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Contents:In the table below, Illumina Nextera Handle sequences have been added and highlighted in bold. Primers for the on-target have been added for convenience. The table below is sorted by the CFD off-target score. Sites with very low CFD scores < 0.02 are unlikely to be cleaved, see our study Haeussler et al. 2016, Figure 2.
In the protocol by Matthew Canver, Harvard, two PCRs are run: one PCR to amplify the potential off-target, then a second PCR to extend the handles with Illumina barcodes. Please click here to download the protocol. Alternatively, you can have a look at Fu et al, 2014.
If a primer was not found, the reason is usually that the region around the off-target is too repetitive. To avoid unspecific primers, all repeats are masked for the primer design (not for off-target search). If you think that we should change the parameters here or should use different primer3 settings, please let us know.
| Name | Primer Sequence | Tm | CFD Score |
|---|---|---|---|
| ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52313632_F | TCGTCGGCAGCGTCTTGCAGCAGGAGGATGACAG | 60.0 | 1.00 |
| ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52313632_R | GTCTCGTGGGCTCGGAGATGGCAGTGGGGAGCTA | 59.9 | 1.00 |
| mm4_exon_Pcdhb9_chr18_37401631_F | TCGTCGGCAGCGTCATACACAGTCAGCCCCAACC | 59.6 | 0.56 |
| mm4_exon_Pcdhb9_chr18_37401631_R | GTCTCGTGGGCTCGGGGTTAAGCTCAGCTCTGCCT | 59.7 | 0.56 |
| mm4_exon_Pcdhb7_chr18_37342477_F | TCGTCGGCAGCGTCCACAGTCAGCCCCAACTCTC | 60.3 | 0.56 |
| mm4_exon_Pcdhb7_chr18_37342477_R | GTCTCGTGGGCTCGGGGTTAAGCTCAGCTCTGCCT | 59.7 | 0.56 |
| mm4_intron_Blk_chr14_63413701_F | TCGTCGGCAGCGTCGAAGCAGAGCACAAACAGGC | 60.0 | 0.49 |
| mm4_intron_Blk_chr14_63413701_R | GTCTCGTGGGCTCGGGGAAGTGGCACTGCGAATTT | 59.4 | 0.49 |
| mm4_intergenic_Stk25|Bok_chr1_93666644_F | Primer3: not found at this Tm | N.d. | 0.40 |
| mm4_intergenic_Stk25|Bok_chr1_93666644_R | Primer3: not found at this Tm | N.d. | 0.40 |
| mm4_intergenic_n-R5s82|Pknox2_chr9_37075456_F | Primer3: not found at this Tm | N.d. | 0.38 |
| mm4_intergenic_n-R5s82|Pknox2_chr9_37075456_R | Primer3: not found at this Tm | N.d. | 0.38 |
| mm4_intergenic_S100a4|S100a5_chr3_90606365_F | Primer3: not found at this Tm | N.d. | 0.34 |
| mm4_intergenic_S100a4|S100a5_chr3_90606365_R | Primer3: not found at this Tm | N.d. | 0.34 |
| mm4_intergenic_Klhl25|Gm5597_chr7_76095928_F | Primer3: not found at this Tm | N.d. | 0.29 |
| mm4_intergenic_Klhl25|Gm5597_chr7_76095928_R | Primer3: not found at this Tm | N.d. | 0.29 |
| mm3_intergenic_Gm22807|Gm23681_chr11_20912209_F | Primer3: not found at this Tm | N.d. | 0.26 |
| mm3_intergenic_Gm22807|Gm23681_chr11_20912209_R | Primer3: not found at this Tm | N.d. | 0.26 |
| mm4_exon_Gpr26_chr7_131966615_F | TCGTCGGCAGCGTCAAAGCCAGTGACCACAGGAG | 59.8 | 0.26 |
| mm4_exon_Gpr26_chr7_131966615_R | GTCTCGTGGGCTCGGCGATCACCTAGGCGAGTGTT | 59.5 | 0.26 |
| mm4_intron_Dock2_chr11_34724421_F | Primer3: not found at this Tm | N.d. | 0.24 |
| mm4_intron_Dock2_chr11_34724421_R | Primer3: not found at this Tm | N.d. | 0.24 |
| mm4_intron_Grid2_chr6_64083737_F | Primer3: not found at this Tm | N.d. | 0.23 |
| mm4_intron_Grid2_chr6_64083737_R | Primer3: not found at this Tm | N.d. | 0.23 |
| mm4_exon_Dcun1d4_chr5_73491429_F | TCGTCGGCAGCGTCGGGGACATTCTGGAAGGCC | 60.0 | 0.22 |
| mm4_exon_Dcun1d4_chr5_73491429_R | GTCTCGTGGGCTCGGCAAGCCTCACCCTGTACTCG | 60.1 | 0.22 |
| mm4_intergenic_Gm24467|Gm13456_chr2_40334846_F | TCGTCGGCAGCGTCTGCTCTCTCTGTCAGTTTTCTT | 57.3 | 0.21 |
| mm4_intergenic_Gm24467|Gm13456_chr2_40334846_R | GTCTCGTGGGCTCGGAGGGGATTTATGGGCAGCAA | 59.3 | 0.21 |
| mm4_intergenic_Gm4262|Litaf_chr16_11026824_F | Primer3: not found at this Tm | N.d. | 0.21 |
| mm4_intergenic_Gm4262|Litaf_chr16_11026824_R | Primer3: not found at this Tm | N.d. | 0.21 |
| mm4_intron_Mlh3_chr12_85252758_F | Primer3: not found at this Tm | N.d. | 0.20 |
| mm4_intron_Mlh3_chr12_85252758_R | Primer3: not found at this Tm | N.d. | 0.20 |
| mm4_intergenic_Tsen54|Llgl2_chr11_115823767_F | TCGTCGGCAGCGTCTCTCTCGCTTGGCTCTTGTG | 60.0 | 0.19 |
| mm4_intergenic_Tsen54|Llgl2_chr11_115823767_R | GTCTCGTGGGCTCGGCTGGTCACAGGACTGCTCAC | 60.3 | 0.19 |
| mm4_intergenic_Pdcd5|Dpy19l3_chr7_35652045_F | Primer3: not found at this Tm | N.d. | 0.19 |
| mm4_intergenic_Pdcd5|Dpy19l3_chr7_35652045_R | Primer3: not found at this Tm | N.d. | 0.19 |
| mm4_intron_Dpp10_chr1_123632814_F | TCGTCGGCAGCGTCTCATACCCAGCACTCAGGGA | 59.9 | 0.18 |
