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Contents:In the table below, Illumina Nextera Handle sequences have been added and highlighted in bold. Primers for the on-target have been added for convenience. The table below is sorted by the CFD off-target score. Sites with very low CFD scores < 0.02 are unlikely to be cleaved, see our study Haeussler et al. 2016, Figure 2.
In the protocol by Matthew Canver, Harvard, two PCRs are run: one PCR to amplify the potential off-target, then a second PCR to extend the handles with Illumina barcodes. Please click here to download the protocol. Alternatively, you can have a look at Fu et al, 2014.
If a primer was not found, the reason is usually that the region around the off-target is too repetitive. To avoid unspecific primers, all repeats are masked for the primer design (not for off-target search). If you think that we should change the parameters here or should use different primer3 settings, please let us know.
| Name | Primer Sequence | Tm | CFD Score |
|---|---|---|---|
| ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52314003_F | TCGTCGGCAGCGTCAACCGCCAGAGAAGATGGTG | 60.0 | 1.00 |
| ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52314003_R | GTCTCGTGGGCTCGGCTGCCTGCTAGTCCATCGAC | 60.2 | 1.00 |
| mm4_intron_Frmd4a_chr2_4598953_F | TCGTCGGCAGCGTCCAGTTTTGCCCAACGTCCTG | 59.9 | 0.42 |
| mm4_intron_Frmd4a_chr2_4598953_R | GTCTCGTGGGCTCGGACCGCAAGTAGGAACACAGG | 59.9 | 0.42 |
| mm4_intergenic_Akt2-ps|Gm12663_chr11_16559605_F | Primer3: not found at this Tm | N.d. | 0.41 |
| mm4_intergenic_Akt2-ps|Gm12663_chr11_16559605_R | Primer3: not found at this Tm | N.d. | 0.41 |
| mm4_intergenic_Gm25261|Gm831_chr4_131377771_F | Primer3: not found at this Tm | N.d. | 0.37 |
| mm4_intergenic_Gm25261|Gm831_chr4_131377771_R | Primer3: not found at this Tm | N.d. | 0.37 |
| mm4_intergenic_Tcf23|Slc5a6_chr5_30993697_F | Primer3: not found at this Tm | N.d. | 0.32 |
| mm4_intergenic_Tcf23|Slc5a6_chr5_30993697_R | Primer3: not found at this Tm | N.d. | 0.32 |
| mm3_intergenic_Arap3|Pcdh1_chr18_38106270_F | Primer3: not found at this Tm | N.d. | 0.32 |
| mm3_intergenic_Arap3|Pcdh1_chr18_38106270_R | Primer3: not found at this Tm | N.d. | 0.32 |
| mm3_intergenic_Tmem30b|Prkch_chr12_73580600_F | Primer3: not found at this Tm | N.d. | 0.30 |
| mm3_intergenic_Tmem30b|Prkch_chr12_73580600_R | Primer3: not found at this Tm | N.d. | 0.30 |
| mm4_intergenic_Ubr5|Odf1_chr15_38178298_F | TCGTCGGCAGCGTCCCAGAAGCAGAAGTCCAGCA | 59.9 | 0.25 |
| mm4_intergenic_Ubr5|Odf1_chr15_38178298_R | GTCTCGTGGGCTCGGCCCCACCCCGAGAAAATCAT | 59.7 | 0.25 |
| mm4_intergenic_Alk|Gm24736_chr17_72396371_F | Primer3: not found at this Tm | N.d. | 0.25 |
| mm4_intergenic_Alk|Gm24736_chr17_72396371_R | Primer3: not found at this Tm | N.d. | 0.25 |
| mm3_intergenic_Otogl|Ppp1r12a_chr10_107951530_F | Primer3: not found at this Tm | N.d. | 0.25 |
