CRISPOR

DNA break repair outcome predictions

Guide: GACTTCCCGGGAGCGTGGCG GGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

mh- Score	Sequence	Effect
	`AGCCCGCGAGCCGGCCCTCCGACTTCCCGGGAGCGTGGCGGGGGAGGCCAGGAGGAGGAGCCGGTCGATGGACTAGCAGG`	(Wild-type sequence)
469	`CCGGCCCTCCGACTTCCCGGGAG-------------GCCAGGAGGAGGAGCCGGTCGATG`	`13 bp deleted → frameshift`
382	`CCGGCCCTCCGACTTCCCGGGAGCGTGGC---------CAGGAGGAGGAGCCGGTCGATG`	`9 bp deleted → no frameshift`
329	`CCGGCCCTCCGACTTCCCGGG------------GAGGCCAGGAGGAGGAGCCGGTCGATG`	`12 bp deleted → no frameshift`
327	`CCGGCCCTCCGACTTCCCGGGAGCGTGG----GGAGGCCAGGAGGAGGAGCCGGTCGATG`	`4 bp deleted → frameshift`
311	`CCGGCCCTCCGACTTCCCGGGAGCGTGG-----GAGGCCAGGAGGAGGAGCCGGTCGATG`	`5 bp deleted → frameshift`
296	`CCGGCCCTCCGACTTCCCGGGAGCGTGG------AGGCCAGGAGGAGGAGCCGGTCGATG`	`6 bp deleted → no frameshift`
245	`CCGGCCCTCCGACTTCCCGGGAG---------------------GAGGAGCCGGTCGATG`	`21 bp deleted → no frameshift`
233	`CCGGCCCTCCGACTTCCCGGGAGC---------------------------CGGTCGATG`	`27 bp deleted → no frameshift`
230	`CCGGCCCTCCGACTTCCCGGG-----------GGAGGCCAGGAGGAGGAGCCGGTCGATG`	`11 bp deleted → frameshift`
210	`CCGGCCCTCCGACTTCCCGGGAG------------------------GAGCCGGTCGATG`	`24 bp deleted → no frameshift`
198	`CCGGCCCTCCGACTTCCCGGGAGCGTGG--------------AGGAGGAGCCGGTCGATG`	`14 bp deleted → frameshift`
198	`CCGGCCCTCCGACTTCCCGGGAGC--------------CAGGAGGAGGAGCCGGTCGATG`	`14 bp deleted → frameshift`
198	`CCGGCCCTCCGACTTCCCGG--------------AGGCCAGGAGGAGGAGCCGGTCGATG`	`14 bp deleted → frameshift`
179	`CCGGCCCTCCGACTTCCCGGG----------------CCAGGAGGAGGAGCCGGTCGATG`	`16 bp deleted → frameshift`
170	`CCGGCCCTCCGACTTCCCGGGAGCGTGG-----------------AGGAGCCGGTCGATG`	`17 bp deleted → frameshift`
170	`CCGGCCCTCCGACTTCCCGG-----------------CCAGGAGGAGGAGCCGGTCGATG`	`17 bp deleted → frameshift`
153	`CCGGCC---------------------------------AGGAGGAGGAGCCGGTCGATG`	`33 bp deleted → no frameshift`
147	`CCGGCCCTCCGACTTCCCGGGAGCGTGG--------------------AGCCGGTCGATG`	`20 bp deleted → frameshift`
146	`CCGGCCCTCCGACTTCCCGG----------------------------------TCGATG`	`34 bp deleted → frameshift`
140	`CCGGCCCTCCGACTTCCC---------------------AGGAGGAGGAGCCGGTCGATG`	`21 bp deleted → no frameshift`
133	`CCGGCCCTCCGACTTCCCGGGAGCGTGGC----------------------CGGTCGATG`	`22 bp deleted → frameshift`
133	`CCGGCCCTCCGACTTCCCGG----------------------AGGAGGAGCCGGTCGATG`	`22 bp deleted → frameshift`
133	`CCGGCCCTCCGACTTCC----------------------AGGAGGAGGAGCCGGTCGATG`	`22 bp deleted → frameshift`
126	`CCGGCCCTCCGACTTCCCGGGAGCGTGGCG-----------------------GTCGATG`	`23 bp deleted → frameshift`
122	`CCGGCCCTCCGACTTCCCGGGAG------------------GAGGAGGAGCCGGTCGATG`	`18 bp deleted → no frameshift`
114	`CCGGCCCTCCGACTTCCCGG-------------------------AGGAGCCGGTCGATG`	`25 bp deleted → frameshift`
109	`CCGGCCCTCCGACTTCCCGGGAGCGTGG--------------------------TCGATG`	`26 bp deleted → frameshift`
103	`CCGGCCCTCCGACTTCCCGGGAGCGTGGCG---------------------------ATG`	`27 bp deleted → no frameshift`
