CRISPOR

DNA break repair outcome predictions

Guide: ATGACAGCTTAGAAAGAAGA GGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

mh- Score	Sequence	Effect
	`ATGACGATTGCAGCAGGAGGATGACAGCTTAGAAAGAAGAGGGCAATGGGGCTTCCTCCCAGAGGCGGTGCGGCACAGAG`	(Wild-type sequence)
180	`CAGCAGGAGGATGACAGCTT------------------------CCTCCCAGAGGCGGTG`	`24 bp deleted → no frameshift`
179	`CAGCAGGAGGATGACAGC----------------AATGGGGCTTCCTCCCAGAGGCGGTG`	`16 bp deleted → frameshift`
134	`CAGCAGGAGGATGACAGCTTAGAAAGAA--------TGGGGCTTCCTCCCAGAGGCGGTG`	`8 bp deleted → frameshift`
133	`CAGCAGGAGG----------------------GCAATGGGGCTTCCTCCCAGAGGCGGTG`	`22 bp deleted → frameshift`
126	`CAGCAGGAGGATGACAGCTTAGAAAGAAGA-----------------------GGCGGTG`	`23 bp deleted → frameshift`
126	`CAGCAGGAGG-----------------------CAATGGGGCTTCCTCCCAGAGGCGGTG`	`23 bp deleted → frameshift`
116	`CAGCAGGAGGATGACA-------------------ATGGGGCTTCCTCCCAGAGGCGGTG`	`19 bp deleted → frameshift`
115	`CAGCAGGAGGATGACAGCTTAGAAA-----------TGGGGCTTCCTCCCAGAGGCGGTG`	`11 bp deleted → frameshift`
114	`CAGCAGGAGGATG-------------------------GGGCTTCCTCCCAGAGGCGGTG`	`25 bp deleted → frameshift`
114	`CAGCAGG-------------------------GCAATGGGGCTTCCTCCCAGAGGCGGTG`	`25 bp deleted → frameshift`
111	`CAGCA------------------------------ATGGGGCTTCCTCCCAGAGGCGGTG`	`30 bp deleted → no frameshift`
109	`CAGCAGGAGGATGACAGCTTAGAA------------TGGGGCTTCCTCCCAGAGGCGGTG`	`12 bp deleted → no frameshift`
109	`CAGCAGGAGGATGACAGCTTAGAAAGA--------------------------GGCGGTG`	`26 bp deleted → frameshift`
109	`CAGCAGG--------------------------CAATGGGGCTTCCTCCCAGAGGCGGTG`	`26 bp deleted → frameshift`
94	`CAGCAGGAGG-----------------------------GGCTTCCTCCCAGAGGCGGTG`	`29 bp deleted → frameshift`
89	`CAGCAGGAGGATGACAGCTTAGA------------------------------GGCGGTG`	`30 bp deleted → no frameshift`
89	`CAGCAGGAGG------------------------------GCTTCCTCCCAGAGGCGGTG`	`30 bp deleted → no frameshift`
87	`CAGCAGGAGGATGACAG-----------------------------------AGGCGGTG`	`35 bp deleted → frameshift`
86	`CAGCAGGAGGATGACAGCTTAGAAAGAAG-------------------------GCGGTG`	`25 bp deleted → frameshift`
84	`CAGCAGGAGG-------------------------------CTTCCTCCCAGAGGCGGTG`	`31 bp deleted → frameshift`
80	`CAGCAGG--------------------------------GGCTTCCTCCCAGAGGCGGTG`	`32 bp deleted → frameshift`
76	`CAGCAGG---------------------------------GCTTCCTCCCAGAGGCGGTG`	`33 bp deleted → no frameshift`
74	`CAGCAGGAGGATGACAGCTTAGAAAG----------------------------GCGGTG`	`28 bp deleted → frameshift`
74	`CAGCAGGAGGATGACAGCT----------------------------CCCAGAGGCGGTG`	`28 bp deleted → frameshift`
73	`CAGCAGGAGG---------------------------------------------CGGTG`	`45 bp deleted → no frameshift`
73	`CAGCAGG----------------------------------CTTCCTCCCAGAGGCGGTG`	`34 bp deleted → frameshift`
60	`CAGCAGGAGGATGACAGCTTAG--------------------------------GCGGTG`	`32 bp deleted → frameshift`
60	`CAGCAGGAGGATGACAGC--------------------------------------GGTG`	`38 bp deleted → frameshift`
60	`CAGC--------------------------------------TTCCTCCCAGAGGCGGTG`	`38 bp deleted → frameshift`
57	`CA---------------------------------ATGGGGCTTCCTCCCAGAGGCGGTG`	`33 bp deleted → no frameshift`
50	`CAGCAG----------------------------------------------AGGCGGTG`	`46 bp deleted → frameshift`
47	`CAGCAGGAGGATGACAG-------------------------------------GCGGTG`	`37 bp deleted → frameshift`
45	`CAGCAGG------------------------------------------------CGGTG`	`48 bp deleted → no frameshift`
43	`CAG-------------------------------------------------AGGCGGTG`	`49 bp deleted → frameshift`
42	`CAGCAGGAGGATGA---------------------------------------GGCGGTG`	`39 bp deleted → no frameshift`
36	`CAGCAGGAGGA------------------------------------------GGCGGTG`	`42 bp deleted → no frameshift`
36	`CAGCAGGAGG------------------------------------------------TG`	`48 bp deleted → no frameshift`
34	`CAGCAGGAG-------------------------------------------AGGCGGTG`	`43 bp deleted → frameshift`
31	`CAGCAGG---------------------------------------------------TG`	`51 bp deleted → no frameshift`
29	`CAGC----------------------------------------------------GGTG`	`52 bp deleted → frameshift`
28	`CAGCAGGAGGATG-----------------------------------------------`	`47 bp deleted → frameshift`
23	`CAG---------------------------------------------------GCGGTG`	`51 bp deleted → no frameshift`

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