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Contents:

PCR primers for off-targets of TCCTTTCGGCTCTCCATCCC CGG

In the table below, Illumina Nextera Handle sequences have been added and highlighted in bold. Primers for the on-target have been added for convenience. The table below is sorted by the CFD off-target score. Sites with very low CFD scores < 0.02 are unlikely to be cleaved, see our study Haeussler et al. 2016, Figure 2.

In the protocol by Matthew Canver, Harvard, two PCRs are run: one PCR to amplify the potential off-target, then a second PCR to extend the handles with Illumina barcodes. Please click here to download the protocol. Alternatively, you can have a look at Fu et al, 2014.

If a primer was not found, the reason is usually that the region around the off-target is too repetitive. To avoid unspecific primers, all repeats are masked for the primer design (not for off-target search). If you think that we should change the parameters here or should use different primer3 settings, please let us know.

Maximum amplicon length:     Primer Tm:

NamePrimer Sequence Tm CFD Score
ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52313838_F Primer3: not found at this Tm N.d. 1.00
ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52313838_R Primer3: not found at this Tm N.d. 1.00
mm3_intron_Erbb2_chr11_98415644_F TCGTCGGCAGCGTCCCTTTCTCTTGGGCCCTCTG 60.0 0.60
mm3_intron_Erbb2_chr11_98415644_R GTCTCGTGGGCTCGGCCTGCCCACATCATTCCCAT 60.1 0.60
mm3_intron_Llgl2_chr11_115854045_F TCGTCGGCAGCGTCGCCTGTGGCCTCTGTGAG 60.0 0.59
mm3_intron_Llgl2_chr11_115854045_R GTCTCGTGGGCTCGGCCGACAGAGCTCCACATCTG 60.1 0.59
mm4_intron_Gm20681_chr8_122374323_F TCGTCGGCAGCGTCGCAAGAGTGAGAAGAGGGGAC 60.0 0.42
mm4_intron_Gm20681_chr8_122374323_R GTCTCGTGGGCTCGGAGAGGCTGGGGAGATGAACT 59.9 0.42
mm4_intron_Slamf8_chr1_172589020_F TCGTCGGCAGCGTCCCAATGCAAGATAAAGGTGGAAACT 60.0 0.40
mm4_intron_Slamf8_chr1_172589020_R GTCTCGTGGGCTCGGTCCCGCTTCACTCTCAAACC 59.9 0.40
mm4_intergenic_Gm25660|Slc27a6_chr18_58498801_F Primer3: not found at this Tm N.d. 0.40
mm4_intergenic_Gm25660|Slc27a6_chr18_58498801_R Primer3: not found at this Tm N.d. 0.40
mm4_intergenic_Gm22184|Asprv1_chr6_86606636_F TCGTCGGCAGCGTCAAGGTTGTCAGGGCCCAAAT 59.8 0.39
mm4_intergenic_Gm22184|Asprv1_chr6_86606636_R GTCTCGTGGGCTCGGGCCATCAGACTCCTGGTTCC 60.1 0.39
mm3_intron_Ece1_chr4_137922950_F TCGTCGGCAGCGTCTCAGAGCATCCTGTTGCCAG 60.0 0.38
mm3_intron_Ece1_chr4_137922950_R GTCTCGTGGGCTCGGGGGCACTCTTTGAGTCCTGG 60.3 0.38
mm4_intergenic_Gm15668|Kif22-ps_chr1_67389534_F TCGTCGGCAGCGTCGGTTTTCAGCATGCACTTCCA 59.6 0.37
mm4_intergenic_Gm15668|Kif22-ps_chr1_67389534_R GTCTCGTGGGCTCGGGCCTGGACATATAAAAGACATTCCC 59.7 0.37
mm4_intergenic_Gm6462|Gm25586_chr1_28959563_F TCGTCGGCAGCGTCCCCTACCATGGGCAGAACC 59.7 0.37
mm4_intergenic_Gm6462|Gm25586_chr1_28959563_R GTCTCGTGGGCTCGGACCTCTTACTGAAATGACTCTCAA 57.3 0.37
mm4_intron_A530013C23Rik_chr2_167709856_F Primer3: not found at this Tm N.d. 0.36
mm4_intron_A530013C23Rik_chr2_167709856_R Primer3: not found at this Tm N.d. 0.36
mm4_intergenic_Pde2a|Art2a-ps_chr7_101543249_F Primer3: not found at this Tm N.d. 0.36
mm4_intergenic_Pde2a|Art2a-ps_chr7_101543249_R Primer3: not found at this Tm N.d. 0.36
mm4_intron_Pxdc1_chr13_34644702_F Primer3: not found at this Tm N.d. 0.35
mm4_intron_Pxdc1_chr13_34644702_R Primer3: not found at this Tm N.d. 0.35
mm4_intron_Kcnq1_chr7_143148909_F TCGTCGGCAGCGTCGGCCACCTTGGATGATGACA 60.0 0.35
mm4_intron_Kcnq1_chr7_143148909_R GTCTCGTGGGCTCGGGCTCTGATTCCAAGGGCACT 60.0 0.35
mm4_intergenic_Gm649|Gm14805_chrX_89337902_F TCGTCGGCAGCGTCGGGGATAAATCACCCTCCATGG 60.2 0.34
mm4_intergenic_Gm649|Gm14805_chrX_89337902_R GTCTCGTGGGCTCGGAGGTCTCACAAATCTGTCCCA 58.6 0.34
mm4_intron_Tnni1_chr1_135803713_F TCGTCGGCAGCGTCCCTCAGAGAACTCAGCCTGC 60.1 0.34
mm4_intron_Tnni1_chr1_135803713_R GTCTCGTGGGCTCGGTCCGACTATCCAGGCCAAGA 60.0 0.34
mm3_intergenic_Gulp1|Col3a1_chr1_45225820_F Primer3: not found at this Tm N.d. 0.32
mm3_intergenic_Gulp1|Col3a1_chr1_45225820_R Primer3: not found at this Tm N.d. 0.32
mm4_intron_Vmn2r19_chr6_123313854_F TCGTCGGCAGCGTCGGAGCAGCTGTCTTCCTGAG 60.1 0.32
mm4_intron_Vmn2r19_chr6_123313854_R GTCTCGTGGGCTCGGGCCAGAGGCTGTGATAGAAAGA 59.8 0.32
mm4_intergenic_Gm11468|Gm14268_chr2_166333558_F Primer3: not found at this Tm N.d. 0.31
mm4_intergenic_Gm11468|Gm14268_chr2_166333558_R Primer3: not found at this Tm N.d. 0.31
mm3_intron_Col24a1_chr3_145448799_F Primer3: not found at this Tm N.d. 0.29
mm3_intron_Col24a1_chr3_145448799_R Primer3: not found at this Tm N.d. 0.29
mm3_intron_Cad_chr5_31062089_F Primer3: not found at this Tm N.d. 0.28
mm3_intron_Cad_chr5_31062089_R Primer3: not found at this Tm N.d. 0.28
mm4_intergenic_Atl3|Pla2g16_chr19_7549687_F TCGTCGGCAGCGTCGCCCAGAAAGACTTGTTGGC 59.6 0.27
mm4_intergenic_Atl3|Pla2g16_chr19_7549687_R GTCTCGTGGGCTCGGCCCTCCCCTGCTAAACTGTG 60.0 0.27
mm3_intergenic_Rhot1|Rhbdl3_chr11_80295030_F Primer3: not found at this Tm N.d. 0.27
mm3_intergenic_Rhot1|Rhbdl3_chr11_80295030_R Primer3: not found at this Tm N.d. 0.27
mm4_intergenic_Gpr89|Gja8_chr3_96911748_F Primer3: not found at this Tm N.d. 0.27
mm4_intergenic_Gpr89|Gja8_chr3_96911748_R Primer3: not found at this Tm N.d. 0.27
mm4_intergenic_Pcdh8|Gm6999_chr14_79828059_F Primer3: not found at this Tm N.d. 0.26
mm4_intergenic_Pcdh8|Gm6999_chr14_79828059_R Primer3: not found at this Tm N.d. 0.26
mm4_intergenic_Gm25754|Gm24555_chr1_83826538_F Primer3: not found at this Tm N.d. 0.26
mm4_intergenic_Gm25754|Gm24555_chr1_83826538_R Primer3: not found at this Tm N.d. 0.26
mm4_intergenic_Frk|Gm26341_chr10_35050311_F Primer3: not found at this Tm N.d. 0.26
mm4_intergenic_Frk|Gm26341_chr10_35050311_R Primer3: not found at this Tm N.d. 0.26
mm4_intergenic_Ackr3|5S_rRNA_chr1_90234333_F Primer3: not found at this Tm N.d. 0.26
mm4_intergenic_Ackr3|5S_rRNA_chr1_90234333_R Primer3: not found at this Tm N.d. 0.26
mm4_intron_Ppfibp1_chr6_147017854_F Primer3: not found at this Tm N.d. 0.26
mm4_intron_Ppfibp1_chr6_147017854_R Primer3: not found at this Tm N.d. 0.26
mm4_intergenic_5033426O07Rik|Gm26815_chr8_121249014_F TCGTCGGCAGCGTCCCCTCATGTCACACTTCCCC 60.0 0.25
mm4_intergenic_5033426O07Rik|Gm26815_chr8_121249014_R GTCTCGTGGGCTCGGGGACCCATGATGCAATGGGA 60.1 0.25
mm4_intergenic_Ccdc154|BC003965_chr17_25173861_F TCGTCGGCAGCGTCGCAAGTTAGGGCTGGCAGTA 60.0 0.25
mm4_intergenic_Ccdc154|BC003965_chr17_25173861_R GTCTCGTGGGCTCGGCCCTCTAAGCCAGGTCCTCT 60.0 0.25
mm3_intergenic_Gm26963|Ypel5_chr17_72737228_F Primer3: not found at this Tm N.d. 0.24
mm3_intergenic_Gm26963|Ypel5_chr17_72737228_R Primer3: not found at this Tm N.d. 0.24
mm4_intergenic_Vimp|Chsy1/Gm26827_chr7_66092633_F TCGTCGGCAGCGTCTTCCTTGGCATCGTCTTCCC 60.0 0.24
mm4_intergenic_Vimp|Chsy1/Gm26827_chr7_66092633_R GTCTCGTGGGCTCGGGCACATCTTCCAAATCAGGCC 59.8 0.24
mm4_intron_Tns1_chr1_73926758_F TCGTCGGCAGCGTCAGCCCGGTACAGAGACAGAA 60.2 0.24
mm4_intron_Tns1_chr1_73926758_R GTCTCGTGGGCTCGGCTACACGTTCAGTCCGCCTT 60.0 0.24
mm4_intergenic_Sorbs1|Aldh18a1_chr19_40521365_F Primer3: not found at this Tm N.d. 0.24
mm4_intergenic_Sorbs1|Aldh18a1_chr19_40521365_R Primer3: not found at this Tm N.d. 0.24
mm4_intergenic_Gm13508|Gm25215_chr2_49348546_F TCGTCGGCAGCGTCCCTGCGGAAGAAGATGGTGA 59.7 0.23
mm4_intergenic_Gm13508|Gm25215_chr2_49348546_R GTCTCGTGGGCTCGGGAATGCCCAGGCCCTATAGC 60.3 0.23
