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Contents:In the table below, Illumina Nextera Handle sequences have been added and highlighted in bold. Primers for the on-target have been added for convenience. The table below is sorted by the CFD off-target score. Sites with very low CFD scores < 0.02 are unlikely to be cleaved, see our study Haeussler et al. 2016, Figure 2.
In the protocol by Matthew Canver, Harvard, two PCRs are run: one PCR to amplify the potential off-target, then a second PCR to extend the handles with Illumina barcodes. Please click here to download the protocol. Alternatively, you can have a look at Fu et al, 2014.
If a primer was not found, the reason is usually that the region around the off-target is too repetitive. To avoid unspecific primers, all repeats are masked for the primer design (not for off-target search). If you think that we should change the parameters here or should use different primer3 settings, please let us know.
| Name | Primer Sequence | Tm | CFD Score |
|---|---|---|---|
| ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52314194_F | Primer3: not found at this Tm | N.d. | 1.00 |
| ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52314194_R | Primer3: not found at this Tm | N.d. | 1.00 |
| mm4_intergenic_Gm13478|1700019E08Rik_chr2_45693238_F | TCGTCGGCAGCGTCACAAGGCAGGGAAGAAGTGG | 59.8 | 0.73 |
| mm4_intergenic_Gm13478|1700019E08Rik_chr2_45693238_R | GTCTCGTGGGCTCGGTCTGCTACAATATCCCTGTCTTTCT | 59.3 | 0.73 |
| mm4_intergenic_Dyrk1b|Eid2_chr7_28190611_F | Primer3: not found at this Tm | N.d. | 0.60 |
| mm4_intergenic_Dyrk1b|Eid2_chr7_28190611_R | Primer3: not found at this Tm | N.d. | 0.60 |
| mm3_intron_Clvs1_chr4_9348303_F | TCGTCGGCAGCGTCAAGAGGAAGGGAGTGTGGGG | 60.8 | 0.59 |
| mm3_intron_Clvs1_chr4_9348303_R | GTCTCGTGGGCTCGGGCTCATTGGTACGGAAGCCT | 60.1 | 0.59 |
| mm3_intron_Ankfn1_chr11_89510951_F | TCGTCGGCAGCGTCACACACGCATAAAGGTTGTCA | 58.4 | 0.57 |
| mm3_intron_Ankfn1_chr11_89510951_R | GTCTCGTGGGCTCGGAACTGCCTTTCTTGGTTGCC | 59.2 | 0.57 |
| mm3_intron_Nell1_chr7_50173617_F | Primer3: not found at this Tm | N.d. | 0.56 |
| mm3_intron_Nell1_chr7_50173617_R | Primer3: not found at this Tm | N.d. | 0.56 |
| mm4_intergenic_Gm27023|Gm25800_chr17_61987049_F | Primer3: not found at this Tm | N.d. | 0.48 |
| mm4_intergenic_Gm27023|Gm25800_chr17_61987049_R | Primer3: not found at this Tm | N.d. | 0.48 |
| mm3_intergenic_Ctbp2|Ctbp2/Fgfr2_chr7_133068819_F | Primer3: not found at this Tm | N.d. | 0.48 |
| mm3_intergenic_Ctbp2|Ctbp2/Fgfr2_chr7_133068819_R | Primer3: not found at this Tm | N.d. | 0.48 |
| mm4_intergenic_mmu-mir-6354|Gm5265_chr1_169253941_F | Primer3: not found at this Tm | N.d. | 0.46 |
| mm4_intergenic_mmu-mir-6354|Gm5265_chr1_169253941_R | Primer3: not found at this Tm | N.d. | 0.46 |
| mm4_intergenic_Gm10247|Grin2a_chr16_9575146_F | TCGTCGGCAGCGTCCCATTTGCCACTCCCTGGAT | 60.0 | 0.45 |
| mm4_intergenic_Gm10247|Grin2a_chr16_9575146_R | GTCTCGTGGGCTCGGCCCCTCCACTAATCCACTGAC | 59.5 | 0.45 |
| mm3_intergenic_Gm23083|Gm24773_chr16_80679807_F | TCGTCGGCAGCGTCACCTGAAGTTCATCTGTTAGCACA | 60.2 | 0.44 |
| mm3_intergenic_Gm23083|Gm24773_chr16_80679807_R | GTCTCGTGGGCTCGGAGCAATGAAATGAATGGGAACTCT | 58.9 | 0.44 |
| mm4_intergenic_Gpr158|Gm13337_chr2_21950841_F | Primer3: not found at this Tm | N.d. | 0.40 |
| mm4_intergenic_Gpr158|Gm13337_chr2_21950841_R | Primer3: not found at this Tm | N.d. | 0.40 |
| mm4_intergenic_Msr1|Fgf20_chr8_39899289_F | Primer3: not found at this Tm | N.d. | 0.38 |
| mm4_intergenic_Msr1|Fgf20_chr8_39899289_R | Primer3: not found at this Tm | N.d. | 0.38 |
| mm4_intron_Gm14569_chrX_36427936_F | TCGTCGGCAGCGTCACATGCTTTCTTGAGGTCCGT | 59.9 | 0.37 |
| mm4_intron_Gm14569_chrX_36427936_R | GTCTCGTGGGCTCGGTGAGGATCAGGGATGGTGGA | 59.6 | 0.37 |
| mm4_intron_Dock5_chr14_67892490_F | TCGTCGGCAGCGTCTGTGACCACCAAACTTATAAAACCA | 59.1 | 0.31 |
| mm4_intron_Dock5_chr14_67892490_R | GTCTCGTGGGCTCGGCCAGAAGACAAGCGAGCCAT | 60.3 | 0.31 |
| mm4_intergenic_Gm23896|Gm24902_chr5_57039957_F | Primer3: not found at this Tm | N.d. | 0.29 |
| mm4_intergenic_Gm23896|Gm24902_chr5_57039957_R | Primer3: not found at this Tm | N.d. | 0.29 |
| mm3_intergenic_Cpeb2|C1qtnf7_chr5_43450893_F | Primer3: not found at this Tm | N.d. | 0.29 |
| mm3_intergenic_Cpeb2|C1qtnf7_chr5_43450893_R | Primer3: not found at this Tm | N.d. | 0.29 |
