CRISPOR

DNA break repair outcome predictions

Guide: TCCGGGGATGGAGAGCCGAA AGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

mh- Score	Sequence	Effect
	`CCCTCCCCCCCTCTGGAGAGTCCGGGGATGGAGAGCCGAAAGGACATGGTTATGTTTCTGGATGGGGGTCAGCTTGGCAC`	(Wild-type sequence)
274	`CTCTGGAGAGTCCGGGGATGGA------------CATGGTTATGTTTCTGGATGGGGGTC`	`12 bp deleted → no frameshift`
244	`CTCTGGAGAGTCCGGGGATGG------------------TTATGTTTCTGGATGGGGGTC`	`18 bp deleted → no frameshift`
233	`CTCTGGAGAGTCCGGGGATGGAGAGCCGA-----CATGGTTATGTTTCTGGATGGGGGTC`	`5 bp deleted → frameshift`
224	`CTCTGGAGAGTCCGGGGA----------------CATGGTTATGTTTCTGGATGGGGGTC`	`16 bp deleted → frameshift`
211	`CTCTGGAGAGTCCGGGGATGGAGAG-------GACATGGTTATGTTTCTGGATGGGGGTC`	`7 bp deleted → frameshift`
191	`CTCTGGAGAGTCCGGGGATGGAG---------GACATGGTTATGTTTCTGGATGGGGGTC`	`9 bp deleted → no frameshift`
183	`CTCTGGAGAGTCCGGGGATGG----------------------------------GGGTC`	`34 bp deleted → frameshift`
182	`CTCTGGAGAGTCCGGGGATGGAGA----------CATGGTTATGTTTCTGGATGGGGGTC`	`10 bp deleted → frameshift`
170	`CTCTGGAGAGTCCGGG-----------------ACATGGTTATGTTTCTGGATGGGGGTC`	`17 bp deleted → frameshift`
162	`CTCTGGAGAGTCCGG------------------ACATGGTTATGTTTCTGGATGGGGGTC`	`18 bp deleted → no frameshift`
133	`CTCTGGAGAGTCCGGGGATGGA------------------------------TGGGGGTC`	`30 bp deleted → no frameshift`
133	`CTCTGGAGAGTCCGGGG----------------------TTATGTTTCTGGATGGGGGTC`	`22 bp deleted → frameshift`
129	`CTCTGGA---------------------------CATGGTTATGTTTCTGGATGGGGGTC`	`27 bp deleted → no frameshift`
126	`CTCTGGAGAGTCCGGG-----------------------TTATGTTTCTGGATGGGGGTC`	`23 bp deleted → frameshift`
120	`CTCTGGAGAGTCCGGGGATG------------------------TTTCTGGATGGGGGTC`	`24 bp deleted → no frameshift`
120	`CTCTGGAGAGTCCGG------------------------TTATGTTTCTGGATGGGGGTC`	`24 bp deleted → no frameshift`
108	`CTCTGGAGAGTCCGGGG----------------------------------------GTC`	`40 bp deleted → frameshift`
103	`CTCTGGAGAGTCCGGGG-----------------------------------------TC`	`41 bp deleted → frameshift`
99	`CTCTGGAGAG----------------------GACATGGTTATGTTTCTGGATGGGGGTC`	`22 bp deleted → frameshift`
96	`CTCTGG---------------------------------TTATGTTTCTGGATGGGGGTC`	`33 bp deleted → no frameshift`
95	`CTCTGGAGAGTCCGGGGATGGAGAGCCGA-----------------------TGGGGGTC`	`23 bp deleted → frameshift`
94	`CTCTGGA---------------------------------------------TGGGGGTC`	`45 bp deleted → no frameshift`
90	`CTCTGGAG------------------------GACATGGTTATGTTTCTGGATGGGGGTC`	`24 bp deleted → no frameshift`
86	`CTCTGGAGA-------------------------CATGGTTATGTTTCTGGATGGGGGTC`	`25 bp deleted → frameshift`
85	`CTCTGGAGAGTCCGGGG---------------------------------------GGTC`	`39 bp deleted → no frameshift`
74	`CTCTGGAGAGTCCGGGGATGGAGA----------------------------TGGGGGTC`	`28 bp deleted → frameshift`
73	`CTCTGGAGAGTCCGGG------------------------------------------TC`	`42 bp deleted → no frameshift`
70	`CTCTGGAGAGT-----------------------------TATGTTTCTGGATGGGGGTC`	`29 bp deleted → frameshift`
69	`CTCTGGAGAGTCCGGGGATGG-----------------------------------GGTC`	`35 bp deleted → frameshift`
69	`CTCTGGAGAGTCCGGG-----------------------------------ATGGGGGTC`	`35 bp deleted → frameshift`
66	`CTCTGGAGAGTCCGGGGATGG------------------------------------GTC`	`36 bp deleted → no frameshift`
66	`CTCTGGAGAGTCCGG------------------------------------ATGGGGGTC`	`36 bp deleted → no frameshift`
62	`CTCTGGAGAGTCCGGGGATGG-------------------------------------TC`	`37 bp deleted → frameshift`
60	`CTCTGGAGAGTCCGGGG--------------------------------------GGGTC`	`38 bp deleted → frameshift`
54	`CTCTGGAGAGT----------------------------------TTCTGGATGGGGGTC`	`34 bp deleted → frameshift`
49	`CTCTGGAGAGTC------------------------------------TGGATGGGGGTC`	`36 bp deleted → no frameshift`
46	`CTCTGGAGAGTCCGG-------------------------------------------TC`	`43 bp deleted → frameshift`
45	`CTCTGGAGAGTC------------------------------------------------`	`48 bp deleted → no frameshift`
43	`CTCTGG-------------------------------------------------GGGTC`	`49 bp deleted → frameshift`
42	`CTCTG---------------------------------------TTTCTGGATGGGGGTC`	`39 bp deleted → no frameshift`
34	`CTCTGGAGA-------------------------------------------TGGGGGTC`	`43 bp deleted → frameshift`
32	`CTCTGG--------------------------------------------------GGTC`	`50 bp deleted → frameshift`
31	`CTCTGG---------------------------------------------------GTC`	`51 bp deleted → no frameshift`
29	`CTCTGG----------------------------------------------------TC`	`52 bp deleted → frameshift`
28	`CT-----------------------------------------------GGATGGGGGTC`	`47 bp deleted → frameshift`
17	`CTC---------------------------------------------------------`	`57 bp deleted → no frameshift`

Version 5.01 - Documentation - Contact us - Downloads/local installation - Citation - License