mm4_intron_Cdkn1a_chr17_29095488_F TCGTCGGCAGCGTCATCGTGACGTGTTTTGTGGC 59.6 0.23
mm4_intron_Cdkn1a_chr17_29095488_R GTCTCGTGGGCTCGGTGGTGAGTCAGTTTCCTGCC 59.8 0.23
mm4_intergenic_Gm12708|Gm830_chr4_95418181_F Primer3: not found at this Tm N.d. 0.23
mm4_intergenic_Gm12708|Gm830_chr4_95418181_R Primer3: not found at this Tm N.d. 0.23
mm4_intron_Meis2_chr2_115882411_F Primer3: not found at this Tm N.d. 0.23
mm4_intron_Meis2_chr2_115882411_R Primer3: not found at this Tm N.d. 0.23
mm4_intron_Cntnap2_chr6_47156479_F Primer3: not found at this Tm N.d. 0.22
mm4_intron_Cntnap2_chr6_47156479_R Primer3: not found at this Tm N.d. 0.22
mm4_intron_Cntnap2_chr6_47137544_F TCGTCGGCAGCGTCAGAACTTTGGGTGAGATGACTC 57.7 0.22
mm4_intron_Cntnap2_chr6_47137544_R GTCTCGTGGGCTCGGCAGCATCAGAAGGAAGGGCA 60.0 0.22
mm4_intron_Cntnap2_chr6_47119116_F TCGTCGGCAGCGTCAGAGCTTTGGGTGAGATGACTC 59.7 0.22
mm4_intron_Cntnap2_chr6_47119116_R GTCTCGTGGGCTCGGTTTGGGCCAGCATCAGAAGG 60.6 0.22
mm4_intron_Rreb1_chr13_37833082_F TCGTCGGCAGCGTCAAGACAGGATGGGGAGGGAA 59.8 0.22
mm4_intron_Rreb1_chr13_37833082_R GTCTCGTGGGCTCGGGCTGGGGTCCCATACAAACA 59.9 0.22
mm4_intron_Mettl15_chr2_109205877_F TCGTCGGCAGCGTCACAAGCATTTCTTCTCTGTTCCT 58.2 0.22
mm4_intron_Mettl15_chr2_109205877_R GTCTCGTGGGCTCGGTGCTAGATATGTCTGGGACTGA 57.8 0.22
mm4_intron_Alk_chr17_72341459_F Primer3: not found at this Tm N.d. 0.22
mm4_intron_Alk_chr17_72341459_R Primer3: not found at this Tm N.d. 0.22
mm4_intergenic_Gm5316|Clec4a1_chr6_122901768_F Primer3: not found at this Tm N.d. 0.21
mm4_intergenic_Gm5316|Clec4a1_chr6_122901768_R Primer3: not found at this Tm N.d. 0.21
mm4_intron_Dusp10_chr1_184034806_F TCGTCGGCAGCGTCAGTCTTTTGGCTCTGCTTGC 59.0 0.21
mm4_intron_Dusp10_chr1_184034806_R GTCTCGTGGGCTCGGTATTCCGTGCCACCACTGAG 59.7 0.21
mm4_intron_Pld5_chr1_175987635_F TCGTCGGCAGCGTCTCATTTCCTCCTCCCTCCTT 57.3 0.19
mm4_intron_Pld5_chr1_175987635_R GTCTCGTGGGCTCGGTGGACATGACTGAAATGGTGA 57.2 0.19
mm3_intergenic_Gm16087|Gm16086_chr12_107027851_F TCGTCGGCAGCGTCAGACAGAGGGACAGACGCTA 59.6 0.19
mm3_intergenic_Gm16087|Gm16086_chr12_107027851_R GTCTCGTGGGCTCGGCTCTCTGAAGTTACCCCGCC 59.8 0.19
mm4_intergenic_4933402E13Rik|Gm14679_chrX_62338517_F Primer3: not found at this Tm N.d. 0.18
mm4_intergenic_4933402E13Rik|Gm14679_chrX_62338517_R Primer3: not found at this Tm N.d. 0.18
mm4_intergenic_Pds5a|1700022K14Rik_chr5_65717919_F Primer3: not found at this Tm N.d. 0.18
mm4_intergenic_Pds5a|1700022K14Rik_chr5_65717919_R Primer3: not found at this Tm N.d. 0.18
mm4_intergenic_Tmem189|Gm22355_chr2_167644223_F TCGTCGGCAGCGTCGCCTGCAGCAGACACAGTTA 60.6 0.18
mm4_intergenic_Tmem189|Gm22355_chr2_167644223_R GTCTCGTGGGCTCGGAAGCCGCACTTGAAAGAGGT 60.1 0.18
mm4_exon_Tulp2_chr7_45520993_F TCGTCGGCAGCGTCGAAAGCACAGGGTCAGACCA 59.8 0.18
mm4_exon_Tulp2_chr7_45520993_R GTCTCGTGGGCTCGGTGTGGCTGGACTTTCATCCTC 59.9 0.18
mm4_intron_Arpc2_chr1_74267681_F Primer3: not found at this Tm N.d. 0.17
mm4_intron_Arpc2_chr1_74267681_R Primer3: not found at this Tm N.d. 0.17
mm4_intergenic_5430427O19Rik|Gm14763_chrX_85892500_F Primer3: not found at this Tm N.d. 0.17
mm4_intergenic_5430427O19Rik|Gm14763_chrX_85892500_R Primer3: not found at this Tm N.d. 0.17
mm4_intron_Col4a3bp_chr13_96556294_F Primer3: not found at this Tm N.d. 0.16
mm4_intron_Col4a3bp_chr13_96556294_R Primer3: not found at this Tm N.d. 0.16
mm4_intergenic_Asphd2|Sez6l_chr5_112392254_F TCGTCGGCAGCGTCTCAGTCACCGTGAAAGGGTT 59.1 0.16
mm4_intergenic_Asphd2|Sez6l_chr5_112392254_R GTCTCGTGGGCTCGGGCTGGATCTCCATCTTCCCG 59.9 0.16
mm4_intron_St8sia3_chr18_64257136_F Primer3: not found at this Tm N.d. 0.16
mm4_intron_St8sia3_chr18_64257136_R Primer3: not found at this Tm N.d. 0.16
mm4_intron_Mad1l1_chr5_140044611_F TCGTCGGCAGCGTCCCACATGGGGCTCTCAGAAT 59.4 0.16
mm4_intron_Mad1l1_chr5_140044611_R GTCTCGTGGGCTCGGTACTGACCTCTGCGACTCCA 59.9 0.16
mm4_intron_Elfn1_chr5_139913296_F TCGTCGGCAGCGTCTCCCCATAAACTTGGAGCGG 59.7 0.15
mm4_intron_Elfn1_chr5_139913296_R GTCTCGTGGGCTCGGTAAACACAGCCTGGGACACC 59.8 0.15
mm4_intron_Lag3_chr6_124904993_F Primer3: not found at this Tm N.d. 0.15
mm4_intron_Lag3_chr6_124904993_R Primer3: not found at this Tm N.d. 0.15
mm4_intergenic_Gna12|Card11_chr5_140871016_F Primer3: not found at this Tm N.d. 0.15
mm4_intergenic_Gna12|Card11_chr5_140871016_R Primer3: not found at this Tm N.d. 0.15
mm3_intergenic_Gm25226|Plxnb3_chrX_73756759_F Primer3: not found at this Tm N.d. 0.15
mm3_intergenic_Gm25226|Plxnb3_chrX_73756759_R Primer3: not found at this Tm N.d. 0.15
mm4_intergenic_Gm15074|Gm4996_chrX_146791936_F Primer3: not found at this Tm N.d. 0.15
mm4_intergenic_Gm15074|Gm4996_chrX_146791936_R Primer3: not found at this Tm N.d. 0.15
mm4_intron_Golm1_chr13_59674221_F TCGTCGGCAGCGTCTGAAAAGACCAAAGCCCTGC 58.9 0.14
mm4_intron_Golm1_chr13_59674221_R GTCTCGTGGGCTCGGAGTTGGAGGACAGAGGGAGG 60.2 0.14
mm4_intron_Tenm4_chr7_96468903_F TCGTCGGCAGCGTCAAGGTGTTGGCCTTGGCAA 60.3 0.14
mm4_intron_Tenm4_chr7_96468903_R GTCTCGTGGGCTCGGCCTCTCCCCACCCCTAAGTT 60.2 0.14
mm4_intergenic_Gm13855|Gm14546_chr6_34253093_F TCGTCGGCAGCGTCCAAGCCCTCAACGACAGCTA 60.0 0.14
mm4_intergenic_Gm13855|Gm14546_chr6_34253093_R GTCTCGTGGGCTCGGCACATTGAGTGTTGGTATGGCT 58.9 0.14
mm4_intergenic_Gm22777|Spock1_chr13_57139054_F TCGTCGGCAGCGTCGCCGTGAGTCACATCCAAGA 60.0 0.14
mm4_intergenic_Gm22777|Spock1_chr13_57139054_R GTCTCGTGGGCTCGGGGATGAACTGGGAGGCTGTG 60.3 0.14
mm4_intergenic_Gm6434|Cyp2b10_chr7_25895903_F Primer3: not found at this Tm N.d. 0.13
mm4_intergenic_Gm6434|Cyp2b10_chr7_25895903_R Primer3: not found at this Tm N.d. 0.13
mm4_intron_Ksr2_chr5_117576372_F TCGTCGGCAGCGTCGCTGTTTCTCAACTGGCCTT 58.6 0.13
mm4_intron_Ksr2_chr5_117576372_R GTCTCGTGGGCTCGGAAGGTAGAAGCCTTCCTGGG 58.7 0.13
mm4_intron_Epha10_chr4_124914650_F TCGTCGGCAGCGTCATCCCGCCTAGAGTCCCTG 60.1 0.13
mm4_intron_Epha10_chr4_124914650_R GTCTCGTGGGCTCGGCATCCTCCACGGCCTTGATT 60.1 0.13
mm3_intron_Rgs9_chr11_109246171_F Primer3: not found at this Tm N.d. 0.12
mm3_intron_Rgs9_chr11_109246171_R Primer3: not found at this Tm N.d. 0.12
mm4_intron_Spsb4_chr9_96987464_F Primer3: not found at this Tm N.d. 0.12
mm4_intron_Spsb4_chr9_96987464_R Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_H2-T3|Gm4246_chr17_36192055_F Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_H2-T3|Gm4246_chr17_36192055_R Primer3: not found at this Tm N.d. 0.12
mm3_intergenic_Rell1|Gm25608_chr5_63969227_F TCGTCGGCAGCGTCTGGGTCCTGGGACTAGTCAG 59.9 0.12
mm3_intergenic_Rell1|Gm25608_chr5_63969227_R GTCTCGTGGGCTCGGCGCCATTCTGAAACTGCTGG 59.8 0.12
mm4_intergenic_Gm22848|Rp1_chr1_4072125_F Primer3: not found at this Tm N.d. 0.11
mm4_intergenic_Gm22848|Rp1_chr1_4072125_R Primer3: not found at this Tm N.d. 0.11
mm4_intron_Fam53b_chr7_132786607_F Primer3: not found at this Tm N.d. 0.11
mm4_intron_Fam53b_chr7_132786607_R Primer3: not found at this Tm N.d. 0.11
mm4_intron_Ppfia2_chr10_106738410_F TCGTCGGCAGCGTCGGATCCTGACAGCTGCCATT 60.1 0.11
mm4_intron_Ppfia2_chr10_106738410_R GTCTCGTGGGCTCGGTGGGTCTGTGACACTCGATG 59.3 0.11
mm4_intergenic_Gm25491|Eya1_chr1_14163370_F TCGTCGGCAGCGTCGACTAAGATGCCTGCCCCTC 59.8 0.10
mm4_intergenic_Gm25491|Eya1_chr1_14163370_R GTCTCGTGGGCTCGGAGAGCTTGTCCAAGGGAAGC 59.9 0.10
mm4_intergenic_Fgf9|Gm17109_chr14_58087790_F Primer3: not found at this Tm N.d. 0.10
mm4_intergenic_Fgf9|Gm17109_chr14_58087790_R Primer3: not found at this Tm N.d. 0.10
