CRISPOR

DNA break repair outcome predictions

Guide: GATGGAGAGCCGAAAGGACA TGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

mh- Score	Sequence	Effect
	`CCCCCTCTGGAGAGTCCGGGGATGGAGAGCCGAAAGGACATGGTTATGTTTCTGGATGGGGGTCAGCTTGGCACTCTGGT`	(Wild-type sequence)
296	`AGAGTCCGGGGATGGAGAGCCGAAAGG------TTATGTTTCTGGATGGGGGTCAGCTTG`	`6 bp deleted → no frameshift`
203	`AGAGTCCGGGGATGGAGAGCCGAAAGGA------------------TGGGGGTCAGCTTG`	`18 bp deleted → no frameshift`
203	`AGAGTCCGGGGATGG------------------TTATGTTTCTGGATGGGGGTCAGCTTG`	`18 bp deleted → no frameshift`
183	`AGAGTCCGGGGATGG----------------------------------GGGTCAGCTTG`	`34 bp deleted → frameshift`
133	`AGAGTCCGGGGATGGA------------------------------TGGGGGTCAGCTTG`	`30 bp deleted → no frameshift`
133	`AGAGTCCGGGGATGGAGAGCCGAAAGG----------------------GGGTCAGCTTG`	`22 bp deleted → frameshift`
133	`AGAGTCCGGGG----------------------TTATGTTTCTGGATGGGGGTCAGCTTG`	`22 bp deleted → frameshift`
126	`AGAGTCCGGGGATGGAGAGCCGAAAGG-----------------------GGTCAGCTTG`	`23 bp deleted → frameshift`
126	`AGAGTCCGGG-----------------------TTATGTTTCTGGATGGGGGTCAGCTTG`	`23 bp deleted → frameshift`
120	`AGAGTCCGGGGATGGAGAGCCGAAAGG------------------------GTCAGCTTG`	`24 bp deleted → no frameshift`
120	`AGAGTCCGGGGATG------------------------TTTCTGGATGGGGGTCAGCTTG`	`24 bp deleted → no frameshift`
120	`AGAGTCCGG------------------------TTATGTTTCTGGATGGGGGTCAGCTTG`	`24 bp deleted → no frameshift`
114	`AGAGTCCGGGGATGGAGAGCCGAAAGG-------------------------TCAGCTTG`	`25 bp deleted → frameshift`
108	`AGAGTCCGGGG----------------------------------------GTCAGCTTG`	`40 bp deleted → frameshift`
103	`AGAGTCCGGGG-----------------------------------------TCAGCTTG`	`41 bp deleted → frameshift`
95	`AGAGTCCGGGGATGGAGAGCCGA-----------------------TGGGGGTCAGCTTG`	`23 bp deleted → frameshift`
86	`AGAGTCCGGGGATGGAGAGCCGAAAGGACA-------------------------GCTTG`	`25 bp deleted → frameshift`
85	`AGAGTCCGGGG---------------------------------------GGTCAGCTTG`	`39 bp deleted → no frameshift`
78	`AGAGTCCGGGGATGGAGAGC-------------------------------------TTG`	`37 bp deleted → frameshift`
74	`AGAGTCCGGGGATGGAGA----------------------------TGGGGGTCAGCTTG`	`28 bp deleted → frameshift`
73	`AGAGTCCGGG------------------------------------------TCAGCTTG`	`42 bp deleted → no frameshift`
70	`AGAGT-----------------------------TATGTTTCTGGATGGGGGTCAGCTTG`	`29 bp deleted → frameshift`
69	`AGAGTCCGGGGATGG-----------------------------------GGTCAGCTTG`	`35 bp deleted → frameshift`
69	`AGAGTCCGGG-----------------------------------ATGGGGGTCAGCTTG`	`35 bp deleted → frameshift`
66	`AGAGTCCGGGGATGGAGAGCCGAAAG------------------------------CTTG`	`30 bp deleted → no frameshift`
66	`AGAGTCCGGGGATGG------------------------------------GTCAGCTTG`	`36 bp deleted → no frameshift`
66	`AGAGTCCGG------------------------------------ATGGGGGTCAGCTTG`	`36 bp deleted → no frameshift`
62	`AGAGTCCGGGGATGG-------------------------------------TCAGCTTG`	`37 bp deleted → frameshift`
60	`AGAGTCCGGGG--------------------------------------GGGTCAGCTTG`	`38 bp deleted → frameshift`
54	`AGAGT----------------------------------TTCTGGATGGGGGTCAGCTTG`	`34 bp deleted → frameshift`
49	`AGAGTC------------------------------------TGGATGGGGGTCAGCTTG`	`36 bp deleted → no frameshift`
46	`AGAGTCCGG-------------------------------------------TCAGCTTG`	`43 bp deleted → frameshift`
45	`AGAGTC------------------------------------------------AGCTTG`	`48 bp deleted → no frameshift`
42	`AGAGTCCGGGGATGGAG---------------------------------------CTTG`	`39 bp deleted → no frameshift`
34	`AGA-------------------------------------------TGGGGGTCAGCTTG`	`43 bp deleted → frameshift`
30	`AGAGTCCGGGGATG----------------------------------------------`	`46 bp deleted → frameshift`
22	`AGAG----------------------------------------------------CTTG`	`52 bp deleted → frameshift`
20	`AG------------------------------------------------------CTTG`	`54 bp deleted → no frameshift`

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