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Contents:In the table below, Illumina Nextera Handle sequences have been added and highlighted in bold. Primers for the on-target have been added for convenience. The table below is sorted by the CFD off-target score. Sites with very low CFD scores < 0.02 are unlikely to be cleaved, see our study Haeussler et al. 2016, Figure 2.
In the protocol by Matthew Canver, Harvard, two PCRs are run: one PCR to amplify the potential off-target, then a second PCR to extend the handles with Illumina barcodes. Please click here to download the protocol. Alternatively, you can have a look at Fu et al, 2014.
If a primer was not found, the reason is usually that the region around the off-target is too repetitive. To avoid unspecific primers, all repeats are masked for the primer design (not for off-target search). If you think that we should change the parameters here or should use different primer3 settings, please let us know.
| Name | Primer Sequence | Tm | CFD Score |
|---|---|---|---|
| ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52313651_F | TCGTCGGCAGCGTCTTGCAGCAGGAGGATGACAG | 60.0 | 1.00 |
| ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52313651_R | GTCTCGTGGGCTCGGAGATGGCAGTGGGGAGCTA | 59.9 | 1.00 |
| mm4_intron_Cachd1_chr4_100958659_F | Primer3: not found at this Tm | N.d. | 0.51 |
| mm4_intron_Cachd1_chr4_100958659_R | Primer3: not found at this Tm | N.d. | 0.51 |
| mm4_intergenic_Gm6207/Gm15020|Gm15020_chrX_135142485_F | Primer3: not found at this Tm | N.d. | 0.42 |
| mm4_intergenic_Gm6207/Gm15020|Gm15020_chrX_135142485_R | Primer3: not found at this Tm | N.d. | 0.42 |
| mm4_intergenic_Gm15017|Gm15017/Gm6215_chrX_135339870_F | Primer3: not found at this Tm | N.d. | 0.42 |
| mm4_intergenic_Gm15017|Gm15017/Gm6215_chrX_135339870_R | Primer3: not found at this Tm | N.d. | 0.42 |
| mm4_intergenic_Gm15015|Gm15015/Gm6228_chrX_135529680_F | Primer3: not found at this Tm | N.d. | 0.42 |
| mm4_intergenic_Gm15015|Gm15015/Gm6228_chrX_135529680_R | Primer3: not found at this Tm | N.d. | 0.42 |
| mm4_exon_4930453O03Rik/Trpm8_chr1_88348005_F | Primer3: not found at this Tm | N.d. | 0.29 |
| mm4_exon_4930453O03Rik/Trpm8_chr1_88348005_R | Primer3: not found at this Tm | N.d. | 0.29 |
| mm4_intergenic_Gm12016|Etaa1_chr11_17908471_F | Primer3: not found at this Tm | N.d. | 0.28 |
| mm4_intergenic_Gm12016|Etaa1_chr11_17908471_R | Primer3: not found at this Tm | N.d. | 0.28 |
| mm4_intergenic_Tacc1|Gm10689_chr8_25229669_F | Primer3: not found at this Tm | N.d. | 0.26 |
| mm4_intergenic_Tacc1|Gm10689_chr8_25229669_R | Primer3: not found at this Tm | N.d. | 0.26 |
| mm4_intron_Slc51a_chr16_32477474_F | Primer3: not found at this Tm | N.d. | 0.26 |
| mm4_intron_Slc51a_chr16_32477474_R | Primer3: not found at this Tm | N.d. | 0.26 |
| mm3_intron_Capzb_chr4_139243063_F | Primer3: not found at this Tm | N.d. | 0.26 |
| mm3_intron_Capzb_chr4_139243063_R | Primer3: not found at this Tm | N.d. | 0.26 |
| mm4_intron_Enox2_chrX_49251792_F | Primer3: not found at this Tm | N.d. | 0.25 |
