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The following table lists possible deletions.
Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.
| mh- Score | Sequence | Effect |
|---|---|---|
| GCAACCGTGGGGCACCATCTTCTCTGGCGGTTCAGGCAGCGGGCTGCTCACGGCTTCGGACAAATTAGAGACCCTCTTAC | (Wild-type sequence) | |
| 423 | GGCACCATCTTCTCTGGCGGTTCAGGC-------TGCTCACGGCTTCGGACAAATTAGAG | 7 bp deleted → frameshift |
| 327 | GGCACCATCTTCTCTGGCGGTTCAGGCAGC----TGCTCACGGCTTCGGACAAATTAGAG | 4 bp deleted → frameshift |
| 296 | GGCACCATCTTCTCTGGCGGTTCAGG------GCTGCTCACGGCTTCGGACAAATTAGAG | 6 bp deleted → no frameshift |
| 282 | GGCACCATCTTCTCTGGCGGTTCAGGCAGC-------TCACGGCTTCGGACAAATTAGAG | 7 bp deleted → frameshift |
| 269 | GGCACCATCTTCTCTGGC----------------TGCTCACGGCTTCGGACAAATTAGAG | 16 bp deleted → frameshift |
| 256 | GGCACCATCTTCTCTGGCGGTTCAGGC-----------------TTCGGACAAATTAGAG | 17 bp deleted → frameshift |
| 242 | GGCACCATCTTCTCTGGCGGTTCAGGC----------TCACGGCTTCGGACAAATTAGAG | 10 bp deleted → frameshift |
| 219 | GGCACCATCTTCTCTGGCGG------------GCTGCTCACGGCTTCGGACAAATTAGAG | 12 bp deleted → no frameshift |
| 208 | GGCACCATCTTCTCTGGCGG-------------CTGCTCACGGCTTCGGACAAATTAGAG | 13 bp deleted → frameshift |
| 198 | GGCACCATCTTCTCTGGCGGTTCAGGCAGC--------------TTCGGACAAATTAGAG | 14 bp deleted → frameshift |
| 190 | GGCACCATCTTCTCTGGCGG-----------------------CTTCGGACAAATTAGAG | 23 bp deleted → frameshift |
| 188 | GGCACCATCTTCTCTGG---------------GCTGCTCACGGCTTCGGACAAATTAGAG | 15 bp deleted → no frameshift |
| 184 | GGCACCATCTTCTCTG--------------------CTCACGGCTTCGGACAAATTAGAG | 20 bp deleted → frameshift |
| 179 | GGCACCATCTTCTCTGGCGGTTCA----------------CGGCTTCGGACAAATTAGAG | 16 bp deleted → frameshift |
| 164 | GGCACCATCTTCTCTGGCGGTTCAGGCA------------CGGCTTCGGACAAATTAGAG | 12 bp deleted → no frameshift |
| 163 | GGCACCATCTTCTCTGGC--------------------------TTCGGACAAATTAGAG | 26 bp deleted → frameshift |
| 154 | GGCACCATCTTCTCTGGC-------------------TCACGGCTTCGGACAAATTAGAG | 19 bp deleted → frameshift |
| 143 | GGCACCATCTTCTC-------------------------ACGGCTTCGGACAAATTAGAG | 25 bp deleted → frameshift |
| 141 | GGCACCATCTTCTCTGGCGG-----------------------------ACAAATTAGAG | 29 bp deleted → frameshift |
| 127 | GGC-------------------------------TGCTCACGGCTTCGGACAAATTAGAG | 31 bp deleted → frameshift |
| 126 | GGCACCATCTTCTCTGGCGGTTCAGG-----------------------ACAAATTAGAG | 23 bp deleted → frameshift |
| 120 | GGCACCATCTTCTCTGGCGGTTC------------------------GGACAAATTAGAG | 24 bp deleted → no frameshift |
| 104 | GGCACCATCTTC-----------------------------------GGACAAATTAGAG | 35 bp deleted → frameshift |
| 99 | GGCACCATCTTCT----------------------GCTCACGGCTTCGGACAAATTAGAG | 22 bp deleted → frameshift |
| 95 | GGCACCATCTTCTCT-----------------------CACGGCTTCGGACAAATTAGAG | 23 bp deleted → frameshift |
| 90 | GGCACCATCTTCTCTGGCGGTTCAGGCA------------------------AATTAGAG | 24 bp deleted → no frameshift |
| 89 | GG------------------------------GCTGCTCACGGCTTCGGACAAATTAGAG | 30 bp deleted → no frameshift |
| 86 | GGCACCATCT-------------------------GCTCACGGCTTCGGACAAATTAGAG | 25 bp deleted → frameshift |
| 82 | GGCAC------------------------------------GGCTTCGGACAAATTAGAG | 36 bp deleted → no frameshift |
| 80 | GGCACCATCTTCTCTGG--------------------------------ACAAATTAGAG | 32 bp deleted → frameshift |
| 77 | GGCACCATCTTC---------------------------ACGGCTTCGGACAAATTAGAG | 27 bp deleted → no frameshift |
| 77 | GGC-----------------------------------------TTCGGACAAATTAGAG | 41 bp deleted → frameshift |
| 74 | GGCACCATCTTCTCTGGCGGTTCA----------------------------AATTAGAG | 28 bp deleted → frameshift |
| 74 | GGCACCATCT----------------------------CACGGCTTCGGACAAATTAGAG | 28 bp deleted → frameshift |
| 73 | GGC----------------------------------TCACGGCTTCGGACAAATTAGAG | 34 bp deleted → frameshift |
| 70 | GGCACCATCTTCTCTGGCGGTTCAGGCAG-----------------------------AG | 29 bp deleted → frameshift |
| 66 | GGCACCATCTTCTCT------------------------------TCGGACAAATTAGAG | 30 bp deleted → no frameshift |
| 66 | GGCACCATC------------------------------ACGGCTTCGGACAAATTAGAG | 30 bp deleted → no frameshift |
| 63 | GGCACCATCTTCTCTGGCGGTTCAGGCAG------------------------------- | 31 bp deleted → frameshift |
| 60 | GGCACCATCTTCT--------------------------------TCGGACAAATTAGAG | 32 bp deleted → frameshift |
| 57 | GGCACCATCTTCTCTGGCGGTTCAG---------------------------------AG | 33 bp deleted → no frameshift |
| 57 | GGCACCATCTTCTC---------------------------------GGACAAATTAGAG | 33 bp deleted → no frameshift |
| 57 | GGCACCA---------------------------------CGGCTTCGGACAAATTAGAG | 33 bp deleted → no frameshift |
| 52 | GGCACCATCTTCTCTGGCGGTTCAG----------------------------------- | 35 bp deleted → frameshift |
| 45 | GGCACCATC--------------------------------------GGACAAATTAGAG | 38 bp deleted → frameshift |
| 38 | GG-----------------------------------------------ACAAATTAGAG | 47 bp deleted → frameshift |
| 36 | GGCACCATCTTCTCTGGCGGTT----------------------------------AGAG | 34 bp deleted → frameshift |
| 31 | GGCACCA---------------------------------------------AATTAGAG | 45 bp deleted → no frameshift |
| 30 | GGCAC----------------------------------------------AAATTAGAG | 46 bp deleted → frameshift |
| 27 | GGCA------------------------------------------------AATTAGAG | 48 bp deleted → no frameshift |
| 21 | GGCACCATCTT---------------------------------------------AGAG | 45 bp deleted → no frameshift |
| 19 | GGCACCAT-----------------------------------------------TAGAG | 47 bp deleted → frameshift |