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Contents:In the table below, Illumina Nextera Handle sequences have been added and highlighted in bold. Primers for the on-target have been added for convenience. The table below is sorted by the CFD off-target score. Sites with very low CFD scores < 0.02 are unlikely to be cleaved, see our study Haeussler et al. 2016, Figure 2.
In the protocol by Matthew Canver, Harvard, two PCRs are run: one PCR to amplify the potential off-target, then a second PCR to extend the handles with Illumina barcodes. Please click here to download the protocol. Alternatively, you can have a look at Fu et al, 2014.
If a primer was not found, the reason is usually that the region around the off-target is too repetitive. To avoid unspecific primers, all repeats are masked for the primer design (not for off-target search). If you think that we should change the parameters here or should use different primer3 settings, please let us know.
| Name | Primer Sequence | Tm | CFD Score |
|---|---|---|---|
| ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52314088_F | TCGTCGGCAGCGTCGTCGATGGACTAGCAGGCAG | 60.2 | 1.00 |
| ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52314088_R | GTCTCGTGGGCTCGGAGCTACTAGGTTGCCCTTGG | 59.0 | 1.00 |
| mm4_intergenic_Cyp1a1|Edc3_chr9_57704674_F | Primer3: not found at this Tm | N.d. | 0.61 |
| mm4_intergenic_Cyp1a1|Edc3_chr9_57704674_R | Primer3: not found at this Tm | N.d. | 0.61 |
| mm4_intron_Megf6_chr4_154231802_F | TCGTCGGCAGCGTCTGGTCCCAGTGATAGGTTGC | 59.3 | 0.58 |
| mm4_intron_Megf6_chr4_154231802_R | GTCTCGTGGGCTCGGAGGCAATGAGGAAGAAGGGC | 60.0 | 0.58 |
| mm4_intergenic_Gm11397|Serpinb9g_chr13_33430464_F | TCGTCGGCAGCGTCTCTGGACTGCAATGGTTCCC | 59.9 | 0.55 |
| mm4_intergenic_Gm11397|Serpinb9g_chr13_33430464_R | GTCTCGTGGGCTCGGGCATCTTTGCTTTAAGCCTTTTGA | 59.0 | 0.55 |
| mm4_intergenic_Gm22802|Slitrk5_chr14_111492823_F | Primer3: not found at this Tm | N.d. | 0.42 |
| mm4_intergenic_Gm22802|Slitrk5_chr14_111492823_R | Primer3: not found at this Tm | N.d. | 0.42 |
| mm3_intron_Ncf4_chr15_78253683_F | TCGTCGGCAGCGTCAGCACCCTGTCAACAGTAGC | 59.9 | 0.41 |
| mm3_intron_Ncf4_chr15_78253683_R | GTCTCGTGGGCTCGGGTCTCCAAAGCCAGAGCACT | 59.9 | 0.41 |
| mm4_intron_Atrnl1_chr19_57725038_F | Primer3: not found at this Tm | N.d. | 0.31 |
| mm4_intron_Atrnl1_chr19_57725038_R | Primer3: not found at this Tm | N.d. | 0.31 |
| mm4_intron_Unc13d_chr11_116072059_F | Primer3: not found at this Tm | N.d. | 0.29 |
| mm4_intron_Unc13d_chr11_116072059_R | Primer3: not found at this Tm | N.d. | 0.29 |
| mm4_intergenic_Gm22986|Adam26b_chr8_43472887_F | Primer3: not found at this Tm | N.d. | 0.28 |
| mm4_intergenic_Gm22986|Adam26b_chr8_43472887_R | Primer3: not found at this Tm | N.d. | 0.28 |
| mm4_intergenic_Serpinb9c|Serpinb9d_chr13_33186180_F | TCGTCGGCAGCGTCTCTGGACTGCAATGGTTCCC | 59.9 | 0.26 |
