CRISPOR

DNA break repair outcome predictions

Guide: ACCATCTTCTCTGGCGGTTC AGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

mh- Score	Sequence	Effect
	`GGCTCAGGCAACCGTGGGGCACCATCTTCTCTGGCGGTTCAGGCAGCGGGCTGCTCACGGCTTCGGACAAATTAGAGACC`	(Wild-type sequence)
439	`ACCGTGGGGCACCATCTTCTCTGGCGG------------GCTGCTCACGGCTTCGGACAA`	`12 bp deleted → no frameshift`
382	`ACCGTGGGGCACCATCTTCTCTGGC---------AGCGGGCTGCTCACGGCTTCGGACAA`	`9 bp deleted → no frameshift`
296	`ACCGTGGGGCACCATCTTCTCTGGCGG------CAGCGGGCTGCTCACGGCTTCGGACAA`	`6 bp deleted → no frameshift`
269	`ACCGTGGGGCACCATCTTCTCTGGC----------------TGCTCACGGCTTCGGACAA`	`16 bp deleted → frameshift`
210	`ACCGTGGGGCA------------------------GCGGGCTGCTCACGGCTTCGGACAA`	`24 bp deleted → no frameshift`
208	`ACCGTGGGGCACCATCTTCTCTGGCGG-------------CTGCTCACGGCTTCGGACAA`	`13 bp deleted → frameshift`
190	`ACCGTGGGGCACCATCTTCTCTGGCGG-----------------------CTTCGGACAA`	`23 bp deleted → frameshift`
188	`ACCGTGGGGCACCATCTTCTCTGG---------------GCTGCTCACGGCTTCGGACAA`	`15 bp deleted → no frameshift`
184	`ACCGTGGGGCACCATCTTCTCTG--------------------CTCACGGCTTCGGACAA`	`20 bp deleted → frameshift`
169	`ACCGTGGGGC-------------------------------TGCTCACGGCTTCGGACAA`	`31 bp deleted → frameshift`
163	`ACCGTGGGGCACCATCTTCTCTGGC--------------------------TTCGGACAA`	`26 bp deleted → frameshift`
154	`ACCGTGGGGCACCATCTTCTCTGGC-------------------TCACGGCTTCGGACAA`	`19 bp deleted → frameshift`
143	`ACCGTGGGGCACCATCTTCTC-------------------------ACGGCTTCGGACAA`	`25 bp deleted → frameshift`
141	`ACCGTGGGGCACCATCTTCTCTGGCGG-----------------------------ACAA`	`29 bp deleted → frameshift`
134	`ACCGTGGGGCACCATCTTCTCTGGCGGTTC----------------ACGGCTTCGGACAA`	`16 bp deleted → frameshift`
121	`ACCGTGGG--------------------------------CTGCTCACGGCTTCGGACAA`	`32 bp deleted → frameshift`
120	`ACCGTGGGGCACCATCTTCTCTGGCGGTTC------------------------GGACAA`	`24 bp deleted → no frameshift`
114	`ACCGTGGG-------------------------CAGCGGGCTGCTCACGGCTTCGGACAA`	`25 bp deleted → frameshift`
109	`ACCGTGG--------------------------CAGCGGGCTGCTCACGGCTTCGGACAA`	`26 bp deleted → frameshift`
105	`ACCGTGGGGCACCA---------------------GCGGGCTGCTCACGGCTTCGGACAA`	`21 bp deleted → no frameshift`
104	`ACCGTGGGGCACCATCTTC-----------------------------------GGACAA`	`35 bp deleted → frameshift`
103	`ACCGTGGGGC---------------------------GGGCTGCTCACGGCTTCGGACAA`	`27 bp deleted → no frameshift`
99	`ACCGTGGGGCACCATCTTCT----------------------GCTCACGGCTTCGGACAA`	`22 bp deleted → frameshift`
95	`ACCGTGGGGCACCATCTTCTCT-----------------------CACGGCTTCGGACAA`	`23 bp deleted → frameshift`
89	`ACCGTGGGG------------------------------GCTGCTCACGGCTTCGGACAA`	`30 bp deleted → no frameshift`
86	`ACCGTGGGGCACCATCT-------------------------GCTCACGGCTTCGGACAA`	`25 bp deleted → frameshift`
82	`ACCGTGGGGCAC------------------------------------GGCTTCGGACAA`	`36 bp deleted → no frameshift`
80	`ACCGTGGGGCACCATCTTCTCTGG--------------------------------ACAA`	`32 bp deleted → frameshift`
77	`ACCGTGGGGCACCATCTTC---------------------------ACGGCTTCGGACAA`	`27 bp deleted → no frameshift`
77	`ACCGTGGGGC-----------------------------------------TTCGGACAA`	`41 bp deleted → frameshift`
76	`ACCGTGG---------------------------------CTGCTCACGGCTTCGGACAA`	`33 bp deleted → no frameshift`
74	`ACCGTGGGGCACCATCT----------------------------CACGGCTTCGGACAA`	`28 bp deleted → frameshift`
73	`ACCGTGGGGC----------------------------------TCACGGCTTCGGACAA`	`34 bp deleted → frameshift`
73	`ACCG----------------------------------GGCTGCTCACGGCTTCGGACAA`	`34 bp deleted → frameshift`
66	`ACCGTGGGGCACCATCTTCTCT------------------------------TCGGACAA`	`30 bp deleted → no frameshift`
66	`ACCGTGGGGCACCATC------------------------------ACGGCTTCGGACAA`	`30 bp deleted → no frameshift`
60	`ACCGTGGGGCACCATCTTCT--------------------------------TCGGACAA`	`32 bp deleted → frameshift`
57	`ACCGTGGGGCACCATCTTCTC---------------------------------GGACAA`	`33 bp deleted → no frameshift`
57	`ACCGTGGGGCACCA---------------------------------CGGCTTCGGACAA`	`33 bp deleted → no frameshift`
48	`ACCGTGGG------------------------------------------CTTCGGACAA`	`42 bp deleted → no frameshift`
47	`ACCGTG-------------------------------------CTCACGGCTTCGGACAA`	`37 bp deleted → frameshift`
46	`ACCGTGG-------------------------------------------CTTCGGACAA`	`43 bp deleted → frameshift`
45	`ACCGTGGGGCACCATC--------------------------------------GGACAA`	`38 bp deleted → frameshift`
42	`ACCG---------------------------------------------GCTTCGGACAA`	`45 bp deleted → no frameshift`
38	`ACCGTGGGG-----------------------------------------------ACAA`	`47 bp deleted → frameshift`
36	`ACCGTGGG------------------------------------------------ACAA`	`48 bp deleted → no frameshift`
34	`ACCGTGG-------------------------------------------------ACAA`	`49 bp deleted → frameshift`
31	`ACCGTGGGGCACCA---------------------------------------------A`	`45 bp deleted → no frameshift`
31	`ACCG---------------------------------------------------GACAA`	`51 bp deleted → no frameshift`
30	`ACCGTGGGGCAC----------------------------------------------AA`	`46 bp deleted → frameshift`
30	`AC----------------------------------------------GGCTTCGGACAA`	`46 bp deleted → frameshift`
27	`ACCGTGGGGCA------------------------------------------------A`	`48 bp deleted → no frameshift`
18	`AC--------------------------------------------------------AA`	`56 bp deleted → frameshift`

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