| mm3_intergenic_Otogl|Ppp1r12a_chr10_107951530_R | Primer3: not found at this Tm | N.d. | 0.25 |
| mm4_intergenic_Hnf4a|Ttpal_chr2_163593482_F | Primer3: not found at this Tm | N.d. | 0.25 |
| mm4_intergenic_Hnf4a|Ttpal_chr2_163593482_R | Primer3: not found at this Tm | N.d. | 0.25 |
| mm4_intron_Gm26962_chr9_115195834_F | Primer3: not found at this Tm | N.d. | 0.21 |
| mm4_intron_Gm26962_chr9_115195834_R | Primer3: not found at this Tm | N.d. | 0.21 |
| mm4_intergenic_Tcrg-C3|Tcrg-C2_chr13_19293242_F | Primer3: not found at this Tm | N.d. | 0.20 |
| mm4_intergenic_Tcrg-C3|Tcrg-C2_chr13_19293242_R | Primer3: not found at this Tm | N.d. | 0.20 |
| mm4_intergenic_Tfcp2l1/Gm23497|Gli2_chr1_118801853_F | Primer3: not found at this Tm | N.d. | 0.19 |
| mm4_intergenic_Tfcp2l1/Gm23497|Gli2_chr1_118801853_R | Primer3: not found at this Tm | N.d. | 0.19 |
| mm4_exon_Gm15917_chr10_81572801_F | TCGTCGGCAGCGTCAGACACCAAAAGCCGGGAAT | 59.8 | 0.18 |
| mm4_exon_Gm15917_chr10_81572801_R | GTCTCGTGGGCTCGGCTTGCAGACCGAGGAACACA | 60.2 | 0.18 |
| mm4_intron_Gm26665_chr1_166003011_F | Primer3: not found at this Tm | N.d. | 0.14 |
| mm4_intron_Gm26665_chr1_166003011_R | Primer3: not found at this Tm | N.d. | 0.14 |
| mm4_exon_Ctbp2_chr7_133123831_F | TCGTCGGCAGCGTCGTTTTCTCCTCCCGGGAAGG | 60.0 | 0.11 |
| mm4_exon_Ctbp2_chr7_133123831_R | GTCTCGTGGGCTCGGGACCCTCACCTCGCCTCC | 61.4 | 0.11 |
| mm3_intergenic_Tug1/TUG1_2/TUG1_1|Tug1_chr11_3648646_F | Primer3: not found at this Tm | N.d. | 0.11 |
| mm3_intergenic_Tug1/TUG1_2/TUG1_1|Tug1_chr11_3648646_R | Primer3: not found at this Tm | N.d. | 0.11 |
| mm4_intron_Fam78a_chr2_32073731_F | TCGTCGGCAGCGTCAGCCTTGACACTCAGCCTTC | 59.9 | 0.11 |
| mm4_intron_Fam78a_chr2_32073731_R | GTCTCGTGGGCTCGGTCAAAGCAGCCTCCAGGTTT | 59.8 | 0.11 |
| mm4_intergenic_Gm6265|Nkx2-1_chr12_56519827_F | Primer3: not found at this Tm | N.d. | 0.11 |
| mm4_intergenic_Gm6265|Nkx2-1_chr12_56519827_R | Primer3: not found at this Tm | N.d. | 0.11 |
| mm4_intron_Dag1_chr9_108263340_F | Primer3: not found at this Tm | N.d. | 0.11 |
| mm4_intron_Dag1_chr9_108263340_R | Primer3: not found at this Tm | N.d. | 0.11 |
| mm4_intergenic_Slc22a16|Ddo_chr10_40629889_F | TCGTCGGCAGCGTCGAGGCAGTTCCACAGAGCTT | 59.9 | 0.10 |
| mm4_intergenic_Slc22a16|Ddo_chr10_40629889_R | GTCTCGTGGGCTCGGATTGGTCCATGCCACAGTGT | 59.8 | 0.10 |
| mm4_exon_Gm12827/Rnf220_chr4_117496956_F | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_exon_Gm12827/Rnf220_chr4_117496956_R | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intergenic_Spata19|Opcml_chr9_27573118_F | TCGTCGGCAGCGTCAGGGTGTCTGGAACATACAGA | 58.0 | 0.10 |
| mm4_intergenic_Spata19|Opcml_chr9_27573118_R | GTCTCGTGGGCTCGGTCCAGGACTGACCATCACTAGA | 59.6 | 0.10 |
