CRISPOR

DNA break repair outcome predictions

Guide: TGGGGGTCCCGGGCCAAGCA TGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

mh- Score	Sequence	Effect
	`AGGCTGTCCGCGGAGGGGACTGGGGGTCCCGGGCCAAGCATGGCCGCTCCAGCAGACCGTGGCTCGGCGCCCAGCCCTGC`	(Wild-type sequence)
485	`CGGAGGGGACTGGGGGTCCCGGGCC----------GCTCCAGCAGACCGTGGCTCGGCGC`	`10 bp deleted → frameshift`
311	`CGGAGGGGACTGGGGGTCCCGGGCCAAGC-----CGCTCCAGCAGACCGTGGCTCGGCGC`	`5 bp deleted → frameshift`
298	`CGGAGGGGACTGGGGGTCCCGGGCCAAGCA--------------GACCGTGGCTCGGCGC`	`14 bp deleted → frameshift`
283	`CGGAGGGGACTGGGGGTCCCG---------------CTCCAGCAGACCGTGGCTCGGCGC`	`15 bp deleted → no frameshift`
268	`CGGAGGGGACTGGGGGTCCCGGGCCAAGC--------TCCAGCAGACCGTGGCTCGGCGC`	`8 bp deleted → frameshift`
235	`CGGAGGGGACTGGGGGTCCCGGGCCA---------------GCAGACCGTGGCTCGGCGC`	`15 bp deleted → no frameshift`
230	`CGGAGGGGACTGGGGGTCCCGG-----------CCGCTCCAGCAGACCGTGGCTCGGCGC`	`11 bp deleted → frameshift`
208	`CGGAGGGGACTGGGGGTCCCGGGC-------------TCCAGCAGACCGTGGCTCGGCGC`	`13 bp deleted → frameshift`
184	`CGGAGGGGACTGG--------------------CCGCTCCAGCAGACCGTGGCTCGGCGC`	`20 bp deleted → frameshift`
179	`CGGAGGGGACTGGGGGTCC----------------GCTCCAGCAGACCGTGGCTCGGCGC`	`16 bp deleted → frameshift`
175	`CGGAGGGGACTGGGGGTCC---------------------AGCAGACCGTGGCTCGGCGC`	`21 bp deleted → no frameshift`
173	`CGGAGGGGACTGGGGGTCCCGGGCCAAGCA-----------GCAGACCGTGGCTCGGCGC`	`11 bp deleted → frameshift`
170	`CGGAGGGGACTGGGGG-----------------CCGCTCCAGCAGACCGTGGCTCGGCGC`	`17 bp deleted → frameshift`
162	`CGGAGGGGACTGGGG------------------CCGCTCCAGCAGACCGTGGCTCGGCGC`	`18 bp deleted → no frameshift`
154	`CGGAGGGGACTGGGGGTCCCGGGC-------------------AGACCGTGGCTCGGCGC`	`19 bp deleted → frameshift`
154	`CGGAGGGGACTGGG-------------------CCGCTCCAGCAGACCGTGGCTCGGCGC`	`19 bp deleted → frameshift`
148	`CGGAGGGGACTGGGGGTCCCG----------------------------TGGCTCGGCGC`	`28 bp deleted → frameshift`
147	`CGGAGGGGACTGGGGGTCCC--------------------AGCAGACCGTGGCTCGGCGC`	`20 bp deleted → frameshift`
141	`CGGAGGGGACTGGGGGTCCCGGGC-----------------------------TCGGCGC`	`29 bp deleted → frameshift`
128	`CGGAGGGGACTGGGGGTCCCGGGCCAAG-----------------ACCGTGGCTCGGCGC`	`17 bp deleted → frameshift`
126	`CGGAGGGGACTGGGGGTCCCGGGCC-----------------------GTGGCTCGGCGC`	`23 bp deleted → frameshift`
122	`CGGAGGGGACTGGGGGTCCCGGGCCA------------------GACCGTGGCTCGGCGC`	`18 bp deleted → no frameshift`
120	`CGGAGGGGACTGGGGGTCCCGGGCCAAGC------------------------TCGGCGC`	`24 bp deleted → no frameshift`
114	`CGGAGGGG-------------------------CCGCTCCAGCAGACCGTGGCTCGGCGC`	`25 bp deleted → frameshift`
109	`CGGAGGGGACTGGGGGTCCCGGGC----------------------------------GC`	`34 bp deleted → frameshift`
109	`CGGAGGG--------------------------CCGCTCCAGCAGACCGTGGCTCGGCGC`	`26 bp deleted → frameshift`
104	`CGGAGGGGACTGGGGGTCCCGG-----------------------------------CGC`	`35 bp deleted → frameshift`
103	`CGGAGG---------------------------CCGCTCCAGCAGACCGTGGCTCGGCGC`	`27 bp deleted → no frameshift`
94	`CGGAGGGGACTGGGGGTCCCGGGCCAAGC-----------------------------GC`	`29 bp deleted → frameshift`
94	`CGGAGGGGACTGGGGGTCC-----------------------------GTGGCTCGGCGC`	`29 bp deleted → frameshift`