| mm4_intergenic_Gm10650|Gm22293_chr3_128115965_F | Primer3: not found at this Tm | N.d. | 0.28 |
| mm4_intergenic_Gm10650|Gm22293_chr3_128115965_R | Primer3: not found at this Tm | N.d. | 0.28 |
| mm4_intergenic_Tbc1d8|Gm24826_chr1_39493623_F | Primer3: not found at this Tm | N.d. | 0.28 |
| mm4_intergenic_Tbc1d8|Gm24826_chr1_39493623_R | Primer3: not found at this Tm | N.d. | 0.28 |
| mm4_intergenic_Gm26651|Diras2_chr13_52128681_F | TCGTCGGCAGCGTCAGTTCTCACTGACAGCCGTG | 59.9 | 0.28 |
| mm4_intergenic_Gm26651|Diras2_chr13_52128681_R | GTCTCGTGGGCTCGGTTGCCACATTCACCTCCCAA | 59.8 | 0.28 |
| mm3_intergenic_Trim72|Gm22809_chr7_128011790_F | TCGTCGGCAGCGTCAGTCTCTCCCATGGCCTCTA | 59.0 | 0.28 |
| mm3_intergenic_Trim72|Gm22809_chr7_128011790_R | GTCTCGTGGGCTCGGCAGCAGGGCCCAAAGAAAGA | 60.5 | 0.28 |
| mm4_exon_Mov10_chr3_104795417_F | Primer3: not found at this Tm | N.d. | 0.27 |
| mm4_exon_Mov10_chr3_104795417_R | Primer3: not found at this Tm | N.d. | 0.27 |
| mm3_intergenic_Cdh7|Cdh19_chr1_110274873_F | TCGTCGGCAGCGTCACTCAAGTACCAATGCATCTGA | 57.4 | 0.27 |
| mm3_intergenic_Cdh7|Cdh19_chr1_110274873_R | GTCTCGTGGGCTCGGCCTGAGGCATGGTTATACAGT | 57.1 | 0.27 |
| mm4_intergenic_Gm4746|Gm6977_chrX_90455303_F | Primer3: not found at this Tm | N.d. | 0.27 |
| mm4_intergenic_Gm4746|Gm6977_chrX_90455303_R | Primer3: not found at this Tm | N.d. | 0.27 |
| mm3_intergenic_mmu-mir-7689|Sorcs2_chr5_36311543_F | TCGTCGGCAGCGTCGAGCCACACTCTGCTCACAG | 60.6 | 0.27 |
| mm3_intergenic_mmu-mir-7689|Sorcs2_chr5_36311543_R | GTCTCGTGGGCTCGGACCTTGCATCTGGGTTAGGC | 60.0 | 0.27 |
| mm4_intron_Cntnap5c_chr17_58165349_F | Primer3: not found at this Tm | N.d. | 0.26 |
| mm4_intron_Cntnap5c_chr17_58165349_R | Primer3: not found at this Tm | N.d. | 0.26 |
| mm4_intergenic_P2ry1|B430305J03Rik/Rap2b_chr3_61159283_F | Primer3: not found at this Tm | N.d. | 0.25 |
| mm4_intergenic_P2ry1|B430305J03Rik/Rap2b_chr3_61159283_R | Primer3: not found at this Tm | N.d. | 0.25 |
| mm4_intron_Mettl24_chr10_40790174_F | TCGTCGGCAGCGTCTCCAAACTGTAATAGATGGGGATCA | 59.3 | 0.25 |
| mm4_intron_Mettl24_chr10_40790174_R | GTCTCGTGGGCTCGGTTTAAAAGTTGTGCACAGCTGA | 57.2 | 0.25 |
| mm4_intergenic_Uncx|Micall2_chr5_139697920_F | TCGTCGGCAGCGTCGCTGCTTCTGTGACACCAATG | 60.0 | 0.24 |
| mm4_intergenic_Uncx|Micall2_chr5_139697920_R | GTCTCGTGGGCTCGGCGAGGGATACAAGGGGATGC | 59.9 | 0.24 |
| mm4_intergenic_Gm11680|BC006965_chr11_112598796_F | TCGTCGGCAGCGTCGGCTGGCTTTGCATCTAGGA | 60.1 | 0.24 |
| mm4_intergenic_Gm11680|BC006965_chr11_112598796_R | GTCTCGTGGGCTCGGGGTCCCTATGGATGGTCTGA | 57.8 | 0.24 |
| mm4_intergenic_Gm22636|Gm14970_chrX_127409196_F | Primer3: not found at this Tm | N.d. | 0.23 |
| mm4_intergenic_Gm22636|Gm14970_chrX_127409196_R | Primer3: not found at this Tm | N.d. | 0.23 |
| mm4_intron_Atoh8_chr6_72217642_F | TCGTCGGCAGCGTCTGGCGGTCTGTTTATTGGCA | 60.2 | 0.22 |
| mm4_intron_Atoh8_chr6_72217642_R | GTCTCGTGGGCTCGGTAAAGTGAGGCCAGCAAGCA | 59.8 | 0.22 |
| mm4_intergenic_Olfr16|Olfr1408_chr1_172964795_F | Primer3: not found at this Tm | N.d. | 0.21 |
| mm4_intergenic_Olfr16|Olfr1408_chr1_172964795_R | Primer3: not found at this Tm | N.d. | 0.21 |
| mm4_intergenic_Dsel|Gm22331_chr1_111990060_F | Primer3: not found at this Tm | N.d. | 0.20 |
| mm4_intergenic_Dsel|Gm22331_chr1_111990060_R | Primer3: not found at this Tm | N.d. | 0.20 |
| mm4_intron_Wdfy3_chr5_101891208_F | Primer3: not found at this Tm | N.d. | 0.20 |
| mm4_intron_Wdfy3_chr5_101891208_R | Primer3: not found at this Tm | N.d. | 0.20 |
| mm4_intron_Ttn_chr2_76876207_F | Primer3: not found at this Tm | N.d. | 0.18 |
| mm4_intron_Ttn_chr2_76876207_R | Primer3: not found at this Tm | N.d. | 0.18 |
| mm3_intron_A830018L16Rik_chr1_11940254_F | TCGTCGGCAGCGTCTCCGTGGATATTGTGTGCTGT | 59.7 | 0.18 |
| mm3_intron_A830018L16Rik_chr1_11940254_R | GTCTCGTGGGCTCGGCACAATCCTGAAAATTCTCAAAGCA | 58.7 | 0.18 |
| mm4_intergenic_Gm24910|Gm25784_chr7_40452377_F | Primer3: not found at this Tm | N.d. | 0.18 |
| mm4_intergenic_Gm24910|Gm25784_chr7_40452377_R | Primer3: not found at this Tm | N.d. | 0.18 |
| mm4_intron_Ywhah_chr5_33020419_F | TCGTCGGCAGCGTCGGGAACATTCTAGGTTTGGGGT | 59.9 | 0.18 |
| mm4_intron_Ywhah_chr5_33020419_R | GTCTCGTGGGCTCGGGGTGGCTTTCAGGGTACACT | 59.5 | 0.18 |
| mm4_intron_Kntc1_chr5_123791844_F | Primer3: not found at this Tm | N.d. | 0.18 |
