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Contents:In the table below, Illumina Nextera Handle sequences have been added and highlighted in bold. Primers for the on-target have been added for convenience. The table below is sorted by the CFD off-target score. Sites with very low CFD scores < 0.02 are unlikely to be cleaved, see our study Haeussler et al. 2016, Figure 2.
In the protocol by Matthew Canver, Harvard, two PCRs are run: one PCR to amplify the potential off-target, then a second PCR to extend the handles with Illumina barcodes. Please click here to download the protocol. Alternatively, you can have a look at Fu et al, 2014.
If a primer was not found, the reason is usually that the region around the off-target is too repetitive. To avoid unspecific primers, all repeats are masked for the primer design (not for off-target search). If you think that we should change the parameters here or should use different primer3 settings, please let us know.
| Name | Primer Sequence | Tm | CFD Score |
|---|---|---|---|
| ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52313943_F | TCGTCGGCAGCGTCAATTTGTCCGAAGCCGTGAG | 58.8 | 1.00 |
| ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52313943_R | GTCTCGTGGGCTCGGCTCCTCCTCCTGGCCTCC | 60.4 | 1.00 |
| mm3_intron_Mettl14_chr3_123383432_F | Primer3: not found at this Tm | N.d. | 0.74 |
| mm3_intron_Mettl14_chr3_123383432_R | Primer3: not found at this Tm | N.d. | 0.74 |
| mm4_intergenic_Gm12911|Brinp1_chr4_68422024_F | Primer3: not found at this Tm | N.d. | 0.66 |
| mm4_intergenic_Gm12911|Brinp1_chr4_68422024_R | Primer3: not found at this Tm | N.d. | 0.66 |
| mm4_intron_4933431K23Rik_chr8_77640042_F | Primer3: not found at this Tm | N.d. | 0.59 |
| mm4_intron_4933431K23Rik_chr8_77640042_R | Primer3: not found at this Tm | N.d. | 0.59 |
| mm4_intergenic_Calm1/Gm26723|Ttc7b_chr12_100221581_F | TCGTCGGCAGCGTCGTTTCCAGGAGGAGCTGTCC | 60.0 | 0.59 |
| mm4_intergenic_Calm1/Gm26723|Ttc7b_chr12_100221581_R | GTCTCGTGGGCTCGGCAGGAGTGGAGAACAGGCTG | 60.0 | 0.59 |
| mm4_exon_6530409C15Rik/Zfp800_chr6_28260539_F | TCGTCGGCAGCGTCAAGAAGGGAAAGGAGCTGCC | 59.9 | 0.55 |
| mm4_exon_6530409C15Rik/Zfp800_chr6_28260539_R | GTCTCGTGGGCTCGGACTCGCGTAATGCCAAAAGC | 59.8 | 0.55 |
| mm3_intergenic_Npr3|Gm24074_chr15_11957577_F | Primer3: not found at this Tm | N.d. | 0.54 |
| mm3_intergenic_Npr3|Gm24074_chr15_11957577_R | Primer3: not found at this Tm | N.d. | 0.54 |
| mm3_intergenic_Gm16565/Cd247|Gm23402_chr1_165801935_F | TCGTCGGCAGCGTCCCGGGGTTGCCCTTTAGAAA | 60.2 | 0.54 |
| mm3_intergenic_Gm16565/Cd247|Gm23402_chr1_165801935_R | GTCTCGTGGGCTCGGGGGAAACAGAGGCCGTGAAA | 60.5 | 0.54 |
| mm3_intron_Stac_chr9_111577163_F | Primer3: not found at this Tm | N.d. | 0.51 |
| mm3_intron_Stac_chr9_111577163_R | Primer3: not found at this Tm | N.d. | 0.51 |
| mm4_intergenic_Gm23038|4930570G19Rik_chr3_156339556_F | Primer3: not found at this Tm | N.d. | 0.49 |
| mm4_intergenic_Gm23038|4930570G19Rik_chr3_156339556_R | Primer3: not found at this Tm | N.d. | 0.49 |
| mm4_intergenic_Cldn4|Cldn3/Wbscr25_chr5_134980391_F | Primer3: not found at this Tm | N.d. | 0.47 |
| mm4_intergenic_Cldn4|Cldn3/Wbscr25_chr5_134980391_R | Primer3: not found at this Tm | N.d. | 0.47 |
| mm4_intergenic_5S_rRNA|Gcnt4_chr13_96914968_F | Primer3: not found at this Tm | N.d. | 0.45 |
| mm4_intergenic_5S_rRNA|Gcnt4_chr13_96914968_R | Primer3: not found at this Tm | N.d. | 0.45 |
| mm4_intergenic_Pxt1|Kctd20_chr17_28943039_F | Primer3: not found at this Tm | N.d. | 0.44 |
| mm4_intergenic_Pxt1|Kctd20_chr17_28943039_R | Primer3: not found at this Tm | N.d. | 0.44 |
| mm4_intergenic_Pdzrn3|Gm9871_chr6_101471761_F | Primer3: not found at this Tm | N.d. | 0.43 |
| mm4_intergenic_Pdzrn3|Gm9871_chr6_101471761_R | Primer3: not found at this Tm | N.d. | 0.43 |
| mm4_intergenic_Pacrg|A230009B12Rik_chr17_10438035_F | Primer3: not found at this Tm | N.d. | 0.37 |
| mm4_intergenic_Pacrg|A230009B12Rik_chr17_10438035_R | Primer3: not found at this Tm | N.d. | 0.37 |
| mm4_exon_Arhgap32_chr9_32259021_F | Primer3: not found at this Tm | N.d. | 0.34 |
| mm4_exon_Arhgap32_chr9_32259021_R | Primer3: not found at this Tm | N.d. | 0.34 |
| mm4_intron_Nck2_chr1_43519691_F | TCGTCGGCAGCGTCGTGTGTAAGCAGCAGACCCT | 59.9 | 0.33 |
| mm4_intron_Nck2_chr1_43519691_R | GTCTCGTGGGCTCGGACATGTCTCCTTTGCTGGGG | 59.9 | 0.33 |
| mm4_intergenic_Gm26619/Gm10260|Arhgef28_chr13_97874486_F | TCGTCGGCAGCGTCTCCAGACTAGATGGGGCAGC | 61.0 | 0.33 |
| mm4_intergenic_Gm26619/Gm10260|Arhgef28_chr13_97874486_R | GTCTCGTGGGCTCGGCTTGACCACGGCTCAGCTAA | 60.0 | 0.33 |
| mm4_intergenic_Pigm|Slamf9_chr1_172473951_F | Primer3: not found at this Tm | N.d. | 0.31 |
| mm4_intergenic_Pigm|Slamf9_chr1_172473951_R | Primer3: not found at this Tm | N.d. | 0.31 |
| mm4_exon_Phf2_chr13_48835227_F | Primer3: not found at this Tm | N.d. | 0.28 |
| mm4_exon_Phf2_chr13_48835227_R | Primer3: not found at this Tm | N.d. | 0.28 |
| mm4_intergenic_Herpud2|Sept7_chr9_25215561_F | Primer3: not found at this Tm | N.d. | 0.27 |
| mm4_intergenic_Herpud2|Sept7_chr9_25215561_R | Primer3: not found at this Tm | N.d. | 0.27 |
