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Contents:

PCR primers for off-targets of CCCTCTGGAGAGTCCGGGGA TGG

In the table below, Illumina Nextera Handle sequences have been added and highlighted in bold. Primers for the on-target have been added for convenience. The table below is sorted by the CFD off-target score. Sites with very low CFD scores < 0.02 are unlikely to be cleaved, see our study Haeussler et al. 2016, Figure 2.

In the protocol by Matthew Canver, Harvard, two PCRs are run: one PCR to amplify the potential off-target, then a second PCR to extend the handles with Illumina barcodes. Please click here to download the protocol. Alternatively, you can have a look at Fu et al, 2014.

If a primer was not found, the reason is usually that the region around the off-target is too repetitive. To avoid unspecific primers, all repeats are masked for the primer design (not for off-target search). If you think that we should change the parameters here or should use different primer3 settings, please let us know.

Maximum amplicon length:     Primer Tm:

NamePrimer Sequence Tm CFD Score
ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52313825_F TCGTCGGCAGCGTCAGGGAGCCTGGGATTAGGAG 60.1 1.00
ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52313825_R GTCTCGTGGGCTCGGTACCAACCAGAGTGCCAAGC 60.2 1.00
mm2_intergenic_4930552P12Rik|Tcf7l2_chr19_55733090_F Primer3: not found at this Tm N.d. 0.71
mm2_intergenic_4930552P12Rik|Tcf7l2_chr19_55733090_R Primer3: not found at this Tm N.d. 0.71
mm4_intron_Erp27_chr6_136915945_F Primer3: not found at this Tm N.d. 0.61
mm4_intron_Erp27_chr6_136915945_R Primer3: not found at this Tm N.d. 0.61
mm2_intergenic_Rxrb|Col11a2_chr17_34039129_F TCGTCGGCAGCGTCGATTACGAGTGGGCGTGGAA 60.1 0.57
mm2_intergenic_Rxrb|Col11a2_chr17_34039129_R GTCTCGTGGGCTCGGTAGTCCTTTCACACCACGGC 59.9 0.57
mm4_intron_Zfp64_chr2_168911034_F Primer3: not found at this Tm N.d. 0.54
mm4_intron_Zfp64_chr2_168911034_R Primer3: not found at this Tm N.d. 0.54
mm3_intron_Btbd16_chr7_130797858_F TCGTCGGCAGCGTCTGCTTCTGGCTTTGACCCTT 59.8 0.50
mm3_intron_Btbd16_chr7_130797858_R GTCTCGTGGGCTCGGAGGAGGACACATGCACAGTG 59.9 0.50
mm4_intergenic_Gm24035|Cdh13_chr8_118459731_F Primer3: not found at this Tm N.d. 0.44
mm4_intergenic_Gm24035|Cdh13_chr8_118459731_R Primer3: not found at this Tm N.d. 0.44
mm4_intron_Kctd3_chr1_188988363_F TCGTCGGCAGCGTCATGGGGCTGGGTTTTCCAAA 60.1 0.39
mm4_intron_Kctd3_chr1_188988363_R GTCTCGTGGGCTCGGACCTGAGAGGAGCCAGAAGT 59.8 0.39
mm4_intergenic_Kcnf1|Pdia6_chr12_17179267_F Primer3: not found at this Tm N.d. 0.39
mm4_intergenic_Kcnf1|Pdia6_chr12_17179267_R Primer3: not found at this Tm N.d. 0.39
mm4_intergenic_Zfp335|Gm11458/Zfp335_chr2_164911234_F TCGTCGGCAGCGTCCGGGGTCAGTGGTTCACTC 60.0 0.36
mm4_intergenic_Zfp335|Gm11458/Zfp335_chr2_164911234_R GTCTCGTGGGCTCGGGACAGCCCCGAGACACCAG 62.3 0.36
mm4_intron_Spred2_chr11_20006528_F Primer3: not found at this Tm N.d. 0.32
mm4_intron_Spred2_chr11_20006528_R Primer3: not found at this Tm N.d. 0.32
mm4_exon_Lhx3_chr2_26200605_F Primer3: not found at this Tm N.d. 0.31
mm4_exon_Lhx3_chr2_26200605_R Primer3: not found at this Tm N.d. 0.31
mm4_intron_Dph5_chr3_115899115_F Primer3: not found at this Tm N.d. 0.31
mm4_intron_Dph5_chr3_115899115_R Primer3: not found at this Tm N.d. 0.31
mm4_intergenic_Mvb12b|Gm13403_chr2_33898619_F Primer3: not found at this Tm N.d. 0.28
mm4_intergenic_Mvb12b|Gm13403_chr2_33898619_R Primer3: not found at this Tm N.d. 0.28
mm3_intergenic_1700036O09Rik|Gm14689_chrX_67473959_F Primer3: not found at this Tm N.d. 0.25
mm3_intergenic_1700036O09Rik|Gm14689_chrX_67473959_R Primer3: not found at this Tm N.d. 0.25
mm4_intergenic_Gm16080|Ptpru_chr4_131721933_F Primer3: not found at this Tm N.d. 0.24
mm4_intergenic_Gm16080|Ptpru_chr4_131721933_R Primer3: not found at this Tm N.d. 0.24
mm4_intergenic_Cers3|Adamts17_chr7_66835373_F TCGTCGGCAGCGTCGACCATGGAATCAAGCCGGA 60.1 0.23
mm4_intergenic_Cers3|Adamts17_chr7_66835373_R GTCTCGTGGGCTCGGTGGGGCTATCTGAGGGTGAA 59.9 0.23
mm4_intergenic_mmu-mir-7239|Cyfip2_chr11_46181851_F Primer3: not found at this Tm N.d. 0.23
mm4_intergenic_mmu-mir-7239|Cyfip2_chr11_46181851_R Primer3: not found at this Tm N.d. 0.23
mm4_intron_Col9a2_chr4_121040107_F TCGTCGGCAGCGTCGCTATTCAGTCGACAGCGGA 59.8 0.22
mm4_intron_Col9a2_chr4_121040107_R GTCTCGTGGGCTCGGCATATTGGGGCGACAGGGAA 59.8 0.22
mm4_exon_Susd4_chr1_182764167_F Primer3: not found at this Tm N.d. 0.21
mm4_exon_Susd4_chr1_182764167_R Primer3: not found at this Tm N.d. 0.21
mm4_intergenic_Gm22507|Trhde_chr10_114071766_F Primer3: not found at this Tm N.d. 0.21
mm4_intergenic_Gm22507|Trhde_chr10_114071766_R Primer3: not found at this Tm N.d. 0.21
mm4_intron_Antxr1_chr6_87158190_F Primer3: not found at this Tm N.d. 0.21
mm4_intron_Antxr1_chr6_87158190_R Primer3: not found at this Tm N.d. 0.21
mm4_intergenic_Gm17055|Bhlhe40/0610040F04Rik_chr6_108659708_F Primer3: not found at this Tm N.d. 0.20
mm4_intergenic_Gm17055|Bhlhe40/0610040F04Rik_chr6_108659708_R Primer3: not found at this Tm N.d. 0.20
mm4_intron_Sned1_chr1_93241390_F Primer3: not found at this Tm N.d. 0.19
mm4_intron_Sned1_chr1_93241390_R Primer3: not found at this Tm N.d. 0.19
mm4_intron_Uty_chrY_1203306_F Primer3: not found at this Tm N.d. 0.19
mm4_intron_Uty_chrY_1203306_R Primer3: not found at this Tm N.d. 0.19
mm4_intron_Gm13849_chr6_31914158_F Primer3: not found at this Tm N.d. 0.19
mm4_intron_Gm13849_chr6_31914158_R Primer3: not found at this Tm N.d. 0.19
mm4_intron_Dpp10_chr1_123870418_F Primer3: not found at this Tm N.d. 0.19
mm4_intron_Dpp10_chr1_123870418_R Primer3: not found at this Tm N.d. 0.19
mm4_intergenic_Stac|Arpp21_chr9_111849361_F Primer3: not found at this Tm N.d. 0.19
mm4_intergenic_Stac|Arpp21_chr9_111849361_R Primer3: not found at this Tm N.d. 0.19
mm4_intergenic_St8sia2|AC114591.1_chr7_74084092_F Primer3: not found at this Tm N.d. 0.19
mm4_intergenic_St8sia2|AC114591.1_chr7_74084092_R Primer3: not found at this Tm N.d. 0.19
mm4_intergenic_Gm26475|Gm24522_chrX_128619008_F Primer3: not found at this Tm N.d. 0.19
mm4_intergenic_Gm26475|Gm24522_chrX_128619008_R Primer3: not found at this Tm N.d. 0.19
mm4_intergenic_Gm25977|Gm11875_chr4_21428592_F Primer3: not found at this Tm N.d. 0.19
mm4_intergenic_Gm25977|Gm11875_chr4_21428592_R Primer3: not found at this Tm N.d. 0.19
mm4_intergenic_Gm24442|Col1a2_chr6_4476193_F Primer3: not found at this Tm N.d. 0.19
mm4_intergenic_Gm24442|Col1a2_chr6_4476193_R Primer3: not found at this Tm N.d. 0.19
mm4_intergenic_Gm23752|Gm24815_chr12_7276176_F Primer3: not found at this Tm N.d. 0.19
mm4_intergenic_Gm23752|Gm24815_chr12_7276176_R Primer3: not found at this Tm N.d. 0.19
mm4_intergenic_Eif3s6-ps2|Gm25418_chr18_90498591_F Primer3: not found at this Tm N.d. 0.19
mm4_intergenic_Eif3s6-ps2|Gm25418_chr18_90498591_R Primer3: not found at this Tm N.d. 0.19
mm4_intergenic_Edem1|Gm20387_chr6_109061566_F Primer3: not found at this Tm N.d. 0.19
mm4_intergenic_Edem1|Gm20387_chr6_109061566_R Primer3: not found at this Tm N.d. 0.19
mm4_intergenic_Cntn5|Gm25365_chr9_11019535_F Primer3: not found at this Tm N.d. 0.19
mm4_intergenic_Cntn5|Gm25365_chr9_11019535_R Primer3: not found at this Tm N.d. 0.19
mm4_intron_Tldc1_chr8_119765814_F Primer3: not found at this Tm N.d. 0.19
mm4_intron_Tldc1_chr8_119765814_R Primer3: not found at this Tm N.d. 0.19
mm4_intergenic_Gm22777|Spock1_chr13_57118638_F Primer3: not found at this Tm N.d. 0.18
mm4_intergenic_Gm22777|Spock1_chr13_57118638_R Primer3: not found at this Tm N.d. 0.18
mm4_intron_Rims2_chr15_39522657_F Primer3: not found at this Tm N.d. 0.18
mm4_intron_Rims2_chr15_39522657_R Primer3: not found at this Tm N.d. 0.18
mm4_intron_Aff1_chr5_103778575_F Primer3: not found at this Tm N.d. 0.17
mm4_intron_Aff1_chr5_103778575_R Primer3: not found at this Tm N.d. 0.17
mm4_intron_Fam163b_chr2_27118669_F Primer3: not found at this Tm N.d. 0.17