| mm4_intergenic_Serpinb9c|Serpinb9d_chr13_33186180_R | GTCTCGTGGGCTCGGTCTAAGGCATAAACTTCTTCTCAGC | 58.8 | 0.26 |
| mm4_intergenic_Atp6v1e2|Rhoq_chr17_86951454_F | Primer3: not found at this Tm | N.d. | 0.26 |
| mm4_intergenic_Atp6v1e2|Rhoq_chr17_86951454_R | Primer3: not found at this Tm | N.d. | 0.26 |
| mm4_intergenic_Acer2|Slc24a2_chr4_86941008_F | Primer3: not found at this Tm | N.d. | 0.23 |
| mm4_intergenic_Acer2|Slc24a2_chr4_86941008_R | Primer3: not found at this Tm | N.d. | 0.23 |
| mm2_intergenic_Rab27b|Dynap_chr18_70141623_F | TCGTCGGCAGCGTCAAACCGGCTCAGTCCTTCTG | 59.9 | 0.23 |
| mm2_intergenic_Rab27b|Dynap_chr18_70141623_R | GTCTCGTGGGCTCGGTGGCAGACACAGCAGATTGT | 59.8 | 0.23 |
| mm4_intron_Pcgf2_chr11_97699083_F | Primer3: not found at this Tm | N.d. | 0.22 |
| mm4_intron_Pcgf2_chr11_97699083_R | Primer3: not found at this Tm | N.d. | 0.22 |
| mm3_intron_Myo18a_chr11_77832606_F | TCGTCGGCAGCGTCCTAGACACTGCAGGCTGGTG | 60.3 | 0.21 |
| mm3_intron_Myo18a_chr11_77832606_R | GTCTCGTGGGCTCGGCCTCTCCAATCTGTGCTGCA | 60.0 | 0.21 |
| mm4_intergenic_Gm15405|Gm27039_chr17_92080915_F | TCGTCGGCAGCGTCCCCAACAAGCCCATTACCCT | 59.9 | 0.15 |
| mm4_intergenic_Gm15405|Gm27039_chr17_92080915_R | GTCTCGTGGGCTCGGTTGGATCTCAAGCCTGCAGG | 60.0 | 0.15 |
| mm4_intergenic_Gm26815|Gm26747_chr8_121377753_F | TCGTCGGCAGCGTCAGCTGGGTGGATTTACTGCC | 60.0 | 0.15 |
| mm4_intergenic_Gm26815|Gm26747_chr8_121377753_R | GTCTCGTGGGCTCGGTGCAGGGCAAGTGATTAGGG | 60.0 | 0.15 |
| mm4_intergenic_Cdh10|Gm25976_chr15_19757729_F | Primer3: not found at this Tm | N.d. | 0.15 |
| mm4_intergenic_Cdh10|Gm25976_chr15_19757729_R | Primer3: not found at this Tm | N.d. | 0.15 |
| mm4_intron_Acvr1_chr2_58550495_F | TCGTCGGCAGCGTCAGTCACAGGCTCAGCAACTG | 60.2 | 0.14 |
| mm4_intron_Acvr1_chr2_58550495_R | GTCTCGTGGGCTCGGAGGCTGGTGCTCTGTTTGTT | 60.1 | 0.14 |
| mm3_intron_Ptges2_chr2_32396827_F | TCGTCGGCAGCGTCCAGACTCAGAACTGGAGGCG | 60.1 | 0.14 |
| mm3_intron_Ptges2_chr2_32396827_R | GTCTCGTGGGCTCGGCAGGAAAGGAGCATGGGAGG | 60.1 | 0.14 |
| mm4_exon_Cdr2_chr7_120958425_F | TCGTCGGCAGCGTCCTGCTGCTTCGTTTGAGTGG | 59.7 | 0.13 |
| mm4_exon_Cdr2_chr7_120958425_R | GTCTCGTGGGCTCGGATGGTGTGGAGAAGCTGGTG | 59.9 | 0.13 |
| mm4_exon_Slc10a4_chr5_73007033_F | TCGTCGGCAGCGTCGACGCTTGACCAGACAGACA | 59.9 | 0.13 |
| mm4_exon_Slc10a4_chr5_73007033_R | GTCTCGTGGGCTCGGTGTTGTCCAGGCTGTCCATG | 60.2 | 0.13 |
| mm4_intergenic_1810011O10Rik/Gm26714|Ido2_chr8_24509203_F | TCGTCGGCAGCGTCAACAGCAGGGATGAGAACCG | 60.0 | 0.11 |
| mm4_intergenic_1810011O10Rik/Gm26714|Ido2_chr8_24509203_R | GTCTCGTGGGCTCGGAGAGGTGAGTGTGTGCTGAA | 58.8 | 0.11 |
| mm4_intron_Rnaseh1_chr12_28654193_F | TCGTCGGCAGCGTCCAACTGGGCCTGGCTAATCA | 60.0 | 0.11 |
| mm4_intron_Rnaseh1_chr12_28654193_R | GTCTCGTGGGCTCGGGGTTCTGAGGCCGGACAAAT | 60.3 | 0.11 |
