.09
mm4_intergenic_Gm17223|Gm10413_chr14_3387992_R GTCTCGTGGGCTCGGAGGGGTCTTTGTCTTGACTTGT 59.4 0.09
mm4_intergenic_Gm17221|Gm17188_chr14_3181071_F TCGTCGGCAGCGTCCGGAGATCCAGATCAGTGCC 59.9 0.09
mm4_intergenic_Gm17221|Gm17188_chr14_3181071_R GTCTCGTGGGCTCGGAGGGGTCTTTGTCTTGACTTGT 59.4 0.09
mm4_intergenic_Gm17107|Gm3012_chr14_3627393_F Primer3: not found at this Tm N.d. 0.09
mm4_intergenic_Gm17107|Gm3012_chr14_3627393_R Primer3: not found at this Tm N.d. 0.09
mm4_intergenic_Gm17069|Gm8279_chr14_5124367_F Primer3: not found at this Tm N.d. 0.09
mm4_intergenic_Gm17069|Gm8279_chr14_5124367_R Primer3: not found at this Tm N.d. 0.09
mm4_intergenic_Gm17051|Gm10340_chr14_3122338_F Primer3: not found at this Tm N.d. 0.09
mm4_intergenic_Gm17051|Gm10340_chr14_3122338_R Primer3: not found at this Tm N.d. 0.09
mm4_intergenic_Gm11107|Gm3115_chr14_4072370_F TCGTCGGCAGCGTCCGGAGATCCAGATCAGTGCC 59.9 0.09
mm4_intergenic_Gm11107|Gm3115_chr14_4072370_R GTCTCGTGGGCTCGGCAGGCCTTTTACTAGGGGTCT 58.8 0.09
mm4_intergenic_Gm11105|Gm3138_chr14_4242069_F TCGTCGGCAGCGTCCGGAGATCCAGATCAGTGCC 59.9 0.09
mm4_intergenic_Gm11105|Gm3138_chr14_4242069_R GTCTCGTGGGCTCGGTGTCTTGACTTGTCTGTGACCA 59.5 0.09
mm4_intergenic_Gm10338|Gm10128_chr14_7602707_F Primer3: not found at this Tm N.d. 0.09
mm4_intergenic_Gm10338|Gm10128_chr14_7602707_R Primer3: not found at this Tm N.d. 0.09
mm4_intergenic_Gap|Gm2888_chr14_3023099_F TCGTCGGCAGCGTCCGGAGATCCAGATCAGTGCC 59.9 0.09
mm4_intergenic_Gap|Gm2888_chr14_3023099_R GTCTCGTGGGCTCGGAGGGGTCTTTGTCTTGACTTGT 59.4 0.09
mm4_intergenic_4933406F09Rik|Gm3558_chr14_7513260_F Primer3: not found at this Tm N.d. 0.09
mm4_intergenic_4933406F09Rik|Gm3558_chr14_7513260_R Primer3: not found at this Tm N.d. 0.09
mm4_intergenic_Gm5105|1110002E22Rik/Gm5105_chr3_138061533_F TCGTCGGCAGCGTCGGTTTGCCTTCTGTGAACGG 59.6 0.09
mm4_intergenic_Gm5105|1110002E22Rik/Gm5105_chr3_138061533_R GTCTCGTGGGCTCGGGAAGAACGAAGGCCAGACCA 59.9 0.09
mm3_intergenic_Olfr576|Olfr578_chr7_102976242_F TCGTCGGCAGCGTCAGGGCACACTACAACTCGAA 58.9 0.08
mm3_intergenic_Olfr576|Olfr578_chr7_102976242_R GTCTCGTGGGCTCGGCTCTCCAGTTAAGTCTGCCTCC 59.8 0.08
mm4_intergenic_Gm25258|Hao1_chr2_133670554_F Primer3: not found at this Tm N.d. 0.08
mm4_intergenic_Gm25258|Hao1_chr2_133670554_R Primer3: not found at this Tm N.d. 0.08
mm4_intergenic_Gm5641|Gm26678_chr3_54579127_F TCGTCGGCAGCGTCTGTTGTTTCTTCTGTGTTCCTACT 58.2 0.08
mm4_intergenic_Gm5641|Gm26678_chr3_54579127_R GTCTCGTGGGCTCGGTCACAAATATCACTTACCAACCTGA 58.2 0.08
mm4_intergenic_Gm25434|Rergl_chr6_139466643_F TCGTCGGCAGCGTCCCAAAGACCACTTGCATGCA 59.3 0.08
mm4_intergenic_Gm25434|Rergl_chr6_139466643_R GTCTCGTGGGCTCGGGCAAAGACTTGGGAAGGGTTC 59.3 0.08
mm4_intergenic_Gm27037|Gm23859_chr18_89954897_F Primer3: not found at this Tm N.d. 0.08
mm4_intergenic_Gm27037|Gm23859_chr18_89954897_R Primer3: not found at this Tm N.d. 0.08
mm4_intron_Ncam1_chr9_49697711_F TCGTCGGCAGCGTCGTCTGTGCTCTGACCCCTTC 60.0 0.08
mm4_intron_Ncam1_chr9_49697711_R GTCTCGTGGGCTCGGTGGGGATATTCTTATGAAGTAGCC 57.6 0.08
mm4_intergenic_Megf9|Gm11227_chr4_70721682_F TCGTCGGCAGCGTCGGGCACTTGTATTCTCCCACA 59.9 0.08
mm4_intergenic_Megf9|Gm11227_chr4_70721682_R GTCTCGTGGGCTCGGAGCATCTGAAAATGTGTTGCCT 59.1 0.08
mm4_intron_Gpr39_chr1_125768322_F Primer3: not found at this Tm N.d. 0.07
mm4_intron_Gpr39_chr1_125768322_R Primer3: not found at this Tm N.d. 0.07
mm4_intron_Khdrbs2_chr1_32406170_F Primer3: not found at this Tm N.d. 0.07
mm4_intron_Khdrbs2_chr1_32406170_R Primer3: not found at this Tm N.d. 0.07
mm3_intron_Nell1_chr7_50853281_F Primer3: not found at this Tm N.d. 0.07
mm3_intron_Nell1_chr7_50853281_R Primer3: not found at this Tm N.d. 0.07
mm4_intergenic_Smg6|Gm12333/Smg6_chr11_75030015_F Primer3: not found at this Tm N.d. 0.06
mm4_intergenic_Smg6|Gm12333/Smg6_chr11_75030015_R Primer3: not found at this Tm N.d. 0.06
mm4_intergenic_1700061I17Rik|D3Bwg0562e/Gm22408_chr3_117084777_F Primer3: not found at this Tm N.d. 0.06
mm4_intergenic_1700061I17Rik|D3Bwg0562e/Gm22408_chr3_117084777_R Primer3: not found at this Tm N.d. 0.06
mm4_intron_5430435G22Rik_chr1_131695319_F Primer3: not found at this Tm N.d. 0.06
mm4_intron_5430435G22Rik_chr1_131695319_R Primer3: not found at this Tm N.d. 0.06
mm4_intergenic_Cabp5|Bsph1_chr7_13427790_F Primer3: not found at this Tm N.d. 0.06
mm4_intergenic_Cabp5|Bsph1_chr7_13427790_R Primer3: not found at this Tm N.d. 0.06
mm4_intergenic_Zfp451|Gm15455_chr1_33831036_F Primer3: not found at this Tm N.d. 0.06
mm4_intergenic_Zfp451|Gm15455_chr1_33831036_R Primer3: not found at this Tm N.d. 0.06
mm4_intergenic_Glra2|Gm15236_chrX_165520262_F Primer3: not found at this Tm N.d. 0.06
mm4_intergenic_Glra2|Gm15236_chrX_165520262_R Primer3: not found at this Tm N.d. 0.06
mm4_intron_Spon1_chr7_113827975_F TCGTCGGCAGCGTCAGGAGCTGAAAGATGCCACA 59.3 0.06
mm4_intron_Spon1_chr7_113827975_R GTCTCGTGGGCTCGGCGGGCTCCCTTTGTTGACTT 60.5 0.06
mm4_intergenic_Mir128-1|R3hdm1_chr1_128210609_F Primer3: not found at this Tm N.d. 0.06
mm4_intergenic_Mir128-1|R3hdm1_chr1_128210609_R Primer3: not found at this Tm N.d. 0.06
mm4_intergenic_Ap3b1|Tbca_chr13_94653125_F Primer3: not found at this Tm N.d. 0.06
mm4_intergenic_Ap3b1|Tbca_chr13_94653125_R Primer3: not found at this Tm N.d. 0.06
mm4_intergenic_Gm12026|Gm12027_chr11_19454226_F TCGTCGGCAGCGTCGGGACTTCATGAGTGAGGGC 60.1 0.06
mm4_intergenic_Gm12026|Gm12027_chr11_19454226_R GTCTCGTGGGCTCGGCCGGTGCTCACTAACCACTT 59.9 0.06
mm4_intergenic_Gm22327|Zfand3_chr17_29985544_F TCGTCGGCAGCGTCTTCGAATCCACGACCTCACC 59.7 0.05
mm4_intergenic_Gm22327|Zfand3_chr17_29985544_R GTCTCGTGGGCTCGGCAGAAGAAAGCCTGGCCAGA 59.9 0.05
mm3_intron_Ank2_chr3_127480376_F Primer3: not found at this Tm N.d. 0.05
mm3_intron_Ank2_chr3_127480376_R Primer3: not found at this Tm N.d. 0.05
mm3_exon_Mrpl32_chr13_14610685_F Primer3: not found at this Tm N.d. 0.05
mm3_exon_Mrpl32_chr13_14610685_R Primer3: not found at this Tm N.d. 0.05
mm3_exon_Gm6578_chr6_12099905_F TCGTCGGCAGCGTCTTTGTCGTCTCCTGGCGTAC 60.0 0.05
mm3_exon_Gm6578_chr6_12099905_R GTCTCGTGGGCTCGGCCGATGTAGGAGAGGAAACCC 59.5 0.05
mm3_exon_Cntn3_chr6_102164003_F TCGTCGGCAGCGTCGCCATGTCTTCCACAGTCCT 59.6 0.05
mm3_exon_Cntn3_chr6_102164003_R GTCTCGTGGGCTCGGCAGGCAGCCTTTTCCATTCC 59.4 0.05
mm4_intergenic_Gm22937|1700049L16Rik_chr10_71952383_F Primer3: not found at this Tm N.d. 0.05
mm4_intergenic_Gm22937|1700049L16Rik_chr10_71952383_R Primer3: not found at this Tm N.d. 0.05
mm3_intron_Col17a1_chr19_47684306_F TCGTCGGCAGCGTCATGTGTGTGGGAGGAGGGAT 60.2 0.05
mm3_intron_Col17a1_chr19_47684306_R GTCTCGTGGGCTCGGTCATGTGGAGGGCAGGGATA 60.0 0.05
mm4_intron_Ahdc1_chr4_133050869_F TCGTCGGCAGCGTCGGGTGGGATGCCATGTCTAG 59.8 0.05
mm4_intron_Ahdc1_chr4_133050869_R GTCTCGTGGGCTCGGCGCCATGGGAGAAGTCTCTT 59.4 0.05
mm4_intron_A230071N21Rik_chr7_112527649_F TCGTCGGCAGCGTCGTGGTCATGTCATGGAGCCA 60.0 0.05
mm4_intron_A230071N21Rik_chr7_112527649_R GTCTCGTGGGCTCGGACAGAGCAGTAAGGAACAGACT 58.7 0.05
mm4_intron_Gm15155_chrX_156186531_F Primer3: not found at this Tm N.d. 0.05