| mm4_intron_Dpp10_chr1_123632814_R | GTCTCGTGGGCTCGGTGAGCAAATAAACAAACATGGGAGA | 59.4 | 0.18 |
| mm4_exon_Srebf2_chr15_82192224_F | Primer3: not found at this Tm | N.d. | 0.18 |
| mm4_exon_Srebf2_chr15_82192224_R | Primer3: not found at this Tm | N.d. | 0.18 |
| mm4_intergenic_mmu-mir-7052/Josd2|Josd2_chr7_44470549_F | TCGTCGGCAGCGTCGTCAACGTGATCATGGCTGC | 59.9 | 0.18 |
| mm4_intergenic_mmu-mir-7052/Josd2|Josd2_chr7_44470549_R | GTCTCGTGGGCTCGGCCCTCTGTGTCATCCCACAC | 60.0 | 0.18 |
| mm4_intron_Cdk14_chr5_5380518_F | Primer3: not found at this Tm | N.d. | 0.17 |
| mm4_intron_Cdk14_chr5_5380518_R | Primer3: not found at this Tm | N.d. | 0.17 |
| mm3_exon_Hk3_chr13_55012247_F | TCGTCGGCAGCGTCACCAGTTCAGCATCTGAGGC | 60.0 | 0.17 |
| mm3_exon_Hk3_chr13_55012247_R | GTCTCGTGGGCTCGGACCACCCATGTGTCTTCACC | 59.8 | 0.17 |
| mm4_intron_Ctbp2_chr7_133037473_F | TCGTCGGCAGCGTCTAGAGGGCCAACAAAACCCC | 59.8 | 0.17 |
| mm4_intron_Ctbp2_chr7_133037473_R | GTCTCGTGGGCTCGGGACCCATTGACCCTCTGTGG | 60.0 | 0.17 |
| mm4_intergenic_4930570N19Rik/Arf4|Arf4_chr14_26646432_F | Primer3: not found at this Tm | N.d. | 0.16 |
| mm4_intergenic_4930570N19Rik/Arf4|Arf4_chr14_26646432_R | Primer3: not found at this Tm | N.d. | 0.16 |
| mm4_intergenic_Tpd52|Zbtb10_chr3_9098781_F | TCGTCGGCAGCGTCCTGCTCACAAAATGCGTGCT | 60.0 | 0.16 |
| mm4_intergenic_Tpd52|Zbtb10_chr3_9098781_R | GTCTCGTGGGCTCGGGCAACAGCGACAACAAAAGC | 59.4 | 0.16 |
| mm3_intergenic_Zfp619|Gm26526/Gm2058_chr7_39552743_F | Primer3: not found at this Tm | N.d. | 0.16 |
| mm3_intergenic_Zfp619|Gm26526/Gm2058_chr7_39552743_R | Primer3: not found at this Tm | N.d. | 0.16 |
| mm4_intergenic_Gm5627|Ryk_chr9_102773850_F | Primer3: not found at this Tm | N.d. | 0.15 |
| mm4_intergenic_Gm5627|Ryk_chr9_102773850_R | Primer3: not found at this Tm | N.d. | 0.15 |
| mm4_exon_Fam229b_chr10_39120980_F | Primer3: not found at this Tm | N.d. | 0.15 |
| mm4_exon_Fam229b_chr10_39120980_R | Primer3: not found at this Tm | N.d. | 0.15 |
| mm4_exon_Mettl24_chr10_40683409_F | TCGTCGGCAGCGTCCACTGTCAAGCCTCCCGG | 60.0 | 0.15 |
| mm4_exon_Mettl24_chr10_40683409_R | GTCTCGTGGGCTCGGCAACTCCATGCAAAGCCGTAG | 59.8 | 0.15 |
| mm4_intergenic_n-R5s21|Cpn1_chr19_43939854_F | TCGTCGGCAGCGTCGGGGCTCAGTTCTTCAACTCA | 59.9 | 0.14 |
| mm4_intergenic_n-R5s21|Cpn1_chr19_43939854_R | GTCTCGTGGGCTCGGCAGCCCTAGGGACCGTCC | 61.1 | 0.14 |
| mm4_intergenic_Gm26603|Fmnl2_chr2_52942306_F | Primer3: not found at this Tm | N.d. | 0.14 |
| mm4_intergenic_Gm26603|Fmnl2_chr2_52942306_R | Primer3: not found at this Tm | N.d. | 0.14 |
| mm4_intron_2900026A02Rik_chr5_113104447_F | TCGTCGGCAGCGTCGTCTTGGTACCCTGGCTCTG | 59.7 | 0.14 |
| mm4_intron_2900026A02Rik_chr5_113104447_R | GTCTCGTGGGCTCGGTTCCTCCTCTGCAGGCAAAG | 59.9 | 0.14 |
| mm4_intergenic_Npy2r|Gm22038_chr3_82787231_F | TCGTCGGCAGCGTCGCCCATGCCTCTGTGTTACT | 60.0 | 0.11 |
| mm4_intergenic_Npy2r|Gm22038_chr3_82787231_R | GTCTCGTGGGCTCGGTCCAGTAAGCCCTTAAAATGTACT | 57.6 | 0.11 |
| mm4_exon_Mast3_chr8_70783782_F | TCGTCGGCAGCGTCCATTCGTCTCGTCGTCCTCG | 60.5 | 0.11 |
| mm4_exon_Mast3_chr8_70783782_R | GTCTCGTGGGCTCGGGCTGGGCCATTCTCCTGTAA | 59.7 | 0.11 |
| mm4_intron_Tcof1_chr18_60824477_F | TCGTCGGCAGCGTCGCCTGCAGTGCTTTACCAAG | 59.7 | 0.10 |
| mm4_intron_Tcof1_chr18_60824477_R | GTCTCGTGGGCTCGGCTGTTTGGCAGGGTAGGGAG | 60.0 | 0.10 |
| mm4_exon_Lpcat4_chr2_112243848_F | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_exon_Lpcat4_chr2_112243848_R | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_exon_Gpr173_chrX_152346508_F | TCGTCGGCAGCGTCCAGGAAGCAGACGATTGGGT | 60.0 | 0.10 |
| mm4_exon_Gpr173_chrX_152346508_R | GTCTCGTGGGCTCGGTGCTACTGGCGAGTGTTTGT | 59.8 | 0.10 |
| mm4_exon_Rbmxl2_chr7_107209441_F | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_exon_Rbmxl2_chr7_107209441_R | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intergenic_Emx2|Gm17203_chr19_59699191_F | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intergenic_Emx2|Gm17203_chr19_59699191_R | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intron_Dnm1_chr2_32343696_F | TCGTCGGCAGCGTCGGAGGACCCCTAAACTTCAGC | 60.0 | 0.09 |
| mm4_intron_Dnm1_chr2_32343696_R | GTCTCGTGGGCTCGGTTCATGGTGCCTCAGTCAGG | 59.6 | 0.09 |
| mm4_intron_Map2k7_chr8_4242432_F | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intron_Map2k7_chr8_4242432_R | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intergenic_Gm21893|Tead4_chr6_128295978_F | TCGTCGGCAGCGTCATGAAGGAGAAGCCCAGTGC | 60.0 | 0.08 |
