CRISPOR

DNA break repair outcome predictions

Guide: AAAGGACATGGTTATGTTTC TGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

mh- Score	Sequence	Effect
	`AGTCCGGGGATGGAGAGCCGAAAGGACATGGTTATGTTTCTGGATGGGGGTCAGCTTGGCACTCTGGTTGGTAAGAGGGT`	(Wild-type sequence)
274	`TGGAGAGCCGAAAGGACATGG------------ATGGGGGTCAGCTTGGCACTCTGGTTG`	`12 bp deleted → no frameshift`
269	`TGGAGAGCCGAAAGGACATGG----------------GGGTCAGCTTGGCACTCTGGTTG`	`16 bp deleted → frameshift`
242	`TGGAGAGCCGAAAGGACATGGTTATG----------GGGGTCAGCTTGGCACTCTGGTTG`	`10 bp deleted → frameshift`
222	`TGGAGAGCCGAAAGGACATGGTTATG------GATGGGGGTCAGCTTGGCACTCTGGTTG`	`6 bp deleted → no frameshift`
203	`TGGAGAGCCGAAAGGA------------------TGGGGGTCAGCTTGGCACTCTGGTTG`	`18 bp deleted → no frameshift`
193	`TGGAGAGCCGAAAGGACATGGT-------------------CAGCTTGGCACTCTGGTTG`	`19 bp deleted → frameshift`
170	`TGGAGAGCCGAAAGGACATGG-----------------GGTCAGCTTGGCACTCTGGTTG`	`17 bp deleted → frameshift`
164	`TGGAGAGCCGAAAGGACATGGTTATGTTTC------------AGCTTGGCACTCTGGTTG`	`12 bp deleted → no frameshift`
162	`TGGAGAGCCGAAAGGACATGG------------------GTCAGCTTGGCACTCTGGTTG`	`18 bp deleted → no frameshift`
149	`TGGAGAGCCGAAAGGACATGGTTATGT--------------CAGCTTGGCACTCTGGTTG`	`14 bp deleted → frameshift`
133	`TGGA------------------------------TGGGGGTCAGCTTGGCACTCTGGTTG`	`30 bp deleted → no frameshift`
133	`TGGAGAGCCGAAAGG----------------------GGGTCAGCTTGGCACTCTGGTTG`	`22 bp deleted → frameshift`
126	`TGGAGAGCCGAAAGG-----------------------GGTCAGCTTGGCACTCTGGTTG`	`23 bp deleted → frameshift`
123	`TGGAGAGCCGAAAGGACATGG----------------------------CACTCTGGTTG`	`28 bp deleted → frameshift`
120	`TGGAGAGCCGAAAGG------------------------GTCAGCTTGGCACTCTGGTTG`	`24 bp deleted → no frameshift`
115	`TGGAGAGCCGAAAGGACATGGTT------------------------------------G`	`36 bp deleted → no frameshift`
114	`TGGAGAGCCGAAAGG-------------------------TCAGCTTGGCACTCTGGTTG`	`25 bp deleted → frameshift`
99	`TGGAGAGCCGAAAGGACATGGTTATG----------------------GCACTCTGGTTG`	`22 bp deleted → frameshift`
95	`TGGAGAGCCGA-----------------------TGGGGGTCAGCTTGGCACTCTGGTTG`	`23 bp deleted → frameshift`
91	`TGG----------------------------------GGGTCAGCTTGGCACTCTGGTTG`	`34 bp deleted → frameshift`
90	`TGGAGAGCCGAAAGGACATGGTTATGTTTC------------------------TGGTTG`	`24 bp deleted → no frameshift`
86	`TGGAGAGCCGAAAGGACA-------------------------GCTTGGCACTCTGGTTG`	`25 bp deleted → frameshift`
84	`TGGAGAGCCGAAAGGACATGGTTATGTT-------------------------------G`	`31 bp deleted → frameshift`
81	`TGGAGAGCCGAAAGGACATGGTTATGTTT------------------GGCACTCTGGTTG`	`18 bp deleted → no frameshift`
78	`TGGAGAGC-------------------------------------TTGGCACTCTGGTTG`	`37 bp deleted → frameshift`
77	`TGGAGAGCCGAAAGGACATGGTTATGTT-------------------GGCACTCTGGTTG`	`19 bp deleted → frameshift`
74	`TGGAGA----------------------------TGGGGGTCAGCTTGGCACTCTGGTTG`	`28 bp deleted → frameshift`
73	`TGGAGAGCCGAAAGG----------------------------------CACTCTGGTTG`	`34 bp deleted → frameshift`
69	`TGG-----------------------------------GGTCAGCTTGGCACTCTGGTTG`	`35 bp deleted → frameshift`
66	`TGGAGAGCCGAAAGGACATGGTTATG------------------------------GTTG`	`30 bp deleted → no frameshift`
66	`TGGAGAGCCGAAAG------------------------------CTTGGCACTCTGGTTG`	`30 bp deleted → no frameshift`
66	`TGG------------------------------------GTCAGCTTGGCACTCTGGTTG`	`36 bp deleted → no frameshift`
62	`TGG-------------------------------------TCAGCTTGGCACTCTGGTTG`	`37 bp deleted → frameshift`
60	`TGGAGAGCCGAAAGGACATGGTT------------------------GGCACTCTGGTTG`	`24 bp deleted → no frameshift`
57	`TGGAGAGCCGAAAGGACA---------------------------------CTCTGGTTG`	`33 bp deleted → no frameshift`
54	`TGGAGAGCCGAAAGGACATGGTTATG----------------------------------`	`34 bp deleted → frameshift`
52	`TGGAGAGCCGAAAGGAC-----------------------------------TCTGGTTG`	`35 bp deleted → frameshift`
50	`TGG----------------------------------------------CACTCTGGTTG`	`46 bp deleted → frameshift`
48	`TGGAGAGCCGAAAGG------------------------------------------TTG`	`42 bp deleted → no frameshift`
48	`TGGAGAGC------------------------------------------ACTCTGGTTG`	`42 bp deleted → no frameshift`
44	`TGGAGAGCCGAAAGGACATGGTTATGTTT------------------------------G`	`30 bp deleted → no frameshift`
42	`TGGAG---------------------------------------CTTGGCACTCTGGTTG`	`39 bp deleted → no frameshift`
40	`TGGAGAGCCGAAAGGACATG----------------------------------------`	`40 bp deleted → frameshift`
33	`TGG------------------------------------------------------TTG`	`54 bp deleted → no frameshift`
16	`TG----------------------------------------------------------`	`58 bp deleted → frameshift`

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