mm4_intron_Gm15155_chrX_156186531_R Primer3: not found at this Tm N.d. 0.05
mm4_intron_4930556M19Rik_chr15_10736219_F TCGTCGGCAGCGTCCCCAACGAAGGATCTGGTTACA 60.0 0.05
mm4_intron_4930556M19Rik_chr15_10736219_R GTCTCGTGGGCTCGGAGGGATGCGAAGTGTTTGGA 59.6 0.05
mm4_intergenic_Gm12702|Ror1_chr4_100070538_F Primer3: not found at this Tm N.d. 0.05
mm4_intergenic_Gm12702|Ror1_chr4_100070538_R Primer3: not found at this Tm N.d. 0.05
mm4_intergenic_Gm25923|Gm25564_chr12_28325579_F Primer3: not found at this Tm N.d. 0.05
mm4_intergenic_Gm25923|Gm25564_chr12_28325579_R Primer3: not found at this Tm N.d. 0.05
mm3_intergenic_Gm23745|Gm24177_chr4_53979284_F Primer3: not found at this Tm N.d. 0.05
mm3_intergenic_Gm23745|Gm24177_chr4_53979284_R Primer3: not found at this Tm N.d. 0.05
mm4_intergenic_Gm25602|Tbc1d32_chr10_55057347_F Primer3: not found at this Tm N.d. 0.05
mm4_intergenic_Gm25602|Tbc1d32_chr10_55057347_R Primer3: not found at this Tm N.d. 0.05
mm4_intergenic_Gm22183|mmu-mir-378d_chr10_126100846_F Primer3: not found at this Tm N.d. 0.04
mm4_intergenic_Gm22183|mmu-mir-378d_chr10_126100846_R Primer3: not found at this Tm N.d. 0.04
mm3_intergenic_AC166333.1|Scg2_chr1_79153746_F Primer3: not found at this Tm N.d. 0.04
mm3_intergenic_AC166333.1|Scg2_chr1_79153746_R Primer3: not found at this Tm N.d. 0.04
mm4_intron_Disp1_chr1_183117541_F Primer3: not found at this Tm N.d. 0.04
mm4_intron_Disp1_chr1_183117541_R Primer3: not found at this Tm N.d. 0.04
mm3_intergenic_Robo1|Gm25699_chr16_73389541_F TCGTCGGCAGCGTCTGCTTTCTGATGAGGTGAACTGA 59.9 0.04
mm3_intergenic_Robo1|Gm25699_chr16_73389541_R GTCTCGTGGGCTCGGACAATACACTACCTGGCTCTACT 58.3 0.04
mm4_intergenic_Gm8805|Crygf_chr1_65725868_F TCGTCGGCAGCGTCTGTGCATTGAGGAAGTCTCTCT 59.0 0.04
mm4_intergenic_Gm8805|Crygf_chr1_65725868_R GTCTCGTGGGCTCGGAAGTAAACACTAACCTGTAGCCA 57.4 0.04
mm4_intron_Diap3_chr14_86726916_F TCGTCGGCAGCGTCTGTCAAGATTGGAGAAGTGAAGA 57.4 0.04
mm4_intron_Diap3_chr14_86726916_R GTCTCGTGGGCTCGGCCCCTTTCCTAGAGTCCTCTCA 60.0 0.04
mm4_intergenic_Ces5a|Gm26843_chr8_93602267_F Primer3: not found at this Tm N.d. 0.04
mm4_intergenic_Ces5a|Gm26843_chr8_93602267_R Primer3: not found at this Tm N.d. 0.04
mm4_intergenic_Poteg|Gm1698_chr8_27548647_F TCGTCGGCAGCGTCGCTTGTCTTGAAGGGCATGC 60.1 0.04
mm4_intergenic_Poteg|Gm1698_chr8_27548647_R GTCTCGTGGGCTCGGAGGAGTTGCACAGAGATTTCCT 59.3 0.04
mm4_intron_Dcc_chr18_71898460_F Primer3: not found at this Tm N.d. 0.04
mm4_intron_Dcc_chr18_71898460_R Primer3: not found at this Tm N.d. 0.04
mm4_intron_Lama2_chr10_27281504_F Primer3: not found at this Tm N.d. 0.04
mm4_intron_Lama2_chr10_27281504_R Primer3: not found at this Tm N.d. 0.04
mm4_intergenic_Bbs9|Bmper_chr9_23145779_F TCGTCGGCAGCGTCGGGGCATAGGAATGACACACT 59.7 0.04
mm4_intergenic_Bbs9|Bmper_chr9_23145779_R GTCTCGTGGGCTCGGCCTCCAAAATGAACATGGCCC 59.7 0.04
mm4_exon_Ap2a2_chr7_141631708_F TCGTCGGCAGCGTCGCCACTGGCTCAATCTCAGA 59.7 0.04
mm4_exon_Ap2a2_chr7_141631708_R GTCTCGTGGGCTCGGGACACTGCCTAGCCCATGAG 60.1 0.04
mm4_exon_Matn4_chr2_164389783_F TCGTCGGCAGCGTCCTCAGAGATCCCTTCACCCG 59.2 0.04
mm4_exon_Matn4_chr2_164389783_R GTCTCGTGGGCTCGGGAAAGCCTCGTGGAGTTCCA 59.9 0.04
mm4_intergenic_Npsr1|Gm10701_chr9_24332976_F Primer3: not found at this Tm N.d. 0.04
mm4_intergenic_Npsr1|Gm10701_chr9_24332976_R Primer3: not found at this Tm N.d. 0.04
mm3_intergenic_Gm11850|Gm11849_chr4_17063909_F Primer3: not found at this Tm N.d. 0.03
mm3_intergenic_Gm11850|Gm11849_chr4_17063909_R Primer3: not found at this Tm N.d. 0.03
mm4_intergenic_Gm12468|Gm23745_chr4_53889063_F Primer3: not found at this Tm N.d. 0.03
mm4_intergenic_Gm12468|Gm23745_chr4_53889063_R Primer3: not found at this Tm N.d. 0.03
mm4_intergenic_Gm23339|Gm25570_chr6_105248042_F Primer3: not found at this Tm N.d. 0.03
mm4_intergenic_Gm23339|Gm25570_chr6_105248042_R Primer3: not found at this Tm N.d. 0.03
mm4_intergenic_Gm7091|Vsig1_chrX_140871959_F Primer3: not found at this Tm N.d. 0.03
mm4_intergenic_Gm7091|Vsig1_chrX_140871959_R Primer3: not found at this Tm N.d. 0.03
mm3_intergenic_Gm25985|Gm6404_chr13_115440290_F Primer3: not found at this Tm N.d. 0.03
mm3_intergenic_Gm25985|Gm6404_chr13_115440290_R Primer3: not found at this Tm N.d. 0.03
mm4_intergenic_Trhr|Gm24903_chr15_44264699_F Primer3: not found at this Tm N.d. 0.03
mm4_intergenic_Trhr|Gm24903_chr15_44264699_R Primer3: not found at this Tm N.d. 0.03
mm4_intergenic_Prpf19|Zp1_chr19_10910417_F TCGTCGGCAGCGTCAATAACAGCCACAAAGGGCA 57.9 0.03
mm4_intergenic_Prpf19|Zp1_chr19_10910417_R GTCTCGTGGGCTCGGCGGGGTTCCTTCATGCTTCA 60.3 0.03
mm4_intergenic_Ctnna3|Gm22829_chr10_65677992_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Ctnna3|Gm22829_chr10_65677992_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Msr1|Fgf20_chr8_39671356_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Msr1|Fgf20_chr8_39671356_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Nrp1|Gm26002_chr8_128650858_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Nrp1|Gm26002_chr8_128650858_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Crp|Gm24817_chr1_172757952_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Crp|Gm24817_chr1_172757952_R Primer3: not found at this Tm N.d. 0.02
mm4_intron_Gas7_chr11_67516235_F TCGTCGGCAGCGTCAGCCTTCTGTGGAATCGCTC 60.1 0.02
mm4_intron_Gas7_chr11_67516235_R GTCTCGTGGGCTCGGGCTGCATGAACCTGAGTCCA 60.3 0.02
mm4_intergenic_5S_rRNA|Gm10649_chr8_76627640_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_5S_rRNA|Gm10649_chr8_76627640_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Gm23203|Gm23447_chr17_59657621_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Gm23203|Gm23447_chr17_59657621_R Primer3: not found at this Tm N.d. 0.02
mm4_intron_Tbc1d4_chr14_101540221_F Primer3: not found at this Tm N.d. 0.02
mm4_intron_Tbc1d4_chr14_101540221_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Gm5976|Tmem194b_chr1_52547245_F TCGTCGGCAGCGTCAGGGCTTGGGAGAGTTCTACT 59.9 0.02
mm4_intergenic_Gm5976|Tmem194b_chr1_52547245_R GTCTCGTGGGCTCGGGCCATGGCTGAAGAGGTCTT 60.0 0.02
mm4_intergenic_Gm14631|Gm25023_chrX_57783115_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Gm14631|Gm25023_chrX_57783115_R Primer3: not found at this Tm N.d. 0.02
mm3_intergenic_Zic4|Gm26101_chr9_91592804_F Primer3: not found at this Tm N.d. 0.02
mm3_intergenic_Zic4|Gm26101_chr9_91592804_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Gm23526|Grik4_chr9_42933357_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Gm23526|Grik4_chr9_42933357_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Gm16477|Gpr26_chr7_131896789_F TCGTCGGCAGCGTCCCCATAGTGTCATGACCATGGT 59.8 0.02
mm4_intergenic_Gm16477|Gpr26_chr7_131896789_R GTCTCGTGGGCTCGGTGTTGCCAACAAAATCACCAA 57.3 0.02
mm4_intergenic_Gm14809|Gm14812_chrX_99427019_F Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Gm14809|Gm14812_chrX_99427019_R Primer3: not found at this Tm N.d. 0.01
mm3_intergenic_Nrcam|Stxbp6_chr12_44638821_F TCGTCGGCAGCGTCTTGGCAGGGTTCAGCTCATT 59.8 0.01
