CRISPOR

DNA break repair outcome predictions

Guide: CTCTTCTCAGCCAAGATCCC AGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

mh- Score	Sequence	Effect
	`CTGCCTTGTCGCCTTAGCTCCTCTTCTCAGCCAAGATCCCAGGGAGCCTGGGATTAGGAGCTTACTTGGGGGTTTTCCCC`	(Wild-type sequence)
314	`GCCTTAGCTCCTCTTCTCAGCC----------------TGGGATTAGGAGCTTACTTGGG`	`16 bp deleted → frameshift`
268	`GCCTTAGCTCCTCTTCTCAGCCAAGATCCC--------TGGGATTAGGAGCTTACTTGGG`	`8 bp deleted → frameshift`
255	`GCCTTAGCTCCTCTTCTCAGCCAAGATCC---------TGGGATTAGGAGCTTACTTGGG`	`9 bp deleted → no frameshift`
211	`GCCTTAGCTCCTCTTCTCAGCCAAG-------GGAGCCTGGGATTAGGAGCTTACTTGGG`	`7 bp deleted → frameshift`
191	`GCCTTAGCTCCTCTTCTCAGCCAAGA---------GCCTGGGATTAGGAGCTTACTTGGG`	`9 bp deleted → no frameshift`
173	`GCCTTAGCTCCTCTTCTCAGCCAAG-----------CCTGGGATTAGGAGCTTACTTGGG`	`11 bp deleted → frameshift`
170	`GCCTTAGCTCCTCTTCTCAGCCAAGAT-----------------TAGGAGCTTACTTGGG`	`17 bp deleted → frameshift`
164	`GCCTTAGCTCCTCTTCTCAG------------GGAGCCTGGGATTAGGAGCTTACTTGGG`	`12 bp deleted → no frameshift`
129	`GCCTTAGCTCCT---------------------------GGGATTAGGAGCTTACTTGGG`	`27 bp deleted → no frameshift`
121	`GCCT-----------------------------------GGGATTAGGAGCTTACTTGGG`	`35 bp deleted → frameshift`
117	`GCCTTAGC-----------------------------CTGGGATTAGGAGCTTACTTGGG`	`29 bp deleted → frameshift`
111	`GCCTTAGCTCCTCTTCTCAGC------------------------------TTACTTGGG`	`30 bp deleted → no frameshift`
99	`GCCTTAGCTCCTCTTCTCAGCCAAG----------------------GAGCTTACTTGGG`	`22 bp deleted → frameshift`
99	`GCCTTAGCTCCTCTTCT----------------------GGGATTAGGAGCTTACTTGGG`	`22 bp deleted → frameshift`
95	`GCCTTAGCTCCTCTTCTCAGCCAAGA-----------------------GCTTACTTGGG`	`23 bp deleted → frameshift`
86	`GCCTTAGCTCCTCTTCTCAGCCAAG-------------------------CTTACTTGGG`	`25 bp deleted → frameshift`
86	`GCCTTAGCTCCTCT-------------------------GGGATTAGGAGCTTACTTGGG`	`25 bp deleted → frameshift`
86	`GCCTTAG-------------------------GGAGCCTGGGATTAGGAGCTTACTTGGG`	`25 bp deleted → frameshift`
77	`GCCTTAGCTCCTCTTCTCAG---------------------------GAGCTTACTTGGG`	`27 bp deleted → no frameshift`
69	`GCCTTAGCT-------------------------------------------TACTTGGG`	`43 bp deleted → frameshift`
67	`GCCTTAG----------------------------------------GAGCTTACTTGGG`	`40 bp deleted → frameshift`
66	`GCCTTAGCT------------------------------GGGATTAGGAGCTTACTTGGG`	`30 bp deleted → no frameshift`
60	`GCCTTAGCTCCTCTT--------------------------------------ACTTGGG`	`38 bp deleted → frameshift`
52	`GCCTTAGCTCCTCTTCT-----------------------------------TACTTGGG`	`35 bp deleted → frameshift`
48	`GCCTTAGCTCCTCTT------------------------------------------GGG`	`42 bp deleted → no frameshift`
45	`GCCTTA------------------------------------------------CTTGGG`	`48 bp deleted → no frameshift`
44	`GCCTTAGCTCCTCTT------------------------------AGGAGCTTACTTGGG`	`30 bp deleted → no frameshift`
42	`GCCTTAGCTCCTCTTCT---------------------------------------TGGG`	`39 bp deleted → no frameshift`
40	`GCCTTAGCTCCT----------------------------------------TACTTGGG`	`40 bp deleted → frameshift`
34	`GC-------------------------------------------------TTACTTGGG`	`49 bp deleted → frameshift`
33	`GCCTTAGCTCCT--------------------------------------------TGGG`	`44 bp deleted → frameshift`
29	`GCCTT----------------------------------------------------GGG`	`52 bp deleted → frameshift`
28	`GCCTTAGCT-----------------------------------------------TGGG`	`47 bp deleted → frameshift`

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