| mm4_intron_Enox2_chrX_49251792_R | Primer3: not found at this Tm | N.d. | 0.25 |
| mm4_intergenic_n-R5s63|Rgs6_chr12_82587917_F | Primer3: not found at this Tm | N.d. | 0.24 |
| mm4_intergenic_n-R5s63|Rgs6_chr12_82587917_R | Primer3: not found at this Tm | N.d. | 0.24 |
| mm4_intron_Inpp5a_chr7_139474444_F | TCGTCGGCAGCGTCTTAATCATCTATGCTTCCACCCA | 57.3 | 0.24 |
| mm4_intron_Inpp5a_chr7_139474444_R | GTCTCGTGGGCTCGGCCCCTCCCTCGTCTATAGGA | 58.9 | 0.24 |
| mm4_intergenic_Poglut1|Tmem39a_chr16_38555274_F | Primer3: not found at this Tm | N.d. | 0.23 |
| mm4_intergenic_Poglut1|Tmem39a_chr16_38555274_R | Primer3: not found at this Tm | N.d. | 0.23 |
| mm4_intergenic_Lcn5|Lcn6_chr2_25669314_F | Primer3: not found at this Tm | N.d. | 0.23 |
| mm4_intergenic_Lcn5|Lcn6_chr2_25669314_R | Primer3: not found at this Tm | N.d. | 0.23 |
| mm4_intergenic_Gnai1|Gm17037_chr5_18380497_F | Primer3: not found at this Tm | N.d. | 0.23 |
| mm4_intergenic_Gnai1|Gm17037_chr5_18380497_R | Primer3: not found at this Tm | N.d. | 0.23 |
| mm4_intergenic_Adamts5|Gm25715_chr16_86378677_F | Primer3: not found at this Tm | N.d. | 0.22 |
| mm4_intergenic_Adamts5|Gm25715_chr16_86378677_R | Primer3: not found at this Tm | N.d. | 0.22 |
| mm4_intron_Rgs20_chr1_4911113_F | Primer3: not found at this Tm | N.d. | 0.20 |
| mm4_intron_Rgs20_chr1_4911113_R | Primer3: not found at this Tm | N.d. | 0.20 |
| mm4_intron_Ldlrad3_chr2_102119758_F | TCGTCGGCAGCGTCGAGCCTTTGTGCTCTGGACT | 59.9 | 0.19 |
| mm4_intron_Ldlrad3_chr2_102119758_R | GTCTCGTGGGCTCGGTGCTCCCCTATCCAAGCTTG | 59.4 | 0.19 |
| mm4_intron_Plcb4_chr2_135789157_F | TCGTCGGCAGCGTCGACCAGAGTGCCTTCCGAAT | 59.7 | 0.19 |
| mm4_intron_Plcb4_chr2_135789157_R | GTCTCGTGGGCTCGGTGCAAAGCAGGTGCATATGC | 59.8 | 0.19 |
| mm4_exon_Rilpl1_chr5_124530673_F | TCGTCGGCAGCGTCTCCACAAAGGACACTAGCGG | 59.6 | 0.19 |
| mm4_exon_Rilpl1_chr5_124530673_R | GTCTCGTGGGCTCGGGAGAGGATGGACCGCATTGA | 59.5 | 0.19 |
| mm4_intron_Lsamp_chr16_42036579_F | Primer3: not found at this Tm | N.d. | 0.17 |
| mm4_intron_Lsamp_chr16_42036579_R | Primer3: not found at this Tm | N.d. | 0.17 |
| mm4_intergenic_Btd|Ankrd28_chr14_31690247_F | Primer3: not found at this Tm | N.d. | 0.17 |
| mm4_intergenic_Btd|Ankrd28_chr14_31690247_R | Primer3: not found at this Tm | N.d. | 0.17 |
| mm4_intergenic_Gm20682|3300002A11Rik_chr12_99340090_F | Primer3: not found at this Tm | N.d. | 0.17 |
| mm4_intergenic_Gm20682|3300002A11Rik_chr12_99340090_R | Primer3: not found at this Tm | N.d. | 0.17 |
| mm4_intergenic_Gm24092|Cenpf_chr1_189457581_F | Primer3: not found at this Tm | N.d. | 0.16 |
| mm4_intergenic_Gm24092|Cenpf_chr1_189457581_R | Primer3: not found at this Tm | N.d. | 0.16 |
| mm4_intergenic_Gm12339|Rph3al_chr11_75829157_F | Primer3: not found at this Tm | N.d. | 0.15 |
| mm4_intergenic_Gm12339|Rph3al_chr11_75829157_R | Primer3: not found at this Tm | N.d. | 0.15 |
| mm4_intergenic_Cbln2|Gm5096_chr18_87246056_F | TCGTCGGCAGCGTCGCTGCCTTTGCCTCAGGATA | 60.1 | 0.15 |