mm3_intergenic_Nrcam|Stxbp6_chr12_44638821_R GTCTCGTGGGCTCGGGTCACAGAATCAGATCCTTCGC 59.1 0.01
mm4_intergenic_Ttc32|Gm7099_chr12_9192537_F TCGTCGGCAGCGTCGCACCGTGGACTTTGGGTAA 60.5 0.01
mm4_intergenic_Ttc32|Gm7099_chr12_9192537_R GTCTCGTGGGCTCGGTCCCTACATCCAGGTCCCAG 60.0 0.01
mm4_intergenic_Gm22289|Gm22373_chr7_59917578_F Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Gm22289|Gm22373_chr7_59917578_R Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Gm23464|S100a2_chr3_90562004_F TCGTCGGCAGCGTCGCCACTGCCTGGTATGTTCA 60.3 0.01
mm4_intergenic_Gm23464|S100a2_chr3_90562004_R GTCTCGTGGGCTCGGATCACAGGTGCTTGCATCCA 59.9 0.01
mm3_intergenic_Lmo1|Stk33_chr7_109211235_F Primer3: not found at this Tm N.d. 0.01
mm3_intergenic_Lmo1|Stk33_chr7_109211235_R Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Rpl21-ps5|Gm24508_chr16_83614777_F Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Rpl21-ps5|Gm24508_chr16_83614777_R Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Prickle1|Adamts20_chr15_94166831_F Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Prickle1|Adamts20_chr15_94166831_R Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Gm7488|Tbl1xr1_chr3_21860778_F Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Gm7488|Tbl1xr1_chr3_21860778_R Primer3: not found at this Tm N.d. 0.01
mm3_intergenic_Arid5b|1700040L02Rik_chr10_68401693_F TCGTCGGCAGCGTCGTTTGCTTGCGTTGGTAGGG 60.0 0.01
mm3_intergenic_Arid5b|1700040L02Rik_chr10_68401693_R GTCTCGTGGGCTCGGTCAGGAATGCAGCAAGGGAG 60.0 0.01
mm4_intron_Tfdp2_chr9_96299120_F Primer3: not found at this Tm N.d. 0.01
mm4_intron_Tfdp2_chr9_96299120_R Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Gm5356|Gm26331_chr8_89245838_F Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Gm5356|Gm26331_chr8_89245838_R Primer3: not found at this Tm N.d. 0.01
mm4_intron_Nrxn3_chr12_90145376_F TCGTCGGCAGCGTCTGGAAGATCCACCCGTCTAT 57.5 0.01
mm4_intron_Nrxn3_chr12_90145376_R GTCTCGTGGGCTCGGACAAGTGTCATTAGCAGACAGGT 59.9 0.01
mm4_intron_Atp8a2_chr14_59672141_F Primer3: not found at this Tm N.d. 0.01
mm4_intron_Atp8a2_chr14_59672141_R Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Gm22227|Zfp703_chr8_26501939_F TCGTCGGCAGCGTCAATAGACGCCGCTGACCTTC 60.1 0.01
mm4_intergenic_Gm22227|Zfp703_chr8_26501939_R GTCTCGTGGGCTCGGAGATTCTGTTCTGCACCGCA 59.9 0.01
mm4_intergenic_Fars2|Gm26395_chr13_36617940_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Fars2|Gm26395_chr13_36617940_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm7936|Gm24396_chr18_30535689_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm7936|Gm24396_chr18_30535689_R Primer3: not found at this Tm N.d. 0.00
mm4_intron_Rp1l1_chr14_64019654_F TCGTCGGCAGCGTCTGGTACTGGGGACATGGTGA 60.1 0.00
mm4_intron_Rp1l1_chr14_64019654_R GTCTCGTGGGCTCGGCCCTTCAGTCGGCATGTTCT 60.0 0.00
mm4_intron_Dcc_chr18_71881704_F Primer3: not found at this Tm N.d. 0.00
mm4_intron_Dcc_chr18_71881704_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Olfr531|Olfr532_chr7_140404768_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Olfr531|Olfr532_chr7_140404768_R Primer3: not found at this Tm N.d. 0.00
mm4_intron_Cdh13_chr8_118875049_F TCGTCGGCAGCGTCATCTTTCCCAGGCAGGCATC 60.1 0.00
mm4_intron_Cdh13_chr8_118875049_R GTCTCGTGGGCTCGGACCTGCTCCAAAGATCCCAA 58.9 0.00
mm4_intron_Fam175b_chr7_132861899_F Primer3: not found at this Tm N.d. 0.00
mm4_intron_Fam175b_chr7_132861899_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Galnt9|Noc4l_chr5_110623643_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Galnt9|Noc4l_chr5_110623643_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm14519|Gm14518_chrX_15400845_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm14519|Gm14518_chrX_15400845_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Rpn1/mmu-mir-6376|Gata2_chr6_88106392_F TCGTCGGCAGCGTCGTTCCAGTATTACCGCGCCT 60.1 0.00
mm4_intergenic_Rpn1/mmu-mir-6376|Gata2_chr6_88106392_R GTCTCGTGGGCTCGGCATGATGCACTGTTCCAGCC 59.5 0.00
mm4_intron_Flt1_chr5_147631485_F TCGTCGGCAGCGTCGGCAGCTTAGGGGAGTGAAA 59.6 0.00
mm4_intron_Flt1_chr5_147631485_R GTCTCGTGGGCTCGGGGCCTAACACTGCCCTCATT 60.0 0.00
mm4_intergenic_Tmem37|Dbi_chr1_120102364_F TCGTCGGCAGCGTCCCACACTCCACACACAGAGG 60.2 0.00
mm4_intergenic_Tmem37|Dbi_chr1_120102364_R GTCTCGTGGGCTCGGGGTAATGGGTGACTCTAGCAGT 59.2 0.00
mm4_intergenic_Adamts3|Eif5al3-ps_chr5_90071267_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Adamts3|Eif5al3-ps_chr5_90071267_R Primer3: not found at this Tm N.d. 0.00
mm4_intron_Slco5a1_chr1_12885247_F Primer3: not found at this Tm N.d. 0.00
mm4_intron_Slco5a1_chr1_12885247_R Primer3: not found at this Tm N.d. 0.00
mm4_intron_Sh3yl1_chr12_30929979_F Primer3: not found at this Tm N.d. 0.00
mm4_intron_Sh3yl1_chr12_30929979_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm11528|Phb_chr11_95655123_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm11528|Phb_chr11_95655123_R Primer3: not found at this Tm N.d. 0.00
mm4_intron_Mrgpra3_chr7_47592236_F TCGTCGGCAGCGTCATAGAGGCTGGCAGAGGACA 60.0 0.00
mm4_intron_Mrgpra3_chr7_47592236_R GTCTCGTGGGCTCGGTGGCTTTTCTCCCATCCACA 59.2 0.00
mm4_intergenic_Mrgpra2b|Mrgpra3_chr7_47558442_F TCGTCGGCAGCGTCATAGAGGCTGGCAGAGGACA 60.0 0.00
mm4_intergenic_Mrgpra2b|Mrgpra3_chr7_47558442_R GTCTCGTGGGCTCGGTGGCTTTTCTCCCATCCACA 59.2 0.00
mm4_intergenic_Gm23061|Ostf1_chr19_17987943_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm23061|Ostf1_chr19_17987943_R Primer3: not found at this Tm N.d. 0.00
mm4_intron_Efna5_chr17_62659063_F TCGTCGGCAGCGTCCAGGTTAAGAAGGGAAAGGAGACA 59.9 0.00
mm4_intron_Efna5_chr17_62659063_R GTCTCGTGGGCTCGGGACTGATAGGCACACCCAGC 60.4 0.00
mm4_exon_Alg3_chr16_20606516_F Primer3: not found at this Tm N.d. 0.00
mm4_exon_Alg3_chr16_20606516_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm24221|Sstr4_chr2_148329941_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm24221|Sstr4_chr2_148329941_R Primer3: not found at this Tm N.d. 0.00
mm4_intron_Sec24d_chr3_123314982_F Primer3: not found at this Tm N.d. 0.00
mm4_intron_Sec24d_chr3_123314982_R Primer3: not found at this Tm N.d. 0.00
mm4_intron_Rbms3_chr9_116791965_F Primer3: not found at this Tm N.d. 0.00
mm4_intron_Rbms3_chr9_116791965_R Primer3: not found at this Tm N.d. 0.00
mm4_intron_Ralyl_chr3_14109393_F TCGTCGGCAGCGTCGCAATTGGCAGTTTAATCCAACAC 59.8 0.00
mm4_intron_Ralyl_chr3_14109393_R GTCTCGTGGGCTCGGACATCGGGAAACATCCATGACA 60.0 0.00
mm4_intron_Rab3ip_chr10_116939490_F TCGTCGGCAGCGTCGGGGTCGTTAGCCATAGCAA 59.8 0.00
mm4_intron_Rab3ip_chr10_116939490_R GTCTCGTGGGCTCGGGCTTTATATGGATTGTTTTACAGCC 57.0 0.00
mm4_intron_Psma8_chr18_14710475_F Primer3: not found at this Tm N.d. 0.00
mm4_intron_Psma8_chr18_14710475_R Primer3: not found at this Tm N.d. 0.00
mm4_intron_Nsmce2_chr15_59517069_F TCGTCGGCAGCGTCGACTTCCTGGGCCTGTCATG 60.3 0.00
mm4_intron_Nsmce2_chr15_59517069_R GTCTCGTGGGCTCGGTCAGGGTGCAAGTGCTGAAA 60.1 0.00
mm4_intron_Col14a1_chr15_55465005_F TCGTCGGCAGCGTCGCCTCGAGTTTTCATGCCAC 59.8 0.00
mm4_intron_Col14a1_chr15_55465005_R GTCTCGTGGGCTCGGGTCATGAATCTCCTTTTGTGTCTGT 59.5 0.00
