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Contents:In the table below, Illumina Nextera Handle sequences have been added and highlighted in bold. Primers for the on-target have been added for convenience. The table below is sorted by the CFD off-target score. Sites with very low CFD scores < 0.02 are unlikely to be cleaved, see our study Haeussler et al. 2016, Figure 2.
In the protocol by Matthew Canver, Harvard, two PCRs are run: one PCR to amplify the potential off-target, then a second PCR to extend the handles with Illumina barcodes. Please click here to download the protocol. Alternatively, you can have a look at Fu et al, 2014.
If a primer was not found, the reason is usually that the region around the off-target is too repetitive. To avoid unspecific primers, all repeats are masked for the primer design (not for off-target search). If you think that we should change the parameters here or should use different primer3 settings, please let us know.
| Name | Primer Sequence | Tm | CFD Score |
|---|---|---|---|
| ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52313633_F | TCGTCGGCAGCGTCTTGCAGCAGGAGGATGACAG | 60.0 | 1.00 |
| ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52313633_R | GTCTCGTGGGCTCGGAGATGGCAGTGGGGAGCTA | 59.9 | 1.00 |
| mm4_intron_Adap1_chr5_139279363_F | Primer3: not found at this Tm | N.d. | 0.65 |
| mm4_intron_Adap1_chr5_139279363_R | Primer3: not found at this Tm | N.d. | 0.65 |
| mm4_intergenic_Idh2|Sema4b_chr7_80186630_F | TCGTCGGCAGCGTCAGGAGTGGATTCCAGGGAGA | 59.2 | 0.48 |
| mm4_intergenic_Idh2|Sema4b_chr7_80186630_R | GTCTCGTGGGCTCGGCATACAGTCCACGTAGGCCG | 60.2 | 0.48 |
| mm3_intron_Gfm1_chr3_67442037_F | Primer3: not found at this Tm | N.d. | 0.36 |
| mm3_intron_Gfm1_chr3_67442037_R | Primer3: not found at this Tm | N.d. | 0.36 |
| mm4_intron_Ryr1_chr7_29075547_F | Primer3: not found at this Tm | N.d. | 0.36 |
| mm4_intron_Ryr1_chr7_29075547_R | Primer3: not found at this Tm | N.d. | 0.36 |
| mm4_exon_Angptl4_chr17_33778433_F | Primer3: not found at this Tm | N.d. | 0.35 |
| mm4_exon_Angptl4_chr17_33778433_R | Primer3: not found at this Tm | N.d. | 0.35 |
| mm4_intergenic_Gm4894|Drd2_chr9_49286436_F | Primer3: not found at this Tm | N.d. | 0.30 |
| mm4_intergenic_Gm4894|Drd2_chr9_49286436_R | Primer3: not found at this Tm | N.d. | 0.30 |
| mm4_intron_Col23a1_chr11_51525289_F | Primer3: not found at this Tm | N.d. | 0.29 |
| mm4_intron_Col23a1_chr11_51525289_R | Primer3: not found at this Tm | N.d. | 0.29 |
| mm4_intron_Cdc42bpb_chr12_111329720_F | TCGTCGGCAGCGTCCTCTCAGCATTGCCCGTGAT | 60.4 | 0.29 |
| mm4_intron_Cdc42bpb_chr12_111329720_R | GTCTCGTGGGCTCGGTCCTTACATTTGCCAGTGCCT | 59.9 | 0.29 |
| mm4_exon_Lingo2_chr4_36950433_F | TCGTCGGCAGCGTCGACTCGGGCTCACGTCTC | 59.5 | 0.27 |
| mm4_exon_Lingo2_chr4_36950433_R | GTCTCGTGGGCTCGGGGAGTGGTTGCTCGACGAG | 60.4 | 0.27 |
| mm4_intron_Ralb_chr1_119492328_F | Primer3: not found at this Tm | N.d. | 0.26 |
| mm4_intron_Ralb_chr1_119492328_R | Primer3: not found at this Tm | N.d. | 0.26 |
| mm4_intergenic_Gm12415|Cntln_chr4_84842576_F | Primer3: not found at this Tm | N.d. | 0.25 |
| mm4_intergenic_Gm12415|Cntln_chr4_84842576_R | Primer3: not found at this Tm | N.d. | 0.25 |
| mm4_intron_Ankmy1_chr1_92873266_F | Primer3: not found at this Tm | N.d. | 0.24 |
| mm4_intron_Ankmy1_chr1_92873266_R | Primer3: not found at this Tm | N.d. | 0.24 |
| mm4_intergenic_Actbl2|Gm15285_chr13_111348192_F | TCGTCGGCAGCGTCGGAAATTCCTTCGGGGCTGT | 60.3 | 0.24 |
| mm4_intergenic_Actbl2|Gm15285_chr13_111348192_R | GTCTCGTGGGCTCGGGGCGCTGCTAATTTCTGCTG | 60.2 | 0.24 |
| mm4_intron_Gm281_chr14_13881035_F | TCGTCGGCAGCGTCACTCAGCTCCCCATTCTCAG | 58.7 | 0.24 |
| mm4_intron_Gm281_chr14_13881035_R | GTCTCGTGGGCTCGGAGGTAGACTGAAGGCTCGCT | 60.3 | 0.24 |
| mm4_intergenic_4930517O19Rik|Gm26367_chr14_100323765_F | TCGTCGGCAGCGTCTTAATCCTTGGTTGAATTTTCCCTT | 57.3 | 0.23 |
| mm4_intergenic_4930517O19Rik|Gm26367_chr14_100323765_R | GTCTCGTGGGCTCGGTGACGAATACCCTATTTGTACCCA | 59.2 | 0.23 |
| mm4_intron_Ttc13_chr8_124696996_F | TCGTCGGCAGCGTCAGAAACACCAGCAAGCCCAT | 60.1 | 0.21 |
| mm4_intron_Ttc13_chr8_124696996_R | GTCTCGTGGGCTCGGAAGGGGCTCTTGAATGGTGG | 59.9 | 0.21 |