| mm4_intergenic_Cbln2|Gm5096_chr18_87246056_R | GTCTCGTGGGCTCGGGGATTACCTGTCCAGCTGCA | 59.7 | 0.15 |
| mm4_intergenic_Gm23452|Gm23900_chr2_81238295_F | Primer3: not found at this Tm | N.d. | 0.14 |
| mm4_intergenic_Gm23452|Gm23900_chr2_81238295_R | Primer3: not found at this Tm | N.d. | 0.14 |
| mm4_exon_Nkpd1_chr7_19524369_F | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_exon_Nkpd1_chr7_19524369_R | Primer3: not found at this Tm | N.d. | 0.13 |
| mm3_exon_B3gat1_chr9_26734068_F | Primer3: not found at this Tm | N.d. | 0.13 |
| mm3_exon_B3gat1_chr9_26734068_R | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_intron_Nbas_chr12_13474308_F | TCGTCGGCAGCGTCCCATGCCTGTCTCTGCAAGA | 60.0 | 0.13 |
| mm4_intron_Nbas_chr12_13474308_R | GTCTCGTGGGCTCGGAAGGAAAGCCTGTGCCAAGA | 59.8 | 0.13 |
| mm4_exon_Tram2_chr1_20997458_F | Primer3: not found at this Tm | N.d. | 0.12 |
| mm4_exon_Tram2_chr1_20997458_R | Primer3: not found at this Tm | N.d. | 0.12 |
| mm4_intron_Timp3_chr10_86310638_F | Primer3: not found at this Tm | N.d. | 0.11 |
| mm4_intron_Timp3_chr10_86310638_R | Primer3: not found at this Tm | N.d. | 0.11 |
| mm4_intron_Zdhhc3_chr9_123080911_F | Primer3: not found at this Tm | N.d. | 0.11 |
| mm4_intron_Zdhhc3_chr9_123080911_R | Primer3: not found at this Tm | N.d. | 0.11 |
| mm4_intron_Bmp7_chr2_172871979_F | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intron_Bmp7_chr2_172871979_R | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intron_Ajap1_chr4_153434367_F | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intron_Ajap1_chr4_153434367_R | Primer3: not found at this Tm | N.d. | 0.10 |
| mm3_intergenic_n-R5s153|Catsperg2_chr7_29692685_F | Primer3: not found at this Tm | N.d. | 0.10 |
| mm3_intergenic_n-R5s153|Catsperg2_chr7_29692685_R | Primer3: not found at this Tm | N.d. | 0.10 |
| mm3_intergenic_Zfp260|Zfp382_chr7_30112886_F | Primer3: not found at this Tm | N.d. | 0.10 |
| mm3_intergenic_Zfp260|Zfp382_chr7_30112886_R | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intron_Cpne4_chr9_104556434_F | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intron_Cpne4_chr9_104556434_R | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intron_Cdkal1_chr13_29368881_F | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intron_Cdkal1_chr13_29368881_R | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intron_Oca2_chr7_56463407_F | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intron_Oca2_chr7_56463407_R | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intergenic_Rasa2|Zbtb38_chr9_96664470_F | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intergenic_Rasa2|Zbtb38_chr9_96664470_R | Primer3: not found at this Tm | N.d. | 0.09 |
| mm3_intergenic_AC121988.1|Gm25740_chr10_46340325_F | Primer3: not found at this Tm | N.d. | 0.09 |
| mm3_intergenic_AC121988.1|Gm25740_chr10_46340325_R | Primer3: not found at this Tm | N.d. | 0.09 |
| mm3_intron_Jup_chr11_100396037_F | TCGTCGGCAGCGTCAGGCCACTCTTCGGATTTGT | 59.3 | 0.09 |
| mm3_intron_Jup_chr11_100396037_R | GTCTCGTGGGCTCGGTGAGGCTGCACAGTGTGATC | 60.3 | 0.09 |
