← return to the list of all guides
Contents:In the table below, Illumina Nextera Handle sequences have been added and highlighted in bold. Primers for the on-target have been added for convenience. The table below is sorted by the CFD off-target score. Sites with very low CFD scores < 0.02 are unlikely to be cleaved, see our study Haeussler et al. 2016, Figure 2.
In the protocol by Matthew Canver, Harvard, two PCRs are run: one PCR to amplify the potential off-target, then a second PCR to extend the handles with Illumina barcodes. Please click here to download the protocol. Alternatively, you can have a look at Fu et al, 2014.
If a primer was not found, the reason is usually that the region around the off-target is too repetitive. To avoid unspecific primers, all repeats are masked for the primer design (not for off-target search). If you think that we should change the parameters here or should use different primer3 settings, please let us know.
| Name | Primer Sequence | Tm | CFD Score |
|---|---|---|---|
| ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52314145_F | TCGTCGGCAGCGTCGTCGATGGACTAGCAGGCAG | 60.2 | 1.00 |
| ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52314145_R | GTCTCGTGGGCTCGGTCGGTGTCTGAGCTACTAGGT | 59.7 | 1.00 |
| mm3_intron_Ccdc147_chr19_48001208_F | TCGTCGGCAGCGTCTCCTGCTGCAATGTCTCTGA | 59.0 | 0.70 |
| mm3_intron_Ccdc147_chr19_48001208_R | GTCTCGTGGGCTCGGGCAGACACTACCCCAAGGAG | 59.7 | 0.70 |
| mm3_intron_Dagla_chr19_10280443_F | Primer3: not found at this Tm | N.d. | 0.49 |
| mm3_intron_Dagla_chr19_10280443_R | Primer3: not found at this Tm | N.d. | 0.49 |
| mm4_intergenic_Gm13888|AI314831_chr2_105050800_F | TCGTCGGCAGCGTCCCACAAGTCCAGCCCATGAT | 60.0 | 0.44 |
| mm4_intergenic_Gm13888|AI314831_chr2_105050800_R | GTCTCGTGGGCTCGGGGGGCTTCTGGCTTCTGAAA | 60.2 | 0.44 |
| mm4_intergenic_Gas7|Gm12301_chr11_67565988_F | Primer3: not found at this Tm | N.d. | 0.41 |
| mm4_intergenic_Gas7|Gm12301_chr11_67565988_R | Primer3: not found at this Tm | N.d. | 0.41 |
| mm4_intergenic_Setdb1|Gm5070_chr3_95392857_F | Primer3: not found at this Tm | N.d. | 0.41 |
| mm4_intergenic_Setdb1|Gm5070_chr3_95392857_R | Primer3: not found at this Tm | N.d. | 0.41 |
| mm4_intergenic_Gm17070|Ptgdr_chr14_44844278_F | Primer3: not found at this Tm | N.d. | 0.37 |
| mm4_intergenic_Gm17070|Ptgdr_chr14_44844278_R | Primer3: not found at this Tm | N.d. | 0.37 |
| mm4_intron_Plb1_chr5_32243424_F | TCGTCGGCAGCGTCAGCCCCAGAATCTTTACAGATGG | 60.1 | 0.34 |
| mm4_intron_Plb1_chr5_32243424_R | GTCTCGTGGGCTCGGAAAGTCCACCTGGCAAGAGG | 59.8 | 0.34 |
| mm3_intergenic_Gm6317|Gm25383_chr13_78455202_F | TCGTCGGCAGCGTCAAACTGGCAGGCCAACTCTG | 60.8 | 0.33 |
| mm3_intergenic_Gm6317|Gm25383_chr13_78455202_R | GTCTCGTGGGCTCGGGACTCTGCCCAGGAAAGACC | 60.0 | 0.33 |
| mm4_intron_Chchd3_chr6_32995639_F | TCGTCGGCAGCGTCCCACAGGCTAAGTCAATCCCC | 60.4 | 0.33 |
| mm4_intron_Chchd3_chr6_32995639_R | GTCTCGTGGGCTCGGACAGCAGCAAGGACTCACTC | 59.9 | 0.33 |
| mm4_intron_Ccser1_chr6_62081186_F | Primer3: not found at this Tm | N.d. | 0.31 |
| mm4_intron_Ccser1_chr6_62081186_R | Primer3: not found at this Tm | N.d. | 0.31 |
| mm4_intergenic_Gm5072|Gm8914_chrX_91476156_F | Primer3: not found at this Tm | N.d. | 0.30 |
| mm4_intergenic_Gm5072|Gm8914_chrX_91476156_R | Primer3: not found at this Tm | N.d. | 0.30 |
| mm4_intergenic_Gm5071|Gm14784_chrX_91936926_F | Primer3: not found at this Tm | N.d. | 0.30 |
| mm4_intergenic_Gm5071|Gm14784_chrX_91936926_R | Primer3: not found at this Tm | N.d. | 0.30 |
| mm4_intergenic_Gm14783|Gm5072_chrX_91389260_F | Primer3: not found at this Tm | N.d. | 0.30 |
| mm4_intergenic_Gm14783|Gm5072_chrX_91389260_R | Primer3: not found at this Tm | N.d. | 0.30 |
| mm4_intergenic_4930594M17Rik|Gm26012_chr18_81660904_F | TCGTCGGCAGCGTCGGACCAGGAAAGGAAGCAGG | 60.3 | 0.30 |
| mm4_intergenic_4930594M17Rik|Gm26012_chr18_81660904_R | GTCTCGTGGGCTCGGTTTGCAGCCTCTCCTTCCTG | 59.9 | 0.30 |
| mm4_intergenic_Fgf10|Nnt_chr13_119266293_F | Primer3: not found at this Tm | N.d. | 0.30 |
| mm4_intergenic_Fgf10|Nnt_chr13_119266293_R | Primer3: not found at this Tm | N.d. | 0.30 |
| mm4_intron_Fam47e_chr5_92568450_F | TCGTCGGCAGCGTCAGCATGAGGTAACGCCACC | 60.0 | 0.30 |
| mm4_intron_Fam47e_chr5_92568450_R | GTCTCGTGGGCTCGGGGATGCCTCCTTCAGGGTTC | 60.1 | 0.30 |
| mm4_intergenic_Gm24932|Syt9_chr7_107344265_F | TCGTCGGCAGCGTCCCTGCTACTAGCTCCCCTCT | 59.8 | 0.29 |
| mm4_intergenic_Gm24932|Syt9_chr7_107344265_R | GTCTCGTGGGCTCGGTAGGAGGAACGGGCATCATG | 59.2 | 0.29 |
| mm4_intron_Mylk_chr16_34920339_F | Primer3: not found at this Tm | N.d. | 0.27 |
| mm4_intron_Mylk_chr16_34920339_R | Primer3: not found at this Tm | N.d. | 0.27 |