| mm4_intergenic_Gm15606|Opcml_chr9_28399315_F | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intergenic_Gm15606|Opcml_chr9_28399315_R | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intergenic_Fscb|Gm24066_chr12_64588534_F | TCGTCGGCAGCGTCACCCCTTCTTTTCTCGTTGCT | 59.8 | 0.09 |
| mm4_intergenic_Fscb|Gm24066_chr12_64588534_R | GTCTCGTGGGCTCGGGCATAGGTACATTTCTGCTTCGA | 58.8 | 0.09 |
| mm4_intergenic_Arid4b|Gm26129_chr13_14065020_F | TCGTCGGCAGCGTCGAGTGGCTCGGTGTGGAG | 59.7 | 0.09 |
| mm4_intergenic_Arid4b|Gm26129_chr13_14065020_R | GTCTCGTGGGCTCGGCTGAACAGCTTGGGTGAGGT | 59.8 | 0.09 |
| mm4_intergenic_Fam78b|Uck2_chr1_167112007_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_Fam78b|Uck2_chr1_167112007_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intron_Esrrg_chr1_188095732_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intron_Esrrg_chr1_188095732_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_Stc2|Bod1_chr11_31466113_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_Stc2|Bod1_chr11_31466113_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intron_Lrpprc_chr17_84751000_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intron_Lrpprc_chr17_84751000_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm2_intron_Npas3_chr12_53562881_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm2_intron_Npas3_chr12_53562881_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intron_Tepp_chr8_95314178_F | TCGTCGGCAGCGTCGCTAGGAGCACCCTCTCTCT | 60.1 | 0.07 |
| mm4_intron_Tepp_chr8_95314178_R | GTCTCGTGGGCTCGGTGGCGTGTGAATCCAACCA | 59.8 | 0.07 |
| mm4_intergenic_Gm26137|Gm23733_chr3_120191336_F | TCGTCGGCAGCGTCTGAATGTCTGATCTTCTGATAGGGA | 58.8 | 0.06 |
| mm4_intergenic_Gm26137|Gm23733_chr3_120191336_R | GTCTCGTGGGCTCGGAGCATAGCAAGAAGAATGGAGCT | 60.1 | 0.06 |
| mm4_intergenic_Dbx2|Gm8849_chr15_95703024_F | TCGTCGGCAGCGTCAGGTGCCCCTTGCTTTGAAA | 60.3 | 0.06 |
| mm4_intergenic_Dbx2|Gm8849_chr15_95703024_R | GTCTCGTGGGCTCGGGTTCCAGAGCAGGGTCACAG | 60.3 | 0.06 |
| mm4_intergenic_Grap2|Fam83f_chr15_80665904_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_Grap2|Fam83f_chr15_80665904_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm2_intron_Hdhd2_chr18_76969410_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm2_intron_Hdhd2_chr18_76969410_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_exon_Slc4a8_chr15_100816052_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_exon_Slc4a8_chr15_100816052_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_A630010A05Rik|Snai2_chr16_14672195_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_A630010A05Rik|Snai2_chr16_14672195_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_Parp11|Efcab4b_chr6_127565667_F | TCGTCGGCAGCGTCTGTGATGGTGTGTCCTCGTG | 59.9 | 0.04 |
| mm4_intergenic_Parp11|Efcab4b_chr6_127565667_R | GTCTCGTGGGCTCGGACCCTGGCTTAGTTGTCACG | 59.9 | 0.04 |
| mm4_intergenic_Parp11|Efcab4b_chr6_127565597_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Parp11|Efcab4b_chr6_127565597_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intron_Cdh22_chr2_165183101_F | TCGTCGGCAGCGTCTTCCTGGCGCTTATGAGCTC | 60.1 | 0.04 |
