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Contents:In the table below, Illumina Nextera Handle sequences have been added and highlighted in bold. Primers for the on-target have been added for convenience. The table below is sorted by the CFD off-target score. Sites with very low CFD scores < 0.02 are unlikely to be cleaved, see our study Haeussler et al. 2016, Figure 2.
In the protocol by Matthew Canver, Harvard, two PCRs are run: one PCR to amplify the potential off-target, then a second PCR to extend the handles with Illumina barcodes. Please click here to download the protocol. Alternatively, you can have a look at Fu et al, 2014.
If a primer was not found, the reason is usually that the region around the off-target is too repetitive. To avoid unspecific primers, all repeats are masked for the primer design (not for off-target search). If you think that we should change the parameters here or should use different primer3 settings, please let us know.
| Name | Primer Sequence | Tm | CFD Score |
|---|---|---|---|
| ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52314112_F | TCGTCGGCAGCGTCGTCGATGGACTAGCAGGCAG | 60.2 | 1.00 |
| ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52314112_R | GTCTCGTGGGCTCGGAGCTACTAGGTTGCCCTTGG | 59.0 | 1.00 |
| mm4_intergenic_Zcchc6|Gas1_chr13_59975460_F | TCGTCGGCAGCGTCTTCCCTTCTGCCTTCTCGTG | 59.6 | 0.76 |
| mm4_intergenic_Zcchc6|Gas1_chr13_59975460_R | GTCTCGTGGGCTCGGACCAAAGCTACTGGGTGCTC | 59.9 | 0.76 |
| mm3_intron_9530036O11Rik_chr5_28842556_F | TCGTCGGCAGCGTCACCACCAGTGAATGCCCTTT | 59.8 | 0.69 |
| mm3_intron_9530036O11Rik_chr5_28842556_R | GTCTCGTGGGCTCGGGTGATACCAGGGATTCTCTGGG | 59.6 | 0.69 |
| mm4_intergenic_Gm22689|Gm9742_chr13_7531925_F | Primer3: not found at this Tm | N.d. | 0.68 |
| mm4_intergenic_Gm22689|Gm9742_chr13_7531925_R | Primer3: not found at this Tm | N.d. | 0.68 |
| mm4_intron_Cpeb3_chr19_37111797_F | Primer3: not found at this Tm | N.d. | 0.56 |
| mm4_intron_Cpeb3_chr19_37111797_R | Primer3: not found at this Tm | N.d. | 0.56 |
| mm4_intron_Nek6_chr2_38545570_F | Primer3: not found at this Tm | N.d. | 0.53 |
| mm4_intron_Nek6_chr2_38545570_R | Primer3: not found at this Tm | N.d. | 0.53 |
| mm3_exon_Rassf10_chr7_112954652_F | TCGTCGGCAGCGTCGTAGTGCTCAGCCGCGAG | 60.5 | 0.51 |
| mm3_exon_Rassf10_chr7_112954652_R | GTCTCGTGGGCTCGGGGTTGAGCTTGGCCAGTTTG | 59.9 | 0.51 |
| mm4_intergenic_Gm11691|Gm11692_chr11_114351503_F | Primer3: not found at this Tm | N.d. | 0.50 |
| mm4_intergenic_Gm11691|Gm11692_chr11_114351503_R | Primer3: not found at this Tm | N.d. | 0.50 |
| mm4_intron_Mov10_chr3_104799796_F | Primer3: not found at this Tm | N.d. | 0.48 |
| mm4_intron_Mov10_chr3_104799796_R | Primer3: not found at this Tm | N.d. | 0.48 |
| mm4_intergenic_Adam10|Lipc_chr9_70788235_F | Primer3: not found at this Tm | N.d. | 0.46 |
| mm4_intergenic_Adam10|Lipc_chr9_70788235_R | Primer3: not found at this Tm | N.d. | 0.46 |
| mm4_intergenic_Sec61b|NAMA_1_chr4_47588859_F | TCGTCGGCAGCGTCCGTGTTTTCCCTTGCCCAAA | 59.5 | 0.46 |
| mm4_intergenic_Sec61b|NAMA_1_chr4_47588859_R | GTCTCGTGGGCTCGGCCAAAGGAGCCCTTGGAGAT | 59.3 | 0.46 |
| mm4_intergenic_Neurod2|Ppp1r1b_chr11_98347135_F | TCGTCGGCAGCGTCTTGACCTAAAGGCTGCCCAG | 59.9 | 0.45 |
| mm4_intergenic_Neurod2|Ppp1r1b_chr11_98347135_R | GTCTCGTGGGCTCGGCCTGGACATGGGCATCTCTG | 60.1 | 0.45 |
| mm4_intron_Enah_chr1_181943177_F | Primer3: not found at this Tm | N.d. | 0.37 |
| mm4_intron_Enah_chr1_181943177_R | Primer3: not found at this Tm | N.d. | 0.37 |
| mm3_intergenic_Gm14661/Gm14651|Gm14661_chrX_60625025_F | Primer3: not found at this Tm | N.d. | 0.34 |
| mm3_intergenic_Gm14661/Gm14651|Gm14661_chrX_60625025_R | Primer3: not found at this Tm | N.d. | 0.34 |
| mm3_exon_Slc7a10/Lrp3_chr7_35202214_F | Primer3: not found at this Tm | N.d. | 0.31 |
| mm3_exon_Slc7a10/Lrp3_chr7_35202214_R | Primer3: not found at this Tm | N.d. | 0.31 |
| mm4_intergenic_Lrrn2|Mdm4_chr1_132954277_F | Primer3: not found at this Tm | N.d. | 0.29 |
| mm4_intergenic_Lrrn2|Mdm4_chr1_132954277_R | Primer3: not found at this Tm | N.d. | 0.29 |
| mm4_intron_Sh2d2a_chr3_87850477_F | TCGTCGGCAGCGTCGGCAGGAGATGAAGATGGCT | 59.5 | 0.27 |
| mm4_intron_Sh2d2a_chr3_87850477_R | GTCTCGTGGGCTCGGACCATCAACAGCTGGCTGAA | 59.8 | 0.27 |
| mm4_intron_Syt16_chr12_74265937_F | Primer3: not found at this Tm | N.d. | 0.27 |
| mm4_intron_Syt16_chr12_74265937_R | Primer3: not found at this Tm | N.d. | 0.27 |
| mm4_intron_Gm7976_chr16_95017274_F | Primer3: not found at this Tm | N.d. | 0.26 |
| mm4_intron_Gm7976_chr16_95017274_R | Primer3: not found at this Tm | N.d. | 0.26 |
| mm4_exon_Nol9_chr4_152039335_F | TCGTCGGCAGCGTCGTCTCGCGAGATCGTCCC | 59.6 | 0.24 |
| mm4_exon_Nol9_chr4_152039335_R | GTCTCGTGGGCTCGGGTGTAACACCTCCGACTCCG | 60.1 | 0.24 |
| mm4_intergenic_Umodl1|Abcg1_chr17_31038288_F | Primer3: not found at this Tm | N.d. | 0.24 |
| mm4_intergenic_Umodl1|Abcg1_chr17_31038288_R | Primer3: not found at this Tm | N.d. | 0.24 |
| mm4_intergenic_Prr22|Rfx2_chr17_56773852_F | Primer3: not found at this Tm | N.d. | 0.23 |
| mm4_intergenic_Prr22|Rfx2_chr17_56773852_R | Primer3: not found at this Tm | N.d. | 0.23 |
| mm4_intron_4930552P12Rik_chr19_55697662_F | Primer3: not found at this Tm | N.d. | 0.20 |
| mm4_intron_4930552P12Rik_chr19_55697662_R | Primer3: not found at this Tm | N.d. | 0.20 |
