CRISPOR

DNA break repair outcome predictions

Guide: TGGGGCACCATCTTCTCTGG CGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

mh- Score	Sequence	Effect
	`CTCGCGGGCTCAGGCAACCGTGGGGCACCATCTTCTCTGGCGGTTCAGGCAGCGGGCTGCTCACGGCTTCGGACAAATTA`	(Wild-type sequence)
344	`CAGGCAACCGTGGGGCACCATCTTCTCTGG---TTCAGGCAGCGGGCTGCTCACGGCTTC`	`3 bp deleted → no frameshift`
255	`CAGGCAACCGTGGGGCACCATCTTCTCTGG---------CAGCGGGCTGCTCACGGCTTC`	`9 bp deleted → no frameshift`
230	`CAGGCAACCGTGGGGCACCATCTTC-----------AGGCAGCGGGCTGCTCACGGCTTC`	`11 bp deleted → frameshift`
210	`CAGGCAACCGTGGGGCA------------------------GCGGGCTGCTCACGGCTTC`	`24 bp deleted → no frameshift`
191	`CAGGCAACCGTGGGGCACCATCTTCTC---------AGGCAGCGGGCTGCTCACGGCTTC`	`9 bp deleted → no frameshift`
188	`CAGGCAACCGTGGGGCACCATCTTCTCTGG---------------GCTGCTCACGGCTTC`	`15 bp deleted → no frameshift`
184	`CAGGCAACCGTGGGGCACCATCTTCTCTG--------------------CTCACGGCTTC`	`20 bp deleted → frameshift`
179	`CAGGCAACCGTGGGGCACCATCTTCTCTGG----------------CTGCTCACGGCTTC`	`16 bp deleted → frameshift`
174	`CAGGCA-----------------------------------GCGGGCTGCTCACGGCTTC`	`35 bp deleted → frameshift`
169	`CAGGCAACCGTGGGGC-------------------------------TGCTCACGGCTTC`	`31 bp deleted → frameshift`
162	`CAGGCAACCGTGGGG------------------TTCAGGCAGCGGGCTGCTCACGGCTTC`	`18 bp deleted → no frameshift`
154	`CAGGCAACCGTGGG-------------------TTCAGGCAGCGGGCTGCTCACGGCTTC`	`19 bp deleted → frameshift`
149	`CAGGCAACCGTGGGGCACCATC--------------AGGCAGCGGGCTGCTCACGGCTTC`	`14 bp deleted → frameshift`
147	`CAGGCAACCGTGG--------------------TTCAGGCAGCGGGCTGCTCACGGCTTC`	`20 bp deleted → frameshift`
143	`CAGGCAACCGTGGGGCACCATCTTCTC-------------------------ACGGCTTC`	`25 bp deleted → frameshift`
133	`CAGGCAACCG----------------------GTTCAGGCAGCGGGCTGCTCACGGCTTC`	`22 bp deleted → frameshift`
128	`CAGGCAACCGTGGGGCACCA-----------------GGCAGCGGGCTGCTCACGGCTTC`	`17 bp deleted → frameshift`
121	`CAGGCAACCGTGGG--------------------------------CTGCTCACGGCTTC`	`32 bp deleted → frameshift`
114	`CAGGCAACCGTGGG-------------------------CAGCGGGCTGCTCACGGCTTC`	`25 bp deleted → frameshift`
110	`CAGGCAACCGTGGGGCA--------------------GGCAGCGGGCTGCTCACGGCTTC`	`20 bp deleted → frameshift`
109	`CAGGCAACCGTGGGGCACCATCTTCTCTGG--------------------------CTTC`	`26 bp deleted → frameshift`
109	`CAGGCAACCGTGG--------------------------CAGCGGGCTGCTCACGGCTTC`	`26 bp deleted → frameshift`
105	`CAGGCAACCGTGGGGCACCA---------------------GCGGGCTGCTCACGGCTTC`	`21 bp deleted → no frameshift`
104	`CAGGCAACCGTGGGGCACCATCTTC-----------------------------------`	`35 bp deleted → frameshift`
103	`CAGGCAACCGTGGGGC---------------------------GGGCTGCTCACGGCTTC`	`27 bp deleted → no frameshift`
99	`CAGGCAACCGTGGGGCACCATCTTCT----------------------GCTCACGGCTTC`	`22 bp deleted → frameshift`
95	`CAGGCAACCGTGGGGCACCATCTTCTCT-----------------------CACGGCTTC`	`23 bp deleted → frameshift`
95	`CAGGCAACCGT-----------------------TCAGGCAGCGGGCTGCTCACGGCTTC`	`23 bp deleted → frameshift`
