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Contents:In the table below, Illumina Nextera Handle sequences have been added and highlighted in bold. Primers for the on-target have been added for convenience. The table below is sorted by the CFD off-target score. Sites with very low CFD scores < 0.02 are unlikely to be cleaved, see our study Haeussler et al. 2016, Figure 2.
In the protocol by Matthew Canver, Harvard, two PCRs are run: one PCR to amplify the potential off-target, then a second PCR to extend the handles with Illumina barcodes. Please click here to download the protocol. Alternatively, you can have a look at Fu et al, 2014.
If a primer was not found, the reason is usually that the region around the off-target is too repetitive. To avoid unspecific primers, all repeats are masked for the primer design (not for off-target search). If you think that we should change the parameters here or should use different primer3 settings, please let us know.
| Name | Primer Sequence | Tm | CFD Score |
|---|---|---|---|
| ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52314222_F | TCGTCGGCAGCGTCACCTAGTAGCTCAGACACCGA | 59.7 | 1.00 |
| ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52314222_R | GTCTCGTGGGCTCGGACCTTTGCTGTGCTGGTACT | 59.5 | 1.00 |
| mm4_exon_Gm26653_chr6_136175262_F | TCGTCGGCAGCGTCTTCTCCTGTTGCCCAAACCT | 59.4 | 0.27 |
| mm4_exon_Gm26653_chr6_136175262_R | GTCTCGTGGGCTCGGCGACCTGGAATTAGACTCCCA | 58.8 | 0.27 |
| mm4_intron_Poc1a_chr9_106302710_F | Primer3: not found at this Tm | N.d. | 0.24 |
| mm4_intron_Poc1a_chr9_106302710_R | Primer3: not found at this Tm | N.d. | 0.24 |
| mm4_intron_Odf2_chr2_29908592_F | Primer3: not found at this Tm | N.d. | 0.20 |
| mm4_intron_Odf2_chr2_29908592_R | Primer3: not found at this Tm | N.d. | 0.20 |
| mm4_intergenic_Gm6984|Htr2a_chr14_74229284_F | TCGTCGGCAGCGTCTCTACCACTGAATGCCTGCC | 59.7 | 0.19 |
| mm4_intergenic_Gm6984|Htr2a_chr14_74229284_R | GTCTCGTGGGCTCGGTCATCCCCTTGCTGACAACC | 59.9 | 0.19 |
| mm3_intron_Atp11b_chr3_35833251_F | Primer3: not found at this Tm | N.d. | 0.18 |
| mm3_intron_Atp11b_chr3_35833251_R | Primer3: not found at this Tm | N.d. | 0.18 |
| mm4_exon_Gm26883/Tshz2_chr2_169884478_F | TCGTCGGCAGCGTCTGGTCACACCAGCCAAGAAG | 60.1 | 0.16 |
| mm4_exon_Gm26883/Tshz2_chr2_169884478_R | GTCTCGTGGGCTCGGCAGCTGCAGGTTAGCACTCT | 60.0 | 0.16 |
| mm4_intergenic_Gm23064|Gm12718_chr4_103481600_F | Primer3: not found at this Tm | N.d. | 0.16 |
| mm4_intergenic_Gm23064|Gm12718_chr4_103481600_R | Primer3: not found at this Tm | N.d. | 0.16 |
| mm4_exon_Bik_chr15_83526852_F | TCGTCGGCAGCGTCTCACAATAACCCGCCTCCAG | 59.7 | 0.15 |
| mm4_exon_Bik_chr15_83526852_R | GTCTCGTGGGCTCGGCTTACCTGCCAAGCTGAGCT | 60.0 | 0.15 |
| mm4_intergenic_Atg5|Prdm1_chr10_44417788_F | TCGTCGGCAGCGTCTGCACCACATCTTATCCCCA | 58.7 | 0.15 |
| mm4_intergenic_Atg5|Prdm1_chr10_44417788_R | GTCTCGTGGGCTCGGGGGATCTGCGGATGGAAACT | 59.8 | 0.15 |
| mm4_intergenic_Gm13819|Gm13821_chr5_114834349_F | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_intergenic_Gm13819|Gm13821_chr5_114834349_R | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_intron_Lpp_chr16_24953104_F | TCGTCGGCAGCGTCGTGACAGTGTGAGGGGATGG | 60.0 | 0.12 |
| mm4_intron_Lpp_chr16_24953104_R | GTCTCGTGGGCTCGGATCAGGAGACATGCATGGGG | 59.5 | 0.12 |
| mm4_exon_Ssh1_chr5_113942318_F | Primer3: not found at this Tm | N.d. | 0.11 |
| mm4_exon_Ssh1_chr5_113942318_R | Primer3: not found at this Tm | N.d. | 0.11 |
