CRISPOR

DNA break repair outcome predictions

Guide: CGACCGCGGAGATGGCAGTG GGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

mh- Score	Sequence	Effect
	`CCCCAGCGCCGTGTCGACGGCGACCGCGGAGATGGCAGTGGGGAGCTAGGGTCACCTTGTGATGCGAGCGCTCCTCTGTG`	(Wild-type sequence)
347	`GTGTCGACGGCGACCGCGGAG--------------CTAGGGTCACCTTGTGATGCGAGCG`	`14 bp deleted → frameshift`
282	`GTGTCGACGGCGACCGCGGAGATGG-------GAGCTAGGGTCACCTTGTGATGCGAGCG`	`7 bp deleted → frameshift`
268	`GTGTCGACGGCGACCGCGGAGATGG--------AGCTAGGGTCACCTTGTGATGCGAGCG`	`8 bp deleted → frameshift`
242	`GTGTCGACGGCGACCGCGGAGATGGC----------TAGGGTCACCTTGTGATGCGAGCG`	`10 bp deleted → frameshift`
211	`GTGTCGACGGCGACCGCGGAGATGGCAG-------CTAGGGTCACCTTGTGATGCGAGCG`	`7 bp deleted → frameshift`
208	`GTGTCGACGGCGACCGCGG-------------GAGCTAGGGTCACCTTGTGATGCGAGCG`	`13 bp deleted → frameshift`
188	`GTGTCGACGGCGACCGCGGAGATGG---------------GTCACCTTGTGATGCGAGCG`	`15 bp deleted → no frameshift`
179	`GTGTCGACGGCGACCGCGGAGATGG----------------TCACCTTGTGATGCGAGCG`	`16 bp deleted → frameshift`
175	`GTGTCGACGGCGACCGCGGAGATGGCAGTG---------------------ATGCGAGCG`	`21 bp deleted → no frameshift`
173	`GTGTCGACGGCGACCGCGGAGATGGCAG-----------GGTCACCTTGTGATGCGAGCG`	`11 bp deleted → frameshift`
164	`GTGTCGACGGCGACCGCGGAGA------------GCTAGGGTCACCTTGTGATGCGAGCG`	`12 bp deleted → no frameshift`
156	`GTGTCGACGGCGACCGCGGAGATGGCAGT-------------CACCTTGTGATGCGAGCG`	`13 bp deleted → frameshift`
154	`GTGTCGACGGCGACCGC-------------------TAGGGTCACCTTGTGATGCGAGCG`	`19 bp deleted → frameshift`
140	`GTGTCGACGGCGACCGCGG---------------------GTCACCTTGTGATGCGAGCG`	`21 bp deleted → no frameshift`
133	`GTGTCGACGGCGACCGCGGAGATG------------------------------CGAGCG`	`30 bp deleted → no frameshift`
133	`GTGTCGACGGCGACCGCGG----------------------TCACCTTGTGATGCGAGCG`	`22 bp deleted → frameshift`
133	`GTGTCGACGG----------------------GAGCTAGGGTCACCTTGTGATGCGAGCG`	`22 bp deleted → frameshift`
128	`GTGTCGACGGCGACCGCGGAGATGGCA-----------------CCTTGTGATGCGAGCG`	`17 bp deleted → frameshift`
126	`GTGTCGACGG-----------------------AGCTAGGGTCACCTTGTGATGCGAGCG`	`23 bp deleted → frameshift`
122	`GTGTCGACGGCGACCGCGGAG------------------GGTCACCTTGTGATGCGAGCG`	`18 bp deleted → no frameshift`
116	`GTGTCGACGGCGACCGCGGAGATGGCAGTG-------------------TGATGCGAGCG`	`19 bp deleted → frameshift`
114	`GTGTCGACGGC-------------------------TAGGGTCACCTTGTGATGCGAGCG`	`25 bp deleted → frameshift`
106	`GTGTCGACGGCGACC-------------------------------TTGTGATGCGAGCG`	`31 bp deleted → frameshift`
105	`GTGTCGACGGCGA---------------------GCTAGGGTCACCTTGTGATGCGAGCG`	`21 bp deleted → no frameshift`
94	`GTGTCGACGGCGACCGCGGAGATGGC-----------------------------GAGCG`	`29 bp deleted → frameshift`
90	`GTGTCGACGGCGACCGCGGAGATGGCAGTG------------------------CGAGCG`	`24 bp deleted → no frameshift`
90	`GTGTCGACGGCGACCGCG--------------------------------------AGCG`	`38 bp deleted → frameshift`
89	`GTGTCGACGG------------------------------GTCACCTTGTGATGCGAGCG`	`30 bp deleted → no frameshift`
86	`GTGTCGACGGCGACCGCGGAGATG-------------------------TGATGCGAGCG`	`25 bp deleted → frameshift`
84	`GTGTCGACGG-------------------------------TCACCTTGTGATGCGAGCG`	`31 bp deleted → frameshift`
78	`GTGTCGACGGCGACCGCGGAG-------------------------------------CG`	`37 bp deleted → frameshift`
77	`GTGTCGACGGCGACCGCGGAGATG---------------------------ATGCGAGCG`	`27 bp deleted → no frameshift`
77	`GTGTCGACGGCGA--------------------------------------------GCG`	`44 bp deleted → frameshift`
77	`GTGTCGA---------------------------GCTAGGGTCACCTTGTGATGCGAGCG`	`27 bp deleted → no frameshift`
76	`GTGTCGACGGCGACCGCGGAGATGGC---------------------------------G`	`33 bp deleted → no frameshift`
75	`GTGTC--------------------------------------ACCTTGTGATGCGAGCG`	`38 bp deleted → frameshift`
73	`GTGTCGACGGCGACCGCG------------------------------------------`	`42 bp deleted → no frameshift`
66	`GTGTCGACGGCGACCGCGGAGATGGCAG------------------------------CG`	`30 bp deleted → no frameshift`
60	`GTGTCGACGGCGACCGCGGA--------------------------------TGCGAGCG`	`32 bp deleted → frameshift`
54	`GTGTCGACGGCG------------------------------------------------`	`48 bp deleted → no frameshift`
54	`GTGTCGACGGCGACCG----------------------------------------AGCG`	`40 bp deleted → frameshift`
52	`GTGTCGACGGCGACCGCGGAGA-----------------------------------GCG`	`35 bp deleted → frameshift`
47	`GTGTCGAC-------------------------------------CTTGTGATGCGAGCG`	`37 bp deleted → frameshift`
45	`GTG------------------------------------------------ATGCGAGCG`	`48 bp deleted → no frameshift`
44	`GTGTCGACGGCGACCG--------------------------------------------`	`44 bp deleted → frameshift`
42	`GTGTCGACGGCGA---------------------------------------TGCGAGCG`	`39 bp deleted → no frameshift`
41	`GTGTCGA--------------------------------------------------GCG`	`50 bp deleted → frameshift`
40	`GT----------------------------------------CACCTTGTGATGCGAGCG`	`40 bp deleted → frameshift`
40	`GTGT----------------------------------------------GATGCGAGCG`	`46 bp deleted → frameshift`
38	`GTGTCGACG-----------------------------------------------AGCG`	`47 bp deleted → frameshift`
31	`GTGTCGA---------------------------------------------TGCGAGCG`	`45 bp deleted → no frameshift`
31	`GTGTCGACG---------------------------------------------------`	`51 bp deleted → no frameshift`
26	`GTGTCG------------------------------------------------------`	`54 bp deleted → no frameshift`
23	`GTG---------------------------------------------------CGAGCG`	`51 bp deleted → no frameshift`

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