CRISPOR

DNA break repair outcome predictions

Guide: CCTGGGATTAGGAGCTTACT TGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

mh- Score	Sequence	Effect
	`TCAGCCAAGATCCCAGGGAGCCTGGGATTAGGAGCTTACTTGGGGGTTTTCCCCCTCCCCCCCTCTGGAGAGTCCGGGGA`	(Wild-type sequence)
284	`TCCCAGGGAGCCTGGG------------------GGTTTTCCCCCTCCCCCCCTCTGGAG`	`18 bp deleted → no frameshift`
232	`TCCCAGGGAGCCTGGG-------------------GTTTTCCCCCTCCCCCCCTCTGGAG`	`19 bp deleted → frameshift`
230	`TCCCAGGGAGCCTGGGATTAGG-----------GGGTTTTCCCCCTCCCCCCCTCTGGAG`	`11 bp deleted → frameshift`
220	`TCCCAGGGAGCCTGGG--------------------TTTTCCCCCTCCCCCCCTCTGGAG`	`20 bp deleted → frameshift`
219	`TCCCAGGGAGCCTGGGATTAGG------------GGTTTTCCCCCTCCCCCCCTCTGGAG`	`12 bp deleted → no frameshift`
208	`TCCCAGGGAGCCTGGGATTAGG-------------GTTTTCCCCCTCCCCCCCTCTGGAG`	`13 bp deleted → frameshift`
198	`TCCCAGGGAGCCTGGGATTAGG--------------TTTTCCCCCTCCCCCCCTCTGGAG`	`14 bp deleted → frameshift`
170	`TCCCAGGGAGCCTGGG-----------------GGGTTTTCCCCCTCCCCCCCTCTGGAG`	`17 bp deleted → frameshift`
163	`TCCCAGGG--------------------------GGTTTTCCCCCTCCCCCCCTCTGGAG`	`26 bp deleted → frameshift`
155	`TCCCAGGG---------------------------GTTTTCCCCCTCCCCCCCTCTGGAG`	`27 bp deleted → no frameshift`
148	`TCCCAGGG----------------------------TTTTCCCCCTCCCCCCCTCTGGAG`	`28 bp deleted → frameshift`
140	`TCCCAGGGAGCCTGG---------------------TTTTCCCCCTCCCCCCCTCTGGAG`	`21 bp deleted → no frameshift`
134	`TCCCAGGGAGCCTGGGATTAGGAGCTTACT----------------CCCCCCCTCTGGAG`	`16 bp deleted → frameshift`
115	`TCCCAGGGAGCCTGGGATTAGGAG------------------------------------`	`36 bp deleted → no frameshift`
115	`TCCCAGGGAGCCTGGGATTAGGAGCTT-----------TTCCCCCTCCCCCCCTCTGGAG`	`11 bp deleted → frameshift`
114	`TCCCAGGG-------------------------GGGTTTTCCCCCTCCCCCCCTCTGGAG`	`25 bp deleted → frameshift`
110	`TCCCAGGGAGCCTGGGATTAGGAGCT--------------------CCCCCCCTCTGGAG`	`20 bp deleted → frameshift`
109	`TCCCAGGGAGCCTGGGATTAGGAGCTT------------TCCCCCTCCCCCCCTCTGGAG`	`12 bp deleted → no frameshift`
104	`TCCCAGGGAGCCTGGGATTAGGAGCTT-------------CCCCCTCCCCCCCTCTGGAG`	`13 bp deleted → frameshift`
99	`TCCC---------------------------------------CCTCCCCCCCTCTGGAG`	`39 bp deleted → no frameshift`
96	`TCCCAGGGAGCCT---------------------------------CCCCCCCTCTGGAG`	`33 bp deleted → no frameshift`
94	`TCCCAGG-----------------------------TTTTCCCCCTCCCCCCCTCTGGAG`	`29 bp deleted → frameshift`
90	`TCCCAGGGAGCCTGGGATTAGGAGCTTACT------------------------CTGGAG`	`24 bp deleted → no frameshift`
89	`TCCCAGGGAGCC------------------------------CCCTCCCCCCCTCTGGAG`	`30 bp deleted → no frameshift`
84	`TCCCAGGGAGCC-------------------------------CCTCCCCCCCTCTGGAG`	`31 bp deleted → frameshift`
81	`TCCCAGGGAGCCTGGGATTAGGAGCTTACT--------------------------GGAG`	`26 bp deleted → frameshift`
81	`TCCCAGGGAGCCTGG-------------------------------------------AG`	`43 bp deleted → frameshift`