| mm4_intergenic_Arf2|Crhr1_chr11_104030390_F | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intergenic_Arf2|Crhr1_chr11_104030390_R | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intergenic_Onecut1/Gm20649|Fam214a_chr9_74899306_F | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intergenic_Onecut1/Gm20649|Fam214a_chr9_74899306_R | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intergenic_Runx2|Supt3_chr17_44829481_F | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intergenic_Runx2|Supt3_chr17_44829481_R | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_exon_Chic2_chr5_75044628_F | TCGTCGGCAGCGTCCAACCACAGCAACAGCCC | 59.2 | 0.08 |
| mm4_exon_Chic2_chr5_75044628_R | GTCTCGTGGGCTCGGGAGACCGTCCGCGTGAAC | 60.8 | 0.08 |
| mm4_intergenic_6030443J06Rik|Gm21846_chr5_22746026_F | TCGTCGGCAGCGTCCAGGAGGGAGGGAAGAGGG | 60.3 | 0.08 |
| mm4_intergenic_6030443J06Rik|Gm21846_chr5_22746026_R | GTCTCGTGGGCTCGGCCCAGCAGCACCAGTTCTAG | 60.3 | 0.08 |
| mm4_exon_Txlna_chr4_129640255_F | TCGTCGGCAGCGTCGAAAAGCAGAGCTGGCACAC | 60.0 | 0.08 |
| mm4_exon_Txlna_chr4_129640255_R | GTCTCGTGGGCTCGGGACCCAGACAGACAGCTCCT | 60.9 | 0.08 |
| mm4_intron_Dnajc13_chr9_104236432_F | TCGTCGGCAGCGTCGTAAAGCCCATGCACGGTTG | 60.1 | 0.07 |
| mm4_intron_Dnajc13_chr9_104236432_R | GTCTCGTGGGCTCGGGTGCAGTCCTGACTTTCCCA | 59.8 | 0.07 |
| mm3_intergenic_Igdcc3|Gm25313_chr9_65188730_F | TCGTCGGCAGCGTCAAGTTGGTGCAGGAGAGCAG | 60.2 | 0.07 |
| mm3_intergenic_Igdcc3|Gm25313_chr9_65188730_R | GTCTCGTGGGCTCGGCAAGGTGGCAGCAAAAGTCC | 59.9 | 0.07 |
| mm4_exon_Thumpd3_chr6_113046262_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_exon_Thumpd3_chr6_113046262_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intron_Aplf_chr6_87640140_F | TCGTCGGCAGCGTCCACGGTCACTCCTGCATCTA | 59.1 | 0.06 |
| mm4_intron_Aplf_chr6_87640140_R | GTCTCGTGGGCTCGGCCTTTCCACCAGAGGCATCA | 59.6 | 0.06 |
| mm4_intergenic_Lipc|Aqp9_chr9_70963548_F | TCGTCGGCAGCGTCAATGAGAGCAGCTTCCGCTC | 60.4 | 0.06 |
| mm4_intergenic_Lipc|Aqp9_chr9_70963548_R | GTCTCGTGGGCTCGGACTCCAAGACACAGTGCCTG | 59.8 | 0.06 |
| mm4_intergenic_Tcrg-C3|Tcrg-C2_chr13_19283309_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_Tcrg-C3|Tcrg-C2_chr13_19283309_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_Tcrg-C1|Tcrg-V3_chr13_19236504_F | TCGTCGGCAGCGTCAGCTGCACCATTGTCTCCTC | 60.0 | 0.06 |
| mm4_intergenic_Tcrg-C1|Tcrg-V3_chr13_19236504_R | GTCTCGTGGGCTCGGGGGCACTACTCTGCAGCAAT | 60.3 | 0.06 |
| mm4_intron_Inpp4a_chr1_37366459_F | TCGTCGGCAGCGTCTGTCATGGGTTCACGAGGTG | 59.9 | 0.05 |
| mm4_intron_Inpp4a_chr1_37366459_R | GTCTCGTGGGCTCGGTCAGTAGAAGGACGCTTGGC | 59.7 | 0.05 |