| mm4_intron_Tkt_chr14_30558292_F | Primer3: not found at this Tm | N.d. | 0.27 |
| mm4_intron_Tkt_chr14_30558292_R | Primer3: not found at this Tm | N.d. | 0.27 |
| mm3_exon_Gm24139_chr5_134204652_F | Primer3: not found at this Tm | N.d. | 0.27 |
| mm3_exon_Gm24139_chr5_134204652_R | Primer3: not found at this Tm | N.d. | 0.27 |
| mm4_intergenic_Tuba3b|Gm15704_chr6_145710161_F | Primer3: not found at this Tm | N.d. | 0.27 |
| mm4_intergenic_Tuba3b|Gm15704_chr6_145710161_R | Primer3: not found at this Tm | N.d. | 0.27 |
| mm4_intergenic_Kif6|Rftn1_chr17_49949625_F | Primer3: not found at this Tm | N.d. | 0.26 |
| mm4_intergenic_Kif6|Rftn1_chr17_49949625_R | Primer3: not found at this Tm | N.d. | 0.26 |
| mm4_intron_1700007B14Rik_chr8_75454199_F | Primer3: not found at this Tm | N.d. | 0.26 |
| mm4_intron_1700007B14Rik_chr8_75454199_R | Primer3: not found at this Tm | N.d. | 0.26 |
| mm4_intergenic_Nin|Abhd12b_chr12_70146500_F | Primer3: not found at this Tm | N.d. | 0.25 |
| mm4_intergenic_Nin|Abhd12b_chr12_70146500_R | Primer3: not found at this Tm | N.d. | 0.25 |
| mm4_intergenic_Chsy1/Gm26827|Lrrk1_chr7_66197313_F | TCGTCGGCAGCGTCTTTTGCTTCCTCTGGGGTGG | 60.1 | 0.25 |
| mm4_intergenic_Chsy1/Gm26827|Lrrk1_chr7_66197313_R | GTCTCGTGGGCTCGGATCTCTGACACCCCAGCTCT | 59.9 | 0.25 |
| mm4_intergenic_Zfp947|Gm4944_chr17_22174017_F | Primer3: not found at this Tm | N.d. | 0.24 |
| mm4_intergenic_Zfp947|Gm4944_chr17_22174017_R | Primer3: not found at this Tm | N.d. | 0.24 |
| mm4_intergenic_Vmn2r-ps117|Vmn2r97_chr17_18855498_F | Primer3: not found at this Tm | N.d. | 0.24 |
| mm4_intergenic_Vmn2r-ps117|Vmn2r97_chr17_18855498_R | Primer3: not found at this Tm | N.d. | 0.24 |
| mm4_intergenic_Gm15446|1010001B22Rik_chr5_109953554_F | Primer3: not found at this Tm | N.d. | 0.24 |
| mm4_intergenic_Gm15446|1010001B22Rik_chr5_109953554_R | Primer3: not found at this Tm | N.d. | 0.24 |
| mm4_intergenic_Cntn5|Gm25365_chr9_12545775_F | Primer3: not found at this Tm | N.d. | 0.24 |
| mm4_intergenic_Cntn5|Gm25365_chr9_12545775_R | Primer3: not found at this Tm | N.d. | 0.24 |
| mm4_intergenic_Krt78|Krt8_chr15_101979892_F | Primer3: not found at this Tm | N.d. | 0.24 |
| mm4_intergenic_Krt78|Krt8_chr15_101979892_R | Primer3: not found at this Tm | N.d. | 0.24 |
| mm4_intron_Vav3_chr3_109592851_F | Primer3: not found at this Tm | N.d. | 0.23 |
| mm4_intron_Vav3_chr3_109592851_R | Primer3: not found at this Tm | N.d. | 0.23 |
| mm4_intron_Oscar_chr7_3615852_F | TCGTCGGCAGCGTCCAGCACAGTAGACTTGCCCA | 59.9 | 0.23 |
| mm4_intron_Oscar_chr7_3615852_R | GTCTCGTGGGCTCGGAAGACAGATTGCTGGGTCGG | 60.0 | 0.23 |
| mm4_intron_Pard3b_chr1_62405286_F | TCGTCGGCAGCGTCAGCAGAGCACTTCAGGAAGG | 59.6 | 0.23 |
| mm4_intron_Pard3b_chr1_62405286_R | GTCTCGTGGGCTCGGGCGGCCCCATCTGGATTTAA | 60.4 | 0.23 |
| mm3_intergenic_Adcy5|Gm25179_chr16_35223477_F | TCGTCGGCAGCGTCTGGCTCTTTGTCTGCCTCTG | 59.9 | 0.23 |
| mm3_intergenic_Adcy5|Gm25179_chr16_35223477_R | GTCTCGTGGGCTCGGTCATTCCCAGGTGAAGCTGC | 60.3 | 0.23 |
| mm4_intron_Rasgrf2_chr13_92107970_F | Primer3: not found at this Tm | N.d. | 0.23 |
| mm4_intron_Rasgrf2_chr13_92107970_R | Primer3: not found at this Tm | N.d. | 0.23 |
| mm4_intron_Daf2_chr1_130417402_F | Primer3: not found at this Tm | N.d. | 0.22 |
| mm4_intron_Daf2_chr1_130417402_R | Primer3: not found at this Tm | N.d. | 0.22 |
| mm4_intergenic_Gm24335|Sfrp1_chr8_23298467_F | Primer3: not found at this Tm | N.d. | 0.22 |
| mm4_intergenic_Gm24335|Sfrp1_chr8_23298467_R | Primer3: not found at this Tm | N.d. | 0.22 |
| mm4_intergenic_Gm5697|Gm6462_chr1_28847528_F | Primer3: not found at this Tm | N.d. | 0.21 |
| mm4_intergenic_Gm5697|Gm6462_chr1_28847528_R | Primer3: not found at this Tm | N.d. | 0.21 |
| mm4_intron_Ski_chr4_155163409_F | Primer3: not found at this Tm | N.d. | 0.21 |
| mm4_intron_Ski_chr4_155163409_R | Primer3: not found at this Tm | N.d. | 0.21 |
| mm2_intergenic_Tmprss5|Gm4894_chr9_49168730_F | Primer3: not found at this Tm | N.d. | 0.21 |
| mm2_intergenic_Tmprss5|Gm4894_chr9_49168730_R | Primer3: not found at this Tm | N.d. | 0.21 |
| mm4_intron_Anapc5_chr5_122799805_F | Primer3: not found at this Tm | N.d. | 0.20 |
| mm4_intron_Anapc5_chr5_122799805_R | Primer3: not found at this Tm | N.d. | 0.20 |
| mm4_intergenic_Gata4|Blk_chr14_63340125_F | Primer3: not found at this Tm | N.d. | 0.20 |
| mm4_intergenic_Gata4|Blk_chr14_63340125_R | Primer3: not found at this Tm | N.d. | 0.20 |
| mm4_intron_Sipa1l1_chr12_82332792_F | TCGTCGGCAGCGTCCAGATTGGGAGCTGGTGGTG | 60.6 | 0.20 |
| mm4_intron_Sipa1l1_chr12_82332792_R | GTCTCGTGGGCTCGGGCTCCAGCAAAGCAGACAAC | 60.0 | 0.20 |
| mm4_intergenic_Degs2|Gm23872_chr12_108717274_F | Primer3: not found at this Tm | N.d. | 0.19 |
| mm4_intergenic_Degs2|Gm23872_chr12_108717274_R | Primer3: not found at this Tm | N.d. | 0.19 |
| mm4_intron_Slco3a1_chr7_74298122_F | TCGTCGGCAGCGTCTCCGTGTCTAGTACCCAGCT | 59.6 | 0.19 |
| mm4_intron_Slco3a1_chr7_74298122_R | GTCTCGTGGGCTCGGCCCTCAGAGCCTTTTACCACA | 59.6 | 0.19 |
| mm4_intergenic_Dcc|Gm25509_chr18_72549905_F | TCGTCGGCAGCGTCTCCCATTCTTTTCCTTCAGGACT | 59.3 | 0.19 |