mm4_intron_Cacna1h_chr17_25427166_F Primer3: not found at this Tm N.d. 0.09
mm4_intron_Cacna1h_chr17_25427166_R Primer3: not found at this Tm N.d. 0.09
mm4_intergenic_Gm22845|Nt5c1b_chr12_10070541_F TCGTCGGCAGCGTCACACTTGGAAATGGCCTCCA 59.5 0.09
mm4_intergenic_Gm22845|Nt5c1b_chr12_10070541_R GTCTCGTGGGCTCGGTGTGCTATGACTGTGGAGGG 59.0 0.09
mm4_intergenic_9030612E09Rik|Pdss2_chr10_43214980_F TCGTCGGCAGCGTCGGGATACACACAGTATTACATAGCA 58.2 0.09
mm4_intergenic_9030612E09Rik|Pdss2_chr10_43214980_R GTCTCGTGGGCTCGGGCCCACAAGATCATTCTCAATTCT 59.3 0.09
mm3_intergenic_Acox3|Htra3_chr5_35622138_F TCGTCGGCAGCGTCGGGCCTCAGTGAACCATACA 59.3 0.08
mm3_intergenic_Acox3|Htra3_chr5_35622138_R GTCTCGTGGGCTCGGTGTGCTCAGGCTTTGGTGTA 59.5 0.08
mm4_intergenic_mmu-mir-6378|Gm25442_chr3_35066689_F TCGTCGGCAGCGTCGGAAAGGAAAGAGGCCGCTT 60.6 0.08
mm4_intergenic_mmu-mir-6378|Gm25442_chr3_35066689_R GTCTCGTGGGCTCGGTGGGTGGGTTGTCCTGTAAA 58.7 0.08
mm3_intron_Dscam_chr16_96736513_F TCGTCGGCAGCGTCGGCTGCAAGAAAAGCATCCC 60.1 0.08
mm3_intron_Dscam_chr16_96736513_R GTCTCGTGGGCTCGGGTGGGTCAGCACTTTCATGC 59.7 0.08
mm4_intergenic_3110083C13Rik|Fgf9_chr14_58061738_F Primer3: not found at this Tm N.d. 0.08
mm4_intergenic_3110083C13Rik|Fgf9_chr14_58061738_R Primer3: not found at this Tm N.d. 0.08
mm4_intron_Iqca_chr1_90109688_F Primer3: not found at this Tm N.d. 0.08
mm4_intron_Iqca_chr1_90109688_R Primer3: not found at this Tm N.d. 0.08
mm4_intergenic_Rnf144b|4931429P17Rik_chr13_47636421_F TCGTCGGCAGCGTCAGGACTCAAGAGAAACCCCA 57.8 0.07
mm4_intergenic_Rnf144b|4931429P17Rik_chr13_47636421_R GTCTCGTGGGCTCGGCTCATTAGGTCCAGGTCCCC 58.8 0.07
mm4_intergenic_1700109G15Rik|Gm11431_chr11_84760809_F Primer3: not found at this Tm N.d. 0.07
mm4_intergenic_1700109G15Rik|Gm11431_chr11_84760809_R Primer3: not found at this Tm N.d. 0.07
mm4_intron_Zfpm2_chr15_41056745_F Primer3: not found at this Tm N.d. 0.06
mm4_intron_Zfpm2_chr15_41056745_R Primer3: not found at this Tm N.d. 0.06
mm4_intergenic_Rora|F830001A07Rik_chr9_68775566_F TCGTCGGCAGCGTCGTAGAAACGCTGGGCTCTCC 60.4 0.06
mm4_intergenic_Rora|F830001A07Rik_chr9_68775566_R GTCTCGTGGGCTCGGGGGGTTTGGGAACTGGATGT 59.8 0.06
mm4_intron_Rab27b_chr18_70094936_F Primer3: not found at this Tm N.d. 0.06
mm4_intron_Rab27b_chr18_70094936_R Primer3: not found at this Tm N.d. 0.06
mm4_intron_Usp25_chr16_77054625_F Primer3: not found at this Tm N.d. 0.06
mm4_intron_Usp25_chr16_77054625_R Primer3: not found at this Tm N.d. 0.06
mm4_intron_Gse1_chr8_120304608_F TCGTCGGCAGCGTCAGTGACCAAACCCTCTGCAG 59.8 0.06
mm4_intron_Gse1_chr8_120304608_R GTCTCGTGGGCTCGGCGGCCCATTCACAAAACAGG 60.0 0.06
mm4_intron_Igf2bp2_chr16_22136732_F Primer3: not found at this Tm N.d. 0.06
mm4_intron_Igf2bp2_chr16_22136732_R Primer3: not found at this Tm N.d. 0.06
mm4_intron_St6galnac3_chr3_153463705_F Primer3: not found at this Tm N.d. 0.06
mm4_intron_St6galnac3_chr3_153463705_R Primer3: not found at this Tm N.d. 0.06
mm3_intergenic_Vamp8|Ggcx_chr6_72409838_F Primer3: not found at this Tm N.d. 0.06
mm3_intergenic_Vamp8|Ggcx_chr6_72409838_R Primer3: not found at this Tm N.d. 0.06
mm4_intergenic_Rxfp3|Adamts12_chr15_11063185_F TCGTCGGCAGCGTCGCAAAACACAGGAGCCAGTG 59.9 0.05
mm4_intergenic_Rxfp3|Adamts12_chr15_11063185_R GTCTCGTGGGCTCGGCAGGGACCCAAAATGCAAGG 59.3 0.05
mm4_intron_Hagh_chr17_24857839_F Primer3: not found at this Tm N.d. 0.05
mm4_intron_Hagh_chr17_24857839_R Primer3: not found at this Tm N.d. 0.05
mm4_intron_Apba1_chr19_23923067_F Primer3: not found at this Tm N.d. 0.05
mm4_intron_Apba1_chr19_23923067_R Primer3: not found at this Tm N.d. 0.05
mm3_intron_Slc6a1_chr6_114305034_F Primer3: not found at this Tm N.d. 0.05
mm3_intron_Slc6a1_chr6_114305034_R Primer3: not found at this Tm N.d. 0.05
mm4_intron_Rimbp2_chr5_128832917_F Primer3: not found at this Tm N.d. 0.05
mm4_intron_Rimbp2_chr5_128832917_R Primer3: not found at this Tm N.d. 0.05
mm3_intron_Sox5_chr6_143936789_F Primer3: not found at this Tm N.d. 0.05
mm3_intron_Sox5_chr6_143936789_R Primer3: not found at this Tm N.d. 0.05
mm3_intergenic_5031439G07Rik|Upk3a_chr15_85000157_F Primer3: not found at this Tm N.d. 0.05
mm3_intergenic_5031439G07Rik|Upk3a_chr15_85000157_R Primer3: not found at this Tm N.d. 0.05
mm4_intergenic_Gm23591|Speer2_chr16_69482569_F Primer3: not found at this Tm N.d. 0.04
mm4_intergenic_Gm23591|Speer2_chr16_69482569_R Primer3: not found at this Tm N.d. 0.04
mm3_intergenic_Olfr972|Olfr229_chr9_39904218_F Primer3: not found at this Tm N.d. 0.04
mm3_intergenic_Olfr972|Olfr229_chr9_39904218_R Primer3: not found at this Tm N.d. 0.04
mm4_intron_R3hdm1_chr1_128131647_F TCGTCGGCAGCGTCACCTGGAACTTGGGGAGACT 60.1 0.04
mm4_intron_R3hdm1_chr1_128131647_R GTCTCGTGGGCTCGGTCACTGTTCCAACTCACTGTCT 59.2 0.04
mm4_intergenic_Gprc5b|Gpr139_chr7_119023582_F Primer3: not found at this Tm N.d. 0.03
mm4_intergenic_Gprc5b|Gpr139_chr7_119023582_R Primer3: not found at this Tm N.d. 0.03
mm4_intergenic_Gm25736|Tmeff2_chr1_51029470_F TCGTCGGCAGCGTCAGAGCTGGATATATGGCTCCA 57.8 0.03
mm4_intergenic_Gm25736|Tmeff2_chr1_51029470_R GTCTCGTGGGCTCGGAGTGAGTTTTCCTCCCTGCC 59.5 0.03
mm4_intron_Frmd4a_chr2_4414130_F TCGTCGGCAGCGTCTGTTCTCACTGGTGCCATGG 60.2 0.03
mm4_intron_Frmd4a_chr2_4414130_R GTCTCGTGGGCTCGGGGCTGCCATGATAGACTGCA 60.1 0.03
mm4_intergenic_Gm26430|Gm23895_chr18_78622505_F Primer3: not found at this Tm N.d. 0.03
mm4_intergenic_Gm26430|Gm23895_chr18_78622505_R Primer3: not found at this Tm N.d. 0.03
mm3_intergenic_Chrm1/Gm22820|Slc3a2_chr19_8695728_F TCGTCGGCAGCGTCTGTCCTTGCTCTGCTTCCTG 59.9 0.03
mm3_intergenic_Chrm1/Gm22820|Slc3a2_chr19_8695728_R GTCTCGTGGGCTCGGTTCCACATAGGGACTTGGGC 59.3 0.03
mm4_intergenic_Tec|Gm9870/Slain2_chr5_72899246_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Tec|Gm9870/Slain2_chr5_72899246_R Primer3: not found at this Tm N.d. 0.02
mm4_intron_Slc25a30_chr14_75772694_F Primer3: not found at this Tm N.d. 0.02
mm4_intron_Slc25a30_chr14_75772694_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_9230111E07Rik|1200007C13Rik_chr2_167790512_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_9230111E07Rik|1200007C13Rik_chr2_167790512_R Primer3: not found at this Tm N.d. 0.02
mm3_intergenic_Gm25799|Slit3_chr11_34971500_F Primer3: not found at this Tm N.d. 0.02
mm3_intergenic_Gm25799|Slit3_chr11_34971500_R Primer3: not found at this Tm N.d. 0.02
mm4_intron_Ncapd2_chr6_125189713_F TCGTCGGCAGCGTCGGCAGGGACTAGGAAAGACC 59.4 0.02
mm4_intron_Ncapd2_chr6_125189713_R GTCTCGTGGGCTCGGCGTCAAGCACCTCTCATCCC 60.4 0.02
mm4_exon_Tmem116_chr5_121495258_F Primer3: not found at this Tm N.d. 0.02
mm4_exon_Tmem116_chr5_121495258_R Primer3: not found at this Tm N.d. 0.02
mm4_intron_Lrig3_chr10_126004204_F Primer3: not found at this Tm N.d. 0.02
mm4_intron_Lrig3_chr10_126004204_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_4933439C10Rik|Zkscan17_chr11_59521539_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_4933439C10Rik|Zkscan17_chr11_59521539_R Primer3: not found at this Tm N.d. 0.02
mm3_intergenic_Gm5611|Chordc1_chr9_18232752_F Primer3: not found at this Tm N.d. 0.02
mm3_intergenic_Gm5611|Chordc1_chr9_18232752_R Primer3: not found at this Tm N.d. 0.02
mm4_intron_Atm_chr9_53476837_F TCGTCGGCAGCGTCTCCATCCGAGGTGGCTAGTA 59.4 0.01
mm4_intron_Atm_chr9_53476837_R GTCTCGTGGGCTCGGTGAATGCACATCCTGCCAGT 59.9 0.01
mm4_intergenic_Gm5908|Gm3985_chr8_32876295_F Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Gm5908|Gm3985_chr8_32876295_R Primer3: not found at this Tm N.d. 0.01