| mm4_intron_Gbp5_chr3_142511227_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intron_Gbp5_chr3_142511227_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_Cdkal1|E2f3_chr13_29863718_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_Cdkal1|E2f3_chr13_29863718_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_Nfyb|1700028I16Rik_chr10_82777904_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_Nfyb|1700028I16Rik_chr10_82777904_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_Gm25423|Gm26054_chr7_116746092_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_Gm25423|Gm26054_chr7_116746092_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intron_Sobp_chr10_43048788_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intron_Sobp_chr10_43048788_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Gm10430/C530008M17Rik|Gm7494_chr5_76667588_F | TCGTCGGCAGCGTCGCTCTGGTTGGTTCTGGGTA | 59.3 | 0.04 |
| mm4_intergenic_Gm10430/C530008M17Rik|Gm7494_chr5_76667588_R | GTCTCGTGGGCTCGGCACAGGACACAGGCACTCAT | 59.9 | 0.04 |
| mm4_intron_Pnn_chr12_59067352_F | TCGTCGGCAGCGTCAGTGCTTGCGAATGGAAGGA | 59.9 | 0.04 |
| mm4_intron_Pnn_chr12_59067352_R | GTCTCGTGGGCTCGGGGGTGGAAAGGGGCTTTGTA | 59.8 | 0.04 |
| mm4_intergenic_Gm25633|Gm8907_chr9_49976316_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Gm25633|Gm8907_chr9_49976316_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_1700011I03Rik|Slc12a2_chr18_57878616_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_1700011I03Rik|Slc12a2_chr18_57878616_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Prickle4|Frs3_chr17_47695150_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Prickle4|Frs3_chr17_47695150_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_exon_Mmadhc_chr2_50296417_F | TCGTCGGCAGCGTCGCAGTATAGGCGTGTCCCTC | 59.9 | 0.03 |
| mm4_exon_Mmadhc_chr2_50296417_R | GTCTCGTGGGCTCGGAGTGCCAGCGCCCTAGTG | 62.0 | 0.03 |
| mm4_intron_Gpc6_chr14_117579856_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intron_Gpc6_chr14_117579856_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intron_Dscam_chr16_97039342_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intron_Dscam_chr16_97039342_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Suz12|Crlf3_chr11_80043438_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Suz12|Crlf3_chr11_80043438_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Ninj2|Gm25327_chr6_120125329_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Ninj2|Gm25327_chr6_120125329_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intron_Dlgap2_chr8_14206230_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intron_Dlgap2_chr8_14206230_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_exon_Gpatch4_chr3_88043511_F | TCGTCGGCAGCGTCAACGATTCTCAAGGGCTGGG | 60.0 | 0.02 |
| mm4_exon_Gpatch4_chr3_88043511_R | GTCTCGTGGGCTCGGGCTGTAAGCCTTCCCCACAT | 60.0 | 0.02 |
| mm4_intergenic_Nsmce2|Trib1_chr15_59636198_F | TCGTCGGCAGCGTCATCAAAGTGGGTTCAGGGCG | 60.6 | 0.02 |
| mm4_intergenic_Nsmce2|Trib1_chr15_59636198_R | GTCTCGTGGGCTCGGCTGTCCCATGGCTTCCTCAG | 60.1 | 0.02 |
| mm4_intron_Set_chr2_30068458_F | TCGTCGGCAGCGTCTGGTGAGCACACTCGTGTTT | 60.1 | 0.02 |
| mm4_intron_Set_chr2_30068458_R | GTCTCGTGGGCTCGGTGCACCTCTGAAAGAGCACC | 60.2 | 0.02 |
