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Contents:In the table below, Illumina Nextera Handle sequences have been added and highlighted in bold. Primers for the on-target have been added for convenience. The table below is sorted by the CFD off-target score. Sites with very low CFD scores < 0.02 are unlikely to be cleaved, see our study Haeussler et al. 2016, Figure 2.
In the protocol by Matthew Canver, Harvard, two PCRs are run: one PCR to amplify the potential off-target, then a second PCR to extend the handles with Illumina barcodes. Please click here to download the protocol. Alternatively, you can have a look at Fu et al, 2014.
If a primer was not found, the reason is usually that the region around the off-target is too repetitive. To avoid unspecific primers, all repeats are masked for the primer design (not for off-target search). If you think that we should change the parameters here or should use different primer3 settings, please let us know.
| Name | Primer Sequence | Tm | CFD Score |
|---|---|---|---|
| ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52314142_F | TCGTCGGCAGCGTCGTCGATGGACTAGCAGGCAG | 60.2 | 1.00 |
| ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52314142_R | GTCTCGTGGGCTCGGGTCTGAGCTACTAGGTTGCCC | 59.8 | 1.00 |
| mm4_intron_Lmx1b_chr2_33595578_F | Primer3: not found at this Tm | N.d. | 0.64 |
| mm4_intron_Lmx1b_chr2_33595578_R | Primer3: not found at this Tm | N.d. | 0.64 |
| mm4_intergenic_Glycam1|Mucl1_chr15_103588000_F | TCGTCGGCAGCGTCAGTCTTATCAGAGAAGCCAGCT | 58.6 | 0.43 |
| mm4_intergenic_Glycam1|Mucl1_chr15_103588000_R | GTCTCGTGGGCTCGGGGCTTGTTGCATTGGACCAA | 59.6 | 0.43 |
| mm4_exon_Gm13910_chr2_108949150_F | Primer3: not found at this Tm | N.d. | 0.43 |
| mm4_exon_Gm13910_chr2_108949150_R | Primer3: not found at this Tm | N.d. | 0.43 |
| mm4_intergenic_Gm24009|Mir3096_chr5_23707334_F | Primer3: not found at this Tm | N.d. | 0.43 |
| mm4_intergenic_Gm24009|Mir3096_chr5_23707334_R | Primer3: not found at this Tm | N.d. | 0.43 |
| mm4_intergenic_Gm21679|Gm21529_chrY_30772339_F | Primer3: not found at this Tm | N.d. | 0.43 |
| mm4_intergenic_Gm21679|Gm21529_chrY_30772339_R | Primer3: not found at this Tm | N.d. | 0.43 |
| mm4_intergenic_AC201846.1|Gm20910_chrY_20665517_F | Primer3: not found at this Tm | N.d. | 0.43 |
| mm4_intergenic_AC201846.1|Gm20910_chrY_20665517_R | Primer3: not found at this Tm | N.d. | 0.43 |
| mm4_intergenic_1700040F15Rik|Gm21562_chrY_31668535_F | Primer3: not found at this Tm | N.d. | 0.43 |
| mm4_intergenic_1700040F15Rik|Gm21562_chrY_31668535_R | Primer3: not found at this Tm | N.d. | 0.43 |
| mm4_intron_Gal_chr19_3411260_F | Primer3: not found at this Tm | N.d. | 0.39 |
| mm4_intron_Gal_chr19_3411260_R | Primer3: not found at this Tm | N.d. | 0.39 |
| mm4_intergenic_Redrum|Zfp608_chr18_54773368_F | TCGTCGGCAGCGTCCTGTGGCAGCATGTGGGATA | 60.1 | 0.37 |
| mm4_intergenic_Redrum|Zfp608_chr18_54773368_R | GTCTCGTGGGCTCGGTTGGCAATGTCTCTGCCATG | 58.8 | 0.37 |
| mm4_intergenic_Mc4r|Gnal_chr18_67053854_F | TCGTCGGCAGCGTCCCCCTCGACAGAGCAAGATC | 59.8 | 0.26 |
| mm4_intergenic_Mc4r|Gnal_chr18_67053854_R | GTCTCGTGGGCTCGGGTGTTTATTGGTGGCGAGGC | 59.8 | 0.26 |
| mm4_intergenic_AC102758.1|Sox6_chr7_115327442_F | Primer3: not found at this Tm | N.d. | 0.26 |
| mm4_intergenic_AC102758.1|Sox6_chr7_115327442_R | Primer3: not found at this Tm | N.d. | 0.26 |
| mm4_intergenic_Gm24396|Rit2_chr18_30841869_F | Primer3: not found at this Tm | N.d. | 0.25 |
| mm4_intergenic_Gm24396|Rit2_chr18_30841869_R | Primer3: not found at this Tm | N.d. | 0.25 |
| mm4_intron_Hdac4_chr1_92099710_F | TCGTCGGCAGCGTCCTTGCAAGTCATCCTGGCCT | 60.3 | 0.25 |
| mm4_intron_Hdac4_chr1_92099710_R | GTCTCGTGGGCTCGGACAGCTGTTGTGGCTTGTCT | 60.1 | 0.25 |
| mm4_intron_Reep1_chr6_71788507_F | TCGTCGGCAGCGTCATGTGGCATGCTTAGGTGGG | 60.3 | 0.24 |
| mm4_intron_Reep1_chr6_71788507_R | GTCTCGTGGGCTCGGTGCTACACCTCACGTCACAC | 59.9 | 0.24 |
| mm4_intergenic_Lpar5|Chd4_chr6_125090716_F | TCGTCGGCAGCGTCTTCTAGGTTCCTGGGTCCCA | 59.5 | 0.24 |
| mm4_intergenic_Lpar5|Chd4_chr6_125090716_R | GTCTCGTGGGCTCGGCCACTGACATGATTGGCCCA | 60.3 | 0.24 |
| mm4_intergenic_Gm4746|Gm6977_chrX_90678662_F | Primer3: not found at this Tm | N.d. | 0.24 |
| mm4_intergenic_Gm4746|Gm6977_chrX_90678662_R | Primer3: not found at this Tm | N.d. | 0.24 |
| mm4_exon_Gripap1_chrX_7798965_F | TCGTCGGCAGCGTCCTGTGTCGAGAGGGAGCTTG | 60.1 | 0.23 |
| mm4_exon_Gripap1_chrX_7798965_R | GTCTCGTGGGCTCGGCAGGCTGCTATGGACGCTAT | 59.6 | 0.23 |
| mm4_intergenic_Prmt8|Gm23098_chr6_127822615_F | TCGTCGGCAGCGTCCAGGAAACCCACCTCCCATC | 60.0 | 0.23 |
| mm4_intergenic_Prmt8|Gm23098_chr6_127822615_R | GTCTCGTGGGCTCGGGGAGGGCGGTGTTAGAGAAG | 59.8 | 0.23 |
| mm4_intron_Olfr288_chr15_98191149_F | TCGTCGGCAGCGTCGTGTTTGTTGCGGTCCCTTC | 59.9 | 0.23 |
| mm4_intron_Olfr288_chr15_98191149_R | GTCTCGTGGGCTCGGACTGTTCTTTTCTCCCGCCA | 59.5 | 0.23 |
| mm4_intergenic_C230029F24Rik|PCGEM1_chr1_50037122_F | Primer3: not found at this Tm | N.d. | 0.22 |
| mm4_intergenic_C230029F24Rik|PCGEM1_chr1_50037122_R | Primer3: not found at this Tm | N.d. | 0.22 |
| mm4_intergenic_Ahctf1/Gm10518|Cdc42bpa_chr1_179816635_F | Primer3: not found at this Tm | N.d. | 0.21 |
| mm4_intergenic_Ahctf1/Gm10518|Cdc42bpa_chr1_179816635_R | Primer3: not found at this Tm | N.d. | 0.21 |
| mm4_intron_Fkbp6_chr5_135348153_F | Primer3: not found at this Tm | N.d. | 0.21 |
| mm4_intron_Fkbp6_chr5_135348153_R | Primer3: not found at this Tm | N.d. | 0.21 |
| mm4_intergenic_Prkcdbp|Smpd1_chr7_105536043_F | TCGTCGGCAGCGTCTGCAGGTCAAGATTACTTCCTG | 58.0 | 0.21 |