| mm4_intron_Cdh22_chr2_165183101_R | GTCTCGTGGGCTCGGCCACACTTCCCTGTCCCTTC | 59.9 | 0.04 |
| mm4_exon_Pdxk_chr10_78440867_F | TCGTCGGCAGCGTCGTCCCTTTTGCTCTGGACCA | 59.8 | 0.04 |
| mm4_exon_Pdxk_chr10_78440867_R | GTCTCGTGGGCTCGGGCCTGCTCTGATCTTCCCTG | 60.1 | 0.04 |
| mm4_intron_E230016M11Rik_chr6_67072343_F | TCGTCGGCAGCGTCCCAACCAGTGAAGGTCGACA | 59.8 | 0.04 |
| mm4_intron_E230016M11Rik_chr6_67072343_R | GTCTCGTGGGCTCGGAAGAGCATGCCAAGCTGAGT | 59.9 | 0.04 |
| mm4_intron_Ttc21a_chr9_119957112_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intron_Ttc21a_chr9_119957112_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intron_Cacna1a_chr8_84552901_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intron_Cacna1a_chr8_84552901_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intron_Atoh8_chr6_72229939_F | TCGTCGGCAGCGTCTCCACTCCTCCTCTACTGCC | 60.0 | 0.03 |
| mm4_intron_Atoh8_chr6_72229939_R | GTCTCGTGGGCTCGGGAAAGGGGTCCTGTAAGGCC | 60.0 | 0.03 |
| mm4_intergenic_Ifng|Dyrk2/4932442E05Rik_chr10_118656624_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Ifng|Dyrk2/4932442E05Rik_chr10_118656624_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intron_Adck1_chr12_88445858_F | TCGTCGGCAGCGTCTTCCCAGGTGCAGAAACTGT | 59.4 | 0.03 |
| mm4_intron_Adck1_chr12_88445858_R | GTCTCGTGGGCTCGGGTGCTAAGAGCTGCTTCCCT | 59.7 | 0.03 |
| mm4_intron_Unc5d_chr8_28720637_F | TCGTCGGCAGCGTCACTCTAAGTCTGAGGGGCCA | 59.5 | 0.02 |
| mm4_intron_Unc5d_chr8_28720637_R | GTCTCGTGGGCTCGGCTGAGATGCTCCCACTTGCA | 60.0 | 0.02 |
| mm4_intergenic_Gm9992|Gm25872_chr17_7582772_F | TCGTCGGCAGCGTCTGCTCATCCGGATCCAAAGT | 59.0 | 0.02 |
| mm4_intergenic_Gm9992|Gm25872_chr17_7582772_R | GTCTCGTGGGCTCGGATCCCCACCCAACTCAGCTA | 60.2 | 0.02 |
| mm4_intron_Piwil4_chr9_14730521_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intron_Piwil4_chr9_14730521_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Cnnm2|Nt5c2_chr19_46879694_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Cnnm2|Nt5c2_chr19_46879694_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intron_D6Wsu163e_chr6_126963413_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intron_D6Wsu163e_chr6_126963413_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_exon_Baiap3_chr17_25245948_F | TCGTCGGCAGCGTCCCTCTCACCTGCTCACTCAC | 59.7 | 0.02 |
| mm4_exon_Baiap3_chr17_25245948_R | GTCTCGTGGGCTCGGACACGGAACTGCTGAGGAAG | 59.9 | 0.02 |
| mm4_intergenic_RP23-93M1.2|RP24-308I2.1_chr9_32886364_F | TCGTCGGCAGCGTCTGTCTGAACTGCCACAGTGC | 60.8 | 0.02 |
| mm4_intergenic_RP23-93M1.2|RP24-308I2.1_chr9_32886364_R | GTCTCGTGGGCTCGGGAAGTCCCCTGTCCAAAGCT | 59.5 | 0.02 |
| mm4_intron_Spock1_chr13_57541002_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_Spock1_chr13_57541002_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_Nav2_chr7_48998426_F | TCGTCGGCAGCGTCGTGGAGCAGAAAGGGACCTT | 59.5 | 0.01 |