| mm4_intron_Kntc1_chr5_123791844_R | Primer3: not found at this Tm | N.d. | 0.18 |
| mm4_intergenic_Tmem97|D11Bhm181e_chr11_78560703_F | Primer3: not found at this Tm | N.d. | 0.16 |
| mm4_intergenic_Tmem97|D11Bhm181e_chr11_78560703_R | Primer3: not found at this Tm | N.d. | 0.16 |
| mm4_intergenic_Gm23494|Gm15679_chr8_98749811_F | TCGTCGGCAGCGTCTCTCCAGAAATCCTCTTTCCTCT | 58.3 | 0.16 |
| mm4_intergenic_Gm23494|Gm15679_chr8_98749811_R | GTCTCGTGGGCTCGGGGAACACAGCATCCTACAGCT | 60.0 | 0.16 |
| mm3_intron_Vmn2r-ps119_chr17_19152583_F | Primer3: not found at this Tm | N.d. | 0.16 |
| mm3_intron_Vmn2r-ps119_chr17_19152583_R | Primer3: not found at this Tm | N.d. | 0.16 |
| mm4_exon_Npepps_chr11_97223016_F | TCGTCGGCAGCGTCGTCCCAGCCCAATCTCTCTC | 59.5 | 0.16 |
| mm4_exon_Npepps_chr11_97223016_R | GTCTCGTGGGCTCGGCGACCTGAGCTGTAACCTGG | 60.1 | 0.16 |
| mm4_intron_Nme8_chr13_19668821_F | TCGTCGGCAGCGTCACAAGCTTTGGTGGGAAATGT | 58.6 | 0.16 |
| mm4_intron_Nme8_chr13_19668821_R | GTCTCGTGGGCTCGGAGAGTCCACTCAGGCTGACT | 59.8 | 0.16 |
| mm4_intergenic_AC164609.1|Ighv1-64_chr12_115501343_F | Primer3: not found at this Tm | N.d. | 0.15 |
| mm4_intergenic_AC164609.1|Ighv1-64_chr12_115501343_R | Primer3: not found at this Tm | N.d. | 0.15 |
| mm4_exon_Rnf20_chr4_49654514_F | TCGTCGGCAGCGTCCCAGCTTGCAGATGACCTCA | 60.0 | 0.15 |
| mm4_exon_Rnf20_chr4_49654514_R | GTCTCGTGGGCTCGGTCTTGCCTGTGCTCGTTTGA | 60.1 | 0.15 |
| mm4_intergenic_Slc16a7|Lrig3_chr10_125676082_F | TCGTCGGCAGCGTCACAAGCTGTCTTCACCGAGG | 59.9 | 0.15 |
| mm4_intergenic_Slc16a7|Lrig3_chr10_125676082_R | GTCTCGTGGGCTCGGTGCCTCTTCTCTTTGGGGAA | 58.5 | 0.15 |
| mm4_exon_Gm15708_chr5_144279857_F | Primer3: not found at this Tm | N.d. | 0.14 |
| mm4_exon_Gm15708_chr5_144279857_R | Primer3: not found at this Tm | N.d. | 0.14 |
| mm4_exon_Dip2b_chr15_100155127_F | Primer3: not found at this Tm | N.d. | 0.14 |
| mm4_exon_Dip2b_chr15_100155127_R | Primer3: not found at this Tm | N.d. | 0.14 |
| mm4_intergenic_Gm4916|Gm26475_chrX_128432070_F | Primer3: not found at this Tm | N.d. | 0.14 |
| mm4_intergenic_Gm4916|Gm26475_chrX_128432070_R | Primer3: not found at this Tm | N.d. | 0.14 |
| mm4_intergenic_Cnga2|Magea4_chrX_72164712_F | Primer3: not found at this Tm | N.d. | 0.14 |
| mm4_intergenic_Cnga2|Magea4_chrX_72164712_R | Primer3: not found at this Tm | N.d. | 0.14 |
| mm4_intergenic_Gm26678|Supt20_chr3_54676590_F | TCGTCGGCAGCGTCAGTGGGAAAATCCAGACATGTCA | 59.9 | 0.13 |
| mm4_intergenic_Gm26678|Supt20_chr3_54676590_R | GTCTCGTGGGCTCGGGAAGAGCCATGCTTCCACCT | 60.0 | 0.13 |
| mm4_intron_Itga2_chr13_114896361_F | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_intron_Itga2_chr13_114896361_R | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_intron_Fbxo21_chr5_117978972_F | TCGTCGGCAGCGTCAGCGTTTTCAAGGCGTTTCA | 59.2 | 0.13 |
| mm4_intron_Fbxo21_chr5_117978972_R | GTCTCGTGGGCTCGGTGGTTCATTCTGCCGTTAACA | 58.1 | 0.13 |
| mm4_intergenic_Maf/Gm15655|Dynlrb2_chr8_116257869_F | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_intergenic_Maf/Gm15655|Dynlrb2_chr8_116257869_R | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_intergenic_Gm22537|Sp8_chr12_118759530_F | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_intergenic_Gm22537|Sp8_chr12_118759530_R | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_intergenic_Cnr1|Gm22010_chr4_33978229_F | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_intergenic_Cnr1|Gm22010_chr4_33978229_R | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_intron_Pde4d_chr13_109878872_F | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_intron_Pde4d_chr13_109878872_R | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_intergenic_Icam2|Ern1_chr11_106389918_F | TCGTCGGCAGCGTCTGGAACAATTTCTCTTGGTGCT | 58.4 | 0.13 |
| mm4_intergenic_Icam2|Ern1_chr11_106389918_R | GTCTCGTGGGCTCGGTGGGGAGGGCGTTTGTTG | 60.2 | 0.13 |
| mm4_intergenic_Tex36|2700050L05Rik_chr7_133635009_F | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_intergenic_Tex36|2700050L05Rik_chr7_133635009_R | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_exon_Zswim5_chr4_116989214_F | Primer3: not found at this Tm | N.d. | 0.12 |
| mm4_exon_Zswim5_chr4_116989214_R | Primer3: not found at this Tm | N.d. | 0.12 |
| mm4_intron_Fgf13_chrX_59405795_F | Primer3: not found at this Tm | N.d. | 0.12 |