| mm3_intron_Grid2_chr6_64309665_F | Primer3: not found at this Tm | N.d. | 0.08 |
| mm3_intron_Grid2_chr6_64309665_R | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intron_Fam222b_chr11_78133859_F | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intron_Fam222b_chr11_78133859_R | Primer3: not found at this Tm | N.d. | 0.08 |
| mm3_intergenic_Zfp930|D130040H23Rik_chr8_69248180_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm3_intergenic_Zfp930|D130040H23Rik_chr8_69248180_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intron_Myo5b_chr18_74762442_F | TCGTCGGCAGCGTCCAAGCCTAGGGAAAGGGTGG | 60.0 | 0.07 |
| mm4_intron_Myo5b_chr18_74762442_R | GTCTCGTGGGCTCGGAGACTCTGGACAGACCCCTC | 59.9 | 0.07 |
| mm4_intergenic_Gm14768|Gm5940_chrX_80418652_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_Gm14768|Gm5940_chrX_80418652_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_Igsf10|Gm5709_chr3_59469666_F | TCGTCGGCAGCGTCTGATTGAGAACTGTGGGATGGA | 59.0 | 0.07 |
| mm4_intergenic_Igsf10|Gm5709_chr3_59469666_R | GTCTCGTGGGCTCGGAAGGTCAGAGATGGTGCACC | 59.6 | 0.07 |
| mm4_intron_Lat_chr7_126367821_F | TCGTCGGCAGCGTCGAAATGGAAGGGCGGCAATC | 59.8 | 0.07 |
| mm4_intron_Lat_chr7_126367821_R | GTCTCGTGGGCTCGGCCTAGCAACCCTGACCTTGG | 60.0 | 0.07 |
| mm4_intron_Cntnap2_chr6_47064319_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intron_Cntnap2_chr6_47064319_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_Zfp623|Zfp707_chr15_75962377_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_Zfp623|Zfp707_chr15_75962377_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_Vmn2r-ps2|Gm13506_chr2_39303003_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_Vmn2r-ps2|Gm13506_chr2_39303003_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_Mettl21c|Gm8251_chr1_44046061_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_Mettl21c|Gm8251_chr1_44046061_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_Map7d2|A830080D01Rik_chrX_159513342_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_Map7d2|A830080D01Rik_chrX_159513342_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_Mageb16|Gm4772_chrX_79771400_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_Mageb16|Gm4772_chrX_79771400_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_Large|Gm23255_chr8_73382519_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_Large|Gm23255_chr8_73382519_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_Hspe1|Gm16581_chr1_55104725_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_Hspe1|Gm16581_chr1_55104725_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_Hint3|Ncoa7_chr10_30634801_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_Hint3|Ncoa7_chr10_30634801_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_Gm8325|P2ry1_chr3_60939218_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_Gm8325|P2ry1_chr3_60939218_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_Gm26235|Gm25392_chr18_18291260_