mm4_intron_Cntnap2_chr6_45096266_F Primer3: not found at this Tm N.d. 0.00
mm4_intron_Cntnap2_chr6_45096266_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Veph1|Shox2_chr3_66935869_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Veph1|Shox2_chr3_66935869_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Unc5d|4933433F19Rik_chr8_29752915_F TCGTCGGCAGCGTCGCCGGGATAATGGAGTCTGG 59.9 0.00
mm4_intergenic_Unc5d|4933433F19Rik_chr8_29752915_R GTCTCGTGGGCTCGGCCCTGTTGGATGAAGACAGCA 60.2 0.00
mm4_intergenic_Tktl2|Gm16330_chr8_66570005_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Tktl2|Gm16330_chr8_66570005_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Slc19a3|Ccl20_chr1_83104736_F TCGTCGGCAGCGTCAGACAGGTCTTTTGTTTGTTTGT 57.2 0.00
mm4_intergenic_Slc19a3|Ccl20_chr1_83104736_R GTCTCGTGGGCTCGGTTGTGCATGCTTAGGCCAGA 59.9 0.00
mm4_intergenic_Siglec1|Hspa12b_chr2_131106112_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Siglec1|Hspa12b_chr2_131106112_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Setbp1|Gm25824_chr18_79170828_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Setbp1|Gm25824_chr18_79170828_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Serp2|Lacc1_chr14_76615262_F TCGTCGGCAGCGTCACAACTGCCAAACCATTGCC 59.8 0.00
mm4_intergenic_Serp2|Lacc1_chr14_76615262_R GTCTCGTGGGCTCGGGATCCGCCCATCTACCTGTG 59.9 0.00
mm4_intergenic_Rps24-ps2|Gm8775_chr3_3988540_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Rps24-ps2|Gm8775_chr3_3988540_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Pard3|Gm6921_chr8_127551533_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Pard3|Gm6921_chr8_127551533_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Pacrg|A230009B12Rik_chr17_10439142_F TCGTCGGCAGCGTCGGCCCTGGTCACACATAACA 59.9 0.00
mm4_intergenic_Pacrg|A230009B12Rik_chr17_10439142_R GTCTCGTGGGCTCGGAAGGGTCTCTCTGAAGCCCA 60.1 0.00
mm4_intergenic_Igkv10-95|Igkv10-94_chr6_68687189_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Igkv10-95|Igkv10-94_chr6_68687189_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm833|Gm25779_chr4_152922320_F TCGTCGGCAGCGTCCTGTCAAGGAGCTGAGCACA 59.9 0.00
mm4_intergenic_Gm833|Gm25779_chr4_152922320_R GTCTCGTGGGCTCGGTCTCTGAAATAAACCTTAGTGGCCT 59.7 0.00
mm4_intergenic_Gm26326|Dnajb9_chr12_43896987_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm26326|Dnajb9_chr12_43896987_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm25898|Gm13501_chr2_53297458_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm25898|Gm13501_chr2_53297458_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm24382|Mbnl1_chr3_60203133_F TCGTCGGCAGCGTCACAGAGAGAGTGTGCTGAACA 58.9 0.00
mm4_intergenic_Gm24382|Mbnl1_chr3_60203133_R GTCTCGTGGGCTCGGGCTGATTGGTTCTTTCCTCTGC 59.8 0.00
mm4_intergenic_Gm24063|Rps15a_chr7_117889287_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm24063|Rps15a_chr7_117889287_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm23924|Gm22254_chr6_74497492_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm23924|Gm22254_chr6_74497492_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm22308|Cbfa2t2-ps1_chr5_59857937_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm22308|Cbfa2t2-ps1_chr5_59857937_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm17173|Txndc16_chr14_45128826_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm17173|Txndc16_chr14_45128826_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm14975|Gm4916_chrX_128353260_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm14975|Gm4916_chrX_128353260_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm14914|Gm14917_chrX_117788062_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm14914|Gm14917_chrX_117788062_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm13988|Sema6d_chr2_123803202_F TCGTCGGCAGCGTCCACCTGTGAATGGTGCAAGT 58.6 0.00
mm4_intergenic_Gm13988|Sema6d_chr2_123803202_R GTCTCGTGGGCTCGGAGCCATTATTACCTTGTGTAAGTGA 58.2 0.00
mm4_intergenic_Gm10287|Gm26468_chr3_149289830_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm10287|Gm26468_chr3_149289830_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Fbxo15|Gm25453_chr18_85297064_F TCGTCGGCAGCGTCGCCTCCCATGCCAAAATACC 59.2 0.00
mm4_intergenic_Fbxo15|Gm25453_chr18_85297064_R GTCTCGTGGGCTCGGCGTTGTTTGAGACTCACTGGC 59.7 0.00
mm4_intergenic_Fam174a|Gm15427_chr1_95353720_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Fam174a|Gm15427_chr1_95353720_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Eda2r|Gm9062_chrX_97685398_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Eda2r|Gm9062_chrX_97685398_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Casp12|Pdgfd_chr9_5795438_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Casp12|Pdgfd_chr9_5795438_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Camsap2|9230116N13Rik_chr1_136383615_F TCGTCGGCAGCGTCACACAGAGATCCCAGGACTT 57.6 0.00
mm4_intergenic_Camsap2|9230116N13Rik_chr1_136383615_R GTCTCGTGGGCTCGGACTGTCTACTTAAAGCCACACACA 60.1 0.00
mm4_intergenic_Arrdc4|Gm23795_chr7_68822638_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Arrdc4|Gm23795_chr7_68822638_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_6330403L08Rik/Pdgfa|Prkar1b_chr5_139011242_F TCGTCGGCAGCGTCCCACTGGAGCCATCAACTGT 59.9 0.00
mm4_intergenic_6330403L08Rik/Pdgfa|Prkar1b_chr5_139011242_R GTCTCGTGGGCTCGGAAACTGACAGGCCAAGGAGG 59.8 0.00
mm4_intergenic_5S_rRNA|Auts2_chr5_131613260_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_5S_rRNA|Auts2_chr5_131613260_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_4631405K08Rik|Plxna2_chr1_193644601_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_4631405K08Rik|Plxna2_chr1_193644601_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_1700019M22Rik|Gm26480_chr12_96169874_F TCGTCGGCAGCGTCACCAGGAGATCCCAAAAGTAGA 58.1 0.00
mm4_intergenic_1700019M22Rik|Gm26480_chr12_96169874_R GTCTCGTGGGCTCGGTGGAGACATGAGCTGAGTTTGA 59.3 0.00
mm4_exon_Spc25_chr2_69199492_F TCGTCGGCAGCGTCGCCTGCACGTGATCTTAGGA 59.8 0.00
mm4_exon_Spc25_chr2_69199492_R GTCTCGTGGGCTCGGTGGGAGGAGCCTTTCTCAGA 59.8 0.00
mm4_exon_Ntrk2_chr13_58934623_F TCGTCGGCAGCGTCTCCCTGGCTTTCTTTGGTCC 59.8 0.00
mm4_exon_Ntrk2_chr13_58934623_R GTCTCGTGGGCTCGGCCTGTCAGAGAGCACAGACC 59.7 0.00
mm3_intergenic_Pdk3|Supt20-ps_chrX_93912605_F TCGTCGGCAGCGTCGTTCTCTTCCCATGGCCCAA 59.9 0.00
mm3_intergenic_Pdk3|Supt20-ps_chrX_93912605_R GTCTCGTGGGCTCGGCTAGCACACCCCTTTCCTGG 60.0 0.00
mm3_intergenic_Gm13810|B230118H07Rik_chr2_101038449_F Primer3: not found at this Tm N.d. 0.00
mm3_intergenic_Gm13810|B230118H07Rik_chr2_101038449_R Primer3: not found at this Tm N.d. 0.00
mm3_intergenic_4930509K18Rik|Gm23338_chr4_40412005_F Primer3: not found at this Tm N.d. 0.00
mm3_intergenic_4930509K18Rik|Gm23338_chr4_40412005_R Primer3: not found at this Tm N.d. 0.00

Off-target amplicon sequences with primers

These only list off-targets that have primers in the table above. Primers underlined, off-targets in bold.