mm4_intron_Fam163b_chr2_27118669_R Primer3: not found at this Tm N.d. 0.17
mm4_intron_Lrrc4c_chr2_97429036_F Primer3: not found at this Tm N.d. 0.16
mm4_intron_Lrrc4c_chr2_97429036_R Primer3: not found at this Tm N.d. 0.16
mm3_intergenic_Rab17|Lrrfip1_chr1_90972880_F Primer3: not found at this Tm N.d. 0.16
mm3_intergenic_Rab17|Lrrfip1_chr1_90972880_R Primer3: not found at this Tm N.d. 0.16
mm4_intergenic_Gm5868|Cnga1_chr5_72602552_F Primer3: not found at this Tm N.d. 0.15
mm4_intergenic_Gm5868|Cnga1_chr5_72602552_R Primer3: not found at this Tm N.d. 0.15
mm4_intergenic_Gm22312|Magi1_chr6_93562550_F Primer3: not found at this Tm N.d. 0.15
mm4_intergenic_Gm22312|Magi1_chr6_93562550_R Primer3: not found at this Tm N.d. 0.15
mm4_intergenic_1700012P22Rik|Gm9944_chr4_144444010_F Primer3: not found at this Tm N.d. 0.15
mm4_intergenic_1700012P22Rik|Gm9944_chr4_144444010_R Primer3: not found at this Tm N.d. 0.15
mm4_intergenic_Stk32c|U6_chr7_139191650_F Primer3: not found at this Tm N.d. 0.15
mm4_intergenic_Stk32c|U6_chr7_139191650_R Primer3: not found at this Tm N.d. 0.15
mm4_intergenic_Rhobtb2|Pebp4_chr14_69837091_F Primer3: not found at this Tm N.d. 0.14
mm4_intergenic_Rhobtb2|Pebp4_chr14_69837091_R Primer3: not found at this Tm N.d. 0.14
mm3_intergenic_Gm14246|Gm22936_chr2_162742448_F TCGTCGGCAGCGTCGTGACCACTTACAGGCAGCT 59.9 0.14
mm3_intergenic_Gm14246|Gm22936_chr2_162742448_R GTCTCGTGGGCTCGGTCGTGAGAACTGTCTGGAGC 59.4 0.14
mm4_intergenic_CT030233.1|Gm20675_chr14_67157566_F Primer3: not found at this Tm N.d. 0.14
mm4_intergenic_CT030233.1|Gm20675_chr14_67157566_R Primer3: not found at this Tm N.d. 0.14
mm4_exon_Sec62/4933429H19Rik_chr3_30793550_F Primer3: not found at this Tm N.d. 0.13
mm4_exon_Sec62/4933429H19Rik_chr3_30793550_R Primer3: not found at this Tm N.d. 0.13
mm4_intergenic_Gm11981|Gm11977_chr11_6761502_F Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm11981|Gm11977_chr11_6761502_R Primer3: not found at this Tm N.d. 0.12
mm4_intron_Gm13387_chr2_25130289_F Primer3: not found at this Tm N.d. 0.12
mm4_intron_Gm13387_chr2_25130289_R Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Xpnpep1|Add3_chr19_53087719_F Primer3: not found at this Tm N.d. 0.11
mm4_intergenic_Xpnpep1|Add3_chr19_53087719_R Primer3: not found at this Tm N.d. 0.11
mm4_intergenic_Cenpw|Gm22623_chr10_30436091_F Primer3: not found at this Tm N.d. 0.11
mm4_intergenic_Cenpw|Gm22623_chr10_30436091_R Primer3: not found at this Tm N.d. 0.11
mm4_intron_Ablim2_chr5_35794655_F TCGTCGGCAGCGTCGCATAGGCCTTAGGGTTGCA 60.1 0.11
mm4_intron_Ablim2_chr5_35794655_R GTCTCGTGGGCTCGGGTAACCTGAGGCCAAGAGCA 59.6 0.11
mm4_intron_Unc13c_chr9_73952449_F TCGTCGGCAGCGTCGGCATGACTGAGTTGGGTCA 59.9 0.10
mm4_intron_Unc13c_chr9_73952449_R GTCTCGTGGGCTCGGCACCCAGAGTAGGAAGCAGC 60.1 0.10
mm4_intergenic_Frem2|Gm24851_chr3_53744727_F Primer3: not found at this Tm N.d. 0.10
mm4_intergenic_Frem2|Gm24851_chr3_53744727_R Primer3: not found at this Tm N.d. 0.10
mm4_exon_Tnfaip2_chr12_111445096_F Primer3: not found at this Tm N.d. 0.10
mm4_exon_Tnfaip2_chr12_111445096_R Primer3: not found at this Tm N.d. 0.10
mm4_exon_Shisa9_chr16_11984727_F Primer3: not found at this Tm N.d. 0.10
mm4_exon_Shisa9_chr16_11984727_R Primer3: not found at this Tm N.d. 0.10
mm4_intergenic_Tle3|Rplp1_chr9_61630302_F Primer3: not found at this Tm N.d. 0.09
mm4_intergenic_Tle3|Rplp1_chr9_61630302_R Primer3: not found at this Tm N.d. 0.09
mm4_intron_Adcy7_chr8_88313430_F TCGTCGGCAGCGTCGACTGCTGACTCCCATCCAG 59.8 0.09
mm4_intron_Adcy7_chr8_88313430_R GTCTCGTGGGCTCGGGGAAGGAAGAGGGAAGGTGC 60.0 0.09
mm4_intergenic_Gm5973|Gm9915_chr1_40920481_F Primer3: not found at this Tm N.d. 0.09
mm4_intergenic_Gm5973|Gm9915_chr1_40920481_R Primer3: not found at this Tm N.d. 0.09
mm4_intergenic_Emc2|Tmem74_chr15_43602626_F Primer3: not found at this Tm N.d. 0.09
mm4_intergenic_Emc2|Tmem74_chr15_43602626_R Primer3: not found at this Tm N.d. 0.09
mm4_intron_Mmp24_chr2_155805696_F Primer3: not found at this Tm N.d. 0.09
mm4_intron_Mmp24_chr2_155805696_R Primer3: not found at this Tm N.d. 0.09
mm4_intergenic_Hecw1|Mrpl32_chr13_14563161_F Primer3: not found at this Tm N.d. 0.08
mm4_intergenic_Hecw1|Mrpl32_chr13_14563161_R Primer3: not found at this Tm N.d. 0.08
mm4_intergenic_Gm7910|Khdrbs2_chr1_31790527_F Primer3: not found at this Tm N.d. 0.08
mm4_intergenic_Gm7910|Khdrbs2_chr1_31790527_R Primer3: not found at this Tm N.d. 0.08
mm4_intergenic_Gm14751|Rps24-ps3_chrX_79123850_F Primer3: not found at this Tm N.d. 0.08
mm4_intergenic_Gm14751|Rps24-ps3_chrX_79123850_R Primer3: not found at this Tm N.d. 0.08
mm4_intron_Map3k10_chr7_27661707_F TCGTCGGCAGCGTCGTACTGCAGGGTGGTAAGCA 59.6 0.08
mm4_intron_Map3k10_chr7_27661707_R GTCTCGTGGGCTCGGTGAAGCACTGACCGCTACTG 60.0 0.08
mm4_intergenic_Fbxo21|Tesc_chr5_118010562_F TCGTCGGCAGCGTCACACCGCTAGGCTACTCAGA 60.0 0.08
mm4_intergenic_Fbxo21|Tesc_chr5_118010562_R GTCTCGTGGGCTCGGGGCTTGACTGAGGAAGACCC 60.0 0.08
mm4_intron_Klf8_chrX_153293911_F Primer3: not found at this Tm N.d. 0.08
mm4_intron_Klf8_chrX_153293911_R Primer3: not found at this Tm N.d. 0.08
mm4_intergenic_Gm25335|Magi2_chr5_19566729_F Primer3: not found at this Tm N.d. 0.08
mm4_intergenic_Gm25335|Magi2_chr5_19566729_R Primer3: not found at this Tm N.d. 0.08
mm4_intron_Fgf13_chrX_59433870_F Primer3: not found at this Tm N.d. 0.08
mm4_intron_Fgf13_chrX_59433870_R Primer3: not found at this Tm N.d. 0.08
mm4_intergenic_Gm14561|Gm6071_chrX_22235595_F Primer3: not found at this Tm N.d. 0.08
mm4_intergenic_Gm14561|Gm6071_chrX_22235595_R Primer3: not found at this Tm N.d. 0.08
mm4_intergenic_Kdm6b|Dnah2_chr11_69420123_F Primer3: not found at this Tm N.d. 0.07
mm4_intergenic_Kdm6b|Dnah2_chr11_69420123_R Primer3: not found at this Tm N.d. 0.07
mm4_intergenic_Mir694|Ccbe1_chr18_66230038_F Primer3: not found at this Tm N.d. 0.07
mm4_intergenic_Mir694|Ccbe1_chr18_66230038_R Primer3: not found at this Tm N.d. 0.07
mm4_intergenic_Sik1|Hsf2bp_chr17_31919987_F Primer3: not found at this Tm N.d. 0.07
mm4_intergenic_Sik1|Hsf2bp_chr17_31919987_R Primer3: not found at this Tm N.d. 0.07
mm4_intron_Tmc7_chr7_118582405_F Primer3: not found at this Tm N.d. 0.07
mm4_intron_Tmc7_chr7_118582405_R Primer3: not found at this Tm N.d. 0.07
mm4_intergenic_4930578M01Rik|Gm8973_chr15_98998655_F Primer3: not found at this Tm N.d. 0.07
mm4_intergenic_4930578M01Rik|Gm8973_chr15_98998655_R Primer3: not found at this Tm N.d. 0.07
mm4_intron_Itpr2_chr6_146264448_F Primer3: not found at this Tm N.d. 0.07
mm4_intron_Itpr2_chr6_146264448_R Primer3: not found at this Tm N.d. 0.07
mm4_intergenic_Gm13670|Agps_chr2_75829040_F Primer3: not found at this Tm N.d. 0.06
mm4_intergenic_Gm13670|Agps_chr2_75829040_R Primer3: not found at this Tm N.d. 0.06
mm4_intron_Ezr_chr17_6758120_F Primer3: not found at this Tm N.d. 0.06
mm4_intron_Ezr_chr17_6758120_R Primer3: not found at this Tm N.d. 0.06
mm4_intron_Calml4_chr9_62871012_F Primer3: not found at this Tm N.d. 0.06
mm4_intron_Calml4_chr9_62871012_R Primer3: not found at this Tm N.d. 0.06
mm4_intergenic_Zfp652|Phospho1_chr11_95780681_F TCGTCGGCAGCGTCACACCCAGCAGAAATCTTGGA 59.5 0.06
mm4_intergenic_Zfp652|Phospho1_chr11_95780681_R GTCTCGTGGGCTCGGTGCATAGAACGTCGGACACA 59.4 0.06
mm4_intron_Ltn1_chr16_87414984_F Primer3: not found at this Tm N.d. 0.06
mm4_intron_Ltn1_chr16_87414984_R Primer3: not found at this Tm N.d. 0.06
mm4_exon_Zcchc2_chr1_105990541_F Primer3: not found at this Tm N.d. 0.06
mm4_exon_Zcchc2_chr1_105990541_R Primer3: not found at this Tm N.d. 0.06
mm3_intergenic_Gm24371|BC024582_chr4_32540354_F TCGTCGGCAGCGTCACAGACTGTGTCAGGAACGT 58.8 0.06
mm3_intergenic_Gm24371|BC024582_chr4_32540354_R GTCTCGTGGGCTCGGGGTCCTTGCCCATGTATGCT 60.1 0.06
mm4_intergenic_Ccnt2|Map3k19_chr1_127809806_F Primer3: not found at this Tm N.d. 0.06
mm4_intergenic_Ccnt2|Map3k19_chr1_127809806_R Primer3: not found at this Tm N.d. 0.06