| mm4_intergenic_Id2|Gm22527_chr12_25105310_F | Primer3: not found at this Tm | N.d. | 0.11 |
| mm4_intergenic_Id2|Gm22527_chr12_25105310_R | Primer3: not found at this Tm | N.d. | 0.11 |
| mm4_intron_Rbfox1_chr16_7149507_F | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intron_Rbfox1_chr16_7149507_R | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intergenic_AI846148|Rab11b-ps2_chr19_7392956_F | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intergenic_AI846148|Rab11b-ps2_chr19_7392956_R | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intron_Kirrel_chr3_87131003_F | TCGTCGGCAGCGTCCATCCACGGTCTCAGTTCCC | 60.1 | 0.08 |
| mm4_intron_Kirrel_chr3_87131003_R | GTCTCGTGGGCTCGGCTCCGCTTTCCTGGGTCATT | 60.0 | 0.08 |
| mm4_exon_Mmab_chr5_114434870_F | TCGTCGGCAGCGTCCCCAGGACACACATAACCCC | 60.0 | 0.07 |
| mm4_exon_Mmab_chr5_114434870_R | GTCTCGTGGGCTCGGACAGCCATGCTTCTGGTCTC | 60.0 | 0.07 |
| mm4_intron_Tnrc6c_chr11_117759841_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intron_Tnrc6c_chr11_117759841_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intron_Wdr4_chr17_31505512_F | TCGTCGGCAGCGTCACTCCAGGAGCTGAGGTAGG | 60.0 | 0.07 |
| mm4_intron_Wdr4_chr17_31505512_R | GTCTCGTGGGCTCGGGGCTCTTGGCTGTAAACCCT | 59.9 | 0.07 |
| mm4_intron_Tbc1d25_chrX_8174926_F | TCGTCGGCAGCGTCAACCTTTGCCTTAGAGCGCT | 59.9 | 0.06 |
| mm4_intron_Tbc1d25_chrX_8174926_R | GTCTCGTGGGCTCGGGGGCACATAGGACAGCTTGT | 60.0 | 0.06 |
| mm4_intron_Bcl2l11_chr2_128152610_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intron_Bcl2l11_chr2_128152610_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_Rbfox1|Gm23476_chr16_7695948_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Rbfox1|Gm23476_chr16_7695948_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Chst11|1700025N21Rik_chr10_83125513_F | TCGTCGGCAGCGTCTGGACTTTTCTTCAGGCCCC | 59.8 | 0.03 |
| mm4_intergenic_Chst11|1700025N21Rik_chr10_83125513_R | GTCTCGTGGGCTCGGACAAGGCTTTAGCAGAACTCAGA | 59.9 | 0.03 |
| mm4_exon_Ltbp4_chr7_27319656_F | TCGTCGGCAGCGTCTCCACGAACACTCACCTTGG | 59.8 | 0.03 |
| mm4_exon_Ltbp4_chr7_27319656_R | GTCTCGTGGGCTCGGCGAGAATACGGCTCTGCGAT | 60.0 | 0.03 |
| mm4_intron_Vapb_chr2_173768939_F | TCGTCGGCAGCGTCCAGTGCTGCCTTCAAAGCTG | 60.0 | 0.03 |
| mm4_intron_Vapb_chr2_173768939_R | GTCTCGTGGGCTCGGCCTTCCTGGCCCTCTCTAGA | 59.7 | 0.03 |
| mm4_intron_Rtn4rl2_chr2_84875423_F | TCGTCGGCAGCGTCTCCTTCTGCTTAGCCCCTCT | 59.9 | 0.03 |
| mm4_intron_Rtn4rl2_chr2_84875423_R | GTCTCGTGGGCTCGGTGCTTAGCCTGATACCACCC | 59.1 | 0.03 |
| mm4_intron_Gabpb1_chr2_126674726_F | TCGTCGGCAGCGTCCAACAGCACACTCAACACCG | 59.9 | 0.02 |
| mm4_intron_Gabpb1_chr2_126674726_R | GTCTCGTGGGCTCGGCCATTCTCAGTGGCCGAGAC | 60.4 | 0.02 |
| mm4_intergenic_Gm22321|Gm1401_chr6_33135066_F | TCGTCGGCAGCGTCACAGAAGGTTTTCATTGCCCC | 59.0 | 0.02 |
| mm4_intergenic_Gm22321|Gm1401_chr6_33135066_R | GTCTCGTGGGCTCGGTAAATGGACTGGCTGCCCTG | 60.0 | 0.02 |
| mm4_intergenic_Clrn1|Gm22491_chr3_58888307_F | TCGTCGGCAGCGTCGCTCCAGCCTTTGCACATTC | 60.1 | 0.02 |
| mm4_intergenic_Clrn1|Gm22491_chr3_58888307_R | GTCTCGTGGGCTCGGGGACAGACGCATCTCATCGT | 59.8 | 0.02 |
| mm4_intron_Cers6_chr2_69020902_F | TCGTCGGCAGCGTCTGCAGTGTATGAGCTGTGCT | 59.6 | 0.02 |
| mm4_intron_Cers6_chr2_69020902_R | GTCTCGTGGGCTCGGCCCAAAGAGAATAGAGGTTGCC | 58.7 | 0.02 |
| mm4_intergenic_Mirlet7b|Ppara_chr15_85709448_F | TCGTCGGCAGCGTCAGAGGGTGGAGTCAGGATTCA | 59.9 | 0.01 |
| mm4_intergenic_Mirlet7b|Ppara_chr15_85709448_R | GTCTCGTGGGCTCGGACAACCCCAGGAGTCACTTC | 59.2 | 0.01 |