mm3_intron_Ccl1_chr11_82254769_F TCGTCGGCAGCGTCACCTGACTGAGATTGGCAGC 60.0 0.01
mm3_intron_Ccl1_chr11_82254769_R GTCTCGTGGGCTCGGGTTCCACAGGCCACACTTCT 60.1 0.01
mm4_intergenic_Chrm2|Gm25111_chr6_36603850_F Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Chrm2|Gm25111_chr6_36603850_R Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Rims4|Ywhab_chr2_163953362_F Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Rims4|Ywhab_chr2_163953362_R Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Eif4g2|Galnt18_chr7_111368991_F Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Eif4g2|Galnt18_chr7_111368991_R Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Pou3f1|Utp11l_chr4_124664679_F TCGTCGGCAGCGTCCCCACATGGAAAGAGGGCTT 59.9 0.01
mm4_intergenic_Pou3f1|Utp11l_chr4_124664679_R GTCTCGTGGGCTCGGATCGGGTGTGAGAGAGCAAC 59.7 0.01
mm3_intergenic_Arhgef39|Gm12454_chr4_43503634_F TCGTCGGCAGCGTCCCCTCAAGCGCCAGTTAAGA 60.0 0.01
mm3_intergenic_Arhgef39|Gm12454_chr4_43503634_R GTCTCGTGGGCTCGGAACTGGATGGGCGTGTCAAA 60.1 0.01
mm4_intergenic_Rnu1b6|5S_rRNA_chr3_96460453_F TCGTCGGCAGCGTCCCACCCAGGAGTGACATCAC 60.0 0.00
mm4_intergenic_Rnu1b6|5S_rRNA_chr3_96460453_R GTCTCGTGGGCTCGGTAGGGGTAAGAGACGGTGCA 59.9 0.00
mm4_intergenic_Fcgr1|Gm24830_chr3_96328137_F TCGTCGGCAGCGTCTGCGCAAGTGAGAAAGTCCA 60.1 0.00
mm4_intergenic_Fcgr1|Gm24830_chr3_96328137_R GTCTCGTGGGCTCGGCCACCCAGGAGTGACATCAC 60.0 0.00
mm4_intergenic_BC107364|Gm26232_chr3_96450158_F TCGTCGGCAGCGTCTAGGGGTAAGAGACGGTGCA 59.9 0.00
mm4_intergenic_BC107364|Gm26232_chr3_96450158_R GTCTCGTGGGCTCGGCCACCCAGGAGTGACATCAC 60.0 0.00
mm4_intergenic_Gm16968|Adam6a_chr12_113528554_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm16968|Adam6a_chr12_113528554_R Primer3: not found at this Tm N.d. 0.00
mm4_intron_Dapk1_chr13_60695169_F TCGTCGGCAGCGTCTGTCTCAGTACCTCCGAGGG 60.0 0.00
mm4_intron_Dapk1_chr13_60695169_R GTCTCGTGGGCTCGGGAACACACAACCAGATCTCATTC 57.7 0.00
mm4_intergenic_Tex16|Gm6335_chrX_112162509_F TCGTCGGCAGCGTCTATACCGGGAGCAGCATGTG 59.6 0.00
mm4_intergenic_Tex16|Gm6335_chrX_112162509_R GTCTCGTGGGCTCGGTGGCATTGGATTTGACTCCCA 59.9 0.00
mm4_intergenic_Psmd13|Gm15542_chr7_140890842_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Psmd13|Gm15542_chr7_140890842_R Primer3: not found at this Tm N.d. 0.00
mm4_exon_Dnaja1_chr4_40722599_F TCGTCGGCAGCGTCAACTTTCCAGAACGCTCGGT 59.8 0.00
mm4_exon_Dnaja1_chr4_40722599_R GTCTCGTGGGCTCGGGAGGGAGGCCGGAGACTC 60.8 0.00
mm4_intron_Trpa1_chr1_14877290_F Primer3: not found at this Tm N.d. 0.00
mm4_intron_Trpa1_chr1_14877290_R Primer3: not found at this Tm N.d. 0.00
mm4_intron_Tbl1xr1_chr3_22144884_F TCGTCGGCAGCGTCGGCCAAGGTAGAGGAGAAGC 59.8 0.00
mm4_intron_Tbl1xr1_chr3_22144884_R GTCTCGTGGGCTCGGCCTGCACATCATCTGCCCTT 60.3 0.00
mm4_intron_Spats2l_chr1_57783095_F Primer3: not found at this Tm N.d. 0.00
mm4_intron_Spats2l_chr1_57783095_R Primer3: not found at this Tm N.d. 0.00
mm4_intron_Sh3gl3_chr7_82213127_F Primer3: not found at this Tm N.d. 0.00
mm4_intron_Sh3gl3_chr7_82213127_R Primer3: not found at this Tm N.d. 0.00
mm4_intron_Msh2_chr17_87686849_F Primer3: not found at this Tm N.d. 0.00
mm4_intron_Msh2_chr17_87686849_R Primer3: not found at this Tm N.d. 0.00
mm4_intron_Mcat_chr15_83548124_F Primer3: not found at this Tm N.d. 0.00
mm4_intron_Mcat_chr15_83548124_R Primer3: not found at this Tm N.d. 0.00
mm4_intron_Grin2a_chr16_9784088_F Primer3: not found at this Tm N.d. 0.00
mm4_intron_Grin2a_chr16_9784088_R Primer3: not found at this Tm N.d. 0.00
mm4_intron_Greb1_chr12_16750158_F Primer3: not found at this Tm N.d. 0.00
mm4_intron_Greb1_chr12_16750158_R Primer3: not found at this Tm N.d. 0.00
mm4_intron_Gm14040_chr2_129515346_F TCGTCGGCAGCGTCAATTCTGTCTCGGCAAAGCC 58.8 0.00
mm4_intron_Gm14040_chr2_129515346_R GTCTCGTGGGCTCGGGCTGGGGCTGAGTAAACCAA 60.2 0.00
mm4_intron_Gm12160_chr11_45175291_F TCGTCGGCAGCGTCACCACTGAACATCTGCTGGG 59.9 0.00
mm4_intron_Gm12160_chr11_45175291_R GTCTCGTGGGCTCGGCACTTTAAGGGAAGCCACGC 59.4 0.00
mm4_intron_Filip1_chr9_79828428_F TCGTCGGCAGCGTCTTTGCCACCCTGATGCATCC 60.9 0.00
mm4_intron_Filip1_chr9_79828428_R GTCTCGTGGGCTCGGGAGGGACCGAGGTGAAAGTG 60.0 0.00
mm4_intron_Fam131c_chr4_141371465_F Primer3: not found at this Tm N.d. 0.00
mm4_intron_Fam131c_chr4_141371465_R Primer3: not found at this Tm N.d. 0.00
mm4_intron_Dcc_chr18_71623072_F Primer3: not found at this Tm N.d. 0.00
mm4_intron_Dcc_chr18_71623072_R Primer3: not found at this Tm N.d. 0.00
mm4_intron_Cntn4_chr6_106193413_F TCGTCGGCAGCGTCCCATGCCCTGTTTCTCCCTT 59.9 0.00
mm4_intron_Cntn4_chr6_106193413_R GTCTCGTGGGCTCGGGTGACCTCTCTATGGTGCCA 58.8 0.00
mm4_intron_Chm_chrX_113185313_F Primer3: not found at this Tm N.d. 0.00
mm4_intron_Chm_chrX_113185313_R Primer3: not found at this Tm N.d. 0.00
mm4_intron_Casz1_chr4_148821083_F Primer3: not found at this Tm N.d. 0.00
mm4_intron_Casz1_chr4_148821083_R Primer3: not found at this Tm N.d. 0.00
mm4_intron_Bcor_chrX_12081883_F Primer3: not found at this Tm N.d. 0.00
mm4_intron_Bcor_chrX_12081883_R Primer3: not found at this Tm N.d. 0.00
mm4_intron_Batf2_chr19_6161622_F TCGTCGGCAGCGTCGACTGGGAGCTTGGACGAAA 59.9 0.00
mm4_intron_Batf2_chr19_6161622_R GTCTCGTGGGCTCGGCATTCCTAAAGGGTGGGGTCA 59.3 0.00
mm4_intron_Atp6v0a2_chr5_124711714_F TCGTCGGCAGCGTCCCAGGGATCACAGCTCCATC 59.8 0.00
mm4_intron_Atp6v0a2_chr5_124711714_R GTCTCGTGGGCTCGGGGATGGAACTGTGACCCCTG 60.0 0.00
mm4_intron_Atp6v0a2_chr5_124711682_F TCGTCGGCAGCGTCTCTCCTTCCCAGGGATCACA 59.5 0.00
mm4_intron_Atp6v0a2_chr5_124711682_R GTCTCGTGGGCTCGGGATGGAACTGTGACCCCTGG 60.0 0.00
mm4_intron_Atp6v0a2_chr5_124711650_F Primer3: not found at this Tm N.d. 0.00
mm4_intron_Atp6v0a2_chr5_124711650_R Primer3: not found at this Tm N.d. 0.00
mm4_intron_Atp6v0a2_chr5_124711618_F TCGTCGGCAGCGTCTCTCTTTCCCAGGGGTCACA 60.1 0.00
mm4_intron_Atp6v0a2_chr5_124711618_R GTCTCGTGGGCTCGGGATGGAGCTGTGATCCCTGG 59.8 0.00
mm4_intron_Atp6v0a2_chr5_124711586_F TCGTCGGCAGCGTCTCTCTTTCCCAGGGGTCACA 60.1 0.00
mm4_intron_Atp6v0a2_chr5_124711586_R GTCTCGTGGGCTCGGGAACTGTGACCCCTGGGAAG 59.9 0.00
mm4_intron_Atp6v0a2_chr5_124711522_F Primer3: not found at this Tm N.d. 0.00
mm4_intron_Atp6v0a2_chr5_124711522_R Primer3: not found at this Tm N.d. 0.00
mm4_intron_Atp6v0a2_chr5_124711490_F TCGTCGGCAGCGTCCAGGGGTCACAGTTCCATCC 60.0 0.00
mm4_intron_Atp6v0a2_chr5_124711490_R GTCTCGTGGGCTCGGTGTGACCCCTGGGAAAGAGA 60.1 0.00
mm4_intron_Atp6v0a2_chr5_124711458_F TCGTCGGCAGCGTCCATCCTCACTTCCCAGCTCC 59.8 0.00
mm4_intron_Atp6v0a2_chr5_124711458_R GTCTCGTGGGCTCGGTGACCCCTGGGAAGGAGAG 59.9 0.00
mm4_intron_Ankrd46_chr15_36487485_F Primer3: not found at this Tm N.d. 0.00
mm4_intron_Ankrd46_chr15_36487485_R Primer3: not found at this Tm N.d. 0.00
mm4_intron_Amfr_chr8_93979122_F TCGTCGGCAGCGTCGGAAAGAAGGAGCCCGAACA 59.9 0.00
mm4_intron_Amfr_chr8_93979122_R GTCTCGTGGGCTCGGGGGAACTTGGGAAGGGTTTG 58.6 0.00
mm4_intron_Agbl4_chr4_110841893_F Primer3: not found at this Tm N.d. 0.00