mm3_intergenic_Gm3307|Otud7a_chr7_63614814 ACTCAAAGTTGGCCCCTAATGAAAAGAACATCAAGAGAAAATATGTAAATTATTCATGCTCGATTAGCAAAAATCCTGAA
ACATAGTCATGTCCTAT
ACTTGTTGATAACAGAGCCAAGAAGCTTACAACA
mm4_intergenic_Cd274|Pdcd1lg2_chr19_29400397 TGCAGACCCTTGTAAGTGGATCGAGTCCTTTCTGAATTCTAGATCAATAATGTTTAATTTGTTCCAAAAGTCGTCATCAT
TTCATGATATAGCCTGGAAAGACACGGTCATGTTTCTGGGTGGCCTGATCCAAAGCTTTC
mm4_intergenic_Tmc1|Zfand5_chr19_21074030 TCAGAGAAATGGAGGAGAAACCTAAAAATACGTTAGACATCTTTCTCTTTCTCTGTGTCTTTAGATACCCAGAAACATTG
CCATGTCTTTA
TCGTTGTTCCATATAGGATGGGAAGAATTTGTTTTCTGGGA
mm4_intron_Dnah8_chr17_30793736 TGTGAGCCATTAGAGATGTGAATCATTTAAAGATGTTATGGGTTCTACCTGTTTTTTTTAGAAAGTAAAATATCCTACCA
ATAGTAGTGTCTAAGGTCAATGTTATGTTTCTGGTAACAGTTGTCAGACGTGTTGGA
mm4_intergenic_Gm26235|Gm25392_chr18_18578527 AGCATTAAATGTGGCTTCATGCATGAAAAGATCAATGTTATGTTTCAGGCTTTGCTTACCTCTTAATTCTTCCCT
mm4_intergenic_Gm25715|N6amt1_chr16_86945902 TGGGAAGTTCCACGTTCATGAGAAATTGCCCCCAGAAGACATGGTCATGTATCTGGAGGACATCCTTATTTCTGCTGTCT
TTGTTTTCACGACACTAATAGGATTCATCTTATCAGACCCAGTCCCC
mm4_intron_Tmem229b_chr12_78989660 TCTCTTCCCAGGATCAGGTGATCTGTATTCTAAGGCCAAAAGACATGGTTAAACTTCTGGCATTATACCTACATGTCATT
AATGTATTCTGACACCTCATTTCAATGTGCCTGGTCACAATGTACCT
mm3_intergenic_Gm23342|Myt1l_chr12_29326735 CTGATCAAGAGCAGCACGTTAATAATTAATAATGACAAGGTTATGTTTCAGGTAGCTCAGTGTCAAGTTCATGTGATGAG
GATCTTCTGTTGGGAAAGGTCACCCCTTCCGTTCTTCTGGGGCCAGTGTATATCCTGC
mm4_intergenic_RP24-399L6.2|9230113P08Rik_chr9_35864710 AGTGTCAGGAGCCAACAAGTGGAGCTTGAAAAGTGGATAAGCAACAAGTCTCCCAAAGGAGGTGGCTATATTTCAGGGCT
GACTCATTAAGGAAGAGGTGGGATCTTGGA
mm4_intergenic_Gm11279|Gm11280_chr13_21904803 TGAAGGATCTCAGGGAGGACTCCCAGAAACATTTCCATTTCCTGTATTCCTTTATCTCATCTGTGCTCCTAATAAAGGAA
CCTAACATTGGTGCAGACAAGTAGGACTGCT
mm4_intron_Wdr48_chr9_119906695 GAGTGCTAACTCCCCACCTCGTGTATTTTTGTGAATCAGTTACTGAGGAAAGAGTTCTACATTGGCTTCCAAATCAAAGG
GCATGGACATGCTTCTGG
CTAAAAGCTTTGCGTTGGATTTTTGTTTGGGGTTTGGGTTGT
mm4_intron_Nrg2_chr18_36030766 ATCAGCTGGGGCCCAAATGCTCCAAAGGGAGTGGATATGTTTCTGGGCTCTGTTCTGTGGTCTGAGGACAGTTTGAGGAT
TCATTTTTCCACCCTAAGGTCCAAACTCTGAAAACGTGTTTCTGGATGTGCCA
mm4_intergenic_Ccdc85a|2810471M01Rik_chr11_28633770 CACCTCTGTTGACCTCTGACCTAGCTGTTTTAATCACACAGATACAGGAGATGGACAGGGTAATGTTTCAGGAGGCAATT
CTCCTTGGTAGTGCAGTGGG
mm4_intergenic_mmu-mir-6370|Gm22828_chr6_19605289 AACGTGGAATAGGAACATGGTAAACGGACAAGATAATGTTTCTGGGAAACTTTACACGTGAGGTGGATGCATGGTGA
mm3_intergenic_Gm26535|Gm24710_chr10_37883313 ATAGTCTCTTTTGAGCAGCTGAAAAATACATGATTATGTTTCAGGAAAATTTATTCCACTGTTTATGAATTCTTACAATA
AAGTCATAGTGTTATTGTTAGTTATTACTCACTTTTTTTGCATTCCCAGAAGAAACATCT
mm4_intron_H2-Q6_chr17_35428828 TATGGCCTGATTGCAGAGGGGCACTGTGGACATGGTTGTGTTTCAGGGACTCCCACAATCCCCTGTG
mm4_intron_H2-Q4_chr17_35383823 TATGGCCTGATTGCAGAGGGGCACTGTGGACATGGTTGTGTTTCAGGGACTCCCACAATCCCCTGTG
mm4_intergenic_Ednrb|RP24-312G4.2_chr14_104427783 GCGATAAGGCTTCAGCAAAGTACATGATGGATTTTAATGTGTGTGTTCCAGAAACAAAACCATGTCTCCTTTGCAGACCA
TAACTGTAGGACT
mm3_intergenic_Gm23225|Gm24127_chr14_92148520 GGTATAACCATTTCCTTCTCAATGCTTATTGTAAACTTAAATGCCAGAAACATATCCATGTATTTTGCTAACCCAATTTT
TTAAATATTTTTCATGCTCTGTGAATTGATTTCT
mm4_intergenic_4930480K15Rik|Nrxn1_chr17_90870150 CCCCATATAGCCTGCAGTTTCTTTGATGATGTGAATTCAGAGAGCATTTTCCACTTTGATTAGAAAGAAAGGGAAGTAAC
AGGACAAGGCTATGTTTTAGG
CAAACCAGAGCAGATTCAAGCCT
mm4_intergenic_Nrg3|Gm10713_chr14_39687638 AGGCTAGCATTGATGGACAGATAAATCACATAAGGGATGAGAGACATTCTACCTTGAAGATGTCTTTCTCTTGCAAATGC
CCAGAAACATTAAAATGTCTTTTTCACAGAGCTACACAGAGTGAGC
mm4_intron_Tbc1d8_chr1_39396731 CTTATGCTGCCGGCTTACCTCAACAGGGCATGGCTATGTGTCTGGAAAATGCAAGGATTAGCAACTCAGAAACAGGGCTC
CAAGGGCAGATGGTGCTTTTTCTCGGTGTTCCTGTCCACATG
mm4_intron_Ptprk_chr10_28354232 TCAGCTTCTTGGGTGATGGCTATCACATAGGGTATACTAGGAAGCTTGGTAGCACCAGAAACAAGACCATCTTCTTTGCC
CATTAAGGACTGTTGTTCA
mm4_intron_Atp8a1_chr5_67742950 TCTTCCCATGGCACTTTCCATAAGCAAAGAGTGTCCAAAAACATAACCATGTGCTAATTTACATGCTGCTTATATCTCCT
ATAACAAACAGGGCTGGGAAGGACACTTTCT
mm3_intron_Jak1_chr4_101189210 GGCGAAGAGGTTGTGACAGAGAGGAGAGATACCTGAGAAGAAAGGACATGGCTAAGTTACAGGCAGATGCACTTGGCCAT
CTGTTCCCA
mm4_intergenic_5S_rRNA|Cops8_chr1_90573178 GCCATCGTGACAGGTGACATCAGACTATTTTTCCAGGTCACAGGGCACTGACAAAGTGTGAGGCATAGATATAAGCCCGG
AAACACAGCCAAGTGCTTT
CCCAGGTAATCCAAGGGTGTGTGTGCT
mm4_intergenic_Gm20698/Vmn2r7/Gm26939|Vmn2r7/Gm26939_chr3_64780153 TGAAGGTTTCATAAACTTTAGGAGGTTTTATTTAGTAGGAAGTAGGTAACCAGGAACATATCCATTTTCTTTTCCTATCT
ACCATGATCTAAACTGCTAAAATCTGCTACATTCTGTCCTCTATGA
mm4_intergenic_2810055G20Rik|Gm11146_chr16_77564451 AGTCGCCACTTACATTACTAACATAGTTTTCCACTTTAGAATTTCATGAGCAAAAATAAGTACATGGTTGTATTTCTGGT
AGCAAGAACATATTAGAAAATAAATCTCGTTCACATTGAAGGTCT
mm4_intergenic_Gimap3|Tmem176b_chr6_48782100 GGAACACGCATCCCTGTTGAAAACATGAAACAAAATGTGTTTAAACTGGAAACATAACCATGTTTTCGGTAGAAACACTC