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_Gm26235|Gm25392_chr18_18291260_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_Gm24863|Gm24635_chr13_79510677_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_Gm24863|Gm24635_chr13_79510677_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_Gm24694|Gm25863_chr3_16102138_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_Gm24694|Gm25863_chr3_16102138_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_Gm23893|Gm10181_chr9_25018472_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_Gm23893|Gm10181_chr9_25018472_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_Gm23021|8430419K02Rik_chr11_18834433_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_Gm23021|8430419K02Rik_chr11_18834433_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_Gm12080|Gm6685_chr11_28061207_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_Gm12080|Gm6685_chr11_28061207_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_Ddx6|Treh_chr9_44669098_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_Ddx6|Treh_chr9_44669098_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_Cox6c|Rgs22_chr15_35957940_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_Cox6c|Rgs22_chr15_35957940_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_Arl14epl|Commd10_chr18_46942434_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_Arl14epl|Commd10_chr18_46942434_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_Acox2|Fam107a_chr14_8277103_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_Acox2|Fam107a_chr14_8277103_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_4922501C03Rik|Tbx18_chr9_87287468_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_4922501C03Rik|Tbx18_chr9_87287468_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intron_Tmem132c_chr5_127425333_F | TCGTCGGCAGCGTCGGGGTGGGGCAGAGAATAAA | 59.3 | 0.06 |
| mm4_intron_Tmem132c_chr5_127425333_R | GTCTCGTGGGCTCGGTCTTACCCTTCTCCCAACCCA | 60.1 | 0.06 |
| mm4_intergenic_Rpp40|Ppp1r3g_chr13_35926184_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_Rpp40|Ppp1r3g_chr13_35926184_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_Gm26341|Amd2_chr10_35446514_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_Gm26341|Amd2_chr10_35446514_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_Gm22960|Kif13a_chr13_46908326_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Gm22960|Kif13a_chr13_46908326_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intron_Urah_chr7_140837310_F | TCGTCGGCAGCGTCCACACACTCACTACGGCAGT | 59.9 | 0.04 |
| mm4_intron_Urah_chr7_140837310_R | GTCTCGTGGGCTCGGACTTGGAGGAGTCCTAGGGG | 59.6 | 0.04 |
| mm4_intron_D630045J12Rik_chr6_38150248_F | TCGTCGGCAGCGTCTGTGTCAAAGACTGCTCTACTGT | 59.6 | 0.04 |
| mm4_intron_D630045J12Rik_chr6_38150248_R | GTCTCGTGGGCTCGGTTTTGTGGCTCTAGACCCGG | 59.6 | 0.04 |
| mm4_intergenic_Adamts17|1700112J16Rik_chr7_67162121_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Adamts17|1700112J16Rik_chr7_67162121_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intron_Epb4.1_chr4_131970796_F | TCGTCGGCAGCGTCAGCCAGACAAAAGTGACCCT | 59.1 | 0.03 |