| mm4_intergenic_App|Cyyr1_chr16_85291191_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_App|Cyyr1_chr16_85291191_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_Auh_chr13_52859529_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_Auh_chr13_52859529_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_Ptprn2_chr12_116654682_F | TCGTCGGCAGCGTCAATCTGGACCAATGGGCAGG | 60.0 | 0.01 |
| mm4_intron_Ptprn2_chr12_116654682_R | GTCTCGTGGGCTCGGGTGTCTGACAGGAAGCCCAG | 60.3 | 0.01 |
| mm4_intron_Nucb2_chr7_116539054_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_Nucb2_chr7_116539054_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Gm22169|Gm22962_chr9_68474566_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Gm22169|Gm22962_chr9_68474566_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_n-R5s86|Wdr72_chr9_74067076_F | TCGTCGGCAGCGTCATCTGACCAGCTGTGTGCAG | 60.3 | 0.01 |
| mm4_intergenic_n-R5s86|Wdr72_chr9_74067076_R | GTCTCGTGGGCTCGGGGACCTCCTGTAGCTTCCCT | 60.3 | 0.01 |
| mm4_exon_Sh3tc1_chr5_35699839_F | TCGTCGGCAGCGTCGGAAGAAGTGCTCAGCCAGT | 59.9 | 0.01 |
| mm4_exon_Sh3tc1_chr5_35699839_R | GTCTCGTGGGCTCGGTGAATGAGCGAGTGGCCTAC | 59.8 | 0.01 |
| mm4_intron_2010315B03Rik_chr9_124300107_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_2010315B03Rik_chr9_124300107_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_Lnx2_chr5_147074044_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_Lnx2_chr5_147074044_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_Apaf1_chr10_91036327_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_Apaf1_chr10_91036327_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Gm13420|Gm13547_chr2_29748489_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Gm13420|Gm13547_chr2_29748489_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm2_intron_Fstl4_chr11_52908691_F | TCGTCGGCAGCGTCCCTTGGTGTGACATTCTGCC | 59.1 | 0.01 |
| mm2_intron_Fstl4_chr11_52908691_R | GTCTCGTGGGCTCGGAATAGCCCCACCACTCCTCT | 59.9 | 0.01 |
| mm4_intron_Gm16286/Txnl4a_chr18_80207074_F | TCGTCGGCAGCGTCGAGCAGTGGAGGTTTGGGG | 60.3 | 0.00 |
| mm4_intron_Gm16286/Txnl4a_chr18_80207074_R | GTCTCGTGGGCTCGGGAAAGAGCAGAGGATCGCGA | 59.8 | 0.00 |
| mm4_intron_Cpne2_chr8_94561322_F | TCGTCGGCAGCGTCCAGAGTGAGGCAGGATGCTG | 60.4 | 0.00 |
| mm4_intron_Cpne2_chr8_94561322_R | GTCTCGTGGGCTCGGGAAGCTGGCTGGTGTCCTAG | 60.1 | 0.00 |
| mm4_intron_Gm16984_chr13_34966481_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Gm16984_chr13_34966481_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_exon_Ctsa/Pltp_chr2_164839372_F | TCGTCGGCAGCGTCGAGTCCTCCGCTAATGCTCC | 59.9 | 0.00 |
| mm4_exon_Ctsa/Pltp_chr2_164839372_R | GTCTCGTGGGCTCGGGCTCACTTGGTCCTAGGCTG | 60.1 | 0.00 |
| mm4_intron_Erc2_chr14_28295953_F | TCGTCGGCAGCGTCAAGAATTGCCGCTATGGGCT | 60.1 | 0.00 |
| mm4_intron_Erc2_chr14_28295953_R | GTCTCGTGGGCTCGGTTAGTCACAGGGCACAGCAG | 59.9 | 0.00 |
| mm4_intergenic_Gria1|Gm12242_chr11_57399496_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gria1|Gm12242_chr11_57399496_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_exon_Kctd17_chr15_78435856_F | TCGTCGGCAGCGTCTCATGGTCATGGTCACAGCC | 60.0 | 0.00 |
| mm4_exon_Kctd17_chr15_78435856_R | GTCTCGTGGGCTCGGCTCTGTCTGGAGCCTTGCAA | 59.9 | 0.00 |