| mm4_intergenic_Gstm4|Ampd2_chr3_108064009_F | TCGTCGGCAGCGTCACGTCACCTGCAAAACTCCA | 60.1 | 0.20 |
| mm4_intergenic_Gstm4|Ampd2_chr3_108064009_R | GTCTCGTGGGCTCGGATGTGAGAGCCAGGCTTTCC | 60.0 | 0.20 |
| mm4_intergenic_Gm6569/Mroh5|Gm7935_chr15_74048708_F | Primer3: not found at this Tm | N.d. | 0.20 |
| mm4_intergenic_Gm6569/Mroh5|Gm7935_chr15_74048708_R | Primer3: not found at this Tm | N.d. | 0.20 |
| mm4_intron_Rbm19_chr5_120156909_F | TCGTCGGCAGCGTCTTAGCAGTGTCCGGAACTGC | 60.3 | 0.19 |
| mm4_intron_Rbm19_chr5_120156909_R | GTCTCGTGGGCTCGGTCCGACCTCATGTGTCCCAT | 60.6 | 0.19 |
| mm4_intron_Fam65c_chr2_167981234_F | Primer3: not found at this Tm | N.d. | 0.19 |
| mm4_intron_Fam65c_chr2_167981234_R | Primer3: not found at this Tm | N.d. | 0.19 |
| mm4_exon_Ces5a_chr8_93534583_F | TCGTCGGCAGCGTCGGGATAGGCAGTGGCTTCTC | 59.8 | 0.18 |
| mm4_exon_Ces5a_chr8_93534583_R | GTCTCGTGGGCTCGGAAGACTGGAACCCGTGAACG | 60.2 | 0.18 |
| mm4_intergenic_Gm23986|Gm24929_chr8_50853373_F | Primer3: not found at this Tm | N.d. | 0.18 |
| mm4_intergenic_Gm23986|Gm24929_chr8_50853373_R | Primer3: not found at this Tm | N.d. | 0.18 |
| mm4_intron_Ccdc57_chr11_120920292_F | Primer3: not found at this Tm | N.d. | 0.18 |
| mm4_intron_Ccdc57_chr11_120920292_R | Primer3: not found at this Tm | N.d. | 0.18 |
| mm3_intron_Clvs1_chr4_9426993_F | Primer3: not found at this Tm | N.d. | 0.18 |
| mm3_intron_Clvs1_chr4_9426993_R | Primer3: not found at this Tm | N.d. | 0.18 |
| mm4_intron_Gm973_chr1_59601576_F | Primer3: not found at this Tm | N.d. | 0.16 |
| mm4_intron_Gm973_chr1_59601576_R | Primer3: not found at this Tm | N.d. | 0.16 |
| mm4_intron_Gm973_chr1_59601544_F | Primer3: not found at this Tm | N.d. | 0.16 |
| mm4_intron_Gm973_chr1_59601544_R | Primer3: not found at this Tm | N.d. | 0.16 |
| mm4_intron_Gm973_chr1_59601494_F | Primer3: not found at this Tm | N.d. | 0.16 |
| mm4_intron_Gm973_chr1_59601494_R | Primer3: not found at this Tm | N.d. | 0.16 |
| mm4_exon_Hydin_chr8_110597952_F | Primer3: not found at this Tm | N.d. | 0.16 |
| mm4_exon_Hydin_chr8_110597952_R | Primer3: not found at this Tm | N.d. | 0.16 |
| mm4_intron_Exoc2_chr13_30961362_F | TCGTCGGCAGCGTCCAGGGCAGGCAATTCACAAA | 59.3 | 0.16 |
| mm4_intron_Exoc2_chr13_30961362_R | GTCTCGTGGGCTCGGGTCATTGAGAGGCCACAGGT | 59.6 | 0.16 |
| mm4_intron_Afap1_chr5_35984652_F | TCGTCGGCAGCGTCGTTTCACCCAACACAGCTGC | 60.2 | 0.16 |
| mm4_intron_Afap1_chr5_35984652_R | GTCTCGTGGGCTCGGCACAAGGCTCCCAATGCAC | 59.4 | 0.16 |
| mm4_intergenic_Sox2ot|mmu-mir-6378_chr3_34760088_F | Primer3: not found at this Tm | N.d. | 0.16 |
| mm4_intergenic_Sox2ot|mmu-mir-6378_chr3_34760088_R | Primer3: not found at this Tm | N.d. | 0.16 |
| mm3_intergenic_Mir5127|Gm17383_chr18_82234781_F | Primer3: not found at this Tm | N.d. | 0.13 |
| mm3_intergenic_Mir5127|Gm17383_chr18_82234781_R | Primer3: not found at this Tm | N.d. | 0.13 |
| mm3_intergenic_Gm26350|Prlr_chr15_10106199_F | Primer3: not found at this Tm | N.d. | 0.13 |
| mm3_intergenic_Gm26350|Prlr_chr15_10106199_R | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_intergenic_Psg22|Gm24464_chr7_18743452_F | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_intergenic_Psg22|Gm24464_chr7_18743452_R | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_intergenic_mmu-mir-6393|Zdhhc25_chr15_88350075_F | Primer3: not found at this Tm | N.d. | 0.12 |
| mm4_intergenic_mmu-mir-6393|Zdhhc25_chr15_88350075_R | Primer3: not found at this Tm | N.d. | 0.12 |
| mm4_intron_Agpat3_chr10_78302364_F | TCGTCGGCAGCGTCGCTCCTCTTTCACAGTGGCT | 59.9 | 0.11 |
| mm4_intron_Agpat3_chr10_78302364_R | GTCTCGTGGGCTCGGCCAAGACCAACATTCAAGGAAGC | 60.3 | 0.11 |
| mm4_intron_Gsg1l_chr7_126002328_F | TCGTCGGCAGCGTCTGCCCTTTCGTCACACTCAG | 60.2 | 0.10 |
| mm4_intron_Gsg1l_chr7_126002328_R | GTCTCGTGGGCTCGGATGCTCTTTCTCTGCCAGGG | 59.7 | 0.10 |