| mm4_intergenic_Gm21893|Tead4_chr6_128295978_R | GTCTCGTGGGCTCGGCATAGGACCACTGCCAGGAC | 59.8 | 0.08 |
| mm4_exon_Pcdhb8_chr18_37355842_F | TCGTCGGCAGCGTCCACAGTCAGTCCCAACCTCC | 59.9 | 0.08 |
| mm4_exon_Pcdhb8_chr18_37355842_R | GTCTCGTGGGCTCGGAAGTGAGTTCAGGCTGCTCC | 59.9 | 0.08 |
| mm4_exon_Pcdhb6_chr18_37334599_F | TCGTCGGCAGCGTCCACAGTCAGTCCCAACCTCC | 59.9 | 0.08 |
| mm4_exon_Pcdhb6_chr18_37334599_R | GTCTCGTGGGCTCGGAAGTGAGTTCAGGCTGCTCC | 59.9 | 0.08 |
| mm4_exon_Pcdhb4_chr18_37308210_F | TCGTCGGCAGCGTCCTCTCAGTCGCTCAGATGGC | 60.2 | 0.08 |
| mm4_exon_Pcdhb4_chr18_37308210_R | GTCTCGTGGGCTCGGGTCATCCCAGACCTTCGTGG | 60.1 | 0.08 |
| mm4_exon_Pcdhb12_chr18_37436353_F | TCGTCGGCAGCGTCCTCTCAGTCGCTCAGATGGC | 60.2 | 0.08 |
| mm4_exon_Pcdhb12_chr18_37436353_R | GTCTCGTGGGCTCGGGATCCGCCATCAAGAGCAGT | 60.1 | 0.08 |
| mm4_exon_Pcdhb11_chr18_37422190_F | TCGTCGGCAGCGTCGTCGCTCAGATGGCAGGAAA | 60.3 | 0.08 |
| mm4_exon_Pcdhb11_chr18_37422190_R | GTCTCGTGGGCTCGGCATCCAGAGCGGTGAGGATT | 59.5 | 0.08 |
| mm4_exon_Pcdhb10_chr18_37412444_F | TCGTCGGCAGCGTCCACAGTCAGTCCCAACCTCC | 59.9 | 0.08 |
| mm4_exon_Pcdhb10_chr18_37412444_R | GTCTCGTGGGCTCGGAAGTGAGTTCAGGCTGCTCC | 59.9 | 0.08 |
| mm4_intergenic_Rassf10|Gm23662_chr7_113071728_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_Rassf10|Gm23662_chr7_113071728_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_Gm7803|Gm14611_chrX_50341691_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_Gm7803|Gm14611_chrX_50341691_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_AC132241.1|Gm9898_chr1_30570190_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_AC132241.1|Gm9898_chr1_30570190_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_Gm3833|Fhit_chr14_10278289_F | TCGTCGGCAGCGTCTTGGTTGCTTGCTGGCATTC | 59.9 | 0.07 |
| mm4_intergenic_Gm3833|Fhit_chr14_10278289_R | GTCTCGTGGGCTCGGGGGGAAAAGCTGCAAATCATAA | 57.5 | 0.07 |
| mm4_intergenic_Opa1|Gm26834_chr16_29727971_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_Opa1|Gm26834_chr16_29727971_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_Max|Gm22696_chr12_76984592_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_Max|Gm22696_chr12_76984592_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_1810011O10Rik/Gm26714|Ido2_chr8_24518288_F | TCGTCGGCAGCGTCCAATTCCTCCGCCATCACCT | 60.1 | 0.06 |
| mm4_intergenic_1810011O10Rik/Gm26714|Ido2_chr8_24518288_R | GTCTCGTGGGCTCGGCTGTGAGATCACCTGCAGGG | 60.1 | 0.06 |
| mm4_intron_AI314180_chr4_58872884_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intron_AI314180_chr4_58872884_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intron_Adam5_chr8_24742843_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intron_Adam5_chr8_24742843_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_exon_Ces5a_chr8_93534582_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_exon_Ces5a_chr8_93534582_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_exon_Nupl1_chr14_60251392_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_exon_Nupl1_chr14_60251392_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_Gm14947|Gm14940_chrX_122606496_F | TCGTCGGCAGCGTCGGCTCATACCTAGACCCATTGG | 59.9 | 0.05 |
| mm4_intergenic_Gm14947|Gm14940_chrX_122606496_R | GTCTCGTGGGCTCGGCCAAGGAGCCAACAGGTAGG | 60.0 | 0.05 |
| mm4_intergenic_Unc5cl|Gm24071_chr17_48551292_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Unc5cl|Gm24071_chr17_48551292_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Abca4|Gm4609_chr3_122189973_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Abca4|Gm4609_chr3_122189973_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intron_Lsm6_chr8_78809673_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intron_Lsm6_chr8_78809673_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intron_Nsmce4a_chr7_130560758_F | TCGTCGGCAGCGTCCAGACTCCAGCAAGAGAGACA | 59.1 | 0.04 |
| mm4_intron_Nsmce4a_chr7_130560758_R | GTCTCGTGGGCTCGGCCCCACCTTGCTCTTGGAAA | 60.1 | 0.04 |