| mm4_intergenic_Prkcdbp|Smpd1_chr7_105536043_R | GTCTCGTGGGCTCGGGGCCAGGCAGAATAAGTGGA | 59.7 | 0.21 |
| mm4_intron_Foxp1_chr6_99188740_F | TCGTCGGCAGCGTCACCAGAAGCCAGATTTCAGCT | 59.6 | 0.21 |
| mm4_intron_Foxp1_chr6_99188740_R | GTCTCGTGGGCTCGGCCTCTGGGGTGACACCATTG | 60.3 | 0.21 |
| mm4_intron_Phyhipl_chr10_70641862_F | Primer3: not found at this Tm | N.d. | 0.20 |
| mm4_intron_Phyhipl_chr10_70641862_R | Primer3: not found at this Tm | N.d. | 0.20 |
| mm4_intergenic_Ostm1|Ostm1/Gm15200_chr10_42693270_F | Primer3: not found at this Tm | N.d. | 0.20 |
| mm4_intergenic_Ostm1|Ostm1/Gm15200_chr10_42693270_R | Primer3: not found at this Tm | N.d. | 0.20 |
| mm4_intergenic_Gm25905|Dcp1b_chr6_118923733_F | TCGTCGGCAGCGTCGGAACCCCGAGTTATGCAGG | 60.4 | 0.20 |
| mm4_intergenic_Gm25905|Dcp1b_chr6_118923733_R | GTCTCGTGGGCTCGGCATGGAAGCTCTGGTGGGAG | 60.1 | 0.20 |
| mm3_intron_Zfp536_chr7_37772411_F | Primer3: not found at this Tm | N.d. | 0.20 |
| mm3_intron_Zfp536_chr7_37772411_R | Primer3: not found at this Tm | N.d. | 0.20 |
| mm4_intergenic_Gm12437|Gm23746_chr4_48828334_F | TCGTCGGCAGCGTCAGCATTGGACCAGTGACCTG | 59.9 | 0.19 |
| mm4_intergenic_Gm12437|Gm23746_chr4_48828334_R | GTCTCGTGGGCTCGGTGGTGAGTCCAACCTCATTCA | 58.9 | 0.19 |
| mm4_intron_Cpped1_chr16_11894701_F | Primer3: not found at this Tm | N.d. | 0.19 |
| mm4_intron_Cpped1_chr16_11894701_R | Primer3: not found at this Tm | N.d. | 0.19 |
| mm4_intron_Pknox2_chr9_36916938_F | Primer3: not found at this Tm | N.d. | 0.18 |
| mm4_intron_Pknox2_chr9_36916938_R | Primer3: not found at this Tm | N.d. | 0.18 |
| mm4_exon_Usp10_chr8_119953297_F | TCGTCGGCAGCGTCATACGCACAGTCCAGGATGC | 60.1 | 0.17 |
| mm4_exon_Usp10_chr8_119953297_R | GTCTCGTGGGCTCGGTCCTGCTTGGTTTTGGTGGT | 60.0 | 0.17 |
| mm3_intergenic_Fam46a|Gm25125_chr9_85432661_F | Primer3: not found at this Tm | N.d. | 0.17 |
| mm3_intergenic_Fam46a|Gm25125_chr9_85432661_R | Primer3: not found at this Tm | N.d. | 0.17 |
| mm4_intergenic_Ctsd|Gm26143_chr7_142408908_F | Primer3: not found at this Tm | N.d. | 0.16 |
| mm4_intergenic_Ctsd|Gm26143_chr7_142408908_R | Primer3: not found at this Tm | N.d. | 0.16 |
| mm4_intron_Nsg2_chr11_32008454_F | Primer3: not found at this Tm | N.d. | 0.16 |
| mm4_intron_Nsg2_chr11_32008454_R | Primer3: not found at this Tm | N.d. | 0.16 |
| mm4_intron_Nsg2_chr11_32008399_F | TCGTCGGCAGCGTCCAGAGAAGCTCCACCCACTC | 59.7 | 0.16 |
| mm4_intron_Nsg2_chr11_32008399_R | GTCTCGTGGGCTCGGTGGATGGGAGAGAGAGGCTG | 60.3 | 0.16 |
| mm4_intron_Nsg2_chr11_32008344_F | Primer3: not found at this Tm | N.d. | 0.16 |
| mm4_intron_Nsg2_chr11_32008344_R | Primer3: not found at this Tm | N.d. | 0.16 |
| mm4_intron_Nsg2_chr11_32008289_F | TCGTCGGCAGCGTCCCTTGAGACACAGGGCATGA | 59.6 | 0.16 |
| mm4_intron_Nsg2_chr11_32008289_R | GTCTCGTGGGCTCGGGAGTGGGTGGAGCTTCTCTG | 59.7 | 0.16 |
| mm4_intergenic_Rph3a|Ptpn11_chr5_121085388_F | TCGTCGGCAGCGTCCACCCCATGAAAACCAAGCG | 60.0 | 0.16 |
| mm4_intergenic_Rph3a|Ptpn11_chr5_121085388_R | GTCTCGTGGGCTCGGCAGGTGGAGTGTGCTTCAGA | 59.6 | 0.16 |
| mm3_intergenic_Actb|Fscn1_chr5_142955077_F | Primer3: not found at this Tm | N.d. | 0.16 |
| mm3_intergenic_Actb|Fscn1_chr5_142955077_R | Primer3: not found at this Tm | N.d. | 0.16 |
| mm3_intergenic_Kcna5|Kcna1_chr6_126558030_F | Primer3: not found at this Tm | N.d. | 0.15 |
| mm3_intergenic_Kcna5|Kcna1_chr6_126558030_R | Primer3: not found at this Tm | N.d. | 0.15 |
| mm4_intron_Ccdc91_chr6_147544433_F | Primer3: not found at this Tm | N.d. | 0.15 |
| mm4_intron_Ccdc91_chr6_147544433_R | Primer3: not found at this Tm | N.d. | 0.15 |
| mm4_intron_Bmp5_chr9_75835974_F | TCGTCGGCAGCGTCACCTCTACTGTTGCCTGTCA | 58.2 | 0.15 |
| mm4_intron_Bmp5_chr9_75835974_R | GTCTCGTGGGCTCGGTGGAAGACTCCCCACATGAA | 58.2 | 0.15 |
| mm4_intron_Srgn_chr10_62513741_F | TCGTCGGCAGCGTCCAGTGAAGGCTCAGACCCTG | 60.0 | 0.15 |
| mm4_intron_Srgn_chr10_62513741_R | GTCTCGTGGGCTCGGATTTGGGGCTCCTGTGGAAG | 59.9 | 0.15 |
| mm4_intron_Lrrn2_chr1_132930346_F | Primer3: not found at this Tm | N.d. | 0.14 |
| mm4_intron_Lrrn2_chr1_132930346_R | Primer3: not found at this Tm | N.d. | 0.14 |
| mm4_intergenic_Rfx3|Glis3_chr19_28214551_F | TCGTCGGCAGCGTCTCACTGCTGGGTTTAGTCACT | 58.9 | 0.14 |
| mm4_intergenic_Rfx3|Glis3_chr19_28214551_R | GTCTCGTGGGCTCGGGTTCTTGAGGCCCCATGGAA | 59.9 | 0.14 |
| mm4_intron_Frmd4a_chr2_4557596_F | TCGTCGGCAGCGTCCAGAGCAGCACTGAAGTGGA | 59.9 | 0.14 |
| mm4_intron_Frmd4a_chr2_4557596_R | GTCTCGTGGGCTCGGTGCTGCTGTGTAGTGTGGAG | 59.9 | 0.14 |
| mm4_intergenic_Lgals7|Capn12_chr7_28871484_F | Primer3: not found at this Tm | N.d. | 0.14 |
| mm4_intergenic_Lgals7|Capn12_chr7_28871484_R | Primer3: not found at this Tm | N.d. | 0.14 |
| mm4_intron_Gm26697_chr2_126541538_F | Primer3: not found at this Tm | N.d. | 0.14 |
| mm4_intron_Gm26697_chr2_126541538_R | Primer3: not found at this Tm | N.d. | 0.14 |
| mm4_intergenic_Serp2|Lacc1_chr14_76794101_F | Primer3: not found at this Tm | N.d. | 0.14 |
| mm4_intergenic_Serp2|Lacc1_chr14_76794101_R | Primer3: not found at this Tm | N.d. | 0.14 |
| mm4_intergenic_Ipcef1|Cnksr3_chr10_7055136_F | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_intergenic_Ipcef1|Cnksr3_chr10_7055136_R | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_intergenic_Gm24905|Traf1_chr2_34924403_F | TCGTCGGCAGCGTCCTGGTCCCTTAGGGCATTGG | 60.1 | 0.13 |
| mm4_intergenic_Gm24905|Traf1_chr2_34924403_R | GTCTCGTGGGCTCGGACACCTCTGGATGCTGCAAA | 59.8 | 0.13 |
| mm4_intergenic_Tnfsf13b|Myo16_chr8_10196563_F | TCGTCGGCAGCGTCAGTGATGCACGCTTTCTCCA | 59.9 | 0.13 |
| mm4_intergenic_Tnfsf13b|Myo16_chr8_10196563_R | GTCTCGTGGGCTCGGGCATGTTCTGAGCCCCTCTT | 60.0 | 0.13 |