| mm4_intron_Epb4.1_chr4_131970796_R | GTCTCGTGGGCTCGGTATTCTCTCCCTGGCTCCCC | 60.1 | 0.03 |
| mm4_intron_Alcam_chr16_52312097_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intron_Alcam_chr16_52312097_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Gm25038|D16Ertd472e_chr16_78460745_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Gm25038|D16Ertd472e_chr16_78460745_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Gm25200|Cdh13_chr8_118144481_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Gm25200|Cdh13_chr8_118144481_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intron_Ube2h_chr6_30260037_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intron_Ube2h_chr6_30260037_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Gata6|Rbbp8_chr18_11399700_F | TCGTCGGCAGCGTCTGCTCCATGTCTTTCCCAGA | 58.6 | 0.02 |
| mm4_intergenic_Gata6|Rbbp8_chr18_11399700_R | GTCTCGTGGGCTCGGTTGTCCTGCGTACGGTGATC | 60.1 | 0.02 |
| mm4_intergenic_A930002H24Rik|Fer_chr17_63881142_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_A930002H24Rik|Fer_chr17_63881142_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Gm12753|Gm12751_chr4_34211187_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Gm12753|Gm12751_chr4_34211187_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Gm9915|2610017I09Rik_chr1_42410296_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Gm9915|2610017I09Rik_chr1_42410296_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Gm9843|Usp25_chr16_76828208_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Gm9843|Usp25_chr16_76828208_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Schip1|Gm10292_chr3_68332753_F | TCGTCGGCAGCGTCGCACTGGACAGGTGGCTATT | 60.0 | 0.02 |
| mm4_intergenic_Schip1|Gm10292_chr3_68332753_R | GTCTCGTGGGCTCGGCTGCCCTTGGACTGTCCTTC | 60.3 | 0.02 |
| mm4_intergenic_Gm8793|Nkain2_chr10_31773177_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Gm8793|Nkain2_chr10_31773177_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_exon_Tceanc2/Gm12802_chr4_107134663_F | TCGTCGGCAGCGTCCTGTTGGACCTGGAGACCAA | 59.2 | 0.00 |
| mm4_exon_Tceanc2/Gm12802_chr4_107134663_R | GTCTCGTGGGCTCGGGGAACAGGGGACACAAGCTT | 60.1 | 0.00 |
| mm4_intergenic_Gm15387|Bai1_chr15_74210127_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm15387|Bai1_chr15_74210127_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Frmd4a|Gm2639_chr2_4035508_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Frmd4a|Gm2639_chr2_4035508_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Ctsll3|4930486L24Rik_chr13_60815537_F | TCGTCGGCAGCGTCCTGTTGCTCTGTTGCTCTCA | 58.1 | 0.00 |
| mm4_intergenic_Ctsll3|4930486L24Rik_chr13_60815537_R | GTCTCGTGGGCTCGGGTCTTCAGGTCCTGGTGTGG | 59.9 | 0.00 |
These only list off-targets that have primers in the table above. Primers underlined, off-targets in bold.
| ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52313651 |
