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Contents:

PCR primers for off-targets of CTCCCCCCCTCTGGAGAGTC CGG

In the table below, Illumina Nextera Handle sequences have been added and highlighted in bold. Primers for the on-target have been added for convenience. The table below is sorted by the CFD off-target score. Sites with very low CFD scores < 0.02 are unlikely to be cleaved, see our study Haeussler et al. 2016, Figure 2.

In the protocol by Matthew Canver, Harvard, two PCRs are run: one PCR to amplify the potential off-target, then a second PCR to extend the handles with Illumina barcodes. Please click here to download the protocol. Alternatively, you can have a look at Fu et al, 2014.

If a primer was not found, the reason is usually that the region around the off-target is too repetitive. To avoid unspecific primers, all repeats are masked for the primer design (not for off-target search). If you think that we should change the parameters here or should use different primer3 settings, please let us know.

Maximum amplicon length:     Primer Tm:

NamePrimer Sequence Tm CFD Score
ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52313819_F Primer3: not found at this Tm N.d. 1.00
ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52313819_R Primer3: not found at this Tm N.d. 1.00
mm4_intron_Cdh13_chr8_119316410_F Primer3: not found at this Tm N.d. 0.57
mm4_intron_Cdh13_chr8_119316410_R Primer3: not found at this Tm N.d. 0.57
mm4_intron_Dgki_chr6_36850349_F TCGTCGGCAGCGTCGGCTCTATCAGGCGTAGAACT 59.0 0.51
mm4_intron_Dgki_chr6_36850349_R GTCTCGTGGGCTCGGGCTGCCCCAATAACTGCCTA 60.1 0.51
mm4_intergenic_Gm24696|Myc_chr15_61984263_F TCGTCGGCAGCGTCTTCTAGGGTCTCTGCAGGCT 59.9 0.49
mm4_intergenic_Gm24696|Myc_chr15_61984263_R GTCTCGTGGGCTCGGTGGGGTGGGGAAAATCAAGG 59.8 0.49
mm3_intron_Efcab6_chr15_83869214_F Primer3: not found at this Tm N.d. 0.46
mm3_intron_Efcab6_chr15_83869214_R Primer3: not found at this Tm N.d. 0.46
mm4_intergenic_4930578I07Rik|Gm24258_chr14_66950799_F Primer3: not found at this Tm N.d. 0.45
mm4_intergenic_4930578I07Rik|Gm24258_chr14_66950799_R Primer3: not found at this Tm N.d. 0.45
mm4_intergenic_Gm26497|Piezo1_chr8_122548801_F TCGTCGGCAGCGTCATGGGACTAGGGCATCACCT 60.0 0.45
mm4_intergenic_Gm26497|Piezo1_chr8_122548801_R GTCTCGTGGGCTCGGTGGGGTGCTTAGCCTCTTTG 59.9 0.45
mm4_intron_Kazn_chr4_142150193_F Primer3: not found at this Tm N.d. 0.44
mm4_intron_Kazn_chr4_142150193_R Primer3: not found at this Tm N.d. 0.44
mm4_intron_Usp2_chr9_44074416_F Primer3: not found at this Tm N.d. 0.44
mm4_intron_Usp2_chr9_44074416_R Primer3: not found at this Tm N.d. 0.44
mm4_intron_Ass1_chr2_31509703_F TCGTCGGCAGCGTCGCGTGTTCATGCATGTGTGT 60.0 0.42
mm4_intron_Ass1_chr2_31509703_R GTCTCGTGGGCTCGGGGCCTGCAGTTGAAAACCTG 59.9 0.42
mm4_exon_Nynrin/Gm26751_chr14_55871428_F TCGTCGGCAGCGTCTGCAACTGGATCTTCAGCGT 59.9 0.41
mm4_exon_Nynrin/Gm26751_chr14_55871428_R GTCTCGTGGGCTCGGGATGAGGTAGTGAAGCCCCG 59.8 0.41
mm3_intergenic_Scaf4|Hunk_chr16_90292832_F Primer3: not found at this Tm N.d. 0.40
mm3_intergenic_Scaf4|Hunk_chr16_90292832_R Primer3: not found at this Tm N.d. 0.40
mm4_intergenic_Gm10293|Gm22798_chr3_52615205_F Primer3: not found at this Tm N.d. 0.39
mm4_intergenic_Gm10293|Gm22798_chr3_52615205_R Primer3: not found at this Tm N.d. 0.39
mm4_intergenic_Cldn4|Cldn3/Wbscr25_chr5_134967530_F TCGTCGGCAGCGTCAAACCGATTCCACCCTGACC 59.9 0.36
mm4_intergenic_Cldn4|Cldn3/Wbscr25_chr5_134967530_R GTCTCGTGGGCTCGGGCCTTGTTCTTGCGTGACAG 60.0 0.36
mm4_intergenic_Gyg|Cpa3_chr3_20191592_F TCGTCGGCAGCGTCTCCAATTCTATTCCCTTCTCAGCA 59.5 0.35
mm4_intergenic_Gyg|Cpa3_chr3_20191592_R GTCTCGTGGGCTCGGGATTCCTGGGCTGCAGATCA 59.8 0.35
mm4_intergenic_Gm20696/Foxp1|Gm20705_chr6_99382634_F Primer3: not found at this Tm N.d. 0.34
mm4_intergenic_Gm20696/Foxp1|Gm20705_chr6_99382634_R Primer3: not found at this Tm N.d. 0.34
mm3_intron_Serpina1f_chr12_103691088_F TCGTCGGCAGCGTCCTTCAGGCAGACTCAGGGTG 60.0 0.34
mm3_intron_Serpina1f_chr12_103691088_R GTCTCGTGGGCTCGGGTGAGCAGTTGTACTCGGCT 60.0 0.34
mm4_intergenic_1700122O11Rik|1700067P10Rik_chr17_48038516_F Primer3: not found at this Tm N.d. 0.33
mm4_intergenic_1700122O11Rik|1700067P10Rik_chr17_48038516_R Primer3: not found at this Tm N.d. 0.33
mm3_intron_Aldh1a2_chr9_71236735_F Primer3: not found at this Tm N.d. 0.32
mm3_intron_Aldh1a2_chr9_71236735_R Primer3: not found at this Tm N.d. 0.32
mm3_intergenic_Slc10a1|Smoc1_chr12_80986904_F Primer3: not found at this Tm N.d. 0.31
mm3_intergenic_Slc10a1|Smoc1_chr12_80986904_R Primer3: not found at this Tm N.d. 0.31
mm4_intron_Chrna4_chr2_181042420_F Primer3: not found at this Tm N.d. 0.30
mm4_intron_Chrna4_chr2_181042420_R Primer3: not found at this Tm N.d. 0.30
mm3_intergenic_Gm24291|Mon1b_chr8_113551665_F TCGTCGGCAGCGTCGCGTTGTTCTGAACCGGAAG 59.7 0.30
mm3_intergenic_Gm24291|Mon1b_chr8_113551665_R GTCTCGTGGGCTCGGCCTCTCCTTCCTCCCTTGGA 59.9 0.30
mm4_intergenic_Gm24231|Gm24222_chr8_15314732_F Primer3: not found at this Tm N.d. 0.28
mm4_intergenic_Gm24231|Gm24222_chr8_15314732_R Primer3: not found at this Tm N.d. 0.28
mm4_intron_Arhgef6_chrX_57249372_F TCGTCGGCAGCGTCTAGGAAGAGGGGAGCATTCC 58.1 0.27
mm4_intron_Arhgef6_chrX_57249372_R GTCTCGTGGGCTCGGGGGCTGATTGCTTTTCTGGC 60.1 0.27
mm4_intergenic_Gm26480|Gm6863_chr12_96819960_F Primer3: not found at this Tm N.d. 0.26
mm4_intergenic_Gm26480|Gm6863_chr12_96819960_R Primer3: not found at this Tm N.d. 0.26
mm3_intron_2310081O03Rik_chr4_56223674_F Primer3: not found at this Tm N.d. 0.25
