CRISPOR

DNA break repair outcome predictions

Guide: CCAGAGGCGGTGCGGCACAG AGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

mh- Score	Sequence	Effect
	`GAGGGCAATGGGGCTTCCTCCCAGAGGCGGTGCGGCACAGAGGAGCGCTCGCATCACAAGGTGACCCTAGCTCCCCACTG`	(Wild-type sequence)
313	`GGGCTTCCTCCCAGAGGCGGTGCG-------------CTCGCATCACAAGGTGACCCTAG`	`13 bp deleted → frameshift`
271	`GGGCTTCCTCCCAGAGGCGGTGCGGCACAG--GAGCGCTCGCATCACAAGGTGACCCTAG`	`2 bp deleted → frameshift`
268	`GGGCTTCCTCCCAGAGGCGGTGCGG--------AGCGCTCGCATCACAAGGTGACCCTAG`	`8 bp deleted → frameshift`
244	`GGGCTTCCTCCCAGAGGCG------------------CTCGCATCACAAGGTGACCCTAG`	`18 bp deleted → no frameshift`
242	`GGGCTTCCTCCCAGAGGCGGTGCGGC----------GCTCGCATCACAAGGTGACCCTAG`	`10 bp deleted → frameshift`
233	`GGGCTTCCTCCCAGAGGCGGTGCGGCACAG-----CGCTCGCATCACAAGGTGACCCTAG`	`5 bp deleted → frameshift`
232	`GGGCTTCCTCCCAGAGGCGGTGCGGCACA-------------------AGGTGACCCTAG`	`19 bp deleted → frameshift`
224	`GGGCTTCCTCCCAGAGGCGGTGCGGCA----------------TCACAAGGTGACCCTAG`	`16 bp deleted → frameshift`
224	`GGGCTTCCTCCCAGAGG----------------AGCGCTCGCATCACAAGGTGACCCTAG`	`16 bp deleted → frameshift`
219	`GGGCTTCCTCCCAGAGGCGGTGCGGC------------TCGCATCACAAGGTGACCCTAG`	`12 bp deleted → no frameshift`
208	`GGGCTTCCTCCCAGAGGCGG-------------AGCGCTCGCATCACAAGGTGACCCTAG`	`13 bp deleted → frameshift`
193	`GGGCTTCCTCCCAGAG-------------------CGCTCGCATCACAAGGTGACCCTAG`	`19 bp deleted → frameshift`
188	`GGGCTTCCTCCCAGAGGCGGTGC---------------TCGCATCACAAGGTGACCCTAG`	`15 bp deleted → no frameshift`
170	`GGGCTTCCTCCCAGAGGCGGTGCG-----------------CATCACAAGGTGACCCTAG`	`17 bp deleted → frameshift`
154	`GGGCTTCCTCCCAGAGGCGGTGC-------------------ATCACAAGGTGACCCTAG`	`19 bp deleted → frameshift`
149	`GGGCTTCCTCCCAGAGGCGGTGCGGCACA--------------TCACAAGGTGACCCTAG`	`14 bp deleted → frameshift`
148	`GGGCTTCCTCCCAGAGGCGGTG-------------------------------ACCCTAG`	`31 bp deleted → frameshift`
147	`GGGCTTCCTCCCAGAGGC--------------------TCGCATCACAAGGTGACCCTAG`	`20 bp deleted → frameshift`
133	`GGGCTTCCTCCCAGAGGCG----------------------CATCACAAGGTGACCCTAG`	`22 bp deleted → frameshift`
128	`GGGCTTCCTCCCAGAGGCGGTGCGGCACA-----------------CAAGGTGACCCTAG`	`17 bp deleted → frameshift`
122	`GGGCTTCCTCCCAG------------------GAGCGCTCGCATCACAAGGTGACCCTAG`	`18 bp deleted → no frameshift`
120	`GGGCTTCCTCCCAGAGGC------------------------ATCACAAGGTGACCCTAG`	`24 bp deleted → no frameshift`
111	`GGGCTTCCTC------------------------------GCATCACAAGGTGACCCTAG`	`30 bp deleted → no frameshift`
110	`GGGCTTCCTCCCAGAGGCGGTGCGGCACAG--------------------GTGACCCTAG`	`20 bp deleted → frameshift`
109	`GGGCTTCCTCCCAGAGGCGGTGCGG--------------------------TGACCCTAG`	`26 bp deleted → frameshift`