| mm4_intergenic_Dcc|Gm25509_chr18_72549905_R | GTCTCGTGGGCTCGGTCAGCTATACCCAGAGGACAGA | 59.4 | 0.19 |
| mm4_exon_Hoxa5_chr6_52202720_F | Primer3: not found at this Tm | N.d. | 0.18 |
| mm4_exon_Hoxa5_chr6_52202720_R | Primer3: not found at this Tm | N.d. | 0.18 |
| mm4_intergenic_Hist1h2bc|Hist1h1t_chr13_23695191_F | TCGTCGGCAGCGTCTAGCCCTGAGACTGGCTGAT | 60.0 | 0.18 |
| mm4_intergenic_Hist1h2bc|Hist1h1t_chr13_23695191_R | GTCTCGTGGGCTCGGGCATGTGAGAGGCTCGCTAT | 59.9 | 0.18 |
| mm4_intergenic_Gm9244|Gm5592_chr7_41271281_F | Primer3: not found at this Tm | N.d. | 0.18 |
| mm4_intergenic_Gm9244|Gm5592_chr7_41271281_R | Primer3: not found at this Tm | N.d. | 0.18 |
| mm4_intergenic_Gm12413|Sh3gl2_chr4_85265544_F | Primer3: not found at this Tm | N.d. | 0.18 |
| mm4_intergenic_Gm12413|Sh3gl2_chr4_85265544_R | Primer3: not found at this Tm | N.d. | 0.18 |
| mm4_intergenic_Gm7488|Tbl1xr1_chr3_21574362_F | TCGTCGGCAGCGTCGCAGAAGCAAACTTATTTGGAGT | 57.8 | 0.18 |
| mm4_intergenic_Gm7488|Tbl1xr1_chr3_21574362_R | GTCTCGTGGGCTCGGTTGTCCAGTGTGACACGAGG | 59.8 | 0.18 |
| mm4_intergenic_Stx11|Sf3b5_chr10_12984887_F | Primer3: not found at this Tm | N.d. | 0.18 |
| mm4_intergenic_Stx11|Sf3b5_chr10_12984887_R | Primer3: not found at this Tm | N.d. | 0.18 |
| mm4_intron_Gpr142_chr11_114804214_F | Primer3: not found at this Tm | N.d. | 0.17 |
| mm4_intron_Gpr142_chr11_114804214_R | Primer3: not found at this Tm | N.d. | 0.17 |
| mm4_intergenic_Gm5942|Gm14897_chrX_114795605_F | Primer3: not found at this Tm | N.d. | 0.17 |
| mm4_intergenic_Gm5942|Gm14897_chrX_114795605_R | Primer3: not found at this Tm | N.d. | 0.17 |
| mm4_intron_Unc5b_chr10_60787797_F | TCGTCGGCAGCGTCGACACAGAACACTGCCTCCA | 59.8 | 0.17 |
| mm4_intron_Unc5b_chr10_60787797_R | GTCTCGTGGGCTCGGGAGCAACTTGGTGTCCTCCA | 59.8 | 0.17 |
| mm4_intergenic_Gm23043|Gm24943_chr4_51679437_F | Primer3: not found at this Tm | N.d. | 0.16 |
| mm4_intergenic_Gm23043|Gm24943_chr4_51679437_R | Primer3: not found at this Tm | N.d. | 0.16 |
| mm4_intron_Gxylt2_chr6_100798847_F | Primer3: not found at this Tm | N.d. | 0.16 |
| mm4_intron_Gxylt2_chr6_100798847_R | Primer3: not found at this Tm | N.d. | 0.16 |
| mm4_intron_Mmel1_chr4_154870900_F | TCGTCGGCAGCGTCGGAGGACAGCAGAGGTCTTG | 59.7 | 0.16 |
| mm4_intron_Mmel1_chr4_154870900_R | GTCTCGTGGGCTCGGTTTAGATGCTCACTCCCCGC | 59.8 | 0.16 |
| mm4_exon_Ank3_chr10_69822562_F | Primer3: not found at this Tm | N.d. | 0.15 |
| mm4_exon_Ank3_chr10_69822562_R | Primer3: not found at this Tm | N.d. | 0.15 |
| mm4_exon_Adam22_chr5_8367298_F | Primer3: not found at this Tm | N.d. | 0.15 |
| mm4_exon_Adam22_chr5_8367298_R | Primer3: not found at this Tm | N.d. | 0.15 |
| mm4_exon_LRRC8A/Lrrc8a_chr2_30257172_F | Primer3: not found at this Tm | N.d. | 0.15 |
| mm4_exon_LRRC8A/Lrrc8a_chr2_30257172_R | Primer3: not found at this Tm | N.d. | 0.15 |
| mm4_intron_Cwh43_chr5_73410613_F | Primer3: not found at this Tm | N.d. | 0.15 |
| mm4_intron_Cwh43_chr5_73410613_R | Primer3: not found at this Tm | N.d. | 0.15 |
| mm4_intron_Bzw2_chr12_36138828_F | Primer3: not found at this Tm | N.d. | 0.14 |
| mm4_intron_Bzw2_chr12_36138828_R | Primer3: not found at this Tm | N.d. | 0.14 |
| mm4_intergenic_Gm14336|Gm14337_chr2_173926952_F | Primer3: not found at this Tm | N.d. | 0.14 |
| mm4_intergenic_Gm14336|Gm14337_chr2_173926952_R | Primer3: not found at this Tm | N.d. | 0.14 |
| mm3_intergenic_Gm14077|Macrod2_chr2_141344208_F | Primer3: not found at this Tm | N.d. | 0.14 |
| mm3_intergenic_Gm14077|Macrod2_chr2_141344208_R | Primer3: not found at this Tm | N.d. | 0.14 |
| mm3_intergenic_Ctsll3|4930486L24Rik_chr13_60826267_F | Primer3: not found at this Tm | N.d. | 0.14 |
| mm3_intergenic_Ctsll3|4930486L24Rik_chr13_60826267_R | Primer3: not found at this Tm | N.d. | 0.14 |
| mm3_intergenic_Gm23351|A530084C06Rik/Foxq1_chr13_31504551_F | TCGTCGGCAGCGTCCATACCCGAGAGCCATGGTC | 59.9 | 0.13 |
| mm3_intergenic_Gm23351|A530084C06Rik/Foxq1_chr13_31504551_R | GTCTCGTGGGCTCGGCTGGCCTGGGATCATGGTTT | 60.0 | 0.13 |
| mm4_intron_Csmd1_chr8_16314690_F | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_intron_Csmd1_chr8_16314690_R | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_intron_Dym_chr18_75060341_F | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_intron_Dym_chr18_75060341_R | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_intron_Ulk4_chr9_121030314_F | TCGTCGGCAGCGTCCCGATGGGTTGTTGGAGAGG | 60.3 | 0.12 |
| mm4_intron_Ulk4_chr9_121030314_R | GTCTCGTGGGCTCGGTGGACCTTCCCACAGTTTGG | 59.8 | 0.12 |
| mm4_intron_Cela1_chr15_100675272_F | TCGTCGGCAGCGTCTATCCAGCCTGGGGTTTCCT | 60.2 | 0.12 |
| mm4_intron_Cela1_chr15_100675272_R | GTCTCGTGGGCTCGGCCACCGTCTTCACCAGAGTC | 60.0 | 0.12 |
| mm4_intergenic_Mrgprb3|Zdhhc13_chr7_48757622_F | Primer3: not found at this Tm | N.d. | 0.12 |
| mm4_intergenic_Mrgprb3|Zdhhc13_chr7_48757622_R | Primer3: not found at this Tm | N.d. | 0.12 |
| mm4_intergenic_Gm6673|Gm3495_chr5_17277357_F | Primer3: not found at this Tm | N.d. | 0.12 |
| mm4_intergenic_Gm6673|Gm3495_chr5_17277357_R | Primer3: not found at this Tm | N.d. | 0.12 |
| mm3_intron_Pi4k2a_chr19_42092277_F | Primer3: not found at this Tm | N.d. | 0.12 |