| mm4_intron_Taok3_chr5_117215905_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intron_Taok3_chr5_117215905_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Gm24445|Spg7_chr8_123020252_F | TCGTCGGCAGCGTCCCGTCAGGTGAGATGTTCCC | 60.1 | 0.02 |
| mm4_intergenic_Gm24445|Spg7_chr8_123020252_R | GTCTCGTGGGCTCGGCTGATGCAGTGCTGGGTTGT | 60.8 | 0.02 |
| mm4_intron_Pitpnc1_chr11_107299075_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_Pitpnc1_chr11_107299075_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Sec11c|Grp_chr18_65852082_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Sec11c|Grp_chr18_65852082_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Muc6|Muc2_chr7_141694688_F | TCGTCGGCAGCGTCCAGGAACCTGGATGGAGCTG | 60.1 | 0.01 |
| mm4_intergenic_Muc6|Muc2_chr7_141694688_R | GTCTCGTGGGCTCGGGACCCCTCACTTTCCAACCC | 60.2 | 0.01 |
| mm4_intergenic_Pbx1|Pbx1/AC096625.1_chr1_168347783_F | TCGTCGGCAGCGTCCTGGAACACAGGACGGTCAG | 60.3 | 0.01 |
| mm4_intergenic_Pbx1|Pbx1/AC096625.1_chr1_168347783_R | GTCTCGTGGGCTCGGGGCCCATTATCCCTACCTCC | 59.0 | 0.01 |
| mm4_exon_Nbl1_chr4_139083274_F | TCGTCGGCAGCGTCTTCCAAGTCCATCCAGTGCC | 59.9 | 0.01 |
| mm4_exon_Nbl1_chr4_139083274_R | GTCTCGTGGGCTCGGTTCCTCCCCTCTTGTTCCCT | 59.8 | 0.01 |
| mm3_intron_Adrm1_chr2_180171942_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm3_intron_Adrm1_chr2_180171942_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Tbx4|Gm11443_chr11_85916916_F | TCGTCGGCAGCGTCAGATGGTACCCTGCCAGGAT | 60.0 | 0.00 |
| mm4_intergenic_Tbx4|Gm11443_chr11_85916916_R | GTCTCGTGGGCTCGGTACTGAGCCGAGTAGGTGCT | 60.0 | 0.00 |
| mm4_intergenic_Tfap2c|Gm22773_chr2_172709549_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Tfap2c|Gm22773_chr2_172709549_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Pkp1|Igfn1_chr1_135928463_F | TCGTCGGCAGCGTCGCCATGGAGACGTCACTTCT | 59.7 | 0.00 |
| mm4_intergenic_Pkp1|Igfn1_chr1_135928463_R | GTCTCGTGGGCTCGGAGGCTGAGAGACTGGCAGTT | 60.8 | 0.00 |
| mm4_intergenic_Gm22825|Zfhx3_chr8_108099352_F | TCGTCGGCAGCGTCCACCCCTACCCCTCCCAATA | 60.0 | 0.00 |
| mm4_intergenic_Gm22825|Zfhx3_chr8_108099352_R | GTCTCGTGGGCTCGGAGAGCAGCCGTCTGTAAGAC | 59.4 | 0.00 |
| mm4_intergenic_Agpat2|Fam69b_chr2_26609717_F | TCGTCGGCAGCGTCGCCCTGTCTGGTAGGCATTT | 60.0 | 0.00 |
| mm4_intergenic_Agpat2|Fam69b_chr2_26609717_R | GTCTCGTGGGCTCGGAGCTGTTGAAGGCACCAGTT | 60.1 | 0.00 |
| mm4_exon_Zfp711_chrX_112600843_F | TCGTCGGCAGCGTCGAACGCCAGGACCTTCCAG | 60.3 | 0.00 |
| mm4_exon_Zfp711_chrX_112600843_R | GTCTCGTGGGCTCGGGCTCAGGAGTCCCCTTACCT | 60.3 | 0.00 |
| mm3_intron_Itga2b_chr11_102455343_F | TCGTCGGCAGCGTCCAGGAAACTCTGAGGGCAGG | 60.0 | 0.00 |
| mm3_intron_Itga2b_chr11_102455343_R | GTCTCGTGGGCTCGGTTGTGCTGGCCATGTACCAT | 59.9 | 0.00 |
These only list off-targets that have primers in the table above. Primers underlined, off-targets in bold.
| ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52314003 |
AACCGCCAGAGAAGATGGTGCCCCACGGTTGCCTGAGCCCGCGAGCCGGCCCTCCGACTTCCCGGGAGCGTGGCGGGGGA GGCCAGGAGGAGGAGCCGGTCGATGGACTAGCAGGCAG |
| mm4_intron_Frmd4a_chr2_4598953 |
CAGTTTTGCCCAACGTCCTGGGCCTCCGACCTCCTGAGAGCTAGGAGTGCCTCCTCCAATGTCCTGTGTTCCTACTTGCG GT |
| mm4_intergenic_Ubr5|Odf1_chr15_38178298 |
CCAGAAGCAGAAGTCCAGCAGGTCAGGCCTGGTGCTCAGGCTTCCTGGGAGCGTGGAGAAGCCTTAGAGCAAAGGAACGG ACATTTTAGATGATTTTCTCGGGGTGGGG |
| mm4_exon_Gm15917_chr10_81572801 |
AGACACCAAAAGCCGGGAATCAGGAACCGCTGGGGCCTGGGGACAGCTTGTGGGACACTGTTTTCTCAGACTTCCCAGGA GCCAGGTGTGTTCCTCGGTCTGCAAG |
| mm4_exon_Ctbp2_chr7_133123831 |
GTTTTCTCCTCCCGGGAAGGAGGAGGAAAGCGCAGAGAAAAAGGAAAGGGAAGGACGGAAGCGGCGCGGGCCGCTGCTCC GGTGCCCTCCGGCTGCGCAGGAGCGAGGCGGGAGGCGAGGTGAGGGTC |
| mm4_intron_Fam78a_chr2_32073731 |
AGCCTTGACACTCAGCCTTCCTGACCTGCATGGGACGAGACGCTGAGGCCTGAAGTCCGGGCTCTCGGGAAGGCAGAGGC AAGAGACCAAGTGTTCAAGGTCAGAAACCTGGAGGCTGCTTTGA |
| mm4_intergenic_Slc22a16|Ddo_chr10_40629889 | GAGGCAGTTCCACAGAGCTTACACGTGCCTCTCTCCTGGGGAGTCAGAGGCAGACACTGTGGCATGGACCAAT |
| mm4_intergenic_Spata19|Opcml_chr9_27573118 |
AGGGTGTCTGGAACATACAGATTCAGAAAAATATCAACTTCTAGTAGTGGTAGAAAGACTCCCAGCTCCTGGAAAGTCAG AGGGAAACTGNNNNNNNNNNNNNNNNNNNNAGCTGTTCTCTAGTGATGGTCAGTCCTGGA |
| mm4_exon_Chic2_chr5_75044628 |
CAACCACAGCAACAGCCCGAGCCACCCGCAGCAGGAGCAGCCGGCTACCGCATCGCGAGACTTCCCGGGAGCNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCGTTCACGCGGACGGTCTC |
| mm4_intergenic_6030443J06Rik|Gm21846_chr5_22746026 |
CAGGAGGGAGGGAAGAGGGGGGCGGGAGGCTGTGCCTGGCGAGCCGGAGGGGTGCTCCACGCTCCCCCTCCCTCCTTCCG GGAGCGAGGGTGCAGACTCTAGAACTGGTGCTGCTGGG |
| mm4_exon_Txlna_chr4_129640255 |
GAAAAGCAGAGCTGGCACACACCCTCGGTCTTCCCAGGAGCCTGGCTTGTGGAACCTTCGGCTTCCGCCCCAGGAGCTGT CTGTCTGGGTC |