| mm4_intron_Dennd2c_chr3_103102883_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Dennd2c_chr3_103102883_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Ddx59|Kif14_chr1_136456159_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Ddx59|Kif14_chr1_136456159_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Smoc2|Thbs2_chr17_14493872_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Smoc2|Thbs2_chr17_14493872_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Spta1_chr1_174222348_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Spta1_chr1_174222348_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Memo1_chr17_74293472_F | TCGTCGGCAGCGTCAGGAGCCAGAGACCTGACTT | 59.8 | 0.00 |
| mm4_intron_Memo1_chr17_74293472_R | GTCTCGTGGGCTCGGATGTCAGTTCTTGTGCCAGT | 57.3 | 0.00 |
| mm4_exon_Fam171b_chr2_83812629_F | TCGTCGGCAGCGTCCCTCCTCCTCCTGCCGAG | 60.8 | 0.00 |
| mm4_exon_Fam171b_chr2_83812629_R | GTCTCGTGGGCTCGGACGGAGGCATATTGCGGG | 59.8 | 0.00 |
| mm4_intron_Zmiz1_chr14_25630781_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Zmiz1_chr14_25630781_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Tox2_chr2_163276769_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Tox2_chr2_163276769_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Slc12a3_chr8_94352924_F | TCGTCGGCAGCGTCGCTAAGGTCCAGCAAGGGTT | 59.9 | 0.00 |
| mm4_intron_Slc12a3_chr8_94352924_R | GTCTCGTGGGCTCGGGGATGAGGAGGGTGAGACCT | 60.0 | 0.00 |
| mm4_intron_Shank2_chr7_144267386_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Shank2_chr7_144267386_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Sh3bp5_chr14_31428397_F | TCGTCGGCAGCGTCTCACACAGGCCACATCTGTC | 59.9 | 0.00 |
| mm4_intron_Sh3bp5_chr14_31428397_R | GTCTCGTGGGCTCGGAGCTCGGGTCATCTGACGTA | 60.3 | 0.00 |
| mm4_intron_Ptprf_chr4_118221965_F | TCGTCGGCAGCGTCGTTCAACAGCTAGCCATGTGC | 60.1 | 0.00 |
| mm4_intron_Ptprf_chr4_118221965_R | GTCTCGTGGGCTCGGTGGCTCCTGGTCCCATCTTT | 60.8 | 0.00 |
| mm4_intron_Plin5_chr17_56116943_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Plin5_chr17_56116943_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Morn1_chr4_155095539_F | TCGTCGGCAGCGTCCAGCAGGGAGGCCTTGAATG | 61.0 | 0.00 |
| mm4_intron_Morn1_chr4_155095539_R | GTCTCGTGGGCTCGGCTTCAGGAGGAGAAGCCACG | 60.1 | 0.00 |
| mm4_intron_Il23r_chr6_67424962_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Il23r_chr6_67424962_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Hs2st1_chr3_144440791_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Hs2st1_chr3_144440791_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Gria1_chr11_57017165_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Gria1_chr11_57017165_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Fam178b_chr1_36644087_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Fam178b_chr1_36644087_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Ccdc149_chr5_52426972_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Ccdc149_chr5_52426972_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Apba1_chr19_23920593_F | TCGTCGGCAGCGTCGGCTCCATCACAAGTCCTGT | 59.6 | 0.00 |
| mm4_intron_Apba1_chr19_23920593_R | GTCTCGTGGGCTCGGAGAAGAGGGGTGAGGGAACA | 59.8 | 0.00 |
