CRISPOR

DNA break repair outcome predictions

Guide: GAAGAGGAGCTAAGGCGACA AGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

mh- Score	Sequence	Effect
	`CTCCCTGGGATCTTGGCTGAGAAGAGGAGCTAAGGCGACAAGGCAGGCGCCGGGTAGCCCCAGCGCCGTGTCGACGGCGA`	(Wild-type sequence)
494	`TCTTGGCTGAGAAGAGGAGCTAAGGCG------------CCGGGTAGCCCCAGCGCCGTG`	`12 bp deleted → no frameshift`
469	`TCTTGGCTGAGAAGAGGAGCTAAGGC--------AGGCGCCGGGTAGCCCCAGCGCCGTG`	`8 bp deleted → frameshift`
233	`TCTTGGCTGAGAAGAGGAGCTAAGGCGACA-----GGCGCCGGGTAGCCCCAGCGCCGTG`	`5 bp deleted → frameshift`
224	`TCTTGGCTGAGAAGAGG----------------CAGGCGCCGGGTAGCCCCAGCGCCGTG`	`16 bp deleted → frameshift`
198	`TCTTGGCTGAGAAGAGGAGCTAAGGC--------------CGGGTAGCCCCAGCGCCGTG`	`14 bp deleted → frameshift`
198	`TCTTGGCTGAGAAGAGGAGC--------------AGGCGCCGGGTAGCCCCAGCGCCGTG`	`14 bp deleted → frameshift`
188	`TCTTGGCTGAGAAGAGGAGCTAAGGCG---------------GGTAGCCCCAGCGCCGTG`	`15 bp deleted → no frameshift`
184	`TCTTGGCTGAGAAGAGG--------------------CGCCGGGTAGCCCCAGCGCCGTG`	`20 bp deleted → frameshift`
162	`TCTTGGCTGAGAAGAGGAGCTAAGG------------------GTAGCCCCAGCGCCGTG`	`18 bp deleted → no frameshift`
162	`TCTTGGCTGAGAAGAGGAGC------------------GCCGGGTAGCCCCAGCGCCGTG`	`18 bp deleted → no frameshift`
156	`TCTTGGCTGAGAAGAGGAG-------------GCAGGCGCCGGGTAGCCCCAGCGCCGTG`	`13 bp deleted → frameshift`
155	`TCTTGGC---------------------------AGGCGCCGGGTAGCCCCAGCGCCGTG`	`27 bp deleted → no frameshift`
154	`TCTTGGCTGAGAAGAGGAGCTAAGG-------------------TAGCCCCAGCGCCGTG`	`19 bp deleted → frameshift`
148	`TCTTGGCTGAGAAGAGGAGCTAAGGCG----------------------------CCGTG`	`28 bp deleted → frameshift`
147	`TCTTGGCTGAGAAGAGGAGC--------------------CGGGTAGCCCCAGCGCCGTG`	`20 bp deleted → frameshift`
133	`TCTTGGCTGAGAAGAGGAGCTAAGGC----------------------CCCAGCGCCGTG`	`22 bp deleted → frameshift`
128	`TCTTGGCTGAGAAGAGGAG-----------------GCGCCGGGTAGCCCCAGCGCCGTG`	`17 bp deleted → frameshift`
127	`TCTTGGC-------------------------------GCCGGGTAGCCCCAGCGCCGTG`	`31 bp deleted → frameshift`
123	`TCTTGGCTGAGAAGAGGAGC----------------------------CCCAGCGCCGTG`	`28 bp deleted → frameshift`
122	`TCTTGGCTGAGAAG------------------GCAGGCGCCGGGTAGCCCCAGCGCCGTG`	`18 bp deleted → no frameshift`
109	`TCTTGGCTGAGAAGAGG--------------------------GTAGCCCCAGCGCCGTG`	`26 bp deleted → frameshift`
105	`TCTTGGCTGAG---------------------GCAGGCGCCGGGTAGCCCCAGCGCCGTG`	`21 bp deleted → no frameshift`
103	`TCTTGGCTGAGAAGAGG---------------------------TAGCCCCAGCGCCGTG`	`27 bp deleted → no frameshift`
99	`TCTTGGCTGAGAAGAGGAGCTAAGGCGACA----------------------GCGCCGTG`	`22 bp deleted → frameshift`
99	`TCTTGGCTGAGAAG----------------------GCGCCGGGTAGCCCCAGCGCCGTG`	`22 bp deleted → frameshift`
95	`TCTTGGCTGAGAAGAGGAGCTAAG-----------------------CCCCAGCGCCGTG`	`23 bp deleted → frameshift`
91	`TCTTGGCTGAGAAGAGGAGC----------------------------------GCCGTG`	`34 bp deleted → frameshift`
89	`TCTTGGCTGAGAAGAGGAGCTAAGGC------------------------------CGTG`	`30 bp deleted → no frameshift`
86	`TCTTGGCTGAG-------------------------GCGCCGGGTAGCCCCAGCGCCGTG`	`25 bp deleted → frameshift`
84	`TCTTGGCTGAGAAGAGGAGCTAAGGCG-------------------------------TG`	`31 bp deleted → frameshift`
76	`TCTTGGC---------------------------------CGGGTAGCCCCAGCGCCGTG`	`33 bp deleted → no frameshift`
70	`TCTTGGCTGAGAAGAGGAGCTAAG-----------------------------CGCCGTG`	`29 bp deleted → frameshift`
66	`TCTTGGCTGAGAAGAGGAGC------------------------------------CGTG`	`36 bp deleted → no frameshift`
63	`TCTTGGCTGAGAAGAG-------------------------------CCCCAGCGCCGTG`	`31 bp deleted → frameshift`
62	`TCTTGG-------------------------------------GTAGCCCCAGCGCCGTG`	`37 bp deleted → frameshift`
60	`TCTTGGCTGAGAAGAGGAGCTA------------------------GCCCCAGCGCCGTG`	`24 bp deleted → no frameshift`
60	`TCTTGG--------------------------------------TAGCCCCAGCGCCGTG`	`38 bp deleted → frameshift`
57	`TCTTGGCTGAGAAG---------------------------------CCCCAGCGCCGTG`	`33 bp deleted → no frameshift`
51	`TCTTGGC-----------------------------------------CCCAGCGCCGTG`	`41 bp deleted → frameshift`
49	`TCTTGGCTGAG------------------------------------CCCCAGCGCCGTG`	`36 bp deleted → no frameshift`
47	`TCTTGGCTGAGAAGAG-------------------------------------CGCCGTG`	`37 bp deleted → frameshift`
42	`TCTTGGCTGAGAAG---------------------------------------CGCCGTG`	`39 bp deleted → no frameshift`
38	`TCTTGGC-----------------------------------------------GCCGTG`	`47 bp deleted → frameshift`
36	`TCTTGGCTGAG------------------------------------------CGCCGTG`	`42 bp deleted → no frameshift`
34	`TCTTGGC-------------------------------------------------CGTG`	`49 bp deleted → frameshift`
23	`TCTTGGCTG---------------------------------------------------`	`51 bp deleted → no frameshift`
19	`TCTTG-------------------------------------------------------`	`55 bp deleted → frameshift`

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