| mm4_intergenic_Gm14005|Gm14006_chr2_128536680_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Gm14005|Gm14006_chr2_128536680_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Sema6d|Gm13994_chr2_124671508_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Sema6d|Gm13994_chr2_124671508_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Gm10067|Lig4_chr8_9923230_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Gm10067|Lig4_chr8_9923230_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Gm26969|Dleu7_chr14_62251265_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Gm26969|Dleu7_chr14_62251265_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Mir5112|4930445N18Rik_chr18_82850129_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Mir5112|4930445N18Rik_chr18_82850129_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Capn10|Gpr35_chr1_92958072_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Capn10|Gpr35_chr1_92958072_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_exon_Hddc2_chr10_31313506_F | TCGTCGGCAGCGTCTAGTTGGGCAGCTCAAGGTG | 59.9 | 0.04 |
| mm4_exon_Hddc2_chr10_31313506_R | GTCTCGTGGGCTCGGGGGAGGTTGCGTGAGGTC | 60.0 | 0.04 |
| mm4_intergenic_A530013C23Rik|Gm14321_chr2_167769918_F | TCGTCGGCAGCGTCGGAGACACAGCCTGGAGAAG | 59.7 | 0.04 |
| mm4_intergenic_A530013C23Rik|Gm14321_chr2_167769918_R | GTCTCGTGGGCTCGGCCGAACAGGAACCTCACCAA | 59.8 | 0.04 |
| mm4_intron_Nsf_chr11_103829528_F | TCGTCGGCAGCGTCGGCAAGCTCCTGAGTGACTT | 59.9 | 0.04 |
| mm4_intron_Nsf_chr11_103829528_R | GTCTCGTGGGCTCGGATTTTCCTCTGGCAGACCCC | 59.6 | 0.04 |
| mm4_intergenic_Gm5432|Lpin1_chr12_16491463_F | TCGTCGGCAGCGTCCCTTGGGCGATGTTCTCAGA | 59.7 | 0.04 |
| mm4_intergenic_Gm5432|Lpin1_chr12_16491463_R | GTCTCGTGGGCTCGGTGGCACACACCTTGAATTCTG | 59.0 | 0.04 |
| mm4_intergenic_Ptchd1|Gm15155_chrX_155624149_F | TCGTCGGCAGCGTCGGAAAGACGCAGGCTGAAGA | 60.3 | 0.03 |
| mm4_intergenic_Ptchd1|Gm15155_chrX_155624149_R | GTCTCGTGGGCTCGGCTTGGGAGGAGGCGTGATTT | 60.0 | 0.03 |
| mm4_exon_Tle3_chr9_61372947_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_exon_Tle3_chr9_61372947_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intron_Gse1_chr8_120296593_F | TCGTCGGCAGCGTCCATTTGGGCTTTGGGCTGTC | 60.0 | 0.03 |
| mm4_intron_Gse1_chr8_120296593_R | GTCTCGTGGGCTCGGAACCCATCGCTACCAAGTGG | 60.0 | 0.03 |
| mm4_exon_Angptl4_chr17_33778434_F | TCGTCGGCAGCGTCCAGAGGCTGGATCTGGAAAAGT | 60.0 | 0.03 |
| mm4_exon_Angptl4_chr17_33778434_R | GTCTCGTGGGCTCGGTTGTCTACTTTCAGGGCCGC | 60.3 | 0.03 |
| mm4_intron_Lmx1b_chr2_33601332_F | TCGTCGGCAGCGTCTGAAAGCCTGTCTGCCTCTG | 59.9 | 0.03 |
| mm4_intron_Lmx1b_chr2_33601332_R | GTCTCGTGGGCTCGGTCCTGCTTTACGGTGCACTT | 59.8 | 0.03 |
| mm4_intron_Cmip_chr8_117281825_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intron_Cmip_chr8_117281825_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Gm15426|Pde10a_chr17_8801103_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Gm15426|Pde10a_chr17_8801103_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intron_Tango6_chr8_106749821_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intron_Tango6_chr8_106749821_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intron_Lmx1b_chr2_33593734_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intron_Lmx1b_chr2_33593734_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm3_intergenic_Tbc1d22a|Gm23416_chr15_86554626_F | TCGTCGGCAGCGTCCAGATCTCAGACTTGGCAGGT | 59.4 | 0.02 |
| mm3_intergenic_Tbc1d22a|Gm23416_chr15_86554626_R | GTCTCGTGGGCTCGGGCCTTCCTCCCACTGAGACT | 60.9 | 0.02 |
| mm4_intergenic_Gm6569/Mroh5|Gm7935_chr15_74048707_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Gm6569/Mroh5|Gm7935_chr15_74048707_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intron_Dock9_chr14_121718482_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intron_Dock9_chr14_121718482_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Uimc1|Zfp346_chr13_55105173_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Uimc1|Zfp346_chr13_55105173_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Lrpap1|Adra2c_chr5_35213675_F | TCGTCGGCAGCGTCAGAGACGTAGAGGGGTGCAT | 60.0 | 0.02 |
| mm4_intergenic_Lrpap1|Adra2c_chr5_35213675_R | GTCTCGTGGGCTCGGCTGAGGCTCTCCCAAGCTTC | 60.1 | 0.02 |
| mm4_intergenic_Myh7|Ngdn_chr14_55007234_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Myh7|Ngdn_chr14_55007234_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intron_Gm20388/Galnt2_chr8_124332178_F | TCGTCGGCAGCGTCAAAGTAAGTGCGGCCTTTGC | 59.6 | 0.02 |
| mm4_intron_Gm20388/Galnt2_chr8_124332178_R | GTCTCGTGGGCTCGGATGTTGGCCTTGAAGGGAGG | 59.9 | 0.02 |
| mm4_intron_Fam65c_chr2_167981235_F | TCGTCGGCAGCGTCCAGCTGAGGTAGTCTTCCCC | 59.1 | 0.02 |
| mm4_intron_Fam65c_chr2_167981235_R | GTCTCGTGGGCTCGGGTGTCAGTCTGACCTGGAGG | 59.3 | 0.02 |
| mm4_intron_Ryr1_chr7_29075548_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_Ryr1_chr7_29075548_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Actrt2|Gm13113_chr4_154695068_F | TCGTCGGCAGCGTCCACAGTAGAAGGGACCAGCG | 60.1 | 0.01 |
