CRISPOR

DNA break repair outcome predictions

Guide: CAGCGGGCTGCTCACGGCTT CGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

mh- Score	Sequence	Effect
	`ATCTTCTCTGGCGGTTCAGGCAGCGGGCTGCTCACGGCTTCGGACAAATTAGAGACCCTCTTACCAACCAGAGTGCCAAG`	(Wild-type sequence)
444	`GCGGTTCAGGCAGCGGGCTGCTCACGG------ACAAATTAGAGACCCTCTTACCAACCA`	`6 bp deleted → no frameshift`
256	`GCGGTTCAGGCAGCGG-----------------ACAAATTAGAGACCCTCTTACCAACCA`	`17 bp deleted → frameshift`
182	`GCGGTTCAGGCAGCGGGCTGCTCAC----------AAATTAGAGACCCTCTTACCAACCA`	`10 bp deleted → frameshift`
179	`GCGGTTCAGGCAGCGGG----------------ACAAATTAGAGACCCTCTTACCAACCA`	`16 bp deleted → frameshift`
164	`GCGGTTCAGGCAGCGGGCTGCTCA------------AATTAGAGACCCTCTTACCAACCA`	`12 bp deleted → no frameshift`
141	`GCGG-----------------------------ACAAATTAGAGACCCTCTTACCAACCA`	`29 bp deleted → frameshift`
133	`GCGGTTCAGGCAGCGGGCTGCTCACGGCTT----------------------ACCAACCA`	`22 bp deleted → frameshift`
129	`GCGGTTCAGGCAGCGGGCTGCTC---------------------------TTACCAACCA`	`27 bp deleted → no frameshift`
126	`GCGGTTCAGG-----------------------ACAAATTAGAGACCCTCTTACCAACCA`	`23 bp deleted → frameshift`
121	`GCGGTTCAGGCAGCGGGCTGCTCACGGCTT----------AGAGACCCTCTTACCAACCA`	`10 bp deleted → frameshift`
110	`GCGGTTCAGGCAGCGGGCTGCTCACGGCT--------------------CTTACCAACCA`	`20 bp deleted → frameshift`
105	`GCGGTTCAGGCAGCGGGCTGCTCAC---------------------CCTCTTACCAACCA`	`21 bp deleted → no frameshift`
90	`GCGGTTCAGGCA------------------------AATTAGAGACCCTCTTACCAACCA`	`24 bp deleted → no frameshift`
74	`GCGGTTCA----------------------------AATTAGAGACCCTCTTACCAACCA`	`28 bp deleted → frameshift`
70	`GCGGTTCAGGCAGCGGGCTGCTCAC-----------------------------CAACCA`	`29 bp deleted → frameshift`
70	`GCGGTTCAGGCAGCGGGCTGCT-----------------------------TACCAACCA`	`29 bp deleted → frameshift`
70	`GCGGTTCAGGCAG-----------------------------AGACCCTCTTACCAACCA`	`29 bp deleted → frameshift`
66	`GCGGTTCAGGCAGCGGGCT------------------------------CTTACCAACCA`	`30 bp deleted → no frameshift`
63	`GCGGTTCAGGCAG-------------------------------ACCCTCTTACCAACCA`	`31 bp deleted → frameshift`
60	`GCGGTTCAGGCAGCGGGCTGCTCA--------------------------------ACCA`	`32 bp deleted → frameshift`
60	`GCGGTTCAGGCAGCGGGCT--------------------------------TACCAACCA`	`32 bp deleted → frameshift`
57	`GCGGTTCAGGCAGCGGGCTGCTCAC---------------------------------CA`	`33 bp deleted → no frameshift`
57	`GCGGTTCAG---------------------------------AGACCCTCTTACCAACCA`	`33 bp deleted → no frameshift`
52	`GCGGTTCAG-----------------------------------ACCCTCTTACCAACCA`	`35 bp deleted → frameshift`
49	`GCGGTTCAGGCAGCGGGCTGCTCA------------------------------------`	`36 bp deleted → no frameshift`
36	`GCGGTT----------------------------------AGAGACCCTCTTACCAACCA`	`34 bp deleted → frameshift`
34	`GCGGTTC-------------------------------------------TTACCAACCA`	`43 bp deleted → frameshift`
33	`GCGGTTCAGGCA--------------------------------------------ACCA`	`44 bp deleted → frameshift`
27	`GCGGTTCAGGCA------------------------------------------------`	`48 bp deleted → no frameshift`
27	`GCGGTTCA------------------------------------------------ACCA`	`48 bp deleted → no frameshift`
22	`GCGGTTCA----------------------------------------------------`	`52 bp deleted → frameshift`
20	`GCGGTT----------------------------------------------ACCAACCA`	`46 bp deleted → frameshift`

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