mm3_intron_2310081O03Rik_chr4_56223674_R Primer3: not found at this Tm N.d. 0.25
mm4_intron_3100003L05Rik_chr7_124659987_F Primer3: not found at this Tm N.d. 0.25
mm4_intron_3100003L05Rik_chr7_124659987_R Primer3: not found at this Tm N.d. 0.25
mm2_intergenic_Gm7168|Dact2_chr17_14172760_F Primer3: not found at this Tm N.d. 0.24
mm2_intergenic_Gm7168|Dact2_chr17_14172760_R Primer3: not found at this Tm N.d. 0.24
mm4_intron_Ccdc6_chr10_70127946_F Primer3: not found at this Tm N.d. 0.24
mm4_intron_Ccdc6_chr10_70127946_R Primer3: not found at this Tm N.d. 0.24
mm4_intergenic_Gm25778|Ppapdc1a_chr7_129129292_F TCGTCGGCAGCGTCCAGAACCAGGCCTCTCAGTG 60.0 0.23
mm4_intergenic_Gm25778|Ppapdc1a_chr7_129129292_R GTCTCGTGGGCTCGGCTGGGAGTGGAGACCAAACA 59.2 0.23
mm4_intron_Arhgap42_chr9_9054647_F TCGTCGGCAGCGTCTGCTGGACTCCAAAGCTGAG 59.9 0.23
mm4_intron_Arhgap42_chr9_9054647_R GTCTCGTGGGCTCGGTCATAATCTGCTTCCATGTGGGA 59.5 0.23
mm4_intergenic_Cmklr1|1700069L16Rik_chr5_113684516_F TCGTCGGCAGCGTCGAGCGTGAGTGTCCTTCAGT 59.6 0.23
mm4_intergenic_Cmklr1|1700069L16Rik_chr5_113684516_R GTCTCGTGGGCTCGGTCCAGTGCCAGGAGAGCTAA 60.2 0.23
mm2_exon_Synpo_chr18_60601424_F Primer3: not found at this Tm N.d. 0.23
mm2_exon_Synpo_chr18_60601424_R Primer3: not found at this Tm N.d. 0.23
mm4_intron_Grin1_chr2_25308733_F TCGTCGGCAGCGTCAGCGTGCGTGTACTGGTAAT 59.7 0.23
mm4_intron_Grin1_chr2_25308733_R GTCTCGTGGGCTCGGTGGAATTAGCTGCCCCACTC 59.7 0.23
mm3_exon_Shbg_chr11_69615559_F TCGTCGGCAGCGTCTACCTGGTAAAGCCCCAAGG 59.0 0.22
mm3_exon_Shbg_chr11_69615559_R GTCTCGTGGGCTCGGGGTCTTGAGCCAAGGACCAA 59.8 0.22
mm4_intergenic_Olfr373|Olfr374_chr8_72103180_F Primer3: not found at this Tm N.d. 0.22
mm4_intergenic_Olfr373|Olfr374_chr8_72103180_R Primer3: not found at this Tm N.d. 0.22
mm4_intergenic_Cyp2b13|Cyp2b9_chr7_26139183_F Primer3: not found at this Tm N.d. 0.21
mm4_intergenic_Cyp2b13|Cyp2b9_chr7_26139183_R Primer3: not found at this Tm N.d. 0.21
mm4_intergenic_Cyp2b10|Cyp2b13_chr7_26019481_F Primer3: not found at this Tm N.d. 0.21
mm4_intergenic_Cyp2b10|Cyp2b13_chr7_26019481_R Primer3: not found at this Tm N.d. 0.21
mm4_intron_Galnt2_chr8_124287509_F Primer3: not found at this Tm N.d. 0.21
mm4_intron_Galnt2_chr8_124287509_R Primer3: not found at this Tm N.d. 0.21
mm2_intron_Slc41a2_chr10_83286591_F Primer3: not found at this Tm N.d. 0.20
mm2_intron_Slc41a2_chr10_83286591_R Primer3: not found at this Tm N.d. 0.20
mm3_intergenic_Gm13232|Gm13244_chr4_145745018_F Primer3: not found at this Tm N.d. 0.20
mm3_intergenic_Gm13232|Gm13244_chr4_145745018_R Primer3: not found at this Tm N.d. 0.20
mm4_intergenic_4933407E24Rik|3100002H09Rik_chr4_124594999_F Primer3: not found at this Tm N.d. 0.20
mm4_intergenic_4933407E24Rik|3100002H09Rik_chr4_124594999_R Primer3: not found at this Tm N.d. 0.20
mm4_intergenic_Olfr771|Gm25807_chr10_129170289_F Primer3: not found at this Tm N.d. 0.19
mm4_intergenic_Olfr771|Gm25807_chr10_129170289_R Primer3: not found at this Tm N.d. 0.19
mm3_intergenic_Atp2c1|Gm5621_chr9_105553784_F Primer3: not found at this Tm N.d. 0.18
mm3_intergenic_Atp2c1|Gm5621_chr9_105553784_R Primer3: not found at this Tm N.d. 0.18
mm4_intron_Ulk1_chr5_110796545_F TCGTCGGCAGCGTCGCTCAGGAGACTTGGGACAC 60.0 0.18
mm4_intron_Ulk1_chr5_110796545_R GTCTCGTGGGCTCGGCTGGAGGCCCTCTGTAGAGT 60.0 0.18
mm4_intergenic_Gm23980|Gm22810_chr15_24756776_F TCGTCGGCAGCGTCGCAAGGTAGCCATGAAGAACC 59.2 0.18
mm4_intergenic_Gm23980|Gm22810_chr15_24756776_R GTCTCGTGGGCTCGGCTGAATGCAGGAGCTGAGAGT 59.7 0.18
mm4_intron_Hoxb3_chr11_96326662_F TCGTCGGCAGCGTCTCTGGACAATCAAAGCTGACCA 59.8 0.18
mm4_intron_Hoxb3_chr11_96326662_R GTCTCGTGGGCTCGGCTCGGAGGTTTCGAGGCAG 60.1 0.18
mm4_intron_Bai2_chr4_130016227_F Primer3: not found at this Tm N.d. 0.17
mm4_intron_Bai2_chr4_130016227_R Primer3: not found at this Tm N.d. 0.17
mm4_intergenic_Gm22227|Zfp703_chr8_26373151_F Primer3: not found at this Tm N.d. 0.17
mm4_intergenic_Gm22227|Zfp703_chr8_26373151_R Primer3: not found at this Tm N.d. 0.17
mm4_intergenic_Gm22527|Rnf144a_chr12_26243032_F Primer3: not found at this Tm N.d. 0.16
mm4_intergenic_Gm22527|Rnf144a_chr12_26243032_R Primer3: not found at this Tm N.d. 0.16
mm4_intron_Tpd52_chr3_8993109_F TCGTCGGCAGCGTCTTGGAGTCTTCTAGCACCGC 59.7 0.16
mm4_intron_Tpd52_chr3_8993109_R GTCTCGTGGGCTCGGAGAACGGATGCCTCAGGTTG 60.0 0.16
mm4_intron_Rbms3_chr9_116926053_F Primer3: not found at this Tm N.d. 0.15
mm4_intron_Rbms3_chr9_116926053_R Primer3: not found at this Tm N.d. 0.15
mm4_intergenic_Nck2|1500015O10Rik_chr1_43703404_F Primer3: not found at this Tm N.d. 0.15
mm4_intergenic_Nck2|1500015O10Rik_chr1_43703404_R Primer3: not found at this Tm N.d. 0.15
mm4_intron_Susd4_chr1_182834707_F Primer3: not found at this Tm N.d. 0.14
mm4_intron_Susd4_chr1_182834707_R Primer3: not found at this Tm N.d. 0.14
mm4_exon_Mirg/Mir377_chr12_109740401_F TCGTCGGCAGCGTCGGGGCTCATCGGAGGTAATG 59.9 0.13
mm4_exon_Mirg/Mir377_chr12_109740401_R GTCTCGTGGGCTCGGAATCAAACGTCAGCACAGGC 59.4 0.13
mm4_intergenic_Gm7180|Pde8a_chr7_81237129_F Primer3: not found at this Tm N.d. 0.13
mm4_intergenic_Gm7180|Pde8a_chr7_81237129_R Primer3: not found at this Tm N.d. 0.13
mm4_intron_Tmprss3_chr17_31183394_F TCGTCGGCAGCGTCTGTTTGCCTAGCACCTGATGT 59.9 0.13
mm4_intron_Tmprss3_chr17_31183394_R GTCTCGTGGGCTCGGAGAGGCCTTGCTGCAATGAA 60.2 0.13
mm4_intron_Thsd7a_chr6_12383784_F Primer3: not found at this Tm N.d. 0.13
mm4_intron_Thsd7a_chr6_12383784_R Primer3: not found at this Tm N.d. 0.13
mm4_intron_Dlgap3_chr4_127172323_F TCGTCGGCAGCGTCTTCAGTCTGAAGGCTGCTGG 59.9 0.12
