CRISPOR

DNA break repair outcome predictions

Guide: CATGGTTATGTTTCTGGATG GGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

mh- Score	Sequence	Effect
	`GGGATGGAGAGCCGAAAGGACATGGTTATGTTTCTGGATGGGGGTCAGCTTGGCACTCTGGTTGGTAAGAGGGTCTCTAA`	(Wild-type sequence)
311	`GCCGAAAGGACATGGTTATGTTTCTGG-----GGTCAGCTTGGCACTCTGGTTGGTAAGA`	`5 bp deleted → frameshift`
296	`GCCGAAAGGACATGGTTATGTTTCTGG------GTCAGCTTGGCACTCTGGTTGGTAAGA`	`6 bp deleted → no frameshift`
282	`GCCGAAAGGACATGGTTATGTTTCTGG-------TCAGCTTGGCACTCTGGTTGGTAAGA`	`7 bp deleted → frameshift`
240	`GCCGAAAGGACATGGTTATGTTTCTGG------------------------TTGGTAAGA`	`24 bp deleted → no frameshift`
224	`GCCGAAAGGACATGGTTATGTTTCTGG----------------CACTCTGGTTGGTAAGA`	`16 bp deleted → frameshift`
193	`GCCGAAAGGACATGGT-------------------CAGCTTGGCACTCTGGTTGGTAAGA`	`19 bp deleted → frameshift`
170	`GCCGAAAGGACATGG-----------------GGTCAGCTTGGCACTCTGGTTGGTAAGA`	`17 bp deleted → frameshift`
164	`GCCGAAAGGACATGGTTATGTTTCTGGATG------------GCACTCTGGTTGGTAAGA`	`12 bp deleted → no frameshift`
164	`GCCGAAAGGACATGGTTATGTTTC------------AGCTTGGCACTCTGGTTGGTAAGA`	`12 bp deleted → no frameshift`
162	`GCCGAAAGGACATGG------------------GTCAGCTTGGCACTCTGGTTGGTAAGA`	`18 bp deleted → no frameshift`
149	`GCCGAAAGGACATGGTTATGT--------------CAGCTTGGCACTCTGGTTGGTAAGA`	`14 bp deleted → frameshift`
141	`GCCGAAAGGACATGGTTATGTTTCT---------------TGGCACTCTGGTTGGTAAGA`	`15 bp deleted → no frameshift`
126	`GCCGAAAGG-----------------------GGTCAGCTTGGCACTCTGGTTGGTAAGA`	`23 bp deleted → frameshift`
123	`GCCGAAAGGACATGGTTATGTTTCTGG----------------------------TAAGA`	`28 bp deleted → frameshift`
123	`GCCGAAAGGACATGG----------------------------CACTCTGGTTGGTAAGA`	`28 bp deleted → frameshift`
120	`GCCGAAAGG------------------------GTCAGCTTGGCACTCTGGTTGGTAAGA`	`24 bp deleted → no frameshift`
115	`GCCGAAAGGACATGGTT------------------------------------GGTAAGA`	`36 bp deleted → no frameshift`
114	`GCCGAAAGG-------------------------TCAGCTTGGCACTCTGGTTGGTAAGA`	`25 bp deleted → frameshift`
110	`GCCGAAAGGACATGGTTATGTTTCTGGATG--------------------GTTGGTAAGA`	`20 bp deleted → frameshift`
99	`GCCGAAAGGACATGGTTATGTTTCT----------------------CTGGTTGGTAAGA`	`22 bp deleted → frameshift`
99	`GCCGAAAGGACATGGTTATG----------------------GCACTCTGGTTGGTAAGA`	`22 bp deleted → frameshift`
90	`GCCGAAAGGACATGGTTATGTTTCTGGATG------------------------GTAAGA`	`24 bp deleted → no frameshift`
86	`GCCGAAAGGACA-------------------------GCTTGGCACTCTGGTTGGTAAGA`	`25 bp deleted → frameshift`
84	`GCCGAAAGGACATGGTTATGTT-------------------------------GGTAAGA`	`31 bp deleted → frameshift`
81	`GCCGAAAGGACATGGTTATGTTT------------------GGCACTCTGGTTGGTAAGA`	`18 bp deleted → no frameshift`
81	`GCCGAAAGGACATGGT----------------------------------------AAGA`	`40 bp deleted → frameshift`
77	`GCCGAAAGGACATGGTTATGTT-------------------GGCACTCTGGTTGGTAAGA`	`19 bp deleted → frameshift`
73	`GCCGAAAGG----------------------------------CACTCTGGTTGGTAAGA`	`34 bp deleted → frameshift`
66	`GCCGAAAGGACATGGTTATG------------------------------GTTGGTAAGA`	`30 bp deleted → no frameshift`
66	`GCCGAAAG------------------------------CTTGGCACTCTGGTTGGTAAGA`	`30 bp deleted → no frameshift`
62	`GC-------------------------------------TTGGCACTCTGGTTGGTAAGA`	`37 bp deleted → frameshift`
60	`GCCGAAAGGACATGGTTATGTTTCTGGA--------------------------------`	`32 bp deleted → frameshift`
60	`GCCGAAAGGACATGGTT------------------------GGCACTCTGGTTGGTAAGA`	`24 bp deleted → no frameshift`
57	`GCCGAAAGGACA---------------------------------CTCTGGTTGGTAAGA`	`33 bp deleted → no frameshift`
54	`GCCGAAAGGACATGGTTATG----------------------------------GTAAGA`	`34 bp deleted → frameshift`
52	`GCCGAAAGGACATGGTTATGT-----------------------------------AAGA`	`35 bp deleted → frameshift`
52	`GCCGAAAGGAC-----------------------------------TCTGGTTGGTAAGA`	`35 bp deleted → frameshift`
48	`GCCGAAAGG------------------------------------------TTGGTAAGA`	`42 bp deleted → no frameshift`
48	`GC------------------------------------------ACTCTGGTTGGTAAGA`	`42 bp deleted → no frameshift`
44	`GCCGAAAGGACATGGTTATGTTT------------------------------GGTAAGA`	`30 bp deleted → no frameshift`
40	`GCCGAAAGG----------------------------------------------TAAGA`	`46 bp deleted → frameshift`
31	`GCCGAAAG---------------------------------------------------A`	`51 bp deleted → no frameshift`
28	`GCCGAAAGGACATGGTTA---------------------------------------AGA`	`39 bp deleted → no frameshift`
24	`GCCGAAAGGA--------------------------------------------------`	`50 bp deleted → frameshift`
19	`GCCGA-------------------------------------------------------`	`55 bp deleted → frameshift`
14	`GCCGAA----------------------------------------------------GA`	`52 bp deleted → frameshift`

Version 5.01 - Documentation - Contact us - Downloads/local installation - Citation - License