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The following table lists possible deletions.
Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.
| mh- Score | Sequence | Effect |
|---|---|---|
| TTCTATTTATCCACTTGCGTTCGCCGAGTGGCGTCACCAGCGGTACTGTAATGACGATTGCAGCAGGAGGATGACAGCTT | (Wild-type sequence) | |
| 255 | CCACTTGCGTTCGCCGAGTGGCG---------GTACTGTAATGACGATTGCAGCAGGAGG | 9 bp deleted → no frameshift |
| 219 | CCACTTGCGTTCGCCGAGTGG------------TACTGTAATGACGATTGCAGCAGGAGG | 12 bp deleted → no frameshift |
| 191 | CCACTTGCGTTCGCCGAGTGGCGTCAC---------TGTAATGACGATTGCAGCAGGAGG | 9 bp deleted → no frameshift |
| 182 | CCACTTGCGTTCGCCGAGTGGCGT----------ACTGTAATGACGATTGCAGCAGGAGG | 10 bp deleted → frameshift |
| 179 | CCACTTGCGTTCGCCG----------------GTACTGTAATGACGATTGCAGCAGGAGG | 16 bp deleted → frameshift |
| 158 | CCACTTGCGTTCGCCGAGTGGCGTCACCAG-----------------------CAGGAGG | 23 bp deleted → frameshift |
| 154 | CCACTTGCGTTCG-------------------GTACTGTAATGACGATTGCAGCAGGAGG | 19 bp deleted → frameshift |
| 141 | CCACTTGCGTTCGCCGAGTGGCGT---------------AATGACGATTGCAGCAGGAGG | 15 bp deleted → no frameshift |
| 141 | CCACTTGCGTTCGCCGAGT---------------ACTGTAATGACGATTGCAGCAGGAGG | 15 bp deleted → no frameshift |
| 136 | CCACTTGCGTTCGCCGAGTGGCGTCACCAG--------------------------GAGG | 26 bp deleted → frameshift |
| 126 | CCACTTGCGTTCGCCGAGTGGCG-----------------------ATTGCAGCAGGAGG | 23 bp deleted → frameshift |
| 126 | CCACTTGCG-----------------------GTACTGTAATGACGATTGCAGCAGGAGG | 23 bp deleted → frameshift |
| 122 | CCACTTGCGTTCGCCGAGTGGCGTCAC------------------GATTGCAGCAGGAGG | 18 bp deleted → no frameshift |
| 122 | CCACTTGCGTTCGCCGAGTG------------------TAATGACGATTGCAGCAGGAGG | 18 bp deleted → no frameshift |
| 111 | CCACTTGCGTTCGCCGA------------------------------TTGCAGCAGGAGG | 30 bp deleted → no frameshift |
| 110 | CCACTTGCGTTCGCCGAGT--------------------AATGACGATTGCAGCAGGAGG | 20 bp deleted → frameshift |
| 95 | CCACTTGCGTTCGCCGAGTG-----------------------ACGATTGCAGCAGGAGG | 23 bp deleted → frameshift |
| 94 | CCACTTGCGTTCGCCGAGTGGC-----------------------------AGCAGGAGG | 29 bp deleted → frameshift |
| 90 | CCACTTGCGT------------------------ACTGTAATGACGATTGCAGCAGGAGG | 24 bp deleted → no frameshift |
| 81 | CCACTTGCGTTCGCCGAGTGGCGTCA--------------------------GCAGGAGG | 26 bp deleted → frameshift |
| 80 | CCACTTGCGTTCGCCGAGTGGC--------------------------------AGGAGG | 32 bp deleted → frameshift |
| 80 | CCACT--------------------------------GTAATGACGATTGCAGCAGGAGG | 32 bp deleted → frameshift |
| 77 | CCACTTGCGTTCGCCGA---------------------------CGATTGCAGCAGGAGG | 27 bp deleted → no frameshift |
| 76 | CCACTTGCGTTCG---------------------------------ATTGCAGCAGGAGG | 33 bp deleted → no frameshift |
| 70 | CCACTTGCGTTCGCCGAGTGGCGTCACCAG-----------------------------G | 29 bp deleted → frameshift |
| 70 | CCACTTGCGTTCGCCGAGTGGCGTCA-----------------------------GGAGG | 29 bp deleted → frameshift |
| 70 | CCACTTGCGT-----------------------------AATGACGATTGCAGCAGGAGG | 29 bp deleted → frameshift |
| 69 | CCACTTGC-------------------------------------------AGCAGGAGG | 43 bp deleted → frameshift |
| 66 | CCACTTGCGTTCGCCGAGTG------------------------------CAGCAGGAGG | 30 bp deleted → no frameshift |
| 66 | CCACTTGCGTTCGCCGAGTGG------------------------------------AGG | 36 bp deleted → no frameshift |
| 64 | CCACTTGCGTTCGCCGAG-----------------------------------------G | 41 bp deleted → frameshift |
| 63 | CCACTTG-------------------------------TAATGACGATTGCAGCAGGAGG | 31 bp deleted → frameshift |
| 62 | CCACTTGCGTTCGC-------------------------------------AGCAGGAGG | 37 bp deleted → frameshift |
| 62 | CCACTTGCG-------------------------------------ATTGCAGCAGGAGG | 37 bp deleted → frameshift |
| 56 | CCACTTGCGTTCGCCGAGTGG--------------------------------------- | 39 bp deleted → no frameshift |
| 54 | CCACTTGCGTTCGC----------------------------------------AGGAGG | 40 bp deleted → frameshift |
| 52 | CCACTTGCGTTCGCCGAG-----------------------------------CAGGAGG | 35 bp deleted → frameshift |
| 49 | CCACTTG------------------------------------ACGATTGCAGCAGGAGG | 36 bp deleted → no frameshift |
| 45 | CCACTTGCGTTCGCCGAG--------------------------------------GAGG | 38 bp deleted → frameshift |
| 40 | CCACTTGC----------------------------------------------AGGAGG | 46 bp deleted → frameshift |
| 38 | CCAC-----------------------------------------GATTGCAGCAGGAGG | 41 bp deleted → frameshift |
| 30 | CCACTTGCGTT--------------------------------------GCAGCAGGAGG | 38 bp deleted → frameshift |
| 25 | CCA-------------------------------------------------GCAGGAGG | 49 bp deleted → frameshift |
| 22 | CCA----------------------------------------------------GGAGG | 52 bp deleted → frameshift |