CRISPOR

DNA break repair outcome predictions

Guide: CGAGCCACGGTCTGCTGGAG CGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

mh- Score	Sequence	Effect
	`AGTGCTGCAGGGCTGGGCGCCGAGCCACGGTCTGCTGGAGCGGCCATGCTTGGCCCGGGACCCCCAGTCCCCTCCGCGGA`	(Wild-type sequence)
313	`GGCTGGGCGCCGAGCCACGG-------------CCATGCTTGGCCCGGGACCCCCAGTCC`	`13 bp deleted → frameshift`
311	`GGCTGGGCGCCGAGCCACGGTCTGCTGG-----CCATGCTTGGCCCGGGACCCCCAGTCC`	`5 bp deleted → frameshift`
298	`GGCTGGGCGCCGAGCCACGGTCTGCT--------------TGGCCCGGGACCCCCAGTCC`	`14 bp deleted → frameshift`
270	`GGCTGGGCGCCGAGCCA-------------------TGCTTGGCCCGGGACCCCCAGTCC`	`19 bp deleted → frameshift`
255	`GGCTGGGCGCCGAGCCACGGTCTGC---------CATGCTTGGCCCGGGACCCCCAGTCC`	`9 bp deleted → no frameshift`
235	`GGCTGGGCGCCGAGCCACGGTCTGCTGG---------------CCCGGGACCCCCAGTCC`	`15 bp deleted → no frameshift`
180	`GGCTGGGCGCC------------------------ATGCTTGGCCCGGGACCCCCAGTCC`	`24 bp deleted → no frameshift`
175	`GGCTGGGCGCCGAGCCACGGTCTGCTGGA---------------------CCCCCAGTCC`	`21 bp deleted → no frameshift`
173	`GGCTGGGCGCCGAGCCACGGTCTGCTG-----------CTTGGCCCGGGACCCCCAGTCC`	`11 bp deleted → frameshift`
163	`GGCTGGGC--------------------------CATGCTTGGCCCGGGACCCCCAGTCC`	`26 bp deleted → frameshift`
154	`GGCTGGGCGCCGAGCCACGGTCTGC-------------------CCGGGACCCCCAGTCC`	`19 bp deleted → frameshift`
148	`GGCTGGGCGCCGAGCCACGG----------------------------GACCCCCAGTCC`	`28 bp deleted → frameshift`
147	`GGCTGGGCGCCGAGCCACGGTCTGCTGG--------------------GACCCCCAGTCC`	`20 bp deleted → frameshift`
147	`GGCTGGGCGCCG--------------------GCCATGCTTGGCCCGGGACCCCCAGTCC`	`20 bp deleted → frameshift`
141	`GGCTGGGCGCCGAGCC-----------------------------CGGGACCCCCAGTCC`	`29 bp deleted → frameshift`
128	`GGCTGGGCGCCGAGCCACGGTCT-----------------TGGCCCGGGACCCCCAGTCC`	`17 bp deleted → frameshift`
127	`GGC-------------------------------CATGCTTGGCCCGGGACCCCCAGTCC`	`31 bp deleted → frameshift`
126	`GGCTGGGCGCCGAGCCACGG-----------------------CCCGGGACCCCCAGTCC`	`23 bp deleted → frameshift`
126	`GGCTGGGCG-----------------------GCCATGCTTGGCCCGGGACCCCCAGTCC`	`23 bp deleted → frameshift`
122	`GGCTGGGCGCCGAGCCACGGTCTG------------------GCCCGGGACCCCCAGTCC`	`18 bp deleted → no frameshift`
120	`GGCTGGGCGCCGAGC------------------------TTGGCCCGGGACCCCCAGTCC`	`24 bp deleted → no frameshift`
109	`GGCTGGGCGCC----------------------------------CGGGACCCCCAGTCC`	`34 bp deleted → frameshift`
104	`GGCTGGGCGCCG-----------------------------------GGACCCCCAGTCC`	`35 bp deleted → frameshift`
103	`GGCTGG---------------------------CCATGCTTGGCCCGGGACCCCCAGTCC`	`27 bp deleted → no frameshift`
99	`GGCTGGGC------------------------------------CCGGGACCCCCAGTCC`	`36 bp deleted → no frameshift`
94	`GGCTGGGCGCCGAGCCACGG-----------------------------ACCCCCAGTCC`	`29 bp deleted → frameshift`
