CRISPOR

DNA break repair outcome predictions

Guide: GCCTGAACCGCCAGAGAAGA TGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

mh- Score	Sequence	Effect
	`GAAGCCGTGAGCAGCCCGCTGCCTGAACCGCCAGAGAAGATGGTGCCCCACGGTTGCCTGAGCCCGCGAGCCGGCCCTCC`	(Wild-type sequence)
283	`GCAGCCCGCTGCCTGAACCGCC---------------CCACGGTTGCCTGAGCCCGCGAG`	`15 bp deleted → no frameshift`
213	`GCAGCCCGCTGCCTGAACCGCCA-----------------CGGTTGCCTGAGCCCGCGAG`	`17 bp deleted → frameshift`
210	`GCAGCCCGCTGCC------------------------CCACGGTTGCCTGAGCCCGCGAG`	`24 bp deleted → no frameshift`
191	`GCAGCCCGCTGCCTGA-----------------------------------GCCCGCGAG`	`35 bp deleted → frameshift`
179	`GCAGCCCGCTGCCTGAACCGCC----------------CACGGTTGCCTGAGCCCGCGAG`	`16 bp deleted → frameshift`
169	`GCAGCCC-------------------------------CACGGTTGCCTGAGCCCGCGAG`	`31 bp deleted → frameshift`
163	`GCAGCCCGCTGCCTGAACCGCC--------------------------TGAGCCCGCGAG`	`26 bp deleted → frameshift`
162	`GCAGCCCGCTGCCTGAACC------------------CCACGGTTGCCTGAGCCCGCGAG`	`18 bp deleted → no frameshift`
154	`GCAGCCCGCTGCCTGAACC-------------------CACGGTTGCCTGAGCCCGCGAG`	`19 bp deleted → frameshift`
147	`GCAGCCCGCTGCCTGAACC--------------------ACGGTTGCCTGAGCCCGCGAG`	`20 bp deleted → frameshift`
136	`GCAGCCCGCTGCCTGAACCGCCAGAG--------------------------CCCGCGAG`	`26 bp deleted → frameshift`
133	`GCAGCCCGCTGCCTGAACCG----------------------GTTGCCTGAGCCCGCGAG`	`22 bp deleted → frameshift`
132	`GCAGCCCGCTGCCTGAACCGC------------------------------------GAG`	`36 bp deleted → no frameshift`
128	`GCAGCCCGCTGCCTG-----------------GTGCCCCACGGTTGCCTGAGCCCGCGAG`	`17 bp deleted → frameshift`
121	`GCAGCCCGCTGCCTGAACCGCC--------------------------------CGCGAG`	`32 bp deleted → frameshift`
121	`GCAGCCC--------------------------------ACGGTTGCCTGAGCCCGCGAG`	`32 bp deleted → frameshift`
118	`GCAGCCCGC------------------------------------------------GAG`	`48 bp deleted → no frameshift`
114	`GCAGCCCGCTGCC-------------------------CACGGTTGCCTGAGCCCGCGAG`	`25 bp deleted → frameshift`
110	`GCAGCCCGCTGCCTG--------------------CCCCACGGTTGCCTGAGCCCGCGAG`	`20 bp deleted → frameshift`
109	`GCAGCCCGCTGCC--------------------------ACGGTTGCCTGAGCCCGCGAG`	`26 bp deleted → frameshift`
105	`GCAGCCCGCTGCCTGAACCGCCAGAGAAGA---------------------GCCCGCGAG`	`21 bp deleted → no frameshift`
105	`GCAGCCCGCTG---------------------GTGCCCCACGGTTGCCTGAGCCCGCGAG`	`21 bp deleted → no frameshift`
103	`GCAGCCCGC---------------------------CCCACGGTTGCCTGAGCCCGCGAG`	`27 bp deleted → no frameshift`
95	`GCAGCCCGCTGCCTGAACCGCCAGAGAAG-----------------------CCCGCGAG`	`23 bp deleted → frameshift`
95	`GCAGCCCGCTGCCTGAAC-----------------------GGTTGCCTGAGCCCGCGAG`	`23 bp deleted → frameshift`
94	`GCAGCCCGCTGCCTGAACC-----------------------------TGAGCCCGCGAG`	`29 bp deleted → frameshift`
91	`GCAGCCCGCTGCCTGAACCGCCAGAG----------------------------------`	`34 bp deleted → frameshift`
90	`GCAGCCCGCTGCCTGAACCGCCAGAGA------------------------GCCCGCGAG`	`24 bp deleted → no frameshift`