| mm3_intron_Pi4k2a_chr19_42092277_R | Primer3: not found at this Tm | N.d. | 0.12 |
| mm4_exon_Aktip_chr8_91131990_F | Primer3: not found at this Tm | N.d. | 0.12 |
| mm4_exon_Aktip_chr8_91131990_R | Primer3: not found at this Tm | N.d. | 0.12 |
| mm4_exon_Acss2_chr2_155562058_F | Primer3: not found at this Tm | N.d. | 0.12 |
| mm4_exon_Acss2_chr2_155562058_R | Primer3: not found at this Tm | N.d. | 0.12 |
| mm4_intron_Sh3kbp1_chrX_159848708_F | Primer3: not found at this Tm | N.d. | 0.11 |
| mm4_intron_Sh3kbp1_chrX_159848708_R | Primer3: not found at this Tm | N.d. | 0.11 |
| mm4_intron_Als2_chr1_59198981_F | TCGTCGGCAGCGTCAGCTAAAACAAGAGCCCGGA | 59.3 | 0.11 |
| mm4_intron_Als2_chr1_59198981_R | GTCTCGTGGGCTCGGTGGCTTCTACAGAAAGACGGT | 59.0 | 0.11 |
| mm4_intergenic_Gm5152|Gm17091_chr5_10321267_F | Primer3: not found at this Tm | N.d. | 0.11 |
| mm4_intergenic_Gm5152|Gm17091_chr5_10321267_R | Primer3: not found at this Tm | N.d. | 0.11 |
| mm4_intron_Scn11a_chr9_119825373_F | TCGTCGGCAGCGTCAAAGGGGCTTGATCTCAGCC | 60.0 | 0.11 |
| mm4_intron_Scn11a_chr9_119825373_R | GTCTCGTGGGCTCGGGCAGGAAGACAGGTAAGCCA | 59.6 | 0.11 |
| mm4_intergenic_Hmgb1-ps9|Pik3r1_chr13_101585678_F | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intergenic_Hmgb1-ps9|Pik3r1_chr13_101585678_R | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intergenic_Gm11747|Gm11748/Engase_chr11_118469955_F | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intergenic_Gm11747|Gm11748/Engase_chr11_118469955_R | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intergenic_Gm12295|Gm26128_chr11_65393797_F | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intergenic_Gm12295|Gm26128_chr11_65393797_R | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intergenic_Gm23217|Gm24390_chr15_72477298_F | TCGTCGGCAGCGTCTTCCCAGTCTGAGGCCCTAA | 59.8 | 0.09 |
| mm4_intergenic_Gm23217|Gm24390_chr15_72477298_R | GTCTCGTGGGCTCGGGCTTGATCACACGTAAGCAGG | 59.6 | 0.09 |
| mm4_exon_Traf5_chr1_191997663_F | TCGTCGGCAGCGTCTGGGGTCAGCTTTGAAGGTC | 59.8 | 0.09 |
| mm4_exon_Traf5_chr1_191997663_R | GTCTCGTGGGCTCGGGTACTTTGTGGTGATGCGCG | 60.1 | 0.09 |
| mm4_intergenic_Pcnxl4|Dhrs7_chr12_72628905_F | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intergenic_Pcnxl4|Dhrs7_chr12_72628905_R | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intron_Speer7-ps1_chr5_15695113_F | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intron_Speer7-ps1_chr5_15695113_R | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intron_Gria4_chr9_4522235_F | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intron_Gria4_chr9_4522235_R | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intergenic_Pabpc2|Yipf5_chr18_39919108_F | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intergenic_Pabpc2|Yipf5_chr18_39919108_R | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intergenic_Gm17019|Gm21847_chr5_15512409_F | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intergenic_Gm17019|Gm21847_chr5_15512409_R | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intergenic_Gm14774|Il1rapl1_chrX_88050785_F | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intergenic_Gm14774|Il1rapl1_chrX_88050785_R | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intergenic_Gm14628|Gm14604_chrX_44086533_F | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intergenic_Gm14628|Gm14604_chrX_44086533_R | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intergenic_Trappc10|Agpat3_chr10_78249357_F | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intergenic_Trappc10|Agpat3_chr10_78249357_R | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intergenic_Klk5|Klk4_chr7_43866688_F | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intergenic_Klk5|Klk4_chr7_43866688_R | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_exon_Gm23875_chr12_32354971_F | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_exon_Gm23875_chr12_32354971_R | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_exon_Gm22261_chr2_147250562_F | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_exon_Gm22261_chr2_147250562_R | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intergenic_4930435H24Rik|Qdpr_chr5_45239452_F | TCGTCGGCAGCGTCGCACGGTAGGTCGTAATGGA | 59.5 | 0.08 |
| mm4_intergenic_4930435H24Rik|Qdpr_chr5_45239452_R | GTCTCGTGGGCTCGGTGGCCTTTAACTGATTCCCTT | 57.1 | 0.08 |
| mm4_intron_Ak7_chr12_105775323_F | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intron_Ak7_chr12_105775323_R | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intron_Armc2_chr10_41935926_F | TCGTCGGCAGCGTCTTGTTGCCCCTGTGGAAGTC | 60.4 | 0.07 |
| mm4_intron_Armc2_chr10_41935926_R | GTCTCGTGGGCTCGGCCTGGGTGAAGTGAGCTGAG | 60.0 | 0.07 |
| mm4_intergenic_Ube3b|Mmab_chr5_114430909_F | TCGTCGGCAGCGTCTCTGCTCAGCCTGGACAAAG | 59.9 | 0.07 |
| mm4_intergenic_Ube3b|Mmab_chr5_114430909_R | GTCTCGTGGGCTCGGCCAAGCTGGTCCTGTCTTGA | 59.6 | 0.07 |