| mm4_intergenic_Actrt2|Gm13113_chr4_154695068_R | GTCTCGTGGGCTCGGGCAGTTGTGAAGGCCTTTGG | 59.9 | 0.01 |
| mm4_intergenic_A930031H19Rik|Gm25261_chr4_131161047_F | TCGTCGGCAGCGTCGTGGCATTCTGTCCTCTGCT | 60.0 | 0.01 |
| mm4_intergenic_A930031H19Rik|Gm25261_chr4_131161047_R | GTCTCGTGGGCTCGGGTGGGTTTCTGGAGTGAGGG | 59.9 | 0.01 |
| mm4_intergenic_Gm17388|Htr1d_chr4_136408017_F | TCGTCGGCAGCGTCTACTCCGTGAAGCTGCCAAG | 60.0 | 0.01 |
| mm4_intergenic_Gm17388|Htr1d_chr4_136408017_R | GTCTCGTGGGCTCGGCCAACTGTGCGATGCTTTCC | 60.1 | 0.01 |
| mm4_intron_Sema4a_chr3_88458941_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_Sema4a_chr3_88458941_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Gramd1b|Gramd1b/Gm17540_chr9_40473337_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Gramd1b|Gramd1b/Gm17540_chr9_40473337_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Gm22669|AC102758.1_chr7_115127676_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Gm22669|AC102758.1_chr7_115127676_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Gm22283|Gm9008_chr6_76333130_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Gm22283|Gm9008_chr6_76333130_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_Dscam_chr16_96934933_F | TCGTCGGCAGCGTCGGCTCTGTCACACACCATCA | 59.9 | 0.01 |
| mm4_intron_Dscam_chr16_96934933_R | GTCTCGTGGGCTCGGGGGAGGTGTGTGGTAGAAGC | 60.0 | 0.01 |
| mm4_intergenic_Prl4a1|Prl5a1_chr13_28115842_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Prl4a1|Prl5a1_chr13_28115842_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Msr1|Fgf20_chr8_39814338_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Msr1|Fgf20_chr8_39814338_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_A230028O05Rik|Tprg_chr16_25254841_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_A230028O05Rik|Tprg_chr16_25254841_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Slc1a2|Cd44_chr2_102795336_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Slc1a2|Cd44_chr2_102795336_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Ube2nl|Gm25533_chr7_61631474_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Ube2nl|Gm25533_chr7_61631474_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Ncr1|Gp6_chr7_4350212_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Ncr1|Gp6_chr7_4350212_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Neurl1a_chr19_47193026_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Neurl1a_chr19_47193026_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Gm26510_chr17_70892388_F | TCGTCGGCAGCGTCAGTGCTCCTGATTGTGCCTG | 60.3 | 0.00 |
| mm4_intron_Gm26510_chr17_70892388_R | GTCTCGTGGGCTCGGAAAAGCGCTCAGTCAACTGG | 59.0 | 0.00 |
| mm4_intergenic_Med27|Gm24976_chr2_29434325_F | TCGTCGGCAGCGTCCCTGCTGAGGATGAAGCTCC | 60.1 | 0.00 |
| mm4_intergenic_Med27|Gm24976_chr2_29434325_R | GTCTCGTGGGCTCGGACCACAGTCAGGAGACCCTT | 60.1 | 0.00 |
| mm4_intergenic_Slc25a36|Rpl7a-ps10_chr9_97146518_F | TCGTCGGCAGCGTCAGTCAATGTGAGGTCCTGCT | 58.6 | 0.00 |
| mm4_intergenic_Slc25a36|Rpl7a-ps10_chr9_97146518_R | GTCTCGTGGGCTCGGTGACAAGAGGTGGCAAAGCT | 59.8 | 0.00 |
| mm4_exon_Hyls1/Pate2_chr9_35570371_F | TCGTCGGCAGCGTCTGTTAGTCTGGCGGTTAGGC | 59.7 | 0.00 |
| mm4_exon_Hyls1/Pate2_chr9_35570371_R | GTCTCGTGGGCTCGGAGTGGAGACCGACTCACGAG | 60.9 | 0.00 |
| mm4_exon_Gm17182_chr5_53049010_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_exon_Gm17182_chr5_53049010_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gpatch2|Esrrg_chr1_187547551_F | TCGTCGGCAGCGTCCCAGTAGTCTGCCAACAGGG | 60.0 | 0.00 |
| mm4_intergenic_Gpatch2|Esrrg_chr1_187547551_R | GTCTCGTGGGCTCGGTGGGGCTTTGCTTTAGGGAG | 59.9 | 0.00 |
| mm4_exon_Prosc_chr8_27055869_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_exon_Prosc_chr8_27055869_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Ldlrad3_chr2_102123879_F | TCGTCGGCAGCGTCCAAAGCTTTGGCCACTGTGG | 60.2 | 0.00 |
| mm4_intron_Ldlrad3_chr2_102123879_R | GTCTCGTGGGCTCGGAGTAGAAAGGGCAGCAGGTG | 59.6 | 0.00 |
| mm4_intergenic_Gm5627|Ryk_chr9_102773858_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm5627|Ryk_chr9_102773858_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_exon_Slc15a5_chr6_138055781_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_exon_Slc15a5_chr6_138055781_R | Primer3: not found at this Tm | N.d. | 0.00 |
These only list off-targets that have primers in the table above. Primers underlined, off-targets in bold.
| ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52313632 |
TTGCAGCAGGAGGATGACAGCTTAGAAAGAAGAGGGCAATGGGGCTTCCTCCCAGAGGCGGTGCGGCACAGAGGAGCGCT CGCATCACAAGGTGACCCTAGCTCCCCACTGCCATCT |
| mm4_exon_Pcdhb9_chr18_37401631 |
ATACACAGTCAGCCCCAACCCTTATTTTCACGTCCTCACTCAAAATAATAGTAAAGGCAAAAAATACCCAGAGTTGGTGC AGGACAGAGGCCTGGACAGAGAGGAGCAGGCAGAGCTGAGCTTAACC |
| mm4_exon_Pcdhb7_chr18_37342477 |
CACAGTCAGCCCCAACTCTCATTTTCATGTCCTCACTCGAAATAATAGCGAGGGCAAGAAATACCCAGAGTTGGTGCAGG ACAGAGCCCTGGACAGAGAGGAGCAGGCAGAGCTGAGCTTAACC |
| mm4_intron_Blk_chr14_63413701 |
GAAGCAGAGCACAAACAGGCCACTGGCTCACTTTTTCCCAGCTGTCAGGTTCTAAGCCATGTTCCCGTTTAGACCTCTGT ACCACACCACCTCAAGGGAAGTAGAATGTCAAAAATTCGCAGTGCCACTTCC |
| mm4_exon_Gpr26_chr7_131966615 |
AAAGCCAGTGACCACAGGAGACTGCGACCCCGGATTCCAGGGCACTTCAGGAGGTCAGAGGAGCACAGGCCAAGAGGTGG TGCGGGACTGGGAGTGAACACTCGCCTAGGTGATCG |
| mm4_exon_Dcun1d4_chr5_73491429 |
GGGGACATTCTGGAAGGCCGGGGGCCGAGAGTCGGTGGTGCACGGAGGTGGAGACGGCGGCGGGCGCTGGCAGCTGGGGT CGGGGGTGGTACGAGCGGGCAGGGAGAGGGCCTGCGGTCCGAGTACAGGGTGAGGCTTG |
| mm4_intergenic_Gm24467|Gm13456_chr2_40334846 |
TGCTCTCTCTGTCAGTTTTCTTTCTCTCTGCCTCACTGCTTCTAGGTAATCTTAGTGAGTTCAACCAATTTTTTATGAAA ATGTAAGCAACGTTACTGACATATAGTATAGTTGAAAATATTGCTGCCCATAAATCCCCT |
| mm4_intergenic_Tsen54|Llgl2_chr11_115823767 |
TCTCTCGCTTGGCTCTTGTGCTTTCAAGTCCGCTGCCTGCATGGAGTCGCGTCCATCCTGGAAAGCACCCAGGGACTCTT TCCTCTTCCCAGAGGCGGTGGGACACCTAGAGCTGTGAGCAGTCCTGTGACCAG |
| mm4_intron_Dpp10_chr1_123632814 |
TCATACCCAGCACTCAGGGAGCCCACCATGTTCCTCTCTGCTGCACCCACTCTGGGGCCTTTCATGACTGTATTCTATAT GTGCTTAAAAGCATTATCTCCCATGTTTGTTTATTTGCTCA |
| mm4_intergenic_mmu-mir-7052/Josd2|Josd2_chr7_44470549 |
GTCAACGTGATCATGGCTGCCCTGCAGGGCCTGGGCCTGGCCGCTGTGTGGTGGGACAGGAGGAGGTAGGGCCTAGGCTG TGGGCTGCACTGCCTCTGGGCGTGTGGGATGACACAGAGGG |
| mm3_exon_Hk3_chr13_55012247 |
ACCAGTTCAGCATCTGAGGCCCGAGGGCTCAGACCCAAGTCCTGCAGGATGGTGTGGACGCGGGCTGTCCCAGTGGCGGT GCTGCAAAGAGGAGGGTGAAGACACATGGGTGGT |
| mm4_intron_Ctbp2_chr7_133037473 |
TAGAGGGCCAACAAAACCCCTTTTAATTGTCCTCTGACCAGCTCCGCCTCTGGGGTCCCCTGTCTGGGATCCTGACAGGC AAATAGTCTTTGTTCACCCACAGAGGGTCAATGGGTC |
| mm4_intergenic_Tpd52|Zbtb10_chr3_9098781 |
CTGCTCACAAAATGCGTGCTCCGCCCCAGGCAGGACGGTCCGTCCTCCGAGCTGCTCCGCCTCTGGGGAACCTGGTTGGA AAAGACCAGTGAATAGACTTAAGGCTTTTGTTGTCGCTGTTGC |
| mm4_exon_Mettl24_chr10_40683409 |
CACTGTCAAGCCTCCCGGGCGTGGTTGTGGAGCCCTGCCCCGGTGGCTGCTCGGTGCCGCGCTGCTTCTGGGCCTACGGC TTTGCATGGAGTTG |
| mm4_intergenic_n-R5s21|Cpn1_chr19_43939854 |
GGGGCTCAGTTCTTCAACTCAAAGCCAAGAGGCAGGGCGGCGCAGTGACAGCGCCCCGGCTGGACACCTCGCCCCCCGGT CCGCGCCCCGGGACGGTCCCTAGGGCTG |
| mm4_intron_2900026A02Rik_chr5_113104447 |
GTCTTGGTACCCTGGCTCTGCCGTAGTTCTGCTAAGTCTTAAATCAGGGGAACCAAATCCTAGACAGCAGACCCAGGGGT GGTGGGGCTCAGAGGCCATTCAGTCTGCACTTTGCCTGCAGAGGAGGAA |
| mm4_intergenic_Npy2r|Gm22038_chr3_82787231 |
GCCCATGCCTCTGTGTTACTGACAGTAACACCTTCTGCCCAGACCAAGGGGCTGTGCTGCACAGGGATCGTGGTTTTTCC CTCCTAGTTAAATCTTTTGTAGTACATTTTAAGGGCTTACTGGA |
| mm4_exon_Mast3_chr8_70783782 |
CATTCGTCTCGTCGTCCTCGGAGCCCAAGTGGCGGTACCGCTCAGAGCGAGCTGTGGGAGGGAAGGGGAGGCTGGGGCAG CCAGGGACACCCAGAGGTCCAGGGCTGTCCTGCTCAGTTACAGGAGAATGGCCCAGC |