TGTAGGATAACAATAATCATTATTTCAAAACCAATGCATCACATACACTTTCA
mm4_intergenic_Gm24246|Pik3c3_chr18_29785027 GGTAGGTCTTGTCAGGGTGCACTAGTAATGTGTAAAGGACATTGTGAAATTTCAGGCTCTGGTGTGCCCAGCACATGGGA
AAATGGAATCACAAACCACCAGAAGGGTCAAGGAGGCCTGAACAA
mm4_intergenic_mmu-mir-6344|Irs1/Gm9747_chr1_82204713 AGCAGTGGTTGGTAAGTGCCAAGGGACAACTGAACTGCAATTCCTGAAACTTAACCTTGTCACTTTTGACAAGGCCCATT
CTAACCAAGTGATCCCTTATACTTAAGACATATTTGTCGGCAACTAGTGAGACA
mm4_intron_Tectb_chr19_55185828 TGGGTGTTTCCAAAGCAACAACTGCCCCCTTGCATATATTTCCATCAAATTACCAGAAACAGAACCATGCCCTACATAGA
ACAATGGATCCACCACACTGCTAATTGCTTAAGATTCTGGGAAGCCCAGAGTCTAAGC
mm4_intergenic_Lrrtm1|Ctnna2_chr6_77271533 AGCACTACCTTGGAATTCCTACTTGTATGTGAATGGACATGGAAATGTTCCTGGCTAGCAGGATGAAGTGGAAGAGAGAG
TGCAGAAAGGGGAGAAGATGAGCGTGA
mm4_intron_Ppp1r7_chr1_93353112 CCCAGTAAACAGGGCTAGCCCTTTATCCCCTAAACATTAACATGTCCTCTGGCAGGATTTGGTTTGTTGTTCAAGATCTC
TTTAACCTTGAAACCATGGGTCAGAATGGGA
mm4_intergenic_Atp9b|Sall3_chr18_80941962 GGAGGGGCAGCAATTACTCATGATAACTTTATTAGCTATAATTTGCGTTTATCTTCTGAAAGGACAGAGTGATGCTTCTG
G
GAGCTGTATATTAAATCCAAGGGCTTATACTTGACCCTGCCCTGATTCATTCCA
mm4_intron_Angpt4_chr2_151921024 TGTGCTGGCAAGTTTCTTCTTATTGAGACATAATTCACATATAGGAAAATCCATGTACTGCCATTTTATGACATAGGACA
ATGTCAACATCCCTAAACATTCCCATGTCCCTTTGAAGTTACACACACCCACCACAGG
mm4_intergenic_Gm25235|Atf6_chr1_170833044 TGGCCAAAACATCCACACCTTCTGTACCTCCTCCCTGTCTTCCTGGTGTGTTGCTTCTTCAGAGGACAGTGTTATGTTGC
AGG
ACAACTGGAATAACGAGACGCTGAATCCTGAGAGCCCCAC
mm4_intergenic_Qpct|Cdc42ep3_chr17_79139730 TCTGGCTCTCTGGTAGGCAAATACATGTTGAATGTGAATACAGGTATTTATCCAAGTATATGGTTGTGTTTCAGGACATG
TTCCAGGAGTAAAAAAATCACAGTGTATCAAGATGGGGTAGATGATGTAGTGGGG
mm4_intergenic_PCA3_1|Prune2_chr19_17070736 TGGAAACAGAGAGGGAAATTACATACTAGGAAGAAAGAGCATGGTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAGGTAGCCATTCTATCAGTGAA
mm4_intergenic_Gm8356|Gm3629_chr14_6544769 TGTCTTGACTTGTCTGTGACCAAATAGTCAGACTAAGACAAAGGTCAATAAAAGGGCATTGTTTTGTTTTTGGCTGTGTA
TAGGCTTTCTGTACTCTAGGCACTGATCTGGATCTCCG
mm4_intergenic_Gm6356|Gm10406_chr14_6981004 GTCTGAACTGTGTCAGGCCTTTTACTAGGGGTCTTTGTCTTGACTTGTCTGTGAACAAATAGTCAGACTAAGACAAAGGT
CAATAAAAGGGCATTGTTTTGTTTTTGGCCGTGTATAGGCTTTCTGTACCCTAGGCA
mm4_intergenic_Gm3542|Gm17126_chr14_5342386 TGTCTTGACTTGTCTGTGACCAAATAGTCAGACTAAGACAAAGGTCAATAAAAGGGCATTGTTTTGTTTTTGGCTGTGTA
TAGGCTTTCTGTACTCTAGGCACTGATCTGGATCTCCG
mm4_intergenic_Gm3373|Gm26557_chr14_5759555 TGTCTTGACTTGTCTGTGACCAAATAGTCAGACTAAGACAAAGGTCAATAAAAGGGCATTGTTTTGTTTTTGGCCGTGTA
TAGGCTTTCTGTACTCTAGGCACTGATCTGGATCTCCG
mm4_intergenic_Gm17223|Gm10413_chr14_3387992 CGGAGATCCAGATCAGTGCCTAGAGTACAGAAAGCCCATACACGGCCAAAAACAAAACAATGCCCTTTTATTGACCTTTG
TCTTAGTCTGACTATTTGTTCACAGACAAGTCAAGACAAAGACCCCT
mm4_intergenic_Gm17221|Gm17188_chr14_3181071 CGGAGATCCAGATCAGTGCCTAGAGTACAGAGAGCCCATACACGGCCAAAAACAAAACAATGCCCTTTTATTGACCTTTG
TCTTAGTCTGACTATTTGTTCACAGACAAGTCAAGACAAAGACCCCT
mm4_intergenic_Gm11107|Gm3115_chr14_4072370 CGGAGATCCAGATCAGTGCCTAGAGTACAGAAAGCCTATACACGGCCAAAAACAAAACAATGCCCTTTTATTGACCTTTG
TCTTAGTCTGACTATTTGGTCACAGACAAGTCAAGACAAAGACCCCTAGTAAAAGGCCTG
mm4_intergenic_Gm11105|Gm3138_chr14_4242069 CGGAGATCCAGATCAGTGCCTAGAGTACAGAAAGCCTATACACAGCCAAAAACAAAACAATGCCCTTTTATTGACCTTTG
TCTTAGTCTGACTATTTGGTCACAGACAAGTCAAGACA
mm4_intergenic_Gap|Gm2888_chr14_3023099 CGGAGATCCAGATCAGTGCCTAGAGTACAGAAAGCCCATACACGGCCAAAAACAAAACAATGCCCTTTTATTGACCTTTG
TCTTAGTCTGACTATTTGTTCACAGACAAGTCAAGACAAAGACCCCT
mm4_intergenic_Gm5105|1110002E22Rik/Gm5105_chr3_138061533 GGTTTGCCTTCTGTGAACGGTTTCTTCTCTTGAATAGCACACTTAGTTATCTTATTATGCCAGATTTGGAGACCCAAAAT
ATAAGCATGACCTTT
AATCAGCTGGTGGGTGGTCTGGCCTTCGTTCTTC
mm3_intergenic_Olfr576|Olfr578_chr7_102976242 AGGGCACACTACAACTCGAATATATTAGATACGATAATGTTTGTACTAAGGATAGATAAGCATATAGGATACTTATAAAG
GACAGGGTTATGTTAAAGG
AGAAATATAGGAGGCAGACTTAACTGGAGAG
mm4_intergenic_Gm5641|Gm26678_chr3_54579127 TGTTGTTTCTTCTGTGTTCCTACTTCATTAGAAATATGTTTCAGCATTCTGGATTTATCCAGAAATGTAACCATGTTATT
T
GGTTTTCAGGTTGGTAAGTGATATTTGTGA
mm4_intergenic_Gm25434|Rergl_chr6_139466643 CCAAAGACCACTTGCATGCAATAACTGATACTGAGGGTGTACTTTTCTCCTAACACATGATTACTACTGTATTTATTTTT
TGATAGTGAAAAGACTTGTTTAAGTTTCAGGCAACATTTGAACCCTTCCCAAGTCTTTGC
mm4_intron_Ncam1_chr9_49697711 GTCTGTGCTCTGACCCCTTCTATCTACCACACAGCAGAAACCAACTCCTATGAAGACATTATTTAGGTGCAGTTGCTTCC
AGATACATAACCATCACATTT
GGCTACTTCATAAGAATATCCCCA
mm4_intergenic_Megf9|Gm11227_chr4_70721682 GGGCACTTGTATTCTCCCACAGTTTGGAGGAAGAACTGAACAAGCATGGCAATAAAAGGCTCCTTCTCAGTCATAGGACA
TGGTATTGTTTTTGG
ACCTAAAGGCAACACATTTTCAGATGCT
mm4_intron_Spon1_chr7_113827975 AGGAGCTGAAAGATGCCACATTGTCCATTCTGAATGAACAGGAACCAAACAAATAAACAAACCCCAAAACAAAACCAAGT
CTTTT
GAGGAACTGCAGAAAAATAAGTCAACAAAGGGAGCCCG
