CRISPOR

DNA break repair outcome predictions

Guide: ACTTCCCGGGAGCGTGGCGG GGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

mh- Score	Sequence	Effect
	`GCCCGCGAGCCGGCCCTCCGACTTCCCGGGAGCGTGGCGGGGGAGGCCAGGAGGAGGAGCCGGTCGATGGACTAGCAGGC`	(Wild-type sequence)
469	`CGGCCCTCCGACTTCCCGGGAG-------------GCCAGGAGGAGGAGCCGGTCGATGG`	`13 bp deleted → frameshift`
382	`CGGCCCTCCGACTTCCCGGGAGCGTGGC---------CAGGAGGAGGAGCCGGTCGATGG`	`9 bp deleted → no frameshift`
362	`CGGCCCTCCGACTTCCCGGGAGCGTGGCGG--GAGGCCAGGAGGAGGAGCCGGTCGATGG`	`2 bp deleted → frameshift`
344	`CGGCCCTCCGACTTCCCGGGAGCGTGGCGG---AGGCCAGGAGGAGGAGCCGGTCGATGG`	`3 bp deleted → no frameshift`
311	`CGGCCCTCCGACTTCCCGGGAGCGTGG-----GAGGCCAGGAGGAGGAGCCGGTCGATGG`	`5 bp deleted → frameshift`
296	`CGGCCCTCCGACTTCCCGGGAGCGTGGCGG------CCAGGAGGAGGAGCCGGTCGATGG`	`6 bp deleted → no frameshift`
296	`CGGCCCTCCGACTTCCCGGGAGCGTGG------AGGCCAGGAGGAGGAGCCGGTCGATGG`	`6 bp deleted → no frameshift`
245	`CGGCCCTCCGACTTCCCGGGAG---------------------GAGGAGCCGGTCGATGG`	`21 bp deleted → no frameshift`
233	`CGGCCCTCCGACTTCCCGGGAGC---------------------------CGGTCGATGG`	`27 bp deleted → no frameshift`
230	`CGGCCCTCCGACTTCCCGGGAGCGTGGCGG-----------AGGAGGAGCCGGTCGATGG`	`11 bp deleted → frameshift`
219	`CGGCCCTCCGACTTCCCGGG------------GAGGCCAGGAGGAGGAGCCGGTCGATGG`	`12 bp deleted → no frameshift`
210	`CGGCCCTCCGACTTCCCGGGAG------------------------GAGCCGGTCGATGG`	`24 bp deleted → no frameshift`
198	`CGGCCCTCCGACTTCCCGGGAGCGTGGCGG--------------AGGAGCCGGTCGATGG`	`14 bp deleted → frameshift`
198	`CGGCCCTCCGACTTCCCGGGAGCGTGG--------------AGGAGGAGCCGGTCGATGG`	`14 bp deleted → frameshift`
198	`CGGCCCTCCGACTTCCCGGGAGC--------------CAGGAGGAGGAGCCGGTCGATGG`	`14 bp deleted → frameshift`
198	`CGGCCCTCCGACTTCCCGG--------------AGGCCAGGAGGAGGAGCCGGTCGATGG`	`14 bp deleted → frameshift`
190	`CGGCCCTCCGACTTCCCGGGAGCGTGGCGG-----------------------TCGATGG`	`23 bp deleted → frameshift`
179	`CGGCCCTCCGACTTCCCGGG----------------CCAGGAGGAGGAGCCGGTCGATGG`	`16 bp deleted → frameshift`
170	`CGGCCCTCCGACTTCCCGGGAGCGTGGCGG-----------------AGCCGGTCGATGG`	`17 bp deleted → frameshift`
170	`CGGCCCTCCGACTTCCCGGGAGCGTGG-----------------AGGAGCCGGTCGATGG`	`17 bp deleted → frameshift`
170	`CGGCCCTCCGACTTCCCGG-----------------CCAGGAGGAGGAGCCGGTCGATGG`	`17 bp deleted → frameshift`
153	`CGGCC---------------------------------AGGAGGAGGAGCCGGTCGATGG`	`33 bp deleted → no frameshift`
147	`CGGCCCTCCGACTTCCCGGGAGCGTGG--------------------AGCCGGTCGATGG`	`20 bp deleted → frameshift`
146	`CGGCCCTCCGACTTCCCGG----------------------------------TCGATGG`	`34 bp deleted → frameshift`
140	`CGGCCCTCCGACTTCCC---------------------AGGAGGAGGAGCCGGTCGATGG`	`21 bp deleted → no frameshift`
133	`CGGCCCTCCGACTTCCCGGGAGCGTGGC----------------------CGGTCGATGG`	`22 bp deleted → frameshift`
133	`CGGCCCTCCGACTTCCCGG----------------------AGGAGGAGCCGGTCGATGG`	`22 bp deleted → frameshift`
133	`CGGCCCTCCGACTTCC----------------------AGGAGGAGGAGCCGGTCGATGG`	`22 bp deleted → frameshift`
122	`CGGCCCTCCGACTTCCCGGGAG------------------GAGGAGGAGCCGGTCGATGG`	`18 bp deleted → no frameshift`
114	`CGGCCCTCCGACTTCCCGG-------------------------AGGAGCCGGTCGATGG`	`25 bp deleted → frameshift`
109	`CGGCCCTCCGACTTCCCGGGAGCGTGG--------------------------TCGATGG`	`26 bp deleted → frameshift`
