CRISPOR

DNA break repair outcome predictions

Guide: CCAGAGAAGATGGTGCCCCA CGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

mh- Score	Sequence	Effect
	`GCAGCCCGCTGCCTGAACCGCCAGAGAAGATGGTGCCCCACGGTTGCCTGAGCCCGCGAGCCGGCCCTCCGACTTCCCGG`	(Wild-type sequence)
403	`GCCTGAACCGCCAGAGAAGATGGTGCC-----------TGAGCCCGCGAGCCGGCCCTCC`	`11 bp deleted → frameshift`
341	`GCCTGAACCGCCAGAGAAGATGGTGCCC-----------------GCGAGCCGGCCCTCC`	`17 bp deleted → frameshift`
303	`GCCTGAACCGCCAGAGAAGATGGT----------TGCCTGAGCCCGCGAGCCGGCCCTCC`	`10 bp deleted → frameshift`
269	`GCCTGAACCGCCAGAGAAGATGGTGCCCC----------------GCGAGCCGGCCCTCC`	`16 bp deleted → frameshift`
255	`GCCTGAACCGCCAGAGAAGATGGTGCCCC---------TGAGCCCGCGAGCCGGCCCTCC`	`9 bp deleted → no frameshift`
242	`GCCTGAACCGCCAGAGAAGATGGTGCCC----------TGAGCCCGCGAGCCGGCCCTCC`	`10 bp deleted → frameshift`
188	`GCCTGAACCGCCAGAGAAGATGGTGCCCC---------------CGCGAGCCGGCCCTCC`	`15 bp deleted → no frameshift`
188	`GCCTGAACCGCCAGAGAAGATGGTGCCC-----------------------------TCC`	`29 bp deleted → frameshift`
174	`GCCTGA-----------------------------------GCCCGCGAGCCGGCCCTCC`	`35 bp deleted → frameshift`
172	`GCCTGAACCGCCAGAGAAGATGGTGCC-------------------------GGCCCTCC`	`25 bp deleted → frameshift`
163	`GCCTGAACCGCC--------------------------TGAGCCCGCGAGCCGGCCCTCC`	`26 bp deleted → frameshift`
162	`GCCTGAACCGCCAGAGAAGATGGTGCC------------------GCGAGCCGGCCCTCC`	`18 bp deleted → no frameshift`
149	`GCCTGAACCGCCAGAGAAGATG--------------CCTGAGCCCGCGAGCCGGCCCTCC`	`14 bp deleted → frameshift`
148	`GCCTGAACCGCCAGAGAAGATGGTGCCCC----------------------------TCC`	`28 bp deleted → frameshift`
141	`GCCTGAACCGCCAGAGAAGATGGTG---------------AGCCCGCGAGCCGGCCCTCC`	`15 bp deleted → no frameshift`
140	`GCCTGAACCGCCAGAGAAGATGGTGC---------------------GAGCCGGCCCTCC`	`21 bp deleted → no frameshift`
136	`GCCTGAACCGCCAGAG--------------------------CCCGCGAGCCGGCCCTCC`	`26 bp deleted → frameshift`
133	`GCCTGAACCG----------------------GTTGCCTGAGCCCGCGAGCCGGCCCTCC`	`22 bp deleted → frameshift`
132	`GCCTGAACCGC------------------------------------GAGCCGGCCCTCC`	`36 bp deleted → no frameshift`
126	`GCCTGAACCGCCAGAGAAGATGGTGCCCC-----------------------GGCCCTCC`	`23 bp deleted → frameshift`
122	`GCCTGAACCGCCAGAGAAGATG------------------AGCCCGCGAGCCGGCCCTCC`	`18 bp deleted → no frameshift`
121	`GCCTGAACCGCC--------------------------------CGCGAGCCGGCCCTCC`	`32 bp deleted → frameshift`
120	`GCCTGAACCGCCAGAGAAGATGGTGCCC------------------------GGCCCTCC`	`24 bp deleted → no frameshift`
105	`GCCTGAACCGCCAGAGAAGA---------------------GCCCGCGAGCCGGCCCTCC`	`21 bp deleted → no frameshift`
103	`GCCTGAACCGCCAGAGAAGATGGTGCCCC---------------------------CTCC`	`27 bp deleted → no frameshift`
95	`GCCTGAACCGCCAGAGAAG-----------------------CCCGCGAGCCGGCCCTCC`	`23 bp deleted → frameshift`
94	`GCCTGAACC-----------------------------TGAGCCCGCGAGCCGGCCCTCC`	`29 bp deleted → frameshift`
91	`GCCTGAACCGCCAGAG----------------------------------CCGGCCCTCC`	`34 bp deleted → frameshift`
