CRISPOR

DNA break repair outcome predictions

Guide: AGGAGCTAAGGCGACAAGGC AGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

mh- Score	Sequence	Effect
	`CTGGGATCTTGGCTGAGAAGAGGAGCTAAGGCGACAAGGCAGGCGCCGGGTAGCCCCAGCGCCGTGTCGACGGCGACCGC`	(Wild-type sequence)
573	`GGCTGAGAAGAGGAGCTAAGGCGACAAGGC----GCCGGGTAGCCCCAGCGCCGTGTCGA`	`4 bp deleted → frameshift`
494	`GGCTGAGAAGAGGAGCTAAGGCG------------CCGGGTAGCCCCAGCGCCGTGTCGA`	`12 bp deleted → no frameshift`
296	`GGCTGAGAAGAGGAGCTAAGGCGACAAGGC------CGGGTAGCCCCAGCGCCGTGTCGA`	`6 bp deleted → no frameshift`
242	`GGCTGAGAAGAGGAGCTAAGGCGACAAGG----------GTAGCCCCAGCGCCGTGTCGA`	`10 bp deleted → frameshift`
230	`GGCTGAGAAGAGGAGCTAAGGCGACAAGG-----------TAGCCCCAGCGCCGTGTCGA`	`11 bp deleted → frameshift`
198	`GGCTGAGAAGAGGAGCTAAGGCGACAAGGC--------------CCCAGCGCCGTGTCGA`	`14 bp deleted → frameshift`
198	`GGCTGAGAAGAGGAGCTAAGGC--------------CGGGTAGCCCCAGCGCCGTGTCGA`	`14 bp deleted → frameshift`
188	`GGCTGAGAAGAGGAGCTAAGGCG---------------GGTAGCCCCAGCGCCGTGTCGA`	`15 bp deleted → no frameshift`
184	`GGCTGAGAAGAGG--------------------CGCCGGGTAGCCCCAGCGCCGTGTCGA`	`20 bp deleted → frameshift`
162	`GGCTGAGAAGAGGAGCTAAGG------------------GTAGCCCCAGCGCCGTGTCGA`	`18 bp deleted → no frameshift`
162	`GGCTGAGAAGAGGAGC------------------GCCGGGTAGCCCCAGCGCCGTGTCGA`	`18 bp deleted → no frameshift`
154	`GGCTGAGAAGAGGAGCTAAGG-------------------TAGCCCCAGCGCCGTGTCGA`	`19 bp deleted → frameshift`
148	`GGCTGAGAAGAGGAGCTAAGGCG----------------------------CCGTGTCGA`	`28 bp deleted → frameshift`
147	`GGCTGAGAAGAGGAGCTAAGGCGACAAGGC--------------------GCCGTGTCGA`	`20 bp deleted → frameshift`
147	`GGCTGAGAAGAGGAGC--------------------CGGGTAGCCCCAGCGCCGTGTCGA`	`20 bp deleted → frameshift`
141	`GGCTGAGAAGAGGAGCTAAGGCGACAAG---------------CCCCAGCGCCGTGTCGA`	`15 bp deleted → no frameshift`
133	`GGCTGAGAAGAGGAGCTAAGGCGACAAGGC----------------------CGTGTCGA`	`22 bp deleted → frameshift`
133	`GGCTGAGAAGAGGAGCTAAGGC----------------------CCCAGCGCCGTGTCGA`	`22 bp deleted → frameshift`
128	`GGCTGAGAAGAGGAG-----------------GCGCCGGGTAGCCCCAGCGCCGTGTCGA`	`17 bp deleted → frameshift`
127	`GGC-------------------------------GCCGGGTAGCCCCAGCGCCGTGTCGA`	`31 bp deleted → frameshift`
123	`GGCTGAGAAGAGGAGC----------------------------CCCAGCGCCGTGTCGA`	`28 bp deleted → frameshift`
109	`GGCTGAGAAGAGG--------------------------GTAGCCCCAGCGCCGTGTCGA`	`26 bp deleted → frameshift`
105	`GGCTGAGAAGAGGAGCTAAGGCGACAAG---------------------CGCCGTGTCGA`	`21 bp deleted → no frameshift`
103	`GGCTGAGAAGAGG---------------------------TAGCCCCAGCGCCGTGTCGA`	`27 bp deleted → no frameshift`
99	`GGCTGAGAAGAGGAGCTAAGGCGACA----------------------GCGCCGTGTCGA`	`22 bp deleted → frameshift`