| mm4_intergenic_Gm5518|Jak2_chr19_29240277_F | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intergenic_Gm5518|Jak2_chr19_29240277_R | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intergenic_OTTMUSG00000011338|CR974586.1_chr4_JH584293_random_160867_F | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intergenic_OTTMUSG00000011338|CR974586.1_chr4_JH584293_random_160867_R | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intergenic_Gm9969|Gm21955_chr4_42702245_F | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intergenic_Gm9969|Gm21955_chr4_42702245_R | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intergenic_Gm2163|Gm13298_chr4_42479516_F | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intergenic_Gm2163|Gm13298_chr4_42479516_R | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intergenic_Gm21598|Gm21586_chr4_42366970_F | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intergenic_Gm21598|Gm21586_chr4_42366970_R | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intergenic_Gm20938|Gm13303_chr4_42014749_F | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intergenic_Gm20938|Gm13303_chr4_42014749_R | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intergenic_CT868723.1|GM21955_chr4_JH584294_random_50054_F | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intergenic_CT868723.1|GM21955_chr4_JH584294_random_50054_R | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intergenic_AC087559.2|FAM205A_chr4_GL456350_random_101156_F | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intergenic_AC087559.2|FAM205A_chr4_GL456350_random_101156_R | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intergenic_1700045I11Rik|Gm17167_chr4_42205098_F | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intergenic_1700045I11Rik|Gm17167_chr4_42205098_R | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intergenic_Nobox|Tpk1_chr6_43317186_F | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intergenic_Nobox|Tpk1_chr6_43317186_R | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intron_Gm15155_chrX_155798379_F | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intron_Gm15155_chrX_155798379_R | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intron_AI314180_chr4_58872883_F | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intron_AI314180_chr4_58872883_R | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intergenic_Gm26256|n-R5s61_chr12_68268055_F | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intergenic_Gm26256|n-R5s61_chr12_68268055_R | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intergenic_5730588L14Rik|Lcn4_chr2_26666179_F | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intergenic_5730588L14Rik|Lcn4_chr2_26666179_R | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intron_Mxi1_chr19_53311725_F | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intron_Mxi1_chr19_53311725_R | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intergenic_Gm26264|Gm22530_chr6_81708417_F | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intergenic_Gm26264|Gm22530_chr6_81708417_R | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_exon_Vpreb1/Top3b_chr16_16870806_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_exon_Vpreb1/Top3b_chr16_16870806_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intron_Tram2_chr1_21032194_F | TCGTCGGCAGCGTCTCCTCTTCCCTCCCTCACAC | 60.2 | 0.07 |
| mm4_intron_Tram2_chr1_21032194_R | GTCTCGTGGGCTCGGCTGAGCAACGTAGTGACCGT | 60.0 | 0.07 |
| mm4_intergenic_Stk10|Fbxw11_chr11_32625881_F | TCGTCGGCAGCGTCCCTGGAAGTCTCGTGACCAG | 59.7 | 0.07 |
| mm4_intergenic_Stk10|Fbxw11_chr11_32625881_R | GTCTCGTGGGCTCGGTGGCTGCCTGCTGATTTACA | 59.9 | 0.07 |
| mm4_intergenic_Sec16b|Brinp2_chr1_157741194_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_Sec16b|Brinp2_chr1_157741194_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intron_Neurl1a_chr19_47193025_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intron_Neurl1a_chr19_47193025_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_Frmpd4|Gm8832_chrX_168190669_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_Frmpd4|Gm8832_chrX_168190669_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_A2m|Mug1_chr6_121837174_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_A2m|Mug1_chr6_121837174_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_Gm23378|Gm22266_chrX_104019327_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_Gm23378|Gm22266_chrX_104019327_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