CRISPOR

DNA break repair outcome predictions

Guide: TGGCGGGGGAGGCCAGGAGG AGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

mh- Score	Sequence	Effect
	`CCTCCGACTTCCCGGGAGCGTGGCGGGGGAGGCCAGGAGGAGGAGCCGGTCGATGGACTAGCAGGCAGTGCTGCAGGGCT`	(Wild-type sequence)
592	`CCCGGGAGCGTGGCGGGGGAGGCCAGGAG------CCGGTCGATGGACTAGCAGGCAGTG`	`6 bp deleted → no frameshift`
430	`CCCGGGAGCGTGGCGGGGGAGGCCAGGAGG---AGCCGGTCGATGGACTAGCAGGCAGTG`	`3 bp deleted → no frameshift`
347	`CCCGGGAGCGTGGCGGGGGAG--------------CCGGTCGATGGACTAGCAGGCAGTG`	`14 bp deleted → frameshift`
313	`CCCGGGAGCGTGGCGGGGGAGGCC-------------GGTCGATGGACTAGCAGGCAGTG`	`13 bp deleted → frameshift`
288	`CCCGGGAGCGTGGCGGGGGAGG-----------AGCCGGTCGATGGACTAGCAGGCAGTG`	`11 bp deleted → frameshift`
255	`CCCGGGAGCGTGGCGGGGGAGGCCAGGAGG---------TCGATGGACTAGCAGGCAGTG`	`9 bp deleted → no frameshift`
233	`CCCGGGAGC---------------------------CGGTCGATGGACTAGCAGGCAGTG`	`27 bp deleted → no frameshift`
219	`CCCGGGAGCGTGGCGGGGGAGGCCAGG------------TCGATGGACTAGCAGGCAGTG`	`12 bp deleted → no frameshift`
193	`CCCGGGAGCGTGGCGGGGGAGGCCAGGA-------------------CTAGCAGGCAGTG`	`19 bp deleted → frameshift`
191	`CCCGGGAGCGTGGCGGGGGAGGCCAG---------CCGGTCGATGGACTAGCAGGCAGTG`	`9 bp deleted → no frameshift`
190	`CCCGGGAGCGTGGCGG-----------------------TCGATGGACTAGCAGGCAGTG`	`23 bp deleted → frameshift`
188	`CCCGGGAGCGTGGCGGGG---------------AGCCGGTCGATGGACTAGCAGGCAGTG`	`15 bp deleted → no frameshift`
179	`CCCGGGAGCGTGGCGGGGGAGGCCAGGAGG----------------ACTAGCAGGCAGTG`	`16 bp deleted → frameshift`
179	`CCCGGGAGCGTGGCGGG----------------AGCCGGTCGATGGACTAGCAGGCAGTG`	`16 bp deleted → frameshift`
172	`CCCGGGAGCGTGGCGGGGGAGGCCAGG----------------------------CAGTG`	`28 bp deleted → frameshift`
170	`CCCGGGAGCGTGGCGGGGGAGG-----------------TCGATGGACTAGCAGGCAGTG`	`17 bp deleted → frameshift`
170	`CCCGGGAGCGTGGCGG-----------------AGCCGGTCGATGGACTAGCAGGCAGTG`	`17 bp deleted → frameshift`
147	`CCCGGGAGCGTGGCGGGGG--------------------TCGATGGACTAGCAGGCAGTG`	`20 bp deleted → frameshift`
147	`CCCGGGAGCGTGG--------------------AGCCGGTCGATGGACTAGCAGGCAGTG`	`20 bp deleted → frameshift`
146	`CCCGG----------------------------------TCGATGGACTAGCAGGCAGTG`	`34 bp deleted → frameshift`
143	`CCCGGGAGCGTGGCGGGGGAGGCCAGGAGG-------------------------CAGTG`	`25 bp deleted → frameshift`
141	`CCCGGGAGCGTGGCGGGGGAGGCCAGGA---------------TGGACTAGCAGGCAGTG`	`15 bp deleted → no frameshift`
140	`CCCGGGAGCGTGGCGGGG---------------------TCGATGGACTAGCAGGCAGTG`	`21 bp deleted → no frameshift`
134	`CCCGGGAGCGTGGCGGGGGAGGC---------------------------------AGTG`	`33 bp deleted → no frameshift`
133	`CCCGGGAGCGTGGCGGG----------------------TCGATGGACTAGCAGGCAGTG`	`22 bp deleted → frameshift`
133	`CCCGGGAGCGTGGC----------------------CGGTCGATGGACTAGCAGGCAGTG`	`22 bp deleted → frameshift`
129	`CCCGGGAGCGTGGCGGGGGA---------------------------CTAGCAGGCAGTG`	`27 bp deleted → no frameshift`
120	`CCCGGGAGCGTGGCGGGGGAGG------------------------ACTAGCAGGCAGTG`	`24 bp deleted → no frameshift`
109	`CCCGGGAGCGTGG--------------------------TCGATGGACTAGCAGGCAGTG`	`26 bp deleted → frameshift`
103	`CCCGGGAGCGTGGCG---------------------------ATGGACTAGCAGGCAGTG`	`27 bp deleted → no frameshift`
101	`CCCGGGAGCGTGGCGGGGGAGGCCAG--------------------------------TG`	`32 bp deleted → frameshift`
99	`CCCGGGAGCGTGGCGGGGGAGGCCAGGAG----------------------CAGGCAGTG`	`22 bp deleted → frameshift`