| mm4_intron_Fgf13_chrX_59405795_R | Primer3: not found at this Tm | N.d. | 0.12 |
| mm4_exon_Sphkap_chr1_83280347_F | Primer3: not found at this Tm | N.d. | 0.11 |
| mm4_exon_Sphkap_chr1_83280347_R | Primer3: not found at this Tm | N.d. | 0.11 |
| mm3_intergenic_Gm25808|Cd180_chr13_102324232_F | Primer3: not found at this Tm | N.d. | 0.11 |
| mm3_intergenic_Gm25808|Cd180_chr13_102324232_R | Primer3: not found at this Tm | N.d. | 0.11 |
| mm4_exon_Grap/Slc5a10_chr11_61671655_F | TCGTCGGCAGCGTCCCTCCAAAGGCCACTGACTA | 59.0 | 0.11 |
| mm4_exon_Grap/Slc5a10_chr11_61671655_R | GTCTCGTGGGCTCGGGAGATGGATCCTGTGCCGAG | 59.9 | 0.11 |
| mm4_intergenic_Rpl31-ps18|Ins1_chr19_52075506_F | Primer3: not found at this Tm | N.d. | 0.11 |
| mm4_intergenic_Rpl31-ps18|Ins1_chr19_52075506_R | Primer3: not found at this Tm | N.d. | 0.11 |
| mm4_intergenic_P2ry1|B430305J03Rik/Rap2b_chr3_61237908_F | TCGTCGGCAGCGTCTGGCTCCTTTTAGCTGCTGC | 60.9 | 0.11 |
| mm4_intergenic_P2ry1|B430305J03Rik/Rap2b_chr3_61237908_R | GTCTCGTGGGCTCGGAAGTGTGTTCAAGCTGGGGC | 61.1 | 0.11 |
| mm4_intron_Dmd_chrX_84985539_F | TCGTCGGCAGCGTCGAGATTTTGTTTTCCCCGTTGG | 58.3 | 0.11 |
| mm4_intron_Dmd_chrX_84985539_R | GTCTCGTGGGCTCGGCCATTCAGCACACAGTAAATGCA | 60.0 | 0.11 |
| mm4_intergenic_Gm13255|Gm13217_chr2_8895318_F | TCGTCGGCAGCGTCTTCCTAAAGAGTGGCATGCA | 57.1 | 0.10 |
| mm4_intergenic_Gm13255|Gm13217_chr2_8895318_R | GTCTCGTGGGCTCGGTGTGATCAGAGAAGTTGCACT | 57.2 | 0.10 |
| mm3_exon_Nadk2_chr15_9109062_F | TCGTCGGCAGCGTCAGTCCGATAGCCCCTTTCCT | 60.0 | 0.10 |
| mm3_exon_Nadk2_chr15_9109062_R | GTCTCGTGGGCTCGGTGCCCAATACTAAAGGAAGGGT | 59.0 | 0.10 |
| mm4_intergenic_Hs3st3a1|Gm24275_chr11_64580280_F | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intergenic_Hs3st3a1|Gm24275_chr11_64580280_R | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_exon_Olfr894_chr9_38219725_F | TCGTCGGCAGCGTCGTGCCCATGTTGAACCCTCT | 60.2 | 0.09 |
| mm4_exon_Olfr894_chr9_38219725_R | GTCTCGTGGGCTCGGACACCATAGCATAAATCAGCTTGT | 58.8 | 0.09 |
| mm4_intergenic_Gm14253|Cnbd2_chr2_156351053_F | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intergenic_Gm14253|Cnbd2_chr2_156351053_R | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intron_Vmn2r99_chr17_19375391_F | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intron_Vmn2r99_chr17_19375391_R | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intergenic_Vmn2r-ps116|Vmn2r-ps117_chr17_18821538_F | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intergenic_Vmn2r-ps116|Vmn2r-ps117_chr17_18821538_R | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intergenic_Vmn2r-ps114|Vmn2r96_chr17_18580692_F | TCGTCGGCAGCGTCAGGGGAGTGAATCAAGAGAATCT | 58.6 | 0.08 |
| mm4_intergenic_Vmn2r-ps114|Vmn2r96_chr17_18580692_R | GTCTCGTGGGCTCGGGCATATTTGCCTGCTGGATTTCT | 59.9 | 0.08 |
| mm4_intron_Pcdh12_chr18_38275059_F | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intron_Pcdh12_chr18_38275059_R | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intron_Trps1_chr15_50728188_F | TCGTCGGCAGCGTCTCACTGGTAGACATGCTCTCTC | 58.9 | 0.08 |
| mm4_intron_Trps1_chr15_50728188_R | GTCTCGTGGGCTCGGTGACTGACAGCAGAAGAATACTGT | 59.7 | 0.08 |
| mm4_intron_Sema6d_chr2_124559342_F | TCGTCGGCAGCGTCAGGGGTGAAAGGAGTGTCCA | 60.4 | 0.08 |
| mm4_intron_Sema6d_chr2_124559342_R | GTCTCGTGGGCTCGGACTTCTAGTCCTCAGCTAGGGA | 59.1 | 0.08 |
| mm4_intergenic_2310057J18Rik|Gm9824_chr10_29002568_F | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intergenic_2310057J18Rik|Gm9824_chr10_29002568_R | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_exon_Hydin_chr8_110513124_F | TCGTCGGCAGCGTCGGAAGAGCGGCATCTCACAA | 60.3 | 0.07 |
| mm4_exon_Hydin_chr8_110513124_R | GTCTCGTGGGCTCGGCCTCCAGCAGTGCTCAGTTA | 59.3 | 0.07 |
| mm4_intergenic_Gm25813|Gm23666_chr8_52993311_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_Gm25813|Gm23666_chr8_52993311_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm3_intron_Nrxn3_chr12_89949883_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm3_intron_Nrxn3_chr12_89949883_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_Hivep1|Edn1_chr13_42262615_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_Hivep1|Edn1_chr13_42262615_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_Gm23892|Gm25382_chr10_16261270_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_Gm23892|Gm25382_chr10_16261270_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_Cacna2d3|Selk_chr14_29773943_F | TCGTCGGCAGCGTCAGAACAGTTTCCCCACCTGC | 60.1 | 0.07 |
