CRISPOR

DNA break repair outcome predictions

Guide: ATGGGGCTTCCTCCCAGAGG CGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

mh- Score	Sequence	Effect
	`AGCTTAGAAAGAAGAGGGCAATGGGGCTTCCTCCCAGAGGCGGTGCGGCACAGAGGAGCGCTCGCATCACAAGGTGACCC`	(Wild-type sequence)
449	`GAAGAGGGCAATGGGGCTTCCTCCCAGAGG----------------AGCGCTCGCATCAC`	`16 bp deleted → frameshift`
344	`GAAGAGGGCAATGGGGCTTCCTCCCAGAGG---TGCGGCACAGAGGAGCGCTCGCATCAC`	`3 bp deleted → no frameshift`
268	`GAAGAGGGCAATGGGGCTTCCTCCCAGAGG--------CACAGAGGAGCGCTCGCATCAC`	`8 bp deleted → frameshift`
199	`GAAGAGGGCAATGGGGC----------------------ACAGAGGAGCGCTCGCATCAC`	`22 bp deleted → frameshift`
193	`GAAGAGGGCAATGGGGCTTCCTCCCAGAG-------------------CGCTCGCATCAC`	`19 bp deleted → frameshift`
170	`GAAGAGGGCAATGGGG-----------------TGCGGCACAGAGGAGCGCTCGCATCAC`	`17 bp deleted → frameshift`
162	`GAAGAGGGCAATGGG------------------TGCGGCACAGAGGAGCGCTCGCATCAC`	`18 bp deleted → no frameshift`
156	`GAAGAGGGCA------------------------------CAGAGGAGCGCTCGCATCAC`	`30 bp deleted → no frameshift`
154	`GAAGAGGGCAATGGGGC-------------------GGCACAGAGGAGCGCTCGCATCAC`	`19 bp deleted → frameshift`
154	`GAAGAGGGCAATGG-------------------TGCGGCACAGAGGAGCGCTCGCATCAC`	`19 bp deleted → frameshift`
149	`GAAGAGGGCAATGGGGCTTCCTCCCAGAG--------------AGGAGCGCTCGCATCAC`	`14 bp deleted → frameshift`
149	`GAAGAGGGCAATGGGGCTTCCTCCCA--------------CAGAGGAGCGCTCGCATCAC`	`14 bp deleted → frameshift`
126	`GAAGAGGGCAATGGG-----------------------CACAGAGGAGCGCTCGCATCAC`	`23 bp deleted → frameshift`
122	`GAAGAGGGCAATGGGGCTTCCTCCCAG------------------GAGCGCTCGCATCAC`	`18 bp deleted → no frameshift`
120	`GAAGAGGGCAATGG------------------------CACAGAGGAGCGCTCGCATCAC`	`24 bp deleted → no frameshift`
114	`GAAGAGGG-------------------------TGCGGCACAGAGGAGCGCTCGCATCAC`	`25 bp deleted → frameshift`
113	`GAAGAGG---------------------------------------AGCGCTCGCATCAC`	`39 bp deleted → no frameshift`
111	`GAAGAGGGCAATGGGGCTTCCTC------------------------------GCATCAC`	`30 bp deleted → no frameshift`
109	`GAAGAGG--------------------------TGCGGCACAGAGGAGCGCTCGCATCAC`	`26 bp deleted → frameshift`
105	`GAAGAGGGCAATGGGGCTTCCTCCCAG---------------------CGCTCGCATCAC`	`21 bp deleted → no frameshift`
103	`GAAGAGGGC---------------------------GGCACAGAGGAGCGCTCGCATCAC`	`27 bp deleted → no frameshift`
99	`GAAGAGGGCAATG----------------------CGGCACAGAGGAGCGCTCGCATCAC`	`22 bp deleted → frameshift`
91	`GAAGAGGGCAATGGGGCT----------------------------------CGCATCAC`	`34 bp deleted → frameshift`
89	`GAAGAGGGCAATGGGG------------------------------AGCGCTCGCATCAC`	`30 bp deleted → no frameshift`
84	`GAAGAGGGCAATGGG-------------------------------AGCGCTCGCATCAC`	`31 bp deleted → frameshift`
84	`GAAGAGG-------------------------------CACAGAGGAGCGCTCGCATCAC`	`31 bp deleted → frameshift`
80	`GAAGAGGGCAATGGGGC--------------------------------GCTCGCATCAC`	`32 bp deleted → frameshift`
80	`GAAGAGGGCAATGG--------------------------------AGCGCTCGCATCAC`	`32 bp deleted → frameshift`
66	`GAAGAGGGCAATGGGGCTTCCTCCCA------------------------------TCAC`	`30 bp deleted → no frameshift`
61	`GAAGAG------------------------------------------CGCTCGCATCAC`	`42 bp deleted → no frameshift`
60	`GAAGAGGGCA--------------------------------GAGGAGCGCTCGCATCAC`	`32 bp deleted → frameshift`
60	`GAAGAGGGCAATGGGGC--------------------------------------ATCAC`	`38 bp deleted → frameshift`
60	`GAAGAGGG--------------------------------------AGCGCTCGCATCAC`	`38 bp deleted → frameshift`
57	`GAAGAGGGCAATGGGGCTTCCTCCCA---------------------------------C`	`33 bp deleted → no frameshift`
57	`GAAGAGGGCAATGGGGCTTC---------------------------------GCATCAC`	`33 bp deleted → no frameshift`
54	`GAAGAGGGC----------------------------------------GCTCGCATCAC`	`40 bp deleted → frameshift`
52	`GAAGAGGGCAATGGGGCTTCCTC-----------------------------------AC`	`35 bp deleted → frameshift`
50	`GAAGAGGGCA----------------------------------------------TCAC`	`46 bp deleted → frameshift`
48	`GAAGAGGGC------------------------------------------TCGCATCAC`	`42 bp deleted → no frameshift`
47	`GAAGAG-------------------------------------AGGAGCGCTCGCATCAC`	`37 bp deleted → frameshift`
45	`GAAGAGGGCAATGGGGCTTC--------------------------------------AC`	`38 bp deleted → frameshift`
38	`GAAG-----------------------------------------GAGCGCTCGCATCAC`	`41 bp deleted → frameshift`
36	`GA------------------------------------------GGAGCGCTCGCATCAC`	`42 bp deleted → no frameshift`
33	`GAAG--------------------------------------------CGCTCGCATCAC`	`44 bp deleted → frameshift`
31	`GA---------------------------------------------GCGCTCGCATCAC`	`45 bp deleted → no frameshift`
25	`GAAGAGGGCA-------------------------------------------------C`	`49 bp deleted → frameshift`
21	`GAAGAGGGCAAT---------------------------------------------CAC`	`45 bp deleted → no frameshift`

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