TTGCAGCAGGAGGATGACAGCTTAGAAAGAAGAGGGCAATGGGGCTTCCTCCCAGAGGCGGTGCGGCACAGAGGAGCGCT CGCATCACAAGGTGACCCTAGCTCCCCACTGCCATCT |
| mm4_intron_Inpp5a_chr7_139474444 |
TTAATCATCTATGCTTCCACCCAAGGGGAACTCACGCATCACATGGTGGGTAGGTGTCATACAACTAAAAAATTCCTCCA ACCTAAGAAAGCTGTAGCCAAAGAAGCAAGAGAAGAGGTCCTATAGACGAGGGAGGGG |
| mm4_intron_Ldlrad3_chr2_102119758 |
GAGCCTTTGTGCTCTGGACTGTTCCCGTGTGATGTGAGCGCTACACAGAACCTCGGACATCTGTCCTGTGTATTTTTCCA AAAGCCATTTGGTCCTCATCATGCAAATATCTTCCTGTTCAAGCTTGGATAGGGGAGCA |
| mm4_intron_Plcb4_chr2_135789157 |
GACCAGAGTGCCTTCCGAATAGAATAGGAGGGCCAGCATCACAGGGGTGACCTCTAATTTGCAATTAAAATGACTGTTGA CTATGCATATGCACCTGCTTTGCA |
| mm4_exon_Rilpl1_chr5_124530673 |
TCCACAAAGGACACTAGCGGCTGCCCAGAACCGAAGGGCAGCCACGGAGCCCACGCCCCTTGCCCAGACCGGCAGACTGA GGCCTCCGCCCACCTTTTGATGCTTGCGCTCCTTTTCAATGCGGTCCATCCTCTC |
| mm4_intergenic_Cbln2|Gm5096_chr18_87246056 |
GCTGCCTTTGCCTCAGGATACCCAGCACTCATCAAAATCTCCCCCAGGTAATGCCAAGAGAGATGCTTCCTTTCAATGCC TTGTGATTTGTGTGCTCCTCTCTGCAGCTGGACAGGTAATCC |
| mm4_intron_Nbas_chr12_13474308 |
CCATGCCTGTCTCTGCAAGATCTAGTTTAGAACAGTCATGAAACCATGTAATGCCAGTGTTCCTCTTATCTATAGCCCAG GCTGTCTTGGCACAGGCTTTCCTT |
| mm3_intron_Jup_chr11_100396037 |
AGGCCACTCTTCGGATTTGTATAACCAGTGCAGCAAGGGCGAGTAGAAGTTCACAATTCCAGCCAGAGAAGCGCTCTCAG CACACGGAAGGCAAGGGGAGGGCGCGGATCACACTGTGCAGCCTCA |
| mm4_intron_Myo5b_chr18_74762442 |
CAAGCCTAGGGAAAGGGTGGGCATCGAGGACATGAGCATCAGACAGGAGCTCACGCCTCACAGGGTCGGCCATGGGCCTG ACCACACACCTTGAGGGGTCTGTCCAGAGTCT |
| mm4_intergenic_Igsf10|Gm5709_chr3_59469666 |
TGATTGAGAACTGTGGGATGGATTATTTTTTATATGTATATGTATATATATAAAGAGGGGCCCTTGCAGCACATGGGAAT TTGGTGCACCATCTCTGACCTT |
| mm4_intron_Lat_chr7_126367821 |
GAAATGGAAGGGCGGCAATCGTCACGGAGGTGAGAGGGAGGCAAGAGTGGAGAGCTGGCAGCACATGGCAGAGGGAGGTA GACAGAAAGCCTGACTCACCAGAGAAGGCACTGTCTCCAAGGTCAGGGTTGCTAGG |
| mm4_intron_Tmem132c_chr5_127425333 |
GGGGTGGGGCAGAGAATAAAACCTACCCATTGATGCCAGGGCTCCTCTAACCATGGCAGGTCTCATATCTTCAAGATGTC AGAGAGAAAGATTGAGCCATGGGTTGGGAGAAGGGTAAGA |
| mm4_intron_Urah_chr7_140837310 |
CACACACTCACTACGGCAGTGGGGACATATAAAAGAGGAGCGCTGTCACCTCATGGGGGACAAGCAGAAGAAATGAACAC AAGGGAAGACATGGTCCCCTAGGACTCCTCCAAGT |
| mm4_intron_D630045J12Rik_chr6_38150248 |
TGTGTCAAAGACTGCTCTACTGTTTTTACTACATGTAGTTAAAAATCTTCTTAAAGAAAAGGGAAGCTCATTTGAGAGGA GCGCTTCCATAGCATGGATCAGACCCCTGCTTCCGGGTCTAGAGCCACAAAA |
| mm4_intron_Epb4.1_chr4_131970796 |
AGCCAGACAAAAGTGACCCTCACATGAGCCCTCGCCTCACATGGTGGTGCCAGGAAGAGACCTGAGTTTCCTGTGGAATG TGAATGATGCCAACTGTAGGGGAGCCAGGGAGAGAATA |
| mm4_intergenic_Gata6|Rbbp8_chr18_11399700 | TGCTCCATGTCTTTCCCAGATCCCTTGTGAGTCCAGCTCTCCTCTGGAGCTTTCCAGCAGATCACCGTACGCAGGACAA |
| mm4_intergenic_Schip1|Gm10292_chr3_68332753 |
GCACTGGACAGGTGGCTATTCATCCTCATTAGGGCTGTCACACCCCTGTCGACAGAGAGGAGGGCTTTCTTCACATGGAA GGAATCTAAGCTGTTTGTCTCTACGTCCCTACCTTCTGAAGGACAGTCCAAGGGCAG |
| mm4_exon_Tceanc2/Gm12802_chr4_107134663 |
CTGTTGGACCTGGAGACCAATCTAGCCTATAGATAATCCTGACAGCAACTATATCGTTTGGGGGGAATTTAAAAGGAAAC ACCATGTCAAGCTAGCTCTCCTCTGAAGCTTGTGTCCCCTGTTCC |
| mm4_intergenic_Ctsll3|4930486L24Rik_chr13_60815537 |
CTGTTGCTCTGTTGCTCTCAAGCCGTGTGATCTGAGGCCTCCTCTTTTAGGCTGCACAAGCTCTTATTACCTCCACACCA GGACCTGAAGAC |