CRISPOR

DNA break repair outcome predictions

Guide: TGGGATTAGGAGCTTACTTG GGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

mh- Score	Sequence	Effect
	`AGCCAAGATCCCAGGGAGCCTGGGATTAGGAGCTTACTTGGGGGTTTTCCCCCTCCCCCCCTCTGGAGAGTCCGGGGATG`	(Wild-type sequence)
232	`CCAGGGAGCCTGGG-------------------GTTTTCCCCCTCCCCCCCTCTGGAGAG`	`19 bp deleted → frameshift`
220	`CCAGGGAGCCTGGG--------------------TTTTCCCCCTCCCCCCCTCTGGAGAG`	`20 bp deleted → frameshift`
219	`CCAGGGAGCCTGGGATTAGG------------GGTTTTCCCCCTCCCCCCCTCTGGAGAG`	`12 bp deleted → no frameshift`
208	`CCAGGGAGCCTGGGATTAGG-------------GTTTTCCCCCTCCCCCCCTCTGGAGAG`	`13 bp deleted → frameshift`
198	`CCAGGGAGCCTGGGATTAGG--------------TTTTCCCCCTCCCCCCCTCTGGAGAG`	`14 bp deleted → frameshift`
162	`CCAGGGAGCCTGGG------------------GGTTTTCCCCCTCCCCCCCTCTGGAGAG`	`18 bp deleted → no frameshift`
155	`CCAGGG---------------------------GTTTTCCCCCTCCCCCCCTCTGGAGAG`	`27 bp deleted → no frameshift`
148	`CCAGGG----------------------------TTTTCCCCCTCCCCCCCTCTGGAGAG`	`28 bp deleted → frameshift`
141	`CCAGGGAGCCTGGGATTAGGAGCTTACTT-------TTCCCCCTCCCCCCCTCTGGAGAG`	`7 bp deleted → frameshift`
140	`CCAGGGAGCCTGG---------------------TTTTCCCCCTCCCCCCCTCTGGAGAG`	`21 bp deleted → no frameshift`
134	`CCAGGGAGCCTGGGATTAGGAGCTTACT----------------CCCCCCCTCTGGAGAG`	`16 bp deleted → frameshift`
134	`CCAGGGAGCCTGGGATTAGGAGCTTACTT--------TCCCCCTCCCCCCCTCTGGAGAG`	`8 bp deleted → frameshift`
127	`CCAGGGAGCCTGGGATTAGGAGCTTACTT---------CCCCCTCCCCCCCTCTGGAGAG`	`9 bp deleted → no frameshift`
115	`CCAGGGAGCCTGGGATTAGGAG------------------------------------AG`	`36 bp deleted → no frameshift`
115	`CCAGGGAGCCTGGGATTAGGAGCTT-----------TTCCCCCTCCCCCCCTCTGGAGAG`	`11 bp deleted → frameshift`
110	`CCAGGGAGCCTGGGATTAGGAGCT--------------------CCCCCCCTCTGGAGAG`	`20 bp deleted → frameshift`
109	`CCAGGGAGCCTGGGATTAGGAGCTT------------TCCCCCTCCCCCCCTCTGGAGAG`	`12 bp deleted → no frameshift`
109	`CCAGGG--------------------------GGTTTTCCCCCTCCCCCCCTCTGGAGAG`	`26 bp deleted → frameshift`
104	`CCAGGGAGCCTGGGATTAGGAGCTT-------------CCCCCTCCCCCCCTCTGGAGAG`	`13 bp deleted → frameshift`
96	`CCAGGGAGCCT---------------------------------CCCCCCCTCTGGAGAG`	`33 bp deleted → no frameshift`
94	`CCAGG-----------------------------TTTTCCCCCTCCCCCCCTCTGGAGAG`	`29 bp deleted → frameshift`
90	`CCAGGGAGCCTGGGATTAGGAGCTTACT------------------------CTGGAGAG`	`24 bp deleted → no frameshift`
89	`CCAGGGAGCC------------------------------CCCTCCCCCCCTCTGGAGAG`	`30 bp deleted → no frameshift`
86	`CCAGGGAGCCTGGGATTAGGAGCTTACTTG-------------------------GAGAG`	`25 bp deleted → frameshift`
84	`CCAGGGAGCC-------------------------------CCTCCCCCCCTCTGGAGAG`	`31 bp deleted → frameshift`
81	`CCAGGGAGCCTGGGATTAGGAGCTTACT--------------------------GGAGAG`	`26 bp deleted → frameshift`
81	`CCAGGGAGCCTGG-------------------------------------------AGAG`	`43 bp deleted → frameshift`
80	`CCAGGGAGCC--------------------------------CTCCCCCCCTCTGGAGAG`	`32 bp deleted → frameshift`
