CRISPOR

DNA break repair outcome predictions

Guide: TGCACCCCAGACTGCGCCAC CGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

mh- Score	Sequence	Effect
	`ATGAAGAAATCGAGGTGAGCTGCACCCCAGACTGCGCCACCGGGAACGCCGAGTACCAGCACAGCAAAGGTAGCCACCTT`	(Wild-type sequence)
439	`CGAGGTGAGCTGCACCCCAGACTGCGCC------------GAGTACCAGCACAGCAAAGG`	`12 bp deleted → no frameshift`
296	`CGAGGTGAGCTGCACCCCAGACTGCG------GGAACGCCGAGTACCAGCACAGCAAAGG`	`6 bp deleted → no frameshift`
211	`CGAGGTGAGCTGCACCCCAGACTGCGCCAC-------GCCGAGTACCAGCACAGCAAAGG`	`7 bp deleted → frameshift`
198	`CGAGGTGAGCTGCACCCCAGACTGC--------------CGAGTACCAGCACAGCAAAGG`	`14 bp deleted → frameshift`
193	`CGAGGTGAGCTGCACCCCAGACTGCGCCA-------------------GCACAGCAAAGG`	`19 bp deleted → frameshift`
188	`CGAGGTGAGCTGCACCCCAGACTGCG---------------AGTACCAGCACAGCAAAGG`	`15 bp deleted → no frameshift`
166	`CGAGGTGAGCTGCACCCCAGACTGCGCCAC----------------------AGCAAAGG`	`22 bp deleted → frameshift`
164	`CGAGGTGAGCTGCACCCCAG-----------------------------CACAGCAAAGG`	`29 bp deleted → frameshift`
149	`CGAGGTGAGCTGCACCCCAGA--------------ACGCCGAGTACCAGCACAGCAAAGG`	`14 bp deleted → frameshift`
141	`CGAGGTGAGCTGCACCCCAGAC---------------GCCGAGTACCAGCACAGCAAAGG`	`15 bp deleted → no frameshift`
134	`CGAGGTGAGCTGCACCCCAGACTGCGCCAC----------------CAGCACAGCAAAGG`	`16 bp deleted → frameshift`
133	`CGAGGTGAGCTGCACCCC----------------------GAGTACCAGCACAGCAAAGG`	`22 bp deleted → frameshift`
126	`CGAGGTGAGCTGCACCCCAGACTGCGC-----------------------ACAGCAAAGG`	`23 bp deleted → frameshift`
126	`CGAGGTGAGCTGCACCC-----------------------GAGTACCAGCACAGCAAAGG`	`23 bp deleted → frameshift`
120	`CGAGGTGAGCTGCACC------------------------GAGTACCAGCACAGCAAAGG`	`24 bp deleted → no frameshift`
114	`CGAGGTGAGCTGCACCCCAGACTGC-------------------------ACAGCAAAGG`	`25 bp deleted → frameshift`
109	`CGAGGTGAGCTGCAC-------------------------------------AGCAAAGG`	`37 bp deleted → frameshift`
109	`CGAGGTGAGCTGC--------------------------CGAGTACCAGCACAGCAAAGG`	`26 bp deleted → frameshift`
106	`CGAGGTGAGCTGCACC-------------------------------AGCACAGCAAAGG`	`31 bp deleted → frameshift`
105	`CGAGGTGAGCTGCACCCCAGA---------------------GTACCAGCACAGCAAAGG`	`21 bp deleted → no frameshift`
99	`CGAGGTGAGCTGCAC----------------------GCCGAGTACCAGCACAGCAAAGG`	`22 bp deleted → frameshift`
99	`CGAG---------------------------------------TACCAGCACAGCAAAGG`	`39 bp deleted → no frameshift`
98	`CGAGGTGAGCTGCACCCCAGACTGCGC----------------------------AAAGG`	`28 bp deleted → frameshift`
98	`CGAGG----------------------------GAACGCCGAGTACCAGCACAGCAAAGG`	`28 bp deleted → frameshift`
95	`CGAGGTGAGCTGCACCCCAG-----------------------TACCAGCACAGCAAAGG`	`23 bp deleted → frameshift`
94	`CGAGGTGAGC-----------------------------CGAGTACCAGCACAGCAAAGG`	`29 bp deleted → frameshift`
94	`CGAGG-----------------------------AACGCCGAGTACCAGCACAGCAAAGG`	`29 bp deleted → frameshift`
91	`CGAGGTGAGCTGCACCCCAG----------------------------------CAAAGG`	`34 bp deleted → frameshift`
91	`CGAGGTGAG----------------------------------TACCAGCACAGCAAAGG`	`34 bp deleted → frameshift`
90	`CGAGGTGAGCTGCACCCCAGACTGCGCCA------------------------GCAAAGG`	`24 bp deleted → no frameshift`
90	`CGAGGTGAGCTGCACCCCAGAC------------------------CAGCACAGCAAAGG`	`24 bp deleted → no frameshift`
89	`CGAGGTGAGCTGCACCCCAGACTGC------------------------------AAAGG`	`30 bp deleted → no frameshift`
89	`CGAGGTGAGCTGCACCC------------------------------AGCACAGCAAAGG`	`30 bp deleted → no frameshift`
89	`CG------------------------------GGAACGCCGAGTACCAGCACAGCAAAGG`	`30 bp deleted → no frameshift`
77	`CGAGGTGAGCTGCACCCCAGACTGCGCCA---------------------------AAGG`	`27 bp deleted → no frameshift`
77	`CGAGGTGA---------------------------ACGCCGAGTACCAGCACAGCAAAGG`	`27 bp deleted → no frameshift`
67	`CGAGGTGAGC----------------------------------------ACAGCAAAGG`	`40 bp deleted → frameshift`
66	`CGAGGTGAGCTGCACCCCAGAC------------------------------AGCAAAGG`	`30 bp deleted → no frameshift`
66	`CG------------------------------------CCGAGTACCAGCACAGCAAAGG`	`36 bp deleted → no frameshift`
61	`CGAGGTGAGCTGCA------------------------------------------AAGG`	`42 bp deleted → no frameshift`
60	`CGAGGTGAGCTGCACCCCA--------------------------------CAGCAAAGG`	`32 bp deleted → frameshift`
60	`CGA--------------------------------ACGCCGAGTACCAGCACAGCAAAGG`	`32 bp deleted → frameshift`
54	`CGAGGTGAGCTGCA----------------------------------GCACAGCAAAGG`	`34 bp deleted → frameshift`
52	`CGAGGTGAGC---------------------------------------------AAAGG`	`45 bp deleted → no frameshift`
47	`CGAGGTGAGCTGCACCCCA-------------------------------------AAGG`	`37 bp deleted → frameshift`
45	`CGAGGT--------------------------------------ACCAGCACAGCAAAGG`	`38 bp deleted → frameshift`
42	`CGAGGTGAGCTGCACCCCAG---------------------------------------G`	`39 bp deleted → no frameshift`
42	`CGAGGTGAGCTGCA---------------------------------------GCAAAGG`	`39 bp deleted → no frameshift`
32	`CGAGG-------------------------------------------------------`	`55 bp deleted → frameshift`
31	`CGAG---------------------------------------------CACAGCAAAGG`	`45 bp deleted → no frameshift`
24	`CGAGGTGAG--------------------------------------------------G`	`50 bp deleted → frameshift`
24	`CGAG--------------------------------------------------CAAAGG`	`50 bp deleted → frameshift`

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