| mm4_intron_Aim2_chr1_173407423_F | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_intron_Aim2_chr1_173407423_R | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_intron_St6galnac3_chr3_153502678_F | TCGTCGGCAGCGTCAGGGGTTTCTGTTGCCTCAG | 59.8 | 0.13 |
| mm4_intron_St6galnac3_chr3_153502678_R | GTCTCGTGGGCTCGGGACAGCATCTGGACAAGCCT | 60.0 | 0.13 |
| mm4_intron_Prom1_chr5_44001715_F | TCGTCGGCAGCGTCACGATGGAAAAGGGACAGCC | 60.3 | 0.12 |
| mm4_intron_Prom1_chr5_44001715_R | GTCTCGTGGGCTCGGACGAGTGAGTGTGGGGTTTC | 59.8 | 0.12 |
| mm4_intron_Tango6_chr8_106726093_F | Primer3: not found at this Tm | N.d. | 0.12 |
| mm4_intron_Tango6_chr8_106726093_R | Primer3: not found at this Tm | N.d. | 0.12 |
| mm4_intergenic_Hs3st5|Hdac2_chr10_36956418_F | Primer3: not found at this Tm | N.d. | 0.11 |
| mm4_intergenic_Hs3st5|Hdac2_chr10_36956418_R | Primer3: not found at this Tm | N.d. | 0.11 |
| mm4_intergenic_Gm16619|Cd47_chr16_49818906_F | Primer3: not found at this Tm | N.d. | 0.11 |
| mm4_intergenic_Gm16619|Cd47_chr16_49818906_R | Primer3: not found at this Tm | N.d. | 0.11 |
| mm4_exon_Slc5a3_chr16_92082313_F | TCGTCGGCAGCGTCCCAGCTCGAAGGATCCACTC | 59.8 | 0.11 |
| mm4_exon_Slc5a3_chr16_92082313_R | GTCTCGTGGGCTCGGGACACCCGTAAGAGTCACGG | 60.1 | 0.11 |
| mm4_intron_Sulf1_chr1_12769441_F | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intron_Sulf1_chr1_12769441_R | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intron_Atxn1_chr13_45656080_F | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intron_Atxn1_chr13_45656080_R | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intron_Pde4b_chr4_102582016_F | TCGTCGGCAGCGTCGCTCTACAAATCTGAGCAAATACGT | 59.7 | 0.09 |
| mm4_intron_Pde4b_chr4_102582016_R | GTCTCGTGGGCTCGGGGAGACTTCAGACTTCTCCAGG | 59.5 | 0.09 |
| mm4_intergenic_Gm26184|Dlgap2_chr8_14352781_F | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intergenic_Gm26184|Dlgap2_chr8_14352781_R | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intron_Rgs17_chr10_5875876_F | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intron_Rgs17_chr10_5875876_R | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intron_Lmx1a_chr1_167789811_F | TCGTCGGCAGCGTCGGCCATACAGCACACTCCTT | 60.0 | 0.09 |
| mm4_intron_Lmx1a_chr1_167789811_R | GTCTCGTGGGCTCGGAGCTTTGAGTGGAGGAAGCC | 59.9 | 0.09 |
| mm4_intron_Pdzrn4_chr15_92577095_F | TCGTCGGCAGCGTCTAATGGCAGGACAGTGACCT | 58.3 | 0.08 |
| mm4_intron_Pdzrn4_chr15_92577095_R | GTCTCGTGGGCTCGGCCCGTGTGGAACTTTCAGCA | 60.8 | 0.08 |
| mm3_intron_Styxl1_chr5_135752561_F | Primer3: not found at this Tm | N.d. | 0.08 |
| mm3_intron_Styxl1_chr5_135752561_R | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intron_Cst9_chr2_148838034_F | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intron_Cst9_chr2_148838034_R | Primer3: not found at this Tm | N.d. | 0.08 |
| mm3_intron_Ptges_chr2_30892991_F | Primer3: not found at this Tm | N.d. | 0.08 |
| mm3_intron_Ptges_chr2_30892991_R | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intron_Cntnap5a_chr1_115881552_F | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intron_Cntnap5a_chr1_115881552_R | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_exon_Atg7_chr6_114860269_F | TCGTCGGCAGCGTCCACTGACGGGTATCTGAGGC | 59.8 | 0.07 |
| mm4_exon_Atg7_chr6_114860269_R | GTCTCGTGGGCTCGGACTCCATGACAACACTGCGT | 59.8 | 0.07 |
| mm4_intergenic_4930594M17Rik|Gm26012_chr18_81660907_F | TCGTCGGCAGCGTCGGACCAGGAAAGGAAGCAGG | 60.3 | 0.07 |
| mm4_intergenic_4930594M17Rik|Gm26012_chr18_81660907_R | GTCTCGTGGGCTCGGTTTGCAGCCTCTCCTTCCTG | 59.9 | 0.07 |
| mm3_intergenic_Gm11266|Gm11267_chr4_82575410_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm3_intergenic_Gm11266|Gm11267_chr4_82575410_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intron_Crebbp_chr16_4213055_F | TCGTCGGCAGCGTCCACTTTTCCTCCCGACCAGG | 60.3 | 0.06 |
| mm4_intron_Crebbp_chr16_4213055_R | GTCTCGTGGGCTCGGTCTCCGCGAATGACAACACA | 59.9 | 0.06 |
| mm4_intergenic_Irx5|Irx6_chr8_92633997_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_Irx5|Irx6_chr8_92633997_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_Gm11850|Gm11849_chr4_17099657_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_Gm11850|Gm11849_chr4_17099657_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intron_Sfrp1_chr8_23418041_F | TCGTCGGCAGCGTCTGTGACAGAGAGGGGCTAGC | 60.9 | 0.05 |
| mm4_intron_Sfrp1_chr8_23418041_R | GTCTCGTGGGCTCGGCTCCACAAGGTCACAGCTCA | 59.6 | 0.05 |
| mm4_intergenic_Rxrg|Lmx1a_chr1_167641479_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_Rxrg|Lmx1a_chr1_167641479_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_Phkb|Gm23904_chr8_86230900_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_Phkb|Gm23904_chr8_86230900_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_Gm25377|Hs3st1_chr5_39574874_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_Gm25377|Hs3st1_chr5_39574874_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intron_Bre_chr5_31981828_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intron_Bre_chr5_31981828_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm3_exon_Zfp688_chr7_127421543_F | TCGTCGGCAGCGTCGAGAGAACAGGGGTCACAGTG | 59.9 | 0.04 |
| mm3_exon_Zfp688_chr7_127421543_R | GTCTCGTGGGCTCGGCATTCAGGCAAGAGCATCGC | 59.9 | 0.04 |