89	`GCAGCCC------------------------------CCACGGTTGCCTGAGCCCGCGAG`	`30 bp deleted → no frameshift`
77	`GCAGCCCGCTGCC-----------------------------------------CGCGAG`	`41 bp deleted → frameshift`
76	`GCAGCCCGCTGCCTGAACCGCC---------------------------------GCGAG`	`33 bp deleted → no frameshift`
76	`GCAGCC---------------------------------ACGGTTGCCTGAGCCCGCGAG`	`33 bp deleted → no frameshift`
74	`GCAGCCCGCTGCCTGAACCGCCAG----------------------------CCCGCGAG`	`28 bp deleted → frameshift`
73	`GCAGCCCG----------------------------------GTTGCCTGAGCCCGCGAG`	`34 bp deleted → frameshift`
73	`GC----------------------------------CCCACGGTTGCCTGAGCCCGCGAG`	`34 bp deleted → frameshift`
73	`GCAGCC------------------------------------------TGAGCCCGCGAG`	`42 bp deleted → no frameshift`
71	`GCAGCCCGCTG---------------------------------------AGCCCGCGAG`	`39 bp deleted → no frameshift`
70	`GCAGCCCGCTGCCTGAACCGCCAGAGAAGA-----------------------------G`	`29 bp deleted → frameshift`
69	`GCAGCCCGCTGCCTGAACC-----------------------------------CGCGAG`	`35 bp deleted → frameshift`
63	`GCAGCCCGCTGCCTGAACCGCCAGAGAAG-------------------------------`	`31 bp deleted → frameshift`
63	`GCAGCCCGCTGCCTG-------------------------------CCTGAGCCCGCGAG`	`31 bp deleted → frameshift`
60	`GCAGCCCGCTGCCTGAACCGCCAGAGA--------------------------------G`	`32 bp deleted → frameshift`
60	`GCAGCCCGCTGCCTGAACCG--------------------------------------AG`	`38 bp deleted → frameshift`
60	`GCAGCCCGC--------------------------------------CTGAGCCCGCGAG`	`38 bp deleted → frameshift`
51	`GCAGCCC-----------------------------------------TGAGCCCGCGAG`	`41 bp deleted → frameshift`
49	`GCAGCCCGCTGCCTGAACCGCCAG------------------------------------`	`36 bp deleted → no frameshift`
48	`GCAGCCCGCTGCC------------------------------------------GCGAG`	`42 bp deleted → no frameshift`
47	`GCA-------------------------------------CGGTTGCCTGAGCCCGCGAG`	`37 bp deleted → frameshift`
44	`GCAGCCCGC--------------------------------------------CCGCGAG`	`44 bp deleted → frameshift`
42	`GCAGCCCGCTGC---------------------------------------------GAG`	`45 bp deleted → no frameshift`
42	`GC---------------------------------------------CTGAGCCCGCGAG`	`45 bp deleted → no frameshift`
38	`GCAGCCC-----------------------------------------------CGCGAG`	`47 bp deleted → frameshift`
34	`GCAGCCCGCTGCCTGA-------------------------------------------G`	`43 bp deleted → frameshift`
34	`GCAGCC-------------------------------------------------GCGAG`	`49 bp deleted → frameshift`
32	`GCAGCCCG--------------------------------------------------AG`	`50 bp deleted → frameshift`
31	`GC---------------------------------------------------CCGCGAG`	`51 bp deleted → no frameshift`
29	`GCAGC----------------------------------------------------GAG`	`52 bp deleted → frameshift`
25	`GC-------------------------------------------------------GAG`	`55 bp deleted → frameshift`
18	`GCAG--------------------------------------------------------`	`56 bp deleted → frameshift`

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