CRISPOR

DNA break repair outcome predictions

Guide: GAGCCGGTCGATGGACTAGC AGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

mh- Score	Sequence	Effect
	`GCGGGGGAGGCCAGGAGGAGGAGCCGGTCGATGGACTAGCAGGCAGTGCTGCAGGGCTGGGCGCCGAGCCACGGTCTGCT`	(Wild-type sequence)
327	`CCAGGAGGAGGAGCCGGTCGATGGACTAGC----AGTGCTGCAGGGCTGGGCGCCGAGCC`	`4 bp deleted → frameshift`
258	`CCAGGAGGAGGAGCCGGTCGATGGACTAG---GCAGTGCTGCAGGGCTGGGCGCCGAGCC`	`3 bp deleted → no frameshift`
255	`CCAGGAGGAGGAGCCGGTCGATGGACTAGC---------TGCAGGGCTGGGCGCCGAGCC`	`9 bp deleted → no frameshift`
255	`CCAGGAGGAGGAGCCGGTCGATGG---------CAGTGCTGCAGGGCTGGGCGCCGAGCC`	`9 bp deleted → no frameshift`
219	`CCAGGAGGAGGAGCCGGTCGATGGACTAGC------------AGGGCTGGGCGCCGAGCC`	`12 bp deleted → no frameshift`
211	`CCAGGAGGAGGAGCCGGTCGATGGACTAG-------TGCTGCAGGGCTGGGCGCCGAGCC`	`7 bp deleted → frameshift`
179	`CCAGGAGGAGGAGCCGG----------------CAGTGCTGCAGGGCTGGGCGCCGAGCC`	`16 bp deleted → frameshift`
170	`CCAGGAGGAGGAGCCGGTCGATGGACTAGC-----------------TGGGCGCCGAGCC`	`17 bp deleted → frameshift`
166	`CCAGGAGGAGG----------------------CAGTGCTGCAGGGCTGGGCGCCGAGCC`	`22 bp deleted → frameshift`
156	`CCAGGAGGAGGAGCCGGTCGATGGACT-------------GCAGGGCTGGGCGCCGAGCC`	`13 bp deleted → frameshift`
147	`CCAGGAGGAGGAGC--------------------AGTGCTGCAGGGCTGGGCGCCGAGCC`	`20 bp deleted → frameshift`
143	`CCAGGAGG-------------------------CAGTGCTGCAGGGCTGGGCGCCGAGCC`	`25 bp deleted → frameshift`
141	`CCAGGAGGAGGAGCCGGTCGATGGACTAG---------------GGCTGGGCGCCGAGCC`	`15 bp deleted → no frameshift`
141	`CCAGGAGGAGGAGCCGGTCGATG---------------CTGCAGGGCTGGGCGCCGAGCC`	`15 bp deleted → no frameshift`
140	`CCAGGAGGAGGAGCCGGTCGATGG---------------------GCTGGGCGCCGAGCC`	`21 bp deleted → no frameshift`
136	`CCAGGAGGAGGAGCCGGTCGATGG--------------------------GCGCCGAGCC`	`26 bp deleted → frameshift`
133	`CCAGGAGGAGGAGCCGGTCGATGGACTAGC----------------------GCCGAGCC`	`22 bp deleted → frameshift`
133	`CCAGGAGGAGGAGCCGGTCGATGG----------------------CTGGGCGCCGAGCC`	`22 bp deleted → frameshift`
123	`CCAGG----------------------------CAGTGCTGCAGGGCTGGGCGCCGAGCC`	`28 bp deleted → frameshift`
122	`CCAGGAGGAGGAGCCGGTCGATG------------------CAGGGCTGGGCGCCGAGCC`	`18 bp deleted → no frameshift`
120	`CCAGGAGGAGGAGCCGGTCGATGGACTAGC------------------------CGAGCC`	`24 bp deleted → no frameshift`
117	`CCAGGAGGAGGAGCCGGTCGATGGACTAGC-----------------------------C`	`29 bp deleted → frameshift`
116	`CCAGGAGGAGGAGCCGGT-------------------GCTGCAGGGCTGGGCGCCGAGCC`	`19 bp deleted → frameshift`
116	`CCAGGAGGAGGAG-------------------GCAGTGCTGCAGGGCTGGGCGCCGAGCC`	`19 bp deleted → frameshift`
114	`CCAGGAGGAGGAGC-------------------------TGCAGGGCTGGGCGCCGAGCC`	`25 bp deleted → frameshift`
108	`CCAGGAGGAGGAGCCG----------------------------------------AGCC`	`40 bp deleted → frameshift`
105	`CCAGGAGGAGGAGCCGGTCGATGGACT---------------------GGGCGCCGAGCC`	`21 bp deleted → no frameshift`
103	`CCAGGAGGAGGAGCCGGTCGATGG---------------------------CGCCGAGCC`	`27 bp deleted → no frameshift`
101	`CCAG--------------------------------TGCTGCAGGGCTGGGCGCCGAGCC`	`32 bp deleted → frameshift`
98	`CCAGGAGGAGGAGCCGG----------------------------GCTGGGCGCCGAGCC`	`28 bp deleted → frameshift`