| mm3_intergenic_Irs1/Gm9747|Irs1_chr1_82283575_F | TCGTCGGCAGCGTCCGGGAGGGGTCTTGGAAGA | 60.6 | 0.20 |
| mm3_intergenic_Irs1/Gm9747|Irs1_chr1_82283575_R | GTCTCGTGGGCTCGGTTTTCCTGGGTTCACCTCCG | 59.8 | 0.20 |
| mm4_intron_Sdk2_chr11_113950528_F | TCGTCGGCAGCGTCGCCTCATCTTTCAGGGCTGT | 60.0 | 0.20 |
| mm4_intron_Sdk2_chr11_113950528_R | GTCTCGTGGGCTCGGTTGGCCAATTAGGAGCCTGG | 60.0 | 0.20 |
| mm4_intergenic_Gm23708|G6pd2_chr5_61506899_F | Primer3: not found at this Tm | N.d. | 0.19 |
| mm4_intergenic_Gm23708|G6pd2_chr5_61506899_R | Primer3: not found at this Tm | N.d. | 0.19 |
| mm4_intergenic_Mir187|2700062C07Rik_chr18_24453458_F | TCGTCGGCAGCGTCAGAACTGGAGCCTCCACTGA | 60.1 | 0.19 |
| mm4_intergenic_Mir187|2700062C07Rik_chr18_24453458_R | GTCTCGTGGGCTCGGGTTCTAGGGCGAGGGGTTTC | 60.1 | 0.19 |
| mm4_intergenic_Efnb1|Gm14812_chrX_99397677_F | Primer3: not found at this Tm | N.d. | 0.18 |
| mm4_intergenic_Efnb1|Gm14812_chrX_99397677_R | Primer3: not found at this Tm | N.d. | 0.18 |
| mm4_intergenic_Snx6|Cfl2_chr12_54814605_F | Primer3: not found at this Tm | N.d. | 0.17 |
| mm4_intergenic_Snx6|Cfl2_chr12_54814605_R | Primer3: not found at this Tm | N.d. | 0.17 |
| mm3_intron_Nrxn2_chr19_6510591_F | TCGTCGGCAGCGTCCTTGGCCTGAGAACCCAGAG | 60.0 | 0.16 |
| mm3_intron_Nrxn2_chr19_6510591_R | GTCTCGTGGGCTCGGTCCAAGAGCCTCATCCTCCT | 59.6 | 0.16 |
| mm4_intron_Ubxn11_chr4_134103095_F | Primer3: not found at this Tm | N.d. | 0.15 |
| mm4_intron_Ubxn11_chr4_134103095_R | Primer3: not found at this Tm | N.d. | 0.15 |
| mm4_intron_Phgdh_chr3_98315289_F | TCGTCGGCAGCGTCACAGGCACTTAACCCTCAGC | 59.9 | 0.13 |
| mm4_intron_Phgdh_chr3_98315289_R | GTCTCGTGGGCTCGGATCTGTCCCAGCTGGCTTTC | 60.0 | 0.13 |
| mm4_intron_Caprin2_chr6_148853424_F | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_intron_Caprin2_chr6_148853424_R | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_intergenic_Kbtbd8|AY512915_chr6_95150693_F | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_intergenic_Kbtbd8|AY512915_chr6_95150693_R | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_intergenic_Gm22718|Gm24213_chr6_19245344_F | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_intergenic_Gm22718|Gm24213_chr6_19245344_R | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_intron_Clec3b_chr9_123152378_F | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_intron_Clec3b_chr9_123152378_R | Primer3: not found at this Tm | N.d. | 0.13 |
| mm3_intron_Snx15_chr19_6127816_F | Primer3: not found at this Tm | N.d. | 0.12 |
| mm3_intron_Snx15_chr19_6127816_R | Primer3: not found at this Tm | N.d. | 0.12 |
| mm4_intron_Cers6_chr2_69063463_F | Primer3: not found at this Tm | N.d. | 0.12 |
| mm4_intron_Cers6_chr2_69063463_R | Primer3: not found at this Tm | N.d. | 0.12 |
| mm4_intergenic_Gm6811|Gm7732_chr17_21098321_F | Primer3: not found at this Tm | N.d. | 0.11 |
| mm4_intergenic_Gm6811|Gm7732_chr17_21098321_R | Primer3: not found at this Tm | N.d. | 0.11 |
| mm4_intergenic_Eln|Wbscr28_chr5_134751846_F | TCGTCGGCAGCGTCCCAGTTCAACCTCCCTAGCC | 59.7 | 0.11 |
| mm4_intergenic_Eln|Wbscr28_chr5_134751846_R | GTCTCGTGGGCTCGGAAGACAGGCCAGGAGCTAGA | 59.9 | 0.11 |
| mm4_intron_Fbln1_chr15_85210527_F | TCGTCGGCAGCGTCGCATGCCACATACCTCCTCA | 59.8 | 0.11 |
| mm4_intron_Fbln1_chr15_85210527_R | GTCTCGTGGGCTCGGGTAAGATATGAGGCCACCGGG | 59.9 | 0.11 |
| mm4_intergenic_Gm7168|Dact2_chr17_13996137_F | TCGTCGGCAGCGTCCCAGAAAACTGTGCCAGAAGC | 60.0 | 0.10 |
| mm4_intergenic_Gm7168|Dact2_chr17_13996137_R | GTCTCGTGGGCTCGGTGCTTGCACAGGGTTACCAG | 60.5 | 0.10 |
| mm4_intergenic_Dpysl5|Mapre3_chr5_30806406_F | TCGTCGGCAGCGTCCACAGGCCACCATGAAGTGA | 60.2 | 0.10 |
| mm4_intergenic_Dpysl5|Mapre3_chr5_30806406_R | GTCTCGTGGGCTCGGCATGTGCTGGTTGCTTGCTT | 59.9 | 0.10 |
| mm4_exon_Sohlh2_chr3_55207808_F | TCGTCGGCAGCGTCAGCTGCCAGGACAAATCAGA | 59.3 | 0.10 |
| mm4_exon_Sohlh2_chr3_55207808_R | GTCTCGTGGGCTCGGCACTTACCAGGCAGTTGGGA | 59.6 | 0.10 |
| mm4_exon_Uchl1os_chr5_66626923_F | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_exon_Uchl1os_chr5_66626923_R | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intergenic_Gm7618|Fam60a_chr6_148906161_F | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intergenic_Gm7618|Fam60a_chr6_148906161_R | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intron_Osbpl9_chr4_109187815_F | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intron_Osbpl9_chr4_109187815_R | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_exon_Bag1_chr4_40948083_F | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_exon_Bag1_chr4_40948083_R | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_exon_Gmeb2_chr2_181253917_F | TCGTCGGCAGCGTCTCCAGTGGCAGTTGCTGTAG | 59.9 | 0.08 |
| mm4_exon_Gmeb2_chr2_181253917_R | GTCTCGTGGGCTCGGTGGCAGCACAGTACTGAAGG | 59.9 | 0.08 |
| mm4_intron_Fgfrl1_chr5_108700144_F | TCGTCGGCAGCGTCTCAGAGGTATGGATCTGGCTGA | 60.0 | 0.08 |
| mm4_intron_Fgfrl1_chr5_108700144_R | GTCTCGTGGGCTCGGGCTGGCTCCTTCAGGAATGA | 59.7 | 0.08 |
| mm4_intergenic_Gm4825|Wnt7b_chr15_85515500_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_Gm4825|Wnt7b_chr15_85515500_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_Tacc1|Gm10689_chr8_25347523_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_Tacc1|Gm10689_chr8_25347523_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intron_Dock6_chr9_21804648_F | TCGTCGGCAGCGTCGCCAGAGCTCTGTGGGAAAA | 60.2 | 0.07 |