| mm4_intron_Slc24a2_chr4_87077803_F | TCGTCGGCAGCGTCTGCATCAGGTTACGGTAGGT | 58.4 | 0.26 |
| mm4_intron_Slc24a2_chr4_87077803_R | GTCTCGTGGGCTCGGTCGGGGTGAGAGGTAGTCTT | 59.2 | 0.26 |
| mm4_intergenic_Gm25779|Gm13173_chr4_153022722_F | Primer3: not found at this Tm | N.d. | 0.25 |
| mm4_intergenic_Gm25779|Gm13173_chr4_153022722_R | Primer3: not found at this Tm | N.d. | 0.25 |
| mm4_intergenic_Ntf3|Kcna5_chr6_126421572_F | Primer3: not found at this Tm | N.d. | 0.25 |
| mm4_intergenic_Ntf3|Kcna5_chr6_126421572_R | Primer3: not found at this Tm | N.d. | 0.25 |
| mm2_intergenic_Lingo1|Odf3l1_chr9_56792590_F | TCGTCGGCAGCGTCCTTCCCCACAGCATCAGGAC | 60.3 | 0.24 |
| mm2_intergenic_Lingo1|Odf3l1_chr9_56792590_R | GTCTCGTGGGCTCGGGCAGGGGTCAGGGATGAAAT | 59.7 | 0.24 |
| mm4_intron_Mov10l1_chr15_89003754_F | Primer3: not found at this Tm | N.d. | 0.24 |
| mm4_intron_Mov10l1_chr15_89003754_R | Primer3: not found at this Tm | N.d. | 0.24 |
| mm4_intron_Ppargc1b_chr18_61366847_F | Primer3: not found at this Tm | N.d. | 0.24 |
| mm4_intron_Ppargc1b_chr18_61366847_R | Primer3: not found at this Tm | N.d. | 0.24 |
| mm4_intron_Ccdc91_chr6_147544430_F | Primer3: not found at this Tm | N.d. | 0.24 |
| mm4_intron_Ccdc91_chr6_147544430_R | Primer3: not found at this Tm | N.d. | 0.24 |
| mm4_intergenic_Hsdl2|E130308A19Rik_chr4_59622495_F | Primer3: not found at this Tm | N.d. | 0.24 |
| mm4_intergenic_Hsdl2|E130308A19Rik_chr4_59622495_R | Primer3: not found at this Tm | N.d. | 0.24 |
| mm4_intergenic_Adra2a|Gpam_chr19_54928476_F | TCGTCGGCAGCGTCACCTGGGGATCTGCTTTGTG | 59.9 | 0.23 |
| mm4_intergenic_Adra2a|Gpam_chr19_54928476_R | GTCTCGTGGGCTCGGCTTAGGGTCAAAGGGTGGGG | 59.6 | 0.23 |
| mm4_intergenic_Ftl2/Mir692-2a|Grik3_chr4_125571676_F | Primer3: not found at this Tm | N.d. | 0.23 |
| mm4_intergenic_Ftl2/Mir692-2a|Grik3_chr4_125571676_R | Primer3: not found at this Tm | N.d. | 0.23 |
| mm4_exon_Kif13b_chr14_64806224_F | TCGTCGGCAGCGTCGAAGTGCTACCATCTCGGGC | 60.5 | 0.22 |
| mm4_exon_Kif13b_chr14_64806224_R | GTCTCGTGGGCTCGGCCACAAGGGAGGTGCATCTT | 59.9 | 0.22 |
| mm4_intergenic_Gm11978|Ramp3/Gm11978_chr11_6652177_F | Primer3: not found at this Tm | N.d. | 0.21 |
| mm4_intergenic_Gm11978|Ramp3/Gm11978_chr11_6652177_R | Primer3: not found at this Tm | N.d. | 0.21 |
| mm4_intergenic_Ssxb1|Ssxb2_chrX_8448607_F | Primer3: not found at this Tm | N.d. | 0.21 |
| mm4_intergenic_Ssxb1|Ssxb2_chrX_8448607_R | Primer3: not found at this Tm | N.d. | 0.21 |
| mm4_intergenic_Ssxb10|Ssxb9_chrX_8342297_F | Primer3: not found at this Tm | N.d. | 0.21 |
| mm4_intergenic_Ssxb10|Ssxb9_chrX_8342297_R | Primer3: not found at this Tm | N.d. | 0.21 |
| mm4_intergenic_Gm9430|Ssxb8_chrX_8679476_F | Primer3: not found at this Tm | N.d. | 0.21 |
| mm4_intergenic_Gm9430|Ssxb8_chrX_8679476_R | Primer3: not found at this Tm | N.d. | 0.21 |
| mm4_intergenic_Gm9430|Ssxb8_chrX_8664714_F | Primer3: not found at this Tm | N.d. | 0.21 |
| mm4_intergenic_Gm9430|Ssxb8_chrX_8664714_R | Primer3: not found at this Tm | N.d. | 0.21 |
| mm4_intergenic_Gm6592|Gm5751_chrX_8859451_F | Primer3: not found at this Tm | N.d. | 0.21 |
| mm4_intergenic_Gm6592|Gm5751_chrX_8859451_R | Primer3: not found at this Tm | N.d. | 0.21 |
| mm4_intergenic_Gm14459|Ssxb6_chrX_8535654_F | Primer3: not found at this Tm | N.d. | 0.21 |
| mm4_intergenic_Gm14459|Ssxb6_chrX_8535654_R | Primer3: not found at this Tm | N.d. | 0.21 |
| mm3_intron_Usp24_chr4_106408329_F | TCGTCGGCAGCGTCGTGAACTGATGGCCCTGGAG | 60.3 | 0.21 |
| mm3_intron_Usp24_chr4_106408329_R | GTCTCGTGGGCTCGGACTCTGGGCAAGTCTGTTGT | 59.1 | 0.21 |
| mm4_intergenic_Slc28a3|Ntrk2_chr13_58661382_F | TCGTCGGCAGCGTCGATGCGAAACCCCAAAGCAG | 60.1 | 0.19 |
| mm4_intergenic_Slc28a3|Ntrk2_chr13_58661382_R | GTCTCGTGGGCTCGGACAGATCTGGGGAAGAGGCC | 60.9 | 0.19 |
| mm4_intron_Inf2_chr12_112597806_F | Primer3: not found at this Tm | N.d. | 0.19 |
| mm4_intron_Inf2_chr12_112597806_R | Primer3: not found at this Tm | N.d. | 0.19 |
| mm4_intergenic_Cbx2|Cbx8_chr11_119033926_F | TCGTCGGCAGCGTCACCTCAGGTCAGGACAGGAA | 59.8 | 0.19 |
| mm4_intergenic_Cbx2|Cbx8_chr11_119033926_R | GTCTCGTGGGCTCGGACATCAATGTAGGCCAGCCC | 60.1 | 0.19 |
| mm4_intron_Tmem189/Gm20431_chr2_167653105_F | TCGTCGGCAGCGTCAGGAGGTGACCAGCCAGTAT | 59.9 | 0.19 |
| mm4_intron_Tmem189/Gm20431_chr2_167653105_R | GTCTCGTGGGCTCGGATTGTTCTCTTCCCACCGGC | 60.3 | 0.19 |
| mm4_intron_Zfp280c_chrX_48594081_F | TCGTCGGCAGCGTCCAAAGCCAGAGAGGTCCTGG | 60.0 | 0.18 |
| mm4_intron_Zfp280c_chrX_48594081_R | GTCTCGTGGGCTCGGGGGGTGCTTGTGGCGATC | 61.1 | 0.18 |
| mm3_intergenic_Gm22084|Gm24502_chr5_75374816_F | Primer3: not found at this Tm | N.d. | 0.18 |