89	`CGGAGGGGACTGGGGGTCCCGG------------------------------CTCGGCGC`	`30 bp deleted → no frameshift`
89	`CGG------------------------------CCGCTCCAGCAGACCGTGGCTCGGCGC`	`30 bp deleted → no frameshift`
84	`CGGAGGGGACTGGGGGTCCCGGGCCAAGC-------------------------------`	`31 bp deleted → frameshift`
78	`CGGAGGGGAC-------------------------------------CGTGGCTCGGCGC`	`37 bp deleted → frameshift`
77	`CGGAGGGGACT---------------------------CCAGCAGACCGTGGCTCGGCGC`	`27 bp deleted → no frameshift`
73	`CG----------------------------------CTCCAGCAGACCGTGGCTCGGCGC`	`34 bp deleted → frameshift`
71	`CGGAGGGGACTGG---------------------------------------CTCGGCGC`	`39 bp deleted → no frameshift`
66	`CGGAGGGGACTGGGGGTCCCGGGC------------------------------------`	`36 bp deleted → no frameshift`
66	`CGGAGGGGACTGGGGG------------------------------------CTCGGCGC`	`36 bp deleted → no frameshift`
62	`CGGAGGGGACTGGGG-------------------------------------CTCGGCGC`	`37 bp deleted → frameshift`
60	`CGGAGGGGACTGGGGGTCCCG--------------------------------------C`	`38 bp deleted → frameshift`
60	`CGGAGGGGACTGGG--------------------------------------CTCGGCGC`	`38 bp deleted → frameshift`
57	`CGGAGGGGACTGGGGGT---------------------------------GGCTCGGCGC`	`33 bp deleted → no frameshift`
51	`CGGAGGGGACTGGGGG-----------------------------------------CGC`	`41 bp deleted → frameshift`
48	`CGGAGGGGACTGGGG------------------------------------------CGC`	`42 bp deleted → no frameshift`
47	`CGGAGGGGACTGGGGGTC-------------------------------------GGCGC`	`37 bp deleted → frameshift`
47	`CGGAG-------------------------------------CAGACCGTGGCTCGGCGC`	`37 bp deleted → frameshift`
46	`CGGAGGGGACTGGG-------------------------------------------CGC`	`43 bp deleted → frameshift`
44	`CGGAGGGGACTGG--------------------------------------------CGC`	`44 bp deleted → frameshift`
44	`CGGAGGGG--------------------------------------------CTCGGCGC`	`44 bp deleted → frameshift`
42	`CGGAGGG---------------------------------------------CTCGGCGC`	`45 bp deleted → no frameshift`
40	`CGGAG----------------------------------------ACCGTGGCTCGGCGC`	`40 bp deleted → frameshift`
40	`CGG------------------------------------------------------CGC`	`54 bp deleted → no frameshift`
40	`CGGAGG----------------------------------------------CTCGGCGC`	`46 bp deleted → frameshift`
38	`CG-----------------------------------------------TGGCTCGGCGC`	`47 bp deleted → frameshift`
36	`CGGA------------------------------------------CCGTGGCTCGGCGC`	`42 bp deleted → no frameshift`
34	`CGGAGGGGACT-------------------------------------------CGGCGC`	`43 bp deleted → frameshift`
34	`CGGAGGGG-------------------------------------------------CGC`	`49 bp deleted → frameshift`
34	`CGG-------------------------------------------------CTCGGCGC`	`49 bp deleted → frameshift`
32	`CGGAGGG--------------------------------------------------CGC`	`50 bp deleted → frameshift`
31	`CGGAGG---------------------------------------------------CGC`	`51 bp deleted → no frameshift`
23	`CG---------------------------------------------------------C`	`57 bp deleted → no frameshift`

Version 5.01 - Documentation - Contact us - Downloads/local installation - Citation - License