intron_Larp4_chr15_100006292_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intron_Larp4_chr15_100006292_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_Dnaja2|Neto2_chr8_85575260_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_Dnaja2|Neto2_chr8_85575260_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intron_Nav2_chr7_49079173_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intron_Nav2_chr7_49079173_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_Amd2|Hs3st5_chr10_36237480_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_Amd2|Hs3st5_chr10_36237480_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_exon_Foxred2_chr15_77956578_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_exon_Foxred2_chr15_77956578_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intron_Sdk2_chr11_114048175_F | TCGTCGGCAGCGTCACAGAAAGTCAGCAAACACGC | 59.9 | 0.06 |
| mm4_intron_Sdk2_chr11_114048175_R | GTCTCGTGGGCTCGGATTCCAGGCTGTGTAACCCC | 59.6 | 0.06 |
| mm4_intron_Crx_chr7_15869542_F | TCGTCGGCAGCGTCCAGGATAGCTAGGTCTGCTCG | 59.4 | 0.05 |
| mm4_intron_Crx_chr7_15869542_R | GTCTCGTGGGCTCGGGACTGAGCAGCCACTAGGAG | 59.5 | 0.05 |
| mm4_intergenic_A530064D06Rik|Trem1_chr17_48218176_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_A530064D06Rik|Trem1_chr17_48218176_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_exon_Pcdhb9_chr18_37401632_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_exon_Pcdhb9_chr18_37401632_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm3_intergenic_Gm4262|Litaf_chr16_11026825_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm3_intergenic_Gm4262|Litaf_chr16_11026825_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intron_Tm4sf20_chr1_82762195_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intron_Tm4sf20_chr1_82762195_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_Cacna1h|Tekt4_chr17_25449501_F | TCGTCGGCAGCGTCCCTGCTCTGTGGAGAAAGCT | 59.6 | 0.05 |
| mm4_intergenic_Cacna1h|Tekt4_chr17_25449501_R | GTCTCGTGGGCTCGGGCAAGGGGAAGTGTCTAGGG | 59.7 | 0.05 |
| mm4_exon_Hira_chr16_18877250_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_exon_Hira_chr16_18877250_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm3_exon_Hk3_chr13_55012248_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm3_exon_Hk3_chr13_55012248_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intron_Als2cr11_chr1_59091131_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intron_Als2cr11_chr1_59091131_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_Nfam1|Serhl_chr15_83076156_F | TCGTCGGCAGCGTCACGTTTCTGAGCTGGCATCA | 59.9 | 0.04 |
| mm4_intergenic_Nfam1|Serhl_chr15_83076156_R | GTCTCGTGGGCTCGGATGTCTAACGGGCAGAGGGA | 60.3 | 0.04 |
| mm4_intergenic_Gm23389|Gm23181_chr1_94680120_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Gm23389|Gm23181_chr1_94680120_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Gm5432|Lpin1_chr12_16491462_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Gm5432|Lpin1_chr12_16491462_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Lsm6|Gm8054_chr8_78849085_F | TCGTCGGCAGCGTCACTCTGTGCCAGTGCTGTTT | 60.1 | 0.04 |
| mm4_intergenic_Lsm6|Gm8054_chr8_78849085_R | GTCTCGTGGGCTCGGACCAAAGACAGCTTAGATTTCTGTG | 59.5 | 0.04 |
| mm4_exon_Ak4_chr4_101419309_F | TCGTCGGCAGCGTCCCCGGCTTACGTAAGAAGCA | 60.1 | 0.04 |
| mm4_exon_Ak4_chr4_101419309_R | GTCTCGTGGGCTCGGGCCAGACTTAGCCCGGATC | 59.9 | 0.04 |
| mm4_intergenic_Tmsb10|Dnah6_chr6_72982615_F | TCGTCGGCAGCGTCTCTGCACAGTCCAACAGGGA | 61.0 | 0.04 |
| mm4_intergenic_Tmsb10|Dnah6_chr6_72982615_R | GTCTCGTGGGCTCGGCCTCCAACACTGGTACCCAC | 59.9 | 0.04 |
| mm4_intergenic_Gm23218|Fyb_chr15_6569891_F | TCGTCGGCAGCGTCGGGACCTGTCTACCAGTTGC | 60.0 | 0.04 |
| mm4_intergenic_Gm23218|Fyb_chr15_6569891_R | GTCTCGTGGGCTCGGTAGGGTAGTGCCTCTGGACC | 60.0 | 0.04 |
| mm4_intron_Dhx35_chr2_158795206_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intron_Dhx35_chr2_158795206_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Capn10|Gpr35_chr1_92958071_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Capn10|Gpr35_chr1_92958071_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Papss1|Gm26103_chr3_131703747_F | TCGTCGGCAGCGTCGGAGCATGCAGAACTTGTGC | 60.1 | 0.04 |