| mm3_intergenic_Gm22084|Gm24502_chr5_75374816_R | Primer3: not found at this Tm | N.d. | 0.18 |
| mm4_intergenic_Gm5555|Gpr125_chr5_49955646_F | Primer3: not found at this Tm | N.d. | 0.18 |
| mm4_intergenic_Gm5555|Gpr125_chr5_49955646_R | Primer3: not found at this Tm | N.d. | 0.18 |
| mm4_intergenic_Gm25377|Hs3st1_chr5_39574871_F | Primer3: not found at this Tm | N.d. | 0.18 |
| mm4_intergenic_Gm25377|Hs3st1_chr5_39574871_R | Primer3: not found at this Tm | N.d. | 0.18 |
| mm4_intergenic_Gm833|Gm25779_chr4_152859956_F | Primer3: not found at this Tm | N.d. | 0.17 |
| mm4_intergenic_Gm833|Gm25779_chr4_152859956_R | Primer3: not found at this Tm | N.d. | 0.17 |
| mm4_intergenic_Hs3st5|Hdac2_chr10_36956415_F | Primer3: not found at this Tm | N.d. | 0.17 |
| mm4_intergenic_Hs3st5|Hdac2_chr10_36956415_R | Primer3: not found at this Tm | N.d. | 0.17 |
| mm4_intron_Cdc73_chr1_143636286_F | Primer3: not found at this Tm | N.d. | 0.16 |
| mm4_intron_Cdc73_chr1_143636286_R | Primer3: not found at this Tm | N.d. | 0.16 |
| mm4_intergenic_Psap|Cdh23_chr10_60302676_F | TCGTCGGCAGCGTCTCAGCCAGAAGAGAGTGGGT | 60.1 | 0.14 |
| mm4_intergenic_Psap|Cdh23_chr10_60302676_R | GTCTCGTGGGCTCGGAATACCCTGACTTGGTGCCG | 60.0 | 0.14 |
| mm4_intergenic_Gm22484|Gm23555_chr18_56189717_F | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_intergenic_Gm22484|Gm23555_chr18_56189717_R | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_intron_Gm872_chr10_93069427_F | TCGTCGGCAGCGTCAGGACACTGAACAGCCAGG | 59.2 | 0.13 |
| mm4_intron_Gm872_chr10_93069427_R | GTCTCGTGGGCTCGGCACAAAGGAGCCCAGACACT | 59.8 | 0.13 |
| mm4_intergenic_9230111E07Rik|1200007C13Rik_chr2_167824642_F | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_intergenic_9230111E07Rik|1200007C13Rik_chr2_167824642_R | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_intron_Crtac1_chr19_42401159_F | TCGTCGGCAGCGTCCACTGTTCTCAGGTCCGTGG | 60.3 | 0.12 |
| mm4_intron_Crtac1_chr19_42401159_R | GTCTCGTGGGCTCGGTGTGGGAGCTATGTAGGCCT | 60.0 | 0.12 |
| mm4_intergenic_Trib1|Gm7083_chr15_59660168_F | TCGTCGGCAGCGTCAGGTCCTTTCTGCTGTTGTT | 57.2 | 0.11 |
| mm4_intergenic_Trib1|Gm7083_chr15_59660168_R | GTCTCGTGGGCTCGGATGGCCCGGGTCTAGTTTTG | 60.0 | 0.11 |
| mm3_intergenic_Sptlc1|Msx2_chr13_53430622_F | TCGTCGGCAGCGTCTCACACAGTTCCAGCTGTCC | 59.8 | 0.10 |
| mm3_intergenic_Sptlc1|Msx2_chr13_53430622_R | GTCTCGTGGGCTCGGTTCTGAGCTCAACTGCCTGG | 59.9 | 0.10 |
| mm4_intergenic_Gm20388/Galnt2|1810008B01Rik/Gm20388/Galnt2_chr8_124301650_F | TCGTCGGCAGCGTCTGTTCCTGTGTGTAGCAGGC | 60.2 | 0.09 |
| mm4_intergenic_Gm20388/Galnt2|1810008B01Rik/Gm20388/Galnt2_chr8_124301650_R | GTCTCGTGGGCTCGGGGGCTCATGAGTCACACCTC | 60.1 | 0.09 |
| mm4_intergenic_4930474H06Rik|Gm22959_chr12_71653943_F | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intergenic_4930474H06Rik|Gm22959_chr12_71653943_R | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intergenic_Gm10480|5730507C01Rik_chr12_18382648_F | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intergenic_Gm10480|5730507C01Rik_chr12_18382648_R | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_exon_Gm13802_chr2_93098149_F | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_exon_Gm13802_chr2_93098149_R | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intron_Arhgap39_chr15_76781217_F | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intron_Arhgap39_chr15_76781217_R | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intron_Unc80_chr1_66657247_F | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intron_Unc80_chr1_66657247_R | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intron_Sepsecs_chr5_52664101_F | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intron_Sepsecs_chr5_52664101_R | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intron_Trabd2b_chr4_114540590_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intron_Trabd2b_chr4_114540590_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_Ern1|Snord104_chr11_106498403_F | TCGTCGGCAGCGTCCAACATTTTGGGGCCAGGTG | 59.9 | 0.07 |
| mm4_intergenic_Ern1|Snord104_chr11_106498403_R | GTCTCGTGGGCTCGGACAGCATCCCTAGGAGCTGA | 60.0 | 0.07 |
| mm4_intergenic_Igsf3|Atp1a1_chr3_101471846_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_Igsf3|Atp1a1_chr3_101471846_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intron_Acan_chr7_79109074_F | TCGTCGGCAGCGTCACACCTCTGCTTCTGCCATC | 60.0 | 0.07 |
| mm4_intron_Acan_chr7_79109074_R | GTCTCGTGGGCTCGGTGGGCCTCCTTTTCTGCATT | 59.8 | 0.07 |
| mm3_exon_Abhd16a_chr17_35102851_F | TCGTCGGCAGCGTCGGAGGAGACACGAGGGGATA | 59.8 | 0.07 |
| mm3_exon_Abhd16a_chr17_35102851_R | GTCTCGTGGGCTCGGATGAGGGAAACGTGCAGGAG | 60.0 | 0.07 |