mm4_intron_Dlgap3_chr4_127172323_R GTCTCGTGGGCTCGGTTAGCAACGATGAGCTGGCA 60.0 0.12
mm4_intergenic_Gm10040|Gm17641/1110032F04Rik_chr3_68818434_F TCGTCGGCAGCGTCGGTTCAACCCAGGGCTGTAG 60.3 0.12
mm4_intergenic_Gm10040|Gm17641/1110032F04Rik_chr3_68818434_R GTCTCGTGGGCTCGGTGGCACCTGTGACCTTCAAA 59.7 0.12
mm4_intergenic_Gm26651|Diras2_chr13_52069973_F Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm26651|Diras2_chr13_52069973_R Primer3: not found at this Tm N.d. 0.12
mm3_intron_Sdk2_chr11_113805851_F Primer3: not found at this Tm N.d. 0.12
mm3_intron_Sdk2_chr11_113805851_R Primer3: not found at this Tm N.d. 0.12
mm4_intron_Cntn6_chr6_104501777_F Primer3: not found at this Tm N.d. 0.12
mm4_intron_Cntn6_chr6_104501777_R Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_4933405L10Rik|Gfod2_chr8_105710958_F TCGTCGGCAGCGTCCTGTAGCAGGTGAGCCAGAC 60.1 0.12
mm4_intergenic_4933405L10Rik|Gfod2_chr8_105710958_R GTCTCGTGGGCTCGGATCCCTAACCAAAGCCGTCT 58.7 0.12
mm4_intergenic_Gm22521|Gm25702_chr15_62829785_F Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm22521|Gm25702_chr15_62829785_R Primer3: not found at this Tm N.d. 0.12
mm4_intron_Ifngr2_chr16_91560332_F Primer3: not found at this Tm N.d. 0.12
mm4_intron_Ifngr2_chr16_91560332_R Primer3: not found at this Tm N.d. 0.12
mm4_intron_Papss1_chr3_131614055_F TCGTCGGCAGCGTCTACACTGTGATTGCCCCGTG 60.3 0.11
mm4_intron_Papss1_chr3_131614055_R GTCTCGTGGGCTCGGGCCAAGAGGAGTGTAGTGGC 60.3 0.11
mm4_intron_Nxnl2_chr13_51172982_F TCGTCGGCAGCGTCAGTTGAGATGGTGCTGGTGG 59.9 0.11
mm4_intron_Nxnl2_chr13_51172982_R GTCTCGTGGGCTCGGCTACCATCCTCGCCAGCAAC 60.8 0.11
mm4_intergenic_Ctnna3|Gm22829_chr10_65033235_F Primer3: not found at this Tm N.d. 0.11
mm4_intergenic_Ctnna3|Gm22829_chr10_65033235_R Primer3: not found at this Tm N.d. 0.11
mm4_intergenic_Vmn1r-ps149|Gm26384_chr17_21272596_F Primer3: not found at this Tm N.d. 0.11
mm4_intergenic_Vmn1r-ps149|Gm26384_chr17_21272596_R Primer3: not found at this Tm N.d. 0.11
mm3_intron_Fbxo21_chr5_118003644_F TCGTCGGCAGCGTCTTCCTCATTGCCCAGTGGTC 59.9 0.11
mm3_intron_Fbxo21_chr5_118003644_R GTCTCGTGGGCTCGGGTCCTGGTGCAGCAGAGATT 60.0 0.11
mm4_intergenic_Ucn3|Gm22460_chr13_3947836_F TCGTCGGCAGCGTCCTCTCTTGAAGCCAGACCCC 59.7 0.10
mm4_intergenic_Ucn3|Gm22460_chr13_3947836_R GTCTCGTGGGCTCGGAGTCCTGTCAGCCAATGGAA 58.9 0.10
mm4_intergenic_Gm22000|Myl4/Gm22000_chr11_104559907_F Primer3: not found at this Tm N.d. 0.10
mm4_intergenic_Gm22000|Myl4/Gm22000_chr11_104559907_R Primer3: not found at this Tm N.d. 0.10
mm4_intron_Myh3_chr11_67095861_F TCGTCGGCAGCGTCGGCTGCGTAGGGTTTACAGT 60.0 0.10
mm4_intron_Myh3_chr11_67095861_R GTCTCGTGGGCTCGGCATTGGGCCAAATGGGGTTG 60.0 0.10
mm4_exon_Tead4_chr6_128271025_F Primer3: not found at this Tm N.d. 0.10
mm4_exon_Tead4_chr6_128271025_R Primer3: not found at this Tm N.d. 0.10
mm4_intergenic_Slc25a20|Prkar2a_chr9_108688561_F Primer3: not found at this Tm N.d. 0.10
mm4_intergenic_Slc25a20|Prkar2a_chr9_108688561_R Primer3: not found at this Tm N.d. 0.10
mm4_intergenic_mmu-mir-6393|Zdhhc25_chr15_87884816_F TCGTCGGCAGCGTCCAGGAGAGTCAGAGGAGCCT 60.0 0.10
mm4_intergenic_mmu-mir-6393|Zdhhc25_chr15_87884816_R GTCTCGTGGGCTCGGTGGCATAATGGCTTCTCAGCT 59.7 0.10
mm4_intergenic_Gm13237|Gm13233_chr4_146579900_F Primer3: not found at this Tm N.d. 0.10
mm4_intergenic_Gm13237|Gm13233_chr4_146579900_R Primer3: not found at this Tm N.d. 0.10
mm4_intergenic_Gm13167|Gm13169_chr4_146239255_F Primer3: not found at this Tm N.d. 0.10
mm4_intergenic_Gm13167|Gm13169_chr4_146239255_R Primer3: not found at this Tm N.d. 0.10
mm4_intergenic_Lep|Gm25589_chr6_29104937_F TCGTCGGCAGCGTCGATTTTCAGCAAGCCCAAAGC 58.9 0.10
mm4_intergenic_Lep|Gm25589_chr6_29104937_R GTCTCGTGGGCTCGGTCCTCCTCCCCACTCATCTG 60.0 0.10
mm4_exon_Olr1_chr6_129485759_F Primer3: not found at this Tm N.d. 0.10
mm4_exon_Olr1_chr6_129485759_R Primer3: not found at this Tm N.d. 0.10
mm4_intergenic_n-R5s220|Cacna1e_chr1_154382958_F TCGTCGGCAGCGTCTCCCTTGCTTCCCAAATGTT 57.6 0.10
mm4_intergenic_n-R5s220|Cacna1e_chr1_154382958_R GTCTCGTGGGCTCGGAGTGTAAGGTGCAAGGGTCA 58.8 0.10
mm4_intergenic_Gm16065|Tbx5_chr5_119795634_F Primer3: not found at this Tm N.d. 0.09
mm4_intergenic_Gm16065|Tbx5_chr5_119795634_R Primer3: not found at this Tm N.d. 0.09
mm4_intergenic_Gstt1|Gstt4_chr10_75811428_F Primer3: not found at this Tm N.d. 0.09
mm4_intergenic_Gstt1|Gstt4_chr10_75811428_R Primer3: not found at this Tm N.d. 0.09
mm4_intergenic_Gm7275|Gm7204_chr16_48160036_F Primer3: not found at this Tm N.d. 0.09
mm4_intergenic_Gm7275|Gm7204_chr16_48160036_R Primer3: not found at this Tm N.d. 0.09
mm4_intergenic_Fbxo7|Gm24430_chr10_86037368_F Primer3: not found at this Tm N.d. 0.08
mm4_intergenic_Fbxo7|Gm24430_chr10_86037368_R Primer3: not found at this Tm N.d. 0.08
mm4_intron_Parva_chr7_112557589_F TCGTCGGCAGCGTCCCTAGCTCTCACACCCTCCT 60.0 0.08
mm4_intron_Parva_chr7_112557589_R GTCTCGTGGGCTCGGCTAGACTGCTGGTGACCGAC 59.8 0.08
mm4_intron_Pik3c2g_chr6_139912555_F Primer3: not found at this Tm N.d. 0.08
mm4_intron_Pik3c2g_chr6_139912555_R Primer3: not found at this Tm N.d. 0.08
mm4_intron_Map3k4_chr17_12235355_F TCGTCGGCAGCGTCTGAGTGCTGAGGGAGTGGTA 59.8 0.08
mm4_intron_Map3k4_chr17_12235355_R GTCTCGTGGGCTCGGGCTGTACCATCCCAGACAGG 59.8 0.08
mm4_intron_Stmn2_chr3_8551222_F TCGTCGGCAGCGTCGTAGGGTCAGAACTGGGTGC 60.0 0.08
mm4_intron_Stmn2_chr3_8551222_R GTCTCGTGGGCTCGGGAAGTAGAGGCTCCTCCCCA 60.0 0.08
mm4_exon_Pklr/Hcn3_chr3_89146130_F Primer3: not found at this Tm N.d. 0.08