mm4_intergenic_Gm12026|Gm12027_chr11_19454226 GGGACTTCATGAGTGAGGGCCAGAAACTCTACAGCACAGTTCTTCCTCTTCCCCAGAAACAGAAACATGTCCTACAGGTT
CAGTGAGAGGAGTAAAAGTGGTTAGTGAGCACCGG
mm4_intergenic_Gm22327|Zfand3_chr17_29985544 TTCGAATCCACGACCTCACCCCAGGGTCCCGGGAGACTTCTGTCCTCAGGCTTTGTCCCGTGAATATAAGCATGTCCTTT
GATTTCCTCTGTGATCTGGAATGTTCTGGCCAGGCTTTCTTCTG
mm3_exon_Gm6578_chr6_12099905 TTTGTCGTCTCCTGGCGTACTTTCTCATAGCAGTAGTCACAAAGGACATGTTTCTGCTTCAGGTAACCACATTCAGGGCA
AATGTCTATATTGTTCTTAATTTTAATAAGCTTTTGAGGGTTTCCTCTCCTACATCGG
mm3_exon_Cntn3_chr6_102164003 GCCATGTCTTCCACAGTCCTTTGAAAATCACATATTGAAAGACTCGACTCATCCATATCAAAGCCAGCAACATCACCATG
TTCTTT
TACAAATTTTACTTGGGAATGGAAAAGGCTGCCTG
mm3_intron_Col17a1_chr19_47684306 ATGTGTGTGGGAGGAGGGATGGAAGGAGATGGTTATGGTTCAGGCCACTGTATGCCACGTGAAGCCAGCGGTTGGCACCT
GCTCTGGCCACGCTGTCCATCTATCCCTGCCCTCCACATGA
mm4_intron_Ahdc1_chr4_133050869 GGGTGGGATGCCATGTCTAGATGGGGGACTCTGAGAAGGACATGGTTGTGTCCCTGGTCATACTTTGTGGGTGGAAGAGA
CTTCTCCCATGGCG
mm4_intron_A230071N21Rik_chr7_112527649 GTGGTCATGTCATGGAGCCAGATCTCAGAAATGGCTTAGGCCTACTGTCAAAACATAAAGGACAGGGTTCAGCTTCAGGT
CTAGTCTGTTCCTTACTGCTCTGT
mm4_intron_4930556M19Rik_chr15_10736219 CCCAACGAAGGATCTGGTTACATATTTACATGTGATACACAGAGGAAAAGAAAGCCTTTCTTCTTTTTAAAGGATTTGGT
TCTGCTTCTGG
TTTAAATCAATTTCCTTTCCCTGTTCAGATCCAAACACTTCGCATCCCT
mm3_intergenic_Robo1|Gm25699_chr16_73389541 TGCTTTCTGATGAGGTGAACTGAACAATCTGACATAATGCACAAAAGAACTTGGTTATGTTTAAAGAAAAATATTGACAC
ATTTAAAGTGTATCTAATTGTTTTGTTGTTAAATAGTAGAGCCAGGTAGTGTATTGT
mm4_intergenic_Gm8805|Crygf_chr1_65725868 TGTGCATTGAGGAAGTCTCTCTAAATGTCATGTTTGTGTTTCAGGCAGAAAATAATGGCTACAGGTTAGTGTTTACTT
mm4_intron_Diap3_chr14_86726916 TGTCAAGATTGGAGAAGTGAAGAAAAGGCCATGTTTTTGTTTATGGTGTTTAGGAGGGATTTTTCTCTCATAGCATCAAC
AGAGTATCCCATACCTAGATATTAGCTTTGAGAGGACTCTAGGAAAGGGG
mm4_intergenic_Poteg|Gm1698_chr8_27548647 GCTTGTCTTGAAGGGCATGCTTACTTTGTTTATATCTAGTTGCTTTAAGAAAGGAAATTGTGATGTTCCTGGAAAGTAGT
TAGGAAATCTCTGTGCAACTCCT
mm4_intergenic_Bbs9|Bmper_chr9_23145779 GGGGCATAGGAATGACACACTGTTACTTATAAAGGATTTGGTTATGTCACAGGCATATGTCACAGATTGAATGATGAGGG
CCATGTTCATTTTGGAGG
mm4_exon_Ap2a2_chr7_141631708 GCCACTGGCTCAATCTCAGAATCCAGAAACAGCGCCATGTGCTTTTAGGGTTAACCCAGGGCTGCGTGGCTCTGCTGGAT
GGTGACAGCGGTGGCTTAGAGGTATGTGACCTCATGGGCTAGGCAGTGTC
mm4_exon_Matn4_chr2_164389783 CTCAGAGATCCCTTCACCCGCCCTGCAACCCGCACCTCCAGCCCCAAAGGATATGGTTCTGCGTCAGGCTCTCGAGCGCC
CCCAGCGTGCGGCCCTGGAACTCCACGAGGCTTTC
mm4_intergenic_Prpf19|Zp1_chr19_10910417 AATAACAGCCACAAAGGGCAAATGGGCATGGTTATGGTTATGGTTGTATAGGAGAAGGTCTTTGCCATATTCAAAGGTAA
AAATGTGAGAGGGAAATGAAGCATGAAGGAACCCCG
mm4_intron_Gas7_chr11_67516235 AGCCTTCTGTGGAATCGCTCAGCCTGTGAGACCAGTCCAAAAAGCACATGGTTCTGATACTGGCCAGCATAGCTGCAGCC
ATGCCTGCTTGTCTATGTTGGACTCAGGTTCATGCAGC
mm4_intergenic_Gm5976|Tmem194b_chr1_52547245 AGGGCTTGGGAGAGTTCTACTAAAGGAAAAGGTTTTGTTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNAGTTGCTACACAGAAAGACCTCTTCAGCCATGGC
mm4_intergenic_Gm16477|Gpr26_chr7_131896789 CCCATAGTGTCATGACCATGGTATGTCAGAAACATAACCATGTTTGTGCTTTTCCACAATACCAGAATTTTTTTTTTCAG
AAATTTTTGTATAAAAATAAACTCACAAGTTTTGGTGATTTTGTTGGCAACA
mm3_intergenic_Nrcam|Stxbp6_chr12_44638821 TTGGCAGGGTTCAGCTCATTATTTTTTCATTTTCTCTCAAGCTATTAAGAGACTTTTGACTAGCCTGTGTTTAAATGTGT
GTTTACAAAGGACATTTTTATGTTTGTGGGCGAAGGATCTGATTCTGTGAC
mm4_intergenic_Ttc32|Gm7099_chr12_9192537 GCACCGTGGACTTTGGGTAATTTAGCCTTGGAAAGGAACCTGGAAGATAGGACATGGATATGATTGAGGATTTTGGAATT
TGCATCTGGGTATCTTCTGGGTGAGGTAGGAACTGGGACCTGGATGTAGGGA
mm4_intergenic_Gm23464|S100a2_chr3_90562004 GCCACTGCCTGGTATGTTCATGTGTTCCACTTCCAATGCAAGAAACTCCACAAACATTAGCATGTTCTTTCTGGACCATG
GCACCTGGTGGATGCAAGCACCTGTGAT
mm3_intergenic_Arid5b|1700040L02Rik_chr10_68401693 GTTTGCTTGCGTTGGTAGGGCTTTTTTGATAGGAGGTTGTGAGAGGAGATGGTTATGTTTATGATTAGCCAGTGTTTTCC
CCAGCCTCCCTTGCTGCATTCCTGA
mm4_intron_Nrxn3_chr12_90145376 TGGAAGATCCACCCGTCTATTAAGAAGACATTTGTGATTCATCCTAACACGTAAACATGTCCTTTGTTCTTCATTGTGGC
TTGGAAAAAGATCACACAGACCTGTCTGCTAATGACACTTGT
mm4_intergenic_Gm22227|Zfp703_chr8_26501939 AATAGACGCCGCTGACCTTCCCAGCAGCTCATAATATCCTGAAACCTCTCCGTGTCCTTTTGCAGCACCCTGGCCTGTGC
CCTGTTATGGGATTGGTTCTGTGTACAGTGCGGTGCAGAACAGAATCT
mm4_intron_Rp1l1_chr14_64019654 TGGTACTGGGGACATGGTGATACTAAGGGACCCAGTGGTGCTGGGGACATGATGCTATCAGGGACATGGTTATGTTGGAG
G
ATACAGAGATGCTGTGGGACATAGTGATGTCAGAGAACATGCCGACTGAAGGG
mm4_intron_Cdh13_chr8_118875049 ATCTTTCCCAGGCAGGCATCTATCCATCTCTAACTGATACCTGACTCTCCCAAGAGAATGTTAAAGGAAATGGTGATGGT
TTTGG
CTCTATACCATTTTCCTTCTTGAGACAGTTGTGTTTGGGATCTTTGGAGCAGGT
mm4_intergenic_Rpn1/mmu-mir-6376|Gata2_chr6_88106392 GTTCCAGTATTACCGCGCCTGAGATTCACGAAGTCCTGCTTCAGGAAGTCGAAGGCAAAGGACATGGTTTTGCTGGTGGC
CTAGCGGTGGCGCTCTGGCATCCCTGGGCTGGAACAGTGCATCATG