| mm4_intron_Pih1d2_chr9_50624254_F | TCGTCGGCAGCGTCACAAGGATTGGCTGACCTCG | 60.0 | 0.06 |
| mm4_intron_Pih1d2_chr9_50624254_R | GTCTCGTGGGCTCGGCGACTTAACAGCTGACAAATGGA | 59.2 | 0.06 |
| mm4_intergenic_Asb9|Gm15216_chrX_164625286_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_Asb9|Gm15216_chrX_164625286_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_exon_Sez6l2_chr7_126963694_F | TCGTCGGCAGCGTCACCTATCAATGCGAGCCTGG | 59.8 | 0.05 |
| mm4_exon_Sez6l2_chr7_126963694_R | GTCTCGTGGGCTCGGTTAGAGGTGGGGCCCTACTC | 60.0 | 0.05 |
| mm3_exon_Pitpnm1_chr19_4113559_F | TCGTCGGCAGCGTCTTCTGCCCACAGTTCCTGTC | 59.8 | 0.05 |
| mm3_exon_Pitpnm1_chr19_4113559_R | GTCTCGTGGGCTCGGGGCAGCCACAGCATAGCTA | 59.8 | 0.05 |
| mm4_exon_Esrrg_chr1_187609064_F | TCGTCGGCAGCGTCTTATAGTCTACGCCCGGCAG | 59.0 | 0.05 |
| mm4_exon_Esrrg_chr1_187609064_R | GTCTCGTGGGCTCGGGCTGCAAGAGGGGAAAACCA | 60.8 | 0.05 |
| mm3_intergenic_Adcy9|Srl_chr16_4444567_F | TCGTCGGCAGCGTCGACCTCCTCCCCTGAAGACA | 60.2 | 0.05 |
| mm3_intergenic_Adcy9|Srl_chr16_4444567_R | GTCTCGTGGGCTCGGTGCTTTATGACCATGGTAGCA | 57.3 | 0.05 |
| mm4_intergenic_Msr1|Fgf20_chr8_39737207_F | TCGTCGGCAGCGTCTGGTCATGTGTGATGGTCCC | 59.6 | 0.05 |
| mm4_intergenic_Msr1|Fgf20_chr8_39737207_R | GTCTCGTGGGCTCGGGGGTTGAGGCACCGACTTTA | 59.9 | 0.05 |
| mm4_intergenic_Gm12437|Gm23746_chr4_48828331_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Gm12437|Gm23746_chr4_48828331_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Impad1|Gm11779_chr4_4838786_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Impad1|Gm11779_chr4_4838786_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm3_intron_Kalrn_chr16_34144538_F | TCGTCGGCAGCGTCTGAACTGGGATGCACCTGTC | 59.9 | 0.03 |
| mm3_intron_Kalrn_chr16_34144538_R | GTCTCGTGGGCTCGGGGCTTGAGAAACACGTTGCA | 59.6 | 0.03 |
| mm4_intron_Gpr158_chr2_21720398_F | TCGTCGGCAGCGTCCAGCTGGCAGGTAGTGGAAT | 59.7 | 0.03 |
| mm4_intron_Gpr158_chr2_21720398_R | GTCTCGTGGGCTCGGTCTGCTTTACCTGTCTCTCTGG | 59.1 | 0.03 |
| mm4_intron_Gpr158_chr2_21717441_F | TCGTCGGCAGCGTCTCTGCTTTACCTGTCTCTCTGG | 59.1 | 0.03 |
| mm4_intron_Gpr158_chr2_21717441_R | GTCTCGTGGGCTCGGCAGCTGGCAGGTAGTGGAAT | 59.7 | 0.03 |
| mm4_intron_Cdh23_chr10_60485602_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intron_Cdh23_chr10_60485602_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intron_1700007B14Rik_chr8_75528850_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intron_1700007B14Rik_chr8_75528850_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm3_intergenic_Gm20672|Abcg2_chr6_58574897_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm3_intergenic_Gm20672|Abcg2_chr6_58574897_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Mfsd4/Gm26892|Gm26034_chr1_132098431_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Mfsd4/Gm26892|Gm26034_chr1_132098431_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_exon_Midn_chr10_80157356_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_exon_Midn_chr10_80157356_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_A830012C17Rik|Gm11780_chr4_4419885_F | TCGTCGGCAGCGTCGGTTGTCACAGGGAGAGTCA | 58.9 | 0.02 |
| mm4_intergenic_A830012C17Rik|Gm11780_chr4_4419885_R | GTCTCGTGGGCTCGGGTGTCCAAGGCTTTCCTCTGA | 59.9 | 0.02 |
| mm4_intergenic_Gm10051|Gm15627_chr5_133675140_F | TCGTCGGCAGCGTCTGGAGACAAGCTTCCCTTTCC | 59.9 | 0.02 |
| mm4_intergenic_Gm10051|Gm15627_chr5_133675140_R | GTCTCGTGGGCTCGGGAAGATCCGCTCAGAGGGAG | 59.3 | 0.02 |
| mm4_intron_Ctcfl_chr2_173101850_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intron_Ctcfl_chr2_173101850_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intron_Plekha7_chr7_116228614_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_Plekha7_chr7_116228614_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_exon_P2rx1_chr11_72999258_F | TCGTCGGCAGCGTCCACTTGGCCTGCTCTGTTCT | 60.2 | 0.01 |
| mm4_exon_P2rx1_chr11_72999258_R | GTCTCGTGGGCTCGGGAAGGGGCTCAGAGACAAGC | 60.3 | 0.01 |
| mm4_exon_Tpsg1/Cacna1h_chr17_25375802_F | TCGTCGGCAGCGTCACACTGCAGAACCTGTGTGG | 60.4 | 0.01 |
| mm4_exon_Tpsg1/Cacna1h_chr17_25375802_R | GTCTCGTGGGCTCGGGAGGAGGTCAGCCACATCAC | 60.1 | 0.01 |
| mm3_intergenic_Zhx2|Gm16006_chr15_57701620_F | TCGTCGGCAGCGTCCTCTCCCTGACCTGGACAGA | 59.9 | 0.01 |
| mm3_intergenic_Zhx2|Gm16006_chr15_57701620_R | GTCTCGTGGGCTCGGCCCCTATGCTTGCTCCTTCC | 60.1 | 0.01 |
| mm4_intergenic_Myh9|Txn2_chr15_77876166_F | TCGTCGGCAGCGTCCCTCAGCTGCTTCAGACAGG | 60.3 | 0.01 |
| mm4_intergenic_Myh9|Txn2_chr15_77876166_R | GTCTCGTGGGCTCGGTCCCGACTGTAACATGTGGT | 58.6 | 0.01 |