77	`CCAGGGAGCCTGGGATT-------------------TTCCCCCTCCCCCCCTCTGGAGAG`	`19 bp deleted → frameshift`
75	`CCAGGGAGCCTGGGATTAGGAG--------------------------------------`	`38 bp deleted → frameshift`
74	`CCAGGGAGCCTGGGATTAGGAGCT----------------------------CTGGAGAG`	`28 bp deleted → frameshift`
73	`CCAGGGAGCCTGGGATT--------------------TCCCCCTCCCCCCCTCTGGAGAG`	`20 bp deleted → frameshift`
70	`CCAGGGAGCCTGGGATT---------------------CCCCCTCCCCCCCTCTGGAGAG`	`21 bp deleted → no frameshift`
66	`CCAGGGAGCCTGGGATTAGGAGCT------------------------------GGAGAG`	`30 bp deleted → no frameshift`
66	`CCAGGGAGCC------------------------------------CCCCCTCTGGAGAG`	`36 bp deleted → no frameshift`
64	`CCAGGGAGCCT-----------------------------------------CTGGAGAG`	`41 bp deleted → frameshift`
62	`CCAGGGAGCC-------------------------------------CCCCTCTGGAGAG`	`37 bp deleted → frameshift`
61	`CCAGGGAGCCTGGGA------------------------------------------GAG`	`42 bp deleted → no frameshift`
60	`CCAGGGAGCC--------------------------------------CCCTCTGGAGAG`	`38 bp deleted → frameshift`
60	`CC--------------------------------------CCCTCCCCCCCTCTGGAGAG`	`38 bp deleted → frameshift`
57	`CCAGGGAG--------------------------------------------------AG`	`50 bp deleted → frameshift`
56	`CCAGGGAGCC---------------------------------------CCTCTGGAGAG`	`39 bp deleted → no frameshift`
56	`CC---------------------------------------CCTCCCCCCCTCTGGAGAG`	`39 bp deleted → no frameshift`
54	`CCAGGGAGCC----------------------------------------CTCTGGAGAG`	`40 bp deleted → frameshift`
54	`CC----------------------------------------CTCCCCCCCTCTGGAGAG`	`40 bp deleted → frameshift`
51	`CC-----------------------------------------TCCCCCCCTCTGGAGAG`	`41 bp deleted → frameshift`
44	`CC--------------------------------------------CCCCCTCTGGAGAG`	`44 bp deleted → frameshift`
42	`CCAGGGAGCCTGGGATTAG---------------------------------------AG`	`39 bp deleted → no frameshift`
42	`CC---------------------------------------------CCCCTCTGGAGAG`	`45 bp deleted → no frameshift`
40	`CC----------------------------------------------CCCTCTGGAGAG`	`46 bp deleted → frameshift`
38	`CCAGGGAGCCTGGGATTAG-----------------------------------------`	`41 bp deleted → frameshift`
38	`CC-----------------------------------------------CCTCTGGAGAG`	`47 bp deleted → frameshift`
37	`CCAGGGAG----------------------------------------------------`	`52 bp deleted → frameshift`
36	`CC------------------------------------------------CTCTGGAGAG`	`48 bp deleted → no frameshift`
34	`CC-------------------------------------------------TCTGGAGAG`	`49 bp deleted → frameshift`
33	`CCAGGGAGCCTGGGA--------------------------------------------G`	`44 bp deleted → frameshift`
31	`CCAGG---------------------------------------------------AGAG`	`51 bp deleted → no frameshift`
20	`CCAG------------------------------------------------------AG`	`54 bp deleted → no frameshift`
18	`CCAG--------------------------------------------------------`	`56 bp deleted → frameshift`

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