| mm4_intergenic_Zfp946|Vmn2r111_chr17_22481823_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_Zfp946|Vmn2r111_chr17_22481823_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_D830050J10Rik/Raf1|Gm24008_chr6_115692293_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_D830050J10Rik/Raf1|Gm24008_chr6_115692293_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_Trps1|Eif3h_chr15_51203218_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_Trps1|Eif3h_chr15_51203218_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intron_Csmd2_chr4_128059502_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intron_Csmd2_chr4_128059502_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_exon_Gm11634_chr11_101595597_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_exon_Gm11634_chr11_101595597_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_Gm12728|Gm24363_chr4_105872975_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_Gm12728|Gm24363_chr4_105872975_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intron_C77080_chr4_129234749_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intron_C77080_chr4_129234749_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm3_intron_Zfpm2_chr15_41007376_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm3_intron_Zfpm2_chr15_41007376_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_4930584F24Rik|Dpp6_chr5_26710367_F | TCGTCGGCAGCGTCGAGCTGAGGTCATGCTGTGT | 60.0 | 0.05 |
| mm4_intergenic_4930584F24Rik|Dpp6_chr5_26710367_R | GTCTCGTGGGCTCGGCACACCGCAGAGGCTGATAT | 59.8 | 0.05 |
| mm4_intergenic_Gm16205|Sgcz_chr8_37927206_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_Gm16205|Sgcz_chr8_37927206_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_exon_Kif13b_chr14_64772900_F | TCGTCGGCAGCGTCCACATCAGGGCAGTTCTCGA | 59.7 | 0.04 |
| mm4_exon_Kif13b_chr14_64772900_R | GTCTCGTGGGCTCGGGAGTTGAGGACAGCTGTGCA | 60.2 | 0.04 |
| mm4_intergenic_Gm10797|Zfp365_chr10_67599606_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Gm10797|Zfp365_chr10_67599606_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Dnah5|Mir3964_chr15_28559479_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Dnah5|Mir3964_chr15_28559479_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intron_Sympk_chr7_19027655_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intron_Sympk_chr7_19027655_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intron_Ap2a1_chr7_44925737_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intron_Ap2a1_chr7_44925737_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intron_Aqr_chr2_114146809_F | TCGTCGGCAGCGTCTGAGGTTAGCAAAGGGCCAG | 59.9 | 0.03 |
| mm4_intron_Aqr_chr2_114146809_R | GTCTCGTGGGCTCGGGCCCGAGATTCCTTGGTCAA | 60.0 | 0.03 |
| mm4_intron_Plxna4_chr6_32153326_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intron_Plxna4_chr6_32153326_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Banf2|Gm12357_chr2_144047188_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Banf2|Gm12357_chr2_144047188_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_4932429P05Rik|Gm7108_chrX_89773672_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_4932429P05Rik|Gm7108_chrX_89773672_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm3_intron_Ptgds_chr2_25468389_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm3_intron_Ptgds_chr2_25468389_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm3_intergenic_Gm13851|9430029A11Rik_chr6_32412347_F | TCGTCGGCAGCGTCGCCTGGGAGACACCTGAAAA | 59.8 | 0.01 |
| mm3_intergenic_Gm13851|9430029A11Rik_chr6_32412347_R | GTCTCGTGGGCTCGGCACCAGGGCCAATTGGAAGA | 60.2 | 0.01 |
| mm4_intron_Prr5l_chr2_101753931_F | TCGTCGGCAGCGTCTCACCCACCCTCCCTATCAG | 60.0 | 0.01 |
| mm4_intron_Prr5l_chr2_101753931_R | GTCTCGTGGGCTCGGGAAGGAGGGGACTTCCATGC | 60.1 | 0.01 |
| mm4_exon_Fbrsl1_chr5_110379580_F | TCGTCGGCAGCGTCGCCTTGGCCTCTGACTTCTC | 60.3 | 0.01 |
| mm4_exon_Fbrsl1_chr5_110379580_R | GTCTCGTGGGCTCGGAAGCGCCAGGTCTTACAACA | 59.8 | 0.01 |
| mm4_intron_Wbscr17_chr5_131077567_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Wbscr17_chr5_131077567_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Tbc1d30_chr10_121293339_F | TCGTCGGCAGCGTCTAGAGGCACAGGTCCACCTT | 60.1 | 0.00 |
| mm4_intron_Tbc1d30_chr10_121293339_R | GTCTCGTGGGCTCGGCCTGTGCCTGTACAGTCCAT | 59.3 | 0.00 |
| mm4_intron_Slc23a2_chr2_132087141_F | TCGTCGGCAGCGTCCACCTGGAGTCACTTGCACT | 59.8 | 0.00 |
| mm4_intron_Slc23a2_chr2_132087141_R | GTCTCGTGGGCTCGGCAAGCTGGACCTGGGATGTG | 60.6 | 0.00 |
| mm4_intron_Relb_chr7_19618408_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Relb_chr7_19618408_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Nrcam_chr12_44492190_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Nrcam_chr12_44492190_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Lactb_chr9_66965888_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Lactb_chr9_66965888_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Kctd16_chr18_40496394_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Kctd16_chr18_40496394_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Igf2bp3_chr6_49193217_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Igf2bp3_chr6_49193217_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Gtf2ird2_chr5_134192488_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Gtf2ird2_chr5_134192488_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Fmn2_chr1_174751680_F | TCGTCGGCAGCGTCGTGAGAACAGTGGCTCCCTC | 60.0 | 0.00 |