| mm4_intergenic_Gm14109|Gm14107_chr2_147439934_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Gm14109|Gm14107_chr2_147439934_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm3_intergenic_Slc16a10|Rpf2_chr10_40221224_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm3_intergenic_Slc16a10|Rpf2_chr10_40221224_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm3_intergenic_Uri1|Ccne1_chr7_38074853_F | TCGTCGGCAGCGTCTCATCAAAGGCTCCATCGGG | 59.8 | 0.00 |
| mm3_intergenic_Uri1|Ccne1_chr7_38074853_R | GTCTCGTGGGCTCGGATAGAGCTTGGAGGGGTCCC | 60.4 | 0.00 |
| mm4_intron_Abcc4_chr14_118690772_F | TCGTCGGCAGCGTCTCTCAGAAGCCACTCTTGCG | 60.0 | 0.00 |
| mm4_intron_Abcc4_chr14_118690772_R | GTCTCGTGGGCTCGGGACCCGTGTTCCAACATCCA | 60.2 | 0.00 |
| mm3_exon_Rag2/B230118H07Rik_chr2_101629434_F | TCGTCGGCAGCGTCAAAATGTCCCTGCAGATGGT | 57.3 | 0.00 |
| mm3_exon_Rag2/B230118H07Rik_chr2_101629434_R | GTCTCGTGGGCTCGGACTCCAGTAGGACAGGATCTCT | 59.1 | 0.00 |
| mm4_intergenic_Ralgapb|Adig_chr2_158499851_F | TCGTCGGCAGCGTCGCATCTGAACTGCCTGGGAT | 60.1 | 0.00 |
| mm4_intergenic_Ralgapb|Adig_chr2_158499851_R | GTCTCGTGGGCTCGGAATGAGGCCATCAAGGTGGG | 60.0 | 0.00 |
| mm4_intron_Xrcc6bp1_chr10_126870557_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Xrcc6bp1_chr10_126870557_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Ptn|Dgki_chr6_36813967_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Ptn|Dgki_chr6_36813967_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Ust_chr10_8263079_F | TCGTCGGCAGCGTCAGATCAGAGAGCTGTCCGGA | 59.7 | 0.00 |
| mm4_intron_Ust_chr10_8263079_R | GTCTCGTGGGCTCGGGCAGAGCAAGATTGAGGTGC | 59.5 | 0.00 |
| mm4_intergenic_Zkscan2|Gm23788_chr7_123642086_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Zkscan2|Gm23788_chr7_123642086_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm2_exon_Prtn3_chr10_79881940_F | TCGTCGGCAGCGTCGGAAGAGACGAGTCCTCACG | 59.5 | 0.00 |
| mm2_exon_Prtn3_chr10_79881940_R | GTCTCGTGGGCTCGGGACAGAGTCTGGTCCTGCTG | 59.7 | 0.00 |
| mm4_exon_Atg7_chr6_114860266_F | TCGTCGGCAGCGTCCACTGACGGGTATCTGAGGC | 59.8 | 0.00 |
| mm4_exon_Atg7_chr6_114860266_R | GTCTCGTGGGCTCGGACTCCATGACAACACTGCGT | 59.8 | 0.00 |
| mm4_intron_Myo5c_chr9_75257158_F | TCGTCGGCAGCGTCCGTCCTAGCATAGAAGCCCC | 59.6 | 0.00 |
| mm4_intron_Myo5c_chr9_75257158_R | GTCTCGTGGGCTCGGCACAGCCCTTCCTTCTGGC | 60.6 | 0.00 |
| mm4_exon_Fam43b/AB041806_chr4_138396173_F | TCGTCGGCAGCGTCGAGAGATGCCCAGTATCCGG | 59.3 | 0.00 |
| mm4_exon_Fam43b/AB041806_chr4_138396173_R | GTCTCGTGGGCTCGGGACGGCTGGAGTCATTCCTG | 60.4 | 0.00 |
| mm4_intron_Zfp423_chr8_87916116_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Zfp423_chr8_87916116_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Sulf1_chr1_12851127_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Sulf1_chr1_12851127_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Shank2_chr7_144117087_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Shank2_chr7_144117087_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Rabgap1l_chr1_160352148_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Rabgap1l_chr1_160352148_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_n-R5s179|4930505K14Rik_chr5_148775385_F | TCGTCGGCAGCGTCCCTCAGGTGCACATAAGGCA | 60.0 | 0.00 |
| mm4_intergenic_n-R5s179|4930505K14Rik_chr5_148775385_R | GTCTCGTGGGCTCGGATCACAGGAAAGGGTGTGGC | 60.2 | 0.00 |
| mm4_intergenic_Zfp592|Alpk3_chr7_81051318_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Zfp592|Alpk3_chr7_81051318_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Samd12|Gm23200_chr15_53529912_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Samd12|Gm23200_chr15_53529912_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Kifc5c-ps|Cpm_chr10_117628818_F | TCGTCGGCAGCGTCTTTGTTTGCCCCAGACCCTT | 60.0 | 0.00 |
| mm4_intergenic_Kifc5c-ps|Cpm_chr10_117628818_R | GTCTCGTGGGCTCGGTGGTGAGAAGTGGGTTCGTG | 59.8 | 0.00 |
| mm4_intergenic_Gm7091|Vsig1_chrX_140840448_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm7091|Vsig1_chrX_140840448_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm26184|Dlgap2_chr8_14352784_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm26184|Dlgap2_chr8_14352784_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_exon_Vps9d1_chr8_123246906_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_exon_Vps9d1_chr8_123246906_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_exon_Gm16330_chr8_66588738_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_exon_Gm16330_chr8_66588738_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_exon_Dupd1_chr14_21703012_F | TCGTCGGCAGCGTCAGGCTCCAGGGGTACAGTAG | 60.0 | 0.00 |
| mm4_exon_Dupd1_chr14_21703012_R | GTCTCGTGGGCTCGGGAACCTCCCTTGGCTCCAAA | 59.8 | 0.00 |
These only list off-targets that have primers in the table above. Primers underlined, off-targets in bold.
| ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52314145 |
GTCGATGGACTAGCAGGCAGTGCTGCAGGGCTGGGCGCCGAGCCACGGTCTGCTGGAGCGGCCATGCTTGGCCCGGGACC CCCAGTCCCCTCCGCGGACAGCCTCTCTGGCCAAGGGCAACCTAGTAGCTCAGACACCGA |
| mm3_intron_Ccdc147_chr19_48001208 |
TCCTGCTGCAATGTCTCTGAGTTTCCTCGTATTGCTTTTCTTGGCTATCTAAGAGTCAAGGGAGAGAGAGAGTAGACTTT CAGGGCCAGACAGCTTCCCTGGCCAATGGACAGGAGAGGCCTCCTTGGGGTAGTGTCTGC |
| mm4_intergenic_Gm13888|AI314831_chr2_105050800 |
CCACAAGTCCAGCCCATGATAAGCCTTCTTTCACAGGGAAACCCCTGGCCAGTGTGGCTGTCTGGGATTTGTACACCTAG CACAGTCAATCTTCATTTCAGAAGCCAGAAGCCCC |
| mm4_intron_Plb1_chr5_32243424 |
AGCCCCAGAATCTTTACAGATGGTGTATTCCAGCAGATTTCTCTTTAGAAGGTCAGCCTCTTTGGCCAGTGGTTAATAGC CTGTCCTCTTGCCAGGTGGACTTT |
| mm3_intergenic_Gm6317|Gm25383_chr13_78455202 |
AAACTGGCAGGCCAACTCTGCCAGACAGCATCTCTGGACAAAGGCTTTTCCTTCCCTGGTGTCCAGTCTTTACTTGGTTT ACTCGATGCTGTGTTGGTCTTTCCTGGGCAGAGTC |
| mm4_intron_Chchd3_chr6_32995639 | CCACAGGCTAAGTCAATCCCCTTTAATGTGCCATTGGCCAGAGAACCTCTCCAGGAGTGAGTCCTTGCTGCTGT |
| mm4_intergenic_4930594M17Rik|Gm26012_chr18_81660904 |
GGACCAGGAAAGGAAGCAGGAAGAACAGCTCAGAAGCCAACCTTAGGCCAGGGAGTCTGTCCCTGGAAATGCCCTGAAAG CAGAAGAGATGGAGAGAAAGCTAACTGGATCCTCTCACAGGAAGGAGAGGCTGCAAA |
| mm4_intron_Fam47e_chr5_92568450 |
AGCATGAGGTAACGCCACCGCTCTCCCCTTGGCCAGATACTCTGTCCTGTCCTCTGAGAAGAACCCTGAAGGAGGCATCC |
| mm4_intergenic_Gm24932|Syt9_chr7_107344265 |
CCTGCTACTAGCTCCCCTCTTCTGTTTGGGAACCAACCCTGTTCCCATTGGCCAGATAGGCTGCCATCATGATGCCCGTT CCTCCTA |
| mm4_intron_Slc24a2_chr4_87077803 |
TGCATCAGGTTACGGTAGGTAAACGTAGGTGACATACCAATGGCCAGTGAGCCTGTCTGGACTGACCTGTTGCTTTGATG TAGTCTGTTGAGAGGTGTGACATGTGATAAAGACTACCTCTCACCCCGA |
| mm2_intergenic_Lingo1|Odf3l1_chr9_56792590 |
CTTCCCCACAGCATCAGGACCATCTCACAGCCTCTCTGGCCAAAGGTCCCCAGCATCAGCATCAGGCATGGTTTAAATCA CAAATCTAATTTTGTCCCTTGAGAATTTCATCCCTGACCCCTGC |
| mm4_intergenic_Adra2a|Gpam_chr19_54928476 |
ACCTGGGGATCTGCTTTGTGCATATGGAATCTGCTCTCCTCTCTGGAGATGGGAGCATCATTGTAAACCCCCTCCCAGTA CCCTTGGCCTAAGTGGCTGTCAGAAAGTTGGCCCCACCCTTTGACCCTAAG |
| mm4_exon_Kif13b_chr14_64806224 |
GAAGTGCTACCATCTCGGGCTCTGCCACCAACCTGGCTTCACTGACAGCTGCACTGGCCAAGGGAGACAGGAGTTACAAG AATCCTGAGAACCGGAAGTCCTGGGCCAGCTAAGATGCACCTCCCTTGTGG |
| mm3_intron_Usp24_chr4_106408329 |
GTGAACTGATGGCCCTGGAGGCCGTCTGGGAACGGAGAGACTCTCTGGCAAAGGGCCCAAAGGACTAGACTACTATTTGT TTTGATGCTTAGACGCTAAGATTTCTGCCTTTCTCAGTAAACAACAGACTTGCCCAGAGT |
| mm4_intergenic_Slc28a3|Ntrk2_chr13_58661382 |
GATGCGAAACCCCAAAGCAGACTTAATCCAAAGGACACTCAGCATCTTTGTAGATAAAAGTTCCAGGTCACGCCTTTAGC CAGATAGTCTGTCAGGTTCGGCCTCTTCCCCAGATCTGT |
| mm4_intergenic_Cbx2|Cbx8_chr11_119033926 |
ACCTCAGGTCAGGACAGGAACAAGTGCTGGCCCCATTGGCCAGCTAGGCTCTCTGAAAATCTATGTCTACAGGGACAAGG TCTCCAGACCAAAGTTGAGGGGGCGGGGCTGGCCTACATTGATGT |
| mm4_intron_Tmem189/Gm20431_chr2_167653105 |
AGGAGGTGACCAGCCAGTATGGCACCCAGCTGGACCTACTACAGGGTGTCTCCCCACCCTTGGCCAGGGAGGATGTGCCT AGAGAGCCCAGGGCCGGTGGGAAGAGAACAAT |
| mm4_intron_Zfp280c_chrX_48594081 |
CAAAGCCAGAGAGGTCCTGGGGCCCGGACAGCCTCCCAGGCCTTCGGAGGCCAGGCTAGCGCCCAACCCGGGCCCACGCG GCCACCCATGCCCGGTCCAGGCCTGGGAGGCAGCCGCCGGCGATCGCCACAAGCACCCC |