| mm4_intron_Dock6_chr9_21804648_R | GTCTCGTGGGCTCGGAGATTATGCACCAGGTGGGC | 60.1 | 0.07 |
| mm4_intergenic_Sult1a1|Gm6939_chr7_126687122_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_Sult1a1|Gm6939_chr7_126687122_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_Gm8832|Frmpd4_chrX_168388389_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_Gm8832|Frmpd4_chrX_168388389_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_Cask|Gm5381_chrX_13898672_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_Cask|Gm5381_chrX_13898672_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_Rpl10-ps3|1600029D21Rik_chr9_50476784_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_Rpl10-ps3|1600029D21Rik_chr9_50476784_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_Tram2|Tmem14a_chr1_21162925_F | TCGTCGGCAGCGTCTCTCAATCCCCAGGCTCTGA | 59.9 | 0.06 |
| mm4_intergenic_Tram2|Tmem14a_chr1_21162925_R | GTCTCGTGGGCTCGGGGAGGCTAATCCGGAAACCC | 60.1 | 0.06 |
| mm4_intergenic_Psd4|Pax8_chr2_24413638_F | TCGTCGGCAGCGTCGCCACAAGCTGAGGTGTTTG | 59.9 | 0.06 |
| mm4_intergenic_Psd4|Pax8_chr2_24413638_R | GTCTCGTGGGCTCGGAGGCAGCTTATCCTCTCCCT | 59.7 | 0.06 |
| mm4_intron_Ttll13_chr7_80252977_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intron_Ttll13_chr7_80252977_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intron_Nek6_chr2_38568963_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intron_Nek6_chr2_38568963_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_Gm26784|1700018B08Rik_chr8_121481503_F | TCGTCGGCAGCGTCGCTAATAGTCCAGGGTGGCC | 59.8 | 0.05 |
| mm4_intergenic_Gm26784|1700018B08Rik_chr8_121481503_R | GTCTCGTGGGCTCGGTGTTTTCATCGAGGGAGGGC | 60.0 | 0.05 |
| mm4_intron_Cntn5_chr9_10089136_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intron_Cntn5_chr9_10089136_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm3_intergenic_Hdac4|Ndufa10_chr1_92282138_F | TCGTCGGCAGCGTCAGACTCGCCTCTGTCTCCTC | 60.3 | 0.05 |
| mm3_intergenic_Hdac4|Ndufa10_chr1_92282138_R | GTCTCGTGGGCTCGGTTCTCTGGGCCATAGCTTGC | 60.1 | 0.05 |
| mm4_exon_Zdhhc25_chr15_88601312_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_exon_Zdhhc25_chr15_88601312_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intron_Adcy2_chr13_68927753_F | TCGTCGGCAGCGTCGCTGCATTGCCAACCTACAT | 59.1 | 0.05 |
| mm4_intron_Adcy2_chr13_68927753_R | GTCTCGTGGGCTCGGCACTGTCACTGGGGTGTGAT | 59.6 | 0.05 |
| mm4_exon_Rnf139_chr15_58889269_F | TCGTCGGCAGCGTCCGGAGCCACTTTCGTCGG | 60.8 | 0.05 |
| mm4_exon_Rnf139_chr15_58889269_R | GTCTCGTGGGCTCGGCATCCGCACCTGCTGCTG | 61.5 | 0.05 |
| mm4_intergenic_Tjp1|Gm7546_chr7_65527815_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_Tjp1|Gm7546_chr7_65527815_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_exon_Erbb3_chr10_128589530_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_exon_Erbb3_chr10_128589530_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_Gm9725|Gm4899_chr8_81081563_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_Gm9725|Gm4899_chr8_81081563_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_exon_Mybbp1a/Spns2_chr11_72452981_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_exon_Mybbp1a/Spns2_chr11_72452981_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intron_Rgs3_chr4_62688165_F | TCGTCGGCAGCGTCGGATCCATCTCCTGGTTGGC | 60.1 | 0.04 |
| mm4_intron_Rgs3_chr4_62688165_R | GTCTCGTGGGCTCGGTATGAGATGACCTGGCCCCA | 60.0 | 0.04 |
| mm4_intron_Col17a1_chr19_47672305_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intron_Col17a1_chr19_47672305_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Zcchc2|Gm26509_chr1_106037851_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Zcchc2|Gm26509_chr1_106037851_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_exon_Tbc1d2_chr4_46649960_F | TCGTCGGCAGCGTCTAGGGACCGTGTCTAGAGGC | 60.1 | 0.04 |
| mm4_exon_Tbc1d2_chr4_46649960_R | GTCTCGTGGGCTCGGTCTTCCTCGGAGTCCACACA | 60.1 | 0.04 |
| mm4_intron_Dnajb13_chr7_100512747_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intron_Dnajb13_chr7_100512747_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intron_Rufy1_chr11_50393688_F | TCGTCGGCAGCGTCACCCTTTCAGCCAGTTCCAC | 60.1 | 0.03 |
| mm4_intron_Rufy1_chr11_50393688_R | GTCTCGTGGGCTCGGTTTCCCCACTCCATCCTCCT | 59.8 | 0.03 |
| mm4_intergenic_Gm23534|Gm22727_chr1_137390130_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Gm23534|Gm22727_chr1_137390130_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intron_Ap4e1_chr2_127051743_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intron_Ap4e1_chr2_127051743_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intron_Arntl2_chr6_146822961_F | TCGTCGGCAGCGTCGTCTGGCTTTGGTGGTGTCT | 60.1 | 0.02 |
| mm4_intron_Arntl2_chr6_146822961_R | GTCTCGTGGGCTCGGAGCCTGTACAACCAAAGCCT | 59.5 | 0.02 |
| mm4_intergenic_Bcar3|Gm25153_chr3_122464645_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Bcar3|Gm25153_chr3_122464645_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm3_intergenic_Ssbp4|Lrrc25_chr8_70609669_F | TCGTCGGCAGCGTCTTCCAGGCGCTCAGGAAAG | 60.0 | 0.02 |
| mm3_intergenic_Ssbp4|Lrrc25_chr8_70609669_R | GTCTCGTGGGCTCGGGGGGAGAGGAAGCTGGGG | 61.0 | 0.02 |