| mm4_intergenic_Gm12160|Gm12161_chr11_45214361_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Gm12160|Gm12161_chr11_45214361_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_3930402G23Rik|Irs2_chr8_10966958_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_3930402G23Rik|Irs2_chr8_10966958_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Epha5|Gm24626_chr5_84483638_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Epha5|Gm24626_chr5_84483638_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intron_Tmem132d_chr5_128359598_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intron_Tmem132d_chr5_128359598_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Tm4sf20|Gm22396/Agfg1_chr1_82826149_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Tm4sf20|Gm22396/Agfg1_chr1_82826149_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_exon_Cd209b_chr8_3918195_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_exon_Cd209b_chr8_3918195_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Arfip1|Tigd4_chr3_84582699_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Arfip1|Tigd4_chr3_84582699_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Gm17566|Gm23153_chr1_190326942_F | TCGTCGGCAGCGTCAGCTGCCTCTGAACACACTG | 60.2 | 0.04 |
| mm4_intergenic_Gm17566|Gm23153_chr1_190326942_R | GTCTCGTGGGCTCGGACCGGGGCAAGATTCCATTT | 59.9 | 0.04 |
| mm3_intron_Lhcgr_chr17_88786622_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm3_intron_Lhcgr_chr17_88786622_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intron_Cdc73_chr1_143636289_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intron_Cdc73_chr1_143636289_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Gm4954|Gm6286_chr1_166894993_F | TCGTCGGCAGCGTCCCACGAAGGAAGTGCCATCT | 60.0 | 0.03 |
| mm4_intergenic_Gm4954|Gm6286_chr1_166894993_R | GTCTCGTGGGCTCGGCATACAGATGCACAGGGGCA | 60.1 | 0.03 |
| mm4_intergenic_Maml3|Foxo1/Gm20402_chr3_52244954_F | TCGTCGGCAGCGTCGCTTGAGCCATTTTGGTGGG | 60.0 | 0.03 |
| mm4_intergenic_Maml3|Foxo1/Gm20402_chr3_52244954_R | GTCTCGTGGGCTCGGAGCCCCTAGCCCACAATTTT | 59.5 | 0.03 |
| mm3_intergenic_Stac2|Gm11631_chr11_98054063_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm3_intergenic_Stac2|Gm11631_chr11_98054063_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Tmtc4|Nalcn_chr14_123275801_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Tmtc4|Nalcn_chr14_123275801_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Gm11501|Gm25113_chr11_92858516_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Gm11501|Gm25113_chr11_92858516_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Gm25779|Gm13173_chr4_153022719_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Gm25779|Gm13173_chr4_153022719_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intron_Prdm11_chr2_93006121_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intron_Prdm11_chr2_93006121_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intron_Wnk2_chr13_49052471_F | TCGTCGGCAGCGTCTTACACCAACCCAGAGCCAG | 59.6 | 0.02 |
| mm4_intron_Wnk2_chr13_49052471_R | GTCTCGTGGGCTCGGCTTCTGTGGGAGGGCCATAC | 59.8 | 0.02 |
| mm4_intergenic_Gm23120|5S_rRNA_chr11_107851514_F | TCGTCGGCAGCGTCGACTTCCCAAGCCTCTGACC | 60.0 | 0.02 |
| mm4_intergenic_Gm23120|5S_rRNA_chr11_107851514_R | GTCTCGTGGGCTCGGAAGTCTGGACCAAAGCGAGG | 59.9 | 0.02 |
| mm4_intergenic_Ptgdr/Gm16976|Gm16976_chr14_44902170_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Ptgdr/Gm16976|Gm16976_chr14_44902170_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Gm17070|Ptgdr_chr14_44813086_F | TCGTCGGCAGCGTCCCTCTCATGCTGCCATGGAA | 60.1 | 0.02 |
| mm4_intergenic_Gm17070|Ptgdr_chr14_44813086_R | GTCTCGTGGGCTCGGAGGAATGCTAGAACACAGAGCT | 59.1 | 0.02 |
| mm4_intergenic_Dcc|Gm25509_chr18_72665196_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Dcc|Gm25509_chr18_72665196_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Wwc2|Dctd_chr8_48049071_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Wwc2|Dctd_chr8_48049071_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intron_D430042O09Rik_chr7_125836260_F | TCGTCGGCAGCGTCCAGCAAGAGACTGGTGAGCA | 59.9 | 0.02 |
| mm4_intron_D430042O09Rik_chr7_125836260_R | GTCTCGTGGGCTCGGTAAGATCATGGGGCCTCAGC | 59.2 | 0.02 |
| mm3_intron_Hoxa10_chr6_52236799_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm3_intron_Hoxa10_chr6_52236799_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_mmu-mir-6347|Tmco1_chr1_167304541_F | TCGTCGGCAGCGTCTCTTGTCGCTCTGCATGTGT | 59.9 | 0.02 |
| mm4_intergenic_mmu-mir-6347|Tmco1_chr1_167304541_R | GTCTCGTGGGCTCGGGCCACACATCTCCGTCATCA | 60.1 | 0.02 |
| mm4_exon_P2rx1_chr11_72999261_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_exon_P2rx1_chr11_72999261_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intron_Gng12_chr6_66915104_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intron_Gng12_chr6_66915104_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm3_intron_Camk1d_chr2_5711705_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm3_intron_Camk1d_chr2_5711705_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_Msi2_chr11_88508099_F | TCGTCGGCAGCGTCTTTGCAGCTAGACACCTGGG | 59.9 | 0.01 |
| mm4_intron_Msi2_chr11_88508099_R | GTCTCGTGGGCTCGGCATGGCCACCACCTTTCAGA | 60.2 | 0.01 |
| mm4_intron_Npc1_chr18_12212134_F | TCGTCGGCAGCGTCGTGCTACGTCTGCCTTCACT | 60.0 | 0.01 |
| mm4_intron_Npc1_chr18_12212134_R | GTCTCGTGGGCTCGGTGTTTGCGTAAGTCAGGGGA | 59.2 | 0.01 |
| mm3_intergenic_Zfp69|Smap2_chr4_120965433_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm3_intergenic_Zfp69|Smap2_chr4_120965433_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_1700025G04Rik_chr1_151996153_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_1700025G04Rik_chr1_151996153_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Gm5138|Zfml_chr6_83914298_F | TCGTCGGCAGCGTCGGTGTAGGACCAGCCATAGC | 59.8 | 0.00 |