mm4_intergenic_Gm16029/Egflam|Gm16029_chr15_7405104_F Primer3: not found at this Tm N.d. 0.06
mm4_intergenic_Gm16029/Egflam|Gm16029_chr15_7405104_R Primer3: not found at this Tm N.d. 0.06
mm4_intron_Ehf_chr2_103276705_F Primer3: not found at this Tm N.d. 0.05
mm4_intron_Ehf_chr2_103276705_R Primer3: not found at this Tm N.d. 0.05
mm4_intron_Dock1_chr7_134832182_F Primer3: not found at this Tm N.d. 0.05
mm4_intron_Dock1_chr7_134832182_R Primer3: not found at this Tm N.d. 0.05
mm3_intergenic_Gm9843|Usp25_chr16_76470165_F TCGTCGGCAGCGTCCTGTTTTCTGGCTGTGTTGGG 59.9 0.05
mm3_intergenic_Gm9843|Usp25_chr16_76470165_R GTCTCGTGGGCTCGGGTGTCAAGTGCAAGCATGACA 59.6 0.05
mm4_intergenic_Gm24280|Rbm26/Gm17066_chr14_105074324_F Primer3: not found at this Tm N.d. 0.05
mm4_intergenic_Gm24280|Rbm26/Gm17066_chr14_105074324_R Primer3: not found at this Tm N.d. 0.05
mm4_intergenic_Nap1l2|Gm9050_chrX_103193603_F Primer3: not found at this Tm N.d. 0.05
mm4_intergenic_Nap1l2|Gm9050_chrX_103193603_R Primer3: not found at this Tm N.d. 0.05
mm4_intron_Phf17_chr3_41557562_F Primer3: not found at this Tm N.d. 0.05
mm4_intron_Phf17_chr3_41557562_R Primer3: not found at this Tm N.d. 0.05
mm4_intergenic_Gm12122|Slit3_chr11_35478027_F Primer3: not found at this Tm N.d. 0.04
mm4_intergenic_Gm12122|Slit3_chr11_35478027_R Primer3: not found at this Tm N.d. 0.04
mm4_intergenic_Gm23440|Rapgef2_chr3_79041230_F Primer3: not found at this Tm N.d. 0.04
mm4_intergenic_Gm23440|Rapgef2_chr3_79041230_R Primer3: not found at this Tm N.d. 0.04
mm4_intergenic_Gm23960|Gm26373_chr6_21087216_F Primer3: not found at this Tm N.d. 0.04
mm4_intergenic_Gm23960|Gm26373_chr6_21087216_R Primer3: not found at this Tm N.d. 0.04
mm4_intergenic_Gm23083|Gm24773_chr16_80464668_F Primer3: not found at this Tm N.d. 0.04
mm4_intergenic_Gm23083|Gm24773_chr16_80464668_R Primer3: not found at this Tm N.d. 0.04
mm2_intergenic_n-R5s42|Fam19a5_chr15_87215595_F Primer3: not found at this Tm N.d. 0.04
mm2_intergenic_n-R5s42|Fam19a5_chr15_87215595_R Primer3: not found at this Tm N.d. 0.04
mm4_intron_Clnk_chr5_38771605_F Primer3: not found at this Tm N.d. 0.04
mm4_intron_Clnk_chr5_38771605_R Primer3: not found at this Tm N.d. 0.04
mm4_intergenic_Gm4487|Gm24073_chr14_114451568_F Primer3: not found at this Tm N.d. 0.04
mm4_intergenic_Gm4487|Gm24073_chr14_114451568_R Primer3: not found at this Tm N.d. 0.04
mm4_intergenic_Dysf|Cyp26b1_chr6_84474591_F Primer3: not found at this Tm N.d. 0.04
mm4_intergenic_Dysf|Cyp26b1_chr6_84474591_R Primer3: not found at this Tm N.d. 0.04
mm3_exon_Gm26545_chr12_80885687_F TCGTCGGCAGCGTCTGTAGATGAAGCCTGCCAGC 60.1 0.04
mm3_exon_Gm26545_chr12_80885687_R GTCTCGTGGGCTCGGCCCCGAAATGAGGCAGCATA 60.1 0.04
mm4_intron_Scg5_chr2_113799105_F Primer3: not found at this Tm N.d. 0.03
mm4_intron_Scg5_chr2_113799105_R Primer3: not found at this Tm N.d. 0.03
mm4_intergenic_Lrpap1|Adra2c_chr5_35248229_F Primer3: not found at this Tm N.d. 0.03
mm4_intergenic_Lrpap1|Adra2c_chr5_35248229_R Primer3: not found at this Tm N.d. 0.03
mm4_intergenic_Ear6|Mettl17_chr14_51866882_F Primer3: not found at this Tm N.d. 0.03
mm4_intergenic_Ear6|Mettl17_chr14_51866882_R Primer3: not found at this Tm N.d. 0.03
mm4_intergenic_Rsbn1l|Ptpn12_chr5_20975538_F Primer3: not found at this Tm N.d. 0.03
mm4_intergenic_Rsbn1l|Ptpn12_chr5_20975538_R Primer3: not found at this Tm N.d. 0.03
mm3_exon_Efnb2_chr8_8620317_F TCGTCGGCAGCGTCTCTCCTAAGATGCTGCTGCG 59.8 0.03
mm3_exon_Efnb2_chr8_8620317_R GTCTCGTGGGCTCGGGAGATCCCCACTTGGACTGC 60.1 0.03
mm4_intergenic_1700008P02Rik|Gm22074_chr3_6971478_F Primer3: not found at this Tm N.d. 0.03
mm4_intergenic_1700008P02Rik|Gm22074_chr3_6971478_R Primer3: not found at this Tm N.d. 0.03
mm4_intergenic_Gm23041|Nav3_chr10_109534630_F Primer3: not found at this Tm N.d. 0.03
mm4_intergenic_Gm23041|Nav3_chr10_109534630_R Primer3: not found at this Tm N.d. 0.03
mm3_intergenic_Hmg20a|Gm26868_chr9_56503737_F Primer3: not found at this Tm N.d. 0.02
mm3_intergenic_Hmg20a|Gm26868_chr9_56503737_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_St6gal1|Gm23430_chr16_23394800_F TCGTCGGCAGCGTCACAAGAACCAAAGAGAGCCCC 60.2 0.02
mm4_intergenic_St6gal1|Gm23430_chr16_23394800_R GTCTCGTGGGCTCGGTGTGTTCGGTTGTCCATGTGA 60.1 0.02
mm4_intergenic_Gm14224|Epb4.1l1_chr2_156460996_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Gm14224|Epb4.1l1_chr2_156460996_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Gm22593|Mettl4_chr17_94706024_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Gm22593|Mettl4_chr17_94706024_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Adra2a|Gpam_chr19_54704433_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Adra2a|Gpam_chr19_54704433_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Ccdc130|Gm23626_chr8_84362613_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Ccdc130|Gm23626_chr8_84362613_R Primer3: not found at this Tm N.d. 0.02
mm4_intron_Zfp442_chr2_150427092_F Primer3: not found at this Tm N.d. 0.02
mm4_intron_Zfp442_chr2_150427092_R Primer3: not found at this Tm N.d. 0.02
mm4_intron_Vmn2r60_chr7_42166972_F Primer3: not found at this Tm N.d. 0.02
mm4_intron_Vmn2r60_chr7_42166972_R Primer3: not found at this Tm N.d. 0.02
mm4_intron_Tanc2_chr11_105815770_F Primer3: not found at this Tm N.d. 0.02
mm4_intron_Tanc2_chr11_105815770_R Primer3: not found at this Tm N.d. 0.02
mm4_intron_Spopl_chr2_23533667_F Primer3: not found at this Tm N.d. 0.02
mm4_intron_Spopl_chr2_23533667_R Primer3: not found at this Tm N.d. 0.02
mm4_intron_Sntg1_chr1_8709427_F Primer3: not found at this Tm N.d. 0.02
mm4_intron_Sntg1_chr1_8709427_R Primer3: not found at this Tm N.d. 0.02
mm4_intron_Abca13_chr11_9455573_F Primer3: not found at this Tm N.d. 0.02
mm4_intron_Abca13_chr11_9455573_R Primer3: not found at this Tm N.d. 0.02
mm4_intron_5830411N06Rik_chr7_140291387_F Primer3: not found at this Tm N.d. 0.02
mm4_intron_5830411N06Rik_chr7_140291387_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_n-R5s209|Gm7846_chr1_26992819_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_n-R5s209|Gm7846_chr1_26992819_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Vmn2r60|Vmn2r61_chr7_42201791_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Vmn2r60|Vmn2r61_chr7_42201791_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Vmn1r29|Gm20674_chr6_58359114_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Vmn1r29|Gm20674_chr6_58359114_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Vmn1r238|Crem_chr18_3205692_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Vmn1r238|Crem_chr18_3205692_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Ttc32|Gm7099_chr12_9320551_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Ttc32|Gm7099_chr12_9320551_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Tbx15|Gm12449_chr3_99391902_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Tbx15|Gm12449_chr3_99391902_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_St3gal1|Zfat_chr15_67870235_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_St3gal1|Zfat_chr15_67870235_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Spag11a|Defb14_chr8_19181248_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Spag11a|Defb14_chr8_19181248_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Slc22a29|Slc22a30_chr19_8327358_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Slc22a29|Slc22a30_chr19_8327358_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Slc22a27|Slc22a28_chr19_8042450_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Slc22a27|Slc22a28_chr19_8042450_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Sema3d|Gm23243_chr5_12799666_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Sema3d|Gm23243_chr5_12799666_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Lmo7|Gm22347_chr14_102285404_