81	`TCCC----------------------------------------CTCCCCCCCTCTGGAG`	`40 bp deleted → frameshift`
80	`TCCCAGGGAGCC--------------------------------CTCCCCCCCTCTGGAG`	`32 bp deleted → frameshift`
77	`TCCCAGGGAGCCTGGGATT-------------------TTCCCCCTCCCCCCCTCTGGAG`	`19 bp deleted → frameshift`
77	`TCCC-----------------------------------------TCCCCCCCTCTGGAG`	`41 bp deleted → frameshift`
74	`TCCCAGGGAGCCTGGGATTAGGAGCT----------------------------CTGGAG`	`28 bp deleted → frameshift`
73	`TCCCAGGGAGCCTGGGATT--------------------TCCCCCTCCCCCCCTCTGGAG`	`20 bp deleted → frameshift`
73	`TCCC---------------------------------------------CCCCTCTGGAG`	`45 bp deleted → no frameshift`
70	`TCCCAGGGAGCCTGGGATT---------------------CCCCCTCCCCCCCTCTGGAG`	`21 bp deleted → no frameshift`
66	`TCCCAGGGAGCCTGGGATTAGGAGCT------------------------------GGAG`	`30 bp deleted → no frameshift`
66	`TCCCAGGGAGCC------------------------------------CCCCCTCTGGAG`	`36 bp deleted → no frameshift`
64	`TCCCAGGGAGCCT-----------------------------------------CTGGAG`	`41 bp deleted → frameshift`
62	`TCCCAGGGAGCC-------------------------------------CCCCTCTGGAG`	`37 bp deleted → frameshift`
61	`TCCCAGGGAGCCTGGGA------------------------------------------G`	`42 bp deleted → no frameshift`
60	`TCCCAGGGAGCC--------------------------------------CCCTCTGGAG`	`38 bp deleted → frameshift`
60	`TCCC--------------------------------------CCCTCCCCCCCTCTGGAG`	`38 bp deleted → frameshift`
60	`TCCC----------------------------------------------CCCTCTGGAG`	`46 bp deleted → frameshift`
57	`TCCCAGGGAG--------------------------------------------------`	`50 bp deleted → frameshift`
57	`TCCC-----------------------------------------------CCTCTGGAG`	`47 bp deleted → frameshift`
56	`TCCCAGGGAGCC---------------------------------------CCTCTGGAG`	`39 bp deleted → no frameshift`
54	`TCCC------------------------------------------------CTCTGGAG`	`48 bp deleted → no frameshift`
54	`TCCCAGGGAGCC----------------------------------------CTCTGGAG`	`40 bp deleted → frameshift`
51	`TCCC-------------------------------------------------TCTGGAG`	`49 bp deleted → frameshift`
48	`TCC------------------------------------------TCCCCCCCTCTGGAG`	`42 bp deleted → no frameshift`
44	`TCCC--------------------------------------------CCCCCTCTGGAG`	`44 bp deleted → frameshift`
42	`TCCCAGGGAGCCTGGGATTAG---------------------------------------`	`39 bp deleted → no frameshift`
32	`TCC--------------------------------------------------TCTGGAG`	`50 bp deleted → frameshift`
31	`TCCCAGG---------------------------------------------------AG`	`51 bp deleted → no frameshift`
21	`TC-----------------------------------------------------TGGAG`	`53 bp deleted → frameshift`
20	`TCCCAG------------------------------------------------------`	`54 bp deleted → no frameshift`

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