| mm4_intergenic_Gm5138|Zfml_chr6_83914298_R | GTCTCGTGGGCTCGGATCTTCGCCTAAAGCCCTCG | 59.8 | 0.00 |
| mm4_intron_Pappa2_chr1_158956369_F | TCGTCGGCAGCGTCTGGTGCCTACACTGCTTCTG | 59.9 | 0.00 |
| mm4_intron_Pappa2_chr1_158956369_R | GTCTCGTGGGCTCGGGGTACATAGAGAACAGACAGGGG | 59.6 | 0.00 |
| mm4_intergenic_Sult2b1|Lmtk3_chr7_45778579_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Sult2b1|Lmtk3_chr7_45778579_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Gpr20_chr15_73706612_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Gpr20_chr15_73706612_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Cep350_chr1_155972753_F | TCGTCGGCAGCGTCAGGTCTCAGGATGACCCTCC | 60.0 | 0.00 |
| mm4_intron_Cep350_chr1_155972753_R | GTCTCGTGGGCTCGGCTCGGATGTCTCTGTCCGC | 59.9 | 0.00 |
| mm4_intron_Zc3h18_chr8_122398518_F | TCGTCGGCAGCGTCAGCCATCTCAGACCCAGTCT | 59.9 | 0.00 |
| mm4_intron_Zc3h18_chr8_122398518_R | GTCTCGTGGGCTCGGTTAGGGTAGCGGCCAGAAAC | 59.7 | 0.00 |
| mm4_intron_Uvrag_chr7_99015310_F | TCGTCGGCAGCGTCCTCTAGCGTGGAGGGAGGAT | 60.1 | 0.00 |
| mm4_intron_Uvrag_chr7_99015310_R | GTCTCGTGGGCTCGGATGACCTTCATCCGCACACC | 60.3 | 0.00 |
| mm4_intron_Thsd4_chr9_60108539_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Thsd4_chr9_60108539_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Skint1_chr4_112022355_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Skint1_chr4_112022355_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Pex1_chr5_3609522_F | TCGTCGGCAGCGTCCAAGCGTCCTTGCTGCATTT | 60.0 | 0.00 |
| mm4_intron_Pex1_chr5_3609522_R | GTCTCGTGGGCTCGGTTTCTGTCTGCCCAGCCAAA | 60.1 | 0.00 |
| mm4_intron_Pde6a_chr18_61273304_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Pde6a_chr18_61273304_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Mvb12b_chr2_33778293_F | TCGTCGGCAGCGTCTTTCTACCTGTGCCATGCCC | 60.3 | 0.00 |
| mm4_intron_Mvb12b_chr2_33778293_R | GTCTCGTGGGCTCGGAATGGCCCTCTTCCATGAGC | 60.1 | 0.00 |
| mm4_intron_Maff_chr15_79352751_F | TCGTCGGCAGCGTCGGAGTTTCAGTCCTGGGCTC | 60.0 | 0.00 |
| mm4_intron_Maff_chr15_79352751_R | GTCTCGTGGGCTCGGCAGGACTAACTCTTGACCTACCA | 58.9 | 0.00 |
| mm4_intron_Ctnnd2_chr15_30879341_F | TCGTCGGCAGCGTCCCTGTGTTGTACTGCATCCCT | 59.9 | 0.00 |
| mm4_intron_Ctnnd2_chr15_30879341_R | GTCTCGTGGGCTCGGTTTCTTCTGCTGGCCCCAAA | 60.1 | 0.00 |
| mm4_intron_Cntfr_chr4_41672424_F | TCGTCGGCAGCGTCCAGGACTGCTTGTCTCTGGG | 60.0 | 0.00 |
| mm4_intron_Cntfr_chr4_41672424_R | GTCTCGTGGGCTCGGCAAGAGGGAGGCAGACACAG | 60.0 | 0.00 |
| mm4_intergenic_Tmem173|Gm16490_chr18_35744307_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Tmem173|Gm16490_chr18_35744307_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Rbfox3|Enpp7_chr11_118957255_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Rbfox3|Enpp7_chr11_118957255_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_RP24-88B13.1|Ets1_chr9_32631514_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_RP24-88B13.1|Ets1_chr9_32631514_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_RP23-304I16.1/Kcnn2|RP23-304I16.1_chr18_45697593_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_RP23-304I16.1/Kcnn2|RP23-304I16.1_chr18_45697593_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Pole4|Hk2_chr6_82719256_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Pole4|Hk2_chr6_82719256_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Pole4|Hk2_chr6_82718574_F | TCGTCGGCAGCGTCACCAGAGGGCATGAGAGATG | 58.8 | 0.00 |
| mm4_intergenic_Pole4|Hk2_chr6_82718574_R | GTCTCGTGGGCTCGGGCTTCCTCCTTGACTGGGTC | 60.0 | 0.00 |
| mm4_intergenic_Mfsd4/Gm26892|Gm26034_chr1_132098428_F | TCGTCGGCAGCGTCTGGCACAGAGGACCAAATGT | 59.5 | 0.00 |
| mm4_intergenic_Mfsd4/Gm26892|Gm26034_chr1_132098428_R | GTCTCGTGGGCTCGGGTTTCCAGACTTCAGCCGGA | 59.9 | 0.00 |
| mm4_intergenic_Fat4|Gm9845_chr3_39173398_F | TCGTCGGCAGCGTCTCCATGTTGGTAGACAAGCAT | 57.2 | 0.00 |
| mm4_intergenic_Fat4|Gm9845_chr3_39173398_R | GTCTCGTGGGCTCGGGGCTAGAGTCCATGTTGGCA | 59.7 | 0.00 |
| mm4_intergenic_2010007H06Rik|1810046K07Rik_chr9_51283137_F | TCGTCGGCAGCGTCGCTTTCCACCCAAGCCTTTG | 59.9 | 0.00 |
| mm4_intergenic_2010007H06Rik|1810046K07Rik_chr9_51283137_R | GTCTCGTGGGCTCGGTGTGGCATGGAAAAGCAGAGA | 60.2 | 0.00 |
| mm4_exon_Zc3h13_chr14_75330586_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_exon_Zc3h13_chr14_75330586_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_exon_Agbl4_chr4_111618974_F | TCGTCGGCAGCGTCGCGCATGGCTTCCCAAAATC | 60.8 | 0.00 |
| mm4_exon_Agbl4_chr4_111618974_R | GTCTCGTGGGCTCGGGGTGTGGTGTGGAGTTGATCT | 59.9 | 0.00 |
| mm4_exon_1110008J03Rik_chr5_120611449_F | TCGTCGGCAGCGTCCTTGGTGTGAGGTTTGGGGT | 60.1 | 0.00 |
| mm4_exon_1110008J03Rik_chr5_120611449_R | GTCTCGTGGGCTCGGAACCAGACTTTGACCCACCC | 59.8 | 0.00 |
| mm3_intron_Rgs7_chr1_175333225_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm3_intron_Rgs7_chr1_175333225_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm3_intron_Fam110b_chr4_5662358_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm3_intron_Fam110b_chr4_5662358_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm3_intron_Camkmt_chr17_85271762_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm3_intron_Camkmt_chr17_85271762_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm3_exon_Pitpnm1_chr19_4113556_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm3_exon_Pitpnm1_chr19_4113556_R | Primer3: not found at this Tm | N.d. | 0.00 |
These only list off-targets that have primers in the table above. Primers underlined, off-targets in bold.
| ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52314142 |
GTCGATGGACTAGCAGGCAGTGCTGCAGGGCTGGGCGCCGAGCCACGGTCTGCTGGAGCGGCCATGCTTGGCCCGGGACC CCCAGTCCCCTCCGCGGACAGCCTCTCTGGCCAAGGGCAACCTAGTAGCTCAGAC |
| mm4_intergenic_Glycam1|Mucl1_chr15_103588000 |
AGTCTTATCAGAGAAGCCAGCTCCCAGGGCAGCCTCTCTAGCCACATTGCTTGTTTTTACTTCCTGAATAACTGTACCAA AGATGATATAATCAACAACATCCTTTGGAATATTGGTCCAATGCAACAAGCC |
| mm4_intergenic_Redrum|Zfp608_chr18_54773368 |
CTGTGGCAGCATGTGGGATATTTACTGTCAGGTAGCTTATGTCAAATGTGCCAGAAAAAAAAAAGCCAGCACATCTATCA TAACAAACAATATCTGCAAGAGAGGCTGTCCATGGGGCATGGCAGAGACATTGCCAA |
| mm4_intergenic_Mc4r|Gnal_chr18_67053854 |
CCCCTCGACAGAGCAAGATCTATGGCGATGCTTCTCATCCCAACTCCACTCAGATTCTCAGGCATCCATCCCCCTGCCAG CCCCCCTGGACAGCTTCTCTGTGCAAGGTTCTGCCTCGCCACCAATAAACAC |
| mm4_intron_Hdac4_chr1_92099710 |
CTTGCAAGTCATCCTGGCCTGCCATGACTGGCCCAGGCTGGCCAGGCCAAGTGTGAGCTTCCAGGGCCAGCTGCGGGGCC AAGGACTGCCTTCCTGGCCAACAAGACAAGCCACAACAGCTGT |
| mm4_intron_Reep1_chr6_71788507 |
ATGTGGCATGCTTAGGTGGGGCTGCTGGGCCCTGCTGTCGAGCACGTTCGGCTGGAGAGGCTGTCAGCGGGCTGAGTGTG ACGTGAGGTGTAGCA |
| mm4_intergenic_Lpar5|Chd4_chr6_125090716 |
TTCTAGGTTCCTGGGTCCCATGGAGTGGAAACAGAACTCTACATAACTGGCTAGAGAGGCTGCCCCTGGGGTGCATTAAT TAGGAGATTATACTATCCTTAGTCTCCGTGGGCCAATCATGTCAGTGG |
| mm4_exon_Gripap1_chrX_7798965 |
CTGTGTCGAGAGGGAGCTTGGATCCAGGGATCTGGGATATTGTTCCACAGATGAAGGGTATATAGGTATGCCAGTGAGGC TGTCCATGGTGGTAATAGCGTCCATAGCAGCCTG |
| mm4_intergenic_Prmt8|Gm23098_chr6_127822615 |
CAGGAAACCCACCTCCCATCTAGAGCTGTCCAGGGACAGCCTCCCTGGCCTCTGCTTCACTTTGGAGGCTGTGCCTCTGG AGATAGGGTAGACTTAAATCAGGCTGGACCTGTGCTTCTCTAACACCGCCCTCC |
| mm4_intron_Olfr288_chr15_98191149 |
GTGTTTGTTGCGGTCCCTTCATGAGGCGATTTAATCCCCTTGATGACTTGCTGGTAAGTTGTATTGGACAACGAGGCTGC CCGTGGCTTGGCGGGAGAAAAGAACAGT |
| mm4_intergenic_Prkcdbp|Smpd1_chr7_105536043 |
TGCAGGTCAAGATTACTTCCTGCTACTCTGCCTGGGACAGCCTCCCTGGCTATAAACAAAGGCTCCACACAATTTGACTT ACTCTGCCTTTCCTATACTTCCACTTATTCTGCCTGGCC |
| mm4_intron_Foxp1_chr6_99188740 |
ACCAGAAGCCAGATTTCAGCTATTTGGACTATAGTCCTGTCTCCTTGGACAGCCTTCCTGGACAACTTTAAGGTGCAGAA TACAAACCAAGTTTGTCCAAAGTCCACAGACCATGACAAACAATGGTGTCACCCCAGAGG |
| mm4_intergenic_Gm25905|Dcp1b_chr6_118923733 |
GGAACCCCGAGTTATGCAGGAGGGGCCCTGGGAGCACAGCCCTGAGCCGGTCTCCTTAATAAAGTCAGGCACACAAGCTC CTCTGGCCACTCCCCACGGACAGCCCAACTGACCAAACTCCCACCAGAGCTTCCATG |
| mm4_intergenic_Gm12437|Gm23746_chr4_48828334 |
AGCATTGGACCAGTGACCTGCCTATTACCTCTGCACTATATTTACAACCAAATTAAGGAGAAGCATAGAACCCTTTTGCA AAAGAGGCTGTCCCTGGCAAAATGAATGAGGTTGGACTCACCA |
| mm4_exon_Usp10_chr8_119953297 |
ATACGCACAGTCCAGGATGCGCTGGAGAGCTTGGTGGCCAGGGAGTCTGTCCAAGGTTACACCACCAAAACCAAGCAGGA |
| mm4_intron_Nsg2_chr11_32008399 |
CAGAGAAGCTCCACCCACTCCCCAGGACAGCCTCTCTCTCCCATCCATCCCTCTACAGAGAAGCTCCACCCACTCCCCAG GACAGCCTCTCTCTCCCATCCA |
| mm4_intron_Nsg2_chr11_32008289 |
CCTTGAGACACAGGGCATGAGAAATGTCAGCCTCTCTCCAGGGAGAAGCTCCACCCACTCCCCAGGACAGCCTCTCTCTC CCATCCATTCCTCTACAGAGAAGCTCCACCCACTC |
| mm4_intergenic_Rph3a|Ptpn11_chr5_121085388 |
CACCCCATGAAAACCAAGCGAGCTGCCACATGGTGGCTGCCCACGGACAGGCTCTCAAGCCACAGCCTGAGTGTTAAGAT GGAGTTTGGAAGGAGAGTTCTCTCAGCTCTGAAGCACACTCCACCTG |
| mm4_intron_Bmp5_chr9_75835974 |
ACCTCTACTGTTGCCTGTCATCCACTGATAGCCTCACTGCCCAAGGATCTCATAGGCTTTCTTTCTCCCTGTTTTCCTCA GTACTTTATATTAGTGAACTCTCTGTTTCATGTGGGGAGTCTTCCA |
| mm4_intron_Srgn_chr10_62513741 |
CAGTGAAGGCTCAGACCCTGAGGAACCTCCTCTGACAGCTTCCCTGTCCAATGCTAGCCTTTTCAGCTTCCACAGGAGCC CCAAAT |
| mm4_intergenic_Rfx3|Glis3_chr19_28214551 |