| mm4_intron_Amph_chr13_19007383_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Amph_chr13_19007383_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Uaca|Gm10655_chr9_61355417_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Uaca|Gm10655_chr9_61355417_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Thsd4|Gm23668_chr9_60010978_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Thsd4|Gm23668_chr9_60010978_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Slc7a15|Pum2_chr12_8657922_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Slc7a15|Pum2_chr12_8657922_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Rcn1|4930527A07Rik_chr2_105444836_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Rcn1|4930527A07Rik_chr2_105444836_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Psd2|Nrg2_chr18_36017545_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Psd2|Nrg2_chr18_36017545_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Poc1a|Dusp7_chr9_106353229_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Poc1a|Dusp7_chr9_106353229_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Mzt2|Cebpd_chr16_15865001_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Mzt2|Cebpd_chr16_15865001_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Ipmk|Gm22937_chr10_71517645_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Ipmk|Gm22937_chr10_71517645_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm4825|Wnt7b_chr15_85513446_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm4825|Wnt7b_chr15_85513446_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm26177|Gm25602_chr10_54708602_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm26177|Gm25602_chr10_54708602_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm24434|Arl4a_chr12_39789862_F | TCGTCGGCAGCGTCGCATAGCAGACAGGGAAGGG | 60.1 | 0.00 |
| mm4_intergenic_Gm24434|Arl4a_chr12_39789862_R | GTCTCGTGGGCTCGGTGGGCAGACACATCAGTTCT | 58.9 | 0.00 |
| mm4_intergenic_Gm22689|Gm9742_chr13_7736408_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm22689|Gm9742_chr13_7736408_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm15387|Bai1_chr15_74128731_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm15387|Bai1_chr15_74128731_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm12152|Pwwp2a_chr11_43677856_F | TCGTCGGCAGCGTCTCCTTGAAGAGGTGCTGCTG | 59.9 | 0.00 |
| mm4_intergenic_Gm12152|Pwwp2a_chr11_43677856_R | GTCTCGTGGGCTCGGGCTGCCAGACATCCAGGAAT | 60.1 | 0.00 |
| mm4_intergenic_Bambi|Gm23643_chr18_3761228_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Bambi|Gm23643_chr18_3761228_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Asic2|Gm11416_chr11_81019697_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Asic2|Gm11416_chr11_81019697_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Asb1|Twist2_chr1_91599841_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Asb1|Twist2_chr1_91599841_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_exon_Papolg_chr11_23867968_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_exon_Papolg_chr11_23867968_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_exon_Dcakd_chr11_102994077_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_exon_Dcakd_chr11_102994077_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm3_intergenic_Notch1|Gm13358_chr2_26540677_F | TCGTCGGCAGCGTCAGGAAGGTGCCTACTGCCTA | 59.9 | 0.00 |
| mm3_intergenic_Notch1|Gm13358_chr2_26540677_R | GTCTCGTGGGCTCGGAGAAGGTGCAGATGTGAGCC | 60.0 | 0.00 |
| mm3_intergenic_Jazf1|Gm15572_chr6_52833300_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm3_intergenic_Jazf1|Gm15572_chr6_52833300_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm3_exon_Pygm_chr19_6392825_F | TCGTCGGCAGCGTCGGCCCTGCACCTTGAACATA | 60.3 | 0.00 |
| mm3_exon_Pygm_chr19_6392825_R | GTCTCGTGGGCTCGGCTTAGCCATGTGGTACCCGG | 60.1 | 0.00 |
These only list off-targets that have primers in the table above. Primers underlined, off-targets in bold.
| ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52314222 |
ACCTAGTAGCTCAGACACCGAATCGGATTTCTATGAAGAAATCGAGGTGAGCTGCACCCCAGACTGCGCCACCGGGAACG CCGAGTACCAGCACAGCAAAGGT |
| mm4_exon_Gm26653_chr6_136175262 |
TTCTCCTGTTGCCCAAACCTAACATTTCCATTTCAGTCTATCCACTTTCCCTAGCAGAGAGCAAAGAAGGAACACACATC TCCTCTGCCCGTGGCGCAGTCCACGGTGCTCTCCTTTGGGAGTCTAATTCCAGGTCG |
| mm4_intergenic_Gm6984|Htr2a_chr14_74229284 |
TCTACCACTGAATGCCTGCCTTCAGGGAACTTTGACATATACTTCCTGCTTGTTCATTCATTCATTTCCCCTGCACCCCA AACAGCACCATTGGAAAAGCCATGGTTGTCAGCAAGGGGATGA |
| mm4_exon_Gm26883/Tshz2_chr2_169884478 |
TGGTCACACCAGCCAAGAAGCGTGTTTTCGATGTCAACAGGCCTTGCTCCCCTGACTCCACCACAGGGTCCCTTGCAGAT TCCTTTTCTTCCCAGAAGAGTGCTAACCTGCAGCTG |
| mm4_exon_Bik_chr15_83526852 |
TCACAATAACCCGCCTCCAGCCAGCGCCCCCGACTCCGCCACCGGCGTCCCAGCCGAGAGGGTGTTCGGGCAGTTCGCCC GCCGCTACGCCAGCTCAGCTTGGCAGGTAAG |
| mm4_intergenic_Atg5|Prdm1_chr10_44417788 |
TGCACCACATCTTATCCCCATGTTTCTTTGTCACTCATCAATACTGTAGTGTTGTCCACCTCAGACTTCACCACAGGAAG CAATGGCCAAACTCGAAGACAGCAAAGTGTGGGAAGAAGTTTCCATCCGCAGATCCC |
| mm4_intron_Lpp_chr16_24953104 |
GTGACAGTGTGAGGGGATGGGCAAAGGGGCTGAATGTAAGCATCTGACCCCGTGGCGCAGTGTGGGTAGTAAGAAGTCAT CTGGAAGTGTAGACAACCATACCCCATGCATGTCTCCTGAT |
| mm4_intron_Kirrel_chr3_87131003 |
CATCCACGGTCTCAGTTCCCTGTTGATGTCCAACCCTGCATGCTCCCCAGGCCTCGCCACGGGCCACCTTTTCTGCTCTG CTTCCCCTTCTCCTCTGAGCAATGACCCAGGAAAGCGGAG |
| mm4_exon_Mmab_chr5_114434870 |
CCCAGGACACACATAACCCCACAGCTAGGTGTTGTGAGGTCACTTCCTGTCTTGCTAGGCAGCTCCTGGGGCGCAGCCTG GTCTGCACTAACACCATCTGAGCCAGGATGCAGTAGAGACCAGAAGCATGGCTGT |
| mm4_intron_Wdr4_chr17_31505512 |
ACTCCAGGAGCTGAGGTAGGCAGGTGCCCCTCTGAACCCCATACTACGCGACGGGAGGTATTAGACATATCCCTAATCTT CTGGTCTCAGGGTTTACAGCCAAGAGCC |
| mm4_intron_Tbc1d25_chrX_8174926 |
AACCTTTGCCTTAGAGCGCTAACACCCCAAAAGTGATCTCTGGCACTCCAGTCTGAGCCACCGGTCACTGCTTGCAGGTA TCACAACGGGTTCCTTCCTCAGAGTGTGTTATATGTCACAAGCTGTCCTATGTGCCC |
| mm4_intergenic_Chst11|1700025N21Rik_chr10_83125513 |
TGGACTTTTCTTCAGGCCCCTTAGTGGCCAAGTTCTCAGTTCATCTCCTAGTTGAAAGCTGCCCACCACACTGCGCCATT GGATAGGTTTCTGAGTTCTGCTAAAGCCTTGT |
| mm4_exon_Ltbp4_chr7_27319656 |
TCCACGAACACTCACCTTGGCACCCGCCCCCTGGTGTAGGCTGATAGCCAGGGTCGCAGGCTGGTGTGCAGCGGTAGGAG CCCGGGGTGTTCTCACAGCGCTGGGCACCACAAATCGCAGAGCCGTATTCTCG |