99	`GGCTGAGAAG----------------------GCGCCGGGTAGCCCCAGCGCCGTGTCGA`	`22 bp deleted → frameshift`
95	`GGCTGAGAAGAGGAGCTAAG-----------------------CCCCAGCGCCGTGTCGA`	`23 bp deleted → frameshift`
91	`GGCTGAGAAGAGGAGC----------------------------------GCCGTGTCGA`	`34 bp deleted → frameshift`
89	`GGCTGAGAAGAGGAGCTAAGGC------------------------------CGTGTCGA`	`30 bp deleted → no frameshift`
86	`GGCTGAG-------------------------GCGCCGGGTAGCCCCAGCGCCGTGTCGA`	`25 bp deleted → frameshift`
84	`GGCTGAGAAGAGGAGCTAAGGCG-------------------------------TGTCGA`	`31 bp deleted → frameshift`
82	`GGCTGAGAAGAGGAGCTAAGGCGA------------------------------------`	`36 bp deleted → no frameshift`
76	`GGC---------------------------------CGGGTAGCCCCAGCGCCGTGTCGA`	`33 bp deleted → no frameshift`
70	`GGCTGAGAAGAGGAGCTAAG-----------------------------CGCCGTGTCGA`	`29 bp deleted → frameshift`
66	`GGCTGAGAAGAGGAGC------------------------------------CGTGTCGA`	`36 bp deleted → no frameshift`
63	`GGCTGAGAAGAG-------------------------------CCCCAGCGCCGTGTCGA`	`31 bp deleted → frameshift`
62	`GG-------------------------------------GTAGCCCCAGCGCCGTGTCGA`	`37 bp deleted → frameshift`
60	`GGCTGAGAAGAGGAGCTA------------------------GCCCCAGCGCCGTGTCGA`	`24 bp deleted → no frameshift`
60	`GG--------------------------------------TAGCCCCAGCGCCGTGTCGA`	`38 bp deleted → frameshift`
57	`GGCTGAGAAG---------------------------------CCCCAGCGCCGTGTCGA`	`33 bp deleted → no frameshift`
51	`GGC-----------------------------------------CCCAGCGCCGTGTCGA`	`41 bp deleted → frameshift`
49	`GGCTGAG------------------------------------CCCCAGCGCCGTGTCGA`	`36 bp deleted → no frameshift`
47	`GGCTGAGAAGAG-------------------------------------CGCCGTGTCGA`	`37 bp deleted → frameshift`
42	`GGCTGAGAAG---------------------------------------CGCCGTGTCGA`	`39 bp deleted → no frameshift`
38	`GGC-----------------------------------------------GCCGTGTCGA`	`47 bp deleted → frameshift`
36	`GGCTGAG------------------------------------------CGCCGTGTCGA`	`42 bp deleted → no frameshift`
34	`GGC-------------------------------------------------CGTGTCGA`	`49 bp deleted → frameshift`
30	`GGCTGAGAAGAGGA----------------------------------------------`	`46 bp deleted → frameshift`
25	`GGCTGAGAAGA-------------------------------------------------`	`49 bp deleted → frameshift`
23	`GGCTG---------------------------------------------------TCGA`	`51 bp deleted → no frameshift`
22	`GGCTGAGA----------------------------------------------------`	`52 bp deleted → frameshift`
20	`GGCTGA------------------------------------------------------`	`54 bp deleted → no frameshift`

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