| mm4_intron_Nav2_chr7_48998426_R | GTCTCGTGGGCTCGGCTGGCCAGACAGCTTCAGAG | 60.3 | 0.01 |
| mm4_intergenic_Svs5|Slpi/mmu-mir-7678_chr2_164339098_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Svs5|Slpi/mmu-mir-7678_chr2_164339098_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_Chn2_chr6_54040216_F | TCGTCGGCAGCGTCTGGAGAGTCTACCTGTGGCC | 60.6 | 0.01 |
| mm4_intron_Chn2_chr6_54040216_R | GTCTCGTGGGCTCGGGACGCAAAGGTGTTGACCAC | 59.9 | 0.01 |
| mm4_intergenic_Zfp516|Gm24720_chr18_83237038_F | TCGTCGGCAGCGTCTCTGTCTGCACTCACAGCTG | 59.9 | 0.01 |
| mm4_intergenic_Zfp516|Gm24720_chr18_83237038_R | GTCTCGTGGGCTCGGCCCTCGATGCTGAAGTCAGG | 60.1 | 0.01 |
| mm4_intergenic_Cdh4|Gm14300_chr2_179525563_F | TCGTCGGCAGCGTCCAAAGTGAGGGCAGGTCTGT | 59.8 | 0.01 |
| mm4_intergenic_Cdh4|Gm14300_chr2_179525563_R | GTCTCGTGGGCTCGGCTCACTCCAGGAGCACCAAC | 60.3 | 0.01 |
| mm4_intergenic_Gm7551|Gm20684_chr7_65803481_F | TCGTCGGCAGCGTCTGGCTTCATGCATGGTGTCT | 59.9 | 0.00 |
| mm4_intergenic_Gm7551|Gm20684_chr7_65803481_R | GTCTCGTGGGCTCGGCTGGCAAGATCATCAGCCCT | 59.8 | 0.00 |
| mm4_intergenic_4930506M07Rik|Vax1_chr19_59142678_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_4930506M07Rik|Vax1_chr19_59142678_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Lrp6_chr6_134468073_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Lrp6_chr6_134468073_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_AI606473|Tyw3_chr3_154540836_F | TCGTCGGCAGCGTCGGATATGACATCGCAGCCCA | 59.9 | 0.00 |
| mm4_intergenic_AI606473|Tyw3_chr3_154540836_R | GTCTCGTGGGCTCGGCCCTCCCTTCTCCATCCCTT | 60.3 | 0.00 |
| mm4_intron_Cnnm2_chr19_46773154_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Cnnm2_chr19_46773154_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Brd9_chr13_73947795_F | TCGTCGGCAGCGTCGCAAGTTGCTCCCAGGTTTT | 59.2 | 0.00 |
| mm4_intron_Brd9_chr13_73947795_R | GTCTCGTGGGCTCGGGAGGACAGTGTCACATCCCC | 59.7 | 0.00 |
| mm4_intergenic_Gm26468|Rpsa-ps10_chr3_149411739_F | TCGTCGGCAGCGTCAGTCTTCAGTTGCAGTCTGTG | 58.1 | 0.00 |
| mm4_intergenic_Gm26468|Rpsa-ps10_chr3_149411739_R | GTCTCGTGGGCTCGGTCAGTTGACATTAGGCTTGCA | 57.8 | 0.00 |
| mm4_intergenic_A930011G23Rik|Gm16227_chr5_99478177_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_A930011G23Rik|Gm16227_chr5_99478177_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_9430015G10Rik|Rnf223_chr4_156130459_F | TCGTCGGCAGCGTCCCATTCTGAGTCTGCCAGGG | 60.1 | 0.00 |
| mm4_intergenic_9430015G10Rik|Rnf223_chr4_156130459_R | GTCTCGTGGGCTCGGGGGTGTGAATTCTCAGGCCA | 59.9 | 0.00 |
| mm4_exon_Gm17281/Pacsin3_chr2_91256828_F | TCGTCGGCAGCGTCATGCTGTCGGTGGGGTTG | 59.9 | 0.00 |
| mm4_exon_Gm17281/Pacsin3_chr2_91256828_R | GTCTCGTGGGCTCGGCGCTCAGTTCGGAGTCCTAC | 59.9 | 0.00 |
These only list off-targets that have primers in the table above. Primers underlined, off-targets in bold.
| ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52314088 |
GTCGATGGACTAGCAGGCAGTGCTGCAGGGCTGGGCGCCGAGCCACGGTCTGCTGGAGCGGCCATGCTTGGCCCGGGACC CCCAGTCCCCTCCGCGGACAGCCTCTCTGGCCAAGGGCAACCTAGTAGCT |
| mm4_intron_Megf6_chr4_154231802 |
TGGTCCCAGTGATAGGTTGCTGGTCCCAAGCCACAGTCTGTTGGATCCAATCATTGTCCAGATCTCCCACCTTGGCTGCC TGGTATCCAGGGCCCTTCTTCCTCATTGCCT |
| mm4_intergenic_Gm11397|Serpinb9g_chr13_33430464 |
TCTGGACTGCAATGGTTCCCTTTTTTCTCAAGCAGACCATGGCTAGGGCTGAGAAGAAGTTTATGACTTAGAAAAGTACA TTTTTCAACTCTCTTCACCATTATTTTCAAAAGGCTTAAAGCAAAGATGC |
| mm3_intron_Ncf4_chr15_78253683 |
AGCACCCTGTCAACAGTAGCATCCAAGCCATGGTCTGCTGGAAAGCCTTCCTGGCTGTCCAGAGGTCCTTTACAGCTGGA GTGCTCTGGCTTTGGAGAC |
| mm4_intergenic_Serpinb9c|Serpinb9d_chr13_33186180 | TCTGGACTGCAATGGTTCCCTTTTCCCTCATGCAGACCATGGCTAGGGCTGAGAAGAAGTTTATGCCTTAGA |
| mm2_intergenic_Rab27b|Dynap_chr18_70141623 |
AAACCGGCTCAGTCCTTCTGACAGGGTGAGAAACGCTCCAGCAGACAGTGTCTGGGCGGTCCTAGTGCCTGCTCAGGTTA GAAAGCTAGAAGATTCCACAATCTGCTGTGTCTGCCA |
| mm3_intron_Myo18a_chr11_77832606 |
CTAGACACTGCAGGCTGGTGCCTGTTCAAGGTTATCACGTTCCAGCCGACCATGGCTAGGACAGACACAACACTGTGGCA GCCTGAAGTCAGCTCAGAGCACAGGCTGCAGCACAGATTGGAGAGG |
| mm4_intergenic_Gm15405|Gm27039_chr17_92080915 |
CCCAACAAGCCCATTACCCTTCAAGTCTGCACCTTTATTTCTGCATTCCATCTTCTAATTAAGCTAGGATACCAATTACC CAAGCCACTGCCTGCTGGAACCCTAATAAGCCCTGCAGGCTTGAGATCCAA |
| mm4_intergenic_Gm26815|Gm26747_chr8_121377753 |
AGCTGGGTGGATTTACTGCCCCACCCCACCCCCAGCTCTATCAGACCCTGGCTGGGGGGGCTCCCATTTCTCCCTAATCA CTTGCCCTGCA |
| mm4_intron_Acvr1_chr2_58550495 |
AGTCACAGGCTCAGCAACTGGGATGGGGCACAGGGATGCAGACAAGGCTGGGAACCACTCCATCAGAATGTGGCTCGGGA TGGTATTTAAGGGAAAAGGGAAATTCCTATGTATGGTAAACAAACAGAGCACCAGCCT |
| mm3_intron_Ptges2_chr2_32396827 |
CAGACTCAGAACTGGAGGCGTGCTCCAGCCCACCGTGGCTCGGCAGCCCTGTAGACCTTTCATGAGTTGCAGTCTTTAGC CCTTCTCCGCCCTGCCTCCCATGCTCCTTTCCTG |
| mm4_exon_Cdr2_chr7_120958425 |
CTGCTGCTTCGTTTGAGTGGTTTTCTAGGTGCTTCTGGAGCAGCCAGGAACATTTCCTCCAGCAGACTCTGGCTAGGCTC CTTGAAAGGAACAAACAGAGAGTCGGGCACCAGCTTCTCCACACCAT |
| mm4_exon_Slc10a4_chr5_73007033 |
GACGCTTGACCAGACAGACAGGTAAAAGGGACCACAGGACCGAGGGAGGAGGTGTGGCCAGGGACAGCCCCTGCAGACCG TGCCTCGGCGACAGAGCCATGGACAGCCTGGACAACA |
| mm4_intergenic_1810011O10Rik/Gm26714|Ido2_chr8_24509203 |
