CRISPOR

DNA break repair outcome predictions

Guide: TAGGTTGCCCTTGGCCAGAG AGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

mh- Score	Sequence	Effect
	`CGATTCGGTGTCTGAGCTACTAGGTTGCCCTTGGCCAGAGAGGCTGTCCGCGGAGGGGACTGGGGGTCCCGGGCCAAGCA`	(Wild-type sequence)
382	`TCTGAGCTACTAGGTTGCCCTTGGC---------TGTCCGCGGAGGGGACTGGGGGTCCC`	`9 bp deleted → no frameshift`
271	`TCTGAGCTACTAGGTTGCCCTTGGCCAGAG--GCTGTCCGCGGAGGGGACTGGGGGTCCC`	`2 bp deleted → frameshift`
245	`TCTGAGCTACTAGGTTGCCCTTGGCCAG----GCTGTCCGCGGAGGGGACTGGGGGTCCC`	`4 bp deleted → frameshift`
235	`TCTGAGCTACTAGGTTGCCCTTGGCCAGAG---------------GGGACTGGGGGTCCC`	`15 bp deleted → no frameshift`
208	`TCTGAGCTACTAGGTTGCCCTTGGCC-------------GCGGAGGGGACTGGGGGTCCC`	`13 bp deleted → frameshift`
193	`TCTGAGCTACTAGG-------------------CTGTCCGCGGAGGGGACTGGGGGTCCC`	`19 bp deleted → frameshift`
179	`TCTGAGCTACTAGGTTGCCCTTGGC----------------GGAGGGGACTGGGGGTCCC`	`16 bp deleted → frameshift`
179	`TCTGAGCTACTAGGTTGC----------------TGTCCGCGGAGGGGACTGGGGGTCCC`	`16 bp deleted → frameshift`
156	`TCTGAGCTACTAGGTTGCCCTTG-------------TCCGCGGAGGGGACTGGGGGTCCC`	`13 bp deleted → frameshift`
154	`TCTGAGCTACTAGGTTGCCCTTGG-------------------AGGGGACTGGGGGTCCC`	`19 bp deleted → frameshift`
154	`TCTGAGCTACTAGGTTGCCC-------------------GCGGAGGGGACTGGGGGTCCC`	`19 bp deleted → frameshift`
149	`TCTGAGCTACTAGGTTGCCCT--------------GTCCGCGGAGGGGACTGGGGGTCCC`	`14 bp deleted → frameshift`
147	`TCTGAGCTACTAGGTTGCC--------------------GCGGAGGGGACTGGGGGTCCC`	`20 bp deleted → frameshift`
133	`TCTGAGCTACTAGGTTGCCCTTGG----------------------GGACTGGGGGTCCC`	`22 bp deleted → frameshift`
129	`TCTGAGCT---------------------------GTCCGCGGAGGGGACTGGGGGTCCC`	`27 bp deleted → no frameshift`
128	`TCTGAGCTACTAGGTTGCCCTTGGCCAG-----------------GGGACTGGGGGTCCC`	`17 bp deleted → frameshift`
126	`TCTGAGCTACTAGGTTGCCCTTGG-----------------------GACTGGGGGTCCC`	`23 bp deleted → frameshift`
126	`TCTGAGCTACTAGGTTGC-----------------------GGAGGGGACTGGGGGTCCC`	`23 bp deleted → frameshift`
120	`TCTGAGCTACTAGGTTGCCCTTGG------------------------ACTGGGGGTCCC`	`24 bp deleted → no frameshift`
117	`TCTGAGCTACTAGGTTGCCCTTGG-----------------------------GGGTCCC`	`29 bp deleted → frameshift`
116	`TCTGAGCTACTAGGTTG-------------------TCCGCGGAGGGGACTGGGGGTCCC`	`19 bp deleted → frameshift`
110	`TCTGAGCTACTAGGTTGCCCTTGGCCAGA--------------------CTGGGGGTCCC`	`20 bp deleted → frameshift`
101	`TCTGAGCTACTAGG--------------------------------GGACTGGGGGTCCC`	`32 bp deleted → frameshift`
101	`TCTG--------------------------------TCCGCGGAGGGGACTGGGGGTCCC`	`32 bp deleted → frameshift`
99	`TCTGAGCTACTAGGT----------------------CCGCGGAGGGGACTGGGGGTCCC`	`22 bp deleted → frameshift`