| mm4_intergenic_Psap|Cdh23_chr10_60302676 |
TCAGCCAGAAGAGAGTGGGTTCTGGGTCCCCTTCCCCTTCCCCTTCAGCTCCTAGCAGCCGCTCTGGCCAAGGGGCTCCC TCCCATCCCCCCAGGACCTTCATACGGCACCAAGTCAGGGTATT |
| mm4_intron_Gm872_chr10_93069427 |
AGGACACTGAACAGCCAGGCCGTTGGCCTGAGGGGCTGACAGGGCAAGGCCACAGGCCTAGGGAAAAACAACAGGAAATA GTGTCTGGGCTCCTTTGTG |
| mm4_intron_Crtac1_chr19_42401159 |
CACTGTTCTCAGGTCCGTGGGACCGGTGTCTTTAGTAGAGGTGAACAGAAAGCCTCTCTGGTAAAAGGTGTATTTAGTTA AGGCCTACATAGCTCCCACA |
| mm4_intergenic_Trib1|Gm7083_chr15_59660168 |
AGGTCCTTTCTGCTGTTGTTTTGAGATTCTGTTGGAAACAGGGCCTACCCCTTGCCAGAGAGGCTGTGGGAACAGCCTTG TGCTTCCTTTCAAAACTAGACCCGGGCCAT |
| mm3_intergenic_Sptlc1|Msx2_chr13_53430622 |
TCACACAGTTCCAGCTGTCCTTCTGCCCTTGGCCAGCCAGGCTGTCTGCAAGGTTGGAGGGATGCTCTAACGACTTGCAG AGGGAGCCTAGAAACTCCAGGCAGTTGAGCTCAGAA |
| mm4_intergenic_Gm20388/Galnt2|1810008B01Rik/Gm20388/Galnt2_chr8_124301650 |
TGTTCCTGTGTGTAGCAGGCCTATGGATATGGCTGCCAGCTGCTCTGGCCAAGGGAAGGTGATGTGGGAAGTCCTTGCAC TGAACTGGGGAGTCCAAGGATCGCAGCCAGAGGTGTGACTCATGAGCCC |
| mm4_intergenic_Ern1|Snord104_chr11_106498403 |
CAACATTTTGGGGCCAGGTGTTTTAATTTTTTTTTTAATTGTTCAGATAACCCTGGGCTATAGAGGCTGTCCTATGCATT GATGGGTGTCCCCTGACCCCTCTGCTCTCTCCCTGTACTCAGCTCCTAGGGATGCTGT |
| mm4_intron_Acan_chr7_79109074 |
ACACCTCTGCTTCTGCCATCTGTGGGCCAGGAGCCCTAGACAGGACCAGGCTGACACCCGGCACTCCACTTGTGGTGCTT TCTAAACTCCTGCCAGCCTCTCTGGCCTTGGGCATTCCCAATGCAGAAAAGGAGGCCCA |
| mm3_exon_Abhd16a_chr17_35102851 |
GGAGGAGACACGAGGGGATACTTTTGTGACTCTCTTCATGTTGCTGTTTATAGTCTGGCCCGTGGGGACATCCCCTCTGG CCAACAGTCCTCCTGCACGTTTCCCTCAT |
| mm4_intron_Pih1d2_chr9_50624254 |
ACAAGGATTGGCTGACCTCGCCCTCCGCCTGTTCACAGGCTTTTTAAACAAATGCTGTTTAACCAGTCAGCCACTGTGGC CAACGGCAATTTCTTATTTTATCCTTTCTGGATAGATTCCATTTGTCAGCTGTTAAGTCG |
| mm4_exon_Sez6l2_chr7_126963694 |
ACCTATCAATGCGAGCCTGGCTATGAACTACTGGGATCAGACATCCTCACTTGCCAATGGGACCTGTCCTGGAGCGCGGC GCCACCTGCCTGCCAAAAGAGTGAGTAGGGCCCCACCTCTAA |
| mm3_exon_Pitpnm1_chr19_4113559 |
TTCTGCCCACAGTTCCTGTCAGATGGCTATGTGGCCCACTTGGGCCAGCTGGAGGCAGGCTCCCACTCCCATGCTCCCTC AGGACCTCCGAGAGCCGCTCTGGCCAAGAGTAGCTATGCTGTGGCTGCC |
| mm4_exon_Esrrg_chr1_187609064 |
TTATAGTCTACGCCCGGCAGCCCGAACCCCGTGCCCGATTCCTGGTGCGGAGTGCGAGAGGTTCCCGCGGCGCCTGGCGG ACAGTCTCGCTGGCCTCCGGTGACTTGTTTTGTGTTGGTTTTCCCCTCTTGCAGC |
| mm3_intergenic_Adcy9|Srl_chr16_4444567 |
GACCTCCTCCCCTGAAGACATATCTGTGGAAATTTCCACCAGCAAAATCCTGGAAACATTGCTCACACAGCCTCTCTGGC CATGAGCCAGACATCTTTCTTCAGTGATTATGCTACCATGGTCATAAAGCA |
| mm4_intergenic_Msr1|Fgf20_chr8_39737207 |
TGGTCATGTGTGATGGTCCCTCCACCCCCTGCATATAACATTTCTTTCGCTACATTTTCTTTTGGATAAAGAATAGTGAC ACTGTCAGCCTCTCTGGCCTCAGGGACTAAAGTCGGTGCCTCAACCC |
| mm3_intron_Kalrn_chr16_34144538 |
TGAACTGGGATGCACCTGTCCCATGTAACCTTTGCCCAGAGAGGCTGACCAATTTCATGCGCAGTTGTGGCAGGCAACCT CCTAGACTTAGTCTTGCAACGTGTTTCTCAAGCC |
| mm4_intron_Gpr158_chr2_21720398 | CAGCTGGCAGGTAGTGGAATACCTGTGGCCAGAGAGGCTTTGGGGATTTCTACCAGAGAGACAGGTAAAGCAGA |
| mm4_intron_Gpr158_chr2_21717441 | TCTGCTTTACCTGTCTCTCTGGTAGAAATCCCCAAAGCCTCTCTGGCCACAGGTATTCCACTACCTGCCAGCTG |
| mm4_intergenic_A830012C17Rik|Gm11780_chr4_4419885 |