| mm4_intergenic_Cacna2d3|Selk_chr14_29773943_R | GTCTCGTGGGCTCGGTTAATGCTGGTCCCTGGGTG | 59.6 | 0.07 |
| mm4_intergenic_Gm22622|Gm5570_chr6_60010333_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_Gm22622|Gm5570_chr6_60010333_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_Gm22622|Gm5570_chr6_59798317_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_Gm22622|Gm5570_chr6_59798317_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_Gm25382|Cited2_chr10_17390213_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_Gm25382|Cited2_chr10_17390213_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_Cda|Fam43b/AB041806_chr4_138380967_F | TCGTCGGCAGCGTCTGGCGACACCACTTTGTGAT | 60.1 | 0.06 |
| mm4_intergenic_Cda|Fam43b/AB041806_chr4_138380967_R | GTCTCGTGGGCTCGGCAGCCCACTCATCACAACCT | 59.9 | 0.06 |
| mm4_intergenic_Gm7931|Slit2_chr5_47232740_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_Gm7931|Slit2_chr5_47232740_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_Gm24246|Pik3c3_chr18_29233353_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_Gm24246|Pik3c3_chr18_29233353_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intron_Hs6st3_chr14_119353802_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intron_Hs6st3_chr14_119353802_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_BC021785/G630090E17Rik|BC021785_chr10_39961818_F | TCGTCGGCAGCGTCCCAGCAACCTCAGCTCTCAG | 60.3 | 0.05 |
| mm4_intergenic_BC021785/G630090E17Rik|BC021785_chr10_39961818_R | GTCTCGTGGGCTCGGACCCTGGCCACTGAGACG | 61.3 | 0.05 |
| mm4_exon_Ccdc84_chr9_44413623_F | TCGTCGGCAGCGTCCTCAGGGTTATGGGGCAAGG | 60.1 | 0.04 |
| mm4_exon_Ccdc84_chr9_44413623_R | GTCTCGTGGGCTCGGGAAGTCCATGGTGAAGGGCT | 59.6 | 0.04 |
| mm4_intron_Akap6_chr12_53133764_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intron_Akap6_chr12_53133764_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Olfr220|Fmn2_chr1_174484227_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Olfr220|Fmn2_chr1_174484227_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Gm22227|Zfp703_chr8_26803782_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Gm22227|Zfp703_chr8_26803782_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intron_Auh_chr13_52846233_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intron_Auh_chr13_52846233_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intron_Gfra1_chr19_58311523_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intron_Gfra1_chr19_58311523_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Gm25299|Gm4732_chrX_24021492_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Gm25299|Gm4732_chrX_24021492_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm3_intergenic_Gm24551|Gm14130/Gm14133_chr2_149719963_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm3_intergenic_Gm24551|Gm14130/Gm14133_chr2_149719963_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Disc1|Sipa1l2_chr8_125368052_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Disc1|Sipa1l2_chr8_125368052_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intron_Prune2_chr19_16981278_F | TCGTCGGCAGCGTCCACTGCCCTGACTCACATGT | 59.9 | 0.04 |
| mm4_intron_Prune2_chr19_16981278_R | GTCTCGTGGGCTCGGACTTCCTGTCACCCATGCTG | 59.9 | 0.04 |
| mm4_intron_Akap13_chr7_75525716_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intron_Akap13_chr7_75525716_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Insig1|En2_chr5_28150440_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Insig1|En2_chr5_28150440_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Mfsd1|Iqcj_chr3_67736690_F | TCGTCGGCAGCGTCGACTCTACAACTAGCGTGGGT | 59.1 | 0.03 |
| mm4_intergenic_Mfsd1|Iqcj_chr3_67736690_R | GTCTCGTGGGCTCGGAGGAGTCCCTTCTTTCCATGT | 58.3 | 0.03 |
| mm4_intergenic_Agap1|Gbx2_chr1_89897023_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Agap1|Gbx2_chr1_89897023_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Mir3475|Frmpd3_chrX_140343599_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Mir3475|Frmpd3_chrX_140343599_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Gm11682|Kcnj16_chr11_110693436_F | TCGTCGGCAGCGTCAGTCAGCTCTCCAAAGTCCA | 58.2 | 0.03 |