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Lmo7|Gm22347_chr14_102285404_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Itgbl1|Gm9308_chr14_123674037_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Itgbl1|Gm9308_chr14_123674037_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Has2os|Gm26178_chr15_57194014_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Has2os|Gm26178_chr15_57194014_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Gtpbp4|Larp4b_chr13_9048183_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Gtpbp4|Larp4b_chr13_9048183_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Gpc5|Gpc6_chr14_116888946_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Gpc5|Gpc6_chr14_116888946_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Gm884|Gm11643_chr11_103596518_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Gm884|Gm11643_chr11_103596518_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Gm7191|Gm23494_chr8_97833631_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Gm7191|Gm23494_chr8_97833631_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Gm6367|Gm7942_chr5_95094844_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Gm6367|Gm7942_chr5_95094844_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Gm6205|Gm3183_chr5_94764601_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Gm6205|Gm3183_chr5_94764601_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Gm5257|mmu-mir-6343_chr1_76234207_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Gm5257|mmu-mir-6343_chr1_76234207_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Gm3259|D5Ertd577e_chr5_95412315_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Gm3259|D5Ertd577e_chr5_95412315_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Gm26341|Amd2_chr10_35309631_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Gm26341|Amd2_chr10_35309631_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Gm25756|Gm22553_chr5_79203628_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Gm25756|Gm22553_chr5_79203628_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Gm23950|Gm11218_chr4_64737949_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Gm23950|Gm11218_chr4_64737949_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Gm23848|Trps1_chr15_50204384_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Gm23848|Trps1_chr15_50204384_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Gm23476|Gm26159_chr16_8270883_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Gm23476|Gm26159_chr16_8270883_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Gm23189|Gm15671_chr1_68015976_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Gm23189|Gm15671_chr1_68015976_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Gm22438|Gm22159_chr1_108428923_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Gm22438|Gm22159_chr1_108428923_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Gm21447|Spin2f_chrX_31229611_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Gm21447|Spin2f_chrX_31229611_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Gm17615|Fgf14_chr14_124009446_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Gm17615|Fgf14_chr14_124009446_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Gm16685|Gm24614_chr3_7832932_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Gm16685|Gm24614_chr3_7832932_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Gm13506|Gm13454_chr2_39425994_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Gm13506|Gm13454_chr2_39425994_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Gm13504|Vmn2r-ps2_chr2_39265670_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Gm13504|Vmn2r-ps2_chr2_39265670_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Gm11746|Slc24a3_chr2_144979411_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Gm11746|Slc24a3_chr2_144979411_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_GM6367|AC123873.1_chr5_JH584299_random_76907_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_GM6367|AC123873.1_chr5_JH584299_random_76907_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Eya1|Msc_chr1_14388601_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Eya1|Msc_chr1_14388601_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Csgalnact1|Ints10_chr8_68759754_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Csgalnact1|Ints10_chr8_68759754_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Cdh9|Gm22032_chr15_16863885_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Cdh9|Gm22032_chr15_16863885_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_C87414|AC140365.1_chr5_JH584299_random_703858_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_C87414|AC140365.1_chr5_JH584299_random_703858_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_B430218F22Rik/Mrps30|Fgf10_chr13_118463510_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_B430218F22Rik/Mrps30|Fgf10_chr13_118463510_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_AC126035.1|Gm16367_chr5_JH584299_random_383381_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_AC126035.1|Gm16367_chr5_JH584299_random_383381_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_A830012C17Rik|Gm11780_chr4_4479069_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_A830012C17Rik|Gm11780_chr4_4479069_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Gm26784|1700018B08Rik_chr8_121523584_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Gm26784|1700018B08Rik_chr8_121523584_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Pinc|Gm25329_chr1_73609295_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Pinc|Gm25329_chr1_73609295_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Skint11|Trabd2b_chr4_114255181_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Skint11|Trabd2b_chr4_114255181_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Gm12813|Skint5_chr4_113404871_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Gm12813|Skint5_chr4_113404871_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Cplx1|Gak_chr5_108552068_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Cplx1|Gak_chr5_108552068_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Gm4419|Gm5633_chr12_21512728_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Gm4419|Gm5633_chr12_21512728_R Primer3: not found at this Tm N.d. 0.02
mm4_intron_Add2_chr6_86110503_F TCGTCGGCAGCGTCCTAGCCCCTGAGAACAAGCC 60.1 0.02
mm4_intron_Add2_chr6_86110503_R GTCTCGTGGGCTCGGCAAGCACAGATGGAGGCAGA 60.0 0.02
mm4_intergenic_4931408C20Rik|n-R5s209_chr1_26709176_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_4931408C20Rik|n-R5s209_chr1_26709176_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Cdh7|Cdh19_chr1_110717926_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Cdh7|Cdh19_chr1_110717926_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Gm26327|Gm6851_chr7_39765113_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Gm26327|Gm6851_chr7_39765113_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Spanxn4|Gm7763_chr12_62689618_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Spanxn4|Gm7763_chr12_62689618_R Primer3: not found at this Tm N.d. 0.02
mm4_intron_Rarb_chr14_16474134_F Primer3: not found at this Tm N.d. 0.02
mm4_intron_Rarb_chr14_16474134_R Primer3: not found at this Tm N.d. 0.02
mm4_intron_Pbrm1_chr14_31096764_F Primer3: not found at this Tm N.d. 0.02
mm4_intron_Pbrm1_chr14_31096764_R Primer3: not found at this Tm N.d. 0.02
mm4_intron_Naip3_chr13_100366695_F Primer3: not found at this Tm N.d. 0.02