| mm4_intergenic_Gm26028|Gm23576_chr18_60005289_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Gm26028|Gm23576_chr18_60005289_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intron_Cep170b_chr12_112744239_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intron_Cep170b_chr12_112744239_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intron_Cep170b_chr12_112744219_F | TCGTCGGCAGCGTCAGGTGAGAGACAGAGACCCC | 59.9 | 0.02 |
| mm4_intron_Cep170b_chr12_112744219_R | GTCTCGTGGGCTCGGCAAGGATGAGGGTCCAGGC | 59.7 | 0.02 |
| mm3_exon_Atn1_chr6_124745028_F | TCGTCGGCAGCGTCCAGGCTCGGATGAAAAGGGA | 59.7 | 0.02 |
| mm3_exon_Atn1_chr6_124745028_R | GTCTCGTGGGCTCGGAGTGAGCTGGAACCCCTACA | 60.1 | 0.02 |
| mm4_intron_Itgae_chr11_73094969_F | TCGTCGGCAGCGTCCAGCCTGTGTCACAGTGTGA | 60.1 | 0.02 |
| mm4_intron_Itgae_chr11_73094969_R | GTCTCGTGGGCTCGGACTTCATTCTTTCCTCAGAGGCT | 59.4 | 0.02 |
| mm4_intron_Ptprr_chr10_116233498_F | TCGTCGGCAGCGTCTGCAGTGAAGTCCAAGATGGG | 60.2 | 0.02 |
| mm4_intron_Ptprr_chr10_116233498_R | GTCTCGTGGGCTCGGTGGCAAGATGGGGAAAGGTG | 60.2 | 0.02 |
| mm4_intron_Phactr4_chr4_132422252_F | TCGTCGGCAGCGTCGAGGGAGGAGGCTACCGC | 61.2 | 0.02 |
| mm4_intron_Phactr4_chr4_132422252_R | GTCTCGTGGGCTCGGAAAGTGAGCACCTCCCGC | 59.9 | 0.02 |
| mm3_intergenic_Mir5127|Gm17383_chr18_82234723_F | TCGTCGGCAGCGTCTGAGCCAACACAAACCACCT | 60.0 | 0.02 |
| mm3_intergenic_Mir5127|Gm17383_chr18_82234723_R | GTCTCGTGGGCTCGGGCTCTGCTCTGCTTCCCTAA | 59.4 | 0.02 |
| mm4_intron_Cass4_chr2_172421793_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intron_Cass4_chr2_172421793_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Nbas|Gm4929_chr12_13626953_F | TCGTCGGCAGCGTCAACCCTTTCTCATCTGCGCT | 59.6 | 0.02 |
| mm4_intergenic_Nbas|Gm4929_chr12_13626953_R | GTCTCGTGGGCTCGGCCCAACTCCACTCAGCTGTT | 59.8 | 0.02 |
| mm4_intron_Fam220a_chr5_143557183_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_Fam220a_chr5_143557183_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Gna13|9930022D16Rik_chr11_109406721_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Gna13|9930022D16Rik_chr11_109406721_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Gm10505|Grm4_chr17_27401949_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Gm10505|Grm4_chr17_27401949_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm3_intergenic_Nkx1-1|Fam53a_chr5_33578170_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm3_intergenic_Nkx1-1|Fam53a_chr5_33578170_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_exon_Oasl2_chr5_114911708_F | TCGTCGGCAGCGTCTCAAGTTTCTTTTCCTAACAGTCAA | 57.0 | 0.01 |
| mm4_exon_Oasl2_chr5_114911708_R | GTCTCGTGGGCTCGGAGACACAGGTGTGACTCAGC | 59.6 | 0.01 |
| mm4_intron_A330074K22Rik_chr8_120207963_F | TCGTCGGCAGCGTCAGAAGTCGCTAACCCAGTGC | 60.0 | 0.01 |
| mm4_intron_A330074K22Rik_chr8_120207963_R | GTCTCGTGGGCTCGGGAAAACCAGAGGACTGCCCA | 59.8 | 0.01 |
| mm4_intron_Col27a1_chr4_63257252_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_Col27a1_chr4_63257252_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_exon_Tbr1_chr2_61811741_F | TCGTCGGCAGCGTCTTCGTGAGCAACTACGCCAA | 60.2 | 0.01 |
| mm4_exon_Tbr1_chr2_61811741_R | GTCTCGTGGGCTCGGCAGCAGCCCGTTGGTGTG | 61.6 | 0.01 |
| mm4_intergenic_Gm5468|Myo10_chr15_25438647_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Gm5468|Myo10_chr15_25438647_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_Whamm_chr7_81572958_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_Whamm_chr7_81572958_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Rap2c|Gm5387_chrX_51019121_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Rap2c|Gm5387_chrX_51019121_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_Ssh1_chr5_113979443_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_Ssh1_chr5_113979443_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_Cep170b_chr12_112744319_F | TCGTCGGCAGCGTCGACCCTCATCCTTGAGCCTG | 59.8 | 0.01 |
| mm4_intron_Cep170b_chr12_112744319_R | GTCTCGTGGGCTCGGCCGAGGGTAGAGGTCCAGG | 60.4 | 0.01 |
| mm4_intron_Cep170b_chr12_112744299_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Cep170b_chr12_112744299_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm3_intron_Stk10_chr11_32614430_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm3_intron_Stk10_chr11_32614430_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Adamts2_chr11_50622773_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Adamts2_chr11_50622773_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Hhat_chr1_192762961_F | TCGTCGGCAGCGTCGGCAGCCCTCAAGTCTTCTT | 59.9 | 0.00 |
| mm4_intron_Hhat_chr1_192762961_R | GTCTCGTGGGCTCGGCCATTTACATGCTGTTCAAGGAG | 58.0 | 0.00 |
| mm4_intergenic_Gpr89|Gja8_chr3_96913468_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gpr89|Gja8_chr3_96913468_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_G630030J09Rik/Gramd1a|Scgb1b2_chr7_31180115_F | TCGTCGGCAGCGTCGGTGGCCAAGAAGGAGCTAG | 60.1 | 0.00 |
| mm4_intergenic_G630030J09Rik/Gramd1a|Scgb1b2_chr7_31180115_R | GTCTCGTGGGCTCGGAGCATGCAAAGTCAGCAGAA | 58.3 | 0.00 |