| mm4_intergenic_Gm11682|Kcnj16_chr11_110693436_R | GTCTCGTGGGCTCGGACACAGTTCAGCAAGTGGGT | 59.7 | 0.03 |
| mm4_intron_Fip1l1_chr5_74570198_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intron_Fip1l1_chr5_74570198_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Rybp|Gm23234_chr6_100451769_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Rybp|Gm23234_chr6_100451769_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Gm23799|Gm25917_chr12_56142566_F | TCGTCGGCAGCGTCGCCCCATTCTTCCACTTCCA | 59.9 | 0.03 |
| mm4_intergenic_Gm23799|Gm25917_chr12_56142566_R | GTCTCGTGGGCTCGGTCCTTGCAGGAAGAAACTATAAACA | 58.4 | 0.03 |
| mm4_intergenic_Wdpcp|Rpl15-ps1_chr11_21944662_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Wdpcp|Rpl15-ps1_chr11_21944662_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Rab11a|Dennd4a_chr9_64810609_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Rab11a|Dennd4a_chr9_64810609_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intron_Tgm3_chr2_130040632_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intron_Tgm3_chr2_130040632_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm2_intron_Ubr1_chr2_120874770_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm2_intron_Ubr1_chr2_120874770_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm3_intergenic_Gm25550|Cdh9_chr15_16519977_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm3_intergenic_Gm25550|Cdh9_chr15_16519977_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Gm2018|4932411E22Rik_chr11_89315539_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Gm2018|4932411E22Rik_chr11_89315539_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_PVT1_3|Ndufa4_chr6_11484580_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_PVT1_3|Ndufa4_chr6_11484580_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_Map7_chr10_20235088_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_Map7_chr10_20235088_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_exon_Kbtbd2_chr6_56780208_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_exon_Kbtbd2_chr6_56780208_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Gm10499|2410017I17Rik_chr17_36154789_F | TCGTCGGCAGCGTCAACAAGTGAGAGCCTGTGGG | 59.8 | 0.01 |
| mm4_intergenic_Gm10499|2410017I17Rik_chr17_36154789_R | GTCTCGTGGGCTCGGGTCCAAGGAACTCCCAGAGC | 60.0 | 0.01 |
| mm4_intron_Gpld1_chr13_24989197_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_Gpld1_chr13_24989197_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Fli1|RP24-88B13.1_chr9_32610558_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Fli1|RP24-88B13.1_chr9_32610558_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_Sept10_chr10_59173528_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_Sept10_chr10_59173528_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Dtna|Gm15972_chr18_23707954_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Dtna|Gm15972_chr18_23707954_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Gm12506|Gm12519_chr4_55923292_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Gm12506|Gm12519_chr4_55923292_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_Abca16_chr7_120434941_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_Abca16_chr7_120434941_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_exon_Gm14935_chrX_96548609_F | TCGTCGGCAGCGTCTGCTCTCACTTGTCGCCAAT | 59.9 | 0.01 |
| mm4_exon_Gm14935_chrX_96548609_R | GTCTCGTGGGCTCGGAGCCCCAGTGAGATGAGGAT | 60.0 | 0.01 |
| mm4_intron_Wdr93_chr7_79761376_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Wdr93_chr7_79761376_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Zfp207|Psmd11_chr11_80428014_F | TCGTCGGCAGCGTCAGAAGGTCTCCCTCTGTATAGGT | 59.2 | 0.00 |
| mm4_intergenic_Zfp207|Psmd11_chr11_80428014_R | GTCTCGTGGGCTCGGTCCTCCCGTATCTCCAATTCCT | 60.0 | 0.00 |
| mm4_intergenic_Vwde|Gm25737_chr6_13302842_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Vwde|Gm25737_chr6_13302842_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Tcerg1l|Mapk1ip1_chr7_138512421_F | TCGTCGGCAGCGTCGTTGGAAGCCCTCAGACCTC | 60.0 | 0.00 |
| mm4_intergenic_Tcerg1l|Mapk1ip1_chr7_138512421_R | GTCTCGTGGGCTCGGAGAGTCAGGGGCTACACCAT | 59.9 | 0.00 |
| mm4_intergenic_Ndufb4|Fstl1_chr16_37737103_F | TCGTCGGCAGCGTCGGTTCTCCCAAACTCTGCGA | 59.9 | 0.00 |
| mm4_intergenic_Ndufb4|Fstl1_chr16_37737103_R | GTCTCGTGGGCTCGGTGAGACATCTCTTTATCCTCACCA | 58.7 | 0.00 |