mm4_intron_Naip3_chr13_100366695_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Rbfox1|Gm23476_chr16_7816943_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Rbfox1|Gm23476_chr16_7816943_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Kcnk12|Rpl36-ps4_chr17_87806199_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Kcnk12|Rpl36-ps4_chr17_87806199_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Gm25491|Eya1_chr1_14024694_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Gm25491|Eya1_chr1_14024694_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Gm25051|Gm1818_chr12_47730521_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Gm25051|Gm1818_chr12_47730521_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Gm22782|Rpl10l_chr12_66067245_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Gm22782|Rpl10l_chr12_66067245_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Gm22062|Gm15224_chrX_165839454_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Gm22062|Gm15224_chrX_165839454_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Gm12610|Dmrta1_chr4_89495079_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Gm12610|Dmrta1_chr4_89495079_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Fam110b|Gm11797_chr4_5896118_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Fam110b|Gm11797_chr4_5896118_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Dcx|Gm15048_chrX_144086532_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Dcx|Gm15048_chrX_144086532_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Bambi|Gm23643_chr18_3751556_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Bambi|Gm23643_chr18_3751556_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_AC112986.1|Tsn_chr1_118186786_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_AC112986.1|Tsn_chr1_118186786_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_AC112986.1|Tsn_chr1_117975191_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_AC112986.1|Tsn_chr1_117975191_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Il27|Nupr1_chr7_126614642_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Il27|Nupr1_chr7_126614642_R Primer3: not found at this Tm N.d. 0.02
mm4_intron_Dtnb_chr12_3653373_F Primer3: not found at this Tm N.d. 0.02
mm4_intron_Dtnb_chr12_3653373_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Ythdf3|Gm26485_chr3_16437336_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Ythdf3|Gm26485_chr3_16437336_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Vmn1r69|Vmn1r70_chr7_10622067_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Vmn1r69|Vmn1r70_chr7_10622067_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Slitrk2|Gm23000_chrX_66723352_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Slitrk2|Gm23000_chrX_66723352_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Rfc3|Gm24242_chr5_151676661_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Rfc3|Gm24242_chr5_151676661_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Rarb|Thrb_chr14_17281252_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Rarb|Thrb_chr14_17281252_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Gm7719|Gm24377_chr12_59650965_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Gm7719|Gm24377_chr12_59650965_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Gm26820|Tacr3_chr3_134795922_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Gm26820|Tacr3_chr3_134795922_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Gm26820|Tacr3_chr3_134787584_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Gm26820|Tacr3_chr3_134787584_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Gm24790|Klf6_chr13_5383344_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Gm24790|Klf6_chr13_5383344_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Gm23228|Fbxo4_chr15_3889063_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Gm23228|Fbxo4_chr15_3889063_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Gm23097|Dync2h1_chr9_6770071_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Gm23097|Dync2h1_chr9_6770071_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Fbxo15|Gm25453_chr18_85643750_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Fbxo15|Gm25453_chr18_85643750_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_4930415L06Rik|Gm6027_chrX_89942443_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_4930415L06Rik|Gm6027_chrX_89942443_R Primer3: not found at this Tm N.d. 0.02
mm4_intron_Acvr2a_chr2_48856548_F Primer3: not found at this Tm N.d. 0.01
mm4_intron_Acvr2a_chr2_48856548_R Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Trav15n-1|Trav9n-2_chr14_53182979_F Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Trav15n-1|Trav9n-2_chr14_53182979_R Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Olfr892-ps1|Olfr893_chr9_38204649_F Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Olfr892-ps1|Olfr893_chr9_38204649_R Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Gm26190|B3gat1_chr9_26510004_F Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Gm26190|B3gat1_chr9_26510004_R Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Gm18367|Gm20713/Gpc5_chr14_115707217_F Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Gm18367|Gm20713/Gpc5_chr14_115707217_R Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Arl15|Ndufs4_chr13_114247000_F Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Arl15|Ndufs4_chr13_114247000_R Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Gm7405|Dach2_chrX_113277264_F Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Gm7405|Dach2_chrX_113277264_R Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Lpl|Slc18a1_chr8_68916573_F Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Lpl|Slc18a1_chr8_68916573_R Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Gm12458|Gm25485_chr4_50105228_F Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Gm12458|Gm25485_chr4_50105228_R Primer3: not found at this Tm N.d. 0.01
mm4_intron_Ppapdc1a_chr7_129277182_F Primer3: not found at this Tm N.d. 0.01
mm4_intron_Ppapdc1a_chr7_129277182_R Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Gm23117|Gm5083_chr13_43936733_F Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Gm23117|Gm5083_chr13_43936733_R Primer3: not found at this Tm N.d. 0.01
mm4_intron_Lrrc48_chr11_60382342_F TCGTCGGCAGCGTCCCATTCGCACCTTCTGAGGA 59.7 0.01
mm4_intron_Lrrc48_chr11_60382342_R GTCTCGTGGGCTCGGCAGCTTCTTGTGGGCTGAGA 59.9 0.01
mm4_intron_March1_chr8_66241592_F Primer3: not found at this Tm N.d. 0.01
mm4_intron_March1_chr8_66241592_R Primer3: not found at this Tm N.d. 0.01
mm4_intron_Atg10_chr13_91148020_F Primer3: not found at this Tm N.d. 0.01
mm4_intron_Atg10_chr13_91148020_R Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Gm25415|Gm10110_chr14_89721186_F Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Gm25415|Gm10110_chr14_89721186_R Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Gm5839|Gm25440_chr18_49373802_F Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Gm5839|Gm25440_chr18_49373802_R Primer3: not found at this Tm N.d. 0.01
mm4_intron_Vmn2r-ps69_chr7_85322035_F Primer3: not found at this Tm N.d. 0.01
mm4_intron_Vmn2r-ps69_chr7_85322035_R Primer3: not found at this Tm N.d. 0.01
mm4_intron_Ass1_chr2_31509697_F TCGTCGGCAGCGTCGCGTGTTCATGCATGTGTGT 60.0 0.01
mm4_intron_Ass1_chr2_31509697_R GTCTCGTGGGCTCGGGGCCTGCAGTTGAAAACCTG 59.9 0.01
mm4_intergenic_Zcchc6|Gas1_chr13_60014352_F Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Zcchc6|Gas1_chr13_60014352_R Primer3: not found at this Tm N.d. 0.01
mm4_intron_Prodh2_chr7_30500996_F Primer3: not found at this Tm N.d. 0.01
mm4_intron_Prodh2_chr7_30500996_R Primer3: not found at this Tm N.d. 0.01
mm4_intron_Ndufaf5_chr2_140184141_F Primer3: not found at this Tm N.d. 0.01
mm4_intron_Ndufaf5_chr2_140184141_R Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Gm25551|Jakmip1_chr5_37098922_F Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Gm25551|Jakmip1_chr5_37098922_R Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Casp12|Pdgfd_chr9_6061091_F Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Casp12|Pdgfd_chr9_6061091_R Primer3: not found at this Tm N.d. 0.01