mm4_exon_Pklr/Hcn3_chr3_89146130_R Primer3: not found at this Tm N.d. 0.08
mm4_intron_Kcnh1_chr1_192445287_F TCGTCGGCAGCGTCTGGCTGTTCTAGGTGCACAG 59.9 0.08
mm4_intron_Kcnh1_chr1_192445287_R GTCTCGTGGGCTCGGAAATGAGGGGTAAGCACGCA 59.9 0.08
mm4_intron_Scai_chr2_39077493_F Primer3: not found at this Tm N.d. 0.08
mm4_intron_Scai_chr2_39077493_R Primer3: not found at this Tm N.d. 0.08
mm4_intergenic_Gm16155|Tmcc3_chr10_94426566_F TCGTCGGCAGCGTCGGTGAGAGATGCAGGCCAAT 60.1 0.07
mm4_intergenic_Gm16155|Tmcc3_chr10_94426566_R GTCTCGTGGGCTCGGTCTGTCCCTATTCAGCGGGT 60.3 0.07
mm4_intron_Snx9_chr17_5925817_F Primer3: not found at this Tm N.d. 0.07
mm4_intron_Snx9_chr17_5925817_R Primer3: not found at this Tm N.d. 0.07
mm4_intergenic_Gm5218|Gm23880_chr15_81523282_F TCGTCGGCAGCGTCCTCCGCTGCAAGCCATTCT 60.7 0.07
mm4_intergenic_Gm5218|Gm23880_chr15_81523282_R GTCTCGTGGGCTCGGGGAGCTCCGCCCTCATCTAG 61.5 0.07
mm4_intergenic_Erv3|Lamr1-ps1_chr2_131892620_F TCGTCGGCAGCGTCTGGTGTGCAAAACCCAGACT 60.0 0.07
mm4_intergenic_Erv3|Lamr1-ps1_chr2_131892620_R GTCTCGTGGGCTCGGTGGCTCCTTTCTCCGGTTTC 59.9 0.07
mm4_intron_Ccdc65_chr15_98712635_F Primer3: not found at this Tm N.d. 0.07
mm4_intron_Ccdc65_chr15_98712635_R Primer3: not found at this Tm N.d. 0.07
mm4_intergenic_Gm12462|Pax5_chr4_44677301_F TCGTCGGCAGCGTCTGCCTGCCACTCCTTTTCAT 59.8 0.06
mm4_intergenic_Gm12462|Pax5_chr4_44677301_R GTCTCGTGGGCTCGGCCTCATCCCCTGGCATCATC 59.9 0.06
mm4_intergenic_Zfp787|Zfp444_chr7_6169453_F Primer3: not found at this Tm N.d. 0.06
mm4_intergenic_Zfp787|Zfp444_chr7_6169453_R Primer3: not found at this Tm N.d. 0.06
mm4_intergenic_BC049730|Gm4763_chr7_24717998_F Primer3: not found at this Tm N.d. 0.06
mm4_intergenic_BC049730|Gm4763_chr7_24717998_R Primer3: not found at this Tm N.d. 0.06
mm4_intron_Paqr5_chr9_61986807_F Primer3: not found at this Tm N.d. 0.06
mm4_intron_Paqr5_chr9_61986807_R Primer3: not found at this Tm N.d. 0.06
mm4_intron_Myh9_chr15_77804081_F Primer3: not found at this Tm N.d. 0.06
mm4_intron_Myh9_chr15_77804081_R Primer3: not found at this Tm N.d. 0.06
mm4_exon_Dnah2_chr11_69496625_F TCGTCGGCAGCGTCAGATGGTGGCAAAGAGGTGG 59.9 0.06
mm4_exon_Dnah2_chr11_69496625_R GTCTCGTGGGCTCGGCTCGAGGCTTGTGTCTCCAG 60.1 0.06
mm4_intergenic_Gm20628/Hist2h3b/Hist2h2bb|Hist2h2bb_chr3_96276238_F TCGTCGGCAGCGTCCCTGCTCATTCGTCGGGAAT 60.1 0.06
mm4_intergenic_Gm20628/Hist2h3b/Hist2h2bb|Hist2h2bb_chr3_96276238_R GTCTCGTGGGCTCGGGGTATTCACCACACTGGCCT 59.6 0.06
mm4_intergenic_Gm8994|Gm15476_chr6_136359090_F Primer3: not found at this Tm N.d. 0.06
mm4_intergenic_Gm8994|Gm15476_chr6_136359090_R Primer3: not found at this Tm N.d. 0.06
mm4_intergenic_Zcchc6|Gas1_chr13_60014358_F TCGTCGGCAGCGTCGTGGGCTTTGCGAAAACTCC 60.3 0.05
mm4_intergenic_Zcchc6|Gas1_chr13_60014358_R GTCTCGTGGGCTCGGGGGTTGGAGCCATCAGTCTG 60.3 0.05
mm4_intergenic_1110059M19Rik|Gm14606_chrX_45153346_F Primer3: not found at this Tm N.d. 0.05
mm4_intergenic_1110059M19Rik|Gm14606_chrX_45153346_R Primer3: not found at this Tm N.d. 0.05
mm4_intergenic_Gm10110|Gm25495_chr14_89926474_F Primer3: not found at this Tm N.d. 0.05
mm4_intergenic_Gm10110|Gm25495_chr14_89926474_R Primer3: not found at this Tm N.d. 0.05
mm4_intron_Gm16168_chr17_10564418_F TCGTCGGCAGCGTCAGCAAACCTAGTGACCAGCA 59.2 0.05
mm4_intron_Gm16168_chr17_10564418_R GTCTCGTGGGCTCGGCAGATGTCAGAACAGGCCGA 59.7 0.05
mm4_intergenic_Runx1|AC124326.1_chr16_92980229_F Primer3: not found at this Tm N.d. 0.05
mm4_intergenic_Runx1|AC124326.1_chr16_92980229_R Primer3: not found at this Tm N.d. 0.05
mm4_intron_Edil3_chr13_89115985_F Primer3: not found at this Tm N.d. 0.05
mm4_intron_Edil3_chr13_89115985_R Primer3: not found at this Tm N.d. 0.05
mm3_intergenic_CT030233.1|Gm20675_chr14_67189164_F Primer3: not found at this Tm N.d. 0.05
mm3_intergenic_CT030233.1|Gm20675_chr14_67189164_R Primer3: not found at this Tm N.d. 0.05
mm4_intergenic_Gm12439|Gm12438_chr4_48720103_F Primer3: not found at this Tm N.d. 0.05
mm4_intergenic_Gm12439|Gm12438_chr4_48720103_R Primer3: not found at this Tm N.d. 0.05
mm4_intron_Mccc2_chr13_99951085_F Primer3: not found at this Tm N.d. 0.05
mm4_intron_Mccc2_chr13_99951085_R Primer3: not found at this Tm N.d. 0.05
mm3_intron_Tia1_chr6_86408623_F Primer3: not found at this Tm N.d. 0.04
mm3_intron_Tia1_chr6_86408623_R Primer3: not found at this Tm N.d. 0.04
mm3_intergenic_Gm26563|Gm26563/Suz12_chr11_80021819_F Primer3: not found at this Tm N.d. 0.04
mm3_intergenic_Gm26563|Gm26563/Suz12_chr11_80021819_R Primer3: not found at this Tm N.d. 0.04
mm4_intergenic_Adamtsl5|Plk5_chr10_80350887_F Primer3: not found at this Tm N.d. 0.04
mm4_intergenic_Adamtsl5|Plk5_chr10_80350887_R Primer3: not found at this Tm N.d. 0.04
mm4_intergenic_Gm22277|Lrrc30_chr17_67629387_F Primer3: not found at this Tm N.d. 0.04
mm4_intergenic_Gm22277|Lrrc30_chr17_67629387_R Primer3: not found at this Tm N.d. 0.04
mm4_intron_Lep_chr6_29070426_F TCGTCGGCAGCGTCAAGAGTCATCAGGGCTGCAC 60.0 0.04
mm4_intron_Lep_chr6_29070426_R GTCTCGTGGGCTCGGCATTAGGGTCAAAGCCGTGC 59.5 0.04
mm2_intergenic_E030044B06Rik|mmu-mir-8092/Zfp518a_chr19_40874233_F Primer3: not found at this Tm N.d. 0.04
mm2_intergenic_E030044B06Rik|mmu-mir-8092/Zfp518a_chr19_40874233_R Primer3: not found at this Tm N.d. 0.04
mm4_intergenic_5S_rRNA|Rasa2_chr9_96526758_F TCGTCGGCAGCGTCCTCCCTGGGTTCCTTAGGGA 59.9 0.04
mm4_intergenic_5S_rRNA|Rasa2_chr9_96526758_R GTCTCGTGGGCTCGGACCTCCCAAGATCTGCTCCT 59.9 0.04
mm3_intergenic_Naif1|Gm13412_chr2_32517275_F Primer3: not found at this Tm N.d. 0.04