| mm4_intron_Tcof1_chr18_60824477 |
GCCTGCAGTGCTTTACCAAGCTCCCACCGCCCCAGGGGCTGTGAGACACAGAGAAAGCAACAAACTTTAGCACTATGGTA CCCACCAAGTGTGGCGGCGGCACCGCTGCGCTCAGGCCCTCCCTACCCTGCCAAACAG |
| mm4_exon_Gpr173_chrX_152346508 |
CAGGAAGCAGACGATTGGGTTGACGGCAGCCTGGGCGAAGCTCATCCAAACAGCAGTGGCCAAGTAGCGGTGGGGCACAG CGCAGGCTTTCACAAACACTCGCCAGTAGCA |
| mm4_intron_Dnm1_chr2_32343696 |
GGAGGACCCCTAAACTTCAGCAAGAAATCTGAAACCCCACAGAAATCTCTGCCTCACCTCCTCTGGGTACTGAATCCTGA CTGAGGCACCATGAA |
| mm4_intergenic_Gm21893|Tead4_chr6_128295978 |
ATGAAGGAGAAGCCCAGTGCTCCATTTCCAGGATGCTCATCTTGTCCCTTGGAGAAAGAAATCAAGATAGCATGTGTTTT CTCTGCCCCACCCCCTCTTGGGTCCTGGCAGTGGTCCTATG |
| mm4_exon_Pcdhb8_chr18_37355842 |
CACAGTCAGTCCCAACCTCCATTTCCATGTCGTTACTCTCAGTCGCTCAGATGACAGGAAATACCCAGAGCTGGTGCTGG ACAGAGCCCTGGACAGGGAGGAGCAGCCTGAACTCACTT |
| mm4_exon_Pcdhb6_chr18_37334599 |
CACAGTCAGTCCCAACCTCCATTTCCATGTGGTTACTCTCAGTCGCTCAGATGGCAGGAAATACCCAGAGCTGGTGCTGG ACAGAGCCCTGGACAGGGAGGAGCAGCCTGAACTCACTT |
| mm4_exon_Pcdhb4_chr18_37308210 |
CTCTCAGTCGCTCAGATGGCAGGAAATACCCAGAGCTGGTGCTGGACAGAGCCCTGGACAGGGAGGAGCAGCCTGAGCTC ACTTTAATCCTCACCGCTCTGGATGGGGGAGCTCCACGAAGGTCTGGGATGAC |
| mm4_exon_Pcdhb12_chr18_37436353 |
CTCTCAGTCGCTCAGATGGCAGGAAATACCCAGAGCTGGTGCTGGACAGAGCCCTGGACAGGGAGGAGCAGCCTGAGCTC ACTTTAACCCTCACTGCTCTTGATGGCGGATC |
| mm4_exon_Pcdhb11_chr18_37422190 |
GTCGCTCAGATGGCAGGAAATACCCAGAGCTGGTGCTGGACAGAGCCCTGGACAGGGAGGAGCAGCCTGAGCTCACTTTA ATCCTCACCGCTCTGGATG |
| mm4_exon_Pcdhb10_chr18_37412444 |
CACAGTCAGTCCCAACCTCCATTTCCATGTGGTTACTCTCAGTCGCTCAGATGGCAGGAAATACCCAGAGCTGGTGCTGG ACAGAGCCCTGGACAGGGAGGAGCAGCCTGAACTCACTT |
| mm4_intergenic_Gm3833|Fhit_chr14_10278289 |
TTGGTTGCTTGCTGGCATTCACTGCTGAACATGTCAGCCTAACTCAAAAGGCGTGGCTTTCTCTCTGTCTTCCTCCCTGC CGCACCTACTCTTGGCAGCTATTATGATTTGCAGCTTTTCCCC |
| mm4_intergenic_1810011O10Rik/Gm26714|Ido2_chr8_24518288 |
CAATTCCTCCGCCATCACCTCAGCAAGGTACTCTGTGACCCCCCACCTCTGGGTTCATGAGCAGAACTGGCTATGAATGT CCCTTGTGGTCTCTCCTTTCCTTTTCCATCTTTGAGGAACCCTGCAGGTGATCTCACAG |
| mm4_intergenic_Gm14947|Gm14940_chrX_122606496 |
GGCTCATACCTAGACCCATTGGTCTGCCACTCTGTCCCTCCCAGCCTCTTGGCACCTTTGTGGGCCCCAGGCCACTCATA CTATCCCTAAGTCCCTGGACCTACCACTAGGCTCCTCTGCCTACCTGTTGGCTCCTTGG |
| mm4_intron_Nsmce4a_chr7_130560758 |
CAGACTCCAGCAAGAGAGACAGAGAAAGCTCCAGGAGGCAGTGAGACACAGAGAAGGAAGCAGAAACATCCTAAAAAGGG TGAAATAAAGGATTTCCAAGAGCAAGGTGGGG |
| mm4_exon_Hddc2_chr10_31313506 |
TAGTTGGGCAGCTCAAGGTGAGCGGGTGGGATCGGCTTGGCCGGGAGGCGGTGCGGCCCAGCTCAGGCACCGACCTCACG CAACCTCCC |
| mm4_intergenic_A530013C23Rik|Gm14321_chr2_167769918 |
GGAGACACAGCCTGGAGAAGGGCTGGCAACCCCACGGCCCAGAGGCTGGGAGGCCCAGAGGCGGGGAGGCCCAGTCGGAA CTTCTGGTTTCCGTGTTGTCCAGTTGGTGAGGTTCCTGTTCGG |
| mm4_intron_Nsf_chr11_103829528 |
GGCAAGCTCCTGAGTGACTTCGGAATGACCTTGACCAGAGAAGTAAGAGAATAAAGGCGACAACAGCAAACCCAAAGACT GTGCTGCACAGAGAGGAGGCAGCCTACACCCAGGGGAGGGGTCTGCCAGAGGAAAAT |
| mm4_intergenic_Gm5432|Lpin1_chr12_16491463 |
CCTTGGGCGATGTTCTCAGAAAGAAGCTGTGATGCAGTTAAGTGGAGCCAGCTTCACCTCTGTGCCCCTCTGCCCCTGGG GGTCAGAATTCAAGGTGTGTGCCA |
| mm4_intergenic_Ptchd1|Gm15155_chrX_155624149 |