98	`CCGGCCCTCCGACTTCCCGGGAGCG----------------------------GTCGATG`	`28 bp deleted → frameshift`
98	`CCGGCCCTCCGACTTCCCGG----------------------------AGCCGGTCGATG`	`28 bp deleted → frameshift`
98	`CCGG----------------------------GGAGGCCAGGAGGAGGAGCCGGTCGATG`	`28 bp deleted → frameshift`
95	`CCGGCCCTCCGA-----------------------GGCCAGGAGGAGGAGCCGGTCGATG`	`23 bp deleted → frameshift`
94	`CCGGCCCTCC-----------------------------AGGAGGAGGAGCCGGTCGATG`	`29 bp deleted → frameshift`
94	`CCGG-----------------------------GAGGCCAGGAGGAGGAGCCGGTCGATG`	`29 bp deleted → frameshift`
89	`CCGG------------------------------AGGCCAGGAGGAGGAGCCGGTCGATG`	`30 bp deleted → no frameshift`
80	`CCGGCCCTCCGACTTCCCGGGAGCG--------------------------------ATG`	`32 bp deleted → frameshift`
80	`CCGGCCC--------------------------------AGGAGGAGGAGCCGGTCGATG`	`32 bp deleted → frameshift`
76	`CCGGCCCTCCGACTTCCCGGG---------------------------------TCGATG`	`33 bp deleted → no frameshift`
73	`CCGGCCCTCCG------------------------------------------GTCGATG`	`42 bp deleted → no frameshift`
70	`CCGGCCCTCCGACTTCCCGGGAGCGT-----------------------------CGATG`	`29 bp deleted → frameshift`
69	`CCGGCCCTCCGACTTCC-----------------------------------GGTCGATG`	`35 bp deleted → frameshift`
65	`CCGG--------------------------------------------------TCGATG`	`50 bp deleted → frameshift`
63	`CCGGCCCTCCGA-------------------------------GGAGGAGCCGGTCGATG`	`31 bp deleted → frameshift`
62	`CC-------------------------------------AGGAGGAGGAGCCGGTCGATG`	`37 bp deleted → frameshift`
60	`CCGGCCCTCCGACTTCCCG--------------------------------------ATG`	`38 bp deleted → frameshift`
60	`CCGGCC----------------------------------------------GGTCGATG`	`46 bp deleted → frameshift`
60	`CCGG--------------------------------------AGGAGGAGCCGGTCGATG`	`38 bp deleted → frameshift`
57	`CCGGCCCTCCGACTTCCCGGGAGCGTG---------------------------------`	`33 bp deleted → no frameshift`
54	`CCGGCCCTCCGA----------------------------------GGAGCCGGTCGATG`	`34 bp deleted → frameshift`
51	`CCGG-----------------------------------------AGGAGCCGGTCGATG`	`41 bp deleted → frameshift`
50	`CCGGCCCTCCGA----------------------------------------------TG`	`46 bp deleted → frameshift`
49	`CCGGCCCTCCGACTTCCCGGGA------------------------------------TG`	`36 bp deleted → no frameshift`
47	`CCGGCCCTCCGA-------------------------------------GCCGGTCGATG`	`37 bp deleted → frameshift`
44	`CCGG--------------------------------------------AGCCGGTCGATG`	`44 bp deleted → frameshift`
42	`CCGGCCC---------------------------------------------GGTCGATG`	`45 bp deleted → no frameshift`
40	`CCGGCCCTCCGACTTC----------------------------------------GATG`	`40 bp deleted → frameshift`
28	`CCGGCCCTC-----------------------------------------------GATG`	`47 bp deleted → frameshift`
26	`CCG------------------------------------------------------ATG`	`54 bp deleted → no frameshift`

Version 5.01 - Documentation - Contact us - Downloads/local installation - Citation - License