| mm4_intergenic_Papss1|Gm26103_chr3_131703747_R | GTCTCGTGGGCTCGGCCTCTGTCAAGTCTGCAGGG | 60.0 | 0.04 |
| mm4_intergenic_Max|Gm22696_chr12_76984591_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Max|Gm22696_chr12_76984591_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intron_Dpp10_chr1_123632813_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intron_Dpp10_chr1_123632813_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm3_intergenic_Stxbp3b|Scgb2a2_chr19_9701575_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm3_intergenic_Stxbp3b|Scgb2a2_chr19_9701575_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm3_intergenic_Gm26119|Fzd8_chr18_8822308_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm3_intergenic_Gm26119|Fzd8_chr18_8822308_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Prickle1|Adamts20_chr15_93930796_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Prickle1|Adamts20_chr15_93930796_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Las1l|Msn_chrX_95968259_F | TCGTCGGCAGCGTCGCTTCTAACCACCATGGCCT | 60.0 | 0.03 |
| mm4_intergenic_Las1l|Msn_chrX_95968259_R | GTCTCGTGGGCTCGGGGCGGAAGGAAAGAGAGGTC | 60.1 | 0.03 |
| mm4_intergenic_Exog|Scn5a_chr9_119476560_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Exog|Scn5a_chr9_119476560_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intron_Pgf_chr12_85169295_F | TCGTCGGCAGCGTCGGATTCCCTGTTCTCATCGCA | 60.1 | 0.03 |
| mm4_intron_Pgf_chr12_85169295_R | GTCTCGTGGGCTCGGCACAGACTGAGGAACCCCAC | 59.9 | 0.03 |
| mm4_intergenic_Gm26820|Tacr3_chr3_134711108_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Gm26820|Tacr3_chr3_134711108_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Actrt2|Gm13113_chr4_154695069_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Actrt2|Gm13113_chr4_154695069_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Klhl38|Gm15943/Klhl38_chr15_58319277_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Klhl38|Gm15943/Klhl38_chr15_58319277_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intron_Rreb1_chr13_37918942_F | TCGTCGGCAGCGTCCCGGGTGGTACATTGTGACA | 59.9 | 0.03 |
| mm4_intron_Rreb1_chr13_37918942_R | GTCTCGTGGGCTCGGCGGACTCTGGTTCTAAACTGGT | 59.7 | 0.03 |
| mm4_intergenic_Emx2|Gm17203_chr19_59699192_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Emx2|Gm17203_chr19_59699192_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Pi4ka|Snap29/Pi4ka_chr16_17405953_F | TCGTCGGCAGCGTCACCTGACCACCTGACTCCTC | 60.5 | 0.03 |
| mm4_intergenic_Pi4ka|Snap29/Pi4ka_chr16_17405953_R | GTCTCGTGGGCTCGGCACTACTTCCGCCAGCACTG | 61.0 | 0.03 |
| mm4_intergenic_Gm26002|Itgb1_chr8_128681165_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Gm26002|Itgb1_chr8_128681165_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intron_Ddr2_chr1_170088027_F | TCGTCGGCAGCGTCTCCACAGTTCCACCTGCTTC | 59.8 | 0.03 |
| mm4_intron_Ddr2_chr1_170088027_R | GTCTCGTGGGCTCGGTGCATCTTCACCTGGCATGT | 59.9 | 0.03 |
| mm4_intron_Sh3rf1_chr8_61264402_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intron_Sh3rf1_chr8_61264402_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Gm22227|Zfp703_chr8_26784715_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Gm22227|Zfp703_chr8_26784715_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Scarf1|Slc43a2_chr11_75529704_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Scarf1|Slc43a2_chr11_75529704_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Gm10610|Tmc3_chr7_83564303_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Gm10610|Tmc3_chr7_83564303_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Tpd52|Zbtb10_chr3_9098780_F | TCGTCGGCAGCGTCCTGCTCACAAAATGCGTGCT | 60.0 | 0.02 |
| mm4_intergenic_Tpd52|Zbtb10_chr3_9098780_R | GTCTCGTGGGCTCGGGCAACAGCGACAACAAAAGC | 59.4 | 0.02 |