GGAAAGACGCAGGCTGAAGACTGCGTGCGCCCCGGCTCTTGCCAGGCTCAGGTGGTGGCGCGCACTGGCTATTACCGAGA GGGGGTGGGGAACCGAGACGCTCGAGCATGGAAATCACGCCTCCTCCCAAG |
| mm4_intron_Gse1_chr8_120296593 |
CATTTGGGCTTTGGGCTGTCAGGTCCGGAAGTGTCCCGCTCTGTGTCCCGCCTCCTCTTGGCCCCAGTGGTCTGTACATC TCTGTCCACTTGGTAGCGATGGGTT |
| mm4_exon_Angptl4_chr17_33778434 |
CAGAGGCTGGATCTGGAAAAGTCCACTGTGCCGCTCCCCTTCTTGGAAGAGTTCCTGGCAGTCCCGGGGCGGCCCTGAAA GTAGACAA |
| mm4_intron_Lmx1b_chr2_33601332 |
TGAAAGCCTGTCTGCCTCTGCAGCCTCCCACCTACCCTTGTGGGGAGGAGCATCCTAAAGAGGGTGCGGCATAGAGGAAG TGCACCGTAAAGCAGGA |
| mm3_intergenic_Tbc1d22a|Gm23416_chr15_86554626 |
CAGATCTCAGACTTGGCAGGTCTAAATGGAAGAGGTGACTTTGGTAGACCTTGAAGGATTCTACCAGCTGGTCCTGGAGG CGGAGAGGCACAGAGAGAGGGAGGAATGCATTTCAGAGTCTCAGTGGGAGGAAGGC |
| mm4_intergenic_Lrpap1|Adra2c_chr5_35213675 |
AGAGACGTAGAGGGGTGCATGCTCCCCAACACGCTCCTCTCCTTCTGAAGCACCAGGCTCTGTTCCACACCTTCTCTAGG TGGCATCATGTCTTGCGCCTCACCTCCTGCAGGAAGCTTGGGAGAGCCTCAG |
| mm4_intron_Gm20388/Galnt2_chr8_124332178 |
AAAGTAAGTGCGGCCTTTGCAGGAAGCCCTCCTAGGAGAGCCCAGACTCCAGCCCCTGTTGCAGTATGCATCCAGGACAG TGAGGCTCTGTGCCTCCCACCCTCTGGGTTCCTGGCCTCCCTTCAAGGCCAACAT |
| mm4_intron_Fam65c_chr2_167981235 |
CAGCTGAGGTAGTCTTCCCCCATCTCCACTAGAACTCTAGGACCCCACTGTGCCGCACAGGCCCTTGGGCTGCACTTTAC CAAATGACAGTGTCGCTTCCCGCGCTGCAACTCGCACGGCCTCCAGGTCAGACTGACAC |
| mm4_intergenic_Actrt2|Gm13113_chr4_154695068 |
CACAGTAGAAGGGACCAGCGTATGAGACACCCTTCACCCATCCCACAGGCTGTGCCCCACAGAGGGAAGCTTCAGCCGGG CTGCTAAGCTGCCAAAGGCCTTCACAACTGC |
| mm4_intergenic_A930031H19Rik|Gm25261_chr4_131161047 |
GTGGCATTCTGTCCTCTGCTCTCTGCTGGGACGTGGCAGGCTTCACAGATGACATCGGGATGTCAGAGCCCTGGGCTCTC CGTGCCCCACTGCCTCCGGGCAGTTCACAGAAGGCCCCTCACTCCAGAAACCCAC |
| mm4_intergenic_Gm17388|Htr1d_chr4_136408017 |
TACTCCGTGAAGCTGCCAAGAAGCAGAGAGCGTGGCCCTCCAGTGACCCCTCCCTGTGCCGCAGCAGCTCTTGGTGGTCC TTTGAATACAAGACTTCTTTTCCCGAGTGTACACTGGAAAGCATCGCACAGTTGG |
| mm4_intron_Dscam_chr16_96934933 |
GGCTCTGTCACACACCATCATTCAGGAGAAGCCAGCCCTTCTTCCCTCCCTTTGTGCCCCACCTTCTCTTGGCATGCACA ATGTCTCCAGATCTATGCTTCTACCACACACCTCCC |
| mm4_intron_Gm26510_chr17_70892388 |
AGTGCTCCTGATTGTGCCTGGTGCACAGCTCTGTGCCGGGCAGCCTCCTGGCCCTCAATCACTGTGTATGGCTTCCTATG AAAACTTCAAAGCAATCCGTTCCTTTGACTCTGGTACCCAGTTGACTGAGCGCTTTT |
| mm4_intergenic_Med27|Gm24976_chr2_29434325 |
CCTGCTGAGGATGAAGCTCCTGAACTGAAGCAGCCTTACAAAGAGCTGGGTCCTGCCTGTCAGTGCGTCTCAGTGCCGCA CCTGCTTTGGGGACACAACATACCTGAAGGGTCTCCTGACTGTGGT |
| mm4_intergenic_Slc25a36|Rpl7a-ps10_chr9_97146518 |
AGTCAATGTGAGGTCCTGCTTACTCATCCCCACAGGAGGTCCAGCACAGAGCCTTGTCTTGAGAAAAGCCCCTTGCTGAC TTGCCTTGGAGGTGGGTGAGATGTTACAGCAAGCTTTGCCACCTCTTGTCA |
| mm4_exon_Hyls1/Pate2_chr9_35570371 |
TGTTAGTCTGGCGGTTAGGCAACGGGCTGGCCCTGCTCCGTGCCGCAGCCGCTCTTGGCTGTGGCCGGCGGCGTCGGAGG GTAGCGGAGGGGAGGGTTCTGGGGCGTGGCTGGCCCGGCTCGTGAGTCGGTCTCCACT |
| mm4_intergenic_Gpatch2|Esrrg_chr1_187547551 |
CCAGTAGTCTGCCAACAGGGAGGATGTTTGATCATGTGACCCTCAATAGAAATGCCTACAGGAGGAGGGGCACAGAGGCT TCTCCACATCCAACACCCCCTCCCTAAAGCAAAGCCCCA |
| mm4_intron_Ldlrad3_chr2_102123879 |
CAAAGCTTTGGCCACTGTGGAGCACAGAAGAGGTGGCCCAGGAGGAGGGGCGGCAAAGAGAAGCCAGCCTTCACCCCTTG CCCACCTGCTGCCCTTTCTACT |