| mm4_intron_Fmn2_chr1_174751680_R | GTCTCGTGGGCTCGGTCGGGAGCAGACAGTACTGA | 59.9 | 0.00 |
| mm4_intron_Entpd8_chr2_25082735_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Entpd8_chr2_25082735_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Eif4g3_chr4_138153088_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Eif4g3_chr4_138153088_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Cyth3_chr5_143641308_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Cyth3_chr5_143641308_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Adk_chr14_21319523_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Adk_chr14_21319523_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_A530013C23Rik_chr2_167707210_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_A530013C23Rik_chr2_167707210_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Ramp2|Ramp2/Vps25_chr11_101252280_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Ramp2|Ramp2/Vps25_chr11_101252280_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Prkag2|1500035N22Rik_chr5_24979815_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Prkag2|1500035N22Rik_chr5_24979815_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Pdia5|Sema5b_chr16_35539455_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Pdia5|Sema5b_chr16_35539455_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_P2ry2|Fchsd2_chr7_101078431_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_P2ry2|Fchsd2_chr7_101078431_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Nol10|Odc1_chr12_17449726_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Nol10|Odc1_chr12_17449726_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Manba|Nfkb1_chr3_135581328_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Manba|Nfkb1_chr3_135581328_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Lypd1/Gpr39|Lypd1_chr1_125896140_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Lypd1/Gpr39|Lypd1_chr1_125896140_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Lin7b|Snrnp70_chr7_45373180_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Lin7b|Snrnp70_chr7_45373180_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Kif26b|Gm16564_chr1_178891958_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Kif26b|Gm16564_chr1_178891958_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Kcnv1|Gm5472_chr15_45361306_F | TCGTCGGCAGCGTCTGGTGCTGCACAATTGTTGG | 59.8 | 0.00 |
| mm4_intergenic_Kcnv1|Gm5472_chr15_45361306_R | GTCTCGTGGGCTCGGACTTTCCTCCACATTCCCCTG | 59.6 | 0.00 |
| mm4_intergenic_Il12rb1|Arrdc2_chr8_70823783_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Il12rb1|Arrdc2_chr8_70823783_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm7931|Slit2_chr5_46607917_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm7931|Slit2_chr5_46607917_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm13828|Pop5_chr5_115200375_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm13828|Pop5_chr5_115200375_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm12764|Gm12766_chr7_34405116_F | TCGTCGGCAGCGTCTTTAAGCTCACGGGACCTG | 57.0 | 0.00 |
| mm4_intergenic_Gm12764|Gm12766_chr7_34405116_R | GTCTCGTGGGCTCGGTGTCACTAAGCCCCTCCC | 57.8 | 0.00 |
| mm4_intergenic_Gm12746|Ssbp3_chr4_106862967_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm12746|Ssbp3_chr4_106862967_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Commd10|Gm25036_chr18_47094619_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Commd10|Gm25036_chr18_47094619_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_4921515E04Rik|C030047K22Rik_chr15_18459781_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_4921515E04Rik|C030047K22Rik_chr15_18459781_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_1700031F10Rik|Eefsec_chr6_88250995_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_1700031F10Rik|Eefsec_chr6_88250995_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_exon_Prkab2/Gm17651_chr3_97671823_F | TCGTCGGCAGCGTCGGTGGGGAAAGCTGTCTTCA | 59.8 | 0.00 |
| mm4_exon_Prkab2/Gm17651_chr3_97671823_R | GTCTCGTGGGCTCGGATCAGCACCCACCCCTCTAT | 60.0 | 0.00 |
| mm4_exon_Ntn5_chr7_45693195_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_exon_Ntn5_chr7_45693195_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_exon_Gm26591_chr6_120035716_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_exon_Gm26591_chr6_120035716_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm3_intron_Frk_chr10_34593753_F | TCGTCGGCAGCGTCGCTACTTGTGGGAGGCATGT | 60.0 | 0.00 |
| mm3_intron_Frk_chr10_34593753_R | GTCTCGTGGGCTCGGCCCTAGCCACTTACGGGTCT | 60.6 | 0.00 |
| mm3_intron_Dscr3_chr16_94511605_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm3_intron_Dscr3_chr16_94511605_R | Primer3: not found at this Tm | N.d. | 0.00 |
These only list off-targets that have primers in the table above. Primers underlined, off-targets in bold.
| ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52313943 |
AATTTGTCCGAAGCCGTGAGCAGCCCGCTGCCTGAACCGCCAGAGAAGATGGTGCCCCACGGTTGCCTGAGCCCGCGAGC CGGCCCTCCGACTTCCCGGGAGCGTGGCGGGGGAGGCCAGGAGGAGGAG |
| mm4_intergenic_Calm1/Gm26723|Ttc7b_chr12_100221581 |
GTTTCCAGGAGGAGCTGTCCAACAAGGCTCTCTGGTGGTCCAAGCAGTGGGGCCTCGAGCATCCAAGACAGGCATGGCAG AAAGCAGTAGACTGTAGGTGGGAGCAGCCTGTTCTCCACTCCTG |
| mm4_exon_6530409C15Rik/Zfp800_chr6_28260539 |
AAGAAGGGAAAGGAGCTGCCCGACCTTGTGGAAATGATTGACGGTGACACTTGTGGCACTTTCTCTGGGGGCTCAAGCAA TGGAGATCTCCAGTTTGTACAGGCTTTTGGCATTACGCGAGT |
| mm3_intergenic_Gm16565/Cd247|Gm23402_chr1_165801935 |
CCGGGGTTGCCCTTTAGAAAAACATCACCAAGCACATTCTGTAGTCTCAGAATGAGTTCGCTGGTGATTCAGGCAGTGGG GCTTGATCTGAGTTTCAGACATCTCTATTCATTTCACGGCCTCTGTTTCCC |
| mm4_intron_Nck2_chr1_43519691 |
GTGTGTAAGCAGCAGACCCTACACCTTCTCCTGCTGATCAGGCAGAGGAATGTTTGGTCCACAGAAGCTCACTTCATATG CAGTAGCCTCCCCAGCAAAGGAGACATGT |
| mm4_intergenic_Gm26619/Gm10260|Arhgef28_chr13_97874486 |
TCCAGACTAGATGGGGCAGCTCCACTGCCTGAATAGCCAAGGAAGCCTACCCCCTCATTCTTCTCCTTTGTGGAAGCTAG ACTATTTTAGCTGAGCCGTGGTCAAG |
| mm4_intergenic_Chsy1/Gm26827|Lrrk1_chr7_66197313 |
TTTTGCTTCCTCTGGGGTGGGAGGGTGTGTGGGGACCAGAGCTGAGAAAAGCCTGTGCCTGAACCTTCAGAAAAGAGAGT CTAAACAGAAGCCACTTCCAGGAAGGGGTAGAGCTGGGGTGTCAGAGAT |
| mm4_intron_Oscar_chr7_3615852 |
CAGCACAGTAGACTTGCCCATTTACCATGAGAACTGAATACATTCTTTGACCCCTAGATATCCCTTACTCTCCTCTGCCT GGAGATCCAGAGAAACCTATGCTCACCCCCGACCCAGCAATCTGTCTT |
| mm4_intron_Pard3b_chr1_62405286 |
AGCAGAGCACTTCAGGAAGGGTACGGTTACAATGCTGTGTTCTCTGCCAGCTCAGGCACAGGAAGCCAGCAGCAGCTAAC AACGTAACATTCATTATGCTATGAGATTATCTTAAATCCAGATGGGGCCGC |
| mm3_intergenic_Adcy5|Gm25179_chr16_35223477 |
TGGCTCTTTGTCTGCCTCTGTACAGAGCAGAAGTCCCTCCCATGCCTGAAACTCCAGAGAAGGCAGCTTCACCTGGGAAT GA |
| mm4_intron_Sipa1l1_chr12_82332792 | CAGATTGGGAGCTGGTGGTGGCTTCTCTGCCTCTTCAGACAGCGGCCCTCCTTGCTGGTTGTCTGCTTTGCTGGAGC |
| mm4_intron_Slco3a1_chr7_74298122 |
TCCGTGTCTAGTACCCAGCTTTTGCTGAGGGGATGCATATGTGGGTGGCAAGCTTGGCAGATGTTCTCTGGCTTACCAGG CAGGGGAATATATACCTATGGGTTTGTGGTAAAAGGCTCTGAGGG |
| mm4_intergenic_Dcc|Gm25509_chr18_72549905 |
TCCCATTCTTTTCCTTCAGGACTTGTTTTTTCTTTTTTAATCTAGTCCCCAGTGCCTATACCACCAGAGAAAATAACACT CTGTCCTCTGGGTATAGCTGA |
| mm4_intergenic_Hist1h2bc|Hist1h1t_chr13_23695191 |
TAGCCCTGAGACTGGCTGATTTGACCACGTAGGTGCCATGGGTAAGATGTTTATAACCGCATCCGAAGCCCTTCTCTTGA GATTCAGGCGGGGGAATAGCGAGCCTCTCACATGC |
| mm4_intergenic_Gm7488|Tbl1xr1_chr3_21574362 |
GCAGAAGCAAACTTATTTGGAGTTATTTCCTCTGCCTGAACCTCCCTGGAAGGAAGATGATTAGATATAGCCTCGTGTCA CACTGGACAA |
| mm4_intron_Unc5b_chr10_60787797 |
GACACAGAACACTGCCTCCATCTGGCCCTAGATCCTGTGGGGGTTCAGGCTGTGGTCCCCCCTCAGAGGCTGTTTCAATG CTCGTTACTGATGACAAATGGGGGGCTGGCCAGCTTGGAGGACACCAAGTTGCTC |
| mm4_intron_Mmel1_chr4_154870900 |
GGAGGACAGCAGAGGTCTTGTGAGGATGTGAAGGACCAGACAGCCACTGACTGAGCATCCAGAGAAATGCAGGGCATGTG AACACCAAGAGACAGGCAGCAGCTGACTGACAGCCAGGAAGCGGGGAGTGAGCATCTAAA |
| mm3_intergenic_Gm23351|A530084C06Rik/Foxq1_chr13_31504551 |
CATACCCGAGAGCCATGGTCACTGCCACTGCCTGCACAGACAGAGAATCAGTACAGGGTATACTGATTGCCCCTGGTGCT TGTACCTCTGTGAAGCTGAGAAACCATGATCCCAGGCCAG |
| mm4_intron_Ulk4_chr9_121030314 |
CCGATGGGTTGTTGGAGAGGATTCTTTTGAGTGGGATTAGACCCGACATGGGCTATTCTCTGGGGAAGCAGGCAGTGGGA TCCAGACCAAACTGTGGGAAGGTCCA |
| mm4_intron_Cela1_chr15_100675272 |
TATCCAGCCTGGGGTTTCCTGTCCAACCCAGCCAGCGCCTGGATCGCCAGAGAATTTCAACCCATCATTTTAAAGGACTC ACATTATTCATCCAGGAAATGTAAGCAGAGACTCTGGTGAAGACGGTGG |