105	`GGGCTTCCTCCCAGAGGCGGTGCGGCA---------------------AGGTGACCCTAG`	`21 bp deleted → no frameshift`
105	`GGGCTTCCTCCCAG---------------------CGCTCGCATCACAAGGTGACCCTAG`	`21 bp deleted → no frameshift`
91	`GGGCTTCCTCCCAGAGG----------------------------------TGACCCTAG`	`34 bp deleted → frameshift`
91	`GGGCT----------------------------------CGCATCACAAGGTGACCCTAG`	`34 bp deleted → frameshift`
89	`GGG------------------------------AGCGCTCGCATCACAAGGTGACCCTAG`	`30 bp deleted → no frameshift`
84	`GG-------------------------------AGCGCTCGCATCACAAGGTGACCCTAG`	`31 bp deleted → frameshift`
80	`GGGC--------------------------------GCTCGCATCACAAGGTGACCCTAG`	`32 bp deleted → frameshift`
77	`GGGCTTCCTCCCAGAGGCGGTGCGGCAC---------------------------CCTAG`	`27 bp deleted → no frameshift`
66	`GGGCTTCCTCCCAGAGGCGGTGCGGCACAG------------------------------`	`30 bp deleted → no frameshift`
66	`GGGCTTCCTCCCA------------------------------TCACAAGGTGACCCTAG`	`30 bp deleted → no frameshift`
63	`GGGCTTCCTCCC---------------------------------------------TAG`	`45 bp deleted → no frameshift`
60	`GGGC--------------------------------------ATCACAAGGTGACCCTAG`	`38 bp deleted → frameshift`
57	`GGGCTTCCTCCCA---------------------------------CAAGGTGACCCTAG`	`33 bp deleted → no frameshift`
57	`GGGCTTC---------------------------------GCATCACAAGGTGACCCTAG`	`33 bp deleted → no frameshift`
52	`GGGCTTCCTCCCA-----------------------------------AGGTGACCCTAG`	`35 bp deleted → frameshift`
52	`GGGCTTCCTC-----------------------------------ACAAGGTGACCCTAG`	`35 bp deleted → frameshift`
49	`GGGCTTCCTCCCAG------------------------------------GTGACCCTAG`	`36 bp deleted → no frameshift`
45	`GGGCTTC--------------------------------------ACAAGGTGACCCTAG`	`38 bp deleted → frameshift`
44	`GGGCTTCCTCCC--------------------------------------------CTAG`	`44 bp deleted → frameshift`
43	`GGGCTTCCT-------------------------------------------------AG`	`49 bp deleted → frameshift`
42	`GGGCTTCCTCCCAGA---------------------------------------CCCTAG`	`39 bp deleted → no frameshift`
40	`GGGCTTCCTCC----------------------------------------------TAG`	`46 bp deleted → frameshift`
36	`GGGCTTCC------------------------------------------------CTAG`	`48 bp deleted → no frameshift`
36	`GGG------------------------------------------------TGACCCTAG`	`48 bp deleted → no frameshift`
34	`GG-------------------------------------------------TGACCCTAG`	`49 bp deleted → frameshift`
33	`GGGCTTCCTCCCAGAG--------------------------------------------`	`44 bp deleted → frameshift`
30	`GGGCTTCCTCCCAG----------------------------------------------`	`46 bp deleted → frameshift`
21	`GGGCT-----------------------------------------------------AG`	`53 bp deleted → frameshift`

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