| mm4_intergenic_Snx29|Cpped1_chr16_11801929_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Snx29|Cpped1_chr16_11801929_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intron_Myo9a_chr9_59791307_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intron_Myo9a_chr9_59791307_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Gm12943|Dlgap3_chr4_127168463_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Gm12943|Dlgap3_chr4_127168463_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intron_Slit3_chr11_35690585_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intron_Slit3_chr11_35690585_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intron_Mro_chr18_73873506_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intron_Mro_chr18_73873506_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intron_Man2a1_chr17_64611803_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intron_Man2a1_chr17_64611803_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Trpm7|Sppl2a_chr2_126881574_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Trpm7|Sppl2a_chr2_126881574_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Ptprg|3830406C13Rik_chr14_12283376_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Ptprg|3830406C13Rik_chr14_12283376_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Mir598|Xkr6_chr14_63777419_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Mir598|Xkr6_chr14_63777419_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Gm23180|Gm22977_chr16_54965979_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Gm23180|Gm22977_chr16_54965979_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Zfp619|Gm26526/Gm2058_chr7_39552742_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Zfp619|Gm26526/Gm2058_chr7_39552742_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Gm23385|Gm7803_chrX_50121233_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Gm23385|Gm7803_chrX_50121233_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intron_Mgmt_chr7_136918271_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intron_Mgmt_chr7_136918271_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_exon_Lef1_chr3_131223382_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_exon_Lef1_chr3_131223382_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intron_Adamts19_chr18_59010332_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intron_Adamts19_chr18_59010332_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Ago1|Gm12929_chr4_126468788_F | TCGTCGGCAGCGTCACAAAGGCCAATGGAGAGGG | 59.9 | 0.02 |
| mm4_intergenic_Ago1|Gm12929_chr4_126468788_R | GTCTCGTGGGCTCGGAGCCCCTCCCCTCTTACG | 60.0 | 0.02 |
| mm4_intergenic_Msr1|Fgf20_chr8_39814339_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Msr1|Fgf20_chr8_39814339_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_exon_Ripk1/Serpinb6a_chr13_34002476_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_exon_Ripk1/Serpinb6a_chr13_34002476_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_Myh14_chr7_44670705_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_Myh14_chr7_44670705_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Abca4|Gm4609_chr3_122189972_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Abca4|Gm4609_chr3_122189972_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Pacrg|A230009B12Rik_chr17_10454762_F | TCGTCGGCAGCGTCTTGGTCATCACAGCCTGCAT | 59.9 | 0.01 |
| mm4_intergenic_Pacrg|A230009B12Rik_chr17_10454762_R | GTCTCGTGGGCTCGGCCTAAACCGCAAGTCCTGGT | 59.9 | 0.01 |
| mm4_intergenic_A530013C23Rik|Gm14321_chr2_167769919_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_A530013C23Rik|Gm14321_chr2_167769919_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_Prkd1_chr12_50568199_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_Prkd1_chr12_50568199_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Nps|4930544L04Rik_chr7_135296933_F | TCGTCGGCAGCGTCGGCAAGCCTGAGTCAAGTCT | 59.9 | 0.01 |
| mm4_intergenic_Nps|4930544L04Rik_chr7_135296933_R | GTCTCGTGGGCTCGGGCAGAACCCTGGGATGGAAA | 59.9 | 0.01 |