| mm4_intergenic_Mgst1|Lmo3_chr6_138319582_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Mgst1|Lmo3_chr6_138319582_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Asap1|Adcy8_chr15_64484905_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Asap1|Adcy8_chr15_64484905_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_exon_Camk2b_chr11_5997913_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_exon_Camk2b_chr11_5997913_R | Primer3: not found at this Tm | N.d. | 0.00 |
These only list off-targets that have primers in the table above. Primers underlined, off-targets in bold.
| mm4_intergenic_Gm13478|1700019E08Rik_chr2_45693238 |
ACAAGGCAGGGAAGAAGTGGGAAAAAAAATGAAGGATTCCCAAGAAGAAATCAAGGCATATACTCCTGTATAAAAAAAAA TATAACAAGAAAGACAGGGATATTGTAGCAGA |
| mm3_intron_Clvs1_chr4_9348303 |
AAGAGGAAGGGAGTGTGGGGGAATTTATATGAAGAAATCAAGGATGCAGGAAATGAAGGATATATTGCTGCCAAAGATAA GCAGCAAGGCTTCCGTACCAATGAGC |
| mm3_intron_Ankfn1_chr11_89510951 |
ACACACGCATAAAGGTTGTCAAAGCAAATGCTATCTGTGATTTCTAAGAAGAAAACGAGGTCACTTCAGTATAAACCTTG CTTTGAGAGCCAATATCCTCTGGGCAACCAAGAAAGGCAGTT |
| mm4_intergenic_Gm10247|Grin2a_chr16_9575146 |
CCATTTGCCACTCCCTGGATTAGTTTTTGAGAGGACTCCTTCAAACAAGCCCACATGGTTACCTTTCACTTGGAACCTGA CCACCTCGTTTTCTTCTTTGAAATCATCAATTATAAGAGTCAGTGGATTAGTGGAGGGG |
| mm3_intergenic_Gm23083|Gm24773_chr16_80679807 |
ACCTGAAGTTCATCTGTTAGCACAGTCTGCACAATGATTTTATATGAAGAAATCGTGGTATCGGAAGAGTTCCCATTCAT TTCATTGCT |
| mm4_intron_Gm14569_chrX_36427936 |
ACATGCTTTCTTGAGGTCCGTTTTCTGCAGGTTTTCTAAGAAGAAAACAAGGCATCATATAACTTTCCACCATCCCTGAT CCTCA |
| mm4_intron_Dock5_chr14_67892490 |
TGTGACCACCAAACTTATAAAACCAACTAAACGTTAATAGGAAAATCACATTTCTATGGAGAAATCTAGGCTTGATGGCT CGCTTGTCTTCTGG |
| mm4_intergenic_Gm26651|Diras2_chr13_52128681 |
AGTTCTCACTGACAGCCGTGCTCCCCGTGGTTCGGCAGCCTCTGATCACATTCTCAGCAGGGCTGGAAACCACGATTTCT TCATAAACACCTGCAGGCTGCTGTATTTATTGCAGGCTGCTTGGGAGGTGAATGTGGCAA |
| mm3_intergenic_Trim72|Gm22809_chr7_128011790 |
AGTCTCTCCCATGGCCTCTAGAATGTGGTTTTGTTTGGACATTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNGAAAGAGTTTCACACTGTCTTTCTTTGGGCCCTGCTG |
| mm3_intergenic_Cdh7|Cdh19_chr1_110274873 |
ACTCAAGTACCAATGCATCTGAGCATTTCTAAGAAGAAATTGGGGAAATGACTATGAGAATTGTAGAAATTATGTATTGG TGAAACGAGTAAGTTTAGTACCTTTCAAACAACATTACTGTATAACCATGCCTCAGG |
| mm3_intergenic_mmu-mir-7689|Sorcs2_chr5_36311543 |
GAGCCACACTCTGCTCACAGCAAGAAGCAGTTCCCATCTTTCTCTGGGGTGACTGGCCAAGATTTCTTGATAGAAATCTT TTTGCAGCTTAATACCTCTCAAGACTAGAACTGCCTAACCCAGATGCAAGGT |
| mm4_intron_Mettl24_chr10_40790174 |
TCCAAACTGTAATAGATGGGGATCAATAACTTCTAATAGATATAAATTATTAGTCTGTTGCACTGATGCCCACTATTTCT TCATGGAAGTCTTCAGCTGTGCACAACTTTTAAA |
| mm4_intergenic_Uncx|Micall2_chr5_139697920 |
GCTGCTTCTGTGACACCAATGTGGAGGTGCTGGCCTCTGCGTGTCCTGGGGCCTTGCCAGCACATCTATTTGCCTTCTCA GGGCTCCACTGCCCCCACGATTTCCTCATAGAGCCCCTGAGCATCCCCTTGTATCCCTCG |
| mm4_intergenic_Gm11680|BC006965_chr11_112598796 |
GGCTGGCTTTGCATCTAGGAAACTTCCAAAAGTTCCTAAAGTACTCCAAAACAAAGTTCTTTGGTATTGGCTCTCTTTGA AGAAATCAAGGACCCATTGAAGGTCAAGTTCCAGTATCCATCAGACCATCCATAGGGACC |
| mm4_intron_Atoh8_chr6_72217642 |
TGGCGGTCTGTTTATTGGCATTCTTTTGAGCCAAATGTTGCCTAGATTTCTTGATATAGATCCACGTCTTTTGAAATCGG GGATTTATGGACTGGCTGCTTGCTGGCCTCACTTTA |
| mm3_intron_A830018L16Rik_chr1_11940254 |
TCCGTGGATATTGTGTGCTGTGTTTTGATTGAGAAGCTCCAGCCTTTGATTTCTTCTTAGAAATCTAAAACCATTTTTCA GTTTTCCTTCTTTAAAAACAGAGGACTTCAAAATATGCTTTGAGAATTTTCAGGATTGTG |
| mm4_intron_Ywhah_chr5_33020419 |
GGGAACATTCTAGGTTTGGGGTATGAATTTTTCTGAAGAAATTGTGGATTCTTTGAGAAAATGAATTTAAAATAACAGTT TTGCAAAATTTTTTGGAAACAAGACTGATATTACTAAAGTGTACCCTGAAAGCCACC |
| mm4_intergenic_Gm23494|Gm15679_chr8_98749811 |
TCTCCAGAAATCCTCTTTCCTCTACTTTTCCATGTTTTCATCATAGAAATCTGTCTTTGTCTTCACTATGTCATATACCA AGTTCTCAAATCAGTTCTGTGTACCGACTGTTCATCAGCTGTAGGATGCTGTGTTCC |
| mm4_exon_Npepps_chr11_97223016 |
GTCCCAGCCCAATCTCTCTCCTATAGGTGAAAAGACATCTTTGACAAACTCCTGGATTTCCTCATAGAAGTCTGTGTGGG ACAAGAGAGTTGAAAGAATCCCCAGGTTACAGCTCAGGTCG |
| mm4_intron_Nme8_chr13_19668821 |
ACAAGCTTTGGTGGGAAATGTAGAGAATGAGCTCTGATTTCTCTGAGGAAATAGAGGTGATGAAAATGAACGACAAGAAA TGCTTTTATCGTTTACATTACTGTGTTTGTCCCCTACCACAGTCAGCCTGAGTGGACTCT |
| mm4_exon_Rnf20_chr4_49654514 |
CCAGCTTGCAGATGACCTCAAAGCACAGCTAGAACTGGCTCAGAAGAAGCTCCATGATTTCCAGGATGAAATCGTGGAGA ACAGTGTCACCAAAGAAAAGGACTTGTTCAATTTCAAACGAGCACAGGCAAGA |
| mm4_intergenic_Slc16a7|Lrig3_chr10_125676082 |