mm3_intergenic_Naif1|Gm13412_chr2_32517275_R Primer3: not found at this Tm N.d. 0.04
mm3_intergenic_Gm4487|Gm24073_chr14_113620142_F Primer3: not found at this Tm N.d. 0.04
mm3_intergenic_Gm4487|Gm24073_chr14_113620142_R Primer3: not found at this Tm N.d. 0.04
mm4_intron_Ak9_chr10_41380811_F Primer3: not found at this Tm N.d. 0.04
mm4_intron_Ak9_chr10_41380811_R Primer3: not found at this Tm N.d. 0.04
mm4_intron_Ext2_chr2_93797405_F TCGTCGGCAGCGTCGGATCGCTCTTCCTGTCCAG 59.8 0.04
mm4_intron_Ext2_chr2_93797405_R GTCTCGTGGGCTCGGGGCCCACCCTGAAGTTGTTA 59.8 0.04
mm4_intron_Magi1_chr6_93929116_F TCGTCGGCAGCGTCACGCTAGCTGACCCAGTTTC 60.0 0.04
mm4_intron_Magi1_chr6_93929116_R GTCTCGTGGGCTCGGTGGGATACTTCCAAGTCTGTGG 59.4 0.04
mm3_intergenic_Tm9sf4|Tspyl3_chr2_153220020_F Primer3: not found at this Tm N.d. 0.03
mm3_intergenic_Tm9sf4|Tspyl3_chr2_153220020_R Primer3: not found at this Tm N.d. 0.03
mm4_intergenic_Fars2|Gm26395_chr13_36555097_F TCGTCGGCAGCGTCTGTGGCTTCTGTCCAGTGTC 59.8 0.03
mm4_intergenic_Fars2|Gm26395_chr13_36555097_R GTCTCGTGGGCTCGGTGTAGGGACCAATCTGCAGC 59.7 0.03
mm3_intergenic_Kctd3|Kcnk2_chr1_189088959_F Primer3: not found at this Tm N.d. 0.03
mm3_intergenic_Kctd3|Kcnk2_chr1_189088959_R Primer3: not found at this Tm N.d. 0.03
mm4_intergenic_Bmper|Npsr1_chr9_23697125_F TCGTCGGCAGCGTCTACAAACTCCCCTGCACCAC 59.8 0.03
mm4_intergenic_Bmper|Npsr1_chr9_23697125_R GTCTCGTGGGCTCGGGGCACCTCCAGCATCAGATT 60.1 0.03
mm4_intergenic_Gm26104|Cep170_chr1_176715452_F Primer3: not found at this Tm N.d. 0.03
mm4_intergenic_Gm26104|Cep170_chr1_176715452_R Primer3: not found at this Tm N.d. 0.03
mm4_exon_Amz1_chr5_140752245_F TCGTCGGCAGCGTCCGCCATGGAGGAGTATGGAC 59.9 0.03
mm4_exon_Amz1_chr5_140752245_R GTCTCGTGGGCTCGGCCCTGTGAACATCTCCCACC 60.0 0.03
mm4_intergenic_Tmem131|Rpl12-ps1_chr1_36943091_F Primer3: not found at this Tm N.d. 0.03
mm4_intergenic_Tmem131|Rpl12-ps1_chr1_36943091_R Primer3: not found at this Tm N.d. 0.03
mm4_intergenic_Gm5759|Gm8880_chrX_88909406_F Primer3: not found at this Tm N.d. 0.03
mm4_intergenic_Gm5759|Gm8880_chrX_88909406_R Primer3: not found at this Tm N.d. 0.03
mm4_intron_Atxn1_chr13_45818687_F Primer3: not found at this Tm N.d. 0.02
mm4_intron_Atxn1_chr13_45818687_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Rbm33|Shh/9530036O11Rik_chr5_28456604_F TCGTCGGCAGCGTCAGAGTTGGAAATGGGCAGGG 59.9 0.02
mm4_intergenic_Rbm33|Shh/9530036O11Rik_chr5_28456604_R GTCTCGTGGGCTCGGGCCCTCCTCATTGGAAGACC 60.1 0.02
mm3_intergenic_5430435G22Rik|Slc26a9_chr1_131742805_F TCGTCGGCAGCGTCGGGCTCCAAGACAGTGTTCA 59.8 0.02
mm3_intergenic_5430435G22Rik|Slc26a9_chr1_131742805_R GTCTCGTGGGCTCGGGTCGAGGTGTAGCCAGGATG 59.8 0.02
mm4_intergenic_Acaa2|Gm24559_chr18_74901972_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Acaa2|Gm24559_chr18_74901972_R Primer3: not found at this Tm N.d. 0.02
mm4_intron_Zfyve27_chr19_42174795_F TCGTCGGCAGCGTCGTCTGAGGCTATGCGGAGAG 59.6 0.02
mm4_intron_Zfyve27_chr19_42174795_R GTCTCGTGGGCTCGGCAGACCAGTGATCCCTCTGC 59.8 0.02
mm4_intron_E230008N13Rik_chr4_45919193_F Primer3: not found at this Tm N.d. 0.02
mm4_intron_E230008N13Rik_chr4_45919193_R Primer3: not found at this Tm N.d. 0.02
mm4_intron_Plxnc1_chr10_94802828_F Primer3: not found at this Tm N.d. 0.02
mm4_intron_Plxnc1_chr10_94802828_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Gfra2|Gm9192_chr14_71740223_F TCGTCGGCAGCGTCGGTTCGCACAAGACATGTCAC 60.0 0.02
mm4_intergenic_Gfra2|Gm9192_chr14_71740223_R GTCTCGTGGGCTCGGAAAAGCATCTGGAGTGGGCA 59.8 0.02
mm4_exon_Rtn2_chr7_19287094_F TCGTCGGCAGCGTCCTGGAGGAACTGAGGCTGC 60.0 0.02
mm4_exon_Rtn2_chr7_19287094_R GTCTCGTGGGCTCGGCTGGCCTCCAATCCATCAGG 60.1 0.02
mm4_intergenic_Ppargc1b|Gm25301_chr18_61397595_F TCGTCGGCAGCGTCCTAAGCCTGGGTTAGCCACC 60.1 0.02
mm4_intergenic_Ppargc1b|Gm25301_chr18_61397595_R GTCTCGTGGGCTCGGGTCCAGCTTCTTCCCCACAG 60.3 0.02
mm4_intergenic_Prdx6|Tnfsf4_chr1_161288470_F TCGTCGGCAGCGTCTCTCTGGCTGTGCTCTCTCT 59.9 0.02
mm4_intergenic_Prdx6|Tnfsf4_chr1_161288470_R GTCTCGTGGGCTCGGATTGGGAGTGAGCCTGTGAC 59.6 0.02
mm4_intron_Celsr1_chr15_85942654_F TCGTCGGCAGCGTCAAACGCAGGGTAGTGTCACT 59.2 0.02
mm4_intron_Celsr1_chr15_85942654_R GTCTCGTGGGCTCGGGGGGTGTTGAGGAGAACTGT 59.2 0.02
mm3_intron_Ncor2_chr5_125044176_F Primer3: not found at this Tm N.d. 0.02
mm3_intron_Ncor2_chr5_125044176_R Primer3: not found at this Tm N.d. 0.02
mm4_exon_Gm10612/Cbfa2t3_chr8_122698856_F Primer3: not found at this Tm N.d. 0.01
mm4_exon_Gm10612/Cbfa2t3_chr8_122698856_R Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Gm17118|Cacna2d2_chr9_107460850_F TCGTCGGCAGCGTCCCAGCTAAGTCTGCCAGTCC 60.1 0.01
mm4_intergenic_Gm17118|Cacna2d2_chr9_107460850_R GTCTCGTGGGCTCGGGAGCTTGGGGCAGACAAGAT 60.0 0.01
mm4_intergenic_Nbea|Gm25727_chr3_56311846_F Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Nbea|Gm25727_chr3_56311846_R Primer3: not found at this Tm N.d. 0.01
mm4_intron_Slc4a5_chr6_83272634_F Primer3: not found at this Tm N.d. 0.01
mm4_intron_Slc4a5_chr6_83272634_R Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Reep5|Pkd2l2_chr18_34405504_F Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Reep5|Pkd2l2_chr18_34405504_R Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Slc1a6|Olfr1356_chr10_78837698_F Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Slc1a6|Olfr1356_chr10_78837698_R Primer3: not found at this Tm N.d. 0.01
mm4_intron_Tnfaip1_chr11_78526135_F Primer3: not found at this Tm N.d. 0.01
mm4_intron_Tnfaip1_chr11_78526135_R Primer3: not found at this Tm N.d. 0.01
mm4_intron_Sfxn5_chr6_85220072_F TCGTCGGCAGCGTCGAGGGAGCTGGATGTGTACG 59.8 0.01