90	`GCCTGAACCGCCAGAGA------------------------GCCCGCGAGCCGGCCCTCC`	`24 bp deleted → no frameshift`
89	`GCCTGAACCGCCAGAGAAGATGGTGCC------------------------------TCC`	`30 bp deleted → no frameshift`
84	`GCCTGAACCGCCAGAGAAGATGGTGCCCC-------------------------------`	`31 bp deleted → frameshift`
84	`GCCTGAACCGCCAGAGAAGATGG-------------------------------CCCTCC`	`31 bp deleted → frameshift`
81	`GCCTGAACCGCC----------------------------------------GGCCCTCC`	`40 bp deleted → frameshift`
80	`GCCTGAACCGCCAGAGAAGATGGTGCCC--------------------------------`	`32 bp deleted → frameshift`
77	`GCC-----------------------------------------CGCGAGCCGGCCCTCC`	`41 bp deleted → frameshift`
76	`GCCTGAACCGCCAGAGAAGATGGTGCC---------------------------------`	`33 bp deleted → no frameshift`
76	`GCCTGAACCGCC---------------------------------GCGAGCCGGCCCTCC`	`33 bp deleted → no frameshift`
74	`GCCTGAACCGCCAG----------------------------CCCGCGAGCCGGCCCTCC`	`28 bp deleted → frameshift`
70	`GCCTGAACCGCCAGAGAAGA-----------------------------GCCGGCCCTCC`	`29 bp deleted → frameshift`
69	`GCCTGAACCG-------------------------------------------GCCCTCC`	`43 bp deleted → frameshift`
69	`GCCTGAACC-----------------------------------CGCGAGCCGGCCCTCC`	`35 bp deleted → frameshift`
66	`GCCTGAACCGCC--------------------------------------------CTCC`	`44 bp deleted → frameshift`
63	`GCCTGAACCGCCAGAGAAG-------------------------------CCGGCCCTCC`	`31 bp deleted → frameshift`
63	`GCCTG-------------------------------CCTGAGCCCGCGAGCCGGCCCTCC`	`31 bp deleted → frameshift`
60	`GCCTGAACCGCCAGAGA--------------------------------GCCGGCCCTCC`	`32 bp deleted → frameshift`
60	`GCCTGAACCG--------------------------------------AGCCGGCCCTCC`	`38 bp deleted → frameshift`
51	`GCC-------------------------------------------------GGCCCTCC`	`49 bp deleted → frameshift`
49	`GCCTGAACCGCCAG------------------------------------CCGGCCCTCC`	`36 bp deleted → no frameshift`
48	`GCC------------------------------------------GCGAGCCGGCCCTCC`	`42 bp deleted → no frameshift`
42	`GCC-----------------------------------------------------CTCC`	`53 bp deleted → frameshift`
42	`GCCTGAACCGCC---------------------------------------------TCC`	`45 bp deleted → no frameshift`
42	`GC---------------------------------------------GAGCCGGCCCTCC`	`45 bp deleted → no frameshift`
38	`GCCTGAACC-----------------------------------------------CTCC`	`47 bp deleted → frameshift`
36	`GCCTGAACCGCC------------------------------------------------`	`48 bp deleted → no frameshift`
36	`GCCTGAACC------------------------------------------------TCC`	`48 bp deleted → no frameshift`
34	`GCCTGA-------------------------------------------GCCGGCCCTCC`	`43 bp deleted → frameshift`
33	`GCCT------------------------------------------------------CC`	`54 bp deleted → no frameshift`
31	`GCCTGAACC---------------------------------------------------`	`51 bp deleted → no frameshift`
23	`GCC---------------------------------------------------------`	`57 bp deleted → no frameshift`

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