CRISPOR

DNA break repair outcome predictions

Guide: GGTCGCCGTCGACACGGCGC TGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

mh- Score	Sequence	Effect
	`CTCCCCACTGCCATCTCCGCGGTCGCCGTCGACACGGCGCTGGGGCTACCCGGCGCCTGCCTTGTCGCCTTAGCTCCTCT`	(Wild-type sequence)
538	`CCATCTCCGCGGTCGCCGTCGACACGGCGC----------------CTGCCTTGTCGCCT`	`16 bp deleted → frameshift`
402	`CCATCTCCGCGGTCGCCGTCGACACGGC--------TACCCGGCGCCTGCCTTGTCGCCT`	`8 bp deleted → frameshift`
296	`CCATCTCCGCGGTCGCCGTCGACACGGCGC------TACCCGGCGCCTGCCTTGTCGCCT`	`6 bp deleted → no frameshift`
296	`CCATCTCCGCGGTCGCCGTCGACACGG------GGCTACCCGGCGCCTGCCTTGTCGCCT`	`6 bp deleted → no frameshift`
282	`CCATCTCCGCGGTCGCCGTCGACACGG-------GCTACCCGGCGCCTGCCTTGTCGCCT`	`7 bp deleted → frameshift`
208	`CCATCTCCGCGGTCGCCGTCGACACGGCG-------------GCGCCTGCCTTGTCGCCT`	`13 bp deleted → frameshift`
198	`CCATCTCCGCGGTCGCCGTCGACACGGCGC--------------GCCTGCCTTGTCGCCT`	`14 bp deleted → frameshift`
180	`CCATCTCCGCGGTCGCCG------------------------GCGCCTGCCTTGTCGCCT`	`24 bp deleted → no frameshift`
178	`CCATCTCCGCGGTCGCC------------------------------TGCCTTGTCGCCT`	`30 bp deleted → no frameshift`
162	`CCATCTCCGCGGTCGCCGTCGACACGGC------------------CTGCCTTGTCGCCT`	`18 bp deleted → no frameshift`
154	`CCATCTCCGCGGTCGCCGTCGACACG-------------------CCTGCCTTGTCGCCT`	`19 bp deleted → frameshift`
149	`CCATCTCCGCGGTCGCCGTCGACAC--------------CCGGCGCCTGCCTTGTCGCCT`	`14 bp deleted → frameshift`
148	`CCATCTCCGCGGTCGCCGTCGACACGGCGC----------------------------CT`	`28 bp deleted → frameshift`
147	`CCATCTCCGCGGTCGCCGTCGACACGGCGC--------------------CTTGTCGCCT`	`20 bp deleted → frameshift`
147	`CCATCTCCGCGGTCGC--------------------TACCCGGCGCCTGCCTTGTCGCCT`	`20 bp deleted → frameshift`
140	`CCATCTCCGCGGTCGCCGTCG---------------------GCGCCTGCCTTGTCGCCT`	`21 bp deleted → no frameshift`
140	`CCATCTCCGCGG---------------------GGCTACCCGGCGCCTGCCTTGTCGCCT`	`21 bp deleted → no frameshift`
134	`CCATCTCCGCGGTCGCCGTCGAC----------------CCGGCGCCTGCCTTGTCGCCT`	`16 bp deleted → frameshift`
134	`CCATCTCCGCGGTCGCC------------------------------------------T`	`42 bp deleted → no frameshift`
133	`CCATCTCCGCGGTCGCCGTCGACACGGC----------------------CTTGTCGCCT`	`22 bp deleted → frameshift`
133	`CCATCTCCGCGG----------------------GCTACCCGGCGCCTGCCTTGTCGCCT`	`22 bp deleted → frameshift`
127	`CCATCTCCGCGG-------------------------------CGCCTGCCTTGTCGCCT`	`31 bp deleted → frameshift`
126	`CCATCTCCGCGGTCGCC-----------------------CGGCGCCTGCCTTGTCGCCT`	`23 bp deleted → frameshift`
126	`CCATCTCCGCGG-----------------------CTACCCGGCGCCTGCCTTGTCGCCT`	`23 bp deleted → frameshift`
120	`CCATCTCCGCGGTCGCCGTCG------------------------CCTGCCTTGTCGCCT`	`24 bp deleted → no frameshift`
115	`CCATCTCCGCGGTCGCCGTCG------------------------------------CCT`	`36 bp deleted → no frameshift`