mm4_intron_Sfxn5_chr6_85220072_R GTCTCGTGGGCTCGGCTTCGTGGGCTCATTCCCTT 60.0 0.01
mm4_intron_Lasp1_chr11_97808236_F Primer3: not found at this Tm N.d. 0.01
mm4_intron_Lasp1_chr11_97808236_R Primer3: not found at this Tm N.d. 0.01
mm3_intron_Ahdc1_chr4_133031804_F Primer3: not found at this Tm N.d. 0.01
mm3_intron_Ahdc1_chr4_133031804_R Primer3: not found at this Tm N.d. 0.01
mm4_intron_Smyd3_chr1_179290133_F Primer3: not found at this Tm N.d. 0.01
mm4_intron_Smyd3_chr1_179290133_R Primer3: not found at this Tm N.d. 0.01
mm3_intron_Ano10_chr9_122265208_F TCGTCGGCAGCGTCTGGCTACCACTTTCACACCC 59.8 0.01
mm3_intron_Ano10_chr9_122265208_R GTCTCGTGGGCTCGGCCTTCACAGTAGCTTGCCCT 59.6 0.01
mm4_intron_Atp8b5_chr4_43323622_F Primer3: not found at this Tm N.d. 0.00
mm4_intron_Atp8b5_chr4_43323622_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Irs4|Irs4/Gm15295_chrX_141720753_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Irs4|Irs4/Gm15295_chrX_141720753_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Ptgfrn|Gm12486_chr3_101131475_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Ptgfrn|Gm12486_chr3_101131475_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Mbtps2|Mbtps2/Yy2_chrX_157587082_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Mbtps2|Mbtps2/Yy2_chrX_157587082_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Nhp2l1|4930407I10Rik_chr15_82058114_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Nhp2l1|4930407I10Rik_chr15_82058114_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Nlgn2|Tmem256_chr11_69837853_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Nlgn2|Tmem256_chr11_69837853_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Rassf5|Ikbke_chr1_131251285_F TCGTCGGCAGCGTCCTCTCTGGCCACTCTCGTTC 59.8 0.00
mm4_intergenic_Rassf5|Ikbke_chr1_131251285_R GTCTCGTGGGCTCGGACTGCCTTTTAGAGCCTTGGT 59.5 0.00
mm4_exon_Nhlrc4/Pigq_chr17_25944526_F TCGTCGGCAGCGTCCACAGGTGTCTGGGTGTCTG 60.2 0.00
mm4_exon_Nhlrc4/Pigq_chr17_25944526_R GTCTCGTGGGCTCGGCTCTGCATGCGATTTGAGCC 59.9 0.00
mm4_intron_Ssbp4_chr8_70604885_F TCGTCGGCAGCGTCGCATCAGCATGTGCCATCAG 59.9 0.00
mm4_intron_Ssbp4_chr8_70604885_R GTCTCGTGGGCTCGGTGAAAATGCCAGGAGGACCC 59.9 0.00
mm4_intron_Mrc2_chr11_105331462_F TCGTCGGCAGCGTCTATGGGCCCTGCAATGTTCC 60.3 0.00
mm4_intron_Mrc2_chr11_105331462_R GTCTCGTGGGCTCGGCCCTCTGTGTTGACCTCTGC 60.3 0.00
mm4_intron_Mgll_chr6_88769972_F TCGTCGGCAGCGTCGCAGCTGCTCCTATGACTCA 59.5 0.00
mm4_intron_Mgll_chr6_88769972_R GTCTCGTGGGCTCGGGGGCAGTAGATTCCTGTCCG 59.8 0.00
mm4_intron_L3mbtl3_chr10_26305159_F TCGTCGGCAGCGTCAGCAAGATGAAAGACACCCA 57.0 0.00
mm4_intron_L3mbtl3_chr10_26305159_R GTCTCGTGGGCTCGGTCGATGACCAGCCATTGCAT 60.1 0.00
mm4_intron_Hsd3b1_chr3_98859409_F Primer3: not found at this Tm N.d. 0.00
mm4_intron_Hsd3b1_chr3_98859409_R Primer3: not found at this Tm N.d. 0.00
mm4_intron_Gpr142_chr11_114802823_F Primer3: not found at this Tm N.d. 0.00
mm4_intron_Gpr142_chr11_114802823_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Tha1|Gm11725_chr11_117879894_F TCGTCGGCAGCGTCGCTGGATGAGTCTTGCGTCA 60.3 0.00
mm4_intergenic_Tha1|Gm11725_chr11_117879894_R GTCTCGTGGGCTCGGCCAGACACAAAGCAAGGTGC 59.9 0.00
mm4_intergenic_Oaf|D630033O11Rik_chr9_43255585_F TCGTCGGCAGCGTCTAGGAGGGGAGCAGAAGACC 60.0 0.00
mm4_intergenic_Oaf|D630033O11Rik_chr9_43255585_R GTCTCGTGGGCTCGGTTCACAGAAGCTCCTGCAGG 59.9 0.00
mm4_intergenic_Nkd2|Trip13_chr13_73876110_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Nkd2|Trip13_chr13_73876110_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Kif2b|Gm11498_chr11_91632238_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Kif2b|Gm11498_chr11_91632238_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm23649|Lclat1_chr17_73005997_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm23649|Lclat1_chr17_73005997_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Atp4b|Grk1_chr8_13403280_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Atp4b|Grk1_chr8_13403280_R Primer3: not found at this Tm N.d. 0.00
mm3_intron_Csmd2_chr4_128299766_F TCGTCGGCAGCGTCAGTGCAATTTCTACACCAATTGT 57.2 0.00
mm3_intron_Csmd2_chr4_128299766_R GTCTCGTGGGCTCGGAATAGTAACAGTGAGGGCCAC 57.3 0.00

Off-target amplicon sequences with primers

These only list off-targets that have primers in the table above. Primers underlined, off-targets in bold.

mm4_intron_Dgki_chr6_36850349 GGCTCTATCAGGCGTAGAACTGTTAACCATCTCAAGGTGAGATCCCACCATCTGGGGAGTCCGGCAGGTAGGCAGTTATT
GGGGCAGC
mm4_intergenic_Gm24696|Myc_chr15_61984263 TTCTAGGGTCTCTGCAGGCTCCCCAGATCTGGGTTGGCAATTCACTCCTCCCCCTTTCTGGGAAGTCCGGGTTTTCCCCA
ACCCCCCAATTCATGGCATATTCTCGCGTCTAGCGCCTTGATTTTCCCCACCCCA
mm4_intergenic_Gm26497|Piezo1_chr8_122548801 ATGGGACTAGGGCATCACCTTGCCTGACTTTCCAGATTAGGGGAGTGGCAAAGAGGCTAAGCACCCCA
mm4_intron_Ass1_chr2_31509703 GCGTGTTCATGCATGTGTGTGCACAGCCGTCCCCCTCCCCCGACTCCCCAGTGGAAGGGAGGAAGGGCTATTTGGGGCCC
TGGGCTCCACCGTAGTTGGTTTAATTACAGGTTTTCAACTGCAGGCC
mm4_exon_Nynrin/Gm26751_chr14_55871428 TGCAACTGGATCTTCAGCGTTCTCTGGGAGCTCCTGCCCCTCTGGAGAGTCCGGGGCTTCCTGTCATCTGACGGGGCTTC
ACTACCTCATC
mm4_intergenic_Cldn4|Cldn3/Wbscr25_chr5_134967530 AAACCGATTCCACCCTGACCTGGCCGCTCTCACCTCGCACCTTCCCAATGTACCCCCCCACGGGAGAGTCAGGTCACACC
CAGAACTATGGACTTTCTGGGTGAGAATACAACACCCTGTCACGCAAGAACAAGGC
mm4_intergenic_Gyg|Cpa3_chr3_20191592 TCCAATTCTATTCCCTTCTCAGCATCTATTAACTAGCTATAGATCCCCAGAATCTCCAGAAAGGGGAAGCGCTTCCNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNGATGTTCTGTGTTGATCTGCAGCCCAGGAATC