| mm4_intron_Als2_chr1_59198981 |
AGCTAAAACAAGAGCCCGGATGATCTGGGGGTGCAGGCAGCGGGAGGTATCAACTTACCTAAACTGAGAAGAGGCCTAAG TATCTGTGATTTGATTTCGCTCAGTTTTGAGTAAAACCGTCTTTCTGTAGAAGCCA |
| mm4_intron_Scn11a_chr9_119825373 |
AAAGGGGCTTGATCTCAGCCTGACACAAAGACACAAAGCTCTCTAAATAACAAGCCCCAGCCTGAAACGACAGAGGATGG GGTAGCCCCAGCCTGGATGGCTTACCTGTCTTCCTGC |
| mm4_intergenic_Gm23217|Gm24390_chr15_72477298 |
TTCCCAGTCTGAGGCCCTAAGTCACATTTATCAGGGCTTAAAAAGGCAAAACCCATAACTAACTGCATTTCTCATGCGGT CCAGTCAGGGGTAAACATACATCCTGATGTATTTCCTGCTTACGTGTGATCAAGC |
| mm4_exon_Traf5_chr1_191997663 |
TGGGGTCAGCTTTGAAGGTCTCCACAATATGGTTCTTCTTGCCGCTCTGGTCCAAAAGCATCAGGGTCACCCTCTGCCTG AACGGCCACTGCAGCAGCGAGTCAAACTCACCGCGCATCACCACAAAGTAC |
| mm4_intergenic_4930435H24Rik|Qdpr_chr5_45239452 |
GCACGGTAGGTCGTAATGGATTTGTGCCTCTGCCAGACCTTCCAGAGAACTAGTTCACGTAGTAATGTGACATGACTTAG AGAGTCATAATCACTATGACAACATAAAGGGAATCAGTTAAAGGCCA |
| mm4_intron_Armc2_chr10_41935926 |
TTGTTGCCCCTGTGGAAGTCTATTCTGAAAACTTCTGTAGAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGAGAAATAC CACATACAAACTCAGCTCACTTCACCCAGG |
| mm4_intergenic_Ube3b|Mmab_chr5_114430909 |
TCTGCTCAGCCTGGACAAAGTGCCACCCTTCAGGAGGGTGCCACGCCCCCTGCCAGAACCCCCAAAGACAAATTTATCCC TCTTGGTGATCAAGACAGGACCAGCTTGG |
| mm4_intergenic_4930584F24Rik|Dpp6_chr5_26710367 |
GAGCTGAGGTCATGCTGTGTAGAAGACTGTGTGAGGCCCATAATGATGAGTTCACTGGTGGTTCTGGTAGAGGCGTCATA GGGAGGGAGGAATATCAGCCTCTGCGGTGTG |
| mm4_exon_Kif13b_chr14_64772900 |
CACATCAGGGCAGTTCTCGAAAGGGAGCCTTCACATGGAGAAGAAAGTGAAGTGCTGCCTTACCTTCTCTGCAGGTGAAG GCAGAGGCCTCCTGGGACTCTGCAGTGCACAGCTGTCCTCAACTC |
| mm4_intron_Aqr_chr2_114146809 |
TGAGGTTAGCAAAGGGCCAGCCCGCAGCCCGCAGCGCCAGAGAAGAAAGAAAAGCCCACAGCGAAGCCTAGGAGACAATG CTGTTCTTTCCATGTCTTACCTGAGAGGCCCAAAAAACTTGACCAAGGAATCTCGGGC |
| mm3_intergenic_Gm13851|9430029A11Rik_chr6_32412347 |
GCCTGGGAGACACCTGAAAATCTTCTGTGGCACTTCAGGCAGGGAACATAAATCATTCCCCTCTCACTTCTTCTTCCCAA TCATGCTTTCTTGCTGCATCAGCTGAATCTTCCAATTGGCCCTGGTG |
| mm4_intron_Prr5l_chr2_101753931 | TCACCCACCCTCCCTATCAGGCTCCTTTGTGCCCCACGCTGAACCTCCAGAGAATGCATGGAAGTCCCCTCCTTC |
| mm4_exon_Fbrsl1_chr5_110379580 | GCCTTGGCCTCTGACTTCTCACCTCTCCCAGAACCTGCAGAGAAAGGGCAGGTGTGTTGTAAGACCTGGCGCTT |
| mm4_intron_Tbc1d30_chr10_121293339 |
TAGAGGCACAGGTCCACCTTCTCCTATGGACTGTACAGCACAGGTACACCTTCTCTGGCTGTAGAGGCACAGGTCCACCT TCTCCTATGGACTGTACAGGCACAGG |
| mm4_intron_Slc23a2_chr2_132087141 |
CACCTGGAGTCACTTGCACTTTCTACAGATGGGTCAATAGGTATCTGTGGGGCTGTAGAACTTTCCCTCCTTCATAGGTC ATGACTGGATACCACCCATGCCTCAACAGCCACAGAATCCCACATCCCAGGTCCAGCTTG |
| mm4_intron_Fmn2_chr1_174751680 |
GTGAGAACAGTGGCTCCCTCTGCAGGTTCTGTGGCTTTTGAGGCAGGGGGATGAAGATGAAATGTTCTTGCTTTCAGCTT TTATGCACTCTAAATAGAAAGATTTCAGTACTGTCTGCTCCCGA |
| mm4_intergenic_Kcnv1|Gm5472_chr15_45361306 |
TGGTGCTGCACAATTGTTGGTGCTGCATGACAGTAATTGATCATGGTGATGTGCAATTATAACATCTATTGNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNTTTAGTGATTTCAGGGGAATGTGGAGGAAAGT |
| mm4_intergenic_Gm12764|Gm12766_chr7_34405116 |
TTTAAGCTCACGGGACCTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGGAGGGGCTTAGTGACA |
| mm4_exon_Prkab2/Gm17651_chr3_97671823 |
GGTGGGGAAAGCTGTCTTCAGTCTCTCACTCAGGCTGATGCTTCTCTGACGGGTGAGGCTGTGGTGATAGAGGGGTGGGT GCTGAT |
| mm3_intron_Frk_chr10_34593753 |
GCTACTTGTGGGAGGCATGTAAGTTTTGTGGGATTCTCTGGTGGTTGAGGCTGAGGTTGTGACAGAAGTGAGTGTTGCTA ACTGGGGGAAGTTCACTAGGCTTTCTTAAGACCCGTAAGTGGCTAGGG |