mm4_intron_Agbl4_chr4_110841893_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_n-R5s175|Gm2301_chr5_119313591_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_n-R5s175|Gm2301_chr5_119313591_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Zfp516|Gm26258_chr18_82958197_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Zfp516|Gm26258_chr18_82958197_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Urb2|Galnt2_chr8_124080542_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Urb2|Galnt2_chr8_124080542_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Tasp1|Rps19-ps7_chr2_140070406_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Tasp1|Rps19-ps7_chr2_140070406_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Tada3|Arpc4_chr6_113378056_F TCGTCGGCAGCGTCACTCGACCGCTACTGTCTCA 60.3 0.00
mm4_intergenic_Tada3|Arpc4_chr6_113378056_R GTCTCGTGGGCTCGGCTGGACCATCTCGGGTAGGA 60.1 0.00
mm4_intergenic_Rps6-ps2|mmu-mir-8110_chr8_88925683_F TCGTCGGCAGCGTCCACCCAGCCACTTGTCTCTT 59.8 0.00
mm4_intergenic_Rps6-ps2|mmu-mir-8110_chr8_88925683_R GTCTCGTGGGCTCGGAGCACATCACCCAGGAAGTT 59.2 0.00
mm4_intergenic_Loxl4|Gm25216_chr19_42692212_F TCGTCGGCAGCGTCTAGAGGCATGCTCTTGCTGG 59.8 0.00
mm4_intergenic_Loxl4|Gm25216_chr19_42692212_R GTCTCGTGGGCTCGGGGGTCCCTGTGAACAAACCA 60.1 0.00
mm4_intergenic_Kirrel3|Gm23702_chr9_34947581_F TCGTCGGCAGCGTCAGGCAAGAACTGACTCTGAGC 59.9 0.00
mm4_intergenic_Kirrel3|Gm23702_chr9_34947581_R GTCTCGTGGGCTCGGGGGGTGCTCAGCTTCTTAGG 60.1 0.00
mm4_intergenic_Junb|Hook2_chr8_84987273_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Junb|Hook2_chr8_84987273_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Itgb4|B230344G16Rik/Itgb4_chr11_115987545_F TCGTCGGCAGCGTCCCCCTGTGTGGCTTTGTCTA 59.6 0.00
mm4_intergenic_Itgb4|B230344G16Rik/Itgb4_chr11_115987545_R GTCTCGTGGGCTCGGCTCGCTCACACCTCCATCTC 59.8 0.00
mm4_intergenic_Gm7488|Tbl1xr1_chr3_21995190_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm7488|Tbl1xr1_chr3_21995190_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm6245|Gcnt2_chr13_40796537_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm6245|Gcnt2_chr13_40796537_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm5274|Gm23891_chr3_47166223_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm5274|Gm23891_chr3_47166223_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm26878|Fendrr_chr8_120927558_F TCGTCGGCAGCGTCTCCCGTATTCCTCTCTTCCCA 59.7 0.00
mm4_intergenic_Gm26878|Fendrr_chr8_120927558_R GTCTCGTGGGCTCGGCCACCACCCTAGCAGTGAAG 60.0 0.00
mm4_intergenic_Gm26480|Gm6863_chr12_96308239_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm26480|Gm6863_chr12_96308239_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm26228|Gdf10_chr14_33789539_F TCGTCGGCAGCGTCAACCCTGACCTACAGCCTCT 59.8 0.00
mm4_intergenic_Gm26228|Gdf10_chr14_33789539_R GTCTCGTGGGCTCGGCAACAGCATTGCGAAGACCC 60.1 0.00
mm4_intergenic_Gm25985|Gm6404_chr13_115224861_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm25985|Gm6404_chr13_115224861_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm25839|4930587A21Rik_chr1_61513298_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm25839|4930587A21Rik_chr1_61513298_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm25264|Gm25959_chr2_47462084_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm25264|Gm25959_chr2_47462084_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm25051|Gm1818_chr12_47954362_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm25051|Gm1818_chr12_47954362_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm23666|U7_chr8_53026720_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm23666|U7_chr8_53026720_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm23314|Lingo2_chr4_36855168_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm23314|Lingo2_chr4_36855168_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm22914|Skap2_chr6_51782698_F TCGTCGGCAGCGTCCGGGGTCACTGGTTCTCTTC 60.0 0.00
mm4_intergenic_Gm22914|Skap2_chr6_51782698_R GTCTCGTGGGCTCGGGTCTTCCTCAAAGGGGCTCC 60.0 0.00
mm4_intergenic_Gm22438|Gm22159_chr1_109393847_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm22438|Gm22159_chr1_109393847_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm17054|n-R5s37_chr16_61132857_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm17054|n-R5s37_chr16_61132857_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm15756/Aldh1l1|Aldh1l1_chr6_90597133_F TCGTCGGCAGCGTCAGGAGGAGACTGAGTCAGCA 59.5 0.00
mm4_intergenic_Gm15756/Aldh1l1|Aldh1l1_chr6_90597133_R GTCTCGTGGGCTCGGTTTTGCTCATCTGGGCCTGG 60.6 0.00
mm4_intergenic_Gm14920|Gm14918_chrX_117237814_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm14920|Gm14918_chrX_117237814_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm14318|B230312C02Rik_chr2_180352653_F TCGTCGGCAGCGTCGCATCCTCACAAGGTGGGAA 59.9 0.00
mm4_intergenic_Gm14318|B230312C02Rik_chr2_180352653_R GTCTCGTGGGCTCGGAGGAGGATGTGGTACTGGCA 60.2 0.00
mm4_intergenic_Gm13323|H2afb1_chr2_17865943_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm13323|H2afb1_chr2_17865943_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm12450|Spag17_chr3_99806993_F TCGTCGGCAGCGTCGGAAGTCTTCATTCCGCCCA 60.0 0.00
mm4_intergenic_Gm12450|Spag17_chr3_99806993_R GTCTCGTGGGCTCGGTCAACGTAGAAGACTGTTTTCCT 57.7 0.00
mm4_intergenic_Gm10398|Gm26660_chr14_24978689_F TCGTCGGCAGCGTCGTGTGACTCTGTGCGTCCTT 60.2 0.00
mm4_intergenic_Gm10398|Gm26660_chr14_24978689_R GTCTCGTGGGCTCGGAAGGGTGTGCAGAGCTCTTC 59.9 0.00
mm4_intergenic_Fbxo2|Ptchd2_chr4_148173939_F TCGTCGGCAGCGTCAGTATCGGGCTGCGTTTGAA 60.0 0.00
mm4_intergenic_Fbxo2|Ptchd2_chr4_148173939_R GTCTCGTGGGCTCGGTCGGCGAGCAGAGTAATTGG 60.1 0.00
mm4_intergenic_Elmo1|Gm7611_chr13_20680589_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Elmo1|Gm7611_chr13_20680589_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Efcab4b|Prmt8_chr6_127661089_F TCGTCGGCAGCGTCATATGCTTGAGCTTGGCCGA 59.8 0.00
mm4_intergenic_Efcab4b|Prmt8_chr6_127661089_R GTCTCGTGGGCTCGGTAGGTCCATTCTCCCAGGGG 60.0 0.00
mm4_intergenic_E230025N22Rik|Cd14_chr18_36698218_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_E230025N22Rik|Cd14_chr18_36698218_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Cdk19|Slc22a16_chr10_40568159_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Cdk19|Slc22a16_chr10_40568159_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Agbl3|Gm13864_chr6_34785928_F TCGTCGGCAGCGTCTGTGTCAGAGCACACGTGAA 59.8 0.00
mm4_intergenic_Agbl3|Gm13864_chr6_34785928_R GTCTCGTGGGCTCGGAGGCAGAGACAAAGGGATTGA 59.0 0.00
mm4_intergenic_Adss|Gm16432_chr1_177802134_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Adss|Gm16432_chr1_177802134_R Primer3: not found at this Tm N.d. 0.00
mm4_exon_Frmd4a_chr2_4401119_F TCGTCGGCAGCGTCCGCTAGGGAGGCAGAAGATC 59.6 0.00
mm4_exon_Frmd4a_chr2_4401119_R GTCTCGTGGGCTCGGGCTCGATCGCTTGCGAAG 59.3 0.00
mm4_exon_Dnm3_chr1_162134565_F TCGTCGGCAGCGTCGCTGGACATGAAGCCCTTCT 60.0 0.00
mm4_exon_Dnm3_chr1_162134565_R GTCTCGTGGGCTCGGGGTGTGTTGGTTGCCATTGG 60.2 0.00
mm3_intron_Cib4_chr5_30540205_F TCGTCGGCAGCGTCCCGTTGCCTTCCTTGAGCTT 60.8 0.00
mm3_intron_Cib4_chr5_30540205_R GTCTCGTGGGCTCGGGGGTATACACATATCTGAGACAGCT 59.5 0.00
mm3_intergenic_4930564D02Rik|1700095B22Rik_chr3_105268394_F TCGTCGGCAGCGTCTCCATTTTCGCTCCAGTTGT 57.7 0.00
mm3_intergenic_4930564D02Rik|1700095B22Rik_chr3_105268394_R GTCTCGTGGGCTCGGGTAGAAGGCATGCACCTCCA 59.7 0.00
mm3_exon_A930016O22Rik/Ckm_chr7_19419065_F TCGTCGGCAGCGTCACTGAAGATTGCTGAACTCTCA 57.3 0.00
mm3_exon_A930016O22Rik/Ckm_chr7_19419065_R GTCTCGTGGGCTCGGAGGGGCGTTGTATACCTCCT 60.0 0.00

Off-target amplicon sequences with primers

These only list off-targets that have primers in the table above. Primers underlined, off-targets in bold.