mm3_intron_Serpina1f_chr12_103691088 CTTCAGGCAGACTCAGGGTGTTCACATGCAATCATGTTCCTGCAGTGTTTGGCCACCCCTCTTACTCAGCCAGACATTCC
CCCAAGACCTGACTCTCGAGAGAGTGGGAGTCAGAGCAGCCGAGTACAACTGCTCAC
mm3_intergenic_Gm24291|Mon1b_chr8_113551665 GCGTTGTTCTGAACCGGAAGAAGTGAAGACAGCTTCCTCCTACCCCCATCTCAGAGCAGAGCAACTTCCTCTTGACTTTC
CCTCCCCTCCCCTGGAGAATCAGGGAGTAGCAGGTAACACTCCAAGGGAGGAAGGAGAGG
mm4_intron_Arhgef6_chrX_57249372 TAGGAAGAGGGGAGCATTCCCATTTTGTCAAAGCATCAAAATAAAGTGGTAAAGGGGGGGTATTTCTCCCTCCCCATGAA
GAGTCTGG
AGAGACAAGGAACAGATTCCTACCAACTAGAGCCAGAAAAGCAATCAGCCC
mm4_intergenic_Gm25778|Ppapdc1a_chr7_129129292 CAGAACCAGGCCTCTCAGTGATCATGAGTTTGACACTCTGGTCTCCTAAATCTGAGCGGGTGATACTCCCGACTCTCCAG
AGGGTCTCAG
AACTAGAGCCTGAAATGGCAAAGGAATCCATGTTTGGTCTCCACTCCCAG
mm4_intron_Arhgap42_chr9_9054647 TGCTGGACTCCAAAGCTGAGAACCTGCAACCCAGGAGAGGACCCACATTCTCGCAGGCTCTGCTCCTCCCCACTGCAAAG
TCTGG
TGGGATCCCACATGGAAGCAGATTATGA
mm4_intergenic_Cmklr1|1700069L16Rik_chr5_113684516 GAGCGTGAGTGTCCTTCAGTAGTCAATGACTCCAACGTCTGAGAAGCCTGCCCTGACTCTCCCGAGGGGAGTTGTGGGAG
TGCCTCCATCATTCTTGTTTAGCTCTCCTGGCACTGGA
mm4_intron_Grin1_chr2_25308733 AGCGTGCGTGTACTGGTAATGGGACAATTGGCACCTGCTTCCTGACTCTCCTGGGGGGAGGTGATGAAGTTGAGGATATT
ATTCAACACCACATCTGACCACTATCATCCTCAGAGTGGGGCAGCTAATTCCA
mm3_exon_Shbg_chr11_69615559 TACCTGGTAAAGCCCCAAGGGAGAGATGGCCCTGAGGGTGGGACCAGAGTCTCCAGAGGGAGGCAGGTCTCAAAAGAGAC
ATAGAAAGGACTTCCATCTTTGGTCCTTGGCTCAAGACC
mm4_intron_Ulk1_chr5_110796545 GCTCAGGAGACTTGGGACACCATGGTCTCTCTCTCTCTCTGGAGAGACAGGAGTGTACATGACTGTGTGGAAAGACAAGT
GCAGGGACTAAGCACCAGCACCACTCTACAGAGGGCCTCCAG
mm4_intergenic_Gm23980|Gm22810_chr15_24756776 GCAAGGTAGCCATGAAGAACCAACTCCCCCCCCCCCCCCCGAGAGTCTGGATGTTGCACAGTCACACTTACTCTCAGCTC
CTGCATTCAG
mm4_intron_Hoxb3_chr11_96326662 TCTGGACAATCAAAGCTGACCACCCCCCCCCCCTACAGAGTCTGGGCCCCGCCGGCGCCCCCTCCCCGTGGGGCAGGGGC
AGGCTTCCAGCACTTTCCGCAGGGGTCCTGCTGCCTCGAAACCTCCGAG
mm4_intron_Tpd52_chr3_8993109 TTGGAGTCTTCTAGCACCGCCCCTCCCCCACTGGAGAGCCAGGGCTCTGGGAGACCCATCCTGAGGCATCGGTCCTCCAG
CCCAGCTGAGAGCCGGGGCTCTGGGAGACCCAACCTGAGGCATCCGTTCT
mm4_exon_Mirg/Mir377_chr12_109740401 GGGGCTCATCGGAGGTAATGCCAGCCTCCCTAGAGGGTGGGAGTCAGGGAGCCAGAGCCGAGGGAGGCAGGGCTGCCTGG
GGAAGAGGCGCCCTGCTCAACTCTCGGCCTGTGCTGACGTTTGATT
mm4_intron_Tmprss3_chr17_31183394 TGTTTGCCTAGCACCTGATGTGTGTGTGGCCAGGCCTTGCTTCCCCACAGCCCTTCCCCTCTCAGGAGAGACGGGGGTTG
TAGACGTCTGACTTTCATTGCAGCAAGGCCTCT
mm4_intron_Dlgap3_chr4_127172323 TTCAGTCTGAAGGCTGCTGGCTCAGCGGGCCTCCTGCCGTCTGGGGAGTCTGGGTTTGAGCTGGGGTGAGGCGGTGGCAC
CATCGTTATGGTAACAAGCTGCCAGCTCATCGTTGCTAA
mm4_intergenic_Gm10040|Gm17641/1110032F04Rik_chr3_68818434 GGTTCAACCCAGGGCTGTAGCCACCGTGCCTCTCCGCTCACCTCACCCGACTCTCCTCAGGGTGGAAGCATGCTTCAGTC
CCTCTTTGAATTTTACACCTCTGCCAGTTTTGAAGGTCACAGGTGCCA
mm4_intergenic_4933405L10Rik|Gfod2_chr8_105710958 CTGTAGCAGGTGAGCCAGACTTGGGCCTGACTTTGCAGAGGGCTGGAGAGACGGCTTTGGTTAGGGAT
mm4_intron_Papss1_chr3_131614055 TACACTGTGATTGCCCCGTGCTGTGTGCCCTCTGTCTTTGACAAAAGGGATGATTTTATGATCCTGGGACAACTCACCCC
CTCTGGAAAGCAGGG
ATGCCACTACACTCCTCTTGGC
mm4_intron_Nxnl2_chr13_51172982 AGTTGAGATGGTGCTGGTGGCACTAATTAGGCGGAGAACTCTGCCCCTCTGGAGACTAGGGCGCTGGGTTGCTGGCGAGG
ATGGTAG
mm3_intron_Fbxo21_chr5_118003644 TTCCTCATTGCCCAGTGGTCACAGGGCAGCCCTGCACAGTGTGACAGGCTCAGCCACGTAGTTCCGCTATGGCTCCCTCC
CTCTGCAGAGCCTGG
CATTATAGCACAGCTTCTCTCTTCAATCTCTGCTGCACCAGGAC
mm4_intergenic_Ucn3|Gm22460_chr13_3947836 CTCTCTTGAAGCCAGACCCCCTCCCCCTCTGTGAAGATTCAGGCATCTTTGTGACTGTTGTTGTCTCTGGAACCTAGGGT
TTTGACAATGAGAACAAGCATATCAGTTTCCATTGGCTGACAGGACT
mm4_intron_Myh3_chr11_67095861 GGCTGCGTAGGGTTTACAGTAGTATTTTACTAGGCTGCTATTTGCTTTTCAGGGATACACCCCTACTCTCAAGAGGAGGG
GGG
TCTCTCACCTTTATTATTTGCAACCCCATTTGGCCCAATG
mm4_intergenic_mmu-mir-6393|Zdhhc25_chr15_87884816 CAGGAGAGTCAGAGGAGCCTACATTTTGTTCTCCCTCCCCATGGAGAGCCAGGAGTTAAAATTGACGCTATAATACCCAC
ATAGGATCTCTCTTAGTTTTAGCTCCACCAAGTAAACAGCTGAGAAGCCATTATGCCA
mm4_intergenic_Lep|Gm25589_chr6_29104937 GATTTTCAGCAAGCCCAAAGCTTCTCCAGACTCCGGAGAGGGGGGTAGGGGGAGGGCAGTTCCTTTTCTCTCCCATGTAC
ATTTAATTTCCCATTTTCCAGTTGCTCCTAGACAGATGAGTGGGGAGGAGGA
mm4_intergenic_n-R5s220|Cacna1e_chr1_154382958 TCCCTTGCTTCCCAAATGTTACTTACTCAGTAGCTTCTCCCCTACTCTGGAGAACCTGGACTATCTTCCAGGTAGAGATA
TGACCCTTGCACCTTACACT
mm4_intron_Parva_chr7_112557589 CCTAGCTCTCACACCCTCCTCCTCCCTCCCTCCCAACCTCTGCAGAGACCGGAGTCGGTCACCAGCAGTCTAG
mm4_intron_Map3k4_chr17_12235355 TGAGTGCTGAGGGAGTGGTACAGCCATGCCCGACTCTCCTCAGGGGGGCTGCCTGCCTCCTGTCTGGGATGGTACAGC
mm4_intron_Stmn2_chr3_8551222 GTAGGGTCAGAACTGGGTGCAGTTTACACAGTCGCCCACCCTCTCGAGAGGCTGGATCTCCGCCGTTATGGGGAGGAGCC
TCTACTTC
mm4_intron_Kcnh1_chr1_192445287 TGGCTGTTCTAGGTGCACAGGGTCACAGTATGGTAGGGCCTAAGCAGCATCCGGGCTATCATGGCTGGGTTCTGGCCACT
ACTGCCTCCCTCTGTGGAGTCAGGCACTGTCTGTTCCTGTGCGTGCTTACCCCTCATTT
mm4_intergenic_Gm16155|Tmcc3_chr10_94426566 GGTGAGAGATGCAGGCCAATCAACAGTTTCCGCACAGAGTGGGAGGGGCCGTGGTGGGGCAGCTGCAGGGCAAGGCGGCT
TCCTGACTCTAGAGAGGTGGGGACACACAAGAGTTAACCCGCTGAATAGGGACAGA
mm4_intergenic_Gm5218|Gm23880_chr15_81523282 CTCCGCTGCAAGCCATTCTGCGCACTCGCTCCCTCTGGGGAGGCTGGTCACGTGCCCGGGGTGGGGGTTGGGAGGACGAG
CAGCAAGCGCCCCCGGGCTGCACGTGCCGGGCTAGATGAGGGCGGAGCTCC
mm4_intergenic_Erv3|Lamr1-ps1_chr2_131892620 TGGTGTGCAAAACCCAGACTTCTGTAAACCAGACTCACTAGAGGAGGGCAGACAGCAAAGCGAAGAAACCTCAGACTTAG
GAGAGGATGCAGAAACCGGAGAAAGGAGCCA
mm4_intergenic_Gm12462|Pax5_chr4_44677301 TGCCTGCCACTCCTTTTCATACCCACCGAGGGCTCTGCCCAGGCGCAGGCTGCTTCATCCCTACTCCTCCTCTCTGGAGG
ATCAGG
CAGGTGACAAGCATTCAGATGATGCCAGGGGATGAGG
mm4_exon_Dnah2_chr11_69496625 AGATGGTGGCAAAGAGGTGGCTGCCTATGTCATTGACCACACTCGCCAGAGTCTGCAGAGTCGGGGAGAATTCCACCTGC
AGGAGAGAGCAGAGGAGCTCAGCACAGCTGGCTGGAGACACAAGCCTCGAG
mm4_intergenic_Gm20628/Hist2h3b/Hist2h2bb|Hist2h2bb_chr3_96276238 CCTGCTCATTCGTCGGGAATAACAATAAGATAGACCACTGTGTGCCAGACTCTCCACAGGTGAGCAGGGGATGGTATGTG