ACAAGCTGTCTTCACCGAGGCAAGAACAGCAGACTTGAGGCAGATTGCTAGCTGAAGGTTTTTCTATAAAGAAAGCGAGG AAAAGAAGTAGGGGGCATTACTGTGGTTTGAAAACAACCTTTCCCCAAAGAGAAGAGGCA |
| mm4_intergenic_Gm26678|Supt20_chr3_54676590 |
AGTGGGAAAATCCAGACATGTCATAAACTGCACAGAAATTCAATGAAGAAATAGAGGACTTTGCAAAATAGTATCTGTAT CCTTTCAATACTAAAGGGAAAGGTGGAAGCATGGCTCTTC |
| mm4_intron_Fbxo21_chr5_117978972 |
AGCGTTTTCAAGGCGTTTCAAGTTTTGAAAGAGCAGATCTGAACTTTGAACACATTTTTAACTCAGTCCCCAATGGGAAC TACAAATAGACACCCCCCAATTTGTTTATAGAAATGCTTGTTAACGGCAGAATGAACCA |
| mm4_intergenic_Icam2|Ern1_chr11_106389918 |
TGGAACAATTTCTCTTGGTGCTTTTGTGCCTCAATTTCTTCAAAGCAATTCCCCCCACCCCCCAACAAACGCCCTCCCCA |
| mm4_exon_Grap/Slc5a10_chr11_61671655 |
CCTCCAAAGGCCACTGACTATTCTGCCCCCTATTTCTCCATAGCCATCCCGGGCTTGCTTTGCCCAGGCCCAGTTTGACT TCTCGGCACAGGATCCATCTC |
| mm4_intergenic_P2ry1|B430305J03Rik/Rap2b_chr3_61237908 | TGGCTCCTTTTAGCTGCTGCTCCTCAATTTATTCATAGGAATCAGAAAGCCCAGAAGCCCCAGCTTGAACACACTT |
| mm4_intron_Dmd_chrX_84985539 |
GAGATTTTGTTTTCCCCGTTGGCCACTTTTTCTTCATAGAAAGCTTTTTGTAAACATATTTTTTTCATAGTCATGATCTA TCGAATTTTCTACCCAAAAAGAAAATCCAAGTTAATATGCATTTACTGTGTGCTGAATGG |
| mm4_intergenic_Gm13255|Gm13217_chr2_8895318 |
TTCCTAAAGAGTGGCATGCATATCATTCTAAGGATTTAGATCAAGAAATTGTGGGCCAAGTATTTAGTGCAACTTCTCTG ATCACA |
| mm3_exon_Nadk2_chr15_9109062 |
AGTCCGATAGCCCCTTTCCTGATTTTGGTTTTGGATTTCTATTATGAAATCAAGGATTAGAAGGATAATCAATGATTGTG CTCTGTTCAGTAAAATGTTCAAAGACCCTTCCTTTAGTATTGGGCA |
| mm4_exon_Olfr894_chr9_38219725 |
GTGCCCATGTTGAACCCTCTTATTTATAGCCTCAGAAACAAGGATGTCAAGCTTGCTATGAAGAAATCCTGGAAAAGAAT TACAAGCTGATTTATGCTATGGTGT |
| mm4_intergenic_Vmn2r-ps114|Vmn2r96_chr17_18580692 |
AGGGGAGTGAATCAAGAGAATCTCTACAGGTTTCCTATGAAGAGATCTTGGCATATGTGTTAAGAAAATTAAGAAATCCA GCAGGCAAATATGC |
| mm4_intron_Trps1_chr15_50728188 |
TCACTGGTAGACATGCTCTCTCTCTCTTTTTTTTTTAAGTCTCAAAAAAAGAGCTGATGCATTCCAAGATCTCTTCATGG AAATACAAGTTCAATGGAAAAAAAATAAAACAGTATTCTTCTGCTGTCAGTCA |
| mm4_intron_Sema6d_chr2_124559342 |
AGGGGTGAAAGGAGTGTCCATAGAAAGGATATGACTCCCTTGTTTGCTTCCTAGAAATCCAGCTTGGCAAGTTTTGAATG ACTGTGATGACATGTTGACTTCTCATCCCTAGCTGAGGACTAGAAGT |
| mm4_exon_Hydin_chr8_110513124 |
GGAAGAGCGGCATCTCACAATTGAAGCCAAAAACTACCTAGATGAGGATGACTATGAAGAAAGCCTGGAAAAACTAACAT TTCAAAGTAACTGAGCACTGCTGGAGG |
| mm4_intergenic_Cacna2d3|Selk_chr14_29773943 |
AGAACAGTTTCCCCACCTGCCAACGCTCTTTAAAACTAGAGCTCTGGCTTTCACTATCTGGTTACAGCTGGGTCTCTCCA GCCTGGGCCCAGTTTTCTGCATAGCAATCCTGTGCTCACCCAGGGACCAGCATTAA |
| mm4_intergenic_Cda|Fam43b/AB041806_chr4_138380967 |
TGGCGACACCACTTTGTGATGCTTTCATGGAGATCTAGGAAGAAATAGTGGGGTGGAAATGGAGGTTGTGATGAGTGGGC TG |
| mm4_intergenic_BC021785/G630090E17Rik|BC021785_chr10_39961818 |
CCAGCAACCTCAGCTCTCAGCATGTCTCAACAACTGCAAATATTTCAAACTTCAGAAAGCAAGGAGATAACAGAGGAATC TGTATGAAGAAATGGAGGCTGAGAACTGTGTTCTGGTGCCCGTCTCAGTGGCCAGGGT |
| mm4_exon_Ccdc84_chr9_44413623 |
CTCAGGGTTATGGGGCAAGGTCTCTCAGAGGCCTTTCTTCCTACCTCTTTAATCACCTCGTCCTCCTTTTCCTCATAGGA ATCCAAGCCCTTCACCATGGACTTC |
| mm4_intron_Prune2_chr19_16981278 |
CACTGCCCTGACTCACATGTAGACTCCATGTCTTCTGGCTTTCTCTGAAGAAGTCATGGGGAGGAGCCCCACAGGGCAGC AGAGCTATGGGGAGCAAGGCCCTCCCTGATGCAGCATGGGTGACAGGAAGT |
| mm4_intergenic_Mfsd1|Iqcj_chr3_67736690 |
GACTCTACAACTAGCGTGGGTTACTGGGATTTCTATGAGCTAATCTGGGTTGCTACTCAGTGTTATTATAACGTGAGGTT TCATTAACTATTCCATTGTCAAATATAAAAGTATAACATGGAAAGAAGGGACTCCT |
| mm4_intergenic_Gm11682|Kcnj16_chr11_110693436 |
AGTCAGCTCTCCAAAGTCCAAATAATAATTGATGTCCAACATTTCTTCGTAGAAAACCCAATTTTATAAAACCCACTTGC TGAACTGTGT |
| mm4_intergenic_Gm23799|Gm25917_chr12_56142566 |
GCCCCATTCTTCCACTTCCAAAACAATGCCCTGGAGGTCCAGGATGTCTATGAAAAATTTGAGGCTTTTGTTGCTCAAAT AAAACTTGTTTATAGTTTCTTCCTGCAAGGA |
| mm4_intergenic_Gm10499|2410017I17Rik_chr17_36154789 |
AACAAGTGAGAGCCTGTGGGTCCTCAAGGTCAGCCAAGCCCTCCCTTCCTTCCTAGAAATCCCCTAAACTAAAAGTGAAA GTAGGTCCCAGGTCTTGGGTCCCAGGGGGTCCGGCAAGCTCTGGGAGTTCCTTGGAC |
| mm4_exon_Gm14935_chrX_96548609 |
TGCTCTCACTTGTCGCCAATGGCCTGCCTTGCCAGGAGCACTCCCACCACTTCTTCACAGAAATGCTTCAACTACCCACA GTCCCTTAGCCAGATGAGATCAGTGTTCTGGGCATTGGATCCTCATCTCACTGGGGCT |
| mm4_intergenic_Zfp207|Psmd11_chr11_80428014 |
AGAAGGTCTCCCTCTGTATAGGTTTAGCAACATCTTTAGGTTGATCCTTAGTGATAAGAAACATGCTTTTTTCCCCTTTC CCTTGATATCTTCACAGATATCCTATTACAGTTGAAGGAATTGGAGATACGGGAGGA |
| mm4_intergenic_Tcerg1l|Mapk1ip1_chr7_138512421 |
GTTGGAAGCCCTCAGACCTCAGGCATGGACTGCTATGAAGATTTCGTGGTTTTAACTACTCTGAGCCTTAAGCCCAAGTC TCAGTTTACTCGTCTGACATTAAAATGGTGTAGCCCCTGACTCT |
| mm4_intergenic_Ndufb4|Fstl1_chr16_37737103 | GGTTCTCCCAAACTCTGCGATTTGATGGAATTTCTATGAAGATTTCTAGGTGGTGAGGATAAAGAGATGTCTCA |