98	`CCCGGGAGCGTGGCGGGG----------------------------ACTAGCAGGCAGTG`	`28 bp deleted → frameshift`
98	`CCCGGGAGCG----------------------------GTCGATGGACTAGCAGGCAGTG`	`28 bp deleted → frameshift`
98	`CCCGG----------------------------AGCCGGTCGATGGACTAGCAGGCAGTG`	`28 bp deleted → frameshift`
96	`CCCGGGAGCGTGG---------------------------------ACTAGCAGGCAGTG`	`33 bp deleted → no frameshift`
95	`CCCGGGAGCGTGGCGGGGGA-----------------------TGGACTAGCAGGCAGTG`	`23 bp deleted → frameshift`
94	`CCCGGGAGCGTGGCGGGGGAGGC-----------------------------AGGCAGTG`	`29 bp deleted → frameshift`
94	`CCCGGGAGCGTGGCGGG-----------------------------ACTAGCAGGCAGTG`	`29 bp deleted → frameshift`
90	`CCCGGGAG------------------------GAGCCGGTCGATGGACTAGCAGGCAGTG`	`24 bp deleted → no frameshift`
89	`CCCGGGAGCGTGGCGG------------------------------ACTAGCAGGCAGTG`	`30 bp deleted → no frameshift`
86	`CCCGGGAGCGTGGCGGGGGAGGCCAG-------------------------CAGGCAGTG`	`25 bp deleted → frameshift`
80	`CCCGGGAGCG--------------------------------ATGGACTAGCAGGCAGTG`	`32 bp deleted → frameshift`
76	`CCCGGG---------------------------------TCGATGGACTAGCAGGCAGTG`	`33 bp deleted → no frameshift`
73	`CCCGGGAGCGTGGC------------------------------------------AGTG`	`42 bp deleted → no frameshift`
70	`CCCGGGAGCGTGGCGGGGGAGGCCAGGAG-----------------------------TG`	`29 bp deleted → frameshift`
70	`CCCGGGAGCGT-----------------------------CGATGGACTAGCAGGCAGTG`	`29 bp deleted → frameshift`
69	`CC-----------------------------------GGTCGATGGACTAGCAGGCAGTG`	`35 bp deleted → frameshift`
67	`CCCGGGA----------------------------------------CTAGCAGGCAGTG`	`40 bp deleted → frameshift`
66	`CCCGGGAGCGTGGCGGGGGAG------------------------------CAGGCAGTG`	`30 bp deleted → no frameshift`
66	`CCCGGGAGCGTGGCGGGGG------------------------------------CAGTG`	`36 bp deleted → no frameshift`
62	`CCCGGGAGCGTGGCGGGG-------------------------------------CAGTG`	`37 bp deleted → frameshift`
60	`CCCGGGAGCGTGGCGGG--------------------------------------CAGTG`	`38 bp deleted → frameshift`
60	`CCCGGGAGCGTGGC--------------------------------------AGGCAGTG`	`38 bp deleted → frameshift`
60	`CCCG--------------------------------------ATGGACTAGCAGGCAGTG`	`38 bp deleted → frameshift`
58	`CCCGGGAGC-------------------------------------------AGGCAGTG`	`43 bp deleted → frameshift`
56	`CCCGGGAGCGTGGCGG---------------------------------------CAGTG`	`39 bp deleted → no frameshift`
51	`CCCGG-----------------------------------------ACTAGCAGGCAGTG`	`41 bp deleted → frameshift`
49	`CCCGGGA------------------------------------TGGACTAGCAGGCAGTG`	`36 bp deleted → no frameshift`
47	`CCCGGGAGCGTGGCGGGGGAG-------------------------------------TG`	`37 bp deleted → frameshift`
45	`CCCGGGAGCGTG------------------------------------------------`	`48 bp deleted → no frameshift`
38	`CCCGGGAGC-----------------------------------------------AGTG`	`47 bp deleted → frameshift`
34	`CCCGGG-------------------------------------------------CAGTG`	`49 bp deleted → frameshift`
32	`CCCGG--------------------------------------------------CAGTG`	`50 bp deleted → frameshift`
30	`CCCGGGAG----------------------------------------------GCAGTG`	`46 bp deleted → frameshift`
24	`CCCGGGAG--------------------------------------------------TG`	`50 bp deleted → frameshift`

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