CTGAGTCTGAACTGAAGAAAGGCCAGTGTGGTGAATACC
mm4_intergenic_Zcchc6|Gas1_chr13_60014358 GTGGGCTTTGCGAAAACTCCATGCCCAGGCTCTCCAGGGGGCGAGAGACTTGGACACTGCTTTCTCCCTAGAAGGCTTCA
GAGTCAGACTGATGGCTCCAACCC
mm4_intron_Gm16168_chr17_10564418 AGCAAACCTAGTGACCAGCATTCCAAAACACCATCTCTGGAAGCCAGTGCTCCTCATCTCTGGAGTGTCTGGGCTCAATG
TCCTGTTAGTGTCTGTTCATGTTTTAGGTGTTAACTTCGGCCTGTTCTGACATCTG
mm4_intron_Lep_chr6_29070426 AAGAGTCATCAGGGCTGCACATGGAGAGGCCCAACTACCCAAATGTGGGTGGAAATGAGAGGAAGCCAGTGGGAAAAGCC
CTTCCTGGTAACCAGACTCAGCAGAGTGGGGGGGGGGGGCACGGCTTTGACCCTAATG
mm4_intergenic_5S_rRNA|Rasa2_chr9_96526758 CTCCCTGGGTTCCTTAGGGAATCAGCTGTCGTGGTATAGTCACATCCACACAGCATGAACCACCCAAACTCTCCAGCCGG
GGGCAG
GGTGTGTGAAAGAGGAGCAGATCTTGGGAGGT
mm4_intron_Ext2_chr2_93797405 GGATCGCTCTTCCTGTCCAGACAAAGGTTACTATAAACACCGACTGCATCTCCACCACTACCAGCCAGAAACTCCAGAGG
GAGGCAG
TACTTCCTGAGAGAGTATTTTAACAACTTCAGGGTGGGCC
mm4_intron_Magi1_chr6_93929116 ACGCTAGCTGACCCAGTTTCTGTGTTTGCTCCCCGACTCGCCACAGTGTGGGAGCCACAGACTTGGAAGTATCCCA
mm4_intergenic_Fars2|Gm26395_chr13_36555097 TGTGGCTTCTGTCCAGTGTCCTTAGCTCCTCCCACCCTCTGGAGACCTGGGTATATATGGAAGGGTGGAAAAGAGATGAA
TTTGCCTCTGTTGCAGCAGCTGCAGATTGGTCCCTACA
mm4_intergenic_Bmper|Npsr1_chr9_23697125 TACAAACTCCCCTGCACCACGTGTGGGAAGTCTCCACTCCTGTGGAGAGACTGGTGTTCTTCATTGTTCTAATTAAAGGA
CAATCTGATGCTGGAGGTGCC
mm4_exon_Amz1_chr5_140752245 CGCCATGGAGGAGTATGGACAGTGGCTGACTGCGTGCATCCAGACCCTGGAGAGGGAGGGAGCTGAAGAGGAGCTGCTCC
AGGTGGATGCGGCTGTGGATGCCCTGAGCCGGTGGGAGATGTTCACAGGG
mm4_intergenic_Rbm33|Shh/9530036O11Rik_chr5_28456604 AGAGTTGGAAATGGGCAGGGGAAGATTGTCCCCCTCAATTCCCCAACTCTGGAGAGTGTGGGCGGAGCTTTGCATAGCAA
GTACAGGGCTGGGGTCTTCCAATGAGGAGGGC
mm3_intergenic_5430435G22Rik|Slc26a9_chr1_131742805 GGGCTCCAAGACAGTGTTCACTGACACGTTCTCCCCCCCACTGGAGTGGCAGGCATACTTGGGTCCCTGGCATCCTGGCT
ACACCTCGAC
mm4_intron_Zfyve27_chr19_42174795 GTCTGAGGCTATGCGGAGAGCAGTGTGTCGCTGGATGGAGGGGGAGCTCCCCCCAGCTGGAGTCTCTGGATGCTGGCAGA
GGGATCACTGGTCTG
mm4_intergenic_Gfra2|Gm9192_chr14_71740223 GGTTCGCACAAGACATGTCACATGCTGTTGTGACTTCCCTCCTCTGAAGAGTGAGGGTGATGAATTCGGGAGTGGTGAAT
AACTTGATAAGTACCTAGAGGCTTGTACCCAGCATGGTGTGCCCACTCCAGATGCTTTT
mm4_exon_Rtn2_chr7_19287094 CTGGAGGAACTGAGGCTGCGGCTGGACCAGCTTGGCTGGGTTGTCCGGAGTGCGGGATCTGGGGAGGATTCGGCCACCAG
CAGCTCCACCCCTCTGGAAAATGAGGAGCCTGATGGATTGGAGGCCAG
mm4_intergenic_Ppargc1b|Gm25301_chr18_61397595 CTAAGCCTGGGTTAGCCACCAAAGACAAGAAGAACTCTTTGGGGGAACAGAGACAATCCACTTTCCCCAACCCTCCCGAG
GGGGAGAG
CTGGGGGGAGGGGAGGGTCTGGGAAGGGCTGTGGGGAAGAAGCTGGAC
mm4_intergenic_Prdx6|Tnfsf4_chr1_161288470 TCTCTGGCTGTGCTCTCTCTTTGCCCCAGTCGCTGCCTTGTAATCCTGACCCGCCAGAGGGGGCGGGGCCTCGCGGAGGC
ACGCCCCCCACGTCACAGGCTCACTCCCAAT
mm4_intron_Celsr1_chr15_85942654 AAACGCAGGGTAGTGTCACTAGATTTTACAACTGGCACACTAGATAGAAGACTCTTCCTCCTCCCTGGAGAGTCTGACTA
CAGTTCTCCTCAACACCCC
mm4_intergenic_Gm17118|Cacna2d2_chr9_107460850 CCAGCTAAGTCTGCCAGTCCTCTGCCCCCTCCCCTCTGGAGTGGCTGGCTTCCCCCTGCCCCAGCTCTTCAGTATCATCA
CCATCTTGTCTGCCCCAAGCTC
mm4_intron_Sfxn5_chr6_85220072 GAGGGAGCTGGATGTGTACGCACAGCCAGCGCCTCCAGAGTGGGGGAGCTTGCCCGGAAGAGCAGATGGAGGGGAACAGG
CACATTCGAGGGGCGGGGAAGGGAATGAGCCCACGAAG
mm3_intron_Ano10_chr9_122265208 TGGCTACCACTTTCACACCCTGATGGGATCTCAGTTAGCTGGAGGAAAAGAGAACTCCTCACCCATGAGTCCTCTTACAA
TCCATCACTCAGACTCTCCACAGAGGAGGAGGGAAGTAAAGGGCAAGCTACTGTGAAGG
mm4_intergenic_Rassf5|Ikbke_chr1_131251285 CTCTCTGGCCACTCTCGTTCCTCTCCATCTCTCANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
TGGTGAGGGAGG
TATGGAAAAACCAAGGCTCTAAAAGGCAGT
mm4_exon_Nhlrc4/Pigq_chr17_25944526 CACAGGTGTCTGGGTGTCTGCAGCAGGGGTCTGAGAGACACCACACACTCAAGAGGGGCGGAGGTTTACCTCTAAGGCTC
AAATCGCATGCAGAG
mm4_intron_Ssbp4_chr8_70604885 GCATCAGCATGTGCCATCAGTGCAGGATGGTGGCGGGGCCAGGGTGGCATCCCAGCCCTGCCCCCCTCCTGACAGTCAGG
GGCTGGGGTCCTCCTGGCATTTTCA
mm4_intron_Mrc2_chr11_105331462 TATGGGCCCTGCAATGTTCCTGGCTGCTCTCCCCACCTCTGGTCAGACTGGGCCTCACTATCAGCCAAGACTCCTTTCCC
TTGTTTCTTTTATCCAAGACTGCAGAGGTCAACACAGAGGG
mm4_intron_Mgll_chr6_88769972 GCAGCTGCTCCTATGACTCACTGGAAACCCCTTGGCCCTCCACACAGGCATCCTGGCCAGCCAGACTGTACAGAGTGGGT
GAG
AAGGGCCTATGGAGAGCGGACAGGAATCTACTGCCC
mm4_intron_L3mbtl3_chr10_26305159 AGCAAGATGAAAGACACCCAAAATACACTCACTATACAAATGACAAAGCCCTTTCTTTCTTACTAAGCCCAGTCTATCCA
GAAGGGGTGAG
AACTGCATCAGAAAGACAGCAAAGAGATGCAATGGCTGGTCATCGA
mm4_intergenic_Tha1|Gm11725_chr11_117879894 GCTGGATGAGTCTTGCGTCATCAGACTTACTCCGGCCCTCTGGGCAGTCAGGCACGTGGGATGTGGGATGTGTGGCCTTG
GGATGTGTAGCACCTTGCTTTGTGTCTGG
mm4_intergenic_Oaf|D630033O11Rik_chr9_43255585 TAGGAGGGGAGCAGAAGACCACCTGCTCCCCCCCTCAGGCCAGGCAGGCCTACAGATGAGACTCAGAGGGACCACCCAGG
GTGTGAGGCCTGCCTGCCACCAGTCCTGAGACCCAAGGGCCTGCAGGAGCTTCTGTGAA
mm3_intron_Csmd2_chr4_128299766 AGTGCAATTTCTACACCAATTGTGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNCTCCTCCCCTCTGGATAGTTTGGGTGGCCCTCACTGTTACTATT

Input file for Crispresso

Crispresso, written by Luca Pinello, is a software package to quantify the Cas9-induced mutations on off- or on-targets.

Click here to download an amplicon input file for Crispresso. For each off-target, it includes the off-target name, its PCR amplicon and the guide sequence. Keep a copy of this file.

After sequencing, run CRISPRessoPooled. The tool will map the reads to the amplicons and analyse the mutations:
CRISPRessoPooled -r1 Reads1.fastq.gz -r2 Reads2.fastq.gz -f crisporAmplicons_mN1FkRligAThyqKv3eg3.txt --name MY_EXPERIMENT

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CRISPR/Cas9 Guide Designer for chordate vertebrate ecdysozoans lophotrochozoans protostomes spongi corals plants butterflies metazoans genomes fruitflies insects nematodes mammals.