| mm4_intergenic_Gm14254|Tox2_chr2_163190204_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm14254|Tox2_chr2_163190204_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Chi3l4|BC051070_chr3_106333014_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Chi3l4|BC051070_chr3_106333014_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Polr1a|St3gal5_chr6_72069533_F | TCGTCGGCAGCGTCGCAAACAAACAGGAGGAGGC | 59.6 | 0.00 |
| mm4_intergenic_Polr1a|St3gal5_chr6_72069533_R | GTCTCGTGGGCTCGGACATATTCCCAGGCTACCCCA | 60.3 | 0.00 |
| mm4_intron_Zfhx3_chr8_108752978_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Zfhx3_chr8_108752978_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Sumo2_chr11_115535988_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Sumo2_chr11_115535988_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Snx10_chr6_51535951_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Snx10_chr6_51535951_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Rps6ka2_chr17_7299452_F | TCGTCGGCAGCGTCCAGAGTCCCGAGATTGTGCA | 59.7 | 0.00 |
| mm4_intron_Rps6ka2_chr17_7299452_R | GTCTCGTGGGCTCGGGTTTCTCCCTTCCACCACCC | 60.2 | 0.00 |
| mm4_intron_Mterfd1_chr13_66918887_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Mterfd1_chr13_66918887_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Kcnab2_chr4_152443516_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Kcnab2_chr4_152443516_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Katnal2_chr18_77021622_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Katnal2_chr18_77021622_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Homer2_chr7_81612210_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Homer2_chr7_81612210_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Gna11_chr10_81533436_F | TCGTCGGCAGCGTCCATTGGCCTGAGGGAGGAAG | 60.1 | 0.00 |
| mm4_intron_Gna11_chr10_81533436_R | GTCTCGTGGGCTCGGGCTTTCTAGAGGCCTTGGCT | 59.7 | 0.00 |
| mm4_intron_Eya4_chr10_23187720_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Eya4_chr10_23187720_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_B3galtl_chr5_149734529_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_B3galtl_chr5_149734529_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_n-R5s175|Gm2301_chr5_119436385_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_n-R5s175|Gm2301_chr5_119436385_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Rdh14|Pgk1-rs7_chr12_10768568_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Rdh14|Pgk1-rs7_chr12_10768568_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Prune2|Gcnt1_chr19_17243583_F | TCGTCGGCAGCGTCCACACCCGACTCTGCTTTCT | 59.9 | 0.00 |
| mm4_intergenic_Prune2|Gcnt1_chr19_17243583_R | GTCTCGTGGGCTCGGAGCAGGAGAAAGAACACGCA | 59.8 | 0.00 |
| mm4_intergenic_Me1|A330041J22Rik/Me1_chr9_86688098_F | TCGTCGGCAGCGTCCTGAGAGAGAAGGTGACGCC | 59.8 | 0.00 |
| mm4_intergenic_Me1|A330041J22Rik/Me1_chr9_86688098_R | GTCTCGTGGGCTCGGTGTTTCCTGCAGCTGACACA | 60.1 | 0.00 |
| mm4_intergenic_Gpr12|Usp12_chr5_146691896_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gpr12|Usp12_chr5_146691896_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm24880|Gm10172_chr7_71156417_F | TCGTCGGCAGCGTCGCAGAGCAAGCTGGACAATG | 59.8 | 0.00 |
| mm4_intergenic_Gm24880|Gm10172_chr7_71156417_R | GTCTCGTGGGCTCGGCAGGTGAAGTCGGAGCAGAG | 60.1 | 0.00 |
| mm4_intergenic_Gm24092|Cenpf_chr1_189541042_F | TCGTCGGCAGCGTCTGCACACTCATTCCAGGCTT | 59.8 | 0.00 |
| mm4_intergenic_Gm24092|Cenpf_chr1_189541042_R | GTCTCGTGGGCTCGGTGATCTGTCTGACTCCAGCG | 59.1 | 0.00 |
| mm4_intergenic_Gm15985|Gm6116_chr5_74877692_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm15985|Gm6116_chr5_74877692_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm10184|Nrxn1_chr17_90010836_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm10184|Nrxn1_chr17_90010836_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Cyp2c55/Gm23800|Cyp2c55_chr19_39009823_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Cyp2c55/Gm23800|Cyp2c55_chr19_39009823_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Cxcr4|Gm26686_chr1_128709104_F | TCGTCGGCAGCGTCACTCCCCTGGTAGGATGACC | 60.0 | 0.00 |
| mm4_intergenic_Cxcr4|Gm26686_chr1_128709104_R | GTCTCGTGGGCTCGGACAAGAGGCTGATGTGGTGT | 59.2 | 0.00 |
| mm4_intergenic_Btbd16|Gm5602_chr7_130829412_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Btbd16|Gm5602_chr7_130829412_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Apob|1110057K04Rik_chr12_8115888_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Apob|1110057K04Rik_chr12_8115888_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_6530403H02Rik|Rwdd3_chr3_120951249_F | TCGTCGGCAGCGTCGCCTAAACCCACAATTCTATGCA | 59.3 | 0.00 |
| mm4_intergenic_6530403H02Rik|Rwdd3_chr3_120951249_R | GTCTCGTGGGCTCGGGCGCTTGACTCAGAACAGGA | 60.3 | 0.00 |
| mm4_intergenic_4930505O20Rik|Frem3_chr8_80573258_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_4930505O20Rik|Frem3_chr8_80573258_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_4930448N21Rik|Stox2_chr8_47332309_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_4930448N21Rik|Stox2_chr8_47332309_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_exon_Arhgef40_chr14_51999840_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_exon_Arhgef40_chr14_51999840_R | Primer3: not found at this Tm | N.d. | 0.00 |
These only list off-targets that have primers in the table above. Primers underlined, off-targets in bold.
| ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52314112 |
GTCGATGGACTAGCAGGCAGTGCTGCAGGGCTGGGCGCCGAGCCACGGTCTGCTGGAGCGGCCATGCTTGGCCCGGGACC CCCAGTCCCCTCCGCGGACAGCCTCTCTGGCCAAGGGCAACCTAGTAGCT |
| mm4_intergenic_Zcchc6|Gas1_chr13_59975460 |
TTCCCTTCTGCCTTCTCGTGTATTCACAAGTCTGTGAAGTTCCTCTTCTGTTTGTTTCCCTCCAAACCCCTTGCTTGGCT CAGGATTCCCACGCTGGGAGTCTGAGAGCACCCAGTAGCTTTGGT |
| mm3_intron_9530036O11Rik_chr5_28842556 |
ACCACCAGTGAATGCCCTTTGTAGTCTTTGAGGTGCTGGCCAGGCATGGCCTGAGTGAGAGGAAGGAGGCTTGTTGAGTC CCTGCTTGACCTAGGACCCCCACCCAGAGAATCCCTGGTATCAC |
| mm3_exon_Rassf10_chr7_112954652 |
GTAGTGCTCAGCCGCGAGCGCCCCTGCTTGGCCCGGGGCGCCCCAGCACGGCCCAGCCTGGCCTTGACCCAGGAGAAGCA GCGGCGGGTGGTACGCAAGGCCTTCCGCAAACTGGCCAAGCTCAACC |
| mm4_intergenic_Sec61b|NAMA_1_chr4_47588859 |
CGTGTTTTCCCTTGCCCAAATCACTGTGTTTTTACCATCCCAATGACATCCCTGAATTGGAGTTCTTGCCTTGGGCTTTG CTTTCTGGAGGGCCTGAGCCAAGCATGGTTTGTATTCAGTATCTCCAAGGGCTCCTTTGG |
| mm4_intergenic_Neurod2|Ppp1r1b_chr11_98347135 |
TTGACCTAAAGGCTGCCCAGCGACTCTCTTGATCCTCCCAACATAACAACAGGCTGGGTTTTGCCATTCCTCACCACACC CCATCTGGAAGAGGGGTCCTGGGCCAAGCTAGGAACTGGCAGAGATGCCCATGTCCAGG |
| mm4_intron_Sh2d2a_chr3_87850477 |
GGCAGGAGATGAAGATGGCTACAATGTTTCAGAAGGTGATGGGCGGCCAGGGCCAAACAAGGCCACTAGAGATGAAGACA GCTGCTATGTCAGTGTTTCAGCCAGCTGTTGATGGT |
| mm4_exon_Nol9_chr4_152039335 | GTCTCGCGAGATCGTCCCGCCGGAGGGGTTCCGGGACAAGCATGGCGGAGTCGGAGGTGTTACAC |
| mm3_intergenic_Irs1/Gm9747|Irs1_chr1_82283575 | CGGGAGGGGTCTTGGAAGAGGGGGGTCCAGAGCCAAGCAAAGAGGGGGCAGGCAGGAAGCGGAGGTGAACCCAGGAAAA |
| mm4_intron_Sdk2_chr11_113950528 |
GCCTCATCTTTCAGGGCTGTCTACTTCCTAGCTTGGCCTGGGAACCACACAGCTCCCCTGGAGCCATTCCCGCTGGGAAT ACTGGCAGAGGGGAAACAGGGGTGAAGGCCTCTGCCAGGCTCCTAATTGGCCAA |
| mm4_intergenic_Mir187|2700062C07Rik_chr18_24453458 |
AGAACTGGAGCCTCCACTGAGTTTCTGTAAATGCCCCAGGCGGATGAACCCAGGGCTCCCTGGCCAAGCAGGGTAGACAA GCTAAGGAGACACAGGGAGGTAAAGAAACCCCTCGCCCTAGAAC |
| mm3_intron_Nrxn2_chr19_6510591 |
CTTGGCCTGAGAACCCAGAGCTGAGGACACAGAGAGCCTTAGTTCCTAGGGCTACAGCTGTGGGGGTCAAGGGCAAAGCA AGGTCTTGGGAGCCCAGGGGAGGGAGGAGGATGAGGCTCTTGGA |
| mm4_intron_Phgdh_chr3_98315289 |
ACAGGCACTTAACCCTCAGCTACAAGGCCCTTGTTGGCCTGGGAACCCCAACTGCACCCTGCACTGGGCACTGCGACTGC TTCCAAGTGCTGAAAGCCAGCTGGGACAGAT |
| mm4_intergenic_Eln|Wbscr28_chr5_134751846 |
CCAGTTCAACCTCCCTAGCCTTCCTTCCCTGACTGGCCTGGGATCCCCAGCCCCTAGGCTGCTCCTGAGGTTGGGATACT GGGCATTTGTTGGTGAGTCTCTAGCTCCTGGCCTGTCTT |
| mm4_intron_Fbln1_chr15_85210527 |
GCATGCCACATACCTCCTCACAGAGGACTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN GGTCCCAGGCCGAACAAGGATGCCCGGTGGCCTCATATCTTAC |
| mm4_intergenic_Gm7168|Dact2_chr17_13996137 |
CCAGAAAACTGTGCCAGAAGCAGGAGAGAAATACATGAATGTCAATCATAAGCCCCTTCTTGGCCCTGGCCCCACATTTC CAGGGTTGAGAACTGGTAACCCTGTGCAAGCA |
| mm4_intergenic_Dpysl5|Mapre3_chr5_30806406 |
CACAGGCCACCATGAAGTGACCAGGTAAGACTCAAGGAAAAACAACATGTTTCTATGCTTGGCCCTGAACCCCAGAATGG CTTAAAGCAAGCAACCAGCACATG |
| mm4_exon_Sohlh2_chr3_55207808 |
AGCTGCCAGGACAAATCAGAACATTTCCACTTATTTGCCACCCACTGCACCCAGTGTGTCCAACTTTATTCCTCAGCACT GCAATGCCATGCTTTGCCCAGCACGCCCAACCAGTCCCAACTGCCTGGTAAGTG |