GGTTGTCACAGGGAGAGTCAAATCATTGAGAGAATGTAGTAAACTTTCTTGTAGAAGACTGATAGTCACTCTGGCCAAAG AACCATCCATTCAGTAATGGGAATTGATAGTCTGAATCAGAGGAAAGCCTTGGACAC |
| mm4_intergenic_Gm10051|Gm15627_chr5_133675140 | TGGAGACAAGCTTCCCTTTCCCCTTTGCCCAGAGAGGAAGTCTGCTCCCTCTGAGCGGATCTTC |
| mm4_exon_P2rx1_chr11_72999258 |
CACTTGGCCTGCTCTGTTCTTAAGGGGCTGGGAAGCCTCCAGCCACTCCACTGCTCTCTCTGTAGACACAGATGCTAGCC TGCCCTTGACCACAGAGGCCGTGCGGGGGGCTTGTCTCTGAGCCCCTTC |
| mm4_exon_Tpsg1/Cacna1h_chr17_25375802 |
ACACTGCAGAACCTGTGTGGGTCCCGCCCACTGCCCACTGTGCCTTTTGCAGGAGAGGCTGTCGGGGAGGCCTCTGGGCT GTGGGGCTCTGTAGCTGGCCAAGGGTGGGCTGAGCTGGTGATGTGGCTGACCTCCTC |
| mm3_intergenic_Zhx2|Gm16006_chr15_57701620 |
CTCTCCCTGACCTGGACAGAAAGAATCCCAGGGAGCCAAAAGCTGTAGAATTGGGACCCGAAGAGGAGCTTGGGCCTTCA GGATGCAGCACAGCCTCGCTGGCCAATGAGGAGCAGGGGTGGAAGGAGCAAGCATAGGGG |
| mm4_intergenic_Myh9|Txn2_chr15_77876166 |
CCTCAGCTGCTTCAGACAGGATTGCTATCTGATGAGGACAAGCCCTTGGCCACAAAGGCTGTAAGTCATGCCCTTACTAT AAACAGAGTTTATAGTCCAGCTTGTGAGATTCCTAGGAAACCACATGTTACAGTCGGGA |
| mm3_intergenic_Uri1|Ccne1_chr7_38074853 |
TCATCAAAGGCTCCATCGGGCTGCACGCGCCCCGGCCCTGTCCTCCTGGCCGCCGCCTTTGTACTCACTCCTCGGCCAGA GAGGCTGGCCACCTCCCTCTGACTGTGCGCTCCAAACGCAGGGACCCCTCCAAGCTCTAT |
| mm4_intron_Abcc4_chr14_118690772 |
TCTCAGAAGCCACTCTTGCGGGGTAAGACACCTCTGCCTTTGGAGAAAAGGTGACCCACAACCTACAGCCCAGACAGCCT CACTGGGCACGGGTTTTTGTGGATGTTGGAACACGGGTC |
| mm3_exon_Rag2/B230118H07Rik_chr2_101629434 |
AAAATGTCCCTGCAGATGGTAACAGTGGGTCATAACATAGCCTTAATTCAACCAGGCTTCTCACTTATGAATTTTGATGG CCAAGTTTTCTTCTTTGGCCAGAAAGGCTGGCCTAAGAGATCCTGTCCTACTGGAGT |
| mm4_intergenic_Ralgapb|Adig_chr2_158499851 |
GCATCTGAACTGCCTGGGATCCAGAGCCACCTTGTGCATTCTTCTTACATCAGCTCTACTTGCCAGCCACAGGACCTCCA GCCAGGCCCTCTGGCCAAAGAAAGCCCACCTTGATGGCCTCATT |
| mm4_intron_Ust_chr10_8263079 |
AGATCAGAGAGCTGTCCGGAAGCCCACCATCCAGTCAGCCTCTCTGGGGAATGGTTTCTTTGGCACCTCAATCTTGCTCT GC |
| mm2_exon_Prtn3_chr10_79881940 |
GGAAGAGACGAGTCCTCACGTCCTTTTTTCCTATAGCTAAACCGGACAGCCTCCCTGGGCAAGGAGGTGGCGGTGGCTTC TCTGCCCCAGCAGGACCAGACTCTGTC |
| mm4_exon_Atg7_chr6_114860266 |
CACTGACGGGTATCTGAGGCCCGTTGCCCACAGAGCCAGTCCGCGGTGCGGTTGTTTTCCTGTGACAACCTCAGTAGCCT GTAGAATAACAAACTAGTGGCTATTAATGCGGACGCAGTGTTGTCATGGAGT |
| mm4_intron_Myo5c_chr9_75257158 |
CGTCCTAGCATAGAAGCCCCTCCAACTCAATCTTTTTACTTTAGGTGTTTTCGTCCTTTCTTAGACAGAAAGGCTCTGCC AATCCCTTCCCCAGAGAGGGTGTCAGGCGGGGCCAGAAGGAAGGGCTGTG |
| mm4_exon_Fam43b/AB041806_chr4_138396173 |
GAGAGATGCCCAGTATCCGGCGGGGCTGGCTTCGCAGATGCCCGGTGCCCAGCCAGGCTGTCCGCTGAGATGCCCGTGCG GCTGCTTGATCGGCTCGGAGCAGCTTCCCCCAGGAATGACTCCAGCCGTC |
| mm4_intergenic_n-R5s179|4930505K14Rik_chr5_148775385 |
CCTCAGGTGCACATAAGGCAGAGAGTCAAGAGCTTAAGGAGATTTTTTTTTTTGAAACTAGAACTCTAGGCTGTCCCTAG GGCAGAGAGACTGTCTGGTCTTGATCACTTTATCTGGGCCACACCCTTTCCTGTGAT |
| mm4_intergenic_Kifc5c-ps|Cpm_chr10_117628818 |
TTTGTTTGCCCCAGACCCTTGCAGGAGCCGGTGTTTCTTTAACAGTGTATGTAAATCCATGGGACAGAGAGACTGTGCGT GTGGTGCCCTTTAGAACATGATGGCCCCTTGTCACGAACCCACTTCTCACCA |
| mm4_exon_Dupd1_chr14_21703012 |
AGGCTCCAGGGGTACAGTAGTCCTCAGCATCTCCCTCCTCCTCCCGCACGGACAGCCTCTCAGCACAGAGGTGGGCATGT TTGACGCTTGTCTTTGTATCTCCTGATGCCATTTTGGAGCCAAGGGAGGTTC |