94	`GGCTGGGCGC-----------------------------TTGGCCCGGGACCCCCAGTCC`	`29 bp deleted → frameshift`
89	`GGCTGGGCGCCGAGCC------------------------------GGGACCCCCAGTCC`	`30 bp deleted → no frameshift`
84	`GGCTGGGC-------------------------------TTGGCCCGGGACCCCCAGTCC`	`31 bp deleted → frameshift`
82	`GGCT------------------------------------TGGCCCGGGACCCCCAGTCC`	`36 bp deleted → no frameshift`
78	`GGCTGGGCGCCGAGCCACGGTC-------------------------------------C`	`37 bp deleted → frameshift`
78	`GGCTGG-------------------------------------CCCGGGACCCCCAGTCC`	`37 bp deleted → frameshift`
77	`GGCTGGGCGCCGAGCCACGGTCTGCTGGAG---------------------------TCC`	`27 bp deleted → no frameshift`
77	`GGC-----------------------------------------CCGGGACCCCCAGTCC`	`41 bp deleted → frameshift`
73	`GGCTGGG------------------------------------------ACCCCCAGTCC`	`42 bp deleted → no frameshift`
71	`GGCTGGGCGCCGAGCCA---------------------------------------GTCC`	`39 bp deleted → no frameshift`
66	`GGCTGGGCGCCGAGCC------------------------------------CCCAGTCC`	`36 bp deleted → no frameshift`
62	`GGCTGGGCGCCGAGCC-------------------------------------CCAGTCC`	`37 bp deleted → frameshift`
60	`GGCTGGGCGCCGAGCC--------------------------------------CAGTCC`	`38 bp deleted → frameshift`
60	`GGCTGGGCG--------------------------------------GGACCCCCAGTCC`	`38 bp deleted → frameshift`
57	`GGCTGGGCGCCGAGCCAC---------------------------------CCCCAGTCC`	`33 bp deleted → no frameshift`
57	`GGCTG---------------------------------CTTGGCCCGGGACCCCCAGTCC`	`33 bp deleted → no frameshift`
51	`GGCTGGGCGCC-----------------------------------------CCCAGTCC`	`41 bp deleted → frameshift`
51	`GGCTGGG-----------------------------------------GACCCCCAGTCC`	`41 bp deleted → frameshift`
48	`GGCTGGGCGCC------------------------------------------CCAGTCC`	`42 bp deleted → no frameshift`
47	`GGCTGGGCGCCGA-------------------------------------CCCCCAGTCC`	`37 bp deleted → frameshift`
46	`GGCTGGGCGCC-------------------------------------------CAGTCC`	`43 bp deleted → frameshift`
46	`GGCTGG-------------------------------------------ACCCCCAGTCC`	`43 bp deleted → frameshift`
44	`GGCTGGGCGCCGAGCC--------------------------------------------`	`44 bp deleted → frameshift`
44	`GGCTGGGCGCC--------------------------------------------AGTCC`	`44 bp deleted → frameshift`
40	`GG----------------------------------------------GACCCCCAGTCC`	`46 bp deleted → frameshift`
38	`GG-----------------------------------------------ACCCCCAGTCC`	`47 bp deleted → frameshift`
34	`GGCTGGGCGCCGAG-------------------------------------------TCC`	`43 bp deleted → frameshift`
34	`GGCTGGGCGCC-------------------------------------------------`	`49 bp deleted → frameshift`

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