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The following table lists possible deletions.
Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.
| mh- Score | Sequence | Effect |
|---|---|---|
| CTTGGCCAGAGAGGCTGTCCGCGGAGGGGACTGGGGGTCCCGGGCCAAGCATGGCCGCTCCAGCAGACCGTGGCTCGGCG | (Wild-type sequence) | |
| 423 | GAGGCTGTCCGCGGAGGGGACTGGGGG-------CCAAGCATGGCCGCTCCAGCAGACCG | 7 bp deleted → frameshift |
| 402 | GAGGCTGTCCGCGGAGGGGACTGGGG--------CCAAGCATGGCCGCTCCAGCAGACCG | 8 bp deleted → frameshift |
| 382 | GAGGCTGTCCGCGGAGGGGACTGGG---------CCAAGCATGGCCGCTCCAGCAGACCG | 9 bp deleted → no frameshift |
| 296 | GAGGCTGTCCGCGGAGGGGACTGGGGGTCC------AAGCATGGCCGCTCCAGCAGACCG | 6 bp deleted → no frameshift |
| 296 | GAGGCTGTCCGCGGAGGGGACTGGGGG------GCCAAGCATGGCCGCTCCAGCAGACCG | 6 bp deleted → no frameshift |
| 283 | GAGGCTGTCCGCGGAGGGG---------------CCAAGCATGGCCGCTCCAGCAGACCG | 15 bp deleted → no frameshift |
| 269 | GAGGCTGTCCGCGGAGGG----------------CCAAGCATGGCCGCTCCAGCAGACCG | 16 bp deleted → frameshift |
| 242 | GAGGCTGTCCGCGGAGGGGACTGG----------CCAAGCATGGCCGCTCCAGCAGACCG | 10 bp deleted → frameshift |
| 232 | GAGGCTGTCCGCGG-------------------GCCAAGCATGGCCGCTCCAGCAGACCG | 19 bp deleted → frameshift |
| 198 | GAGGCTGTCCGCGGAGGGG--------------GCCAAGCATGGCCGCTCCAGCAGACCG | 14 bp deleted → frameshift |
| 184 | GAGGCTGTCCGCGGAGGGGACTGG--------------------CCGCTCCAGCAGACCG | 20 bp deleted → frameshift |
| 179 | GAGGCTGTCCGCGGAGGGGACTGGGGGTCC----------------GCTCCAGCAGACCG | 16 bp deleted → frameshift |
| 175 | GAGGCTGTCCGCGGAGGGGACTGGGGGTCC---------------------AGCAGACCG | 21 bp deleted → no frameshift |
| 170 | GAGGCTGTCCGCGGAGGGGACTGGGGG-----------------CCGCTCCAGCAGACCG | 17 bp deleted → frameshift |
| 170 | GAGGCTGTCCGCGGAGG-----------------CCAAGCATGGCCGCTCCAGCAGACCG | 17 bp deleted → frameshift |
| 162 | GAGGCTGTCCGCGGAGGGGACTGGGG------------------CCGCTCCAGCAGACCG | 18 bp deleted → no frameshift |
| 154 | GAGGCTGTCCGCGGAGGGGACTGGG-------------------CCGCTCCAGCAGACCG | 19 bp deleted → frameshift |
| 147 | GAGGCTGTCCGCGG--------------------CCAAGCATGGCCGCTCCAGCAGACCG | 20 bp deleted → frameshift |
| 140 | GAGGCTGTCCG---------------------GGCCAAGCATGGCCGCTCCAGCAGACCG | 21 bp deleted → no frameshift |
| 133 | GAGGC------------------------------CAAGCATGGCCGCTCCAGCAGACCG | 30 bp deleted → no frameshift |
| 132 | GAGGCTGTCCGC------------------------------------TCCAGCAGACCG | 36 bp deleted → no frameshift |
| 126 | GAGGCTGTCCGC-----------------------CAAGCATGGCCGCTCCAGCAGACCG | 23 bp deleted → frameshift |
| 114 | GAGGCTGTCCGCGGAGGGG-------------------------CCGCTCCAGCAGACCG | 25 bp deleted → frameshift |
| 109 | GAGGCTGTCCGCGGAGGG--------------------------CCGCTCCAGCAGACCG | 26 bp deleted → frameshift |
| 109 | GAGGCTGTCC--------------------------AAGCATGGCCGCTCCAGCAGACCG | 26 bp deleted → frameshift |
| 103 | GAGGCTGTCCGCGGAGG---------------------------CCGCTCCAGCAGACCG | 27 bp deleted → no frameshift |
| 98 | GAGGCTGTCCGC----------------------------ATGGCCGCTCCAGCAGACCG | 28 bp deleted → frameshift |
| 95 | GAGGCTGTCCGCGGAG-----------------------CATGGCCGCTCCAGCAGACCG | 23 bp deleted → frameshift |
| 94 | GAGGCTGTCCGCGGAGGGGACTGGGGGTCC-----------------------------G | 29 bp deleted → frameshift |
| 94 | GAGG-----------------------------GCCAAGCATGGCCGCTCCAGCAGACCG | 29 bp deleted → frameshift |
| 89 | GAGGCTGTCCGCGG------------------------------CCGCTCCAGCAGACCG | 30 bp deleted → no frameshift |
| 81 | GAGGC----------------------------------------CGCTCCAGCAGACCG | 40 bp deleted → frameshift |
| 78 | GAGGCTGTCCGCGGAGGGGAC-------------------------------------CG | 37 bp deleted → frameshift |
| 77 | GAGGCTGTCCGCGGAGGGGACT---------------------------CCAGCAGACCG | 27 bp deleted → no frameshift |
| 76 | GAGGCTGTCCGC---------------------------------CGCTCCAGCAGACCG | 33 bp deleted → no frameshift |
| 73 | GAGGCTGTCCGCG----------------------------------CTCCAGCAGACCG | 34 bp deleted → frameshift |
| 69 | GAGGC-----------------------------------ATGGCCGCTCCAGCAGACCG | 35 bp deleted → frameshift |
| 64 | GAGGCTGTCC-----------------------------------------AGCAGACCG | 41 bp deleted → frameshift |
| 58 | GAGGCT-------------------------------------------CCAGCAGACCG | 43 bp deleted → frameshift |
| 51 | GAGGCTGTCCG------------------------------------------------- | 49 bp deleted → frameshift |
| 49 | GAGGCTG------------------------------------GCCGCTCCAGCAGACCG | 36 bp deleted → no frameshift |
| 49 | GAG------------------------------------CATGGCCGCTCCAGCAGACCG | 36 bp deleted → no frameshift |
| 48 | GAGGCTGTCCGC------------------------------------------AGACCG | 42 bp deleted → no frameshift |
| 47 | GAGGCTGTCCGCGGAG-------------------------------------CAGACCG | 37 bp deleted → frameshift |
| 40 | GAGGCTGTCCGCGGAG----------------------------------------ACCG | 40 bp deleted → frameshift |
| 38 | GAGGCTGTCCGCG----------------------------------------------- | 47 bp deleted → frameshift |
| 36 | GAGGCTGTCCGCGGA------------------------------------------CCG | 42 bp deleted → no frameshift |
| 34 | GAGGC-------------------------------------------------AGACCG | 49 bp deleted → frameshift |
| 24 | GAG--------------------------------------------------CAGACCG | 50 bp deleted → frameshift |
| 21 | GAG-----------------------------------------------------ACCG | 53 bp deleted → frameshift |
| 19 | GA-------------------------------------------------------CCG | 55 bp deleted → frameshift |