mm3_intron_Erbb2_chr11_98415644 CCTTTCTCTTGGGCCCTCTGCAGTGCCAGGGATGGAGGGCCGGAAGGTGCAGAGATCACTAGGACATACAGGCTCATGGG
AATGATGTGGGCAGG
mm3_intron_Llgl2_chr11_115854045 GCCTGTGGCCTCTGTGAGGTTGGGGGAGTGGGAGGGGGGTCTCAGGCACTGCCAGGGATGGAGAGCCAAGAGTATCTCAG
ATGTGGAGCTCTGTCGG
mm4_intron_Gm20681_chr8_122374323 GCAAGAGTGAGAAGAGGGGACTTTGGTATTAACTCAGGCAAGAGCCAGGGATGGAGAGCATAGAGAACAGGAGGGAAGTT
CAGAATCCATCTGGAAGGCCGACTCGTTCCCCAGTTCATCTCCCCAGCCTCT
mm4_intron_Slamf8_chr1_172589020 CCAATGCAAGATAAAGGTGGAAACTTAAACAAAGGAATGGAACCACCGGGGATGGGGAGCCAGAAAGAGTAATACTGTAT
GTAGTTAGCAAACTCTAGTATTAGATTTGGTTTGAGAGTGAAGCGGGA
mm4_intergenic_Gm22184|Asprv1_chr6_86606636 AAGGTTGTCAGGGCCCAAATCAGATGCCTTTCTGTTCTCCACCCCAGGTAAGCACAAGGGTGACTCAGCTGAGAGCCCTG
CCCTCAGTGGAACCAGGAGTCTGATGGC
mm3_intron_Ece1_chr4_137922950 TCAGAGCATCCTGTTGCCAGTGTAGGCCCAGAACTGTCTGCCCAGCTCTTGGCCCAGGGATGGAGAGCAGACAGGCTGCT
CTGGGACTCCCCTTAAGAACCCTAATACGTGTCTCCAGGACTCAAAGAGTGCCC
mm4_intergenic_Gm15668|Kif22-ps_chr1_67389534 GGTTTTCAGCATGCACTTCCATTAAAATCTGTCTTTCTGCTCACCATCCCTGGTCCTAGGGGAATGTCTTTTATATGTCC
AGGC
mm4_intergenic_Gm6462|Gm25586_chr1_28959563 CCCTACCATGGGCAGAACCTATGGAGAATAGAATATTGTCCCTGGAATGGAGAGCTGGCAGGAGCTAAATCAGATAACAC
CATTTTTGCTTTCAGTAATTGAACTTTAATCCTATTTTGAGAGTCATTTCAGTAAGAGGT
mm4_intron_Kcnq1_chr7_143148909 GGCCACCTTGGATGATGACACCCAGGGATGGAGAGCTTAGTGGAAGGGAGACTTATCTTCCCAGTGCCCTTGGAATCAGA
GC
mm4_intergenic_Gm649|Gm14805_chrX_89337902 GGGGATAAATCACCCTCCATGGATCCTTGGATGAAGAACTGAAAGGAAACCCAATTTAACTATTTCTATGTAACAATGAT
CTTGAATACCATGGGACAGATTTGTGAGACCT
mm4_intron_Tnni1_chr1_135803713 CCTCAGAGAACTCAGCCTGCATGTAGTCCAGGGGTGGAGGGCTGAAAGTAAGTTGGTGTGCTGACTCAAGTGGAGAGGTG
ATGTCACTGATGGAGGGGACTCTTGGCCTGGATAGTCGGA
mm4_intron_Vmn2r19_chr6_123313854 GGAGCAGCTGTCTTCCTGAGAGCAACACCTGGATTGGAGAACTGAAAGGAGCCTAGGCTTGTCCTGTCAGCAGGACAACA
TGTCTTTCTATCACAGCCTCTGGC
mm4_intergenic_Atl3|Pla2g16_chr19_7549687 GCCCAGAAAGACTTGTTGGCATTCTTCCTGCTTTCCATCCCTGGTTGAGGATTGTGTCAGGTGAGGAAAAGGGCCTCACA
GTTTAGCAGGGGAGGG
mm4_intergenic_5033426O07Rik|Gm26815_chr8_121249014 CCCTCATGTCACACTTCCCCTGCATTGAGTCACCGTTCTGCCTGTCCCTAGCAGGCTGGCCCAAGGCCACCCGTGGGTGA
AGAGCCGAAAGGT
CAGCTGGGGTGTTCCCATTGCATCATGGGTCC
mm4_intergenic_Ccdc154|BC003965_chr17_25173861 GCAAGTTAGGGCTGGCAGTACCCACTAGATGAGCCTGGATGTGTGACCCACAAAAGGCTGCTTCAGCTCTTTCCTCTCTC
CATCCCTGG
ACCTCCTGAGCTCAGAGAGGACCTGGCTTAGAGGG
mm4_intergenic_Vimp|Chsy1/Gm26827_chr7_66092633 TTCCTTGGCATCGTCTTCCCGCAGACCCTGGGATGGAGGGACAAAAGGGATGAAGTGATGCGCAAGGGCAGTGGGCTCGC
CCAAATGGCCTGATTTGGAAGATGTGC
mm4_intron_Tns1_chr1_73926758 AGCCCGGTACAGAGACAGAATGAGGAAGAAGCCAGGGAATGAGAGAACCTGACTCCTACCACCTCTCCATCCCTGGCACA
GGTGGCCTCCACCTTGTGGGGAGGAAGGAAGGCGGACTGAACGTGTAG
mm4_intergenic_Gm13508|Gm25215_chr2_49348546 CCTGCGGAAGAAGATGGTGATGGGGGGGGGGGGGGCAGTTTTGCTGACCTGGGGTGGAGAGGAGAAAGGTAGAGTATATT
TGGGGACTGTGCAGGGCTATAGGGCCTGGGCATTC
mm4_intron_Cdkn1a_chr17_29095488 ATCGTGACGTGTTTTGTGGCCCGGGGTCCCCCTCCTTTCCTCTCCTCTCTCCACCCCAGGGTGACGCGCAGCTCCGGTGC
CCAAGCAGTTTTGGCGGGCGGGCAGCGCCGGGCAGGAAACTGACTCACCA
mm4_intron_Cntnap2_chr6_47137544 AGAACTTTGGGTGAGATGACTCAAATATTTTTATCAATCATTTCCTCTCCCCATCCCTGGAAAGTCTGTTCTGTTTGTAA
CTCACTGCATTAATTGCTAATGCCCTTCCTTCTGATGCTG
mm4_intron_Cntnap2_chr6_47119116 AGAGCTTTGGGTGAGATGACTCAAATATTTTTATCAATCATTTCCTCTCCCCATCCCTGGAAAGTCTGTTCTGTTTGTAA
CTCACTGCATTAACTGCTAACACCCTTCCTTCTGATGCTGGCCCAAA
mm4_intron_Rreb1_chr13_37833082 AAGACAGGATGGGGAGGGAACGAGGAGGGGGCCCAGGGATGGAGGGGAGAGAGGAAGTGCTTGTTTGTATGGGACCCCAG
C
mm4_intron_Mettl15_chr2_109205877 ACAAGCATTTCTTCTCTGTTCCTTCCATACAGCTCTCCATACCTGGAGTTAAGCATGATAACATGGATTTTTATTCTCAG
TAGCCAGTACTTGGCAAGTTGATAGAGAAATATAATCAGTCCCAGACATATCTAGCA
mm4_intron_Dusp10_chr1_184034806 AGTCTTTTGGCTCTGCTTGCCCTGGGATGAAGAGGGGAAATGAGTTTTGGTTTGGTTAAGATGCTTTATGGCAGTTTTCA
AAGCCCAAGGCTTTGCTCATGATGCTCAGTGGTGGCACGGAATA
mm4_intron_Pld5_chr1_175987635 TCATTTCCTCCTCCCTCCTTTATTCTCTTTGTCTCTCCATCCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATCTTCACCATTTCAGTCATGTCCA
mm3_intergenic_Gm16087|Gm16086_chr12_107027851 AGACAGAGGGACAGACGCTAGGCACCCCAAGTTCCCTGCTCCATTAGCCTCTCCATCCCTGGGACCACATTCTCCTGGCA
GCAGCCACAGTAATGGACAACTTTCCCCGGGGCGGGGTAACTTCAGAGAG
mm4_intergenic_Tmem189|Gm22355_chr2_167644223 GCCTGCAGCAGACACAGTTATGGGGGTAGTGAGGAGAGCCAGCCCTGGGGTGGAGAGCCCTCAGGAGACAGGCAGAAGAA
GGTGGAGCACACAGAAAGAGAGAGACCTCTTTCAAGTGCGGCTT
mm4_exon_Tulp2_chr7_45520993 GAAAGCACAGGGTCAGACCAACTGCAATGCAAACTATCTTGCCAGGAGACCAATGTCTTGGGATTCAGGGGGCCTCGGAA
AATGACTGTGATCCTTCCAGGAATGGACAGCCGGAAGCAGAGGATGAAAGTCCAGCCACA
mm4_intergenic_Asphd2|Sez6l_chr5_112392254 TCAGTCACCGTGAAAGGGTTTTCATGACGTCCAAACCTGGGATGGAGACCAGAGAGCAGCAACGTGAATGTTATAGACTA
GAAAAGGCCTAGCAGTTCCGGGAAGATGGAGATCCAGC
mm4_intron_Mad1l1_chr5_140044611 CCACATGGGGCTCTCAGAATTTCCTGGGATTGAGAGCCAGGAGGAGGAGGAGAGTAATGGGCACAGTGCCTGGAGTCGCA
GAGGTCAGTA
mm4_intron_Elfn1_chr5_139913296 TCCCCATAAACTTGGAGCGGTCCTTATGGCTGTCCATCCAGGGGTTGGGGTTAGGAGTCTGTGAAACCGATCTCCCTCCA
TTCCTAGCCTGCTTCTTCCTTAAGCTAGTGCCAGAGGGTGTCCCAGGCTGTGTTTA
mm4_intron_Golm1_chr13_59674221 TGAAAAGACCAAAGCCCTGCTCCCTTCCCCTCACCATCCCAGGACTTCCAGGGCTGCTCTGCAGGCCCAGGCTTCCTGCA
AGCTCCCTCCCTCTGTCCTCCAACT
mm4_intron_Tenm4_chr7_96468903 AAGGTGTTGGCCTTGGCAAAATAAGTAATATCATGGCCAGAGATGGAGAAGAGAAAGGAGAGTCAGCAGCTCTTTATGCA
GTTGCTATGAGCAAGGCTGTGCTGTTTTAGTGGATGCTTGAACTTAGGGGTGGGGAGAGG
mm4_intergenic_Gm13855|Gm14546_chr6_34253093 CAAGCCCTCAACGACAGCTAGCTGTTGGTGTTAGCGTAACACCTCTTAATTCTGTTTATATTACCTATCGCCTCTCCAAC
CCAGG
TAACAAAAGTTAGAAGAAAGCCATACCAACACTCAATGTG