98	`CCAGGAGGAGGAGC----------------------------AGGGCTGGGCGCCGAGCC`	`28 bp deleted → frameshift`
95	`CCAGGAGGAGGAG-----------------------TGCTGCAGGGCTGGGCGCCGAGCC`	`23 bp deleted → frameshift`
94	`CCAGGAGGAGGAGCC---------------------------------------------`	`45 bp deleted → no frameshift`
94	`CCAGG----------------------------------------GCTGGGCGCCGAGCC`	`40 bp deleted → frameshift`
94	`CCAGGAGGAGGAGCCGG-----------------------------CTGGGCGCCGAGCC`	`29 bp deleted → frameshift`
91	`CCAGGAGGAGG----------------------------------GCTGGGCGCCGAGCC`	`34 bp deleted → frameshift`
82	`CCAGGAGGAGGAGCCGGTCGA------------------------------------GCC`	`36 bp deleted → no frameshift`
81	`CCAGGAGGAG--------------------------TGCTGCAGGGCTGGGCGCCGAGCC`	`26 bp deleted → frameshift`
78	`CCAGGAGG-------------------------------------GCTGGGCGCCGAGCC`	`37 bp deleted → frameshift`
76	`CCAGGAGGAGGAGCCGGTCG---------------------------------CCGAGCC`	`33 bp deleted → no frameshift`
76	`CCAGGAGGAGGAGCCGG---------------------------------GCGCCGAGCC`	`33 bp deleted → no frameshift`
76	`CCAGGAGGAGGAGC---------------------------------TGGGCGCCGAGCC`	`33 bp deleted → no frameshift`
73	`CCAGGAGGAGGAGCCGG----------------------------------CGCCGAGCC`	`34 bp deleted → frameshift`
70	`CCAGGAG-----------------------------TGCTGCAGGGCTGGGCGCCGAGCC`	`29 bp deleted → frameshift`
69	`CCAGGAGGAGG-----------------------------------CTGGGCGCCGAGCC`	`35 bp deleted → frameshift`
63	`CCAGGAGGAGGAG-------------------------------GGCTGGGCGCCGAGCC`	`31 bp deleted → frameshift`
62	`CCAGGAGGAGGAGCCG-------------------------------------CCGAGCC`	`37 bp deleted → frameshift`
60	`CCAGGAGGAGGAGCCGGTCGATGGA--------------------------------GCC`	`32 bp deleted → frameshift`
60	`CCAGGAGGAGGAGC--------------------------------------GCCGAGCC`	`38 bp deleted → frameshift`
60	`CCAGGAGG--------------------------------------CTGGGCGCCGAGCC`	`38 bp deleted → frameshift`
56	`CCAGGAGGAGG---------------------------------------GCGCCGAGCC`	`39 bp deleted → no frameshift`
54	`CCAGGAGGAGG----------------------------------------CGCCGAGCC`	`40 bp deleted → frameshift`
51	`CCAGG-----------------------------------------CTGGGCGCCGAGCC`	`41 bp deleted → frameshift`
48	`CCAGGAGG------------------------------------------GCGCCGAGCC`	`42 bp deleted → no frameshift`
46	`CCAGGAGG-------------------------------------------CGCCGAGCC`	`43 bp deleted → frameshift`
45	`CCAGGAGGAG------------------------------------------------CC`	`48 bp deleted → no frameshift`
42	`CCAGG---------------------------------------------GCGCCGAGCC`	`45 bp deleted → no frameshift`
40	`CCAGG----------------------------------------------CGCCGAGCC`	`46 bp deleted → frameshift`
39	`CCAGGAG---------------------------------------------------CC`	`51 bp deleted → no frameshift`
28	`CC-----------------------------------------------------GAGCC`	`53 bp deleted → frameshift`
22	`CC----------------------------------------------------------`	`58 bp deleted → frameshift`
20	`CCAG------------------------------------------------------CC`	`54 bp deleted → no frameshift`

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