115	`CCATCTCCG---------------------------------GCGCCTGCCTTGTCGCCT`	`33 bp deleted → no frameshift`
109	`CCATCTCCGCGGTCGCC----------------------------------TTGTCGCCT`	`34 bp deleted → frameshift`
109	`CCATCTCCGCG----------------------------------CCTGCCTTGTCGCCT`	`34 bp deleted → frameshift`
109	`CCATCTCCGC--------------------------TACCCGGCGCCTGCCTTGTCGCCT`	`26 bp deleted → frameshift`
103	`CCATCTCCGCGGTCGCCG---------------------------CCTGCCTTGTCGCCT`	`27 bp deleted → no frameshift`
103	`CCATCTCCGCGGTCG---------------------------GCGCCTGCCTTGTCGCCT`	`27 bp deleted → no frameshift`
99	`CCATCTCCGC------------------------------------CTGCCTTGTCGCCT`	`36 bp deleted → no frameshift`
98	`CCATCTCCGCGGTCGC----------------------------GCCTGCCTTGTCGCCT`	`28 bp deleted → frameshift`
89	`CCATCTCCGCGGTCGCCGTCGACACGGC------------------------------CT`	`30 bp deleted → no frameshift`
84	`CCATCTCCGCGGTCGCCGTCGACACG-------------------------------CCT`	`31 bp deleted → frameshift`
80	`CCATCTCC--------------------------------CGGCGCCTGCCTTGTCGCCT`	`32 bp deleted → frameshift`
63	`CCATCT-------------------------------ACCCGGCGCCTGCCTTGTCGCCT`	`31 bp deleted → frameshift`
60	`CC--------------------------------------CGGCGCCTGCCTTGTCGCCT`	`38 bp deleted → frameshift`
56	`CCATCTCCGCGGTCGCCG---------------------------------------CCT`	`39 bp deleted → no frameshift`
56	`CCATCTCC---------------------------------------TGCCTTGTCGCCT`	`39 bp deleted → no frameshift`
56	`CC---------------------------------------GGCGCCTGCCTTGTCGCCT`	`39 bp deleted → no frameshift`
54	`CCATCTCCGC------------------------------------------------CT`	`48 bp deleted → no frameshift`
54	`CCATCTCCGC----------------------------------------CTTGTCGCCT`	`40 bp deleted → frameshift`
46	`CCATCTCC-------------------------------------------TTGTCGCCT`	`43 bp deleted → frameshift`
42	`CC---------------------------------------------TGCCTTGTCGCCT`	`45 bp deleted → no frameshift`
40	`CCATCTCCGCG----------------------------------------------CCT`	`46 bp deleted → frameshift`
36	`CCATCT------------------------------------------GCCTTGTCGCCT`	`42 bp deleted → no frameshift`
34	`CC-------------------------------------------------TTGTCGCCT`	`49 bp deleted → frameshift`
31	`CCATCTCC---------------------------------------------------T`	`51 bp deleted → no frameshift`
30	`CCATCT----------------------------------------------TGTCGCCT`	`46 bp deleted → frameshift`
25	`CCATCTC-------------------------------------------------GCCT`	`49 bp deleted → frameshift`
23	`CCATC---------------------------------------------------GCCT`	`51 bp deleted → no frameshift`
23	`CC---------------------------------------------------------T`	`57 bp deleted → no frameshift`
20	`CCATCT------------------------------------------------------`	`54 bp deleted → no frameshift`

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