CRISPOR

DNA break repair outcome predictions

Guide: GCCAAGATCCCAGGGAGCCT GGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

mh- Score	Sequence	Effect
	`CGCCTTAGCTCCTCTTCTCAGCCAAGATCCCAGGGAGCCTGGGATTAGGAGCTTACTTGGGGGTTTTCCCCCTCCCCCCC`	(Wild-type sequence)
469	`CCTCTTCTCAGCCAAGATCCCAGGGA--------TTAGGAGCTTACTTGGGGGTTTTCCC`	`8 bp deleted → frameshift`
447	`CCTCTTCTCAGCCAAGATCCCAGGGAGC--------------TTACTTGGGGGTTTTCCC`	`14 bp deleted → frameshift`
282	`CCTCTTCTCAGCCAAGATCCCAGGG-------GATTAGGAGCTTACTTGGGGGTTTTCCC`	`7 bp deleted → frameshift`
255	`CCTCTTCTCAGCCAAGATCCCAGG---------ATTAGGAGCTTACTTGGGGGTTTTCCC`	`9 bp deleted → no frameshift`
235	`CCTCTTCTCAGCCAAGATCCCAGG---------------AGCTTACTTGGGGGTTTTCCC`	`15 bp deleted → no frameshift`
173	`CCTCTTCTCAGCCAAGATCCCAGGGAG-----------GAGCTTACTTGGGGGTTTTCCC`	`11 bp deleted → frameshift`
170	`CCTCTTCTCAGCCAAGAT-----------------TAGGAGCTTACTTGGGGGTTTTCCC`	`17 bp deleted → frameshift`
163	`CCTCTTCTCAGCCAAGATCCCAGGG--------------------------GGTTTTCCC`	`26 bp deleted → frameshift`
156	`CCTCTTCTCAGCCAAGATCCCAGGGAGCCT-------------TACTTGGGGGTTTTCCC`	`13 bp deleted → frameshift`
155	`CCTCTTCTCAGCCAAGATCCCAGGG---------------------------GTTTTCCC`	`27 bp deleted → no frameshift`
148	`CCTCTTCTCAGCCAAGATCCCAGGG----------------------------TTTTCCC`	`28 bp deleted → frameshift`
128	`CCTCTTCTCAGCCAAGATCCCAGGGAGCCT-----------------TGGGGGTTTTCCC`	`17 bp deleted → frameshift`
122	`CCTCTTCTCAGCCAAGATCCCAG------------------CTTACTTGGGGGTTTTCCC`	`18 bp deleted → no frameshift`
114	`CCTCTTCTCAGCCAAGATCCCAGGG-------------------------GGGTTTTCCC`	`25 bp deleted → frameshift`
111	`CCTCTTCTCAGC------------------------------TTACTTGGGGGTTTTCCC`	`30 bp deleted → no frameshift`
99	`CCTCTTCTCAGCCAAG----------------------GAGCTTACTTGGGGGTTTTCCC`	`22 bp deleted → frameshift`
99	`CCTCTTCTCAGCCAAGATCCC---------------------------------------`	`39 bp deleted → no frameshift`
95	`CCTCTTCTCAGCCAAGA-----------------------GCTTACTTGGGGGTTTTCCC`	`23 bp deleted → frameshift`
94	`CCTCTTCTCAGCCAAGATCCCAGG-----------------------------TTTTCCC`	`29 bp deleted → frameshift`
89	`CCTCTTCTCAGCCAAGATCCCAGGGAGCC------------------------------C`	`30 bp deleted → no frameshift`
86	`CCTCTTCTCAGCCAAG-------------------------CTTACTTGGGGGTTTTCCC`	`25 bp deleted → frameshift`
84	`CCTCTTCTCAGCCAAGATCCCAGGGAGCC-------------------------------`	`31 bp deleted → frameshift`
77	`CCTCTTCTCAG---------------------------GAGCTTACTTGGGGGTTTTCCC`	`27 bp deleted → no frameshift`
60	`CCTCTTCTCAGCCAAGATCCC--------------------------------------C`	`38 bp deleted → frameshift`
60	`CCTCTT--------------------------------------ACTTGGGGGTTTTCCC`	`38 bp deleted → frameshift`
54	`CCTCTTCTCAGCCAAGATCC----------------------------------------`	`40 bp deleted → frameshift`
52	`CCTCTTCT-----------------------------------TACTTGGGGGTTTTCCC`	`35 bp deleted → frameshift`
48	`CCTCTT------------------------------------------GGGGGTTTTCCC`	`42 bp deleted → no frameshift`
44	`CCTCTT------------------------------AGGAGCTTACTTGGGGGTTTTCCC`	`30 bp deleted → no frameshift`
42	`CCTCTTCT---------------------------------------TGGGGGTTTTCCC`	`39 bp deleted → no frameshift`
40	`CCT----------------------------------------TACTTGGGGGTTTTCCC`	`40 bp deleted → frameshift`
40	`CCTCTTCTCAGCC----------------------------------------------C`	`46 bp deleted → frameshift`
38	`CCTCTTCTCAGCC-----------------------------------------------`	`47 bp deleted → frameshift`
33	`CCT--------------------------------------------TGGGGGTTTTCCC`	`44 bp deleted → frameshift`
31	`CCTCTTC---------------------------------------------------CC`	`51 bp deleted → no frameshift`
25	`CCTCTTCTC-------------------------------------------------CC`	`49 bp deleted → frameshift`
23	`CC---------------------------------------------------------C`	`57 bp deleted → no frameshift`
22	`CC----------------------------------------------------------`	`58 bp deleted → frameshift`
20	`CCTC------------------------------------------------------CC`	`54 bp deleted → no frameshift`
17	`CCTCTT-------------------------------------------------TTCCC`	`49 bp deleted → frameshift`
16	`CCTCTT--------------------------------------------------TCCC`	`50 bp deleted → frameshift`

Version 5.01 - Documentation - Contact us - Downloads/local installation - Citation - License