| mm4_intron_Vapb_chr2_173768939 |
CAGTGCTGCCTTCAAAGCTGCCCCTGTGGCTCAGGCATGGGTGCAGATAGTTCCTTTCTCTTTAGGTTGCCTATAGAAAG GGCCCATAGGCACTAGTCCAGTTGATTCCCTCTAGAGAGGGCCAGGAAGG |
| mm4_intron_Rtn4rl2_chr2_84875423 |
TCCTTCTGCTTAGCCCCTCTCTTGGGGTTTCTCTTCAGCAATAGGAGTGTCAGCAATAGGAGGTCATTCTCAGGGAAGTA CTTCCAGAGGCTCAGTGTGGGGTACAGGAGGGTGGTATCAGGCTAAGCA |
| mm4_intron_Gabpb1_chr2_126674726 |
CAACAGCACACTCAACACCGATCTGTGCCCAGGCAAGACATGACCGACGAAACTCGCGGCTTCTTTCCGCACCCGCGCTC GCGTGGGTTCCGGTGGCGAAGTCTCCGGGGCAAAAGTCTCGGCCACTGAGAATGG |
| mm4_intergenic_Gm22321|Gm1401_chr6_33135066 |
ACAGAAGGTTTTCATTGCCCCTTAAAAATAAGCTGCANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNCTGAGGCTGTTCCAGGGCAGCCAGTCCATTTA |
| mm4_intergenic_Clrn1|Gm22491_chr3_58888307 |
GCTCCAGCCTTTGCACATTCCTTCTGTACCATGTGCATGGGCTCCCCTGACTGTGCCACAGGTACGATGAGATGCGTCTG TCC |
| mm4_intron_Cers6_chr2_69020902 | TGCAGTGTATGAGCTGTGCTGGTTGCAGTGCGCCAGTGGCACAGTGTGGTGTTCAGGCAACCTCTATTCTCTTTGGG |
| mm4_intergenic_Mirlet7b|Ppara_chr15_85709448 |
AGAGGGTGGAGTCAGGATTCAAGGGCACCCCAGATTGTGCCTCAGGGAAAGTACCCAGGAAGGTCCTGGTCCCTGGCCGG CCTTCTGCTGCCACCTGCTGGCTAATCTAAGAAGTGACTCCTGGGGTTGT |
| mm4_intron_Ptprn2_chr12_116654682 |
AATCTGGACCAATGGGCAGGAGACTGCAAGAGAACCCAGTGGCCCTGTCTGAGCTGCAGTGAATTTACATAAGCGGTCCC CAGCTTCTTATGTCCTTTTCTGGGCATCTGCTGGGCTTCCTGTCAGACAC |
| mm4_intergenic_n-R5s86|Wdr72_chr9_74067076 |
ATCTGACCAGCTGTGTGCAGGGGAAGGAGGCAGAGTGCCTGCCATGGAGCCCAGACTGGACCACAGGCGCCCAAGGGAAG CTACAGGAGGTCC |
| mm4_exon_Sh3tc1_chr5_35699839 |
GGAAGAAGTGCTCAGCCAGTTCCCCGTGGCCCAGCCGGTGGTGCAGGGTGGCTAGCCTGTGGTAGGCCACTCGCTCATTC A |
| mm2_intron_Fstl4_chr11_52908691 |
CCTTGGTGTGACATTCTGCCATCTCTACAAAGCCACCTTGCACCCGAGACTGCGCCAGCAGCCGGAATAACGAGTTAAAT GCTAAATATTTCATGGAGAGGAGTGGTGGGGCTATT |
| mm4_intron_Gm16286/Txnl4a_chr18_80207074 |
GAGCAGTGGAGGTTTGGGGTGCGGTGGCCTGCAGGTTTGCAGTGGGGCCCTAGGCAGAGTCTGGGGTGCAGGCCAGGTTC TTGGGGTCCGGATGGGGTCGCGATCCTCTGCTCTTTC |
| mm4_intron_Cpne2_chr8_94561322 |
CAGAGTGAGGCAGGATGCTGTGGAACCCAGACTGGGCCTCTGGGCAAACATAGATGCCATGGGGAGGTGGGCAGGGTTAG CCAGTGGCCAGGCCCAGCAAGCGCTTGTTGCTAGGACACCAGCCAGCTTC |
| mm4_exon_Ctsa/Pltp_chr2_164839372 |