| mm4_exon_Gmeb2_chr2_181253917 |
TCCAGTGGCAGTTGCTGTAGGCATTGTCACAATGGTGCTGCCTGCAGGTGATGCCTGAGCCACATTCTGTAGGGTGGGTC CCAGGCCAGGCAGGGTGAGCAGCTGCAAGGGGCTGACTACCTTCAGTACTGTGCTGCCA |
| mm4_intron_Fgfrl1_chr5_108700144 |
TCAGAGGTATGGATCTGGCTGATCTGGCTATAGAAGTTATAGAGAGACCAGAAGCTGGTATGGCTCCTTCCTTGGCCTGG GACCTTCAGATCATGTTGGGCTTTCTTAGACTCCTGTCATTCCTGAAGGAGCCAGC |
| mm4_intron_Dock6_chr9_21804648 |
GCCAGAGCTCTGTGGGAAAAGAGGAGGCAAGATTAGGGGTCCCAGGTCAAGGAGGGGCTGGAGAGGGCCTACCCGGGAAT GGGAGCAGCGGCCTAGGGCCCACCTGGTGCATAATCT |
| mm4_intergenic_Tram2|Tmem14a_chr1_21162925 |
TCTCAATCCCCAGGCTCTGAGCGACTTCTGGGGTTCCCAGGCAAAGAAAGGGAGCAGAGGGAGCCGGTTTCTGTAAGAGG GTTTCCGGATTAGCCTCC |
| mm4_intergenic_Psd4|Pax8_chr2_24413638 |
GCCACAAGCTGAGGTGTTTGAGGTTCCTGTCCCCACACCTTGGCCCTGTGGGGGTCTCCAGCCAGGCAGGGGCCTGACAG AGGGAGGGAGAGGATAAGCTGCCT |
| mm4_intergenic_Gm26784|1700018B08Rik_chr8_121481503 |
GCTAATAGTCCAGGGTGGCCCTGCCTGCCCTGCTTGTCACGGCAGCCCCAGAGAAACCATGCATTTTTATTCCATGAGAG GTTTCCAATACTACCAAGGATAGAGAGTGCCACCGCCCTCCCTCGATGAAAACA |
| mm3_intergenic_Hdac4|Ndufa10_chr1_92282138 |
AGACTCGCCTCTGTCTCCTCCCCTTTTACCCTTTAGAATACAACATTCCTGCTTGGCCTGGGACTTCCAAACACGTCTTC AGACACAAGATCTTAGCAAATCTTGCCAGGAGCAAGCTATGGCCCAGAGAA |
| mm4_intron_Adcy2_chr13_68927753 |
GCTGCATTGCCAACCTACATGTTTGCACTCACTTCACTTGCACATAATCTGTGATTGGGGATCCAGGGACAGGCATGGGA GTGGCATAGAATCACACCCCAGTGACAGTG |
| mm4_exon_Rnf139_chr15_58889269 |
CGGAGCCACTTTCGTCGGCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNAGCGGCTCATGGCGGCGGTGGGGCCCCCGCAGCAGCAGGTGCGGATG |
| mm4_intron_Rgs3_chr4_62688165 |
GGATCCATCTCCTGGTTGGCTCCAGGATTCTTCAGGAATAGAAGCTATGTGGAGTCACTGGTGCCCATCTTTGGCCTGGG ACCCACATCTTCAGAGTGTCTCCCCTGACTGGGGCCAGGTCATCTCATA |
| mm4_exon_Tbc1d2_chr4_46649960 |
TAGGGACCGTGTCTAGAGGCCGGGCGCAGCCCTCCGAATCCCCGGGAGATACCACCTCGGGCTCCCTGGCCAAGCCCGGA GGCTGTGTGGACTCCGAGGAAGA |
| mm4_intron_Rufy1_chr11_50393688 |
ACCCTTTCAGCCAGTTCCACGTCTGGTGAGTGCTCCTGGGCTGCCCAAGGAGTTCACCCGAGCTTGGCACGGGACAGCCA TGGATTTTGTGTTTGTTCAGGGAGAGGAGGATGGAGTGGGGAAA |
| mm4_intron_Arntl2_chr6_146822961 |
GTCTGGCTTTGGTGGTGTCTGGGTTTCGGCACAGCCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGAAGGCTTTGGTTGTACAGGCT |
| mm3_intergenic_Ssbp4|Lrrc25_chr8_70609669 |
TTCCAGGCGCTCAGGAAAGGCTGCCGGGGCGCGCCCCGCCTCCTCCCCCAGGGCTCAGCTGGGCCGACGCCGGCCTTTCC CATTTCCTGCTTGGCCCGCCACCCCCTCCCCCAGCTTCCTCTCCCC |
| mm4_intron_Cep170b_chr12_112744219 |
AGGTGAGAGACAGAGACCCCATCCTTGAGCCTGGCCCCCCATCCTCGGGCCTGGCCCCCCATCCTTGGGCCTGGCCCCCC ATCCTTGGGCCTGGCCCCCCATCCTCGGGCCTGGACCCTCATCCTTG |
| mm3_exon_Atn1_chr6_124745028 |
CAGGCTCGGATGAAAAGGGAAAGGATGCAGGCCAGGTGGCCCGGGCTGTAGAGCCAGGCCCCCGTGTCGGGGGAAGGGAT CCAGGCCTGGCCCGGGAACCCCATGTAGGGGTTCCAGCTCACT |
| mm4_intron_Itgae_chr11_73094969 |
CAGCCTGTGTCACAGTGTGAAAAGCAAAGGAATGGGGCTCCAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNAGCCTCTGAGGAAAGAATGAAGT |
| mm4_intron_Ptprr_chr10_116233498 |
TGCAGTGAAGTCCAAGATGGGTCATGAACACCTTGACTCTGTAACCAGTCCCGGCTTTGCCCAGGACCCACAGGAATACA CCTTTCCCCATCTTGCCA |