103	`CGGCCCTCCGACTTCCCGGGAGCGTGGCG---------------------------ATGG`	`27 bp deleted → no frameshift`
98	`CGGCCCTCCGACTTCCCGGGAGCG----------------------------GTCGATGG`	`28 bp deleted → frameshift`
98	`CGGCCCTCCGACTTCCCGG----------------------------AGCCGGTCGATGG`	`28 bp deleted → frameshift`
96	`CGGCCCTCCGACTTCCCGGGAGCGTGG---------------------------------`	`33 bp deleted → no frameshift`
95	`CGGCCCTCCGA-----------------------GGCCAGGAGGAGGAGCCGGTCGATGG`	`23 bp deleted → frameshift`
94	`CGGCCCTCC-----------------------------AGGAGGAGGAGCCGGTCGATGG`	`29 bp deleted → frameshift`
94	`CGG-----------------------------GAGGCCAGGAGGAGGAGCCGGTCGATGG`	`29 bp deleted → frameshift`
89	`CGGCCCTCCGACTTCCCGGGAGCGTGGCGG------------------------------`	`30 bp deleted → no frameshift`
89	`CGG------------------------------AGGCCAGGAGGAGGAGCCGGTCGATGG`	`30 bp deleted → no frameshift`
80	`CGGCCCTCCGACTTCCCGGGAGCG--------------------------------ATGG`	`32 bp deleted → frameshift`
80	`CGGCCC--------------------------------AGGAGGAGGAGCCGGTCGATGG`	`32 bp deleted → frameshift`
76	`CGGCCCTCCGACTTCCCGGG---------------------------------TCGATGG`	`33 bp deleted → no frameshift`
73	`CGGCCCTCCG------------------------------------------GTCGATGG`	`42 bp deleted → no frameshift`
70	`CGGCCCTCCGACTTCCCGGGAGCGT-----------------------------CGATGG`	`29 bp deleted → frameshift`
69	`CGGCCCTCCGACTTCC-----------------------------------GGTCGATGG`	`35 bp deleted → frameshift`
63	`CGGCCCTCCGA-------------------------------GGAGGAGCCGGTCGATGG`	`31 bp deleted → frameshift`
60	`CGGCCCTCCGACTTCCCG--------------------------------------ATGG`	`38 bp deleted → frameshift`
60	`CGGCC----------------------------------------------GGTCGATGG`	`46 bp deleted → frameshift`
60	`CGG--------------------------------------AGGAGGAGCCGGTCGATGG`	`38 bp deleted → frameshift`
54	`CGGCCCTCCGA----------------------------------GGAGCCGGTCGATGG`	`34 bp deleted → frameshift`
54	`CGGCCCTCCGACTTCCCGGG----------------------------------------`	`40 bp deleted → frameshift`
51	`CGGCCCTCCGACTTCCCGG-----------------------------------------`	`41 bp deleted → frameshift`
51	`CGG-----------------------------------------AGGAGCCGGTCGATGG`	`41 bp deleted → frameshift`
50	`CGGCCCTCCGA----------------------------------------------TGG`	`46 bp deleted → frameshift`
49	`CGGCCCTCCGACTTCCCGGGA------------------------------------TGG`	`36 bp deleted → no frameshift`
49	`CGG--------------------------------------------------TCGATGG`	`50 bp deleted → frameshift`
47	`CGGCCCTCCGA-------------------------------------GCCGGTCGATGG`	`37 bp deleted → frameshift`
44	`CGG--------------------------------------------AGCCGGTCGATGG`	`44 bp deleted → frameshift`
42	`CGGCCC---------------------------------------------GGTCGATGG`	`45 bp deleted → no frameshift`
40	`CGGCCCTCCGACTTC----------------------------------------GATGG`	`40 bp deleted → frameshift`
28	`CGGCCCTC-----------------------------------------------GATGG`	`47 bp deleted → frameshift`
26	`CG------------------------------------------------------ATGG`	`54 bp deleted → no frameshift`
23	`CGG---------------------------------------------------------`	`57 bp deleted → no frameshift`

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