mm3_intergenic_Capn8|4922505E12Rik_chr1_182728006_F Primer3: not found at this Tm N.d. 0.01
mm3_intergenic_Capn8|4922505E12Rik_chr1_182728006_R Primer3: not found at this Tm N.d. 0.01
mm4_intron_Cntnap5a_chr1_116050723_F Primer3: not found at this Tm N.d. 0.01
mm4_intron_Cntnap5a_chr1_116050723_R Primer3: not found at this Tm N.d. 0.01
mm4_intron_Cd36_chr5_17792011_F Primer3: not found at this Tm N.d. 0.01
mm4_intron_Cd36_chr5_17792011_R Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Tpbpa|Ctsj_chr13_60993163_F Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Tpbpa|Ctsj_chr13_60993163_R Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Tpbpa|Ctsj_chr13_60984957_F Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Tpbpa|Ctsj_chr13_60984957_R Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Tpbpa|Ctsj_chr13_60970303_F Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Tpbpa|Ctsj_chr13_60970303_R Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Tmem65|Ube2d4_chr15_58837032_F Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Tmem65|Ube2d4_chr15_58837032_R Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Tmem108|Nphp3_chr9_103799894_F Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Tmem108|Nphp3_chr9_103799894_R Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Stc2|Bod1_chr11_31587766_F Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Stc2|Bod1_chr11_31587766_R Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Spata4|Wdr17_chr8_54625000_F Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Spata4|Wdr17_chr8_54625000_R Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Rpl7a-ps10|Trim42/Gm15891_chr9_97219677_F Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Rpl7a-ps10|Trim42/Gm15891_chr9_97219677_R Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Rgs1|Rgs21_chr1_144476994_F Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Rgs1|Rgs21_chr1_144476994_R Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Pdzrn4|Gm4335_chr15_92885552_F Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Pdzrn4|Gm4335_chr15_92885552_R Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Macc1|Gm25675_chr12_119857780_F Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Macc1|Gm25675_chr12_119857780_R Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Kcnt2|Gm4845_chr1_141126871_F Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Kcnt2|Gm4845_chr1_141126871_R Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Kcnh5|Rhoj_chr12_75194612_F Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Kcnh5|Rhoj_chr12_75194612_R Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Ikzf2|Spag16_chr1_69805727_F Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Ikzf2|Spag16_chr1_69805727_R Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Hnf4g|Gm23237_chr3_3540156_F Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Hnf4g|Gm23237_chr3_3540156_R Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Gpr149|Gm22433_chr3_62637145_F Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Gpr149|Gm22433_chr3_62637145_R Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Gm5849|4930529C04Rik_chr3_90893744_F Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Gm5849|4930529C04Rik_chr3_90893744_R Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Gm5471|Kcnv1_chr15_45037369_F Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Gm5471|Kcnv1_chr15_45037369_R Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Gm26913|Gm4076_chr13_84989156_F Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Gm26913|Gm4076_chr13_84989156_R Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Gm26181|Triml1_chr8_43097649_F Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Gm26181|Triml1_chr8_43097649_R Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Gm24246|Pik3c3_chr18_28343151_F Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Gm24246|Pik3c3_chr18_28343151_R Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Gm22762|4930405D11Rik_chr11_90794258_F Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Gm22762|4930405D11Rik_chr11_90794258_R Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Gm22444|mmu-mir-6238_chr7_53051481_F Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Gm22444|mmu-mir-6238_chr7_53051481_R Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Gm17131|Tmem132d_chr5_127939438_F Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Gm17131|Tmem132d_chr5_127939438_R Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Gm13566|Gm13567_chr2_60589715_F Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Gm13566|Gm13567_chr2_60589715_R Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Gm13365|Gm13368_chr2_15403605_F Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Gm13365|Gm13368_chr2_15403605_R Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Gm12551|Plin2_chr4_86642311_F Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Gm12551|Plin2_chr4_86642311_R Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Gm12377|Gm12376_chr3_36805258_F Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Gm12377|Gm12376_chr3_36805258_R Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Gm11228|Gm11229_chr4_71155499_F Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Gm11228|Gm11229_chr4_71155499_R Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Fbxo15|Gm25453_chr18_85919044_F Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Fbxo15|Gm25453_chr18_85919044_R Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Fbxo15|Gm25453_chr18_85517094_F Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Fbxo15|Gm25453_chr18_85517094_R Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Cd180|Mast4_chr13_102723501_F Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Cd180|Mast4_chr13_102723501_R Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Car8|Gm11810_chr4_8256760_F Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Car8|Gm11810_chr4_8256760_R Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Cdh8|Gm15680_chr8_99294886_F Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Cdh8|Gm15680_chr8_99294886_R Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Pebp4|Egr3_chr14_70075565_F TCGTCGGCAGCGTCCCGGGGATCCTCTCTTCCTT 60.3 0.01
mm4_intergenic_Pebp4|Egr3_chr14_70075565_R GTCTCGTGGGCTCGGGATTCCCTCCTCAGTGGCC 59.4 0.01
mm4_intron_Ppp1r9a_chr6_5110139_F TCGTCGGCAGCGTCGCATCACTTAGTGGCTGTGC 59.5 0.01
mm4_intron_Ppp1r9a_chr6_5110139_R GTCTCGTGGGCTCGGTGAAATTCCCAATTCTCCCGGT 59.9 0.01
mm4_intergenic_A330008L17Rik|Gm15210_chr8_99796818_F Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_A330008L17Rik|Gm15210_chr8_99796818_R Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Gm6594|Pkdcc_chr17_83108353_F Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Gm6594|Pkdcc_chr17_83108353_R Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Gm11515|Tac4_chr11_95226386_F TCGTCGGCAGCGTCGCCTGGCTGTCATCTCAGAA 59.7 0.01
mm4_intergenic_Gm11515|Tac4_chr11_95226386_R GTCTCGTGGGCTCGGAGGCATCTGCTGTGATCTGG 59.8 0.01
mm4_intergenic_Vrk1|Gm24986_chr12_106199160_F Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Vrk1|Gm24986_chr12_106199160_R Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Gm12178|Gm12179_chr11_48331481_F Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Gm12178|Gm12179_chr11_48331481_R Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Gm8919|Gm8935_chr3_11774957_F Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Gm8919|Gm8935_chr3_11774957_R Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_5033428I22Rik|Scrg1_chr8_57377914_F Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_5033428I22Rik|Scrg1_chr8_57377914_R Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Stmn4|Gm23899_chr14_66456800_F TCGTCGGCAGCGTCAGTGCAAGTCTTTCCCTCCA 58.8 0.01