| mm4_intron_Phactr4_chr4_132422252 |
GAGGGAGGAGGCTACCGCCCGCCACGCCCTGGCTGCACTGACCGCCGCGGGCGCCGTTAACCCCCCACCCAGGCTCGGCC CGCGACCCCTAGTCGAGCCGAGCCTGCCGCTGCTGCGCGCGCGCGGGAGGTGCTCACTTT |
| mm3_intergenic_Mir5127|Gm17383_chr18_82234723 |
TGAGCCAACACAAACCACCTCCCTCTAGATGCCTAGGGTGGCTTCACTCCTTCAAGCTGGAGGTCCGGAGCCAAGCATGA CTATCTTAGGGAAGCAGAGCAGAGC |
| mm4_intergenic_Nbas|Gm4929_chr12_13626953 |
AACCCTTTCTCATCTGCGCTGTCTTAAACTGGAACCCGGAGACACCCTGCTTAGCCTTGGACCACCAGTAACACCAACAG AATAACAGCTGAGTGGAGTTGGG |
| mm4_exon_Oasl2_chr5_114911708 |
TCAAGTTTCTTTTCCTAACAGTCAATTAAGCTGAACCCTGCTTAGTCCCCGACCCCCAACTCTAAACACTCAACTCATAC TGAATGCAGAGTGACACCTTGAGTCACACTGAGAGCTGAGTCACACCTGTGTCT |
| mm4_intron_A330074K22Rik_chr8_120207963 |
AGAAGTCGCTAACCCAGTGCCGGAGGATGAGAGTCCCGGGCCAGGGAAGGCACTGGGCCATGGGTTCGGCGACCAGCAAA GGCAGAGTGGGCAGTCCTCTGGTTTTC |
| mm4_exon_Tbr1_chr2_61811741 |
TTCGTGAGCAACTACGCCAAGGCCCGCTTCCANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNACGGACCGCAGCGTGCC GCACACCAACGGGCTGCTG |
| mm4_intron_Cep170b_chr12_112744319 |
GACCCTCATCCTTGAGCCTGGCCCCCCATCCTCGGGCCTGGACCCCCATCCTCGGGCCAGGACCCCCATCCTCGGGCCTG GACCTCTACCCTCGG |
| mm4_intron_Hhat_chr1_192762961 |
GGCAGCCCTCAAGTCTTCTTTGGGGCCACTCTTCTTCCTTTTCCCATGCTCAGCCCAGGCCCCCCACTCCTTGAACAGCA TGTAAATGG |
| mm4_intergenic_G630030J09Rik/Gramd1a|Scgb1b2_chr7_31180115 |
GGTGGCCAAGAAGGAGCTAGAGTTTGCTAGAATCCTTAGGCTCTTCCATCTTTTGTCCTATAACTAGGGGGTCCCTGGCT GAGCAAGGACCCTAAGGTTATGAGTTTCTGCTGACTTTGCATGCT |
| mm4_intergenic_Polr1a|St3gal5_chr6_72069533 |
GCAAACAAACAGGAGGAGGCCATCTTAGCCATAGGTGTGTGTAGCTCCCAAGCCAGGTCAGCTGTGCTTCAGCAGGACAG GATGGGGGTCCCTGGTGATGCATGGGGGGTGCTGGGGTAGCCTGGGAATATGT |
| mm4_intron_Rps6ka2_chr17_7299452 |
CAGAGTCCCGAGATTGTGCATGGGGCTCCTGGAGCAAGCAAGGCAGTGCCTCAGCATGGTGGGGGTGGTGGGGGTGGTGG AAGGGAGAAAC |
| mm4_intron_Gna11_chr10_81533436 |
CATTGGCCTGAGGGAGGAAGGGACTGTGAACTCCTGTGGGGGGACCCTGGGCAAGCAAGGAGCCTGCAGAGCCAGTAGGG CAGTGAGGGGAGCCAAGGCCTCTAGAAAGC |
| mm4_intergenic_Prune2|Gcnt1_chr19_17243583 |
CACACCCGACTCTGCTTTCTCAGGCTGGACCAACCCTGCTTGCCCCAGGACCCCAGGGGCCAGAAAATTTTGCTCTCTGT GAGCTTAATATCCTCATACATGAGGCACATAATAAATTGTGCGTGTTCTTTCTCCTGCT |
| mm4_intergenic_Me1|A330041J22Rik/Me1_chr9_86688098 |
CTGAGAGAGAAGGTGACGCCTGAGGTGAAGGCTGGGTGGGGGTGCAGGACCCAGCATGGCCATGAACTCAGTGCAGAAAG ATGCAGCTTCTTAAGACAGAAAACAAAAGGAAGCTGTGTCAGCTGCAGGAAACA |
| mm4_intergenic_Gm24880|Gm10172_chr7_71156417 |
GCAGAGCAAGCTGGACAATGATAGAATCGGGTGAGCCCCGTGACTGCCCAGGGACCCCCACTGTACCACCTCTGCTCCGA CTTCACCTG |
| mm4_intergenic_Gm24092|Cenpf_chr1_189541042 |
TGCACACTCATTCCAGGCTTGTGCCCCCACTCCCACCCCCCAGTATCGCCCTACTTGCCCCTGGTCCCCCAAGCAGAGCA AAGAAATCGCTGGAGTCAGACAGATCA |
| mm4_intergenic_Cxcr4|Gm26686_chr1_128709104 |
ACTCCCCTGGTAGGATGACCTGGGGGCCCAGAGGCAAGCATGGAGTCCCACATCCTGCCCCCTTCCTGCATATGCATCAC TTGGTACTAACATGCAGAGAGAGTCTACACCACATCAGCCTCTTGT |
| mm4_intergenic_6530403H02Rik|Rwdd3_chr3_120951249 |
GCCTAAACCCACAATTCTATGCAATCCCCTTGCTGTGCCCCGGGCCCCCATGTACTTCTTTGCCTTCATTAGGCAATTTC TAAGGAAACATCCTGTTCTGAGTCAAGCGC |