94	`CAGG-----------------------------TTCAGGCAGCGGGCTGCTCACGGCTTC`	`29 bp deleted → frameshift`
89	`CAGGCAACCGTGGGG------------------------------GCTGCTCACGGCTTC`	`30 bp deleted → no frameshift`
86	`CAGGCAACCGTGGGGCACCATCT-------------------------GCTCACGGCTTC`	`25 bp deleted → frameshift`
82	`CAGGCAACCGTGGGGCAC------------------------------------GGCTTC`	`36 bp deleted → no frameshift`
77	`CAGGCAACCGTGGGGCACCATCTTC---------------------------ACGGCTTC`	`27 bp deleted → no frameshift`
77	`CAGGCAACCGTGGGGC-----------------------------------------TTC`	`41 bp deleted → frameshift`
76	`CAGGCAACCGTGG---------------------------------CTGCTCACGGCTTC`	`33 bp deleted → no frameshift`
74	`CAGGCAACCGTGGGGCACCATCT----------------------------CACGGCTTC`	`28 bp deleted → frameshift`
73	`CAGGCAACCGTGGGGC----------------------------------TCACGGCTTC`	`34 bp deleted → frameshift`
73	`CAGGCAACCG----------------------------------GGCTGCTCACGGCTTC`	`34 bp deleted → frameshift`
73	`CAGGC------------------------------------------TGCTCACGGCTTC`	`42 bp deleted → no frameshift`
71	`CAG---------------------------------------CGGGCTGCTCACGGCTTC`	`39 bp deleted → no frameshift`
66	`CAGGCAACCGTGGGGCACCATCTTCTCT------------------------------TC`	`30 bp deleted → no frameshift`
66	`CAGGCAACCGTGGGGCACCATC------------------------------ACGGCTTC`	`30 bp deleted → no frameshift`
63	`CAGGCA-------------------------------GGCAGCGGGCTGCTCACGGCTTC`	`31 bp deleted → frameshift`
60	`CAGGCAACCGTGGGGCACCATCTTCT--------------------------------TC`	`32 bp deleted → frameshift`
60	`CAGGC--------------------------------------GGGCTGCTCACGGCTTC`	`38 bp deleted → frameshift`
57	`CAGGCAACCGTGGGGCACCATCTTCTC---------------------------------`	`33 bp deleted → no frameshift`
57	`CAGGCAACCGTGGGGCACCA---------------------------------CGGCTTC`	`33 bp deleted → no frameshift`
51	`CAGG-----------------------------------------GCTGCTCACGGCTTC`	`41 bp deleted → frameshift`
48	`CAGGCAACCGTGGG------------------------------------------CTTC`	`42 bp deleted → no frameshift`
47	`CAGGCAACCGTG-------------------------------------CTCACGGCTTC`	`37 bp deleted → frameshift`
46	`CAGGCAACCGTGG-------------------------------------------CTTC`	`43 bp deleted → frameshift`
45	`CAGGCAACCGTGGGGCACCATC--------------------------------------`	`38 bp deleted → frameshift`
44	`CAGGC----------------------------------------------------TTC`	`52 bp deleted → frameshift`
42	`CAGGCAACCG---------------------------------------------GCTTC`	`45 bp deleted → no frameshift`
42	`CAGGC---------------------------------------------TCACGGCTTC`	`45 bp deleted → no frameshift`
30	`CAGGCAAC----------------------------------------------GGCTTC`	`46 bp deleted → frameshift`
28	`CAGGCA-----------------------------------------------CGGCTTC`	`47 bp deleted → frameshift`
23	`CA---------------------------------------------------CGGCTTC`	`51 bp deleted → no frameshift`

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