mm4_intergenic_Gm22777|Spock1_chr13_57139054 GCCGTGAGTCACATCCAAGACCCTCTTTCCAGCCGTCTCCCTCCTTCATCTGTTTCTCCAGCCTTCCTTCTGGTCTCCAT
CCCAGG
ATCACAGCCTCCCAGTTCATCC
mm4_intron_Ksr2_chr5_117576372 GCTGTTTCTCAACTGGCCTTTATTGTGAAACACTTTGAGATGCATAAAATGTTATAGCACAGAGTTTCCTACGTTCCTTT
CAGCTAGTCATCCCCGG
CTCAGCACTGCCCAGGAAGGCTTCTACCTT
mm4_intron_Epha10_chr4_124914650 ATCCCGCCTAGAGTCCCTGGGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGTATGTTGGGTAATTG
GAAGCATCCTGTCTGCTCCCCATCACTGGAGCCTAGGTAATCAAGGCCGTGGAGGATG
mm3_intergenic_Rell1|Gm25608_chr5_63969227 TGGGTCCTGGGACTAGTCAGATGCGGGAAGAGGAAGGCAGAGGTTAGGCCAGGAAGCCCAGGATGGAGAGCAGACAGGAC
CAGCAGTTTCAGAATGGCG
mm4_intron_Ppfia2_chr10_106738410 GGATCCTGACAGCTGCCATTGGTGGGCAGGATGGAAAAGGGCCCGAGATGAAGAGAAGAAAGGATGGCACTGTTCTTAGT
ATGCATTCTTCCTTCTTCTACCCAGGTTACTGTCATCGAGTGTCACAGACCCA
mm4_intergenic_Gm25491|Eya1_chr1_14163370 GACTAAGATGCCTGCCCCTCCCCTGACCAGCTTTGCCTCTGGCACACCTGCTGTGTGGCTTCCTTCCACCTCTCAATCCC
TGG
GGCTTCCCTTGGACAAGCTCT
mm4_intergenic_Gm22845|Nt5c1b_chr12_10070541 ACACTTGGAAATGGCCTCCATCTCATTGCCTGGCATGGGGAGCTGGAAGGAACCCTCCACAGTCATAGCACA
mm4_intergenic_9030612E09Rik|Pdss2_chr10_43214980 GGGATACACACAGTATTACATAGCATTTTTAAGCAATGATAGATAAAATGAAAACCACCCCAGGGTGGAGAGGCAAAAGG
A
CTAGAATTGAGAATGATCTTGTGGGC
mm3_intergenic_Acox3|Htra3_chr5_35622138 GGGCCTCAGTGAACCATACATCAAGAAAGTCTTGTCATACCAGTCAGCTCCATCCTGTCACCTCTCCATCCCAAGTCATC
CAAGATTACACCAAAGCCTGAGCACA
mm4_intergenic_mmu-mir-6378|Gm25442_chr3_35066689 GGAAAGGAAAGAGGCCGCTTATTCATCAGTTGCTGCACCCCCCCCCAGCACCACCCCCCCAGCTACCCCACCAGAGATGG
AGAGAAGCAAGGA
ACACCTCCAGCATGTCTTTTACAGGACAACCCACCCA
mm3_intron_Dscam_chr16_96736513 GGCTGCAAGAAAAGCATCCCTGTTTCAGAGTCCCACTTGAAATCAGAGAATTGGTTGCTTTCTGCTATCCATCCCCAGTC
AGTGGCAAAGTCAGTGAGTCTCAAGCATGAAAGTGCTGACCCAC
mm4_intergenic_Rnf144b|4931429P17Rik_chr13_47636421 AGGACTCAAGAGAAACCCCAAAGAAACTGAAGATGAATATAAAAGACAGAACCAGGGATGGAGAGAGCATAGGACAAAAC
ACCAACTCTGCCTTCTTCTCAATCTTGCCTGGGGGGACCTGGACCTAATGAG
mm4_intergenic_Rora|F830001A07Rik_chr9_68775566 GTAGAAACGCTGGGCTCTCCTGTTAGCTTGATTAGCAGGACACTACTTGTATCTTCCCCTGGATGGAGCCCCGTAAGGAT
CAGTACATCCAGTTCCCAAACCCC
mm4_intron_Gse1_chr8_120304608 AGTGACCAAACCCTCTGCAGGGCTGAGCGATGTCCAAATTCCTGTTCCTTTTGGACCTCCATGCCAGGCCTTCTAGAAGG
GAGCCCTGTTTTGTGAATGGGCCG
mm4_intergenic_Rxfp3|Adamts12_chr15_11063185 GCAAAACACAGGAGCCAGTGTCCCTCAGAAAGTCTTGATAGCTATGTTTCCCAGTGTGGAGTCCTTTGGGCTTTCCATCT
AGGG
CCTTGCATTTTGGGTCCCTG
mm4_intron_R3hdm1_chr1_128131647 ACCTGGAACTTGGGGAGACTGAAGACCAACCTGAGCTAAACCGTGGACGGAGGGCAGAAAGGAGAGAGGGTTGATTTCAG
AAGACATTGTGGATAATGATAGACAGTGAGTTGGAACAGTGA
mm4_intergenic_Gm25736|Tmeff2_chr1_51029470 AGAGCTGGATATATGGCTCCATAGTAGAATGTTTGTTTCATGTGTGTAAATTTTTGGATTATATCCCTGGCATGGAGAGA
AGAAATGA
AAGAAAGAAAATAGGGGGCAGGGAGGAAAACTCACT
mm4_intron_Frmd4a_chr2_4414130 TGTTCTCACTGGTGCCATGGGACACCTGGCATGGACAGCTGAAGGGAATATCAGGAAGTGCAGTCTATCATGGCAGCC
mm3_intergenic_Chrm1/Gm22820|Slc3a2_chr19_8695728 TGTCCTTGCTCTGCTTCCTGGAACTACTCTGTTCAGCCTTGTCTCTACCCTGGGAGTGCATCCTCCAGTCCCAAGAATAA
AACTGACATCATTCTCTCCTCTTGTCTCTCCATCCCAGAGCCCAAGTCCCTATGTGGAA
mm4_intron_Ncapd2_chr6_125189713 GGCAGGGACTAGGAAAGACCTCCCTGGCCCAGATGGAGTGTCCCGGGGCTGTAGAACAGAAAGGATAACATCATTGCATG
GAGTCTCTCACCTCTAAGCTGGGATGAGAGGTGCTTGACG
mm4_intron_Atm_chr9_53476837 TCCATCCGAGGTGGCTAGTATCAAGTAGAACTCAGAAGACAGTGAGGGGATCTCTACTGCTGAACCAGAGCTGGAAAGCA
GAAAGGA
TGAGGGGCACAGAGGGAACACAACTGGCAGGATGTGCATTCA
mm3_intron_Ccl1_chr11_82254769 ACCTGACTGAGATTGGCAGCAGCTTGTCTCTGGAGTGACCATCTCAGGGATGGAGAGTGGAAGGGACAACCCAGAAGTGT
GGCCTGTGGAAC
mm4_intergenic_Pou3f1|Utp11l_chr4_124664679 CCCACATGGAAAGAGGGCTTGCCTCGGGTTTGTAGCAGGCATAAGGCCCCGTGTTATTTTTATTTCTTTTCTCTCGGCTC
TCTGTCCCTGG
AGGGTTCTGGGGAATAGCGGTGGCTCCGTTGCTCTCTCACACCCGAT
mm3_intergenic_Arhgef39|Gm12454_chr4_43503634 CCCTCAAGCGCCAGTTAAGAGTATGACCCCATCTGCTCCTGTGGCCTCTCCATCCCAGAGTAGACTAGCTATTCCCACAG
GCTGCCTTTGACACGCCCATCCAGTT
mm4_intergenic_Rnu1b6|5S_rRNA_chr3_96460453 CCACCCAGGAGTGACATCACGCCCCTTCCTTTCGCCGCCCGATCCCGGGGCCTGTTGGACTTTCTCACTGGCGCAGTGCT
GGAGCGGTGAAGGACCAGGGACAAGTCACCTTGCACCGTCTCTTACCCCTA
mm4_intergenic_Fcgr1|Gm24830_chr3_96328137 TGCGCAAGTGAGAAAGTCCAACAGGCCCCGGGATCGGGCGGCGAAAGGAAGGGGCGTGATGTCACTCCTGGGTGG
mm4_intergenic_BC107364|Gm26232_chr3_96450158 TAGGGGTAAGAGACGGTGCAAGGTGACTTGTCCCTGGTCCTTCACCGCTCCAGCACTGCGCCAGTGAGAAAGTCCAACAG
GCCCCGGGATCGGGCGGCGAAAGGAAGGGGCGTGATGTCACTCCTGGGTGG
mm4_intron_Dapk1_chr13_60695169 TGTCTCAGTACCTCCGAGGGATTGCCATGTAATTGCATGGCCTCATTGTTATCTAACCACTCGCTGTCTTGCTAACTTCC
TTTCTGATCTCCATGCGTGG
GAATGAGATCTGGTTGTGTGTTC
mm4_intergenic_Tex16|Gm6335_chrX_112162509 TATACCGGGAGCAGCATGTGGCGGAGCTGCGCTGTGCTCCACTCCTTTCTGCTCTCCGGCGCTGGCCCGCTCACCGGCTG
TAAAAAATGATGAAAGAAACCACTTACTACGATGTTTTGGGAGTCAAATCCAATGCCA
mm4_exon_Dnaja1_chr4_40722599 AACTTTCCAGAACGCTCGGTGGGTGGTGGAGGAGCAGCGTGTGGCTGAGCTGCGCTGTGCTCCGCTCCTTTCCGCTCTCC
GGCGCCGG
CCCGCTCACCGGTGAGTCTCCGGCCTCCCTC
mm4_intron_Tbl1xr1_chr3_22144884 GGCCAAGGTAGAGGAGAAGCTGATGGAGAACCAGTCCTAAAGTGCTATAGTGCACAAGTGCTGGAACCTGGGCTGCAGAG
CAGACAGGA
CTGTGTCTCTAGAAAGCAAATGTAAAGTAAAGGGCAGATGATGTGCAGG
mm4_intron_Gm14040_chr2_129515346 AATTCTGTCTCGGCAAAGCCCAGCGAAGCGATTTATCTCCTTTCCCGGGAGGGAGGGCAGAAGGGAGAGAAGGGAGCCCA
GGAAGCCAGAACATTTCCCTTTCTCAGGGCCAGACTTGGTTTACTCAGCCCCAGC
mm4_intron_Gm12160_chr11_45175291 ACCACTGAACATCTGCTGGGAATCAGAGTTCTACACCCAGGGAAGGAGAACCGTACGGAGGGCGTGGCTTCCCTTAAAGT
G
mm4_intron_Filip1_chr9_79828428 TTTGCCACCCTGATGCATCCATCCCCTTGGGCTCTCCTTCCCAGGTGTCACTTTCACCTCGGTCCCTC