AACAGCAGGGATGAGAACCGGTCATACCATTTCCCCTTGCCACACTTGAGGCCGCACCTGGCCACTGCCAAGGTTCCAGC AGCCCGTGACTTGGAAGTTTTCAGCACACACTCACCTCT |
| mm4_intron_Rnaseh1_chr12_28654193 |
CAACTGGGCCTGGCTAATCAATTAGTACAAAATTATTAAGCTTTTGGGAATTCTTTCCCTAGCCACAGGCTGCTGGGGCC AGAAGGAGAGTCCAACCAGAATGTCTTTTCTAACTGAGCATTTGTCCGGCCTCAGAACC |
| mm4_intergenic_Fscb|Gm24066_chr12_64588534 |
ACCCCTTCTTTTCTCGTTGCTAATCAAAAATGATCATACTTGCCAACTGGAGGTTATGAGACCTCTCCAGCAGACCATAG CGTGGCAGAAAAAAGGCTGAATATATTGTAGCTACTTTCGAAGCAGAAATGTACCTATGC |
| mm4_intergenic_Arid4b|Gm26129_chr13_14065020 |
GAGTGGCTCGGTGTGGAGCTTTCCCTGACAGGGTCAGCTGGAGCGAACATCCACAGGCTGTGTGTTTCGAAGTTATGGAG TCAGTTAGGAGATAACCTCACCCAAGCTGTTCAG |
| mm4_intron_Tepp_chr8_95314178 | GCTAGGAGCACCCTCTCTCTGTGCCCAACCCACGGTCTGCTTCAGCATGGTTGGATTCACACGCCA |
| mm4_intergenic_Gm26137|Gm23733_chr3_120191336 |
TGAATGTCTGATCTTCTGATAGGGATACTTACTCCTTTTAATGAAGACCAAGCCAAGGTCTTCTGGATAGGCTATATGCT TTAAGAACTAAGCTCCATTCTTCTTGCTATGCT |
| mm4_intergenic_Dbx2|Gm8849_chr15_95703024 |
AGGTGCCCCTTGCTTTGAAATGAGTAGCACCCAGCCACTGTATGCTGGTGCTCATGAGGCAGAGCAATGTTGTAAACACA GAGCTTTTCATTTTTGGTGTTCTTGACCATCACTGTGACCCTGCTCTGGAAC |
| mm4_intergenic_Parp11|Efcab4b_chr6_127565667 | TGTGATGGTGTGTCCTCGTGACAACCAAGCCAGGGTGTGCTGGTGTGTCCTCGTGACAACTAAGCCAGGGT |
| mm4_intron_Cdh22_chr2_165183101 |
TTCCTGGCGCTTATGAGCTCCCTGCATGAGCCTCGCCTGCCTCAGGAACTGTTCCCCTGGCTCCAGCAGACAGTTGCAGG GCTGCATGGCATGGCCCTGCAGCAATGTGGGGGGAGAAGGGACAGGGAAGTGTGG |
| mm4_exon_Pdxk_chr10_78440867 |
GTCCCTTTTGCTCTGGACCATCCTCAGCTCCAGCTGAGCGGGGCTGGGCTTCTGTCCTTCTCCTGCTTCAGCTGCAGAGC ACAGGGAAGATCAGAGCAGGC |
| mm4_intron_E230016M11Rik_chr6_67072343 |
CCAACCAGTGAAGGTCGACAGTACTCCTCAGTGACTTGTCCTGGTCACCAAGCCAGTGTGTGCTGGAGCTAGCACTGAGA TGAGCTCCTTGCCCCCAGCTCACTCAGCTTGGCATGCTCTT |
| mm4_intron_Atoh8_chr6_72229939 |
TCCACTCCTCCTCTACTGCCCAAGCTAGACCCTCCCTTGGCTCCAGCATACACTGGCTGGGCAGCCTGAGGGCCTGGCTG TCCTCTGGACATCTTCCCGACTTTAAGGAGGACTGTGGCCTTACAGGACCCCTTTC |
| mm4_intron_Adck1_chr12_88445858 |
TTCCCAGGTGCAGAAACTGTAGCAGGGTTAGGACCAAGCAAGGGTCTGATGGAGGGAGAGGCAGAGGGAAGCAGCTCTTA GCAC |
| mm4_intron_Unc5d_chr8_28720637 |
ACTCTAAGTCTGAGGGGCCAATGTACTTGAACATCACAGATGTCACTCTGTGAGGGAAAGAATGCTGCAGCAGAGAGTGG CTTGGATTGCAAGTGGGAGCATCTCAG |