| mm4_intron_2900026A02Rik_chr5_113104448_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_2900026A02Rik_chr5_113104448_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Uimc1|Zfp346_chr13_55105172_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Uimc1|Zfp346_chr13_55105172_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Stk35|Tgm3_chr2_129921276_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Stk35|Tgm3_chr2_129921276_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm3_intergenic_Gm17177|Speer6-ps1_chr13_3185164_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm3_intergenic_Gm17177|Speer6-ps1_chr13_3185164_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_Dgkd_chr1_87931291_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Dgkd_chr1_87931291_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Cdh22_chr2_165180868_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Cdh22_chr2_165180868_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm3_intergenic_Gm24120|Nr2f2_chr7_69985073_F | TCGTCGGCAGCGTCATTCCTCTTCCCCATCTCCA | 57.4 | 0.00 |
| mm3_intergenic_Gm24120|Nr2f2_chr7_69985073_R | GTCTCGTGGGCTCGGGGGGAAGCAAAGGTGAGCAA | 60.8 | 0.00 |
| mm4_intron_Srrm1_chr4_135332619_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Srrm1_chr4_135332619_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Ctbp2_chr7_133037472_F | TCGTCGGCAGCGTCTAGAGGGCCAACAAAACCCC | 59.8 | 0.00 |
| mm4_intron_Ctbp2_chr7_133037472_R | GTCTCGTGGGCTCGGGACCCATTGACCCTCTGTGG | 60.0 | 0.00 |
| mm4_intergenic_Upp2|Gm13558_chr2_58680352_F | TCGTCGGCAGCGTCCTGGTGGCCATTCTCTTGGT | 59.9 | 0.00 |
| mm4_intergenic_Upp2|Gm13558_chr2_58680352_R | GTCTCGTGGGCTCGGAGGCTACTGCAAACCCAGAC | 59.9 | 0.00 |
| mm4_intergenic_S100a4|S100a5_chr3_90606364_F | TCGTCGGCAGCGTCGTTAGCCAGCCCACAGTTCT | 59.9 | 0.00 |
| mm4_intergenic_S100a4|S100a5_chr3_90606364_R | GTCTCGTGGGCTCGGGCAGGCCTGTTGTCTTCTCT | 59.9 | 0.00 |
| mm4_intron_Plk3_chr4_117130445_F | TCGTCGGCAGCGTCCTGGGTTCTGTTCGGCTGTT | 60.5 | 0.00 |
| mm4_intron_Plk3_chr4_117130445_R | GTCTCGTGGGCTCGGAGGTAAGGCTGGACTCTGGT | 59.8 | 0.00 |
These only list off-targets that have primers in the table above. Primers underlined, off-targets in bold.
| ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52313633 |
TTGCAGCAGGAGGATGACAGCTTAGAAAGAAGAGGGCAATGGGGCTTCCTCCCAGAGGCGGTGCGGCACAGAGGAGCGCT CGCATCACAAGGTGACCCTAGCTCCCCACTGCCATCT |
| mm4_intergenic_Idh2|Sema4b_chr7_80186630 |
AGGAGTGGATTCCAGGGAGACCACCCACCAGCGAGGCTGAACATGCCAAGGTCAGAGAGGGTGCAGCACAGTGGACTTCG GCCTACGTGGACTGTATG |
| mm4_intron_Cdc42bpb_chr12_111329720 |
CTCTCAGCATTGCCCGTGATGGCACCACACATGGTAGACTGTGGAAAATGCTATGGAGAGATCTAAAAGGAGCTCAGGGG CCGTGCAGCACAGAGGGAGGCACTGGCAAATGTAAGGA |
| mm4_exon_Lingo2_chr4_36950433 |
GACTCGGGCTCACGTCTCCCGCCCAGGCTGGGTTGGACCCCCTATGCCGCGCCGCCTCAGCCACCAGGCTCGCTCGTCGA GCAACCACTCC |
| mm4_intergenic_Actbl2|Gm15285_chr13_111348192 |
GGAAATTCCTTCGGGGCTGTGGTCGCCCGACATTTGCCATCACAAAAGATAGCCATACCAGAGGAGGTACAGCAAAGAGG TAGAACCCCAAGAAAAATCTGACGCAGCAGAAATTAGCAGCGCC |
| mm4_intron_Gm281_chr14_13881035 |
ACTCAGCTCCCCATTCTCAGATTCCAAGGCATGAGGACAGATTATAGGCCCAGAGGATATGCAGCACAGGGGAGCGAGCC TTCAGTCTACCT |
| mm4_intergenic_4930517O19Rik|Gm26367_chr14_100323765 |
TTAATCCTTGGTTGAATTTTCCCTTCCACTGTGCCTCATTGCTTCTGGCATTTGTATCAAAAGGGTTTAACAAACCTTTC CTTATTATTATTAAAATATCAATGTCATTTAAAGTGGGTACAAATAGGGTATTCGTCA |
| mm4_intron_Ttc13_chr8_124696996 | AGAAACACCAGCAAGCCCATCCAGAGCTGGTGAGACACAGTGGCCAGCCCGACCACCATTCAAGAGCCCCTT |
| mm4_intergenic_Gstm4|Ampd2_chr3_108064009 |
ACGTCACCTGCAAAACTCCAGAGGTTTCTAGAACACTAGAGGTGGTACGCCACAGTGGCTCAGATTGGAATCTGGAGTCA AATGAGAATTGGAAAGCCTGGCTCTCACAT |
| mm4_intron_Rbm19_chr5_120156909 |
TTAGCAGTGTCCGGAACTGCCAGTGTTGTCATGATTCCGAAGGTACCTACTCTGTGTAGACACCACTGTGCCGCACATCC TCCAGAGCATGGGACACATGAGGTCGGA |
| mm4_exon_Ces5a_chr8_93534583 |
GGGATAGGCAGTGGCTTCTCGCAAATTATCCCAGGGGATCGGAGGCTGTGGCTTGGAAAACCGCAGAGGTCCAAGAGGGG GTGCAGCAAAGGGGATCCCAAGAAACACGTTCACGGGTTCCAGTCTT |
| mm4_intron_Exoc2_chr13_30961362 |
CAGGGCAGGCAATTCACAAATGAGGAGTTGAAAGCAATAAATGTGGAAAAACAAATTTGGCCTCAGAGATGCACCAGAGC TGGGGTGGCACAGTGGGCTCTATCTGAGCTCTGAAAACCTGTGGCCTCTCAATGAC |
| mm4_intron_Afap1_chr5_35984652 |
GTTTCACCCAACACAGCTGCAGCCGCCATCACCCGGAAGCAGTGCCGCACAGTGGCTGCCTGCTGTGCCCTCGCTTGGCT CCTGGCTATGCCTGTGCAGTGAGCAGGTGTGGGGCAGCGTGCATTGGGAGCCTTGTG |
| mm4_intron_Agpat3_chr10_78302364 |
GCTCCTCTTTCACAGTGGCTTTGGAAAGCTTTAACCCACTGTGCCTCTCAGCCCCTGGGCTTCCTTGAATGTTGGTCTTG G |
| mm4_intron_Gsg1l_chr7_126002328 |
TGCCCTTTCGTCACACTCAGGACCATATTAACCAATGGGAAACAAAACCCCGCTCCGGCCAAAGGCACAGAGTCAGGGGT CTCCCCAGGTTCTGCACCGCCTCTGGCTTCCCTGGCAGAGAAAGAGCAT |
| mm4_intron_Tram2_chr1_21032194 |
