CRISPOR

DNA break repair outcome predictions

Guide: GCCTCCCCCGCCACGCTCCC GGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

mh- Score	Sequence	Effect
	`TCGACCGGCTCCTCCTCCTGGCCTCCCCCGCCACGCTCCCGGGAAGTCGGAGGGCCGGCTCGCGGGCTCAGGCAACCGTG`	(Wild-type sequence)
221	`CCTCCTCCTGGCCTCCCCCGCCACGCTC-----------------------GCGGGCTCA`	`23 bp deleted → frameshift`
198	`CCTCCTCCTGGCCTCCCCCGCCACG--------------GAGGGCCGGCTCGCGGGCTCA`	`14 bp deleted → frameshift`
182	`CCTCCTCCTGGCCTCCCCCGCCACGCTC----------GGAGGGCCGGCTCGCGGGCTCA`	`10 bp deleted → frameshift`
180	`CCTCCTCCTGGCCTCCCCCGCC------------------------GGCTCGCGGGCTCA`	`24 bp deleted → no frameshift`
179	`CCTCCTCCTGGCCTCCCCCGCCACGCTCCC----------------GGCTCGCGGGCTCA`	`16 bp deleted → frameshift`
170	`CCTCCTCCTGGCCTCCCCCGCCACGCTCC-----------------GGCTCGCGGGCTCA`	`17 bp deleted → frameshift`
155	`CCTCCTCCTGGCCTCCCCCGCCACGC---------------------------GGGCTCA`	`27 bp deleted → no frameshift`
155	`CCTCCTCCTGGCCTCCCCCG---------------------------GCTCGCGGGCTCA`	`27 bp deleted → no frameshift`
154	`CCTCCTCCTGGCCTCCCCCGCCACGC-------------------CGGCTCGCGGGCTCA`	`19 bp deleted → frameshift`
154	`CCTCCTCCTGGCCTCCCCCG-------------------GAGGGCCGGCTCGCGGGCTCA`	`19 bp deleted → frameshift`
153	`CCTCCTCCTGGCC---------------------------------GGCTCGCGGGCTCA`	`33 bp deleted → no frameshift`
148	`CCTCCTCCTGGCCTCCCCCGCCACGCTC-------------------------------A`	`31 bp deleted → frameshift`
140	`CCTCCTCCTGG---------------------GAAGTCGGAGGGCCGGCTCGCGGGCTCA`	`21 bp deleted → no frameshift`
133	`CCTCCTCCTGGCCTCCCCCGCCACG----------------------GCTCGCGGGCTCA`	`22 bp deleted → frameshift`
133	`CCTCCTCCTGG----------------------AAGTCGGAGGGCCGGCTCGCGGGCTCA`	`22 bp deleted → frameshift`
121	`CCTCCTCCTGGCCTCCCCCGC--------------------------------GGGCTCA`	`32 bp deleted → frameshift`
98	`CCTCCTCCTGGCCTCCCCCGC----------------------------TCGCGGGCTCA`	`28 bp deleted → frameshift`
98	`CCTCCTCCTGGCCTCCCC----------------------------GGCTCGCGGGCTCA`	`28 bp deleted → frameshift`
95	`CCTCCTCCTGGCCTC-----------------------GGAGGGCCGGCTCGCGGGCTCA`	`23 bp deleted → frameshift`
94	`CCTCCTCCTGGC-------------------------------------TCGCGGGCTCA`	`37 bp deleted → frameshift`
94	`CCTCCTCCTGGCCTCCCCCGCCACG-----------------------------GGCTCA`	`29 bp deleted → frameshift`
94	`CCTCCTCCTGGCCTCCC-----------------------------GGCTCGCGGGCTCA`	`29 bp deleted → frameshift`
94	`CCTCCTCCTGG-----------------------------AGGGCCGGCTCGCGGGCTCA`	`29 bp deleted → frameshift`
89	`CCTCCTCCTGGCCTCC------------------------------GGCTCGCGGGCTCA`	`30 bp deleted → no frameshift`
82	`CCTCCTCCTGGCCTC------------------------------------GCGGGCTCA`	`36 bp deleted → no frameshift`
80	`CCTCCTCCTGG--------------------------------GCCGGCTCGCGGGCTCA`	`32 bp deleted → frameshift`
73	`CCTCCTCCTGGCCTCCCCCG----------------------------------GGCTCA`	`34 bp deleted → frameshift`
66	`CCTCCTCCTGGCCTCCCCCGC------------------------------------TCA`	`36 bp deleted → no frameshift`
63	`CCTCCTC-------------------------------GGAGGGCCGGCTCGCGGGCTCA`	`31 bp deleted → frameshift`
63	`CCTCCTCCTGGC---------------------------------------------TCA`	`45 bp deleted → no frameshift`
60	`CCTCCTCC--------------------------------------GGCTCGCGGGCTCA`	`38 bp deleted → frameshift`
55	`CCTCCTCCTGGCCTC--------------------------------------------A`	`44 bp deleted → frameshift`
55	`CCTCCTC--------------------------------------------GCGGGCTCA`	`44 bp deleted → frameshift`
54	`CCTC----------------------------------GGAGGGCCGGCTCGCGGGCTCA`	`34 bp deleted → frameshift`
51	`CCTCCTCCTGGC-----------------------------------------GGGCTCA`	`41 bp deleted → frameshift`
51	`CCTCC-----------------------------------------GGCTCGCGGGCTCA`	`41 bp deleted → frameshift`
47	`CCTC-----------------------------------------------GCGGGCTCA`	`47 bp deleted → frameshift`
47	`CCTCCTCCTGGCCTCCCCCGCCA-------------------------------------`	`37 bp deleted → frameshift`
44	`CCTCCTCCTGG--------------------------------------------GCTCA`	`44 bp deleted → frameshift`
44	`CC--------------------------------------------GGCTCGCGGGCTCA`	`44 bp deleted → frameshift`
38	`CCTCCTCCT-----------------------------------------CGCGGGCTCA`	`41 bp deleted → frameshift`
37	`CCTCCTC----------------------------------------------------A`	`52 bp deleted → frameshift`
32	`CCTC-------------------------------------------------------A`	`55 bp deleted → frameshift`
25	`CCTCCTCCT-------------------------------------------------CA`	`49 bp deleted → frameshift`

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