94	`TCTGAGCTACTAGG-----------------------------AGGGGACTGGGGGTCCC`	`29 bp deleted → frameshift`
90	`TCTGAGCTACT------------------------GTCCGCGGAGGGGACTGGGGGTCCC`	`24 bp deleted → no frameshift`
89	`TCTGAGCTACTAGGTTGCCCTTGG------------------------------GGTCCC`	`30 bp deleted → no frameshift`
84	`TCTGAGCTACTAGGTTGCCCTTGG-------------------------------GTCCC`	`31 bp deleted → frameshift`
81	`TCTGAG--------------------------GCTGTCCGCGGAGGGGACTGGGGGTCCC`	`26 bp deleted → frameshift`
81	`TCTGAGCTACTAGGTTGCCC----------------------------------------`	`40 bp deleted → frameshift`
80	`TCTGAGCTACTAGGTTGCCCTTGG--------------------------------TCCC`	`32 bp deleted → frameshift`
76	`TCTGAGCTACTAGGTTGCCCTTGGCC---------------------------------C`	`33 bp deleted → no frameshift`
76	`TCTGAGCTACTAGG---------------------------------GACTGGGGGTCCC`	`33 bp deleted → no frameshift`
73	`TCTGAGCTACTAGGTTGCCCTTGGCC----------------------------------`	`34 bp deleted → frameshift`
73	`TCTGAGCTACTAGG----------------------------------ACTGGGGGTCCC`	`34 bp deleted → frameshift`
73	`TCTGAGC----------------------------------GGAGGGGACTGGGGGTCCC`	`34 bp deleted → frameshift`
71	`TCTGAG---------------------------------------GGGACTGGGGGTCCC`	`39 bp deleted → no frameshift`
66	`TCTGAGCTACTAGGTTGCCCT------------------------------GGGGGTCCC`	`30 bp deleted → no frameshift`
61	`TCTGAGCTACTAGGT------------------------------------------CCC`	`42 bp deleted → no frameshift`
56	`TCTGAGCTACTAGGTTGCCC---------------------------------------C`	`39 bp deleted → no frameshift`
56	`TCTGAGCTACTAGG---------------------------------------GGGTCCC`	`39 bp deleted → no frameshift`
54	`TCTGAGCTACTAGG----------------------------------------GGTCCC`	`40 bp deleted → frameshift`
54	`TCTGAGCTACT----------------------------------------GGGGGTCCC`	`40 bp deleted → frameshift`
52	`TCTGAGCTACTAGGTTG-----------------------------------GGGGTCCC`	`35 bp deleted → frameshift`
51	`TCTGAGCTACTAGGTTGCC-----------------------------------------`	`41 bp deleted → frameshift`
51	`TCTGAGCTACTAGG-----------------------------------------GTCCC`	`41 bp deleted → frameshift`
49	`TC------------------------------------CGCGGAGGGGACTGGGGGTCCC`	`36 bp deleted → no frameshift`
45	`TCTG------------------------------------------------GGGGTCCC`	`48 bp deleted → no frameshift`
34	`TCTGAGCT-------------------------------------------GGGGGTCCC`	`43 bp deleted → frameshift`
33	`TCTGA--------------------------------------------CTGGGGGTCCC`	`44 bp deleted → frameshift`
18	`TC--------------------------------------------------------CC`	`56 bp deleted → frameshift`

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