GAGTCCTCCGCTAATGCTCCCGTGGGGCAAGTCCTGTACTAAAACTGCCCAGACTTCTCACAGCCTGGTGCATCCCAGCC TGGGCCTCAGGGCGTCACAGACAGCCTAGGACCAAGTGAGC |
| mm4_intron_Erc2_chr14_28295953 |
AAGAATTGCCGCTATGGGCTAGTTGTCAGTAATACACGTTTATCCGGTCTCTCCATTGTCACAGGTTCCTGTCCTTATTA AAGTATCCCCTCCACCCCAGCCTGGGTCACAGGGCTGCTGTGCCCTGTGACTAA |
| mm4_exon_Kctd17_chr15_78435856 |
TCATGGTCATGGTCACAGCCAACACCATCCATCCCACGCCACCACAGTCCAGTCAGCTCACACCCTTGGCCCAGGCTGGG GAGCAGACAGGCAGGACCATTGCAAGGCTCCAGACAGAG |
| mm4_intron_Memo1_chr17_74293472 |
AGGAGCCAGAGACCTGACTTACACAATTCCTATTTTACCACAGGCCTAGTCTGGGGTGCAGCACTAAATTTCTGTTTCCC CATATACTGGCACAAGAACTGACAT |
| mm4_exon_Fam171b_chr2_83812629 |
CCTCCTCCTCCTGCCGAGGCTGTGCACCGCAGACGGCGCGAGGGGGAGCGAGCGAGCGGGCGCGGCCGGGTTCCGCAGCC CTGGCGCCCGCCGCCTCCCGCAGCCCCGCAATATGCCTCCGT |
| mm4_intron_Slc12a3_chr8_94352924 |
GCTAAGGTCCAGCAAGGGTTCCGCTGTGGCAGTCTGGGGTGGAAAGACACAGAGGGCTCTGTCCTGAGTCCTTTGCTCTT GGCTCCTGGGGATCGTCCCTGCTAGGTCTCACCCTCCTCATCC |
| mm4_intron_Sh3bp5_chr14_31428397 |
TCACACAGGCCACATCTGTCCTGTCAGCACTCTTCCTCTGCACCCCAGACTGCTCTGGAGGGTTACGTCAGATGACCCGA GCT |
| mm4_intron_Ptprf_chr4_118221965 |
GTTCAACAGCTAGCCATGTGCTAGCTGCTGGGGGCTCAGCACATATCCAGTGGGGCACTCTGGGGTAGATGAGGGGCTGT ATAAAGATGGGACCAGGAGCCA |
| mm4_intron_Morn1_chr4_155095539 |
CAGCAGGGAGGCCTTGAATGCCTAAGCAGTGCACACAAGACTGCCACACAGGCTAAAACTGCTCAGGTTGCCGTGGCTTC TCCTCCTGAAG |
| mm4_intron_Apba1_chr19_23920593 |
GGCTCCATCACAAGTCCTGTCCCTAGAATCAATAATAAGATTAAGCATCTTAAGTCTGCAGCTGCTTGNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGGTGTTCCCTCACCCCTCTTCT |
| mm4_intergenic_Gm24434|Arl4a_chr12_39789862 |
GCATAGCAGACAGGGAAGGGAACTTTGGTGCAAGATCCTATCTATTCGTCCTGCACCCCAGACTCCCACATAGGTTTGAT GAGAATTAGAACTGATGTGTCTGCCCA |
| mm4_intergenic_Gm12152|Pwwp2a_chr11_43677856 |
TCCTTGAAGAGGTGCTGCTGGTGTGTGTGTTCTGGAATCTCTGGCTTCCAGGGGATGCTGCTCATGTCCAGTGGAGAAGT CATGGGTGCAGGGTCCTTGAGGCCACCATCATTCCTGGATGTCTGGCAGC |
| mm3_intergenic_Notch1|Gm13358_chr2_26540677 |
AGGAAGGTGCCTACTGCCTAGTCTATACCTATAGCCTAATTCCACTGTACCCCAGGCTGCTCCACTGGCCTAGGCAGCCT TCCTGGCTCACATCTGCACCTTCT |
| mm3_exon_Pygm_chr19_6392825 |
GGCCCTGCACCTTGAACATATGCCTGGGCAGTCACAAGATCAAGGTGTGGGAAGGAGGGTGAAACACTGACCCCCAGACT GCCCCACAGGCTGCACCCGGGTACCACATGGCTAAG |