CRISPOR

DNA break repair outcome predictions

Guide: CTCGCGGGCTCAGGCAACCG TGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

mh- Score	Sequence	Effect
	`CCGGGAAGTCGGAGGGCCGGCTCGCGGGCTCAGGCAACCGTGGGGCACCATCTTCTCTGGCGGTTCAGGCAGCGGGCTGC`	(Wild-type sequence)
403	`GGAGGGCCGGCTCGCGGGCTCAGGCA-----------CCATCTTCTCTGGCGGTTCAGGC`	`11 bp deleted → frameshift`
341	`GGAGGGCCGGCTCGCGGGC-----------------ACCATCTTCTCTGGCGGTTCAGGC`	`17 bp deleted → frameshift`
303	`GGAGGGCCGGCTCGCGGGCTCAGGCAACC----------ATCTTCTCTGGCGGTTCAGGC`	`10 bp deleted → frameshift`
269	`GGAGGGCCGGCTCGCGGG----------------GCACCATCTTCTCTGGCGGTTCAGGC`	`16 bp deleted → frameshift`
255	`GGAGGGCCGGCTCGCGGGCTCAGG---------GGCACCATCTTCTCTGGCGGTTCAGGC`	`9 bp deleted → no frameshift`
242	`GGAGGGCCGGCTCGCGGGCTCAGG----------GCACCATCTTCTCTGGCGGTTCAGGC`	`10 bp deleted → frameshift`
188	`GGAGGGCCGGCTCGCGGG---------------GGCACCATCTTCTCTGGCGGTTCAGGC`	`15 bp deleted → no frameshift`
188	`GGAGGGC-----------------------------ACCATCTTCTCTGGCGGTTCAGGC`	`29 bp deleted → frameshift`
174	`GGAGGGCCGGCTCGCGGGCTCAGGC-----------------------------------`	`35 bp deleted → frameshift`
172	`GGAGGGCCGGC-------------------------ACCATCTTCTCTGGCGGTTCAGGC`	`25 bp deleted → frameshift`
163	`GGAGGGCCGGCTCGCGGGCTCAGGC--------------------------GGTTCAGGC`	`26 bp deleted → frameshift`
162	`GGAGGGCCGGCTCGCGG------------------CACCATCTTCTCTGGCGGTTCAGGC`	`18 bp deleted → no frameshift`
149	`GGAGGGCCGGCTCGCGGGCTCAGGCA--------------TCTTCTCTGGCGGTTCAGGC`	`14 bp deleted → frameshift`
148	`GGAGGG----------------------------GCACCATCTTCTCTGGCGGTTCAGGC`	`28 bp deleted → frameshift`
141	`GGAGGGCCGGCTCGCGGGCTCA---------------CCATCTTCTCTGGCGGTTCAGGC`	`15 bp deleted → no frameshift`
140	`GGAGGGCCGGCTCGC---------------------ACCATCTTCTCTGGCGGTTCAGGC`	`21 bp deleted → no frameshift`
136	`GGAGGGCCGGCTCGCGGGCTC--------------------------TGGCGGTTCAGGC`	`26 bp deleted → frameshift`
133	`GGAGGGCCGGCTCGCGGGCTCAGGCAACCG----------------------GTTCAGGC`	`22 bp deleted → frameshift`
132	`GGAGGGCCGGCTCGCGG------------------------------------TTCAGGC`	`36 bp deleted → no frameshift`
126	`GGAGGGCCGG-----------------------GGCACCATCTTCTCTGGCGGTTCAGGC`	`23 bp deleted → frameshift`
122	`GGAGGGCCGGCTCGCGGGCTCA------------------TCTTCTCTGGCGGTTCAGGC`	`18 bp deleted → no frameshift`
121	`GGAGGGCCGGCTCGCGGGC--------------------------------GGTTCAGGC`	`32 bp deleted → frameshift`
120	`GGAGGGCCGG------------------------GCACCATCTTCTCTGGCGGTTCAGGC`	`24 bp deleted → no frameshift`
105	`GGAGGGCCGGCTCGCGGGCTC---------------------TTCTCTGGCGGTTCAGGC`	`21 bp deleted → no frameshift`
103	`GGAGGG---------------------------GGCACCATCTTCTCTGGCGGTTCAGGC`	`27 bp deleted → no frameshift`
95	`GGAGGGCCGGCTCGCGGGCT-----------------------TCTCTGGCGGTTCAGGC`	`23 bp deleted → frameshift`
94	`GGAGGGCCGGCTCGCGGGCTCAGG-----------------------------TTCAGGC`	`29 bp deleted → frameshift`
91	`GGAGGGCCGGCTC----------------------------------TGGCGGTTCAGGC`	`34 bp deleted → frameshift`