| mm4_intergenic_Gm9992|Gm25872_chr17_7582772 |
TGCTCATCCGGATCCAAAGTCCAAACTGAATGGACCACTCTGAGTCCATTCTCCTTCCTTCCCACGGTCTGCTGAAGAGG TCTCATGGTGACAGGAAGCCTGGACAGTGGACATTAGTTTAGCTGAGTTGGGTGGGGAT |
| mm4_exon_Baiap3_chr17_25245948 | CCTCTCACCTGCTCACTCACTGCCTCACCAGCAGCCCCTGGCTGGGTGTCCACCTTCTTCCTCAGCAGTTCCGTGT |
| mm4_intergenic_RP23-93M1.2|RP24-308I2.1_chr9_32886364 |
TGTCTGAACTGCCACAGTGCCTCTGAAGGTCCCTCCTGCAGCCCCTGGCTGGGCTCTCAGCCTAGAGAGGTTATGAGGCA CATGGAAAGCAAATTATTTGCACAGATCCAGCTAATGAATAGCTTTGGACAGGGGACTTC |
| mm4_intron_Nav2_chr7_48998426 |
GTGGAGCAGAAAGGGACCTTCTCGCTCAAGCAGAGCCTGCCTGGGGGTAATGTGTGAGCTTTGAGGAAGCTGCCCAGGCT CTGAAGCTGTCTGGCCAG |
| mm4_intron_Chn2_chr6_54040216 |
TGGAGAGTCTACCTGTGGCCGTCCCTCCCGCTCTAGGAGAGCGTGGCGCGGGACACGAGCGTCACTGGGACACTTGTTAA GGTGGTCAACACCTTTGCGTC |
| mm4_intergenic_Zfp516|Gm24720_chr18_83237038 |
TCTGTCTGCACTCACAGCTGGCCACCCCACGGTCTGCTGGAAGGAACTCAGGAAGTGTCAGTGTGACCCAATGCTTTGTT TCACCTATTCTGAGCATCCTCTGTGAACCTGACTTCAGCATCGAGGG |
| mm4_intergenic_Cdh4|Gm14300_chr2_179525563 |
CAAAGTGAGGGCAGGTCTGTCCTGGCCAGGGTCTGCAGGAGCCGAAGGAGTTTTTCCTAGAGCAAGGTTTGAGCAGTCTA CCTCCTGGGTTGGTGCTCCTGGAGTGAG |
| mm4_intergenic_Gm7551|Gm20684_chr7_65803481 |
TGGCTTCATGCATGGTGTCTGCCCAGGAGGGTGGATGAGGCCAGGATAGCCTGGCCAGGGTCTGCAGGAGTGAGGGCTGA TGATCTTGCCAG |
| mm4_intergenic_AI606473|Tyw3_chr3_154540836 |
GGATATGACATCGCAGCCCATCTGGTGACAGGATCCAGGCCCAGGTCTGCTGGAGCTTTGAGAGCTGGGGAAGAGGGAAG GGAGCCAGGTGCAGCTGAGGAAGGGATGGAGAAGGGAGGG |
| mm4_intron_Brd9_chr13_73947795 |
GCAAGTTGCTCCCAGGTTTTACAACATTGGGTTTCAAAGATGAAAGAAGAAATAAAGGTAAGCACAGTGACTGATAGGAG CTCTCCAGCTGAGGGTGGCTGGGCTCAGTGGGGATGTGACACTGTCCTC |
| mm4_intergenic_Gm26468|Rpsa-ps10_chr3_149411739 |
AGTCTTCAGTTGCAGTCTGTGCTGAACACATTTGCAGATCATTAGCCCCAGCCACCGTCTGCTTCACCGGGAATCAGTTT TTATTGAATTCAAACTTCCAAAAGGAAAATATGCAAGCCTAATGTCAACTGA |
| mm4_intergenic_9430015G10Rik|Rnf223_chr4_156130459 |
CCATTCTGAGTCTGCCAGGGAAGAGAAGAGCCAATCGCTGATGTTCGGGGCCACACCCCCAGCCACCGTGTCCTGGAGGG AATAAATAAACCTGCTTCAAAACTCATGGCCTGAGAATTCACACCC |
| mm4_exon_Gm17281/Pacsin3_chr2_91256828 |
ATGCTGTCGGTGGGGTTGGGGCGGCGGCTCCGCGGAGANNNNNNNNNNNNNNNNNNNNNNTTCCCGGGGGAGGGCCCGAA GCGCTCCAGGAGGCGGTGGCCCGGACCCGGGAGTGGTAGGACTCCGAACTGAGCG |