TCACTGCTGGGTTTAGTCACTACAACTTTAAGAGGAGAATTTGCCAGAGAGGTTGTCAAGGGTAAGGGAAATAGTGTCTG TCCTTTCCATGGGGCCTCAAGAAC |
| mm4_intron_Frmd4a_chr2_4557596 |
CAGAGCAGCACTGAAGTGGAAGCCCCACCCCTTTCCCTGGACAGCCACCCTGGCTAAAGATGAGGGGCTGCAGCACGCCC AGATGGTTCTATTTGGGCCTTTGGCAGACACACGGATCTCCACACTACACAGCAGCA |
| mm4_intergenic_Gm24905|Traf1_chr2_34924403 |
CTGGTCCCTTAGGGCATTGGCAGACTCACACCTATTGAGCCTGTCATATGGTCACACCTGTTGGGTCTATTGTATGGTAT TCCCTAGACCTTGGTAAGAGAGGCTGTACCAGGGCTTTTGCAGCATCCAGAGGTGT |
| mm4_intergenic_Tnfsf13b|Myo16_chr8_10196563 |
AGTGATGCACGCTTTCTCCACCGAGGACAGACTCTCTGGCTGATGCTGTCCACACCGTAAGAATCCCCAGAAACCATAAT CTCAATCACTATACCTGTGTCAAGCATAAGAGGGGCTCAGAACATGC |
| mm4_intron_St6galnac3_chr3_153502678 |
AGGGGTTTCTGTTGCCTCAGCAATACAGACCATTTTACCTGCTAATTATATTATGACTGCATGATTTATCAGCTGGGTAT GTATGGCCAGAGAGGCAGTAAGTGGTCAGGCTTGTCCAGATGCTGTC |
| mm4_intron_Prom1_chr5_44001715 |
ACGATGGAAAAGGGACAGCCCGTTGCTTAAGCAGAAGCCAAGTGTTTCTCATGAGAACTTGGCTTGGGAGGCTGTCTGGG GACAGTGATGCAGAGAAATGCCTGGCCCTCAAGAAACCCCACACTCACTCGT |
| mm4_exon_Slc5a3_chr16_92082313 | CCAGCTCGAAGGATCCACTCTTGCACAGAGAGGCTGACCTGGGTAGTCTATCCGTGACTCTTACGGGTGTC |
| mm4_intron_Pde4b_chr4_102582016 |
GCTCTACAAATCTGAGCAAATACGTATACAACACAGATATTCTTAGGTCAGACAGGCTGTCTGTGGTTATGCCAAGCTTT GGTCAATACCTGGAGAAGTCTGAAGTCTCC |
| mm4_intron_Lmx1a_chr1_167789811 |
GGCCATACAGCACACTCCTTCCAATCCTGCAGATCGCATCCTGTCCTGGGAGGCTGTCCGTGGGTGGCTCCAGGATCCAT GGCAGATGACCTTGTTAATCTTTTTCAAGCTTTGGCTTCCTCCACTCAAAGCT |
| mm4_intron_Pdzrn4_chr15_92577095 |
TAATGGCAGGACAGTGACCTGGACCCTGGCTAGAGAGGCTGGCCATGGCCAGGAGTGTGTAGATTGATGCATTTAACCTT ACCATGCTGAAAGTTCCACACGGG |
| mm4_exon_Atg7_chr6_114860269 |
CACTGACGGGTATCTGAGGCCCGTTGCCCACAGAGCCAGTCCGCGGTGCGGTTGTTTTCCTGTGACAACCTCAGTAGCCT GTAGAATAACAAACTAGTGGCTATTAATGCGGACGCAGTGTTGTCATGGAGT |
| mm4_intergenic_4930594M17Rik|Gm26012_chr18_81660907 |
GGACCAGGAAAGGAAGCAGGAAGAACAGCTCAGAAGCCAACCTTAGGCCAGGGAGTCTGTCCCTGGAAATGCCCTGAAAG CAGAAGAGATGGAGAGAAAGCTAACTGGATCCTCTCACAGGAAGGAGAGGCTGCAAA |
| mm4_intron_Crebbp_chr16_4213055 |
CACTTTTCCTCCCGACCAGGGAAGCGCATCCCAAGGCCCGGGACAGAGTCTCTGGCCACTGCGACCCTCAACCCCCTCCC TGCCCCGAACTCTGACAGCGAGCCTAGACCCAGCCTCACCTGTGTTGTCATTCGCGGAGA |
| mm4_intron_Sfrp1_chr8_23418041 |
TGTGACAGAGAGGGGCTAGCCTGGGCTAGGGAGCAAATCCAGGCCCCTCCTAGGATGGCCACTCTGGCCAACAGGAAAAC CCAGTGACTTCTGACCTCTGAGGACATAAGTATGAGCTGTGACCTTGTGGAG |
| mm3_exon_Zfp688_chr7_127421543 |
GAGAGAACAGGGGTCACAGTGCTGGCCTGAGAGGCTGGCCGGGGCAGATAGCATCCCACTAGGGTGAGGGCACCTCCTCG GAACTTATGCACACATTAACCTGGACAGGGTTTGCGATGCTCTTGCCTGAATG |
| mm4_intergenic_Gm17566|Gm23153_chr1_190326942 |
AGCTGCCTCTGAACACACTGCCTCTGACATCCTCTCTGGGAAAGTCTGATTTTATCATCAAAAAACCCCAGTCCTCAAAA CTTGTATCCTTCCTTCTCCAAATGGAATCTTGCCCCGGT |
| mm4_intergenic_Gm4954|Gm6286_chr1_166894993 |
CCACGAAGGAAGTGCCATCTCATTCATCAGGGAGAAGTAGGTCATGTGGCCCTACCTAATGGCCACAGAGGCTGTGAGTG GTAGAATAGTGAGGTAGCCATCTGCCCCTGTGCATCTGTATG |
| mm4_intergenic_Maml3|Foxo1/Gm20402_chr3_52244954 |
GCTTGAGCCATTTTGGTGGGGCCTGGACCAGATCCTACCAGATGTAAATCTGGTGATGTAGTTTGGGTGTCTCGGTTGTC CAGAGATGATGTCCTTGGTCCTAAAAATTGTGGGCTAGGGGCT |
| mm4_intron_Wnk2_chr13_49052471 |
TTACACCAACCCAGAGCCAGCCTCTCAGGCTAACTTAGGGTCCCCCGGCAGCCTCTCAGGCTAACTTAGGGTCCCCCGGC ACCATCCCTCCTCAGTCCAGGTATGGCCCTCCCACAGAAG |
| mm4_intergenic_Gm23120|5S_rRNA_chr11_107851514 |
GACTTCCCAAGCCTCTGACCCATTTAATCCATCCTCGCACGGCTTCTCTGGCCTAGAATGTCCCTCGCTTTGGTCCAGAC TT |
| mm4_intergenic_Gm17070|Ptgdr_chr14_44813086 |