| mm4_intron_Dnajc13_chr9_104236432 |
GTAAAGCCCATGCACGGTTGAGAAATGGGATGCATCATGAGAAAACCCAAAAGTCCTCCATCCCCGCTCCCTGGAAGTCT CTGGTTTAATGGAGAGGCCTGGCTGGGAAAGTCAGGACTGCAC |
| mm3_intergenic_Igdcc3|Gm25313_chr9_65188730 |
AAGTTGGTGCAGGAGAGCAGCAACTGAGGCAGAAGGCTCTCCTCTGACTTCCCTGGAGCCAGGCCTGGCAGTCTGGTAGC CCTGCACTTAAAAAGCTGGACCGCGACATGAGGACTTTTGCTGCCACCTTG |
| mm4_intron_Aplf_chr6_87640140 |
CACGGTCACTCCTGCATCTAGTCTCTTCCTTCCACCCCCACCCCCCCCCCTCTCCCTGGAACTCAGAGGGCCTGTCTACT TGATGCCTCTGGTGGAAAGG |
| mm4_intergenic_Lipc|Aqp9_chr9_70963548 |
AATGAGAGCAGCTTCCGCTCAGGCTGCAGCAGAGGCTTGGCTCCTCCAGCTTCCCAGGAGGGTGGAAAGCTCAAAGCCAG TTCTGGCTAGTGTGGATTAGAGTTCCCAGCAGCCCAGGCACTGTGTCTTGGAGT |
| mm4_intergenic_Tcrg-C1|Tcrg-V3_chr13_19236504 |
AGCTGCACCATTGTCTCCTCCATGTACCCTCTCTCCTGGAAAGTCAGAGGGAGAGGCCTCCAATTGCTGCAGAGTAGTGC CC |
| mm4_intron_Inpp4a_chr1_37366459 |
TGTCATGGGTTCACGAGGTGAGGGTGGGTCGGTTATAGGAACCACCGACTTCCCTGGATTGTGGTACGTGGGTCAGAGGG ACGCCAAGCGTCCTTCTACTGA |
| mm4_intergenic_Gm10430/C530008M17Rik|Gm7494_chr5_76667588 |
GCTCTGGTTGGTTCTGGGTACAGAGAAGCCAAACTGGGCAGGACTTCCCACTTCCCCGGGGCGGGGGAAGGGGGGGGGTG TAAGGGAAATGAGTGCCTGTGTCCTGTG |
| mm4_intron_Pnn_chr12_59067352 |
AGTGCTTGCGAATGGAAGGAAGGCCCGCCCGGCTGCCCGGCGTGGTTTCCGCGCCCCCGGGAAGGCCGAGACCACGGCTT CCCGCCCTTGGCGCTGCAGGCCCGGAACTGCAGTACAAAGCCCCTTTCCACCC |
| mm4_exon_Mmadhc_chr2_50296417 |
GCAGTATAGGCGTGTCCCTCCCCAAAGCCCGGCCTCGGGCCCCGCGTCCCCGGGAAGCCGGGGGACACTAGGGCGCTGGC ACT |
| mm4_exon_Gpatch4_chr3_88043511 |
AACGATTCTCAAGGGCTGGGAAATAAAGCTTGCCTCCGCCTTCCGGGAAGTGTGGTTTTGAATTACTGTGATGAGAAATA GAATGTGGGGAAGGCTTACAGC |
| mm4_intergenic_Nsmce2|Trib1_chr15_59636198 |
ATCAAAGTGGGTTCAGGGCGCATACCTGTAGCCCTCCGTCTTCCTGGGCCCGTGGAGGGATCTCCTGCCAACCTTGTAGT CTGAGGAAGCCATGGGACAG |
| mm4_intron_Set_chr2_30068458 |