mm4_intron_Flt1_chr5_147631485 GGCAGCTTAGGGGAGTGAAACACCTTAAACCTCACCATGTCCTGTGCAGTCAGTGGCATTCCAGTTTCAGAGGTAAACAG
GGCCTCGGAGGGCAGGAAAATGAGGGCAGTGTTAGGCC
mm4_intergenic_Tmem37|Dbi_chr1_120102364 CCACACTCCACACACAGAGGTGTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTACTGCTAGAGTCACCCATTACC
mm4_intron_Mrgpra3_chr7_47592236 ATAGAGGCTGGCAGAGGACAGGCAACTTATTAATAACCCCAAAGACCAGGAACCCAACCATCTCCTTTGAGCAGTCCCTT
AGAATCATCACCTTGGTGTTTTGATTATATGTGGATGGGAGAAAAGCCA
mm4_intergenic_Mrgpra2b|Mrgpra3_chr7_47558442 ATAGAGGCTGGCAGAGGACAGGCAACTTATTAATAACCCCAAAGACCAGGAACCCAACCATCTCCTTTGAGCAGTCCCTT
AGAATCATCACCTTGGTGTTTTGATTATATGTGGATGGGAGAAAAGCCA
mm4_intron_Efna5_chr17_62659063 CAGGTTAAGAAGGGAAAGGAGACAAAATTTGGAATGTCAAGAAATTAGATGTTCACTGTCGTCATAAAAAGTATTGAATC
CATCCAAAGGAAATGGGTAGGTTTGGGGGCGGGGGGGAGGCTGGGTGTGCCTATCAGTC
mm4_intron_Ralyl_chr3_14109393 GCAATTGGCAGTTTAATCCAACACTTAACTTTAGTGCCACCAGAAAAAGAACCAAGTACTTTATAAATGCCGGTAAAGAA
TAAAGTTCAGTTAGAAAATAAGAAATGTCATGGATGTTTCCCGATGT
mm4_intron_Rab3ip_chr10_116939490 GGGGTCGTTAGCCATAGCAAAGGCTTCATCATAGGACAACCTAAGCTAAACACAAAAGAACATGGTTATTTCTAGGGACT
TACTTCAAACAAAATCAAAATACATAGGCTGTAAAACAATCCATATAAAGC
mm4_intron_Nsmce2_chr15_59517069 GACTTCCTGGGCCTGTCATGGCTTGGGCGCAGAGATGTTTTTTACAGCAGTGTTTCTGACAGATGGGAACATGCAGCGAC
CACAGCTCCCTGAAAGATCACCATGTCTCTTCTGCTTTCAGCACTTGCACCCTGA
mm4_intron_Col14a1_chr15_55465005 GCCTCGAGTTTTCATGCCACACAAATTTGGAACTGTAGTCATAATCTATATGATACTACCAGAAAACTAAGCATGTCCTA
T
TTTCTCCTAAAGTTGTATCTACATGTCTATGTACAGACACAAAAGGAGATTCATGAC
mm4_intergenic_Unc5d|4933433F19Rik_chr8_29752915 GCCGGGATAATGGAGTCTGGTAAGGTCCATTCTGGTGTTCCCAGAAAAATGACCAGGTGCTTTTAAAGGCCAATCCACAA
GTCTACCTACTTGCTGTCTTCATCCAACAGGG
mm4_intergenic_Slc19a3|Ccl20_chr1_83104736 AGACAGGTCTTTTGTTTGTTTGTTTGTTTTTGTTTTTTTTTTAAACCAAAGGACAGAGTTATCCTTCTGGGTTTTTGGAA
GATTCATCTGGCCTAAGCATGCACAA
mm4_intergenic_Serp2|Lacc1_chr14_76615262 ACAACTGCCAAACCATTGCCCCAGCACGTGATATGCAGGCTGCTTTCGATTTAGGTAAAAGGGCATGGTTGTTTTTTAGG
ATTCCTGATCTACGCATTACACTGTTTGCACAGGTAGATGGGCGGATC
mm4_intergenic_Pacrg|A230009B12Rik_chr17_10439142 GGCCCTGGTCACACATAACAGGGATACTGTGACTGTGGAGCAACCTGATGAAAGGACTACTCTCCCAGAACGATAACCAT
GGTCTTT
GGATGTTGAAATCACGTGGGCTTCAGAGAGACCCTT
mm4_intergenic_Gm833|Gm25779_chr4_152922320 CTGTCAAGGAGCTGAGCACATGCTCCAGGCAAAGGTCTACAGCATATGTCCAAACTGCAGGAGAGGCAAAGACCTTGGTT
ATCTTTCTGG
GAGGCCACTAAGGTTTATTTCAGAGA
mm4_intergenic_Gm24382|Mbnl1_chr3_60203133 ACAGAGAGAGTGTGCTGAACAAGCATGTAACTCAAGGGCATGGTTATTTTTAAGGGTTTATTTTATGACTTTAGGCAGAG
GAAAGAACCAATCAGC
mm4_intergenic_Gm13988|Sema6d_chr2_123803202 CACCTGTGAATGGTGCAAGTTAACATGGCTTTTTGTAGTGCCATCTCAAGAAACACTATCATCGACTAGTCCAGTGGCCT
TAAAAATATCCATGACCTTT
GCCCCCTTGTCACTTACACAAGGTAATAATGGCT
mm4_intergenic_Fbxo15|Gm25453_chr18_85297064 GCCTCCCATGCCAAAATACCTCAAAGTGTAAGTCGAATTTTACAACGGCAAAGGGACAAGGTTATTTCTCTGGTAGACTG
GGGCATCAGTACTTGCCAGTGAGTCTCAAACAACG
mm4_intergenic_Camsap2|9230116N13Rik_chr1_136383615 ACACAGAGATCCCAGGACTTTTCTTTCTAGGCAACAGATTTCCAGAAAGATTACCATGTTCATTGAAGCCATGTGTGTGG
CTTTAAGTAGACAGT
mm4_intergenic_6330403L08Rik/Pdgfa|Prkar1b_chr5_139011242 CCACTGGAGCCATCAACTGTATTTCTTCATGAGAGGACACTGTTATCTTTCTGGATTGGAAGCAAAATTTCACTGGTTCC
CAGTGCCTCCTTGGCCTGTCAGTTT
mm4_intergenic_1700019M22Rik|Gm26480_chr12_96169874 ACCAGGAGATCCCAAAAGTAGAATAGAAGACATGGTTATCTATCAGGTTGTCATGTTGTTTCTTGATTTAGCTTTATATT
CAAACTCAGCTCATGTCTCCA
mm4_exon_Spc25_chr2_69199492 GCCTGCACGTGATCTTAGGAAGCCCCAAAACACTATCCATTCTAGCCAATTAACGTATACAGAGATAACAACAGCCTGAA
AAACACCCATGTGCTTT
TTAACCTAGCAATCCCCCTCTGAGAAAGGCTCCTCCCA
mm4_exon_Ntrk2_chr13_58934623 TCCCTGGCTTTCTTTGGTCCCCCAGAAGAAATCTGCTCTCTGAGGATTCACAATGGTGTGTATTTTATTTTTACAGTGCC
AGGAAGAGAGAAGGGACATTTTTATTTTTCTGGAGTTAGGTCTGTGCTCTCTGACAGG
mm3_intergenic_Pdk3|Supt20-ps_chrX_93912605 GTTCTCTTCCCATGGCCCAATTCTAGCCCTACTACTCTTCCATCAAAGGCCATGGTCATCTTTCAGGAACCTTTCCCATG
GTAAGCACTTCCAGGAAAGGGGTGTGCTAG

Input file for Crispresso

Crispresso, written by Luca Pinello, is a software package to quantify the Cas9-induced mutations on off- or on-targets.

Click here to download an amplicon input file for Crispresso. For each off-target, it includes the off-target name, its PCR amplicon and the guide sequence. Keep a copy of this file.

After sequencing, run CRISPRessoPooled. The tool will map the reads to the amplicons and analyse the mutations:
CRISPRessoPooled -r1 Reads1.fastq.gz -r2 Reads2.fastq.gz -f crisporAmplicons_mN1FkRligAThyqKv3eg3.txt --name MY_EXPERIMENT

Version 5.01 - Documentation  - Contact us - Downloads/local installation - Citation - License
CRISPR/Cas9 Guide Designer for chordate vertebrate ecdysozoans lophotrochozoans protostomes spongi corals plants butterflies metazoans genomes fruitflies insects nematodes mammals.