mm4_intron_Cntn4_chr6_106193413 CCATGCCCTGTTTCTCCCTTGAGATGCCTCTCGGCTCTCATTCCCAGGGAAGAATTCCATATGGCACCATAGAGAGGTCA
C
mm4_intron_Batf2_chr19_6161622 GACTGGGAGCTTGGACGAAAGAGCGATTGTGTTCTCCTTCCAGCTCTGCCTCCCTGGGGTGGGAGACCTTAACTCTCTGG
GTTTTATGACCCCACCCTTTAGGAATG
mm4_intron_Atp6v0a2_chr5_124711714 CCAGGGATCACAGCTCCATCCCGCTCTCCTTCCCAGGGGTCACAGCTCCGTCCCGCTCTCCTTCCCAGGGATCACAGCTC
GTCCCGCTCTCCTTCCCAGGGGTCACAGTTCCATCC
mm4_intron_Atp6v0a2_chr5_124711682 TCTCCTTCCCAGGGATCACAGCTCCATCCCGCTCTCCTTCCCAGGGGTCACAGCTCCGTCCCGCTCTCCTTCCCAGGGAT
CACAGCTCGTCCCGCTCTCCTTCCCAGGGGTCACAGTTCCATC
mm4_intron_Atp6v0a2_chr5_124711618 TCTCTTTCCCAGGGGTCACAGCTCCGTCCCGCTCTCCTTCCCAGGGGTCACAGCTCCATCCCGCTCTCCTTCCCAGGGGT
CACAGTTCCGTCCCGCTCTCCTTCCCAGGGATCACAGCTCCATC
mm4_intron_Atp6v0a2_chr5_124711586 TCTCTTTCCCAGGGGTCACAGCTCCGTCCCGCTCTCCTTCCCAGGGGTCACAGCTCCATCCCGCTCTCCTTCCCAGGGGT
CACAGTTC
mm4_intron_Atp6v0a2_chr5_124711490 CAGGGGTCACAGTTCCATCCCGCTCTCCTTCCCAGGGGTCACAGCTCCGTCCCGCTCTCCTTCCCAGGGGTCACAGCTCC
GTCCCGCTCTCCTTCCCAGGGGTCACAGCTCCGTCCCGCTCTCTTTCCCAGGGGTCACA
mm4_intron_Atp6v0a2_chr5_124711458 CATCCTCACTTCCCAGCTCCGTCCCTGCTCTCCTTCCCAGGGGTCACAGTTCCATCCCGCTCTCCTTCCCAGGGGTCACA
GCTCCGTCCCGCTCTCCTTCCCAGGGGTCA
mm4_intron_Amfr_chr8_93979122 GGAAAGAAGGAGCCCGAACATATAAGCAATACCCAGGATTAACTCCCAAGAGCTCCACCNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNN
TGAAAAAGAAAAGAATTTCCTTTGACAAACCCTTCCCAAGTTCCC
mm4_intergenic_Tada3|Arpc4_chr6_113378056 ACTCGACCGCTACTGTCTCATAGCAGGTCTTCTACAAGTCCCAGAGGTCCCAGTGTGAAATCGCCCCGGAGATCTGAATA
TCTCTCGGCTCTCCCTCCCCGG
TCCTACCCGAGATGGTCCAG
mm4_intergenic_Rps6-ps2|mmu-mir-8110_chr8_88925683 CACCCAGCCACTTGTCTCTTCCTTGGGCCTGTTGTGGCAGCTTGGGCCAGGTTGGCTGTTGTATTCAGCACCATGGCTGC
AGAGACGAAAGGA
TAACTTCCTGGGTGATGTGCT
mm4_intergenic_Loxl4|Gm25216_chr19_42692212 TAGAGGCATGCTCTTGCTGGTCTCTCTCTCTCTAACCCTCCTTCCTTTCCTTTCTCCTTCCCGGGTCTCCCCACTGGTTT
GTTCACAGGGACCC
mm4_intergenic_Kirrel3|Gm23702_chr9_34947581 AGGCAAGAACTGACTCTGAGCAGAGCTTTAGCTTCCCTGGGAGGCAGAGGAGAAAGGAGCCAGAGAATGAGGGGAAAGTG
GGGGAGTGAACTGTACCTAAGAAGCTGAGCACCCC
mm4_intergenic_Itgb4|B230344G16Rik/Itgb4_chr11_115987545 CCCCTGTGTGGCTTTGTCTACGGTTTCTTCTCTTCAGCTCTCCTTCCCAGGGGTGGTGGGGAATGAAATAGTAAGCTAGG
TGTATCTGGAGAAGGGCAGACAGTTAATAGAGATGGAGGTGTGAGCGAG
mm4_intergenic_Gm26878|Fendrr_chr8_120927558 TCCCGTATTCCTCTCTTCCCATCTTTCCTCCTCCCTCCGGCTCCCCCTCCCTGGCCAGGATAAAGAAACATAGTTTCTTT
TAGCTAACAAAGGATATAGAACAGAAAGCAGACTCTTCACTGCTAGGGTGGTGG
mm4_intergenic_Gm26228|Gdf10_chr14_33789539 AACCCTGACCTACAGCCTCTGCCTCTCCTTTCCTCTCTGCCTCCCAGGGCTCCACATTGCCATCACAATTCTCCCTGGCC
AGGGTCTTCGCAATGCTGTTG
mm4_intergenic_Gm22914|Skap2_chr6_51782698 CGGGGTCACTGGTTCTCTTCTGTCCTGGGAAGGAGAGCCGAAGATAAAAGGAGCCCCTTTGAGGAAGAC
mm4_intergenic_Gm15756/Aldh1l1|Aldh1l1_chr6_90597133 AGGAGGAGACTGAGTCAGCAGAGAACCTGTGCACCATGCCTGGGCTGCCTGCCTCTTAAAACACAAAAGAGGTTCTGGCT
TCCCTTTGCCTCTCCTTCCCTGGGCCAGAATGGCCTCCAGGCCCAGATGAGCAAAA
mm4_intergenic_Gm14318|B230312C02Rik_chr2_180352653 GCATCCTCACAAGGTGGGAAGAGACCAAAGGTCTGGTTCACCCTTCTGGCTCTCCCTCCCTGGACAGCTGTTTGCCAGTA
CCACATCCTCCT
mm4_intergenic_Gm12450|Spag17_chr3_99806993 GGAAGTCTTCATTCCGCCCAGCATAGATCTATCTGGCAAACAGTCACCAAGGAAGGAGAGCAGAATGGAAATAAGTAGGA
AAACAGTCTTCTACGTTGA
mm4_intergenic_Gm10398|Gm26660_chr14_24978689 GTGTGACTCTGTGCGTCCTTTGCCACTTTCTGCTCTGCATCCCTGGACACTCAGTGGGAAGAGCTCTGCACACCCTT
mm4_intergenic_Fbxo2|Ptchd2_chr4_148173939 AGTATCGGGCTGCGTTTGAAATCAGACCATCCCGGGGAGGGAGCGCCGGAGGGAGGGAGCGCCAGCCCCCGGACCAATTA
CTCTGCTCGCCGA
mm4_intergenic_Efcab4b|Prmt8_chr6_127661089 ATATGCTTGAGCTTGGCCGAGCCATAGTCCATGATGGCTGGACTGTGAGACTCAGGAAGTCAAAGGCATCCGTCCTTCCT
GCCGCCTCTGCATCCCCGG
CCCACTTGCTAGCTTACCACCCCTGGGAGAATGGACCTA
mm4_intergenic_Agbl3|Gm13864_chr6_34785928 TGTGTCAGAGCACACGTGAACTACAATTGCACTCATTTCTGCTCTCCCACCCTGGTCCCCAGTCTCTTTTCTTCAATCCC
TTTGTCTCTGCCT
mm4_exon_Frmd4a_chr2_4401119 CGCTAGGGAGGCAGAAGATCTCATTGTTGTCCTGGGATCCAGTGGCGAAAGGAGGCCCCTCCAGCGGGCCCGCGCTCAAT
CCCTGCTTCGCAAGCGATCGAGC
mm4_exon_Dnm3_chr1_162134565 GCTGGACATGAAGCCCTTCTCCACATCCCGGACCTTCAGGTTGTCCAGGGGAAGCATATATTTCTTTTCTTTCTCCTGGG
ATACAGAGGTGAAAGGA
GGGGATGTTAATAGAACATCACACCAATGGCAACCAACACACC
mm3_intron_Cib4_chr5_30540205 CCGTTGCCTTCCTTGAGCTTTTCCTCATTGCCATCCTTGTCACTAACATTTCCATCCATTTGCTAGGGTCCTTTCGTCTC
TGCATCCTGGG
TCTTTGAGTTTGATTACTAATTTTAGCTGTCTCAGATATGTGTATACCC
mm3_intergenic_4930564D02Rik|1700095B22Rik_chr3_105268394 TCCATTTTCGCTCCAGTTGTTTAAAGACATTCCACGAGCATCCTTGTGGCTCTGCATCCCAGGGGATATTCCAGGGAGAG
ACTGCTTTATGACTCAAAGAGAGAAGGGTAGAGTAAATGGAGGTGCATGCCTTCTAC
mm3_exon_A930016O22Rik/Ckm_chr7_19419065 ACTGAAGATTGCTGAACTCTCAGAACCCTGGGATGCAAATCCGAAAGGAGAATCTTGGGATCCAAGGGGATATAACATTG
GGTGTAGGACCTGTGGTCACAGGAGGTATACAACGCCCCT

Input file for Crispresso

Crispresso, written by Luca Pinello, is a software package to quantify the Cas9-induced mutations on off- or on-targets.

Click here to download an amplicon input file for Crispresso. For each off-target, it includes the off-target name, its PCR amplicon and the guide sequence. Keep a copy of this file.

After sequencing, run CRISPRessoPooled. The tool will map the reads to the amplicons and analyse the mutations:
CRISPRessoPooled -r1 Reads1.fastq.gz -r2 Reads2.fastq.gz -f crisporAmplicons_mN1FkRligAThyqKv3eg3.txt --name MY_EXPERIMENT

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CRISPR/Cas9 Guide Designer for chordate vertebrate ecdysozoans lophotrochozoans protostomes spongi corals plants butterflies metazoans genomes fruitflies insects nematodes mammals.