90	`GGAGGGCCGGCTCGCGGGCTC------------------------TCTGGCGGTTCAGGC`	`24 bp deleted → no frameshift`
89	`GGAGG------------------------------CACCATCTTCTCTGGCGGTTCAGGC`	`30 bp deleted → no frameshift`
84	`GGAGGGCC-------------------------------ATCTTCTCTGGCGGTTCAGGC`	`31 bp deleted → frameshift`
84	`GG-------------------------------GGCACCATCTTCTCTGGCGGTTCAGGC`	`31 bp deleted → frameshift`
81	`GGAGGGCCGGC----------------------------------------GGTTCAGGC`	`40 bp deleted → frameshift`
80	`GG--------------------------------GCACCATCTTCTCTGGCGGTTCAGGC`	`32 bp deleted → frameshift`
77	`GGAGGGCCGGCTCGCGGGC-----------------------------------------`	`41 bp deleted → frameshift`
76	`GGAGGGCCGGCTCGCGG---------------------------------CGGTTCAGGC`	`33 bp deleted → no frameshift`
76	`GG---------------------------------CACCATCTTCTCTGGCGGTTCAGGC`	`33 bp deleted → no frameshift`
74	`GGAGGGCCGGCTCGCGGGCT----------------------------GGCGGTTCAGGC`	`28 bp deleted → frameshift`
70	`GGAGGGCCGGCTC-----------------------------TTCTCTGGCGGTTCAGGC`	`29 bp deleted → frameshift`
69	`GGAGGGCCGG-------------------------------------------TTCAGGC`	`43 bp deleted → frameshift`
69	`GGAGGGCCGGCTCGCGGG-----------------------------------TTCAGGC`	`35 bp deleted → frameshift`
66	`GGAGGGC--------------------------------------------GGTTCAGGC`	`44 bp deleted → frameshift`
63	`GGAGGGCCGGCTCGCGGGCTCAGGCA-------------------------------GGC`	`31 bp deleted → frameshift`
63	`GGAGGGCCGGCT-------------------------------TCTCTGGCGGTTCAGGC`	`31 bp deleted → frameshift`
60	`GGAGGGCCGGCTC--------------------------------TCTGGCGGTTCAGGC`	`32 bp deleted → frameshift`
60	`GGAGGGCCGGCTCG--------------------------------------GTTCAGGC`	`38 bp deleted → frameshift`
51	`GGAGGGCCGGC-------------------------------------------------`	`49 bp deleted → frameshift`
49	`GGAGGGCCGGCT------------------------------------GGCGGTTCAGGC`	`36 bp deleted → no frameshift`
48	`GGAGGGCCGGCTCGCGG------------------------------------------C`	`42 bp deleted → no frameshift`
42	`GGAGGGC-----------------------------------------------------`	`53 bp deleted → frameshift`
42	`GGAGGGCCGGCTCGC---------------------------------------------`	`45 bp deleted → no frameshift`
42	`GGAGG---------------------------------------------CGGTTCAGGC`	`45 bp deleted → no frameshift`
38	`GGAGGG-----------------------------------------------TTCAGGC`	`47 bp deleted → frameshift`
36	`GGAGG------------------------------------------------TTCAGGC`	`48 bp deleted → no frameshift`
36	`GG------------------------------------------------CGGTTCAGGC`	`48 bp deleted → no frameshift`
34	`GGAGGGCCGGCTC-------------------------------------------AGGC`	`43 bp deleted → frameshift`
33	`GGAGG------------------------------------------------------C`	`54 bp deleted → no frameshift`
31	`GG---------------------------------------------------TTCAGGC`	`51 bp deleted → no frameshift`
23	`GG---------------------------------------------------------C`	`57 bp deleted → no frameshift`

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