CCTCTCATGCTGCCATGGAAACAGAAGTCCCAGGCTGTTGGGCAGTTGTTCCCCTTACTGTCCATGGCAGAGACTCCAAG GCCTGCCTCTCTGTCCAATCCTTTCTTCCCTCAGTAGCTCTGTGTTCTAGCATTCCT |
| mm4_intron_D430042O09Rik_chr7_125836260 |
CAGCAAGAGACTGGTGAGCAGGGGGTTGATCATTAGCATAAGGCTGAGATGTTGGCCTCAGAGGCTGGCCTAGGCCAAGC TTTGCTAGCAGGACAGGTTCAAACTTAGGATGGCTGAGGCCCCATGATCTTA |
| mm4_intergenic_mmu-mir-6347|Tmco1_chr1_167304541 |
TCTTGTCGCTCTGCATGTGTAAGGATCACAGTGCCTGCATGGGCAGAGTGGCTGGCCGAGGGAGAAAACTATCCCACACA TGAACACAGTCCTGGTGTGAATTAGACTCGATGATGACGGAGATGTGTGGC |
| mm4_intron_Msi2_chr11_88508099 |
TTTGCAGCTAGACACCTGGGCTGGCCACTGCCAGGCTCTCTGACCAAACAGCTGACAGAGCACATTAGCTCACCTTCTCT GCTTGTCAGAACATTTACGTTCTGAAAGGTGGTGGCCATG |
| mm4_intron_Npc1_chr18_12212134 |
GTGCTACGTCTGCCTTCACTATGGCTGCGTCCTCAGCAATAGTACTGCCTCACACAGGCTCTCAGGCCAACACAATCAGA TCCCCTGACTTACGCAAACA |
| mm4_intergenic_Gm5138|Zfml_chr6_83914298 |
GGTGTAGGACCAGCCATAGCTGGCGATCGCCCCCTTCTGGCAGAGGGCGGCCTTGTCCCCCGACAGCGTCTCTGGAGAAC GCGGAGCCTGAGGCACGCAGGCGCAGTGAGCGGACGAGGGCTTTAGGCGAAGAT |
| mm4_intron_Pappa2_chr1_158956369 |
TGGTGCCTACACTGCTTCTGCACTGATAAATAACCCCCACCCCTCCCCCCCACAGCCCCTCTGGCTAAGCCTTCTTGGCT TTCCTGCCTTTAGGCTGTTCCCCCTGTCTGTTCTCTATGTACC |
| mm4_intron_Cep350_chr1_155972753 |
AGGTCTCAGGATGACCCTCCAGGAAAAGTCTCGGGACCCAAGGAGGGTCCCCGGGGGGGACGTCAGGCAGTGCCAGGGGG GCCCCGCCCCGCCTCTCTGGCCACCTCGGGGACCTGGGACCGCGGACAGAGACATCCGAG |
| mm4_intron_Zc3h18_chr8_122398518 |
AGCCATCTCAGACCCAGTCTCAGGCAAATCTCAGTAGGCCAGAGAGACCCTCCGTGGATGTAGAGTTAACATTTTTAAGT GTTTCTGGCCGCTACCCTAA |
| mm4_intron_Uvrag_chr7_99015310 |
CTCTAGCGTGGAGGGAGGATTTTCCAGAGACAAGGCAAGTGCTATGAAGAGACAAGCAGCTCCTGCTGCTGCTTGGGTAC CACGGACACCTGCTCTGGCCAGGAACAGCACGAGCAGGTGTGCGGATGAAGGTCAT |
| mm4_intron_Pex1_chr5_3609522 |
CAAGCGTCCTTGCTGCATTTCCTTACCTTTGACTGGGAAGAGAGGCTCTCCGTGGCTGCTGACCGCATGCCCCCGGCCAC AGCGAGGCCACATACGCTCTACTTTGTTTGGTATTTGTTTGGCTGGGCAGACAGAAA |
| mm4_intron_Mvb12b_chr2_33778293 |
TTTCTACCTGTGCCATGCCCTGGCTCCACGCTACCCCCTGAAGCCTCTCTGGCCAAATCAATTCTTTCAGCAGGATTGGC CAGGGCTGTGACGAGTGTACGGCAGCCCAGGTGCTCATGGAAGAGGGCCATT |
| mm4_intron_Maff_chr15_79352751 |
GGAGTTTCAGTCCTGGGCTCTGCCCTGTGTGGCTGTGTCCCCTCGGTCACCCTCTCTGGGCACATTACTTGGCAGTGTTA TCAGAAAGACTATGAAGATGATGGTAGGTCAAGAGTTAGTCCTG |
| mm4_intron_Ctnnd2_chr15_30879341 |
CCTGTGTTGTACTGCATCCCTTTCACTAATTCACACCTCGGAGAACCTCTCTGGGCAGTGAGGATATGAGACCCTTTATC TTTAGAAGAGTTTGGGGCCAGCAGAAGAAA |
| mm4_intron_Cntfr_chr4_41672424 |
CAGGACTGCTTGTCTCTGGGCTGGAGCATCTGTCTAGTTTGCTGCCTCTGTCACCCTCCCTGGCCAAGGTCTCTGTGTCT GCCTCCCTCTTG |
| mm4_intergenic_Pole4|Hk2_chr6_82718574 | ACCAGAGGGCATGAGAGATGAGAAAGCCCCAGAAAGCCCCTCTGGCCACTGACCCAGTCAAGGAGGAAGC |
| mm4_intergenic_Mfsd4/Gm26892|Gm26034_chr1_132098428 |
TGGCACAGAGGACCAAATGTTTCTCCTGGGTCACCCTCTCTGGGCAAAGGTCCTGTCTGAAACCTAGCAGCAGTCTGGGG TTCTCATGTTCTCTGACCAGGATGGGCTTTCCGGCTGAAGTCTGGAAAC |
| mm4_intergenic_Fat4|Gm9845_chr3_39173398 |
TCCATGTTGGTAGACAAGCATTTGGCCAGAGAATGTGTCTGGGGCTGCAAAGGTATTTTTACATTAGAAAGTAAAAGCTT GGGTGTACCATAGGTGTGTTCTTAATACGATTTGGAAACTGCCAACATGGACTCTAGCC |
| mm4_intergenic_2010007H06Rik|1810046K07Rik_chr9_51283137 |
GCTTTCCACCCAAGCCTTTGCCCCTGAAAGCCTCTCTGTCCATAGTCTTTCCCATCCTTCCTAAACCAGCTTTTCAAATC TCTGCTTTTCCATGCCACA |
| mm4_exon_Agbl4_chr4_111618974 |
GCGCATGGCTTCCCAAAATCTAGCCATTCTTAAATGCCCCTGGACACCCTCTCCTGCCAACATTTGGTAGTGTGATCCTC CAACTCCTTAAAGACACAGGTGAAGATCAACTCCACACCACACC |
| mm4_exon_1110008J03Rik_chr5_120611449 |
CTTGGTGTGAGGTTTGGGGTGCTGCCCCTGGAAGAGGGGGACTCACAGTCTCTCTTGGCCAGAGAGCCCTTCGGTGGTCC TGGGCCCACTCCTCTGGATCGGTTACAATTCTGCACCCAGGGTGGGTCAAAGTCTGGTT |