mm4_intergenic_Stmn4|Gm23899_chr14_66456800_R GTCTCGTGGGCTCGGGTGGTAATGGAATCTGGTCCCA 59.7 0.01
mm4_intron_Ptrf_chr11_100965309_F Primer3: not found at this Tm N.d. 0.01
mm4_intron_Ptrf_chr11_100965309_R Primer3: not found at this Tm N.d. 0.01
mm4_exon_Ttc17_chr2_94358187_F Primer3: not found at this Tm N.d. 0.00
mm4_exon_Ttc17_chr2_94358187_R Primer3: not found at this Tm N.d. 0.00
mm4_intron_Stx2_chr5_128990044_F Primer3: not found at this Tm N.d. 0.00
mm4_intron_Stx2_chr5_128990044_R Primer3: not found at this Tm N.d. 0.00
mm4_intron_Gm14066_chr2_139347230_F Primer3: not found at this Tm N.d. 0.00
mm4_intron_Gm14066_chr2_139347230_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm9726/mmu-mir-8099-2|Gm26055_chr12_95036392_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm9726/mmu-mir-8099-2|Gm26055_chr12_95036392_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Helt|Acsl1_chr8_46469134_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Helt|Acsl1_chr8_46469134_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Wwc1|Gm12125_chr11_35985201_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Wwc1|Gm12125_chr11_35985201_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Ccdc171|Gm27046_chr4_83589213_F TCGTCGGCAGCGTCCAGGGAGCAGCCTTAGGAAC 60.1 0.00
mm4_intergenic_Ccdc171|Gm27046_chr4_83589213_R GTCTCGTGGGCTCGGCTTACAAGGCCACCCTCCTG 60.0 0.00
mm4_exon_Pinlyp_chr7_24542589_F Primer3: not found at this Tm N.d. 0.00
mm4_exon_Pinlyp_chr7_24542589_R Primer3: not found at this Tm N.d. 0.00
mm4_intron_Xrra1_chr7_99872360_F Primer3: not found at this Tm N.d. 0.00
mm4_intron_Xrra1_chr7_99872360_R Primer3: not found at this Tm N.d. 0.00
mm4_intron_Wscd2_chr5_113497178_F Primer3: not found at this Tm N.d. 0.00
mm4_intron_Wscd2_chr5_113497178_R Primer3: not found at this Tm N.d. 0.00
mm4_intron_Txnrd2_chr16_18473476_F Primer3: not found at this Tm N.d. 0.00
mm4_intron_Txnrd2_chr16_18473476_R Primer3: not found at this Tm N.d. 0.00
mm4_intron_Tiam1_chr16_89972188_F Primer3: not found at this Tm N.d. 0.00
mm4_intron_Tiam1_chr16_89972188_R Primer3: not found at this Tm N.d. 0.00
mm4_intron_Shisa9_chr16_12024465_F Primer3: not found at this Tm N.d. 0.00
mm4_intron_Shisa9_chr16_12024465_R Primer3: not found at this Tm N.d. 0.00
mm4_intron_Sept8_chr11_53536569_F Primer3: not found at this Tm N.d. 0.00
mm4_intron_Sept8_chr11_53536569_R Primer3: not found at this Tm N.d. 0.00
mm4_intron_Plxna4_chr6_32227659_F Primer3: not found at this Tm N.d. 0.00
mm4_intron_Plxna4_chr6_32227659_R Primer3: not found at this Tm N.d. 0.00
mm4_intron_Nfat5_chr8_107294013_F Primer3: not found at this Tm N.d. 0.00
mm4_intron_Nfat5_chr8_107294013_R Primer3: not found at this Tm N.d. 0.00
mm4_intron_Msi2_chr11_88548267_F TCGTCGGCAGCGTCTTTCAGCAAGGCCAGAGGAG 59.9 0.00
mm4_intron_Msi2_chr11_88548267_R GTCTCGTGGGCTCGGGCAGGCTTCTACGAGTCTGC 60.8 0.00
mm4_intron_Mef2b/2310045N01Rik_chr8_70158608_F Primer3: not found at this Tm N.d. 0.00
mm4_intron_Mef2b/2310045N01Rik_chr8_70158608_R Primer3: not found at this Tm N.d. 0.00
mm4_intron_Etfa_chr9_55482901_F Primer3: not found at this Tm N.d. 0.00
mm4_intron_Etfa_chr9_55482901_R Primer3: not found at this Tm N.d. 0.00
mm4_intron_Elp4_chr2_105751874_F Primer3: not found at this Tm N.d. 0.00
mm4_intron_Elp4_chr2_105751874_R Primer3: not found at this Tm N.d. 0.00
mm4_intron_Cux1_chr5_136305408_F Primer3: not found at this Tm N.d. 0.00
mm4_intron_Cux1_chr5_136305408_R Primer3: not found at this Tm N.d. 0.00
mm4_intron_Atp7b_chr8_21997164_F Primer3: not found at this Tm N.d. 0.00
mm4_intron_Atp7b_chr8_21997164_R Primer3: not found at this Tm N.d. 0.00
mm4_intron_Actn1_chr12_80258861_F TCGTCGGCAGCGTCTGAGCATTCTCCCTCAAGCC 59.7 0.00
mm4_intron_Actn1_chr12_80258861_R GTCTCGTGGGCTCGGAACACCCAACTCCCGTTCAG 60.1 0.00
mm4_intron_4930584F24Rik_chr5_26462682_F Primer3: not found at this Tm N.d. 0.00
mm4_intron_4930584F24Rik_chr5_26462682_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Wfdc18|1100001G20Rik_chr11_83711395_F TCGTCGGCAGCGTCTCCTTAGCATCCTTCCTGTTGA 58.8 0.00
mm4_intergenic_Wfdc18|1100001G20Rik_chr11_83711395_R GTCTCGTGGGCTCGGAGGGCTCTGTCTGTGTTTATGG 60.0 0.00
mm4_intergenic_Tlr11|Olfr736_chr14_50367200_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Tlr11|Olfr736_chr14_50367200_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Tbx5|n-R5s176_chr5_120033702_F TCGTCGGCAGCGTCTGACCTTAGCCTTCTGTCCTTG 59.6 0.00
mm4_intergenic_Tbx5|n-R5s176_chr5_120033702_R GTCTCGTGGGCTCGGACACAGGCATCACTCAGTGG 59.9 0.00
mm4_intergenic_Phyh|Gm13194_chr2_4941056_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Phyh|Gm13194_chr2_4941056_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Olfr1219|Olfr1220_chr2_89093618_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Olfr1219|Olfr1220_chr2_89093618_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Mapk8|Ptpn20_chr14_33486794_F TCGTCGGCAGCGTCTAGAGACCTGGGCCCAACTT 60.1 0.00
mm4_intergenic_Mapk8|Ptpn20_chr14_33486794_R GTCTCGTGGGCTCGGATTGAGGAGCTCTGCCCTTG 59.7 0.00
mm4_intergenic_Lmnb2|Gadd45b_chr10_80926821_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Lmnb2|Gadd45b_chr10_80926821_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm8730|Cdh5_chr8_103737423_F TCGTCGGCAGCGTCGACAGCATTGTAGAGATGGGGT 59.8 0.00
mm4_intergenic_Gm8730|Cdh5_chr8_103737423_R GTCTCGTGGGCTCGGGAGGGCTGCTTCTCTCCAAG 60.1 0.00
mm4_intergenic_Gm4913|Gm16420_chrX_120050501_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm4913|Gm16420_chrX_120050501_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm25845|Celf4_chr18_25627881_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm25845|Celf4_chr18_25627881_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm25564|Dcdc2c_chr12_28389804_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm25564|Dcdc2c_chr12_28389804_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm24415|Alg10b/mmu-mir-7648_chr15_90214730_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm24415|Alg10b/mmu-mir-7648_chr15_90214730_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm23015|4932441J04Rik_chr5_57553985_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm23015|4932441J04Rik_chr5_57553985_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm21856|Gm21783_chrY_44793979_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm21856|Gm21783_chrY_44793979_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm21783|Gm21779_chrY_45199068_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm21783|Gm21779_chrY_45199068_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm21774|Gm21805_chrY_42114885_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm21774|Gm21805_chrY_42114885_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm20901|Gm21865_chrY_39188431_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm20901|Gm21865_chrY_39188431_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm18822|Hey1_chr3_8626293_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm18822|Hey1_chr3_8626293_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm15747|Mlxip_chr5_123416489_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm15747|Mlxip_chr5_123416489_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm14868|Gm4784_chrX_109288828_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm14868|Gm4784_chrX_109288828_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Cryaa|Sik1_chr17_31748177_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Cryaa|Sik1_chr17_31748177_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Cadm2|Gm24681_chr16_68130313_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Cadm2|Gm24681_chr16_68130313_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_2410088K16Rik|Sh3bp4_chr1_89014498_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_2410088K16Rik|Sh3bp4_chr1_89014498_R Primer3: not found at this Tm N.d. 0.00
