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The following table lists possible deletions.
Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.
| mh- Score | Sequence | Effect |
|---|---|---|
| GCCGAAAGGACATGGTTATGTTTCTGGATGGGGGTCAGCTTGGCACTCTGGTTGGTAAGAGGGTCTCTAATTTGTCCGAA | (Wild-type sequence) | |
| 311 | CATGGTTATGTTTCTGGATGGGGGTCAGC-----ACTCTGGTTGGTAAGAGGGTCTCTAA | 5 bp deleted → frameshift |
| 274 | CATGGTTATGTTTCTGGATGG------------CACTCTGGTTGGTAAGAGGGTCTCTAA | 12 bp deleted → no frameshift |
| 255 | CATGGTTATGTTTCTGGATGGGGG---------CACTCTGGTTGGTAAGAGGGTCTCTAA | 9 bp deleted → no frameshift |
| 242 | CATGGTTATGTTTCTGGATGGGG----------CACTCTGGTTGGTAAGAGGGTCTCTAA | 10 bp deleted → frameshift |
| 240 | CATGGTTATGTTTCTGG------------------------TTGGTAAGAGGGTCTCTAA | 24 bp deleted → no frameshift |
| 230 | CATGGTTATGTTTCTGGATGGG-----------CACTCTGGTTGGTAAGAGGGTCTCTAA | 11 bp deleted → frameshift |
| 224 | CATGGTTATGTTTCTGG----------------CACTCTGGTTGGTAAGAGGGTCTCTAA | 16 bp deleted → frameshift |
| 213 | CATGGTTATGTTTCTGGATGGGGGT-----------------TGGTAAGAGGGTCTCTAA | 17 bp deleted → frameshift |
| 211 | CATGGTTATGTTTCTGGATGGGGGTCAGCT-------CTGGTTGGTAAGAGGGTCTCTAA | 7 bp deleted → frameshift |
| 211 | CATGGTTATGTTTCTGGATGGGGGTC-----------------------------TCTAA | 29 bp deleted → frameshift |
| 201 | CATGGTTATGTTTCTGGATGGGGGTCA--------CTCTGGTTGGTAAGAGGGTCTCTAA | 8 bp deleted → frameshift |
| 191 | CATGGTTATGTTTCTGGATGGGGGTCAGCT---------GGTTGGTAAGAGGGTCTCTAA | 9 bp deleted → no frameshift |
| 184 | CATGGTTATGTTTCTGGATGG--------------------TTGGTAAGAGGGTCTCTAA | 20 bp deleted → frameshift |
| 175 | CATGGTTATGTTTCTGGATGGGGGT---------------------AAGAGGGTCTCTAA | 21 bp deleted → no frameshift |
| 164 | CATGGTTATGTTTCTGGATGGGGGTC------------TGGTTGGTAAGAGGGTCTCTAA | 12 bp deleted → no frameshift |
| 162 | CATGGTTATGTTTCTGGATGGGG------------------TTGGTAAGAGGGTCTCTAA | 18 bp deleted → no frameshift |
| 154 | CATGGTTATGTTTCTGGATGGG-------------------TTGGTAAGAGGGTCTCTAA | 19 bp deleted → frameshift |
| 150 | CATGGTTATGTTTCTGGATGG------------------------TAAGAGGGTCTCTAA | 24 bp deleted → no frameshift |
| 133 | CATGGTTATGTTTCTGGATGGGG------------------------------TCTCTAA | 30 bp deleted → no frameshift |
| 133 | CATGGTTATGTTTCTGGATGGGG----------------------TAAGAGGGTCTCTAA | 22 bp deleted → frameshift |
| 127 | CATGGTTATGTTTCTGGATGGG-------------------------------TCTCTAA | 31 bp deleted → frameshift |
| 126 | CATGGTTATGTTTCTGGATGGG-----------------------TAAGAGGGTCTCTAA | 23 bp deleted → frameshift |
| 123 | CATGGTTATGTTTCTGG----------------------------TAAGAGGGTCTCTAA | 28 bp deleted → frameshift |
| 123 | CATGG----------------------------CACTCTGGTTGGTAAGAGGGTCTCTAA | 28 bp deleted → frameshift |
| 115 | CATGGTT------------------------------------GGTAAGAGGGTCTCTAA | 36 bp deleted → no frameshift |
| 105 | CATGGTTATGTTTCTGGATGGGGGTCAG---------------------AGGGTCTCTAA | 21 bp deleted → no frameshift |
| 99 | CATGGTTATGTTTCT----------------------CTGGTTGGTAAGAGGGTCTCTAA | 22 bp deleted → frameshift |
| 99 | CATGGTTATG----------------------GCACTCTGGTTGGTAAGAGGGTCTCTAA | 22 bp deleted → frameshift |
| 98 | CATGGTTATGTTTCTGGATGGGGG----------------------------GTCTCTAA | 28 bp deleted → frameshift |
| 95 | CATGGTTATGTTTCTGGATGGGGGTCAG-----------------------GGTCTCTAA | 23 bp deleted → frameshift |
| 84 | CATGGTTATGTT-------------------------------GGTAAGAGGGTCTCTAA | 31 bp deleted → frameshift |
| 81 | CATGGTTATGTTTCTGGATGGGGGTCAGCT--------------------------CTAA | 26 bp deleted → frameshift |
| 81 | CATGGT----------------------------------------AAGAGGGTCTCTAA | 40 bp deleted → frameshift |
| 80 | CATGGTTATGTTTCTGGATGG--------------------------------TCTCTAA | 32 bp deleted → frameshift |
| 74 | CATGGTTATGTTTCTGGATGGGGGTCAGCT----------------------------AA | 28 bp deleted → frameshift |
| 69 | CATGGTTATGTTTCTGG-----------------------------------GTCTCTAA | 35 bp deleted → frameshift |
| 66 | CATGGTTATG------------------------------GTTGGTAAGAGGGTCTCTAA | 30 bp deleted → no frameshift |
| 66 | CATGGTTATGTTTCTGG------------------------------------TCTCTAA | 36 bp deleted → no frameshift |
| 63 | CATGGTTATGTTTCTGGATGGGGGTC-------------------------------TAA | 31 bp deleted → frameshift |
| 60 | CATGGTTATGTTTCTGGA--------------------------------GGGTCTCTAA | 32 bp deleted → frameshift |
| 57 | CA---------------------------------CTCTGGTTGGTAAGAGGGTCTCTAA | 33 bp deleted → no frameshift |
| 54 | CATGGTTATG----------------------------------GTAAGAGGGTCTCTAA | 34 bp deleted → frameshift |
| 52 | CATGGTTATGT-----------------------------------AAGAGGGTCTCTAA | 35 bp deleted → frameshift |
| 51 | CATGGTTATGTTTCT-----------------------------------------CTAA | 41 bp deleted → frameshift |
| 46 | CATGGTTATGTTTCT-------------------------------------------AA | 43 bp deleted → frameshift |
| 45 | CATGGT------------------------------------------------CTCTAA | 48 bp deleted → no frameshift |
| 44 | CATGGTTATGTTT------------------------------GGTAAGAGGGTCTCTAA | 30 bp deleted → no frameshift |
| 38 | CATGG-----------------------------------------------GTCTCTAA | 47 bp deleted → frameshift |
| 34 | CATGGTTATGT-------------------------------------------CTCTAA | 43 bp deleted → frameshift |
| 28 | CATGGTTA---------------------------------------AGAGGGTCTCTAA | 39 bp deleted → no frameshift |
| 15 | CATGGTTA---------------------------------------------------A | 51 bp deleted → no frameshift |