TGGTGAGCACACTCGTGTTTCAGTCTGGTTATAACTCCCGGGTTAATGTAATCTTTCCTCAGCCTTCCCAGGTGCGGGGG TACTGTAGGTAGGGTGCTCTTTCAGAGGTGCA |
| mm4_intergenic_Gm24445|Spg7_chr8_123020252 | CCGTCAGGTGAGATGTTCCCTCCCTCCCTCCAGGGAAGTGGGAAGTGGAAAGGCCCACAACCCAGCACTGCATCAG |
| mm4_intergenic_Muc6|Muc2_chr7_141694688 |
CAGGAACCTGGATGGAGCTGGGGACCCTCTGTCTTCCAGGGAGGGTGGAAAAAATGATTCTGGAGAAGGCTGTCCTTGGG GTGGTGGGTAATGCTGTTCTTAGAAATGGGTTGGAAAGTGAGGGGTC |
| mm4_intergenic_Pbx1|Pbx1/AC096625.1_chr1_168347783 |
CTGGAACACAGGACGGTCAGATCACTACAGCACCACCTCCGATTTCTCGGGGGGGGGGGGGGGGATCTGCATGTCAATCG AGACTGGAATATGTATTCCTATATGGAGGTAGGGATAATGGGCC |
| mm4_exon_Nbl1_chr4_139083274 |
TTCCAAGTCCATCCAGTGCCAAAGGGGGAGGGGGGCTTCTGCCTATCCCCTCCCTCTTCCCTGGAGGGTGGGACCGACAT TCCACAGGGAACAAGAGGGGAGGAA |
| mm4_intergenic_Tbx4|Gm11443_chr11_85916916 |
AGATGGTACCCTGCCAGGATGCTNNNNNNNNNNNNNNNNNNNNNTGGAACTCGGAGGGCTGGGTGTGCGTTCCTAGATTC TCTCTAAGCACCTACTCGGCTCAGTA |
| mm4_intergenic_Pkp1|Igfn1_chr1_135928463 |
GCCATGGAGACGTCACTTCTCTACCAGGAGGAATTTATGAAGGCAGTTACACCTCTCTGCCAGGAAGTCAGAGGGGCCTA GCCTCTAATAACTGCCAGTCTCTCAGCCT |
| mm4_intergenic_Gm22825|Zfhx3_chr8_108099352 |
CACCCCTACCCCTCCCAATAGCCACGTTACAGGGAAGTTGGAGGCTCTCCTTCAGTGATGTGTCTTACAGACGGCTGCTC T |
| mm4_intergenic_Agpat2|Fam69b_chr2_26609717 |
GCCCTGTCTGGTAGGCATTTAAGCATGTGGCTGTTGTCCTCCAATCCCCTCCAACTTCCCTGCAGCCTGGTCTGGGACAG ACAGCAGGCTGTTTCCCAACCTCTAGCTATAACTGGTGCCTTCAACAGCT |
| mm4_exon_Zfp711_chrX_112600843 |
GAACGCCAGGACCTTCCAGGTCCACGGCTGCCGCCCCCCGCGGCAGTTGAGAGCGCGGCCTCCGACTGCCTGGCGGCGGG GAAGGCGGTGGCGGTTGCAGCCCCGCGGAGGCGGTGACAGGTAAGGGGACTCCTGAGC |
| mm3_intron_Itga2b_chr11_102455343 |
CAGGAAACTCTGAGGGCAGGTCTCAGTTTCCTCCGACTTCCCGACAGCAGGGGAAGGGATCTGAAGGCCTGAGGATTTCA GACGTCCTGCTTGGGGGGATGGGAGGGATGGTACATGGCCAGCACAA |