TCCTCTTCCCTCCCTCACACCTGCAGAGGCAGAGCGGCCCAGAGGCCCGTGGATCTCAGATAGCAAGATTCCAAACTGAG AGGAAAGAAATAACTGATGATACGGTCACTACGTTGCTCAG |
| mm4_intergenic_Stk10|Fbxw11_chr11_32625881 |
CCTGGAAGTCTCGTGACCAGCAGAGGGCAGGCCATGGCCATGGTGCTGCACAGCCTCTGGACTCAGGCTGTTGGTGTGGC TGCAGCTTCCACTGATGAGTACTTACTGTCTGGGGGATGTAAATCAGCAGGCAGCCA |
| mm4_intron_Sdk2_chr11_114048175 |
ACAGAAAGTCAGCAAACACGCTGGACCCGCCCCTGTGCCCCACTGCGTCTGTGTGTCTGTGTAGGCAGATCCCCCAGCAA CGCAGCCACAGCCTCCCTGACCTGCAGGCTGACCCTCGGGGTTACACAGCCTGGAAT |
| mm4_intron_Crx_chr7_15869542 |
CAGGATAGCTAGGTCTGCTCGCCAGAGGCCATGAGGTACAGAGGAATACCTGTCCCAAAGACCTTTCTTCACGTCCATCA GCCTAAACAAGGAGCTTTGATTGATTCTCTGGCCTCCTAGTGGCTGCTCAGTC |
| mm4_intergenic_Cacna1h|Tekt4_chr17_25449501 |
CCTGCTCTGTGGAGAAAGCTGTTCATCAGGTGTCCAGGACTCCCCTGGGCCTCACCACCTCTGCTGGGTCAGCTCCCTTT GAGTTAGAAAGCCACTATGGTCCTGGAAGTGCCTACCCCTAGACACTTCCCCTTGC |
| mm4_intergenic_Nfam1|Serhl_chr15_83076156 |
ACGTTTCTGAGCTGGCATCACGCCGCGTTCGATTGCGCAATGTGTGTTTACAGGTGAGATTGACTCCCACGTCCTCTGTG CCCCTCCGCCTGTGCCGCAGGACTTCCTGCCAGTCCCTCTGCCCGTTAGACAT |
| mm4_intergenic_Lsm6|Gm8054_chr8_78849085 |
ACTCTGTGCCAGTGCTGTTTATTTAAAATAAAACAAATCTTTTAAAAAAGAAACCCTGTACTAGCACAGAAGGCCACTGG GCCACACAGCCTCAGGAACCATTGACAGATCACAGAAATCTAAGCTGTCTTTGGT |
| mm4_exon_Ak4_chr4_101419309 |
CCCGGCTTACGTAAGAAGCAGCGAGTCCCGCGCGGCCAGCCCGGCAGAGGCGGCGCCGCCCAGCGGGATCCGGGCTAAGT CTGGC |
| mm4_intergenic_Tmsb10|Dnah6_chr6_72982615 |
TCTGCACAGTCCAACAGGGAGGTATGAAAGTCTTTGGCACAAGCAATCTGGGCTTTAACAATGGTCAGTTAATGTGGGTA GGACAGGCCACTAGGCTGCACAGCCTCTGGGTTGAAGGTTGTGGGTACCAGTGTTGGAGG |
| mm4_intergenic_Gm23218|Fyb_chr15_6569891 |
GGGACCTGTCTACCAGTTGCGTTTGCAGGCCTCTGTGCCCTGCAGCCTCTGGTCTTGCACGTGCTTCTGGTCCAGAGGCA CTACCCTA |
| mm4_intergenic_Papss1|Gm26103_chr3_131703747 |
GGAGCATGCAGAACTTGTGCTATGATTGATTCTTTTCTCCTCTGTCCCTCACCGCCTCTCTGCCATTCCATTGCTCCTGC CCTGCAGACTTGACAGAGG |
| mm4_intergenic_Las1l|Msn_chrX_95968259 |
GCTTCTAACCACCATGGCCTCTCTGGCTCTTTGCACTCCAGCCTCTATGCCCCTCGGCCTCTGGACCTACATCTCTGTAG CCTAGGCATCTCTGTCCCTCGCTCTATGCCCCTAGACCTCTCTTTCCTTCCGCC |
| mm4_intron_Pgf_chr12_85169295 |
GGATTCCCTGTTCTCATCGCAAGACCTCATCCCCAGTGCCCCACCCCCTCAGGCTCAGCCTTTGTGCCAGTCCTGGGCCC CAGCATACTCACGGGTGGGGTTCCTCAGTCTGTG |
| mm4_intron_Rreb1_chr13_37918942 |
CCGGGTGGTACATTGTGACAGTAGCTGATGGGAGAGACTGCTTGTTAACCCAGAACTAAAATAGACACCAACTCCATTTT CAAATCCCAGAGGCGCAGAGCCACAGTGGGATGCTACCAGTTTAGAACCAGAGTCCG |
| mm4_intergenic_Pi4ka|Snap29/Pi4ka_chr16_17405953 |
ACCTGACCACCTGACTCCTCCCCTCTCCCCTCCCCCGCACTGGGTGGGGCCTGGTCCCCCTCTGCCGCTCCGCCGCCGGG TTGGCTCTGGAGGCGGGGCTCTAGCGCAGTGCTGGCGGAAGTAGTG |
| mm4_intron_Ddr2_chr1_170088027 |
TCCACAGTTCCACCTGCTTCGCTCAGTCATTCAACACTCAACAGTATTCCAGAGGCAGTGTGGGACACAGGCAAGTCTAA GGCAATCCCCACTGAGCAGTCTTCACATGCCAGGTGAAGATGCA |
| mm4_intergenic_Tpd52|Zbtb10_chr3_9098780 |
CTGCTCACAAAATGCGTGCTCCGCCCCAGGCAGGACGGTCCGTCCTCCGAGCTGCTCCGCCTCTGGGGAACCTGGTTGGA AAAGACCAGTGAATAGACTTAAGGCTTTTGTTGTCGCTGTTGC |
| mm4_intergenic_Ago1|Gm12929_chr4_126468788 |
ACAAAGGCCAATGGAGAGGGGCGGGGGCGCGGGGACCGTGCAGCGGCCTCTGGCGGCCACTCGTAGCCGTGCAGCCTGGC GGGAGGGCTCCAGTGGCGGAGCCGCGCAGTGGCGTAAGAGGGGAGGGGCT |
| mm4_intergenic_Pacrg|A230009B12Rik_chr17_10454762 |
TTGGTCATCACAGCCTGCATCACATCCTTTTAGGACCGCTGTGATCACTAGCCAATTCATCCACTGTGGGGCAGCGCCTA TGGCTTTCCTTCTGATCACCAGGACTTGCGGTTTAGG |
| mm4_intergenic_Nps|4930544L04Rik_chr7_135296933 |
GGCAAGCCTGAGTCAAGTCTCCCATCAATATTTGACTTGCCAGTGTTTCTATGGCCTCAGCCCCGCTGCCACACCTCCTC TGGGCAGACCTGCCACAAGAGAGTCTTCTAGGTGCATTTGTTTCCATCCCAGGGTTCTGC |
| mm3_intergenic_Gm24120|Nr2f2_chr7_69985073 | ATTCCTCTTCCCCATCTCCATCTCTGTGCCCCACCACCTCTGCTTGCTCACCTTTGCTTCCCC |
| mm4_intron_Ctbp2_chr7_133037472 |
TAGAGGGCCAACAAAACCCCTTTTAATTGTCCTCTGACCAGCTCCGCCTCTGGGGTCCCCTGTCTGGGATCCTGACAGGC AAATAGTCTTTGTTCACCCACAGAGGGTCAATGGGTC |
| mm4_intergenic_Upp2|Gm13558_chr2_58680352 |
CTGGTGGCCATTCTCTTGGTAGAGGATGACTTATTGTTTAGAGGGTTAGGCCAGAGGCGGTGGGTCACCATGGTCTGTCA TGGTGTCCCCTTGTTGAAGCAGCAAATGTCTGGGTTTGCAGTAGCCT |
| mm4_intergenic_S100a4|S100a5_chr3_90606364 |
GTTAGCCAGCCCACAGTTCTCCCTCCTCTCTCCAGCACTGCCTCTGGGCTCAAACTGAAGTTGGGATGGGATTAAAGGTC ACATCTGCTGCTGGTTGGGGTCTGGAGAGAAGACAACAGGCCTGC |
| mm4_intron_Plk3_chr4_117130445 |
CTGGGTTCTGTTCGGCTGTTAAGAGGAGAGGAGGGAAATGAAGACTCCCCCAACCCCCCTCCCCCCCACCGCCTCTGGAC TTGCAAACTATTCATATGCACCAGAGTCCAGCCTTACCT |