CRISPOR

DNA break repair outcome predictions

Guide: ATCCACTTGCGTTCGCCGAG TGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

mh- Score	Sequence	Effect
	`TGTGGCAGACGTTTCTATTTATCCACTTGCGTTCGCCGAGTGGCGTCACCAGCGGTACTGTAATGACGATTGCAGCAGGA`	(Wild-type sequence)
347	`GTTTCTATTTATCCACTTGCGT--------------CACCAGCGGTACTGTAATGACGAT`	`14 bp deleted → frameshift`
282	`GTTTCTATTTATCCACTTGCGTTCGCCG-------TCACCAGCGGTACTGTAATGACGAT`	`7 bp deleted → frameshift`
268	`GTTTCTATTTATCCACTTGCGTTCGC--------GTCACCAGCGGTACTGTAATGACGAT`	`8 bp deleted → frameshift`
242	`GTTTCTATTTATCCACTTGCGTTCG----------TCACCAGCGGTACTGTAATGACGAT`	`10 bp deleted → frameshift`
208	`GTTTCTATTTATCCACTTGCGTTCGCC-------------AGCGGTACTGTAATGACGAT`	`13 bp deleted → frameshift`
190	`GTTTCTATTTATCCACTTGCG-----------------------GTACTGTAATGACGAT`	`23 bp deleted → frameshift`
179	`GTTTCTATTTATCCACTTGCGTTCGCCG----------------GTACTGTAATGACGAT`	`16 bp deleted → frameshift`
170	`GTTTCTATTTATCCACTTGCGTTCGC-----------------GGTACTGTAATGACGAT`	`17 bp deleted → frameshift`
164	`GTTTCTATTTATCCACTTGCGTTCGCCGAG------------CGGTACTGTAATGACGAT`	`12 bp deleted → no frameshift`
158	`GTTTCTATTTATCCAC-----------------------CAGCGGTACTGTAATGACGAT`	`23 bp deleted → frameshift`
156	`GTTTCTATTTATCCACTTGCGTTC-------------ACCAGCGGTACTGTAATGACGAT`	`13 bp deleted → frameshift`
156	`GTTTCTATTTATCCACTTG-------------GCGTCACCAGCGGTACTGTAATGACGAT`	`13 bp deleted → frameshift`
154	`GTTTCTATTTATCCACTTGCGTTCG-------------------GTACTGTAATGACGAT`	`19 bp deleted → frameshift`
136	`GTTTCTATTTATCCA--------------------------GCGGTACTGTAATGACGAT`	`26 bp deleted → frameshift`
111	`GTTTCTATTTATCCACTTGCGTTCGCCGA------------------------------T`	`30 bp deleted → no frameshift`
90	`GTTTCTATTTATCCACTTGCGT------------------------ACTGTAATGACGAT`	`24 bp deleted → no frameshift`
90	`GTTTCTATTTATC------------------------ACCAGCGGTACTGTAATGACGAT`	`24 bp deleted → no frameshift`
80	`GTTTCTATTTATCCACT--------------------------------GTAATGACGAT`	`32 bp deleted → frameshift`
77	`GTTTCTATTTATCCACTTGCGTTCGCCGA---------------------------CGAT`	`27 bp deleted → no frameshift`
76	`GTTTCTATTTATCCACTTGCGTTCG---------------------------------AT`	`33 bp deleted → no frameshift`
70	`GTTTCTATTTATCCACTTGCGT-----------------------------AATGACGAT`	`29 bp deleted → frameshift`
63	`GTTTCTATTTATCCACTTG-------------------------------TAATGACGAT`	`31 bp deleted → frameshift`
62	`GTTTCTATTTATCCACTTGCG-------------------------------------AT`	`37 bp deleted → frameshift`
60	`GTTTC--------------------------------ACCAGCGGTACTGTAATGACGAT`	`32 bp deleted → frameshift`
54	`GT----------------------------------CACCAGCGGTACTGTAATGACGAT`	`34 bp deleted → frameshift`
49	`GTTTCTATTTATCCACTTG------------------------------------ACGAT`	`36 bp deleted → no frameshift`
38	`GTTTCTATTTATCCAC-----------------------------------------GAT`	`41 bp deleted → frameshift`
34	`GTTTCT-------------------------------------------GTAATGACGAT`	`43 bp deleted → frameshift`
33	`GT--------------------------------------------ACTGTAATGACGAT`	`44 bp deleted → frameshift`
33	`GTTTCTATTTA------------------------------------CTGTAATGACGAT`	`36 bp deleted → no frameshift`
27	`GTTTCTA----------------------------------------CTGTAATGACGAT`	`40 bp deleted → frameshift`
25	`GTTTCTATTTA-----------------------------------------ATGACGAT`	`41 bp deleted → frameshift`
25	`GT-------------------------------------------------AATGACGAT`	`49 bp deleted → frameshift`
24	`GTTTCTATTTAT------------------------------------------GACGAT`	`42 bp deleted → no frameshift`
21	`GTTTCTA---------------------------------------------ATGACGAT`	`45 bp deleted → no frameshift`
20	`GTTTCTAT----------------------------------------------GACGAT`	`46 bp deleted → frameshift`
18	`GTTTCTATTTAT------------------------------------------------`	`48 bp deleted → no frameshift`
14	`GTTTCTAT----------------------------------------------------`	`52 bp deleted → frameshift`

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