mm4_exon_Hmga1_chr17_27559663_F Primer3: not found at this Tm N.d. 0.00
mm4_exon_Hmga1_chr17_27559663_R Primer3: not found at this Tm N.d. 0.00
mm4_exon_Hmga1-rs1_chr11_120762983_F Primer3: not found at this Tm N.d. 0.00
mm4_exon_Hmga1-rs1_chr11_120762983_R Primer3: not found at this Tm N.d. 0.00
mm4_exon_E230001N04Rik_chr17_28524085_F TCGTCGGCAGCGTCTGGCGCTATTAATGTCAGCT 57.3 0.00
mm4_exon_E230001N04Rik_chr17_28524085_R GTCTCGTGGGCTCGGCACTTCTGTAACCTCGCCCC 60.3 0.00
mm4_exon_Dbnl_chr11_5793630_F Primer3: not found at this Tm N.d. 0.00
mm4_exon_Dbnl_chr11_5793630_R Primer3: not found at this Tm N.d. 0.00
mm4_exon_4930563D23Rik_chr16_92321425_F TCGTCGGCAGCGTCGTTTGTGAAGCGAGCCATGG 60.1 0.00
mm4_exon_4930563D23Rik_chr16_92321425_R GTCTCGTGGGCTCGGGCCCCTGACACGTAGCTATG 60.2 0.00
mm3_intron_Havcr1_chr11_46736728_F Primer3: not found at this Tm N.d. 0.00
mm3_intron_Havcr1_chr11_46736728_R Primer3: not found at this Tm N.d. 0.00
mm2_intron_Sufu_chr19_46477782_F TCGTCGGCAGCGTCAGTTGCTGTGCTAGGAGCTG 60.0 0.00
mm2_intron_Sufu_chr19_46477782_R GTCTCGTGGGCTCGGCTTGGTGATCAAGGGTGCGT 60.6 0.00

Off-target amplicon sequences with primers

These only list off-targets that have primers in the table above. Primers underlined, off-targets in bold.

ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52313825 AGGGAGCCTGGGATTAGGAGCTTACTTGGGGGTTTTCCCCCTCCCCCCCTCTGGAGAGTCCGGGGATGGAGAGCCGAAAG
GACATGGTTATGTTTCTGGATGGGGGTCAGCTTGGCACTCTGGTTGGTA
mm2_intergenic_Rxrb|Col11a2_chr17_34039129 GATTACGAGTGGGCGTGGAACGGTCGCCCGTAGGCCCGCCCCTTCTGTCCCACTGTGCCCTGAGTCCCTCCCCGGACTCT
TCATAGGG
GCTCTGGGCCCCCAAGGGCAGCTGCCGTGGTGTGAAAGGACTA
mm3_intron_Btbd16_chr7_130797858 TGCTTCTGGCTTTGACCCTTAGGTGTGTAATCTAGCCGCACCATCTCATGGTCTGACACAGCCCCACAGGGACCTTCCTC
GGACTCTCCACAGAG
GTGTAGCCACACTGTGCATGTGTCCTCCT
mm4_intron_Kctd3_chr1_188988363 ATGGGGCTGGGTTTTCCAAAGGTCCACAGGCACTGTCACGCTGGAGAATCCGAGGACGGTAAGAAAGGAGAGTGTGGTCT
AAGGGACATCCACACTAAGATGAGAGTTACACTTCTGGCTCCTCTCAGGT
mm4_intergenic_Zfp335|Gm11458/Zfp335_chr2_164911234 CGGGGTCAGTGGTTCACTCCCCCTCCCCGGACTCCCCTGCGGAGGGGGCTGACAGCGACGGTCGCGGGGCCTGGTGTCTC
GGGGCTGTC
mm4_intergenic_Cers3|Adamts17_chr7_66835373 GACCATGGAATCAAGCCGGAAGAACAAAAGAGAGTCAAAGTGATGGGCTTCCCCACTTTTATTCTTGGCAAGCTTCCTTC
TGGAGGCTCCAGGGAAGG
AGAATTCGATAGCCATGTAACTTCACCCTCAGATAGCCCCA
mm4_intron_Col9a2_chr4_121040107 GCTATTCAGTCGACAGCGGAGCCTCCGCCCCCGGAGTCTCCTAAGGGACCCAGGCTCCAGCCAGACTTCCCTGTCGCCCC
AATATG
mm3_intergenic_Gm14246|Gm22936_chr2_162742448 GTGACCACTTACAGGCAGCTCCCTGCAGACTGGAGAGATTCCCTCGCACATCCCCAGATCCCCTCTGCCACACAAGCCCT
CCCTTGCCTTCTCCTGACTCTCCAGAGGCCACAGCAATTAGCTCCAGACAGTTCTCACGA
mm4_intron_Ablim2_chr5_35794655 GCATAGGCCTTAGGGTTGCACCATGCAGGCCCCCCAGAACCCTCTCCTGACTCTCCTGATGGTGCTTCCAAGCCTTGGCA
TGGCTGGCCTGGGCCCATTCAGCTGCTCTTGGCCTCAGGTTAC
mm4_intron_Unc13c_chr9_73952449 GGCATGACTGAGTTGGGTCACTTGAAGATTCCTTCAATATTGTTACTCCTTTCCCTGACTTTCCAGAGTGCTGGTTGGTC
CCCAAGGGCTCACACTGAGTCACTCTCTGAGAATGCTGCTTCCTACTCTGGGTG
mm4_intron_Adcy7_chr8_88313430 GACTGCTGACTCCCATCCAGGGCCCTCCTTCCCCGAACACTCCTGTGGGGACCCAGTTTCACCCTTGGTCAGCCCCCCTC
TGCACCTTCCCTCTTCCTTCC
mm4_intron_Map3k10_chr7_27661707 GTACTGCAGGGTGGTAAGCATAGAGAAAGGCTCTCTGTAGAGACAGGGGATGGCAGTAGCGGTCAGTGCTTCA
mm4_intergenic_Fbxo21|Tesc_chr5_118010562 ACACCGCTAGGCTACTCAGAATGAGCCCTCTCCACACCTGCAGCTTGCTGGCCCTCTGGAGAGGCCAGACAGGGGGACGG
GTCTTCCTCAGTCAAGCC
mm4_intergenic_Zfp652|Phospho1_chr11_95780681 ACACCCAGCAGAAATCTTGGATCTTGAGGGAGAAACATGGGTAGAAACAGCTGGACTCCCTCCGTGGACACTCCAGAGAG
GAATGTGCCTTTGGTGGCCAGCGTGGTGCAACTGTGTCCGACGTTCTATGCA
mm3_intergenic_Gm24371|BC024582_chr4_32540354 ACAGACTGTGTCAGGAACGTTCACTCAGAGATTCTCCAACCTTCCCCTGACACTCCAGAGAGATCATGGAAGTATGGGGA
GAGAGGAGACAGATAAGGCATTAGCAGGAAGGAGCATACATGGGCAAGGACC
mm3_intergenic_Gm9843|Usp25_chr16_76470165 CTGTTTTCTGGCTGTGTTGGGTCCTCCCCGGACTTTCTAGATGGAGACCCATATCTAACTCTTATCCTTTTATATCTTGG
TATGATTGACCTTTGGTTATGTCATGCTTGCACTTGACAC
mm3_exon_Gm26545_chr12_80885687 TGTAGATGAAGCCTGCCAGCTGAGCTCAACATCTGGAGAGTCGGGGGAAGGGGAGGGGGCATGTGAAACAAGTTCTTTGG
TTCAGCACAAACACATCAGGCATATGCTGCCTCATTTCGGGG
mm3_exon_Efnb2_chr8_8620317 TCTCCTAAGATGCTGCTGCGCCATCCATAGCCTACCGTGTCCTCCCCGGGCTCTGCAGAGGCCTGAGTGGCTGGGTGCAT
GGCTTGGAGGTCCGCGTGGGGCCGCGGCAGTCCAAGTGGGGATCTC
mm4_intergenic_St6gal1|Gm23430_chr16_23394800 ACAAGAACCAAAGAGAGCCCCCCTTCCCCAGAGTATCCAGAGGTCAGCATGATAAATGGGTAAGCTAGAAGGAACTTTAA
AGGACCTGTGCTCTGATCAGACCACACTTTCTATATCACATGGACAACCGAACACA
mm4_intron_Add2_chr6_86110503 CTAGCCCCTGAGAACAAGCCCCAGGTGACCCTCTGGAGAGCCAGCGGTGGGGACTCTGCCTCCATCTGTGCTTG
mm4_intron_Lrrc48_chr11_60382342 CCATTCGCACCTTCTGAGGACCAGGCTGGGGCCTGACTCACCCTCGAAACAAGTCCCTCTGGAGAGGCAGGTCATGGACC
CCGCTAAACCTGACTCAGTGCCCTGCCTGTATCTCAGCCCACAAGAAGCTG
mm4_intron_Ass1_chr2_31509697 GCGTGTTCATGCATGTGTGTGCACAGCCGTCCCCCTCCCCCGACTCCCCAGTGGAAGGGAGGAAGGGCTATTTGGGGCCC
TGGGCTCCACCGTAGTTGGTTTAATTACAGGTTTTCAACTGCAGGCC
mm4_intergenic_Pebp4|Egr3_chr14_70075565 CCGGGGATCCTCTCTTCCTTCTTGTCAACACTCCCACTCCCCCTGAGAGATGCAAGTTCCCCCAACCCCGACTCTCCAGC
GGG
GGCCGCGGGATTCACCGCGCCCACGCGGCCACTGAGGAGGGAATC
mm4_intron_Ppp1r9a_chr6_5110139 GCATCACTTAGTGGCTGTGCTCAGCTGCAGAGCAGAAAATGTTCATGGGCTTGGCTGCAAAGTGTCTCCCTCCCCAGACC
CTCCACTGGG
CTGTTTGGACCTATAAAACCGGGAGAATTGGGAATTTCA
mm4_intergenic_Gm11515|Tac4_chr11_95226386 GCCTGGCTGTCATCTCAGAACTTCTAGAACTCCTGGTCCCTCCCCCGACTCCCCAGTGCGTGTTTCCCAGCCCCCTATTT
CCTCCCCAGATCACAGCAGATGCCT
mm4_intergenic_Stmn4|Gm23899_chr14_66456800 AGTGCAAGTCTTTCCCTCCATTTTTACAGACAAATAGCTTGCATGCCCGTCTCAGACTCTCCAGAGGGTCTGTATTAATT
GATTTGGGACCAGATTCCATTACCAC
mm4_intergenic_Ccdc171|Gm27046_chr4_83589213 CAGGGAGCAGCCTTAGGAACTAAACGCCTGCCCCTCTGGAGGGGAGGGGGAAGGGAGAGTGTTCTTCGATTGTCCTCCTG
ACCAGGAGGGTGGCCTTGTAAG
mm4_intron_Msi2_chr11_88548267 TTTCAGCAAGGCCAGAGGAGTGTGCCCGCTGGAGAGCCCCGGGGAGGATCGCCGAGGAAGCAGACTCGTAGAAGCCTGC
mm4_intron_Actn1_chr12_80258861 TGAGCATTCTCCCTCAAGCCCAGCCCTAACCGCACTATCCAGAGGGACGCGCCATCCTGTACACCCCCCCCCCCATTCCC
AGATCACGAAGTGGGGGGGGGGGGACTGAACGGGAGTTGGGTGTT
mm4_intergenic_Wfdc18|1100001G20Rik_chr11_83711395 TCCTTAGCATCCTTCCTGTTGAAGAGTTCTTATTTGTGTATCATATTGACTATTTTATTCACAAGAGCCATCCCTGCAGC
CTCCAGAGGG
TCCATAAACACAGACAGAGCCCT
mm4_intergenic_Tbx5|n-R5s176_chr5_120033702 TGACCTTAGCCTTCTGTCCTTGCACTCTGGAGAGTCCTGGATGGGTAGGGCCACTGCTTTCCTGCTGTGTCAGAATAGCA
ATTTTGGTCTGAACAAGTCCTCACCCACTGAGTGATGCCTGTGT
mm4_intergenic_Mapk8|Ptpn20_chr14_33486794 TAGAGACCTGGGCCCAACTTCCCTCCCAGGGCAATCCAGAGGGCTGCATGTCTCTCTAGAAGCCATGGGCCAAGGGCAGA
GCTCCTCAAT
mm4_intergenic_Gm8730|Cdh5_chr8_103737423 GACAGCATTGTAGAGATGGGGTATCTGAGACCTAAAACAAAGAAATCCTCTCTGGAAAGTGAGGGGAAGGTTGTTGCTTG
GAGAGAAGCAGCCCTC
mm4_exon_E230001N04Rik_chr17_28524085 TGGCGCTATTAATGTCAGCTTTTCAAAATANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNAGTGCGGGCAGGGCATGGGGGGCGAGGTTACAGAAGTG
mm4_exon_4930563D23Rik_chr16_92321425 GTTTGTGAAGCGAGCCATGGGGCTCCCTGAGTGGCTCACATTTTCCCATCTGGGGAGTTCTGGGAGGGGGAGAAGGATGA
CCTCACAAAGGGACATCTCACGCTGTACCATAGCTACGTGTCAGGGGC
mm2_intron_Sufu_chr19_46477782 AGTTGCTGTGCTAGGAGCTGCTTAATCAAAGGCCTGTTATGGGCTCATTAGTGTGGTCCACCACGGGCCACCCCTCAGGA
GAGTCCTGGGAAGG
CTGGCTTAGGTCCCGCCCTTCAGGCACGCACCCTTGATCACCAAG

Input file for Crispresso

Crispresso, written by Luca Pinello, is a software package to quantify the Cas9-induced mutations on off- or on-targets.

Click here to download an amplicon input file for Crispresso. For each off-target, it includes the off-target name, its PCR amplicon and the guide sequence. Keep a copy of this file.

After sequencing, run CRISPRessoPooled. The tool will map the reads to the amplicons and analyse the mutations:
CRISPRessoPooled -r1 Reads1.fastq.gz -r2 Reads2.fastq.gz -f crisporAmplicons_mN1FkRligAThyqKv3eg3.txt --name MY_EXPERIMENT

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CRISPR/Cas9 Guide Designer for chordate vertebrate ecdysozoans lophotrochozoans protostomes spongi corals plants butterflies metazoans genomes fruitflies insects nematodes mammals.